TY  - JOUR
AU  - Jović, Marko D.
AU  - Agatonovic-Kustrin, Snezana
AU  - Ristivojević, Petar 
AU  - Trifković, Jelena 
AU  - Morton, David W.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6304
AB  - Natural products and their analogues have contributed significantly to treatment options, especially for anti-inflammatory and infectious diseases. Thus, the primary objective of this work was to compare the bioactivity profiles of selected medicinal plants that are historically used in folk medicine to treat inflammation and infections in the body. Chemical HPTLC fingerprinting was used to assess antioxidant, phenolic and flavonoid content, while bioassay-guided HPTLC was used to detect compounds with the highest antibacterial and anti-inflammatory activities. The results of this study showed that green tea leaf, walnut leaf, St. John’s wort herb, wild thyme herb, European goldenrod herb, chamomile flower, and immortelle flower extracts were strong radical scavengers. Green tea and nettle extracts were the most active extracts against E. coli, while calendula flower extract showed significant potency against S. aureus. Furthermore, green tea, greater celandine, and fumitory extracts exhibited pronounced potential in suppressing COX-1 activity. The bioactive compounds from the green tea extract, as the most bioactive, were isolated by preparative thin-layer chromatography and characterized with their FTIR spectra. Although earlier studies have related green tea’s anti-inflammatory properties to the presence of catechins, particularly epigallocatechin-3-gallate, the FTIR spectrum of the compound from the most intense bioactive zone showed the strongest anti-inflammatory activity can be attributed to amino acids and heterocyclic compounds. As expected, antibacterial activity in extracts was related to fatty acids and monoglycerides.
PB  - MDPI
T2  - Molecules
T1  - Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography
VL  - 28
IS  - 21
SP  - 7346
DO  - 10.3390/molecules28217346
ER  - 

@article{
author = "Jović, Marko D. and Agatonovic-Kustrin, Snezana and Ristivojević, Petar  and Trifković, Jelena  and Morton, David W.",
year = "2023",
abstract = "Natural products and their analogues have contributed significantly to treatment options, especially for anti-inflammatory and infectious diseases. Thus, the primary objective of this work was to compare the bioactivity profiles of selected medicinal plants that are historically used in folk medicine to treat inflammation and infections in the body. Chemical HPTLC fingerprinting was used to assess antioxidant, phenolic and flavonoid content, while bioassay-guided HPTLC was used to detect compounds with the highest antibacterial and anti-inflammatory activities. The results of this study showed that green tea leaf, walnut leaf, St. John’s wort herb, wild thyme herb, European goldenrod herb, chamomile flower, and immortelle flower extracts were strong radical scavengers. Green tea and nettle extracts were the most active extracts against E. coli, while calendula flower extract showed significant potency against S. aureus. Furthermore, green tea, greater celandine, and fumitory extracts exhibited pronounced potential in suppressing COX-1 activity. The bioactive compounds from the green tea extract, as the most bioactive, were isolated by preparative thin-layer chromatography and characterized with their FTIR spectra. Although earlier studies have related green tea’s anti-inflammatory properties to the presence of catechins, particularly epigallocatechin-3-gallate, the FTIR spectrum of the compound from the most intense bioactive zone showed the strongest anti-inflammatory activity can be attributed to amino acids and heterocyclic compounds. As expected, antibacterial activity in extracts was related to fatty acids and monoglycerides.",
publisher = "MDPI",
journal = "Molecules",
title = "Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography",
volume = "28",
number = "21",
pages = "7346",
doi = "10.3390/molecules28217346"
}

Jović, M. D., Agatonovic-Kustrin, S., Ristivojević, P., Trifković, J.,& Morton, D. W.. (2023). Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography. in Molecules
MDPI., 28(21), 7346.
https://doi.org/10.3390/molecules28217346

Jović MD, Agatonovic-Kustrin S, Ristivojević P, Trifković J, Morton DW. Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography. in Molecules. 2023;28(21):7346.
doi:10.3390/molecules28217346 .

