TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - O'Connor, Kevin E.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1319
AB  - Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
VL  - 95
IS  - 3
SP  - 623
EP  - 633
DO  - 10.1007/s00253-012-4058-4
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and O'Connor, Kevin E.",
year = "2012",
abstract = "Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate",
volume = "95",
number = "3",
pages = "623-633",
doi = "10.1007/s00253-012-4058-4"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P.,& O'Connor, K. E.. (2012). Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 95(3), 623-633.
https://doi.org/10.1007/s00253-012-4058-4

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, O'Connor KE. Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2012;95(3):623-633.
doi:10.1007/s00253-012-4058-4 .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., O'Connor, Kevin E., "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 95, no. 3 (2012):623-633,
https://doi.org/10.1007/s00253-012-4058-4 . .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - Keely, Chris M.
AU  - Blau, Werner
AU  - O'Connor, Kevin E.
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/978
AB  - The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science and Technology
T1  - Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
VL  - 42
IS  - 20
SP  - 7696
EP  - 7701
DO  - 10.1021/es801010e
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and Keely, Chris M. and Blau, Werner and O'Connor, Kevin E.",
year = "2008",
abstract = "The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science and Technology",
title = "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)",
volume = "42",
number = "20",
pages = "7696-7701",
doi = "10.1021/es801010e"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P., Keely, C. M., Blau, W.,& O'Connor, K. E.. (2008). Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology
Amer Chemical Soc, Washington., 42(20), 7696-7701.
https://doi.org/10.1021/es801010e

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, Keely CM, Blau W, O'Connor KE. Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology. 2008;42(20):7696-7701.
doi:10.1021/es801010e .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., Keely, Chris M., Blau, Werner, O'Connor, Kevin E., "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)" in Environmental Science and Technology, 42, no. 20 (2008):7696-7701,
https://doi.org/10.1021/es801010e . .