TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Đokić, Ivana
AU  - Tosti, Tomislav
AU  - Popović, Slađana
AU  - Milojković-Opsenica, Dušanka
AU  - Vukojević, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6385
AB  - The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.
PB  - Cellulose Chemistry and Technology
T2  - Cellulose Chemistry and Technology
T1  - WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS
VL  - 57
IS  - 7-8
SP  - 815
EP  - 828
DO  - 10.35812/CelluloseChemTechnol.2023.57.72
ER  - 

@article{
author = "Knežević, Aleksandar and Đokić, Ivana and Tosti, Tomislav and Popović, Slađana and Milojković-Opsenica, Dušanka and Vukojević, Jelena",
year = "2023",
abstract = "The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main challenges in fungal pretreatment of lignocellulosic feedstock.",
publisher = "Cellulose Chemistry and Technology",
journal = "Cellulose Chemistry and Technology",
title = "WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS",
volume = "57",
number = "7-8",
pages = "815-828",
doi = "10.35812/CelluloseChemTechnol.2023.57.72"
}

Knežević, A., Đokić, I., Tosti, T., Popović, S., Milojković-Opsenica, D.,& Vukojević, J.. (2023). WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS. in Cellulose Chemistry and Technology
Cellulose Chemistry and Technology., 57(7-8), 815-828.
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72

Knežević A, Đokić I, Tosti T, Popović S, Milojković-Opsenica D, Vukojević J. WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS. in Cellulose Chemistry and Technology. 2023;57(7-8):815-828.
doi:10.35812/CelluloseChemTechnol.2023.57.72 .

Knežević, Aleksandar, Đokić, Ivana, Tosti, Tomislav, Popović, Slađana, Milojković-Opsenica, Dušanka, Vukojević, Jelena, "WHITE-ROT FUNGAL PRETREATMENT OF WHEAT STRAW: EFFECT ON ENZYMATIC HYDROLYSIS OF CARBOHYDRATE POLYMERS" in Cellulose Chemistry and Technology, 57, no. 7-8 (2023):815-828,
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72 . .

TY  - JOUR
AU  - Sofrenić, Ivana V.
AU  - Anđelković, Boban D.
AU  - Vujisić, Ljubodrag V.
AU  - Novaković, Miroslav M.
AU  - Knežević, Aleksandar
AU  - Stanković, Miroslava

AU  - Milosavljević, Slobodan M.
AU  - Tešević, Vele
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4819
AB  - Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds showed a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in the concentration dependent manner, with the concentration of 2.0 µg mL-1 being the most effective – with increase of the concentration the activity slightly decreases. The structure–activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than Δ8-analogues. It was also demonstrated that the DNA protective activities within the Δ8-lanostane-26-oic acid group are affected by the substitution in position 3 pattern. In the Δ8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but it is still comparable to that of amifostine.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina
VL  - 86
IS  - 9
SP  - 809
EP  - 817
DO  - 10.2298/JSC210401039S
ER  - 

@article{
author = "Sofrenić, Ivana V. and Anđelković, Boban D. and Vujisić, Ljubodrag V. and Novaković, Miroslav M. and Knežević, Aleksandar and Stanković, Miroslava
 and Milosavljević, Slobodan M. and Tešević, Vele",
year = "2021",
abstract = "Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested compounds showed a beneficial effect by reducing DNA damage of human lymphocytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in the concentration dependent manner, with the concentration of 2.0 µg mL-1 being the most effective – with increase of the concentration the activity slightly decreases. The structure–activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than Δ8-analogues. It was also demonstrated that the DNA protective activities within the Δ8-lanostane-26-oic acid group are affected by the substitution in position 3 pattern. In the Δ8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but it is still comparable to that of amifostine.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina",
volume = "86",
number = "9",
pages = "809-817",
doi = "10.2298/JSC210401039S"
}

Sofrenić, I. V., Anđelković, B. D., Vujisić, L. V., Novaković, M. M., Knežević, A., Stanković, M., Milosavljević, S. M.,& Tešević, V.. (2021). DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 86(9), 809-817.
https://doi.org/10.2298/JSC210401039S

Sofrenić IV, Anđelković BD, Vujisić LV, Novaković MM, Knežević A, Stanković M, Milosavljević SM, Tešević V. DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina. in Journal of the Serbian Chemical Society. 2021;86(9):809-817.
doi:10.2298/JSC210401039S .

