TY  - CONF
AU  - Smiljanić, Katarina
AU  - Al Hanish, Aya
AU  - Trifunovic, Sara
AU  - Perusko, Marija
AU  - Mihajlovic, Luka
AU  - Ognjenovic, Jana
AU  - Ćirković-Veličković, Tanja
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6453
AB  - Aims and scopes: It is known that sub-pollen particles (SPP) cause more severe symptoms of asthma than its whole pollen grain counterparts, 
due to its smaller size and ability to penetrate deeper into the lungs. To reveal other possible causes of such more severe asthma symptoms 
induced by Ambrosia artemisiifolia SPP, its sub-pollen particle and pollen grain proteomes were characterized and compared. 
Experimental description: Protein extract of short ragweed (Ambrosia artemisiifolia) pollen and its SPP were prepared and subjected to 
denaturing 2-D electrophoresis. Pollen proteome spots were excised a er colloidal coomassie blue brillinat (cCBB) staining and in gel digested 
for liquid chromatography coupled with high resolution LTQ Orbitrap XL hybrid mass spectrometry. Parallel to that, cCBB stained gels were 
analyzed and quanti ed with laser scanner Typhoon 7000 series and Image 2D Master Platinum 7.0 soware (GE Healthcare, USA).  
Results: 
ere is statistically signi cant di erence between the contents of major allergen Amb a 1.05 subgroup in the ragweed whole 
pollen grains and SPP, the latter being richer in Amb a 1.05 (2 times), in major allergen Amb a 11 (5 times), in minor allergens Amb a 4 (7 
times) and Amb a 6 (4 times). e 30 kDa basic antigen group in SPP (8 times more abundant) needs further investigation.
sia artemisiifolia allergens, Amb a 1.05 and Amb a 11, minor allergens Amb a 4 and Amb a 6 which could contribute to more severe asthma 
symptoms caused by SPP.
PB  - Portugal : BIOSCOPE Research Group
PB  - Portugal : PROTEOMASS Scientific Society
C3  - Journal of Integrated OMICS
T1  - Comparison of 2D proteomic maps revealed properties of Ambrosia artemisiifolia sub-pollen particles accounting for more severe asthma symptoms than its whole pollen grains
VL  - 5
IS  - ‚2
SP  - 34
EP  - 35
DO  - 10.5584/jiomics.v5i2.192
ER  - 

@conference{
author = "Smiljanić, Katarina and Al Hanish, Aya and Trifunovic, Sara and Perusko, Marija and Mihajlovic, Luka and Ognjenovic, Jana and Ćirković-Veličković, Tanja",
year = "2015",
abstract = "Aims and scopes: It is known that sub-pollen particles (SPP) cause more severe symptoms of asthma than its whole pollen grain counterparts, 
due to its smaller size and ability to penetrate deeper into the lungs. To reveal other possible causes of such more severe asthma symptoms 
induced by Ambrosia artemisiifolia SPP, its sub-pollen particle and pollen grain proteomes were characterized and compared. 
Experimental description: Protein extract of short ragweed (Ambrosia artemisiifolia) pollen and its SPP were prepared and subjected to 
denaturing 2-D electrophoresis. Pollen proteome spots were excised a er colloidal coomassie blue brillinat (cCBB) staining and in gel digested 
for liquid chromatography coupled with high resolution LTQ Orbitrap XL hybrid mass spectrometry. Parallel to that, cCBB stained gels were 
analyzed and quanti ed with laser scanner Typhoon 7000 series and Image 2D Master Platinum 7.0 soware (GE Healthcare, USA).  
Results: 
ere is statistically signi cant di erence between the contents of major allergen Amb a 1.05 subgroup in the ragweed whole 
pollen grains and SPP, the latter being richer in Amb a 1.05 (2 times), in major allergen Amb a 11 (5 times), in minor allergens Amb a 4 (7 
times) and Amb a 6 (4 times). e 30 kDa basic antigen group in SPP (8 times more abundant) needs further investigation.
sia artemisiifolia allergens, Amb a 1.05 and Amb a 11, minor allergens Amb a 4 and Amb a 6 which could contribute to more severe asthma 
symptoms caused by SPP.",
publisher = "Portugal : BIOSCOPE Research Group, Portugal : PROTEOMASS Scientific Society",
journal = "Journal of Integrated OMICS",
title = "Comparison of 2D proteomic maps revealed properties of Ambrosia artemisiifolia sub-pollen particles accounting for more severe asthma symptoms than its whole pollen grains",
volume = "5",
number = "‚2",
pages = "34-35",
doi = "10.5584/jiomics.v5i2.192"
}

Smiljanić, K., Al Hanish, A., Trifunovic, S., Perusko, M., Mihajlovic, L., Ognjenovic, J.,& Ćirković-Veličković, T.. (2015). Comparison of 2D proteomic maps revealed properties of Ambrosia artemisiifolia sub-pollen particles accounting for more severe asthma symptoms than its whole pollen grains. in Journal of Integrated OMICS
Portugal : BIOSCOPE Research Group., 5(‚2), 34-35.
https://doi.org/10.5584/jiomics.v5i2.192

Smiljanić K, Al Hanish A, Trifunovic S, Perusko M, Mihajlovic L, Ognjenovic J, Ćirković-Veličković T. Comparison of 2D proteomic maps revealed properties of Ambrosia artemisiifolia sub-pollen particles accounting for more severe asthma symptoms than its whole pollen grains. in Journal of Integrated OMICS. 2015;5(‚2):34-35.
doi:10.5584/jiomics.v5i2.192 .

Smiljanić, Katarina, Al Hanish, Aya, Trifunovic, Sara, Perusko, Marija, Mihajlovic, Luka, Ognjenovic, Jana, Ćirković-Veličković, Tanja, "Comparison of 2D proteomic maps revealed properties of Ambrosia artemisiifolia sub-pollen particles accounting for more severe asthma symptoms than its whole pollen grains" in Journal of Integrated OMICS, 5, no. ‚2 (2015):34-35,
https://doi.org/10.5584/jiomics.v5i2.192 . .