TY  - JOUR
AU  - Radotić, Ksenija
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Natić, Maja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6326
AB  - Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.
PB  - MDPI
T2  - Foods
T1  - Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach
VL  - 12
IS  - 16
SP  - 3023
DO  - 10.3390/foods12163023
ER  - 

@article{
author = "Radotić, Ksenija and Stanković, Mira and Bartolić, Dragana and Natić, Maja",
year = "2023",
abstract = "Food is a complex matrix of proteins, fats, minerals, vitamins, and other components. Various analytical methods are currently used for food testing. However, most of the used methods require sample preprocessing and expensive chemicals. New analytical methods are needed for quick and economic measurement of food quality and safety. Fluorescence spectroscopy is a simple and quick method to measure food quality, without sample preprocessing. This technique has been developed for food samples due to the application of a front-face measuring setup. Fluorescent compounds–fluorophores in the food samples are highly sensitive to their environment. Information about molecular structure and changes in food samples is obtained by the measurement of excitation–emission matrices of the endogenous fluorophores and by applying multivariate chemometric tools. Synchronous fluorescence spectroscopy is an advantageous screening mode used in food analysis. The fluorescent markers in food are amino acids tryptophan and tyrosine; the structural proteins collagen and elastin; the enzymes and co-enzymes NADH and FAD; vitamins; lipids; porphyrins; and mycotoxins in certain food types. The review provides information on the principles of the fluorescence measurements of food samples and the advantages of this method over the others. An analysis of the fluorescence spectroscopy applications in screening the various food types is provided.",
publisher = "MDPI",
journal = "Foods",
title = "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach",
volume = "12",
number = "16",
pages = "3023",
doi = "10.3390/foods12163023"
}

Radotić, K., Stanković, M., Bartolić, D.,& Natić, M.. (2023). Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods
MDPI., 12(16), 3023.
https://doi.org/10.3390/foods12163023

Radotić K, Stanković M, Bartolić D, Natić M. Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach. in Foods. 2023;12(16):3023.
doi:10.3390/foods12163023 .

Radotić, Ksenija, Stanković, Mira, Bartolić, Dragana, Natić, Maja, "Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach" in Foods, 12, no. 16 (2023):3023,
https://doi.org/10.3390/foods12163023 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baošić, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5268
AB  - Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study
VL  - 102
IS  - 6
SP  - 2500
EP  - 2505
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baošić, Rada and Radotić, Ksenija",
year = "2022",
abstract = "Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study",
volume = "102",
number = "6",
pages = "2500-2505",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baošić, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baošić R, Radotić K. Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baošić, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Maksimović, Jelena D.
AU  - Stanković, Mira
AU  - Krstović, Saša
AU  - Baošić, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3949
AB  - BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship
VL  - 100
IS  - 7
SP  - 2905
EP  - 2910
DO  - 10.1002/jsfa.10317
ER  - 

@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Maksimović, Jelena D. and Stanković, Mira and Krstović, Saša and Baošić, Rada and Radotić, Ksenija",
year = "2020",
abstract = "BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship",
volume = "100",
number = "7",
pages = "2905-2910",
doi = "10.1002/jsfa.10317"
}

Bartolić, D., Maksimović, V., Maksimović, J. D., Stanković, M., Krstović, S., Baošić, R.,& Radotić, K.. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship. in Journal of the Science of Food and Agriculture
Wiley., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317

Bartolić D, Maksimović V, Maksimović JD, Stanković M, Krstović S, Baošić R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship. in Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910.
doi:10.1002/jsfa.10317 .

Bartolić, Dragana, Maksimović, Vuk, Maksimović, Jelena D., Stanković, Mira, Krstović, Saša, Baošić, Rada, Radotić, Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship" in Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 . .