TY  - JOUR
AU  - Pavlovic, R.
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Nenadovic, Vera
AU  - Ivanovic, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1300
AB  - The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae
VL  - 64
IS  - 2
SP  - 757
EP  - 765
DO  - 10.2298/ABS1202757P
ER  - 

@article{
author = "Pavlovic, R. and Grujić, Marica and Dojnov, Biljana and Vujčić, Miroslava and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran",
year = "2012",
abstract = "The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae",
volume = "64",
number = "2",
pages = "757-765",
doi = "10.2298/ABS1202757P"
}

Pavlovic, R., Grujić, M., Dojnov, B., Vujčić, M., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2012). Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 64(2), 757-765.
https://doi.org/10.2298/ABS1202757P

Pavlovic R, Grujić M, Dojnov B, Vujčić M, Nenadovic V, Ivanovic J, Vujčić Z. Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences. 2012;64(2):757-765.
doi:10.2298/ABS1202757P .

Pavlovic, R., Grujić, Marica, Dojnov, Biljana, Vujčić, Miroslava, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae" in Archives of biological sciences, 64, no. 2 (2012):757-765,
https://doi.org/10.2298/ABS1202757P . .