Nedić, Nebojša

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Authority KeyName Variants
orcid::0000-0002-7671-5139
  • Nedić, Nebojša (15)
Projects
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') Structure-properties relationships of natural and synthetic molecules and their metal complexes
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200051 (Institute of General and Physical Chemistry, Belgrade) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry)
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200358 (BioSense Institute) Utilization of plant sources of protein, dietary fiber and antioxidants in food production
Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade)
info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS/ Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200053 (University of Belgrade, Institute for Multidisciplinary Research) Studying climate change and its influence on environment: impacts, adaptation and mitigation
Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production
FUNPRO - Functional products based on goat's milk proteins and bioactive compounds extracted from grape pomace and edible mushrooms Slovenian Research Agency (research core funding No. P1- 0005).

Author's Bibliography

Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Špirović Trifunović, Bojana; Nedić, Nebojša; Gašić, Uroš M.; Tešić, Živoslav Lj.; Stanojević, Sladjana P.; Pešić, Mirjana B.

(2023)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Stanojević, Sladjana P.
AU  - Pešić, Mirjana B.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6286
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
T2  - Antioxidants
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
VL  - 12
IS  - 7
SP  - 1424
DO  - 10.3390/antiox12071424
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš M. and Tešić, Živoslav Lj. and Stanojević, Sladjana P. and Pešić, Mirjana B.",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
journal = "Antioxidants, Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
volume = "12",
number = "7",
pages = "1424",
doi = "10.3390/antiox12071424"
}
Kostić, A. Ž., Milinčić, D. D., Špirović Trifunović, B., Nedić, N., Gašić, U. M., Tešić, Ž. Lj., Stanojević, S. P.,& Pešić, M. B.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants, 12(7), 1424.
https://doi.org/10.3390/antiox12071424
Kostić AŽ, Milinčić DD, Špirović Trifunović B, Nedić N, Gašić UM, Tešić ŽL, Stanojević SP, Pešić MB. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš M., Tešić, Živoslav Lj., Stanojević, Sladjana P., Pešić, Mirjana B., "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .
4
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Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies

Stanković, Mira; Prokopijević, Miloš; Šikoparija, Branko; Nedić, Nebojša; Andrić, Filip; Polović, Natalija; Natić, Maja; Radotić, Ksenija

(2023)

TY  - JOUR
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Andrić, Filip
AU  - Polović, Natalija
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5847
AB  - Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera) colonies. This study aimed to find a promising marker in honey for the bee colony infestation level using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the infestation level was positively correlated with the phenolic spectral component (r = 0.656), which was tentatively related to the phenolics in honey. No correlation was found between the diastase activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.
T2  - Foods
T2  - Foods
T1  - Using Front-Face Fluorescence Spectroscopy and Biochemical
Analysis of Honey to Assess a Marker for the Level of Varroa
destructor Infestation of Honey Bee (Apis mellifera) Colonies
VL  - 12
IS  - 3
SP  - 629
DO  - 10.3390/foods12030629
ER  - 
@article{
author = "Stanković, Mira and Prokopijević, Miloš and Šikoparija, Branko and Nedić, Nebojša and Andrić, Filip and Polović, Natalija and Natić, Maja and Radotić, Ksenija",
year = "2023",
abstract = "Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera) colonies. This study aimed to find a promising marker in honey for the bee colony infestation level using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the infestation level was positively correlated with the phenolic spectral component (r = 0.656), which was tentatively related to the phenolics in honey. No correlation was found between the diastase activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.",
journal = "Foods, Foods",
title = "Using Front-Face Fluorescence Spectroscopy and Biochemical
Analysis of Honey to Assess a Marker for the Level of Varroa
destructor Infestation of Honey Bee (Apis mellifera) Colonies",
volume = "12",
number = "3",
pages = "629",
doi = "10.3390/foods12030629"
}
Stanković, M., Prokopijević, M., Šikoparija, B., Nedić, N., Andrić, F., Polović, N., Natić, M.,& Radotić, K.. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical
Analysis of Honey to Assess a Marker for the Level of Varroa
destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods, 12(3), 629.
https://doi.org/10.3390/foods12030629
Stanković M, Prokopijević M, Šikoparija B, Nedić N, Andrić F, Polović N, Natić M, Radotić K. Using Front-Face Fluorescence Spectroscopy and Biochemical
Analysis of Honey to Assess a Marker for the Level of Varroa
destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods. 2023;12(3):629.
doi:10.3390/foods12030629 .
Stanković, Mira, Prokopijević, Miloš, Šikoparija, Branko, Nedić, Nebojša, Andrić, Filip, Polović, Natalija, Natić, Maja, Radotić, Ksenija, "Using Front-Face Fluorescence Spectroscopy and Biochemical
Analysis of Honey to Assess a Marker for the Level of Varroa
destructor Infestation of Honey Bee (Apis mellifera) Colonies" in Foods, 12, no. 3 (2023):629,
https://doi.org/10.3390/foods12030629 . .
1
2
1
1

