TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baošić, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5268
AB  - Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study
VL  - 102
IS  - 6
SP  - 2500
EP  - 2505
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baošić, Rada and Radotić, Ksenija",
year = "2022",
abstract = "Aflatoxin B1 (AFB1), is a naturally hazardous environmental contaminant, contained mainly in various cereal seeds. The maize (Zea mays L.) seeds containing various levels of the AFB1 was investigated using optical and electron paramagnetic resonance spectroscopies. The changes of lignin content and organic free radicals as indicators of the seeds' defense response to the AFB1-stressor were estimated, in the seeds' inner and outer fractions. The lignin content was determined using the Acetyl Bromide method. No correlation was found between the lignin content and the AFB1 concentration in the inner fraction (R of 0.030, p = 0.943), while a significant positive linear correlation (R of 0.9923, p= 0.00005) between the lignin content and the AFB1 concentration in the outer fraction was obtained. Employing electron paramagnetic resonance (EPR) spectroscopy, the different response of the fractions was observed, regarding the content of free radicals with increasing AFB1 concentrations. In addition, front-face fluorescence spectroscopy in combination with the deconvolution showed a positive linear correlation between the ratio of the green and blue spectral emission components (C4/C2) area and the AFB1 concentration in the outer fraction. Thus lignin content and the (C4/C2) area ratio may be reliable indicators for the screening of lignin changes related to the level of AFB1 in the seeds.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study",
volume = "102",
number = "6",
pages = "2500-2505",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baošić, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baošić R, Radotić K. Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baošić, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays  L.) seeds in response to aflatoxin B1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - JOUR
AU  - Laketa, Danijela
AU  - Bogdanović, Jelena
AU  - Prodanović, Radivoje
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1089
AB  - We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model
VL  - 29
IS  - 2
SP  - 122
EP  - 128
DO  - 10.4149/gpb_2010_02_122
ER  - 

@article{
author = "Laketa, Danijela and Bogdanović, Jelena and Prodanović, Radivoje and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2010",
abstract = "We studied the dependence of peroxidase (POD) activity on pH in crude extract of Picea omorika (Pane.) Purkinye needles and in its acidic and basic fractions, obtained by ion exchange chromatography. Nonlinear regression was applied on the activity data with pH as the explaining variable, using the Levenberg-Marquardt algorithm. Studying crude extract at three different temperatures, the shape of the simulated activity/pH dependences indicated an existence of two components, which was confirmed by mathematical modeling. The kinetic parameters Act(0) K(EH) and K(EOH) of both components are presented. The curves and pH optima shifted under increasing temperatures towards lower pH values, which was verified after decomposition. Nonlinear regression detected the presence of two components for both fractions, and there is no considerable difference between their pH optima. Our results show for the first time that the sum of components, each described by the mathematical model employed, can be used to explain the complex pH-related POD activity in the extract with two or more enzyme forms simultaneously active.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model",
volume = "29",
number = "2",
pages = "122-128",
doi = "10.4149/gpb_2010_02_122"
}

Laketa, D., Bogdanović, J., Prodanović, R., Kalauzi, A.,& Radotić, K.. (2010). The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 29(2), 122-128.
https://doi.org/10.4149/gpb_2010_02_122

Laketa D, Bogdanović J, Prodanović R, Kalauzi A, Radotić K. The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model. in General Physiology and Biophysics. 2010;29(2):122-128.
doi:10.4149/gpb_2010_02_122 .

Laketa, Danijela, Bogdanović, Jelena, Prodanović, Radivoje, Kalauzi, Aleksandar, Radotić, Ksenija, "The effect of pH on the activity of soluble peroxidase in needles of Serbian spruce (Picea omorika (Panc.) Purkinye): application of a mathematical model" in General Physiology and Biophysics, 29, no. 2 (2010):122-128,
https://doi.org/10.4149/gpb_2010_02_122 . .