TY  - JOUR
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Šenerović, Lidija
AU  - Krivokapic, Nevena
AU  - Mitrovic, Tatjana
AU  - Bozovic, Nikola
AU  - Vasic, Vesna
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2373
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((5'2)-4'-methoxy-5'-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5'H-2,2'-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 

@article{
author = "Lazovic, Sasa and Leskovac, Andreja and Petrović, Sandra and Šenerović, Lidija and Krivokapic, Nevena and Mitrovic, Tatjana and Bozovic, Nikola and Vasic, Vesna and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((5'2)-4'-methoxy-5'-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5'H-2,2'-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}

Lazovic, S., Leskovac, A., Petrović, S., Šenerović, L., Krivokapic, N., Mitrovic, T., Bozovic, N., Vasic, V.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003

Lazovic S, Leskovac A, Petrović S, Šenerović L, Krivokapic N, Mitrovic T, Bozovic N, Vasic V, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .

Lazovic, Sasa, Leskovac, Andreja, Petrović, Sandra, Šenerović, Lidija, Krivokapic, Nevena, Mitrovic, Tatjana, Bozovic, Nikola, Vasic, Vesna, Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .

TY  - JOUR
AU  - Stamenković, S. M.
AU  - Ristić, Svetlana S.
AU  - Djekic, Tatjana L.
AU  - Mitrovic, Tatjana U.
AU  - Baošić, Rada
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1378
AB  - Air quality investigations have not been undertaken in Blace until now. Identifying the presence of different types of epiphytic lichens was performed in the summer 2012 in Blace (southeastern Serbia), and selected rural settlements around Blace, in order to establish the air quality of the area. The analysis of samples from described localities indicated the presence of 25 lichen taxa from 19 genera. Using the Index of Atmospheric Purity (IAP), it was found that there are 2 different air pollution zones in Blace: "lichen desert" and "transitional" or "struggle zone", which includes the periphery of the city. In these zones the air is moderately polluted. In the urban area of Blace there is no "normal" zone, but one was detected in the surrounding rural areas.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Air Quality Indication in Blace (Southeastern Serbia) Using Lichens as Bioindicators
VL  - 65
IS  - 3
SP  - 893
EP  - 897
DO  - 10.2298/ABS1303893S
ER  - 

@article{
author = "Stamenković, S. M. and Ristić, Svetlana S. and Djekic, Tatjana L. and Mitrovic, Tatjana U. and Baošić, Rada",
year = "2013",
abstract = "Air quality investigations have not been undertaken in Blace until now. Identifying the presence of different types of epiphytic lichens was performed in the summer 2012 in Blace (southeastern Serbia), and selected rural settlements around Blace, in order to establish the air quality of the area. The analysis of samples from described localities indicated the presence of 25 lichen taxa from 19 genera. Using the Index of Atmospheric Purity (IAP), it was found that there are 2 different air pollution zones in Blace: "lichen desert" and "transitional" or "struggle zone", which includes the periphery of the city. In these zones the air is moderately polluted. In the urban area of Blace there is no "normal" zone, but one was detected in the surrounding rural areas.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Air Quality Indication in Blace (Southeastern Serbia) Using Lichens as Bioindicators",
volume = "65",
number = "3",
pages = "893-897",
doi = "10.2298/ABS1303893S"
}

Stamenković, S. M., Ristić, S. S., Djekic, T. L., Mitrovic, T. U.,& Baošić, R.. (2013). Air Quality Indication in Blace (Southeastern Serbia) Using Lichens as Bioindicators. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 65(3), 893-897.
https://doi.org/10.2298/ABS1303893S

Stamenković SM, Ristić SS, Djekic TL, Mitrovic TU, Baošić R. Air Quality Indication in Blace (Southeastern Serbia) Using Lichens as Bioindicators. in Archives of biological sciences. 2013;65(3):893-897.
doi:10.2298/ABS1303893S .

Stamenković, S. M., Ristić, Svetlana S., Djekic, Tatjana L., Mitrovic, Tatjana U., Baošić, Rada, "Air Quality Indication in Blace (Southeastern Serbia) Using Lichens as Bioindicators" in Archives of biological sciences, 65, no. 3 (2013):893-897,
https://doi.org/10.2298/ABS1303893S . .