Elemental Profile of Propolis from Different Areas of Serbia

Ristivojević, Petar; Nešić, Jelena; Andrić, Filip; Nedić, Nebojša; Stanisavljević, Ljubiša; Milojković-Opsenica, Dušanka; Trifković, Jelena

(Wiley, 2023)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Nešić, Jelena
AU  - Andrić, Filip
AU  - Nedić, Nebojša
AU  - Stanisavljević, Ljubiša
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5895
AB  - Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.
PB  - Wiley
T2  - Chemistry & Biodiversity
T2  - Chemistry & Biodiversity
T1  - Elemental Profile of Propolis from Different Areas of Serbia
VL  - 20
IS  - 3
SP  - e202201140
DO  - 10.1002/cbdv.202201140
ER  - 
@article{
author = "Ristivojević, Petar and Nešić, Jelena and Andrić, Filip and Nedić, Nebojša and Stanisavljević, Ljubiša and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Propolis is a resinous natural substance collected by honeybees from different plant sources. The element content of propolis is influenced by the content of elements in the soil, climatic factors, and the degree of pollution. The aim of the study was to characterize element composition and content of toxic elements in 51 propolis collected from different locations in Serbia and to evaluate the differences among them. Determination of the 21 elements were performed using Inductively coupled plasma - optical emission spectrometry. K was the most abundant element, followed by Ca, Mg, Si, Fe, Zn, and Al. The microelements B, Ba, Mn, Na, Pb, and Ti were also present in propolis, while Co, Cr, Cu, Mo, Ni, Sr, and V were found in trace. Toxic elements such as Pb, As, and Cd were found in the trace. Kruskal-Wallis tests differentiate groups of samples by geographical origin. The presence of B, Fe, Sr, Ti, Zn, and As demonstrated statistical significance between six regions of Serbia.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity, Chemistry & Biodiversity",
title = "Elemental Profile of Propolis from Different Areas of Serbia",
volume = "20",
number = "3",
pages = "e202201140",
doi = "10.1002/cbdv.202201140"
}
Ristivojević, P., Nešić, J., Andrić, F., Nedić, N., Stanisavljević, L., Milojković-Opsenica, D.,& Trifković, J.. (2023). Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity
Wiley., 20(3), e202201140.
https://doi.org/10.1002/cbdv.202201140
Ristivojević P, Nešić J, Andrić F, Nedić N, Stanisavljević L, Milojković-Opsenica D, Trifković J. Elemental Profile of Propolis from Different Areas of Serbia. in Chemistry & Biodiversity. 2023;20(3):e202201140.
doi:10.1002/cbdv.202201140 .
Ristivojević, Petar, Nešić, Jelena, Andrić, Filip, Nedić, Nebojša, Stanisavljević, Ljubiša, Milojković-Opsenica, Dušanka, Trifković, Jelena, "Elemental Profile of Propolis from Different Areas of Serbia" in Chemistry & Biodiversity, 20, no. 3 (2023):e202201140,
https://doi.org/10.1002/cbdv.202201140 . .
1
1
1
1

Honey with added value – enriched with rutin and quercetin from Sophora flower

Đorđević, Sofija; Nedić, Nebojša; Pavlović, Aleksandra; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.; Gašić, Uroš M.

