TY  - CONF
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Apostolović, Danijela
AU  - Cvetković, Anka
AU  - Veljković, Đorđe
AU  - Mutić, Jelena
AU  - van Hage, Marianne
AU  - Burazer, Lidija M.
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3737
PB  - Wiley
C3  - Allergy; Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI)
T1  - In- depth quantitative profiling of post- translational modifications of Timothy grass pollen allergome in relation to environmental pollution and oxidative stress
VL  - 74
IS  - supp. 106
SP  - 878
EP  - 878
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3737
ER  - 

@conference{
author = "Smiljanić, Katarina and Prodić, Ivana and Apostolović, Danijela and Cvetković, Anka and Veljković, Đorđe and Mutić, Jelena and van Hage, Marianne and Burazer, Lidija M. and Ćirković-Veličković, Tanja",
year = "2019",
publisher = "Wiley",
journal = "Allergy; Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI)",
title = "In- depth quantitative profiling of post- translational modifications of Timothy grass pollen allergome in relation to environmental pollution and oxidative stress",
volume = "74",
number = "supp. 106",
pages = "878-878",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3737"
}

Smiljanić, K., Prodić, I., Apostolović, D., Cvetković, A., Veljković, Đ., Mutić, J., van Hage, M., Burazer, L. M.,& Ćirković-Veličković, T.. (2019). In- depth quantitative profiling of post- translational modifications of Timothy grass pollen allergome in relation to environmental pollution and oxidative stress. in Allergy; Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI)
Wiley., 74(supp. 106), 878-878.
https://hdl.handle.net/21.15107/rcub_cherry_3737

Smiljanić K, Prodić I, Apostolović D, Cvetković A, Veljković Đ, Mutić J, van Hage M, Burazer LM, Ćirković-Veličković T. In- depth quantitative profiling of post- translational modifications of Timothy grass pollen allergome in relation to environmental pollution and oxidative stress. in Allergy; Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI). 2019;74(supp. 106):878-878.
https://hdl.handle.net/21.15107/rcub_cherry_3737 .

Smiljanić, Katarina, Prodić, Ivana, Apostolović, Danijela, Cvetković, Anka, Veljković, Đorđe, Mutić, Jelena, van Hage, Marianne, Burazer, Lidija M., Ćirković-Veličković, Tanja, "In- depth quantitative profiling of post- translational modifications of Timothy grass pollen allergome in relation to environmental pollution and oxidative stress" in Allergy; Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI), 74, no. supp. 106 (2019):878-878,
https://hdl.handle.net/21.15107/rcub_cherry_3737 .

TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Apostolović, Danijela
AU  - Cvetković, Anka
AU  - Veljović, Đorđe
AU  - Mutić, Jelena
AU  - van Hage, Marianne
AU  - Burazer, Lidija M.
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2857
AB  - An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.
PB  - Elsevier
T2  - Environment International
T1  - In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress
VL  - 126
SP  - 644
EP  - 658
DO  - 10.1016/j.envint.2019.03.001
ER  - 

@article{
author = "Smiljanić, Katarina and Prodić, Ivana and Apostolović, Danijela and Cvetković, Anka and Veljović, Đorđe and Mutić, Jelena and van Hage, Marianne and Burazer, Lidija M. and Ćirković-Veličković, Tanja",
year = "2019",
abstract = "An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.",
publisher = "Elsevier",
journal = "Environment International",
title = "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress",
volume = "126",
pages = "644-658",
doi = "10.1016/j.envint.2019.03.001"
}

Smiljanić, K., Prodić, I., Apostolović, D., Cvetković, A., Veljović, Đ., Mutić, J., van Hage, M., Burazer, L. M.,& Ćirković-Veličković, T.. (2019). In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International
Elsevier., 126, 644-658.
https://doi.org/10.1016/j.envint.2019.03.001

Smiljanić K, Prodić I, Apostolović D, Cvetković A, Veljović Đ, Mutić J, van Hage M, Burazer LM, Ćirković-Veličković T. In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International. 2019;126:644-658.
doi:10.1016/j.envint.2019.03.001 .

