TY  - CONF
AU  - Sibinčić, Nikolina
AU  - Veličković, Luka
AU  - Minić, Simeon
AU  - Stojadinović, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6200
AB  - Both Arthrospira platensis (Spirulina) and Porphyra sp. (Nori) are algae known for their richness in vitamins, minerals, antioxidants, proteins, such as phycobiliproteins (PBPs). Тhanks to their exceptional nutritional properties they have potential to be considered as a high-quality substitute for fetal bovine serum (FBS) in cellular cultivation, which has numerous drawbacks since it can be involved in contamination development and its composition is still unclear. In this study we investigated the influence of Spirulina and Nori extracts on HEK293T cell line growth and viability in serum-reduced conditions. In DMEM/F12 medium supplemented with 0–10% FBS, the concentration-dependent effects of PBPs on cell proliferation were investigated. Cell viability and cytotoxicity were evaluated by MTT assay. During 3-day observation prior to MTT assay and MTT assay itself showed that HEK293T exhibited improvement in viability at lower PBP concentrations, while presence of higher concentrations resulted in inhibition of growth and change in morphology as a consequence of their cytotoxicity at higher concentrations. These findings suggest that PBPs have a positive outcome on cell growth at relevant doses. In general, in this study were obtained results proving the potential advantages of PBPs at lower doses on cell proliferation in serum-reduced conditions, but also HEK293T cells ability to adapt in non-standard cultivation set-up.
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Arthrospira platensis and Porphyra sp. – prospective serum- substitute in HEK293T cell culture
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6200
ER  - 

@conference{
author = "Sibinčić, Nikolina and Veličković, Luka and Minić, Simeon and Stojadinović, Marija",
year = "2023",
abstract = "Both Arthrospira platensis (Spirulina) and Porphyra sp. (Nori) are algae known for their richness in vitamins, minerals, antioxidants, proteins, such as phycobiliproteins (PBPs). Тhanks to their exceptional nutritional properties they have potential to be considered as a high-quality substitute for fetal bovine serum (FBS) in cellular cultivation, which has numerous drawbacks since it can be involved in contamination development and its composition is still unclear. In this study we investigated the influence of Spirulina and Nori extracts on HEK293T cell line growth and viability in serum-reduced conditions. In DMEM/F12 medium supplemented with 0–10% FBS, the concentration-dependent effects of PBPs on cell proliferation were investigated. Cell viability and cytotoxicity were evaluated by MTT assay. During 3-day observation prior to MTT assay and MTT assay itself showed that HEK293T exhibited improvement in viability at lower PBP concentrations, while presence of higher concentrations resulted in inhibition of growth and change in morphology as a consequence of their cytotoxicity at higher concentrations. These findings suggest that PBPs have a positive outcome on cell growth at relevant doses. In general, in this study were obtained results proving the potential advantages of PBPs at lower doses on cell proliferation in serum-reduced conditions, but also HEK293T cells ability to adapt in non-standard cultivation set-up.",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Arthrospira platensis and Porphyra sp. – prospective serum- substitute in HEK293T cell culture",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6200"
}

Sibinčić, N., Veličković, L., Minić, S.,& Stojadinović, M.. (2023). Arthrospira platensis and Porphyra sp. – prospective serum- substitute in HEK293T cell culture. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
Belgrade : Serbian Chemical Society..
https://hdl.handle.net/21.15107/rcub_cherry_6200

Sibinčić N, Veličković L, Minić S, Stojadinović M. Arthrospira platensis and Porphyra sp. – prospective serum- substitute in HEK293T cell culture. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6200 .

Sibinčić, Nikolina, Veličković, Luka, Minić, Simeon, Stojadinović, Marija, "Arthrospira platensis and Porphyra sp. – prospective serum- substitute in HEK293T cell culture" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6200 .