(Elsevier, 2022)

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
AU  - Gašić, Uroš M.
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S2210803322000495
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5410
AB  - IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
PB  - Elsevier
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
SP  - 100580
DO  - 10.1016/j.hermed.2022.100580
ER  - 
@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Gašić, Uroš M.",
year = "2022",
abstract = "IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
publisher = "Elsevier",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
pages = "100580",
doi = "10.1016/j.hermed.2022.100580"
}
Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Gašić, U. M.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine
Elsevier., 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580
Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić ŽL, Gašić UM. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .
Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., Gašić, Uroš M., "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .
3
2
2

Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia

Nedić, Nebojša; Nešović, Milica; Radišić, Predrag; Gašić, Uroš M.; Baošić, Rada; Joksimović, Kristina; Pezo, Lato; Tešić, Živoslav Lj.; Vovk, Irena

(Frontiers Media S.A., 2022)

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš M.
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav Lj.
AU  - Vovk, Irena
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5505
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia
VL  - 9
SP  - 941463
DO  - 10.3389/fnut.2022.941463
ER  - 
@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš M. and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav Lj. and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition, Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia",
volume = "9",
pages = "941463",
doi = "10.3389/fnut.2022.941463"
}
Nedić, N., Nešović, M., Radišić, P., Gašić, U. M., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž. Lj.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition
Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463
Nedić N, Nešović M, Radišić P, Gašić UM, Baošić R, Joksimović K, Pezo L, Tešić ŽL, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .
Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš M., Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav Lj., Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia" in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .
1
18
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5

Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Nedić, Nebojša; Gašić, Uroš M.; Špirović Trifunović, Bojana; Vojt, Denis; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(MDPI, 2021)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4622
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI
T2  - Antioxidants
T1  - Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen
VL  - 10
IS  - 7
SP  - 1091
DO  - 10.3390/antiox10071091
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen",
volume = "10",
number = "7",
pages = "1091",
doi = "10.3390/antiox10071091"
}
Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants
MDPI., 10(7), 1091.
https://doi.org/10.3390/antiox10071091
Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .
22
2
17
15

Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Nedić, Nebojša; Gašić, Uroš M.; Špirović Trifunović, Bojana; Vojt, Denis; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(MDPI, 2021)

TY  - DATA
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4623
PB  - MDPI
T2  - Antioxidants
T1  - Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4623
ER  - 
@misc{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
publisher = "MDPI",
journal = "Antioxidants",
title = "Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4623"
}
Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.. in Antioxidants
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_4623
Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.. in Antioxidants. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4623 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091." in Antioxidants (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4623 .

Distribution of polyphenolic and sugar compounds in different buckwheat plant parts

Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Horvacki, Nikola; Nedić, Nebojša; Sredojević, Milica; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.

(Royal Society of Chemistry, 2021)

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav Lj.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4739
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
VL  - 11
IS  - 42
SP  - 25816
EP  - 25829
DO  - 10.1039/D1RA04250E
ER  - 
@article{
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
volume = "11",
number = "42",
pages = "25816-25829",
doi = "10.1039/D1RA04250E"
}
Nešović, M., Gašić, U. M., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/D1RA04250E
Nešović M, Gašić UM, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić ŽL. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/D1RA04250E .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/D1RA04250E . .
1
26
1
22
19

Polyphenol profile of buckwheat honey, nectar and pollen

Nešović, Milica; Gašić, Uroš M.; Tosti, Tomislav; Horvacki, Nikola; Šikoparija, Branko; Nedić, Nebojša; Blagojević, Stevan; Ignjatović, Ljubiša; Tešić, Živoslav Lj.

(The Royal Society, 2020)

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav Lj.
PY  - 2020
UR  - https://royalsocietypublishing.org/doi/10.1098/rsos.201576
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4451
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - The Royal Society
T2  - Royal Society Open Science
T1  - Polyphenol profile of buckwheat honey, nectar and pollen
VL  - 7
IS  - 12
SP  - 201576
DO  - 10.1098/rsos.201576
ER  - 
@article{
author = "Nešović, Milica and Gašić, Uroš M. and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav Lj.",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "The Royal Society",
journal = "Royal Society Open Science",
title = "Polyphenol profile of buckwheat honey, nectar and pollen",
volume = "7",
number = "12",
pages = "201576",
doi = "10.1098/rsos.201576"
}
Nešović, M., Gašić, U. M., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž. Lj.. (2020). Polyphenol profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
The Royal Society., 7(12), 201576.
https://doi.org/10.1098/rsos.201576
Nešović M, Gašić UM, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić ŽL. Polyphenol profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .
Nešović, Milica, Gašić, Uroš M., Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav Lj., "Polyphenol profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .
22
2
16

Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia

Kostić, Aleksandar Ž.; Dojčinović, Biljana P.; Nedić, Nebojša; Milinčić, Danijel D.; Pantelić, Nebojša Ð.; Stanojević, Slađana P.; Barać, Miroljub B.; Tešić, Živoslav Lj.; Pešić, Mirjana B.; Brka, Muhamed; Omanović-Mikličanin, Enisa; Karić, Lutvija; Falan, Vedad; Toroman, Almir

(Springer International Publishing, 2020)

TY  - CONF
AU  - Kostić, Aleksandar Ž.
AU  - Dojčinović, Biljana P.
AU  - Nedić, Nebojša
AU  - Milinčić, Danijel D.
AU  - Pantelić, Nebojša Ð.
AU  - Stanojević, Slađana P.
AU  - Barać, Miroljub B.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
AU  - Brka, Muhamed
AU  - Omanović-Mikličanin, Enisa
AU  - Karić, Lutvija
AU  - Falan, Vedad
AU  - Toroman, Almir
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4279
AB  - Pollen (floral or bee-collected) can be used as a good source of different nutrients including several important biogenic elements (minerals). The aim of this study was to determine content of selected biogenic elements (phosphorous, potassium, sulphur, calcium, magnesium, iron and zinc) in monofloral bee-collected pollen originated from sunflower (Helianthus annuus L.) plants grown in Serbia and to estimate its nutritional value regarding to elements content. In that purpose ICP-OES analytical method was applied. According to obtained results the examined pollen sample contains above mentioned elements in following quantities: P – 3523.98 mg/kg; K – 2869.13 mg/kg; S – 1951.74 mg/kg; Ca – 1919.20 mg/kg; Mg – 654.36 mg/kg; Fe –83.55 mg/kg; Zn – 45.92 mg/kg of dry weight. Based on recommended daily intakes for iron, phosphorus and zinc application of pollen, as food or food ingredient, will cover meaningful part of human needs for these three important elements.
PB  - Springer International Publishing
C3  - 30th Scientific-Experts Conference of Agriculture and Food Industry
T1  - Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia
SP  - 211
EP  - 217
DO  - 10.1007/978-3-030-40049-1_27
ER  - 
@conference{
author = "Kostić, Aleksandar Ž. and Dojčinović, Biljana P. and Nedić, Nebojša and Milinčić, Danijel D. and Pantelić, Nebojša Ð. and Stanojević, Slađana P. and Barać, Miroljub B. and Tešić, Živoslav Lj. and Pešić, Mirjana B. and Brka, Muhamed and Omanović-Mikličanin, Enisa and Karić, Lutvija and Falan, Vedad and Toroman, Almir",
year = "2020",
abstract = "Pollen (floral or bee-collected) can be used as a good source of different nutrients including several important biogenic elements (minerals). The aim of this study was to determine content of selected biogenic elements (phosphorous, potassium, sulphur, calcium, magnesium, iron and zinc) in monofloral bee-collected pollen originated from sunflower (Helianthus annuus L.) plants grown in Serbia and to estimate its nutritional value regarding to elements content. In that purpose ICP-OES analytical method was applied. According to obtained results the examined pollen sample contains above mentioned elements in following quantities: P – 3523.98 mg/kg; K – 2869.13 mg/kg; S – 1951.74 mg/kg; Ca – 1919.20 mg/kg; Mg – 654.36 mg/kg; Fe –83.55 mg/kg; Zn – 45.92 mg/kg of dry weight. Based on recommended daily intakes for iron, phosphorus and zinc application of pollen, as food or food ingredient, will cover meaningful part of human needs for these three important elements.",
publisher = "Springer International Publishing",
journal = "30th Scientific-Experts Conference of Agriculture and Food Industry",
title = "Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia",
pages = "211-217",
doi = "10.1007/978-3-030-40049-1_27"
}
Kostić, A. Ž., Dojčinović, B. P., Nedić, N., Milinčić, D. D., Pantelić, N. Ð., Stanojević, S. P., Barać, M. B., Tešić, Ž. Lj., Pešić, M. B., Brka, M., Omanović-Mikličanin, E., Karić, L., Falan, V.,& Toroman, A.. (2020). Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia. in 30th Scientific-Experts Conference of Agriculture and Food Industry
Springer International Publishing., 211-217.
https://doi.org/10.1007/978-3-030-40049-1_27
Kostić AŽ, Dojčinović BP, Nedić N, Milinčić DD, Pantelić NÐ, Stanojević SP, Barać MB, Tešić ŽL, Pešić MB, Brka M, Omanović-Mikličanin E, Karić L, Falan V, Toroman A. Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia. in 30th Scientific-Experts Conference of Agriculture and Food Industry. 2020;:211-217.
doi:10.1007/978-3-030-40049-1_27 .
Kostić, Aleksandar Ž., Dojčinović, Biljana P., Nedić, Nebojša, Milinčić, Danijel D., Pantelić, Nebojša Ð., Stanojević, Slađana P., Barać, Miroljub B., Tešić, Živoslav Lj., Pešić, Mirjana B., Brka, Muhamed, Omanović-Mikličanin, Enisa, Karić, Lutvija, Falan, Vedad, Toroman, Almir, "Content and Nutritional Value of Selected Biogenic Elements in Monofloral Sunflower Bee-Collected Pollen from Serbia" in 30th Scientific-Experts Conference of Agriculture and Food Industry (2020):211-217,
https://doi.org/10.1007/978-3-030-40049-1_27 . .
2
2
1

Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Gašić, Uroš M.; Nedić, Nebojša; Stanojević, Slađana P.; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(Academic Press, 2019)

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Gašić, Uroš M.
AU  - Nedić, Nebojša
AU  - Stanojević, Slađana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3713
AB  - The aim of this work was to determine phenolic profile and antioxidant properties of methanolic (MEP) and ethanolic (EEP) extracts of monofloral bee-collected sunflower pollen from Serbia. In total, 37 different polyphenols (19 flavonol glycosides and 18 other polyphenols) were identified by UHPLC/MS-MS Orbitrap analysis. The different phenolic profile of MEP and EEP was observed with flavonols as most abundant, 94.1% (MEP) and 93.4% (EEP), out of which flavonoid glycosides represented 73.2% (MEP) and 66.1% (EEP). The higher amount of total phenolics was recorded in EEP (200.58 mg/kg DW) than in MEP (244.44 mg/kg DW). The quercetin 3-O-galactoside was the major compound in both extracts, 112.86 mg/kg in MEP and 128.64 mg/kg in EEP. The presence of coumaroylated and acetylated flavonoid glycosides were registered, among which the quercetin 3-O-(6“-acetyl)hexoside was recored in the significant amount in MEP (111.10 mg Q3G/kg DW), whereas in EEP the content of coumaroylated kaempferol glycosides (137.95 mg Q3G/kg DW) was significantly higher. The ferric reducing power and ABTS radical scavenging activity assays revealed that pollen extracts possessed significant antioxidant activity. The monofloral bee-collected pollen of Helianthus annuus L. can be used as good food supplement with high flavonoids content and antioxidant activity.
PB  - Academic Press
T2  - LWT
T1  - Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant
VL  - 112
DO  - 10.1016/j.lwt.2019.06.011
ER  - 
@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Gašić, Uroš M. and Nedić, Nebojša and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2019",
abstract = "The aim of this work was to determine phenolic profile and antioxidant properties of methanolic (MEP) and ethanolic (EEP) extracts of monofloral bee-collected sunflower pollen from Serbia. In total, 37 different polyphenols (19 flavonol glycosides and 18 other polyphenols) were identified by UHPLC/MS-MS Orbitrap analysis. The different phenolic profile of MEP and EEP was observed with flavonols as most abundant, 94.1% (MEP) and 93.4% (EEP), out of which flavonoid glycosides represented 73.2% (MEP) and 66.1% (EEP). The higher amount of total phenolics was recorded in EEP (200.58 mg/kg DW) than in MEP (244.44 mg/kg DW). The quercetin 3-O-galactoside was the major compound in both extracts, 112.86 mg/kg in MEP and 128.64 mg/kg in EEP. The presence of coumaroylated and acetylated flavonoid glycosides were registered, among which the quercetin 3-O-(6“-acetyl)hexoside was recored in the significant amount in MEP (111.10 mg Q3G/kg DW), whereas in EEP the content of coumaroylated kaempferol glycosides (137.95 mg Q3G/kg DW) was significantly higher. The ferric reducing power and ABTS radical scavenging activity assays revealed that pollen extracts possessed significant antioxidant activity. The monofloral bee-collected pollen of Helianthus annuus L. can be used as good food supplement with high flavonoids content and antioxidant activity.",
publisher = "Academic Press",
journal = "LWT",
title = "Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant",
volume = "112",
doi = "10.1016/j.lwt.2019.06.011"
}
Kostić, A. Ž., Milinčić, D. D., Gašić, U. M., Nedić, N., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2019). Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant. in LWT
Academic Press., 112.
https://doi.org/10.1016/j.lwt.2019.06.011
Kostić AŽ, Milinčić DD, Gašić UM, Nedić N, Stanojević SP, Tešić ŽL, Pešić MB. Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant. in LWT. 2019;112.
doi:10.1016/j.lwt.2019.06.011 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Gašić, Uroš M., Nedić, Nebojša, Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant" in LWT, 112 (2019),
https://doi.org/10.1016/j.lwt.2019.06.011 . .
77
31
74
59

Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011

Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Gašić, Uroš M.; Nedić, Nebojša; Stanojević, Slađana P.; Tešić, Živoslav Lj.; Pešić, Mirjana B.

(Academic Press, 2019)

TY  - DATA
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Gašić, Uroš M.
AU  - Nedić, Nebojša
AU  - Stanojević, Slađana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3714
PB  - Academic Press
T2  - LWT
T1  - Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3714
ER  - 
@misc{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Gašić, Uroš M. and Nedić, Nebojša and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2019",
publisher = "Academic Press",
journal = "LWT",
title = "Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3714"
}
Kostić, A. Ž., Milinčić, D. D., Gašić, U. M., Nedić, N., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2019). Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011. in LWT
Academic Press..
https://hdl.handle.net/21.15107/rcub_cherry_3714
Kostić AŽ, Milinčić DD, Gašić UM, Nedić N, Stanojević SP, Tešić ŽL, Pešić MB. Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011. in LWT. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3714 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Gašić, Uroš M., Nedić, Nebojša, Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Gašić, U. M.; Nedić, N.; Stanojević, S. P.; Tešić, Ž. L.; Pešić, M. B. Polyphenolic Profile and Antioxidant Properties of Bee-Collected Pollen from Sunflower (Helianthus Annuus L.) Plant. LWT 2019, 112. https://doi.org/10.1016/j.lwt.2019.06.011" in LWT (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3714 .

Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar

Fotirić-Akšić, Milica M.; Tosti, Tomislav; Nedić, Nebojša; Markovic, Misa; Ličina, Vlado; Milojković-Opsenica, Dušanka; Tešić, Živoslav Lj.

(Springer Heidelberg, Heidelberg, 2015)