Sofrenić, Ivana V., Anđelković, Boban D., Vujisić, Ljubodrag V., Novaković, Miroslav M., Knežević, Aleksandar, Stanković, Miroslava
, Milosavljević, Slobodan M., Tešević, Vele, "DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina" in Journal of the Serbian Chemical Society, 86, no. 9 (2021):809-817,
https://doi.org/10.2298/JSC210401039S . .

TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Stajić, Mirjana
AU  - Sofrenić, Ivana V.
AU  - Stanojković, Tatjana
AU  - Milovanović, Ivan
AU  - Tešević, Vele
AU  - Vukojević, Jelena
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2211
AB  - In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia
VL  - 13
IS  - 8
DO  - 10.1371/journal.pone.0203064
ER  - 

@article{
author = "Knežević, Aleksandar and Stajić, Mirjana and Sofrenić, Ivana V. and Stanojković, Tatjana and Milovanović, Ivan and Tešević, Vele and Vukojević, Jelena",
year = "2018",
abstract = "In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia",
volume = "13",
number = "8",
doi = "10.1371/journal.pone.0203064"
}

Knežević, A., Stajić, M., Sofrenić, I. V., Stanojković, T., Milovanović, I., Tešević, V.,& Vukojević, J.. (2018). Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One / Public Library of Science
Public Library Science, San Francisco., 13(8).
https://doi.org/10.1371/journal.pone.0203064

Knežević A, Stajić M, Sofrenić IV, Stanojković T, Milovanović I, Tešević V, Vukojević J. Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One / Public Library of Science. 2018;13(8).
doi:10.1371/journal.pone.0203064 .

Knežević, Aleksandar, Stajić, Mirjana, Sofrenić, Ivana V., Stanojković, Tatjana, Milovanović, Ivan, Tešević, Vele, Vukojević, Jelena, "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia" in PLoS One / Public Library of Science, 13, no. 8 (2018),
https://doi.org/10.1371/journal.pone.0203064 . .

TY  - DATA
AU  - Knežević, Aleksandar
AU  - Stajić, Mirjana
AU  - Sofrenić, Ivana V.
AU  - Stanojković, Tatjana
AU  - Milovanović, Ivan
AU  - Tešević, Vele
AU  - Vukojević, Jelena
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3169
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Supplementary material for the article: Knežević, A.; Stajić, M.; Sofrenić, I.; Stanojković, T.; Milovanović, I.; Tešević, V.; Vukojević, J. Antioxidative, Antifungal, Cytotoxic and Antineurodegenerative Activity of Selected Trametes Species from Serbia. PLoS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0203064
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3169
ER  - 

@misc{
author = "Knežević, Aleksandar and Stajić, Mirjana and Sofrenić, Ivana V. and Stanojković, Tatjana and Milovanović, Ivan and Tešević, Vele and Vukojević, Jelena",
year = "2018",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Supplementary material for the article: Knežević, A.; Stajić, M.; Sofrenić, I.; Stanojković, T.; Milovanović, I.; Tešević, V.; Vukojević, J. Antioxidative, Antifungal, Cytotoxic and Antineurodegenerative Activity of Selected Trametes Species from Serbia. PLoS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0203064",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3169"
}

Knežević, A., Stajić, M., Sofrenić, I. V., Stanojković, T., Milovanović, I., Tešević, V.,& Vukojević, J.. (2018). Supplementary material for the article: Knežević, A.; Stajić, M.; Sofrenić, I.; Stanojković, T.; Milovanović, I.; Tešević, V.; Vukojević, J. Antioxidative, Antifungal, Cytotoxic and Antineurodegenerative Activity of Selected Trametes Species from Serbia. PLoS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0203064. in PLoS One / Public Library of Science
Public Library Science, San Francisco..
https://hdl.handle.net/21.15107/rcub_cherry_3169

Knežević A, Stajić M, Sofrenić IV, Stanojković T, Milovanović I, Tešević V, Vukojević J. Supplementary material for the article: Knežević, A.; Stajić, M.; Sofrenić, I.; Stanojković, T.; Milovanović, I.; Tešević, V.; Vukojević, J. Antioxidative, Antifungal, Cytotoxic and Antineurodegenerative Activity of Selected Trametes Species from Serbia. PLoS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0203064. in PLoS One / Public Library of Science. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3169 .