Jović, Marko D., Agatonovic-Kustrin, Snezana, Ristivojević, Petar , Trifković, Jelena , Morton, David W., "Bioassay-Guided Assessment of Antioxidative, Anti-Inflammatory and Antimicrobial Activities of Extracts from Medicinal Plants via High-Performance Thin-Layer Chromatography" in Molecules, 28, no. 21 (2023):7346,
https://doi.org/10.3390/molecules28217346 . .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Jović, Marko D.
AU  - Dimkić, Ivica Z.
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
AU  - Trifković, Jelena 
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6220
AB  - Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.
C3  - XXII Congress EuroFoodChem, Book of abstracts
T1  - Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6220
ER  - 

@conference{
author = "Lazović, Mila Č. and Jović, Marko D. and Dimkić, Ivica Z. and Milojković-Opsenica, Dušanka and Ristivojević, Petar and Trifković, Jelena ",
year = "2023",
abstract = "Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.",
journal = "XXII Congress EuroFoodChem, Book of abstracts",
title = "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6220"
}

Lazović, M. Č., Jović, M. D., Dimkić, I. Z., Milojković-Opsenica, D., Ristivojević, P.,& Trifković, J.. (2023). Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6220

Lazović MČ, Jović MD, Dimkić IZ, Milojković-Opsenica D, Ristivojević P, Trifković J. Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

Lazović, Mila Č., Jović, Marko D., Dimkić, Ivica Z., Milojković-Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena , "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries" in XXII Congress EuroFoodChem, Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Krstić, Đurđa D.
AU  - Jović, Marko D.
AU  - Trifković, Jelena 
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6217
AB  - Blackberry wine is produced by a yeast fermentation of natural saccharides present in blackberry juice. This wine is traditionally consumed in Balkans, mostly as a dessert wine, but in recent years it is gaining more attention due to unique flavour and potential health benefits. Blackberries are a natural source of many minerals and bioactive phytochemicals such as vitamins, folic acid, anthocyanins, and phenolics1,2. Considering biological potential of blackberries and their products aim of this study was to evaluate quality of Serbian blackberry wines. This quality assessment was achieved through determination of: (a) the phenolic profile by high-performance thin layer chromatography (HPTLC); (b) total phenolic (TPC) and anthocyanin content (TAC); (c) in vitro antioxidant activity by DPPH and ABTS radical scavenging assays and total antioxidant capacity (d) in vitro antimicrobial activity by the agar diffusion test; and (e) the content of macro, micro and toxic elements by inductively coupled plasma mass spectrometry (ICP-MS). Twelve blackberry wines from different geographical regions were tested indicating various phenolic profiles and great antioxidant and antimicrobial activity. Strong positive correlation was confirmed between TPC and antioxidant activity determined by DPPH and ABTS assays. Additionally, high levels of antibacterial activity were shown against all six tested strains, but primarily against Staphylococcus aureus and Micrococcus lysodeikticus. Samples were rich in macroelements, such as Ca, K, Mg, Na, P and Zn, and microelements Cu and Fe, while Mn was present in higher concentration only in one sample. Toxic metals Cd and Pb were present in low concertation in all samples. These results indicate great quality of Serbian blackberry wines concerning minerals, phenolics, antioxidant and antimicrobial activity, which support its health potential.
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6217
ER  - 

@conference{
author = "Lazović, Mila Č. and Krstić, Đurđa D. and Jović, Marko D. and Trifković, Jelena ",
year = "2023",
abstract = "Blackberry wine is produced by a yeast fermentation of natural saccharides present in blackberry juice. This wine is traditionally consumed in Balkans, mostly as a dessert wine, but in recent years it is gaining more attention due to unique flavour and potential health benefits. Blackberries are a natural source of many minerals and bioactive phytochemicals such as vitamins, folic acid, anthocyanins, and phenolics1,2. Considering biological potential of blackberries and their products aim of this study was to evaluate quality of Serbian blackberry wines. This quality assessment was achieved through determination of: (a) the phenolic profile by high-performance thin layer chromatography (HPTLC); (b) total phenolic (TPC) and anthocyanin content (TAC); (c) in vitro antioxidant activity by DPPH and ABTS radical scavenging assays and total antioxidant capacity (d) in vitro antimicrobial activity by the agar diffusion test; and (e) the content of macro, micro and toxic elements by inductively coupled plasma mass spectrometry (ICP-MS). Twelve blackberry wines from different geographical regions were tested indicating various phenolic profiles and great antioxidant and antimicrobial activity. Strong positive correlation was confirmed between TPC and antioxidant activity determined by DPPH and ABTS assays. Additionally, high levels of antibacterial activity were shown against all six tested strains, but primarily against Staphylococcus aureus and Micrococcus lysodeikticus. Samples were rich in macroelements, such as Ca, K, Mg, Na, P and Zn, and microelements Cu and Fe, while Mn was present in higher concentration only in one sample. Toxic metals Cd and Pb were present in low concertation in all samples. These results indicate great quality of Serbian blackberry wines concerning minerals, phenolics, antioxidant and antimicrobial activity, which support its health potential.",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6217"
}