TY  - JOUR
AU  - Fotirić-Akšić, Milica M.
AU  - Tosti, Tomislav
AU  - Nedić, Nebojša
AU  - Markovic, Misa
AU  - Ličina, Vlado
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1881
AB  - Cold stress adversely affects growth and productivity, and triggers a series of morphological, physiological, biochemical and molecular changes in plants. Since sugars are present in all floral nectars in greater amounts than any other constituent, the aim of this study was to examine how frost exposure changes sugar metabolism and how it affects on the content of sugar components in the nectar of quince. Three quince cultivars ('Vranjska', 'Triumph' and 'Leskovac. ka') were investigated in this study. The contents of sugars (glucose, fructose, sucrose, trehalose, maltose, isomaltose, rhamnose, arabinose, ribose, melezitose, raffinose, and panose) and sugar alcohols (sorbitol, erythritol, mannitol and galactitol) were analyzed by high performance anion exchange chromatography (HPAEC) with amperometric detection. The results showed that after late spring frosts and irreversible damage of flower parts, the nectar of the three quince cultivars contained elevated levels of fructose, trehalose, arabinose, ribose, rhamnose, raffinose, galactitol and mannitol, indicating an impairment of central carbohydrate metabolism. The ratios between individual sugars, such as the glucose/fructose ratio, were changed in the nectar of damaged flowers in all three quince cultivars. The examined cultivars showed similar sugar response to cold stress. The only exception was 'Leskovac. ka' for the glucose and melezitose pathway, which means that composition of those two sugars changed significantly according to the genotype. The larger are the carbohydrates reserves in different parts of a fruit tree, the higher is the tolerance to any form of frost damage, the results of this study could help in the understanding of how different quince cultivars react to this kind of stress and how they modulate their sugar metabolism.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar
VL  - 37
IS  - 1
DO  - 10.1007/s11738-014-1701-y
ER  - 
@article{
author = "Fotirić-Akšić, Milica M. and Tosti, Tomislav and Nedić, Nebojša and Markovic, Misa and Ličina, Vlado and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj.",
year = "2015",
abstract = "Cold stress adversely affects growth and productivity, and triggers a series of morphological, physiological, biochemical and molecular changes in plants. Since sugars are present in all floral nectars in greater amounts than any other constituent, the aim of this study was to examine how frost exposure changes sugar metabolism and how it affects on the content of sugar components in the nectar of quince. Three quince cultivars ('Vranjska', 'Triumph' and 'Leskovac. ka') were investigated in this study. The contents of sugars (glucose, fructose, sucrose, trehalose, maltose, isomaltose, rhamnose, arabinose, ribose, melezitose, raffinose, and panose) and sugar alcohols (sorbitol, erythritol, mannitol and galactitol) were analyzed by high performance anion exchange chromatography (HPAEC) with amperometric detection. The results showed that after late spring frosts and irreversible damage of flower parts, the nectar of the three quince cultivars contained elevated levels of fructose, trehalose, arabinose, ribose, rhamnose, raffinose, galactitol and mannitol, indicating an impairment of central carbohydrate metabolism. The ratios between individual sugars, such as the glucose/fructose ratio, were changed in the nectar of damaged flowers in all three quince cultivars. The examined cultivars showed similar sugar response to cold stress. The only exception was 'Leskovac. ka' for the glucose and melezitose pathway, which means that composition of those two sugars changed significantly according to the genotype. The larger are the carbohydrates reserves in different parts of a fruit tree, the higher is the tolerance to any form of frost damage, the results of this study could help in the understanding of how different quince cultivars react to this kind of stress and how they modulate their sugar metabolism.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar",
volume = "37",
number = "1",
doi = "10.1007/s11738-014-1701-y"
}
Fotirić-Akšić, M. M., Tosti, T., Nedić, N., Markovic, M., Ličina, V., Milojković-Opsenica, D.,& Tešić, Ž. Lj.. (2015). Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 37(1).
https://doi.org/10.1007/s11738-014-1701-y
Fotirić-Akšić MM, Tosti T, Nedić N, Markovic M, Ličina V, Milojković-Opsenica D, Tešić ŽL. Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar. in Acta Physiologiae Plantarum. 2015;37(1).
doi:10.1007/s11738-014-1701-y .
Fotirić-Akšić, Milica M., Tosti, Tomislav, Nedić, Nebojša, Markovic, Misa, Ličina, Vlado, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., "Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar" in Acta Physiologiae Plantarum, 37, no. 1 (2015),
https://doi.org/10.1007/s11738-014-1701-y . .
34
15
30
27

Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis

Ristivojević, Petar; Trifković, Jelena; Gašić, Uroš M.; Andrić, Filip; Nedić, Nebojša; Tešić, Živoslav Lj.; Milojković-Opsenica, Dušanka