Smiljanić, Katarina, Prodić, Ivana, Apostolović, Danijela, Cvetković, Anka, Veljović, Đorđe, Mutić, Jelena, van Hage, Marianne, Burazer, Lidija M., Ćirković-Veličković, Tanja, "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress" in Environment International, 126 (2019):644-658,
https://doi.org/10.1016/j.envint.2019.03.001 . .

TY  - JOUR
AU  - Živković, Marija
AU  - Jovasevic-Stojanovic, Milena
AU  - Cvetković, Anka
AU  - Lazovic, Ivan
AU  - Tasic, Visa
AU  - Stevanovic, Zana
AU  - Gržetić, Ivan
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1716
AB  - This study investigated seasonal variation of PAHs and their partition between gas and particulate-bounded phases in indoor and outdoor air in 4 schools In Serbia located at different locations. The sampling campaigns were conducted during one workweek at each school successively. Campaigns were conducted in schools during heating and non-heating seasons in December 2011 and June 2012. Seasonal variations of gas and particle-bounded PAHs concentrations were observed with higher levels during heating season. The highest total PAH values were associated with the gas phase in both sampling periods. The total PAHs concentration at indoor and at the outdoor sites, during heating season, ranged from 88.45 to 447.72 ng/m(3) and 201.69 to 1017.15 ng/m(3), respectively. During non-heating season, the total PAHs concentration ranged from 36.91 to 271.57 ng/m(3) in Indoor environment and 27.00 to 132.32 ng/m(3) in outdoor environment. Most of the I/O ratios were less than 1, which indicated that the indoor PAHs were mostly from outdoor sources. The use of diagnostic ratio showed that traffic emission and coal combustion are the major sources of PAHs. Only the diagnostic ratios for the school located near the industrial area showed significant deviation compared to other schools.
AB  - U ovom radu su istraživane sezonske promene PAH i njihova raspodela, u gasnoj fazi i respirabilnim česticama u vazduhu unutrašnjeg prostora i spoljašnje sredine u 4 škole u Srbiji koje se nalaze na različitim lokacijama. Kampanja uzorkovanja je sprovedena tokom jedne radne nedelje u toku grejnog i negrejnog perioda (od decembra 2011. do juna 2012.) u svakoj školi. Uočene su sezonske promene nivoa PAH u gasnoj fazi i česticama, sa višim koncentracijama tokom grejnog perioda. Izmerena je veća vrednost zbira ukupnih PAH tokom oba perioda u gasnoj fazi nego PAH u česticama. Ukupna koncentracija PAH tokom grejnog perioda u unutrašnjem prostoru se kretala u opsegu od 88,45 do 447,72 ng/m3, a u spoljašnjoj sredini od 201,69 do 1017,15 ng/m3. Tokom negrejnog perioda, ukupna koncentracija PAH-ova je bila 36,91-271,57 ng/m3 u unutrašnjem prostoru, dok je u spoljašnjoj sredini bila 27,00-132,32 ng/m3. Većina I/O odnosa je bila manja od 1, što ukazuje da PAH u unutrašnjem prostoru uglavnom potiču iz spoljašnjeg vazduha. Na osnovu dijagnostičkih odnosa zaključeno je da su saobraćaj i sagorevanje uglja glavni izvori PAH. Jedino dijagnostički odnosi za školu koja se nalazi u blizini industrijske zone pokazuju značajno odstupanje u poređenju sa drugim školama.
PB  - Assoc Chemical Eng, Belgrade
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Pahs Levels in Gas and Particle-Bound Phase in Schools at Different Locations in Serbia
T1  - Nivo policikličnih aromatičnih ugljovodonika u gasnoj i čestičnoj fazi u školama na različitim lokacijama u Srbiji
VL  - 21
IS  - 1
SP  - 159
EP  - 167
DO  - 10.2298/CICEQ140206016Z
ER  - 

