TY  - JOUR
AU  - Dorm, Bruna Carolina
AU  - Rosas Costa Iemma, Mônica
AU  - Domingos Neto, Benedito
AU  - Lopes Francisco, Rauany Cristina
AU  - Dinić, Ivana
AU  - Ignjatović, Nenad
AU  - Marković, Smilja
AU  - Vuković, Marina
AU  - Škapin, Srečo
AU  - Trovatti, Eliane
AU  - Mančić, Lidija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5814
AB  - Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.
PB  - MDPI
T2  - Life
T1  - Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles
VL  - 13
IS  - 1
SP  - 116
DO  - 10.3390/life13010116
ER  - 

@article{
author = "Dorm, Bruna Carolina and Rosas Costa Iemma, Mônica and Domingos Neto, Benedito and Lopes Francisco, Rauany Cristina and Dinić, Ivana and Ignjatović, Nenad and Marković, Smilja and Vuković, Marina and Škapin, Srečo and Trovatti, Eliane and Mančić, Lidija",
year = "2023",
abstract = "Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.",
publisher = "MDPI",
journal = "Life",
title = "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles",
volume = "13",
number = "1",
pages = "116",
doi = "10.3390/life13010116"
}

Dorm, B. C., Rosas Costa Iemma, M., Domingos Neto, B., Lopes Francisco, R. C., Dinić, I., Ignjatović, N., Marković, S., Vuković, M., Škapin, S., Trovatti, E.,& Mančić, L.. (2023). Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life
MDPI., 13(1), 116.
https://doi.org/10.3390/life13010116

Dorm BC, Rosas Costa Iemma M, Domingos Neto B, Lopes Francisco RC, Dinić I, Ignjatović N, Marković S, Vuković M, Škapin S, Trovatti E, Mančić L. Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life. 2023;13(1):116.
doi:10.3390/life13010116 .

Dorm, Bruna Carolina, Rosas Costa Iemma, Mônica, Domingos Neto, Benedito, Lopes Francisco, Rauany Cristina, Dinić, Ivana, Ignjatović, Nenad, Marković, Smilja, Vuković, Marina, Škapin, Srečo, Trovatti, Eliane, Mančić, Lidija, "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles" in Life, 13, no. 1 (2023):116,
https://doi.org/10.3390/life13010116 . .