Knežević, Aleksandar, Stajić, Mirjana, Sofrenić, Ivana V., Stanojković, Tatjana, Milovanović, Ivan, Tešević, Vele, Vukojević, Jelena, "Supplementary material for the article: Knežević, A.; Stajić, M.; Sofrenić, I.; Stanojković, T.; Milovanović, I.; Tešević, V.; Vukojević, J. Antioxidative, Antifungal, Cytotoxic and Antineurodegenerative Activity of Selected Trametes Species from Serbia. PLoS ONE 2018, 13 (8). https://doi.org/10.1371/journal.pone.0203064" in PLoS One / Public Library of Science (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3169 .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Stanojković, Tatjana
AU  - Stajić, Mirjana
AU  - Brčeski, Ilija
AU  - Knežević, Aleksandar
AU  - Ćilerdžić, Jasmina
AU  - Vukojević, Jelena
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1752
AB  - The aim of the study was an assessment of the antioxidant, antifungal and cytotoxic potentials of L. betulinus and T. hirsuta mycelia extracts and the effect of selenium on these activities. Extracts of L. betulinus were twice as efficient in DPPH center dot scavenging as those of T. hirsuta. The phenol content in Se-enriched L. betulinus extracts was higher than in non-enriched extracts, in contrast to the effect of Se-enrichment on T. hirsuta extracts, and a direct correlation between the amount and DPPH center dot scavenging effect was observed. Ethanol extracts exhibited fungistatic but not fungicidal activity against a range of micromycetes, and mycelium enrichment with selenium inhibited this effect. Although the extracts showed low cytotoxic activity against HeLa and LS174 cells, T. hirsuta extracts, especially those enriched with selenium, had better potential. L. betulinus extracts showed better antioxidant and antifungal activity than T. hirsuta extracts which were more active cytotoxic agents. The presence of selenium stimulated antioxidant and cytotoxic, and inhibited antifungal activity in L. betulinus, while in T. hirsuta its effect was slight.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Pharmaceutical Biotechnology
T1  - Effect of Selenium Enrichment of Lenzites betulinus and Trametes hirsuta Mycelia on Antioxidant, Antifungal and Cytostatics Potential
VL  - 16
IS  - 10
SP  - 920
EP  - 926
DO  - 10.2174/1389201016666150618152531
ER  - 

@article{
author = "Milovanović, Ivan and Stanojković, Tatjana and Stajić, Mirjana and Brčeski, Ilija and Knežević, Aleksandar and Ćilerdžić, Jasmina and Vukojević, Jelena",
year = "2015",
abstract = "The aim of the study was an assessment of the antioxidant, antifungal and cytotoxic potentials of L. betulinus and T. hirsuta mycelia extracts and the effect of selenium on these activities. Extracts of L. betulinus were twice as efficient in DPPH center dot scavenging as those of T. hirsuta. The phenol content in Se-enriched L. betulinus extracts was higher than in non-enriched extracts, in contrast to the effect of Se-enrichment on T. hirsuta extracts, and a direct correlation between the amount and DPPH center dot scavenging effect was observed. Ethanol extracts exhibited fungistatic but not fungicidal activity against a range of micromycetes, and mycelium enrichment with selenium inhibited this effect. Although the extracts showed low cytotoxic activity against HeLa and LS174 cells, T. hirsuta extracts, especially those enriched with selenium, had better potential. L. betulinus extracts showed better antioxidant and antifungal activity than T. hirsuta extracts which were more active cytotoxic agents. The presence of selenium stimulated antioxidant and cytotoxic, and inhibited antifungal activity in L. betulinus, while in T. hirsuta its effect was slight.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Pharmaceutical Biotechnology",
title = "Effect of Selenium Enrichment of Lenzites betulinus and Trametes hirsuta Mycelia on Antioxidant, Antifungal and Cytostatics Potential",
volume = "16",
number = "10",
pages = "920-926",
doi = "10.2174/1389201016666150618152531"
}

