TY  - JOUR
AU  - Jovičić-Petrović, Jelena
AU  - Jeremić, Sanja
AU  - Vučković, Ivan M.
AU  - Vojnović, Sandra
AU  - Bulajic, Aleksandra
AU  - Raicevic, Vera
AU  - Nikodinović-Runić, Jasmina
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2264
AB  - Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Aspergillus Piperis A/5 from  Plum-Distilling Waste Compost Produces a Complex of Antifungal Metabolites Active Against the Phytopathogen Pythium Aphanidermatum
VL  - 68
IS  - 2
SP  - 279
EP  - 289
DO  - 10.2298/ABS150602016J
ER  - 

@article{
author = "Jovičić-Petrović, Jelena and Jeremić, Sanja and Vučković, Ivan M. and Vojnović, Sandra and Bulajic, Aleksandra and Raicevic, Vera and Nikodinović-Runić, Jasmina",
year = "2016",
abstract = "Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, beta-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/ 5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Aspergillus Piperis A/5 from  Plum-Distilling Waste Compost Produces a Complex of Antifungal Metabolites Active Against the Phytopathogen Pythium Aphanidermatum",
volume = "68",
number = "2",
pages = "279-289",
doi = "10.2298/ABS150602016J"
}

Jovičić-Petrović, J., Jeremić, S., Vučković, I. M., Vojnović, S., Bulajic, A., Raicevic, V.,& Nikodinović-Runić, J.. (2016). Aspergillus Piperis A/5 from  Plum-Distilling Waste Compost Produces a Complex of Antifungal Metabolites Active Against the Phytopathogen Pythium Aphanidermatum. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 68(2), 279-289.
https://doi.org/10.2298/ABS150602016J

Jovičić-Petrović J, Jeremić S, Vučković IM, Vojnović S, Bulajic A, Raicevic V, Nikodinović-Runić J. Aspergillus Piperis A/5 from  Plum-Distilling Waste Compost Produces a Complex of Antifungal Metabolites Active Against the Phytopathogen Pythium Aphanidermatum. in Archives of biological sciences. 2016;68(2):279-289.
doi:10.2298/ABS150602016J .

Jovičić-Petrović, Jelena, Jeremić, Sanja, Vučković, Ivan M., Vojnović, Sandra, Bulajic, Aleksandra, Raicevic, Vera, Nikodinović-Runić, Jasmina, "Aspergillus Piperis A/5 from  Plum-Distilling Waste Compost Produces a Complex of Antifungal Metabolites Active Against the Phytopathogen Pythium Aphanidermatum" in Archives of biological sciences, 68, no. 2 (2016):279-289,
https://doi.org/10.2298/ABS150602016J . .