Ivković, Branka

Link to this page

Authority KeyName Variants
888a7cd1-cafa-43a0-a48a-e84dc4016024
  • Ivković, Branka (3)
Projects

Author's Bibliography

Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum

Tubić, Biljana K.; Marković, Bojan D.; Vladimirov, Sandra S.; Ristić, Slavica M.; Ivković, Branka; Savić, Miroslav M.; Poljarević, Jelena; Sabo, Tibor

(Akademiai Kiado Rt, Budapest, 2017)

TY  - JOUR
AU  - Tubić, Biljana K.
AU  - Marković, Bojan D.
AU  - Vladimirov, Sandra S.
AU  - Ristić, Slavica M.
AU  - Ivković, Branka
AU  - Savić, Miroslav M.
AU  - Poljarević, Jelena
AU  - Sabo, Tibor
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2459
AB  - A series of new (S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate esters has shown cytotoxic activity towards human leukemic cell lines. The aim of this study was to develop and validate a bioanalytical method for quantification of (S,S)-O,O-diethyl-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochlorides (DE-EDCP) and its metabolite, substituted propanoic acid (EDCP), in mouse serum by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Structural analog, derivative of 1,3-propanediamine, was used as an internal standard (IS). Sample preparation employed protein precipitation by acetonitrile and subsequent centrifugation. Optimal UHPLC separation conditions were set to achieve simultaneous determination of both compounds in a short run time of 6 min. Additionally, the selected reaction monitoring (SRM) mode developed in this method allowed a highly sensitive, accurate, and precise identification of compounds of interest. The lower limit of quantitation (LOQ) was 1.3 ng mL(-1) for DE-EDCP and 0.3 mu g mL(-1) for EDCP. The calibration curves were linear over the concentration range of 1.3-26.7 ng mL(-1) and 0.3-6.7 mu g mL(-1) for DE-EDCP and EDCP, respectively. Precision (%CV) and accuracy (% RE) for DE-EDCP and EDCP ranged from 3.5% to 16.0% and from 1.8% to 14.4%, respectively. The validation process was performed in accordance with the regulatory guidance/guideline, and all of the obtained results met the established acceptance criteria. The newly developed and validated UHPLC-MS/MS method is rapid, sensitive, and selective, and it can be successfully applied to drug monitoring in nonclinical studies.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Chromatographica
T1  - Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum
VL  - 29
IS  - 2
SP  - 235
EP  - 252
DO  - 10.1556/1326.2017.29.2.7
ER  - 
@article{
author = "Tubić, Biljana K. and Marković, Bojan D. and Vladimirov, Sandra S. and Ristić, Slavica M. and Ivković, Branka and Savić, Miroslav M. and Poljarević, Jelena and Sabo, Tibor",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2459",
abstract = "A series of new (S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate esters has shown cytotoxic activity towards human leukemic cell lines. The aim of this study was to develop and validate a bioanalytical method for quantification of (S,S)-O,O-diethyl-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochlorides (DE-EDCP) and its metabolite, substituted propanoic acid (EDCP), in mouse serum by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Structural analog, derivative of 1,3-propanediamine, was used as an internal standard (IS). Sample preparation employed protein precipitation by acetonitrile and subsequent centrifugation. Optimal UHPLC separation conditions were set to achieve simultaneous determination of both compounds in a short run time of 6 min. Additionally, the selected reaction monitoring (SRM) mode developed in this method allowed a highly sensitive, accurate, and precise identification of compounds of interest. The lower limit of quantitation (LOQ) was 1.3 ng mL(-1) for DE-EDCP and 0.3 mu g mL(-1) for EDCP. The calibration curves were linear over the concentration range of 1.3-26.7 ng mL(-1) and 0.3-6.7 mu g mL(-1) for DE-EDCP and EDCP, respectively. Precision (%CV) and accuracy (% RE) for DE-EDCP and EDCP ranged from 3.5% to 16.0% and from 1.8% to 14.4%, respectively. The validation process was performed in accordance with the regulatory guidance/guideline, and all of the obtained results met the established acceptance criteria. The newly developed and validated UHPLC-MS/MS method is rapid, sensitive, and selective, and it can be successfully applied to drug monitoring in nonclinical studies.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Chromatographica",
title = "Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum",
volume = "29",
number = "2",
pages = "235-252",
doi = "10.1556/1326.2017.29.2.7"
}
Tubić, B. K., Marković, B. D., Vladimirov, S. S., Ristić, S. M., Ivković, B., Savić, M. M., Poljarević, J.,& Sabo, T. (2017). Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum.
Acta Chromatographica
Akademiai Kiado Rt, Budapest., 29(2), 235-252.
https://doi.org/10.1556/1326.2017.29.2.7
Tubić BK, Marković BD, Vladimirov SS, Ristić SM, Ivković B, Savić MM, Poljarević J, Sabo T. Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum. Acta Chromatographica. 2017;29(2):235-252
Tubić Biljana K., Marković Bojan D., Vladimirov Sandra S., Ristić Slavica M., Ivković Branka, Savić Miroslav M., Poljarević Jelena, Sabo Tibor, "Highly Sensitive UHPLC-MS/MS Method for Quantification of Ethylenediamine-N,N '-di-2-(3-cyclohexyl) Propanoic Acid Derivatives in Mouse Serum" Acta Chromatographica, 29, no. 2 (2017):235-252,
https://doi.org/10.1556/1326.2017.29.2.7 .
3
5
5

Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010

Jovanović, Predrag M.; Jeremić, Sanja; Đokić, Lidija; Savić, Vladimir; Radivojević, Jelena; Maslak, Veselin; Ivković, Branka; Vasiljević, Branka; Nikodinović-Runić, Jasmina

(Elsevier Science Inc, New York, 2014)

TY  - BOOK
AU  - Jovanović, Predrag M.
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Savić, Vladimir
AU  - Radivojević, Jelena
AU  - Maslak, Veselin
AU  - Ivković, Branka
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3744
PB  - Elsevier Science Inc, New York
T2  - Enzyme and Microbial Technology
T1  - Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010
ER  - 
@book{
author = "Jovanović, Predrag M. and Jeremić, Sanja and Đokić, Lidija and Savić, Vladimir and Radivojević, Jelena and Maslak, Veselin and Ivković, Branka and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3744",
publisher = "Elsevier Science Inc, New York",
journal = "Enzyme and Microbial Technology",
title = "Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010"
}
Jovanović, P. M., Jeremić, S., Đokić, L., Savić, V., Radivojević, J., Maslak, V., Ivković, B., Vasiljević, B.,& Nikodinović-Runić, J. (2014). Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010.
Enzyme and Microbial Technology
Elsevier Science Inc, New York..
Jovanović PM, Jeremić S, Đokić L, Savić V, Radivojević J, Maslak V, Ivković B, Vasiljević B, Nikodinović-Runić J. Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010. Enzyme and Microbial Technology. 2014;
Jovanović Predrag M., Jeremić Sanja, Đokić Lidija, Savić Vladimir, Radivojević Jelena, Maslak Veselin, Ivković Branka, Vasiljević Branka, Nikodinović-Runić Jasmina, "Supplementary data for the article: Jovanovic, P.; Jeremic, S.; Djokic, L.; Savic, V.; Radivojevic, J.; Maslak, V.; Ivkovic, B.; Vasiljevic, B.; Nikodinovic-Runic, J. Chemoselective Biocatalytic Reduction of Conjugated Nitroalkenes: New Application for an Escherichia Coli BL21(DE3) Expression Strain. Enzyme Microb. Technol. 2014, 60, 16–23. https://doi.org/10.1016/j.enzmictec.2014.03.010" Enzyme and Microbial Technology (2014)

Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain

Jovanović, Predrag M.; Jeremić, Sanja; Đokić, Lidija; Savić, Vladimir; Radivojević, Jelena; Maslak, Veselin; Ivković, Branka; Vasiljević, Branka; Nikodinović-Runić, Jasmina

(Elsevier Science Inc, New York, 2014)