Milovanović, I., Stanojković, T., Stajić, M., Brčeski, I., Knežević, A., Ćilerdžić, J.,& Vukojević, J.. (2015). Effect of Selenium Enrichment of Lenzites betulinus and Trametes hirsuta Mycelia on Antioxidant, Antifungal and Cytostatics Potential. in Current Pharmaceutical Biotechnology
Bentham Science Publ Ltd, Sharjah., 16(10), 920-926.
https://doi.org/10.2174/1389201016666150618152531

Milovanović I, Stanojković T, Stajić M, Brčeski I, Knežević A, Ćilerdžić J, Vukojević J. Effect of Selenium Enrichment of Lenzites betulinus and Trametes hirsuta Mycelia on Antioxidant, Antifungal and Cytostatics Potential. in Current Pharmaceutical Biotechnology. 2015;16(10):920-926.
doi:10.2174/1389201016666150618152531 .

Milovanović, Ivan, Stanojković, Tatjana, Stajić, Mirjana, Brčeski, Ilija, Knežević, Aleksandar, Ćilerdžić, Jasmina, Vukojević, Jelena, "Effect of Selenium Enrichment of Lenzites betulinus and Trametes hirsuta Mycelia on Antioxidant, Antifungal and Cytostatics Potential" in Current Pharmaceutical Biotechnology, 16, no. 10 (2015):920-926,
https://doi.org/10.2174/1389201016666150618152531 . .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Brčeski, Ilija
AU  - Stajić, Mirjana
AU  - Korac, Aleksandra
AU  - Vukojević, Jelena
AU  - Knežević, Aleksandar
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1788
AB  - The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus. Mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg L-1 of Se, concentration of 500.0 mg L-1 strongly inhibited growth, and 1000.0 mg L-1 was the minimum inhibitory concentration. Contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at Se concentrations of 100.0 and 500.0 mg L-1, they were noticeably short, frequently septed and branched, with a more intensive extracellular matrix, and without clamp-connections. At high Se concentrations, hyphae with intact membrane, without cellular contents, with a high level of vacuolization, and with numerous proteinaceous bodies were observed. Biomass yield ranged between 11.8 g L-1, in the control, and 6.8 g L-1, at an Se concentration of 100.0 mg L-1, while no production was detected at a concentration of 500.0 mg L-1. Se content in the mycelia reached a peak (938.9 mu g g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L-1, while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L-1 Se.
PB  - Hindawi Publishing Corporation, New York
T2  - Scientific World Journal / TSWJ
T1  - Potential of Pleurotus ostreatus Mycelium for Selenium Absorption
DO  - 10.1155/2014/681834
ER  - 

@article{
author = "Milovanović, Ivan and Brčeski, Ilija and Stajić, Mirjana and Korac, Aleksandra and Vukojević, Jelena and Knežević, Aleksandar",
year = "2014",
abstract = "The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus. Mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg L-1 of Se, concentration of 500.0 mg L-1 strongly inhibited growth, and 1000.0 mg L-1 was the minimum inhibitory concentration. Contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at Se concentrations of 100.0 and 500.0 mg L-1, they were noticeably short, frequently septed and branched, with a more intensive extracellular matrix, and without clamp-connections. At high Se concentrations, hyphae with intact membrane, without cellular contents, with a high level of vacuolization, and with numerous proteinaceous bodies were observed. Biomass yield ranged between 11.8 g L-1, in the control, and 6.8 g L-1, at an Se concentration of 100.0 mg L-1, while no production was detected at a concentration of 500.0 mg L-1. Se content in the mycelia reached a peak (938.9 mu g g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L-1, while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L-1 Se.",
publisher = "Hindawi Publishing Corporation, New York",
journal = "Scientific World Journal / TSWJ",
title = "Potential of Pleurotus ostreatus Mycelium for Selenium Absorption",
doi = "10.1155/2014/681834"
}