Lazović, M. Č., Krstić, Đ. D., Jović, M. D.,& Trifković, J.. (2023). Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science.
https://hdl.handle.net/21.15107/rcub_cherry_6217

Lazović MČ, Krstić ĐD, Jović MD, Trifković J. Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6217 .

Lazović, Mila Č., Krstić, Đurđa D., Jović, Marko D., Trifković, Jelena , "Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6217 .

TY  - CONF
AU  - Trifković, Jelena
AU  - Jović, Marko D.
AU  - Živković-Radovanović, Vukosava
AU  - Andrić, Filip
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5229
AB  - The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.
C3  - 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia
T1  - Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5229
ER  - 

@conference{
author = "Trifković, Jelena and Jović, Marko D. and Živković-Radovanović, Vukosava and Andrić, Filip and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.",
journal = "27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia",
title = "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5229"
}

Trifković, J., Jović, M. D., Živković-Radovanović, V., Andrić, F., Ristivojević, P.,& Milojković-Opsenica, D.. (2021). Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia.
https://hdl.handle.net/21.15107/rcub_cherry_5229

Trifković J, Jović MD, Živković-Radovanović V, Andrić F, Ristivojević P, Milojković-Opsenica D. Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_5229 .

Trifković, Jelena, Jović, Marko D., Živković-Radovanović, Vukosava, Andrić, Filip, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare" in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia (2021),
https://hdl.handle.net/21.15107/rcub_cherry_5229 .

TY  - CONF
AU  - Trifković, Jelena
AU  - Jović, Marko D.
AU  - Živković-Radovanović, Vukosava
AU  - Andrić, Filip
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5263
AB  - The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.
C3  - 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia
T1  - Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5263
ER  - 

@conference{
author = "Trifković, Jelena and Jović, Marko D. and Živković-Radovanović, Vukosava and Andrić, Filip and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "The guarantee of food safety and quality along the food chain has become an important issue which challenges the worldwide and attracts the extensive attention. In order to ensure food safety and quality, efficient analytical methodologies must be applied in the detection of food authenticity, origin, product quality control, food microbiology, genetically modified ingredients, pesticide residues, allergens and toxins.[1] The aim of this study was to develop a green, simple, and high throughput procedure for the quality assessment of food supplements using a high-performance thin-layer chromatography (HPTLC)/bioautography with multivariate statistics. Bioautography assays were developed for the identification of constituents with radical scavenging (DPPH assay), antimicrobial (Bacillus subtilis, Escherichia coli, Micrococus luteus), and enzymes (alpha-amylase) activities of different oregano samples (Figure 1). Further, the combination of chemical screening/bioprofiling and chemometrics was used for identification of target, most potent compounds and additionally, search of the most potent oregano samples. Due to several advantages such as minimum requirements for sample preparation and minimal amount of investigated sample, possibility of selection of eco-friendly and harmless organic solvents, in situ identification of target compounds, multi detection of investigated compounds, and low solvent consumption (200-500 μL per sample) planar chromatography/bioautography is recognized as green technique. Methodology suggested in this study confirmed its possible application in quality control of food supplements.",
journal = "27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia",
title = "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5263"
}

Trifković, J., Jović, M. D., Živković-Radovanović, V., Andrić, F., Ristivojević, P.,& Milojković-Opsenica, D.. (2021). Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia.
https://hdl.handle.net/21.15107/rcub_cherry_5263

Trifković J, Jović MD, Živković-Radovanović V, Andrić F, Ristivojević P, Milojković-Opsenica D. Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare. in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_5263 .

