TY  - THES
AU  - Lazarević-Pašti, Tamara
PY  - 2012
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=332
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:5873/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=41104655
UR  - http://nardus.mpn.gov.rs/123456789/3468
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2612
AB  - Ispitana je inhibicija slobodne acetilholinesteraze (AChE) odabranim organotiofosfatima (OP) (diazinon, malation, hlorpirifos, azinfos-metil, forat) i njihovim oksoanalozima (diazokson, malaokson, hlorpirifos-okson, azinfos-metil-okson, forat-okson), pri čemu su optimizovani uslovi za detekciju najniže koncentracije tih jedinjenja primenom AChE testa. Određene su IC50 vrednosti za sva navedena jedinjenja. U cilju povećanja osetljivosti AChE testa, ispitivani organo-tiofosfati prevedeni su u okso-analoge u prisustvu enzima mijeloperoksidaze (MPO). To je potvreno pomoćuUPLC i GC/MS analize. Nastali oksidacioni proizvodi stabilni su najmanje 1h. Maksimalne koncentracije oksona dobijaju se kada se organo-tiofosfati inkubiraju sa 100 nM MPO u prisustvu H2O2 koncentracije 50 μM, pri pH 6, na temperaturi 25 °C u toku 10 minuta. Oksidacija OP u prisustvu MPO pod navedenim uslovima primenjena je u modifikaciji metode za detekciju OP na bazi inhibicije AChE. Određena je granica detekcije modifikovane metode, kao i efikasnost oksidacije i njena primena na smešu OP. Gore navedeni organo-tiofosfati prevedeni su u odgovarajue oksone i pomoću elektrohemijski generisanih halogena. Proizvodi koji nastaju identifikovani su kao oksoni pomoću UPLC analize. Utvrđeno je da je najefikasnija oksidacija elektrogenerisanim bromom u toku 15 minuta. Određena je i granica detekcije AChE testa za odreivanje OP kada su OP oksidovani elektrohemijski generisanim bromom...
AB  - The inhibition of acetylcholinesterase in the presence of selected organothiophosphates (diazinon, malathion, chlorpyrifos, azinphos-methyl, phorate) andtheir oxo-analogues (diazoxon, malaoxon, chlorpyrifos-oxon, azinphos-methyl-oxon,phorate-oxon) was examined. Experimental conditions were optimized to detect the lowestposible concentrations of these compounds using AChE test. IC50 values were determinedfor all the mentioned compounds.Investigated organothiophosphates have been converted into their oxo-analogues inthe presence of the enzyme myeloperoxidase. Oxidation products were detected usingUPLC and GC/MS analysis. It was found that the products are stable within minimum 1h.In order to optimize oxidation procedure and achieve maximum concentrations of oxons,the optimal concentrations of H2O2 (50 μM) and MPO (100 nM) were determined. Also,the optimal incubation time of OPs and MPO (10 min), as well as the optimal pH (6) andtemperature (25 °C) were estimated. Oxidation of OPs in the presence of MPO underoptimal conditions was applied as a modification of the method for detecting OP usingAChE test. The detection limits were determined for the modified method, as well as theefficiency of oxidation and its application for the analysi of sintetic mixture of OPs.Examined organothiophosphates have been converted into their oxo-forms usingelectrochemically generated halogens. Products have been identified as oxons using UPLCanalysis. It was found that the the most efficient oxidation of OPs was carried out by after15 minutes of oxidation with electrogenerated bromine. Detection limits were determined,too.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze
T1  - Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3468
ER  - 