TY  - JOUR
AU  - Jovanović, Predrag M.
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Savić, Vladimir
AU  - Radivojević, Jelena
AU  - Maslak, Veselin
AU  - Ivković, Branka
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1786
AB  - Chemoselective reduction of activated carbon-carbon double bond in conjugated nitroalkenes was achieved using Escherichia coli BL21(DE3) whole cells. Nine different substrates have been used furnishing the reduced products in moderate to good yields. 1-Nitro-4-phenyl-1,3-butadiene and (2-nitro-1-propenyl)benzene were successfully biotransformed with corresponding product yields of 54% and 45% respectively. Using this simple and environmentally friendly system 2-(2-nitropropyl)pyridine and 2-(2-nitropropyl)naphthalene were synthesized and characterized for the first time. High substrate conversion efficiency was coupled with low enantioselectivity, however 29% enantiomeric excess was detected in the case of 2-(2-nitropropyl)pyridine. It was shown that electronic properties of the aromatic ring, which affected polarity of the double bond, were not highly influential factors in the reduction process, but the presence of the nitro functionality was essential for the reaction to proceed. 1-Phenyl-4-nitro-1,3-butadiene could not be biotransformed by whole cells of Pseudomonas putida KT2440 or Bacillus subtilis 168 while it was successfully reduced by E. coli DH5 alpha but with lower efficiency in comparison to E. coli BL21(DE3). Knockout mutant affected in nemA gene coding for N-ethylmaleimide reductase (BL21 Delta nemA) could still catalyze bioreductions suggesting multiple active reductases within E. coli BL21(DE3) biocatalyst. The described biocatalytic reduction of substituted nitroalkenes provides an efficient route for the preparation of the corresponding nitroalkanes and introduces the new application of the strain traditionally utilized for recombinant protein expression. (C) 2014 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Enzyme and Microbial Technology
T1  - Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain
VL  - 60
SP  - 16
EP  - 23
DO  - 10.1016/j.enzmictec.2014.03.010
ER  - 
@article{
author = "Jovanović, Predrag M. and Jeremić, Sanja and Đokić, Lidija and Savić, Vladimir and Radivojević, Jelena and Maslak, Veselin and Ivković, Branka and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1786",
abstract = "Chemoselective reduction of activated carbon-carbon double bond in conjugated nitroalkenes was achieved using Escherichia coli BL21(DE3) whole cells. Nine different substrates have been used furnishing the reduced products in moderate to good yields. 1-Nitro-4-phenyl-1,3-butadiene and (2-nitro-1-propenyl)benzene were successfully biotransformed with corresponding product yields of 54% and 45% respectively. Using this simple and environmentally friendly system 2-(2-nitropropyl)pyridine and 2-(2-nitropropyl)naphthalene were synthesized and characterized for the first time. High substrate conversion efficiency was coupled with low enantioselectivity, however 29% enantiomeric excess was detected in the case of 2-(2-nitropropyl)pyridine. It was shown that electronic properties of the aromatic ring, which affected polarity of the double bond, were not highly influential factors in the reduction process, but the presence of the nitro functionality was essential for the reaction to proceed. 1-Phenyl-4-nitro-1,3-butadiene could not be biotransformed by whole cells of Pseudomonas putida KT2440 or Bacillus subtilis 168 while it was successfully reduced by E. coli DH5 alpha but with lower efficiency in comparison to E. coli BL21(DE3). Knockout mutant affected in nemA gene coding for N-ethylmaleimide reductase (BL21 Delta nemA) could still catalyze bioreductions suggesting multiple active reductases within E. coli BL21(DE3) biocatalyst. The described biocatalytic reduction of substituted nitroalkenes provides an efficient route for the preparation of the corresponding nitroalkanes and introduces the new application of the strain traditionally utilized for recombinant protein expression. (C) 2014 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Enzyme and Microbial Technology",
title = "Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain",
volume = "60",
pages = "16-23",
doi = "10.1016/j.enzmictec.2014.03.010"
}
Jovanović, P. M., Jeremić, S., Đokić, L., Savić, V., Radivojević, J., Maslak, V., Ivković, B., Vasiljević, B.,& Nikodinović-Runić, J. (2014). Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain.
Enzyme and Microbial Technology
Elsevier Science Inc, New York., 60, 16-23.
https://doi.org/10.1016/j.enzmictec.2014.03.010
Jovanović PM, Jeremić S, Đokić L, Savić V, Radivojević J, Maslak V, Ivković B, Vasiljević B, Nikodinović-Runić J. Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain. Enzyme and Microbial Technology. 2014;60:16-23
Jovanović Predrag M., Jeremić Sanja, Đokić Lidija, Savić Vladimir, Radivojević Jelena, Maslak Veselin, Ivković Branka, Vasiljević Branka, Nikodinović-Runić Jasmina, "Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain" Enzyme and Microbial Technology, 60 (2014):16-23,
https://doi.org/10.1016/j.enzmictec.2014.03.010 .
4
4
4