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Trifković, Jelena
AU  - Gašić, Uroš M.
AU  - Andrić, Filip
AU  - Nedić, Nebojša
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1480
AB  - IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahigh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin. Copyright (c) 2014 John Wiley & Sons, Ltd. The phenolic profiles of Serbian propolis samples were determined using ultrahigh-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser. Fingerprint analysis revealed the existence of 75 phenolic compounds. Eight of them were identified in propolis for the first time. The chemical composition of the samples analysed confirmed their affiliation to the European poplar propolis and the existence of two subgroups according to their phenolic profile.
PB  - Wiley-Blackwell, Hoboken
T2  - Phytochemical Analysis
T1  - Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis
VL  - 26
IS  - 2
SP  - 127
EP  - 136
DO  - 10.1002/pca.2544
ER  - 
@article{
author = "Ristivojević, Petar and Trifković, Jelena and Gašić, Uroš M. and Andrić, Filip and Nedić, Nebojša and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2015",
abstract = "IntroductionPropolis is a resinous natural substance collected by honeybees from different plant sources. Due to the presence of various phytochemicals, this bee-product exhibits numerous biological activities, including anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, immunostimulating and anti-tumour effects. As the chemical composition and biological activity of propolis depend on its botanical and geographical origin, searching for new bioactive substances in various types of propolis from unexplored regions is of great importance. ObjectiveThe aim of this study is the evaluation of the phenolic profile of poplar propolis samples in order to characterise Serbian propolis, to identify possible new constituents and to specify the phenolic components relevant for differentiation of poplar propolis samples into two subgroups through simultaneous analysis of poplar bud extracts. MethodsEthanolic extracts of propolis and poplar buds were comprehensively analysed using ultrahigh-performance liquid chromatography coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser together with chemometric methods. ResultsExtensive fingerprint analysis of Serbian propolis was achieved for the first time. Seventy-five phenolic compounds were detected. Eight of them were identified in propolis for the first time. Pattern-recognition methods applied to the content of ten quantified phenolics verified the existence of two subgroups of propolis, with galangin, chrysin and pinocembrin as the most influential distinguishing factors. ConclusionThe phenolic composition of the analysed propolis samples confirm their affiliation to the European poplar type propolis and the existence of two subgroups according to botanical origin. Copyright (c) 2014 John Wiley & Sons, Ltd. The phenolic profiles of Serbian propolis samples were determined using ultrahigh-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry, which combines the linear trap quadrupole and Orbitrap MS/MS mass analyser. Fingerprint analysis revealed the existence of 75 phenolic compounds. Eight of them were identified in propolis for the first time. The chemical composition of the samples analysed confirmed their affiliation to the European poplar propolis and the existence of two subgroups according to their phenolic profile.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytochemical Analysis",
title = "Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis",
volume = "26",
number = "2",
pages = "127-136",
doi = "10.1002/pca.2544"
}
Ristivojević, P., Trifković, J., Gašić, U. M., Andrić, F., Nedić, N., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2015). Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. in Phytochemical Analysis
Wiley-Blackwell, Hoboken., 26(2), 127-136.
https://doi.org/10.1002/pca.2544
Ristivojević P, Trifković J, Gašić UM, Andrić F, Nedić N, Tešić ŽL, Milojković-Opsenica D. Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. in Phytochemical Analysis. 2015;26(2):127-136.
doi:10.1002/pca.2544 .
Ristivojević, Petar, Trifković, Jelena, Gašić, Uroš M., Andrić, Filip, Nedić, Nebojša, Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Ultrahigh-performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis" in Phytochemical Analysis, 26, no. 2 (2015):127-136,
https://doi.org/10.1002/pca.2544 . .
72
48
69
64

Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544

Ristivojević, Petar; Trifković, Jelena; Gašić, Uroš M.; Andrić, Filip; Nedić, Nebojša; Tešić, Živoslav Lj.; Milojković-Opsenica, Dušanka

(Wiley-Blackwell, Hoboken, 2015)

TY  - DATA
AU  - Ristivojević, Petar
AU  - Trifković, Jelena
AU  - Gašić, Uroš M.
AU  - Andrić, Filip
AU  - Nedić, Nebojša
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3458
PB  - Wiley-Blackwell, Hoboken
T2  - Phytochemical Analysis
T1  - Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3458
ER  - 
@misc{
author = "Ristivojević, Petar and Trifković, Jelena and Gašić, Uroš M. and Andrić, Filip and Nedić, Nebojša and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytochemical Analysis",
title = "Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3458"
}
Ristivojević, P., Trifković, J., Gašić, U. M., Andrić, F., Nedić, N., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2015). Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544. in Phytochemical Analysis
Wiley-Blackwell, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3458
Ristivojević P, Trifković J, Gašić UM, Andrić F, Nedić N, Tešić ŽL, Milojković-Opsenica D. Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544. in Phytochemical Analysis. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3458 .
Ristivojević, Petar, Trifković, Jelena, Gašić, Uroš M., Andrić, Filip, Nedić, Nebojša, Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Supplementary data for article: Ristivojević, P.; Trifković, J.; Gašić, U. M.; Andrić, F.; Nedic, N.; Tešić, Ž. L.; Milojković-Opsenica, D. Ultrahigh-Performance Liquid Chromatography and Mass Spectrometry (UHPLC-LTQ/Orbitrap/MS/MS) Study of Phenolic Profile of Serbian Poplar Type Propolis. Phytochemical Analysis 2015, 26 (2), 127–136. https://doi.org/10.1002/pca.2544" in Phytochemical Analysis (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3458 .