@phdthesis{
author = "Lazarević-Pašti, Tamara",
year = "2012",
abstract = "Ispitana je inhibicija slobodne acetilholinesteraze (AChE) odabranim organotiofosfatima (OP) (diazinon, malation, hlorpirifos, azinfos-metil, forat) i njihovim oksoanalozima (diazokson, malaokson, hlorpirifos-okson, azinfos-metil-okson, forat-okson), pri čemu su optimizovani uslovi za detekciju najniže koncentracije tih jedinjenja primenom AChE testa. Određene su IC50 vrednosti za sva navedena jedinjenja. U cilju povećanja osetljivosti AChE testa, ispitivani organo-tiofosfati prevedeni su u okso-analoge u prisustvu enzima mijeloperoksidaze (MPO). To je potvreno pomoćuUPLC i GC/MS analize. Nastali oksidacioni proizvodi stabilni su najmanje 1h. Maksimalne koncentracije oksona dobijaju se kada se organo-tiofosfati inkubiraju sa 100 nM MPO u prisustvu H2O2 koncentracije 50 μM, pri pH 6, na temperaturi 25 °C u toku 10 minuta. Oksidacija OP u prisustvu MPO pod navedenim uslovima primenjena je u modifikaciji metode za detekciju OP na bazi inhibicije AChE. Određena je granica detekcije modifikovane metode, kao i efikasnost oksidacije i njena primena na smešu OP. Gore navedeni organo-tiofosfati prevedeni su u odgovarajue oksone i pomoću elektrohemijski generisanih halogena. Proizvodi koji nastaju identifikovani su kao oksoni pomoću UPLC analize. Utvrđeno je da je najefikasnija oksidacija elektrogenerisanim bromom u toku 15 minuta. Određena je i granica detekcije AChE testa za odreivanje OP kada su OP oksidovani elektrohemijski generisanim bromom..., The inhibition of acetylcholinesterase in the presence of selected organothiophosphates (diazinon, malathion, chlorpyrifos, azinphos-methyl, phorate) andtheir oxo-analogues (diazoxon, malaoxon, chlorpyrifos-oxon, azinphos-methyl-oxon,phorate-oxon) was examined. Experimental conditions were optimized to detect the lowestposible concentrations of these compounds using AChE test. IC50 values were determinedfor all the mentioned compounds.Investigated organothiophosphates have been converted into their oxo-analogues inthe presence of the enzyme myeloperoxidase. Oxidation products were detected usingUPLC and GC/MS analysis. It was found that the products are stable within minimum 1h.In order to optimize oxidation procedure and achieve maximum concentrations of oxons,the optimal concentrations of H2O2 (50 μM) and MPO (100 nM) were determined. Also,the optimal incubation time of OPs and MPO (10 min), as well as the optimal pH (6) andtemperature (25 °C) were estimated. Oxidation of OPs in the presence of MPO underoptimal conditions was applied as a modification of the method for detecting OP usingAChE test. The detection limits were determined for the modified method, as well as theefficiency of oxidation and its application for the analysi of sintetic mixture of OPs.Examined organothiophosphates have been converted into their oxo-forms usingelectrochemically generated halogens. Products have been identified as oxons using UPLCanalysis. It was found that the the most efficient oxidation of OPs was carried out by after15 minutes of oxidation with electrogenerated bromine. Detection limits were determined,too.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze, Application of organothiophosphate pesticides oxidation in methods for their detection based on acetylcholinesterase inhibition",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3468"
}

Lazarević-Pašti, T.. (2012). Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_3468

Lazarević-Pašti T. Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze. in Универзитет у Београду. 2012;.
https://hdl.handle.net/21.15107/rcub_nardus_3468 .

Lazarević-Pašti, Tamara, "Primena oksidacije organo-tiofosfatnih pesticida u metodama za njihovu detekciju na bazi inhibicije acetilholinesteraze" in Универзитет у Београду (2012),
https://hdl.handle.net/21.15107/rcub_nardus_3468 .

TY  - JOUR
AU  - Pasti, Tamara Lazarevic
AU  - Momic, Tatjana
AU  - Onjia, Antonije E.
AU  - Vujisić, Ljubodrag V.
AU  - Vasic, Vesna
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1106
AB  - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
PB  - Springer Wien, Wien
T2  - Microchimica Acta
T1  - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
VL  - 170
IS  - 3-4
SP  - 289
EP  - 297
DO  - 10.1007/s00604-010-0324-2
ER  - 

@article{
author = "Pasti, Tamara Lazarevic and Momic, Tatjana and Onjia, Antonije E. and Vujisić, Ljubodrag V. and Vasic, Vesna",
year = "2010",
abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.",
publisher = "Springer Wien, Wien",
journal = "Microchimica Acta",
title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods",
volume = "170",
number = "3-4",
pages = "289-297",
doi = "10.1007/s00604-010-0324-2"
}

Pasti, T. L., Momic, T., Onjia, A. E., Vujisić, L. V.,& Vasic, V.. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta
Springer Wien, Wien., 170(3-4), 289-297.
https://doi.org/10.1007/s00604-010-0324-2

Pasti TL, Momic T, Onjia AE, Vujisić LV, Vasic V. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta. 2010;170(3-4):289-297.
doi:10.1007/s00604-010-0324-2 .

Pasti, Tamara Lazarevic, Momic, Tatjana, Onjia, Antonije E., Vujisić, Ljubodrag V., Vasic, Vesna, "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" in Microchimica Acta, 170, no. 3-4 (2010):289-297,
https://doi.org/10.1007/s00604-010-0324-2 . .