TY  - JOUR
AU  - Jurišević, Milena
AU  - Jagić, Nikola
AU  - Gajović, Nevena
AU  - Arsenijević, Aleksandar
AU  - Jovanović, Milan
AU  - Milovanović, Marija
AU  - Pantić, Jelena
AU  - Jovanović, Ivan
AU  - Sabo, Tibor
AU  - Radosavljević, Gordana
AU  - Arsenijević, Nebojša
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4238
AB  - Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.
PB  - Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer
VL  - 77
IS  - 7
SP  - 715
EP  - 723
DO  - 10.2298/VSP180723149J
ER  - 

@article{
author = "Jurišević, Milena and Jagić, Nikola and Gajović, Nevena and Arsenijević, Aleksandar and Jovanović, Milan and Milovanović, Marija and Pantić, Jelena and Jovanović, Ivan and Sabo, Tibor and Radosavljević, Gordana and Arsenijević, Nebojša",
year = "2020",
abstract = "Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.",
publisher = "Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer",
volume = "77",
number = "7",
pages = "715-723",
doi = "10.2298/VSP180723149J"
}

Jurišević, M., Jagić, N., Gajović, N., Arsenijević, A., Jovanović, M., Milovanović, M., Pantić, J., Jovanović, I., Sabo, T., Radosavljević, G.,& Arsenijević, N.. (2020). O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled
Military Medical Academy, INI., 77(7), 715-723.
https://doi.org/10.2298/VSP180723149J

Jurišević M, Jagić N, Gajović N, Arsenijević A, Jovanović M, Milovanović M, Pantić J, Jovanović I, Sabo T, Radosavljević G, Arsenijević N. O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled. 2020;77(7):715-723.
doi:10.2298/VSP180723149J .

Jurišević, Milena, Jagić, Nikola, Gajović, Nevena, Arsenijević, Aleksandar, Jovanović, Milan, Milovanović, Marija, Pantić, Jelena, Jovanović, Ivan, Sabo, Tibor, Radosavljević, Gordana, Arsenijević, Nebojša, "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer" in Vojnosanitetski pregled, 77, no. 7 (2020):715-723,
https://doi.org/10.2298/VSP180723149J . .