TY  - JOUR
AU  - Rašković, Brankica
AU  - Polović, Natalija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2268
AB  - Ficus carica, the common fig has been widely used for centuries for medicine as well as food. Fig latex is used for the treatments of cancer, inflammatory diseases, bacterial and gastrointestinal nematode infections, warts and skin diseases. The aim of the study was to investigate if some of the fig latex applications could be attributed to collagenase activity. The usage of figs in the Western Balkans indicated that the latex of unripe fruits is often used for treatments that could involve remodeling of connective tissue. Collagenase activity in fig latices collected in spring was twice as high as the one detected in summer. Collagenase improved diffusion of low-molecular-weight model molecule through the gelatin hydrogel. Fig latex collagenase was stable during boiling and in the simulated gastric conditions for up to 1 h. The presence of fig latex collagenase in traditional medicine preparations could increase treatment efficacy by hydrolyzing collagen present in extracellular matrix and facilitating the penetration of active molecules through the connective tissue. (C) 2016 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Herbal Medicine
T1  - Collegenase activity in fig latex could contribute to its efficacy in ethnomedicinal preparations
VL  - 6
IS  - 2
SP  - 73
EP  - 78
DO  - 10.1016/j.hermed.2016.03.002
ER  - 

@article{
author = "Rašković, Brankica and Polović, Natalija",
year = "2016",
abstract = "Ficus carica, the common fig has been widely used for centuries for medicine as well as food. Fig latex is used for the treatments of cancer, inflammatory diseases, bacterial and gastrointestinal nematode infections, warts and skin diseases. The aim of the study was to investigate if some of the fig latex applications could be attributed to collagenase activity. The usage of figs in the Western Balkans indicated that the latex of unripe fruits is often used for treatments that could involve remodeling of connective tissue. Collagenase activity in fig latices collected in spring was twice as high as the one detected in summer. Collagenase improved diffusion of low-molecular-weight model molecule through the gelatin hydrogel. Fig latex collagenase was stable during boiling and in the simulated gastric conditions for up to 1 h. The presence of fig latex collagenase in traditional medicine preparations could increase treatment efficacy by hydrolyzing collagen present in extracellular matrix and facilitating the penetration of active molecules through the connective tissue. (C) 2016 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Herbal Medicine",
title = "Collegenase activity in fig latex could contribute to its efficacy in ethnomedicinal preparations",
volume = "6",
number = "2",
pages = "73-78",
doi = "10.1016/j.hermed.2016.03.002"
}

Rašković, B.,& Polović, N.. (2016). Collegenase activity in fig latex could contribute to its efficacy in ethnomedicinal preparations. in Journal of Herbal Medicine
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 6(2), 73-78.
https://doi.org/10.1016/j.hermed.2016.03.002

Rašković B, Polović N. Collegenase activity in fig latex could contribute to its efficacy in ethnomedicinal preparations. in Journal of Herbal Medicine. 2016;6(2):73-78.
doi:10.1016/j.hermed.2016.03.002 .

Rašković, Brankica, Polović, Natalija, "Collegenase activity in fig latex could contribute to its efficacy in ethnomedicinal preparations" in Journal of Herbal Medicine, 6, no. 2 (2016):73-78,
https://doi.org/10.1016/j.hermed.2016.03.002 . .

TY  - JOUR
AU  - Rašković, Brankica
AU  - Lazić, Jelena O.
AU  - Polović, Natalija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3397
AB  - The physiological role of fig latex is to protect the plant from pathogens. Latex is a rich source of proteases, predominantly ficin. Fig latex also contains collagenolytic protease and chitinolytic enzymes. Our aim was to investigate changes in protein composition, enzyme and antifungal activities of fig latex during fruit ripening. RESULTSComparison of latex samples in different time periods showed a uniform increase of protein concentration in chronological order. The content of collagenolytic protease did not differ significantly in the latex samples, while the content of ficin decreased. Ficin-specific activity towards casein was the highest at the beginning of fruit development (about 80 U mg(-1)). Specific milk clotting activity increased as well as the abundance of casein band in the clots. Specific chitinolytic activity at the beginning of flowering was 6.5 times higher than the activity in the period when fruits are ripe. Antifungal activity is the most extensive in spring. CONCLUSIONFicin forms with different casein specificities are present in different proportions during fruit ripening, which is of importance for applications in the dairy industry. The protection mechanism against insects and fungi, which relies on chitinolytic activity, is the most important in the early phases of flowering and is replaced with other strategies over time. (c) 2015 Society of Chemical Industry
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening
VL  - 96
IS  - 2
SP  - 576
EP  - 582
DO  - 10.1002/jsfa.7126
ER  - 