Milovanović, I., Brčeski, I., Stajić, M., Korac, A., Vukojević, J.,& Knežević, A.. (2014). Potential of Pleurotus ostreatus Mycelium for Selenium Absorption. in Scientific World Journal / TSWJ
Hindawi Publishing Corporation, New York..
https://doi.org/10.1155/2014/681834

Milovanović I, Brčeski I, Stajić M, Korac A, Vukojević J, Knežević A. Potential of Pleurotus ostreatus Mycelium for Selenium Absorption. in Scientific World Journal / TSWJ. 2014;.
doi:10.1155/2014/681834 .

Milovanović, Ivan, Brčeski, Ilija, Stajić, Mirjana, Korac, Aleksandra, Vukojević, Jelena, Knežević, Aleksandar, "Potential of Pleurotus ostreatus Mycelium for Selenium Absorption" in Scientific World Journal / TSWJ (2014),
https://doi.org/10.1155/2014/681834 . .

TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Milovanović, Ivan
AU  - Stajić, Mirjana
AU  - Lončar, Nikola L.
AU  - Brčeski, Ilija
AU  - Vukojević, Jelena
AU  - Ćilerdžić, Jasmina
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1364
AB  - As biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. The goal of the study was to obtain clear insight into the dynamics of laccase, Mn-dependent peroxidase, and Mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. The best laccase producers were Pleurotus ostreatus and Pleurotus eryngii. Lenzites betulinus and Fomitopsis pinicola were the best Mn-dependent peroxidase producers, and P. ostreatus the weakest one. The peak of Mn-independent peroxidase was noted in Dichomytus squalens, and the minimum value in P. ostreatus. The profiles of the three enzymes, obtained by isoelectric focusing, were variable depending on the species and cultivation period. D. squalens was the best lignin degrader (34.1% of total lignin amount), and P. ostreatus and P. eryngii the weakest ones (7.1% and 14.5%, respectively).
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Lignin degradation by selected fungal species
VL  - 138
SP  - 117
EP  - 123
DO  - 10.1016/j.biortech.2013.03.182
ER  - 

@article{
author = "Knežević, Aleksandar and Milovanović, Ivan and Stajić, Mirjana and Lončar, Nikola L. and Brčeski, Ilija and Vukojević, Jelena and Ćilerdžić, Jasmina",
year = "2013",
abstract = "As biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. The goal of the study was to obtain clear insight into the dynamics of laccase, Mn-dependent peroxidase, and Mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. The best laccase producers were Pleurotus ostreatus and Pleurotus eryngii. Lenzites betulinus and Fomitopsis pinicola were the best Mn-dependent peroxidase producers, and P. ostreatus the weakest one. The peak of Mn-independent peroxidase was noted in Dichomytus squalens, and the minimum value in P. ostreatus. The profiles of the three enzymes, obtained by isoelectric focusing, were variable depending on the species and cultivation period. D. squalens was the best lignin degrader (34.1% of total lignin amount), and P. ostreatus and P. eryngii the weakest ones (7.1% and 14.5%, respectively).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Lignin degradation by selected fungal species",
volume = "138",
pages = "117-123",
doi = "10.1016/j.biortech.2013.03.182"
}

Knežević, A., Milovanović, I., Stajić, M., Lončar, N. L., Brčeski, I., Vukojević, J.,& Ćilerdžić, J.. (2013). Lignin degradation by selected fungal species. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 138, 117-123.
https://doi.org/10.1016/j.biortech.2013.03.182

Knežević A, Milovanović I, Stajić M, Lončar NL, Brčeski I, Vukojević J, Ćilerdžić J. Lignin degradation by selected fungal species. in Bioresource Technology. 2013;138:117-123.
doi:10.1016/j.biortech.2013.03.182 .