Trifković, Jelena, Jović, Marko D., Živković-Radovanović, Vukosava, Andrić, Filip, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Statistical analysis based green planar chromatographic methodology for quality control of food supplements: case study on Origanum vulgare" in 27th Croatian Meeting of Chemists and Chemical Engineers, 5–8 October 2021, Veli Lošinj, Croatia (2021),
https://hdl.handle.net/21.15107/rcub_cherry_5263 .

TY  - THES
AU  - Jović, Marko D.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4468
AB  - Bioautografija predstavlja brzu i praktičnu metodu za detekciju biološki aktivnih jedinjenja direktno na hromatogramu dobijenom primenom tankoslojne hromatografije (TLC). Bioautografski eseji se najčešće izvode na mikroorganizmima, enzimima i radikalskim agensima. TLC se može direktno ili indirektno sprezati sa spektroskopskim metodama u cilju dobijanja strukturnih informacija o razdvojenim, odnosno aktivnim komponentama. Još jedan od efikasnih načina prikupljanja podataka sa TLC-a jeste obrada slike hromatograma u odgovarajućim softverima. Hemometrijske metode, poput analize glavnih komponenata (PCA) ili analize razlike rangova SRD, imaju značajnu ulogu u analizi tih podataka, što omogućava izvođenje objektivnih zaključaka. Cilj ovog rada je optimizacija eksperimentalnih uslova za izvođenje bioautografije na bakterijama, gljivicama, enzimama i radikalskim agensima i definisanje protokola izvođenja ovih eseja. Sva ispitivanja rađena su na primeru ekstrakata origana (Origanum vulgre L.) čiji hemijski profili su dobijeni primenom prethodno optimizovanih uslova u visoko-efikasnoj tankoslojnoj hromatografiji (HPTLC). Bioautografski eseji i hemijski profili ukazali su na prisustvo većeg broja aktivnih komponenata, ali su uočljive i razlike između samih uzorka i korišćenih ekstrakcionih sredstava. Obradom slika hromatograma i bioautograma, i naknadnom analizom prikupljenih podataka primenom SRD i PCA, omogućeno je definisanje najboljeg ekstrakcionog sredstva i najkvalitetnijeg uzorka origana u pogledu sadržaja aktivnih jedinjenja. Snimljeni su NMR spektri prikupljenih ekstrakata, pri čemu je u svim uzorcima utvrđeno prisustvo timola i karvakrola, jedinjenja karakteristična za origano kao vrstu. Ovakvi eksperimenti su pokazali veliki značaj hemijskog profilisanja i bioeseja u cilju provere autentičnosti proizvoda i istakli veliki potencijal bioautografskih metoda u razvoju novih lekova.
T1  - Tankoslojna hromatografija sa biološkim i biohemijskim metodama derivatizacije
SP  - 7
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4468
ER  - 