TY  - JOUR
AU  - Mitrovic, Tatjana
AU  - Stamenković, Slaviša
AU  - Cvetkovic, Vladimir
AU  - Nikolić, Miloš
AU  - Baošić, Rada
AU  - Mutić, Jelena
AU  - Anđelković, Tatjana
AU  - Bojic, Aleksandar
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1546
AB  - The widely spread lichen specie Flavoparmelia caperata was used in a biomonitoring study for atmospheric trace metal pollution in natural ecosystems in south-eastern Serbia. The concentration and distribution pattern of 21 metals in lichens were determined by inductively coupled plasma atomic emission spectrometry. The difference observed between metal deposition in the peripheral and central parts of lichen thalli reflected air quality changes in the last and previous years. These findings were confirmed with principal component analysis. The study demonstrated the accumulation of Ba, K, Mg, Na, Tl and Zn in the peripheral parts of thalli, while As, B, Cd, Cr, Cu, Fe, Ga, In, Li, Ni, Pb and Se were concentrated in the central parts of thalli.
AB  - Široko rasprostranjena vrsta lišajeva Flavoparmelia caperata je korišćena u biomonitoringu atmosferskog zagađenja metalima u tragovima u prirodnim ekosistemima na jugoistoku Srbije. Koncentracija i distribucija 21 metala u lišajevima je određena atomsko-emisionom spektrometrijom sa induktivno spregnutom plazmom. Primećena razlika u nagomilavanju metala između perifernih i centralnih delova talusa lišajeva je pripisana razlikama u kvalitetu vazduha poslednje i prethodnih godina. Ovi nalazi su potvrđeni analizom glavnih komponenti (PCA). Istraživanje je pokazalo akumuliranje Ba, K, Mg, Na, Tl i Zn u perifernim delovima talusa, odnosno As, B, Cd, Cr, Cu, Fe, Ga, In, Li, Ni, Pb i Se u centralnim delovima talusa.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Epiphytic lichen Flavoparmelia caperata as a sentinel for trace metal pollution
T1  - Epifitni lišaj Flavoparmelia caperata kao indikator zagađenja metalima u tragovima
VL  - 77
IS  - 9
SP  - 1301
EP  - 1310
DO  - 10.2298/JSC111124031M
ER  - 

@article{
author = "Mitrovic, Tatjana and Stamenković, Slaviša and Cvetkovic, Vladimir and Nikolić, Miloš and Baošić, Rada and Mutić, Jelena and Anđelković, Tatjana and Bojic, Aleksandar",
year = "2012",
abstract = "The widely spread lichen specie Flavoparmelia caperata was used in a biomonitoring study for atmospheric trace metal pollution in natural ecosystems in south-eastern Serbia. The concentration and distribution pattern of 21 metals in lichens were determined by inductively coupled plasma atomic emission spectrometry. The difference observed between metal deposition in the peripheral and central parts of lichen thalli reflected air quality changes in the last and previous years. These findings were confirmed with principal component analysis. The study demonstrated the accumulation of Ba, K, Mg, Na, Tl and Zn in the peripheral parts of thalli, while As, B, Cd, Cr, Cu, Fe, Ga, In, Li, Ni, Pb and Se were concentrated in the central parts of thalli., Široko rasprostranjena vrsta lišajeva Flavoparmelia caperata je korišćena u biomonitoringu atmosferskog zagađenja metalima u tragovima u prirodnim ekosistemima na jugoistoku Srbije. Koncentracija i distribucija 21 metala u lišajevima je određena atomsko-emisionom spektrometrijom sa induktivno spregnutom plazmom. Primećena razlika u nagomilavanju metala između perifernih i centralnih delova talusa lišajeva je pripisana razlikama u kvalitetu vazduha poslednje i prethodnih godina. Ovi nalazi su potvrđeni analizom glavnih komponenti (PCA). Istraživanje je pokazalo akumuliranje Ba, K, Mg, Na, Tl i Zn u perifernim delovima talusa, odnosno As, B, Cd, Cr, Cu, Fe, Ga, In, Li, Ni, Pb i Se u centralnim delovima talusa.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Epiphytic lichen Flavoparmelia caperata as a sentinel for trace metal pollution, Epifitni lišaj Flavoparmelia caperata kao indikator zagađenja metalima u tragovima",
volume = "77",
number = "9",
pages = "1301-1310",
doi = "10.2298/JSC111124031M"
}

Mitrovic, T., Stamenković, S., Cvetkovic, V., Nikolić, M., Baošić, R., Mutić, J., Anđelković, T.,& Bojic, A.. (2012). Epiphytic lichen Flavoparmelia caperata as a sentinel for trace metal pollution. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 77(9), 1301-1310.
https://doi.org/10.2298/JSC111124031M

Mitrovic T, Stamenković S, Cvetkovic V, Nikolić M, Baošić R, Mutić J, Anđelković T, Bojic A. Epiphytic lichen Flavoparmelia caperata as a sentinel for trace metal pollution. in Journal of the Serbian Chemical Society. 2012;77(9):1301-1310.
doi:10.2298/JSC111124031M .

Mitrovic, Tatjana, Stamenković, Slaviša, Cvetkovic, Vladimir, Nikolić, Miloš, Baošić, Rada, Mutić, Jelena, Anđelković, Tatjana, Bojic, Aleksandar, "Epiphytic lichen Flavoparmelia caperata as a sentinel for trace metal pollution" in Journal of the Serbian Chemical Society, 77, no. 9 (2012):1301-1310,
https://doi.org/10.2298/JSC111124031M . .