@article{
author = "Živković, Marija and Jovasevic-Stojanovic, Milena and Cvetković, Anka and Lazovic, Ivan and Tasic, Visa and Stevanovic, Zana and Gržetić, Ivan",
year = "2015",
abstract = "This study investigated seasonal variation of PAHs and their partition between gas and particulate-bounded phases in indoor and outdoor air in 4 schools In Serbia located at different locations. The sampling campaigns were conducted during one workweek at each school successively. Campaigns were conducted in schools during heating and non-heating seasons in December 2011 and June 2012. Seasonal variations of gas and particle-bounded PAHs concentrations were observed with higher levels during heating season. The highest total PAH values were associated with the gas phase in both sampling periods. The total PAHs concentration at indoor and at the outdoor sites, during heating season, ranged from 88.45 to 447.72 ng/m(3) and 201.69 to 1017.15 ng/m(3), respectively. During non-heating season, the total PAHs concentration ranged from 36.91 to 271.57 ng/m(3) in Indoor environment and 27.00 to 132.32 ng/m(3) in outdoor environment. Most of the I/O ratios were less than 1, which indicated that the indoor PAHs were mostly from outdoor sources. The use of diagnostic ratio showed that traffic emission and coal combustion are the major sources of PAHs. Only the diagnostic ratios for the school located near the industrial area showed significant deviation compared to other schools., U ovom radu su istraživane sezonske promene PAH i njihova raspodela, u gasnoj fazi i respirabilnim česticama u vazduhu unutrašnjeg prostora i spoljašnje sredine u 4 škole u Srbiji koje se nalaze na različitim lokacijama. Kampanja uzorkovanja je sprovedena tokom jedne radne nedelje u toku grejnog i negrejnog perioda (od decembra 2011. do juna 2012.) u svakoj školi. Uočene su sezonske promene nivoa PAH u gasnoj fazi i česticama, sa višim koncentracijama tokom grejnog perioda. Izmerena je veća vrednost zbira ukupnih PAH tokom oba perioda u gasnoj fazi nego PAH u česticama. Ukupna koncentracija PAH tokom grejnog perioda u unutrašnjem prostoru se kretala u opsegu od 88,45 do 447,72 ng/m3, a u spoljašnjoj sredini od 201,69 do 1017,15 ng/m3. Tokom negrejnog perioda, ukupna koncentracija PAH-ova je bila 36,91-271,57 ng/m3 u unutrašnjem prostoru, dok je u spoljašnjoj sredini bila 27,00-132,32 ng/m3. Većina I/O odnosa je bila manja od 1, što ukazuje da PAH u unutrašnjem prostoru uglavnom potiču iz spoljašnjeg vazduha. Na osnovu dijagnostičkih odnosa zaključeno je da su saobraćaj i sagorevanje uglja glavni izvori PAH. Jedino dijagnostički odnosi za školu koja se nalazi u blizini industrijske zone pokazuju značajno odstupanje u poređenju sa drugim školama.",
publisher = "Assoc Chemical Eng, Belgrade",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Pahs Levels in Gas and Particle-Bound Phase in Schools at Different Locations in Serbia, Nivo policikličnih aromatičnih ugljovodonika u gasnoj i čestičnoj fazi u školama na različitim lokacijama u Srbiji",
volume = "21",
number = "1",
pages = "159-167",
doi = "10.2298/CICEQ140206016Z"
}

Živković, M., Jovasevic-Stojanovic, M., Cvetković, A., Lazovic, I., Tasic, V., Stevanovic, Z.,& Gržetić, I.. (2015). Pahs Levels in Gas and Particle-Bound Phase in Schools at Different Locations in Serbia. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Assoc Chemical Eng, Belgrade., 21(1), 159-167.
https://doi.org/10.2298/CICEQ140206016Z

Živković M, Jovasevic-Stojanovic M, Cvetković A, Lazovic I, Tasic V, Stevanovic Z, Gržetić I. Pahs Levels in Gas and Particle-Bound Phase in Schools at Different Locations in Serbia. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2015;21(1):159-167.
doi:10.2298/CICEQ140206016Z .

Živković, Marija, Jovasevic-Stojanovic, Milena, Cvetković, Anka, Lazovic, Ivan, Tasic, Visa, Stevanovic, Zana, Gržetić, Ivan, "Pahs Levels in Gas and Particle-Bound Phase in Schools at Different Locations in Serbia" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 21, no. 1 (2015):159-167,
https://doi.org/10.2298/CICEQ140206016Z . .