@article{
author = "Rašković, Brankica and Lazić, Jelena O. and Polović, Natalija",
year = "2016",
abstract = "The physiological role of fig latex is to protect the plant from pathogens. Latex is a rich source of proteases, predominantly ficin. Fig latex also contains collagenolytic protease and chitinolytic enzymes. Our aim was to investigate changes in protein composition, enzyme and antifungal activities of fig latex during fruit ripening. RESULTSComparison of latex samples in different time periods showed a uniform increase of protein concentration in chronological order. The content of collagenolytic protease did not differ significantly in the latex samples, while the content of ficin decreased. Ficin-specific activity towards casein was the highest at the beginning of fruit development (about 80 U mg(-1)). Specific milk clotting activity increased as well as the abundance of casein band in the clots. Specific chitinolytic activity at the beginning of flowering was 6.5 times higher than the activity in the period when fruits are ripe. Antifungal activity is the most extensive in spring. CONCLUSIONFicin forms with different casein specificities are present in different proportions during fruit ripening, which is of importance for applications in the dairy industry. The protection mechanism against insects and fungi, which relies on chitinolytic activity, is the most important in the early phases of flowering and is replaced with other strategies over time. (c) 2015 Society of Chemical Industry",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening",
volume = "96",
number = "2",
pages = "576-582",
doi = "10.1002/jsfa.7126"
}

Rašković, B., Lazić, J. O.,& Polović, N.. (2016). Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken., 96(2), 576-582.
https://doi.org/10.1002/jsfa.7126

Rašković B, Lazić JO, Polović N. Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening. in Journal of the Science of Food and Agriculture. 2016;96(2):576-582.
doi:10.1002/jsfa.7126 .

Rašković, Brankica, Lazić, Jelena O., Polović, Natalija, "Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening" in Journal of the Science of Food and Agriculture, 96, no. 2 (2016):576-582,
https://doi.org/10.1002/jsfa.7126 . .

TY  - DATA
AU  - Rašković, Brankica
AU  - Lazić, Jelena O.
AU  - Polović, Natalija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3398
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Supplementary data for the article: Raskovic, B.; Lazic, J.; Polovic, N. Characterisation of General Proteolytic, Milk Clotting and Antifungal Activity of Ficus Carica Latex during Fruit Ripening. Journal of the Science of Food and Agriculture 2016, 96 (2), 576–582. https://doi.org/10.1002/jsfa.7126
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3398
ER  - 

@misc{
author = "Rašković, Brankica and Lazić, Jelena O. and Polović, Natalija",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Supplementary data for the article: Raskovic, B.; Lazic, J.; Polovic, N. Characterisation of General Proteolytic, Milk Clotting and Antifungal Activity of Ficus Carica Latex during Fruit Ripening. Journal of the Science of Food and Agriculture 2016, 96 (2), 576–582. https://doi.org/10.1002/jsfa.7126",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3398"
}

Rašković, B., Lazić, J. O.,& Polović, N.. (2016). Supplementary data for the article: Raskovic, B.; Lazic, J.; Polovic, N. Characterisation of General Proteolytic, Milk Clotting and Antifungal Activity of Ficus Carica Latex during Fruit Ripening. Journal of the Science of Food and Agriculture 2016, 96 (2), 576–582. https://doi.org/10.1002/jsfa.7126. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3398

Rašković B, Lazić JO, Polović N. Supplementary data for the article: Raskovic, B.; Lazic, J.; Polovic, N. Characterisation of General Proteolytic, Milk Clotting and Antifungal Activity of Ficus Carica Latex during Fruit Ripening. Journal of the Science of Food and Agriculture 2016, 96 (2), 576–582. https://doi.org/10.1002/jsfa.7126. in Journal of the Science of Food and Agriculture. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3398 .

Rašković, Brankica, Lazić, Jelena O., Polović, Natalija, "Supplementary data for the article: Raskovic, B.; Lazic, J.; Polovic, N. Characterisation of General Proteolytic, Milk Clotting and Antifungal Activity of Ficus Carica Latex during Fruit Ripening. Journal of the Science of Food and Agriculture 2016, 96 (2), 576–582. https://doi.org/10.1002/jsfa.7126" in Journal of the Science of Food and Agriculture (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3398 .