@mastersthesis{
author = "Jović, Marko D.",
year = "2021",
abstract = "Bioautografija predstavlja brzu i praktičnu metodu za detekciju biološki aktivnih jedinjenja direktno na hromatogramu dobijenom primenom tankoslojne hromatografije (TLC). Bioautografski eseji se najčešće izvode na mikroorganizmima, enzimima i radikalskim agensima. TLC se može direktno ili indirektno sprezati sa spektroskopskim metodama u cilju dobijanja strukturnih informacija o razdvojenim, odnosno aktivnim komponentama. Još jedan od efikasnih načina prikupljanja podataka sa TLC-a jeste obrada slike hromatograma u odgovarajućim softverima. Hemometrijske metode, poput analize glavnih komponenata (PCA) ili analize razlike rangova SRD, imaju značajnu ulogu u analizi tih podataka, što omogućava izvođenje objektivnih zaključaka. Cilj ovog rada je optimizacija eksperimentalnih uslova za izvođenje bioautografije na bakterijama, gljivicama, enzimama i radikalskim agensima i definisanje protokola izvođenja ovih eseja. Sva ispitivanja rađena su na primeru ekstrakata origana (Origanum vulgre L.) čiji hemijski profili su dobijeni primenom prethodno optimizovanih uslova u visoko-efikasnoj tankoslojnoj hromatografiji (HPTLC). Bioautografski eseji i hemijski profili ukazali su na prisustvo većeg broja aktivnih komponenata, ali su uočljive i razlike između samih uzorka i korišćenih ekstrakcionih sredstava. Obradom slika hromatograma i bioautograma, i naknadnom analizom prikupljenih podataka primenom SRD i PCA, omogućeno je definisanje najboljeg ekstrakcionog sredstva i najkvalitetnijeg uzorka origana u pogledu sadržaja aktivnih jedinjenja. Snimljeni su NMR spektri prikupljenih ekstrakata, pri čemu je u svim uzorcima utvrđeno prisustvo timola i karvakrola, jedinjenja karakteristična za origano kao vrstu. Ovakvi eksperimenti su pokazali veliki značaj hemijskog profilisanja i bioeseja u cilju provere autentičnosti proizvoda i istakli veliki potencijal bioautografskih metoda u razvoju novih lekova.",
title = "Tankoslojna hromatografija sa biološkim i biohemijskim metodama derivatizacije",
pages = "7-60",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4468"
}

Jović, M. D.. (2021). Tankoslojna hromatografija sa biološkim i biohemijskim metodama derivatizacije. , 7-60.
https://hdl.handle.net/21.15107/rcub_cherry_4468

Jović MD. Tankoslojna hromatografija sa biološkim i biohemijskim metodama derivatizacije. 2021;:7-60.
https://hdl.handle.net/21.15107/rcub_cherry_4468 .

Jović, Marko D., "Tankoslojna hromatografija sa biološkim i biohemijskim metodama derivatizacije" (2021):7-60,
https://hdl.handle.net/21.15107/rcub_cherry_4468 .

TY  - THES
AU  - Jović, Marko D.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4010
AB  - U ovom radu vršena je ekstrakcija sekundarnih metabolita uzoraka origana (Origanum vulgare) uzetih od različitih proizvođača primenom različitih organskih rastvarača u cilju procene najboljeg ekstrakcionog sredstva. Komponente ekstakata su razdvajane primenom visoko-efikasne tankoslojne hromatografije (HPTLC) u cilju određivanja hemijskog profila, kao i bioautografija na B. subtilis-u.
T1  - Detekcija antimikrobne aktivnosti, na primeru ekstrakata origana, primenom visoko-efikasne tankoslojne hromatografije – direktne bioautografije
SP  - 6
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4010
ER  - 

@misc{
author = "Jović, Marko D.",
year = "2020",
abstract = "U ovom radu vršena je ekstrakcija sekundarnih metabolita uzoraka origana (Origanum vulgare) uzetih od različitih proizvođača primenom različitih organskih rastvarača u cilju procene najboljeg ekstrakcionog sredstva. Komponente ekstakata su razdvajane primenom visoko-efikasne tankoslojne hromatografije (HPTLC) u cilju određivanja hemijskog profila, kao i bioautografija na B. subtilis-u.",
title = "Detekcija antimikrobne aktivnosti, na primeru ekstrakata origana, primenom visoko-efikasne tankoslojne hromatografije – direktne bioautografije",
pages = "6-51",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4010"
}

Jović, M. D.. (2020). Detekcija antimikrobne aktivnosti, na primeru ekstrakata origana, primenom visoko-efikasne tankoslojne hromatografije – direktne bioautografije. , 6-51.
https://hdl.handle.net/21.15107/rcub_cherry_4010

Jović MD. Detekcija antimikrobne aktivnosti, na primeru ekstrakata origana, primenom visoko-efikasne tankoslojne hromatografije – direktne bioautografije. 2020;:6-51.
https://hdl.handle.net/21.15107/rcub_cherry_4010 .

Jović, Marko D., "Detekcija antimikrobne aktivnosti, na primeru ekstrakata origana, primenom visoko-efikasne tankoslojne hromatografije – direktne bioautografije" (2020):6-51,
https://hdl.handle.net/21.15107/rcub_cherry_4010 .