TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Liang, Xinyue
AU  - Algarra, Manuel
AU  - Mi, Lan
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Kop, Tatjana
AU  - Bjelaković, Mira S.
AU  - Mitrović, Aleksandra D.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4268
AB  - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
VL  - 165
IS  - B
SP  - 2541
EP  - 2549
DO  - 10.1016/j.ijbiomac.2020.10.141
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana and Bjelaković, Mira S. and Mitrović, Aleksandra D. and Gojgić-Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana",
year = "2020",
abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives",
volume = "165",
number = "B",
pages = "2541-2549",
doi = "10.1016/j.ijbiomac.2020.10.141"
}

Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T., Bjelaković, M. S., Mitrović, A. D., Gojgić-Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules
Elsevier., 165(B), 2541-2549.
https://doi.org/10.1016/j.ijbiomac.2020.10.141

Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop T, Bjelaković MS, Mitrović AD, Gojgić-Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165(B):2541-2549.
doi:10.1016/j.ijbiomac.2020.10.141 .

Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana, Bjelaković, Mira S., Mitrović, Aleksandra D., Gojgić-Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165, no. B (2020):2541-2549,
https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3698
AB  - Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.
PB  - MDPI
T2  - Plants
T1  - Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development
VL  - 8
IS  - 11
DO  - 10.3390/plants8110478
ER  - 

@article{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
abstract = "Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.",
publisher = "MDPI",
journal = "Plants",
title = "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development",
volume = "8",
number = "11",
doi = "10.3390/plants8110478"
}

Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K.. (2019). Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development. in Plants
MDPI., 8(11).
https://doi.org/10.3390/plants8110478

Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development. in Plants. 2019;8(11).
doi:10.3390/plants8110478 .

Milenković, Ivana, Mitrović, Aleksandra D., Algarra, Manuel, Lázaro-Martínez, Juan M., Rodríguez-Castellón, Enrique, Maksimović, Vuk, Spasić, Slađana Z., Beškoski, Vladimir, Radotić, Ksenija, "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development" in Plants, 8, no. 11 (2019),
https://doi.org/10.3390/plants8110478 . .

TY  - DATA
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3699
PB  - MDPI
T2  - Plants
T1  - Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3699
ER  - 

@misc{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
publisher = "MDPI",
journal = "Plants",
title = "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3699"
}

Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K.. (2019). Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478. in Plants
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_3699

Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478. in Plants. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3699 .

Milenković, Ivana, Mitrović, Aleksandra D., Algarra, Manuel, Lázaro-Martínez, Juan M., Rodríguez-Castellón, Enrique, Maksimović, Vuk, Spasić, Slađana Z., Beškoski, Vladimir, Radotić, Ksenija, "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478" in Plants (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3699 .