Knežević, Aleksandar, Milovanović, Ivan, Stajić, Mirjana, Lončar, Nikola L., Brčeski, Ilija, Vukojević, Jelena, Ćilerdžić, Jasmina, "Lignin degradation by selected fungal species" in Bioresource Technology, 138 (2013):117-123,
https://doi.org/10.1016/j.biortech.2013.03.182 . .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Brčeski, Ilija
AU  - Stajić, Mirjana
AU  - Knežević, Aleksandar
AU  - Vukojević, Jelena
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1439
AB  - The purpose of this study was to resolve the question of whether various selenium concentrations affect the ability of eight species, members of five genera, to produce mycelium biomass and absorb this trace element during submerged cultivation in Na2SeO3-enriched medium. The obtained results demonstrated the ability of mycelia of all of the tested species to absorb selenium at analyzed concentrations. Thus, selenium content ranged from 1.4 mu g g(-1) (Pleurotus eryngii) to 20.3 mu g g(-1) (Pleurotus ostreatus). The potential of mycelia to absorb selenium was significant, ranging from 8.1% (Lenzites betulinus) to 62.5% (P ostreatus) of its content in the medium. It may be concluded that all tested mushroom species could be used as satisfactory selenium sources due to the fact that the absorbed concentrations ranged from 15.8% (P eryngii) to 36.9% (P. ostreatus) of the dietary selenium amount (55 mu g d(-1)) recommended by the European Scientific Committee on Food.
PB  - Begell House Inc, Redding
T2  - International Journal of Medicinal Mushrooms
T1  - Potential Enrichment of Medicinal Mushrooms with Selenium to Obtain New Dietary Supplements
VL  - 15
IS  - 5
SP  - 449
EP  - 455
DO  - 10.1615/IntJMedMushr.v15.i5.30
ER  - 

@article{
author = "Milovanović, Ivan and Brčeski, Ilija and Stajić, Mirjana and Knežević, Aleksandar and Vukojević, Jelena",
year = "2013",
abstract = "The purpose of this study was to resolve the question of whether various selenium concentrations affect the ability of eight species, members of five genera, to produce mycelium biomass and absorb this trace element during submerged cultivation in Na2SeO3-enriched medium. The obtained results demonstrated the ability of mycelia of all of the tested species to absorb selenium at analyzed concentrations. Thus, selenium content ranged from 1.4 mu g g(-1) (Pleurotus eryngii) to 20.3 mu g g(-1) (Pleurotus ostreatus). The potential of mycelia to absorb selenium was significant, ranging from 8.1% (Lenzites betulinus) to 62.5% (P ostreatus) of its content in the medium. It may be concluded that all tested mushroom species could be used as satisfactory selenium sources due to the fact that the absorbed concentrations ranged from 15.8% (P eryngii) to 36.9% (P. ostreatus) of the dietary selenium amount (55 mu g d(-1)) recommended by the European Scientific Committee on Food.",
publisher = "Begell House Inc, Redding",
journal = "International Journal of Medicinal Mushrooms",
title = "Potential Enrichment of Medicinal Mushrooms with Selenium to Obtain New Dietary Supplements",
volume = "15",
number = "5",
pages = "449-455",
doi = "10.1615/IntJMedMushr.v15.i5.30"
}

Milovanović, I., Brčeski, I., Stajić, M., Knežević, A.,& Vukojević, J.. (2013). Potential Enrichment of Medicinal Mushrooms with Selenium to Obtain New Dietary Supplements. in International Journal of Medicinal Mushrooms
Begell House Inc, Redding., 15(5), 449-455.
https://doi.org/10.1615/IntJMedMushr.v15.i5.30

Milovanović I, Brčeski I, Stajić M, Knežević A, Vukojević J. Potential Enrichment of Medicinal Mushrooms with Selenium to Obtain New Dietary Supplements. in International Journal of Medicinal Mushrooms. 2013;15(5):449-455.
doi:10.1615/IntJMedMushr.v15.i5.30 .

Milovanović, Ivan, Brčeski, Ilija, Stajić, Mirjana, Knežević, Aleksandar, Vukojević, Jelena, "Potential Enrichment of Medicinal Mushrooms with Selenium to Obtain New Dietary Supplements" in International Journal of Medicinal Mushrooms, 15, no. 5 (2013):449-455,
https://doi.org/10.1615/IntJMedMushr.v15.i5.30 . .