TY  - JOUR
AU  - Đokić, Lidija
AU  - Narančić, Tanja
AU  - Nikodinović-Runić, Jasmina
AU  - Savic, Miloje
AU  - Vasiljević, Branka
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1183
AB  - Four new Gram-positive, phenol-degrading strains were isolated from the rhizospheres of endemorelict plants Ramonda serbica and Ramonda nathaliae known to exude high amounts of phenolics in the soil. Isolates were designated Bacillus sp. PS1, Bacillus sp. PS11, Streptomyces sp. PS12, and Streptomyces sp. PN1 based on 16S rDNA sequence and biochemical analysis. In addition to their ability to tolerate and utilize high amounts of phenol of either up to 800 or up to 1,400 mg l(-1) without apparent inhibition in growth, all four strains were also able to degrade a broad range of aromatic substrates including benzene, toluene, ethylbenzene, xylenes, styrene, halogenated benzenes, and naphthalene. Isolates were able to grow in pure culture and in defined mixed culture on phenol and on the mixture of BTEX (benzene, toluene, ethylbenzene, and xylenes) compounds as a sole source of carbon and energy. Pure culture of Bacillus sp. PS11 yielded 1.5-fold higher biomass amounts in comparison to mixed culture, under all conditions. Strains successfully degraded phenol in the soil model system (2 g kg(-1)) within 6 days. Activities of phenol hydroxylase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase were detected and analyzed from the crude cell extract of the isolates. While all four strains use ortho degradation pathway, enzyme indicative of meta degradation pathway (catechol 2,3-dioxygenase) was also detected in Bacillus sp. PS11 and Streptomyces sp. PN1. Phenol degradation activities were induced 2 h after supplementation by phenol, but not by catechol. Catechol slightly inhibited activity of catechol 2,3-dioxygenase in strains PS11 and PN1.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Isolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates
VL  - 91
IS  - 4
SP  - 1227
EP  - 1238
DO  - 10.1007/s00253-011-3426-9
ER  - 

@article{
author = "Đokić, Lidija and Narančić, Tanja and Nikodinović-Runić, Jasmina and Savic, Miloje and Vasiljević, Branka",
year = "2011",
abstract = "Four new Gram-positive, phenol-degrading strains were isolated from the rhizospheres of endemorelict plants Ramonda serbica and Ramonda nathaliae known to exude high amounts of phenolics in the soil. Isolates were designated Bacillus sp. PS1, Bacillus sp. PS11, Streptomyces sp. PS12, and Streptomyces sp. PN1 based on 16S rDNA sequence and biochemical analysis. In addition to their ability to tolerate and utilize high amounts of phenol of either up to 800 or up to 1,400 mg l(-1) without apparent inhibition in growth, all four strains were also able to degrade a broad range of aromatic substrates including benzene, toluene, ethylbenzene, xylenes, styrene, halogenated benzenes, and naphthalene. Isolates were able to grow in pure culture and in defined mixed culture on phenol and on the mixture of BTEX (benzene, toluene, ethylbenzene, and xylenes) compounds as a sole source of carbon and energy. Pure culture of Bacillus sp. PS11 yielded 1.5-fold higher biomass amounts in comparison to mixed culture, under all conditions. Strains successfully degraded phenol in the soil model system (2 g kg(-1)) within 6 days. Activities of phenol hydroxylase, catechol 1,2-dioxygenase, and catechol 2,3-dioxygenase were detected and analyzed from the crude cell extract of the isolates. While all four strains use ortho degradation pathway, enzyme indicative of meta degradation pathway (catechol 2,3-dioxygenase) was also detected in Bacillus sp. PS11 and Streptomyces sp. PN1. Phenol degradation activities were induced 2 h after supplementation by phenol, but not by catechol. Catechol slightly inhibited activity of catechol 2,3-dioxygenase in strains PS11 and PN1.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Isolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates",
volume = "91",
number = "4",
pages = "1227-1238",
doi = "10.1007/s00253-011-3426-9"
}

Đokić, L., Narančić, T., Nikodinović-Runić, J., Savic, M.,& Vasiljević, B.. (2011). Isolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates. in Applied Microbiology and Biotechnology
Springer, New York., 91(4), 1227-1238.
https://doi.org/10.1007/s00253-011-3426-9

Đokić L, Narančić T, Nikodinović-Runić J, Savic M, Vasiljević B. Isolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates. in Applied Microbiology and Biotechnology. 2011;91(4):1227-1238.
doi:10.1007/s00253-011-3426-9 .

Đokić, Lidija, Narančić, Tanja, Nikodinović-Runić, Jasmina, Savic, Miloje, Vasiljević, Branka, "Isolation and characterization of four novel Gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates" in Applied Microbiology and Biotechnology, 91, no. 4 (2011):1227-1238,
https://doi.org/10.1007/s00253-011-3426-9 . .