TY  - JOUR
AU  - Nikolić, Milan
AU  - Medic, Danijela Ristic
AU  - Stanić-Vučinić, Dragana
AU  - Postic, Marija
AU  - Arsic, Aleksandra
AU  - Niketić, Vesna
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/936
AB  - Background Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males. Aim of the study To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes. Methods Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol  lt  3.4 mmol/l and total cholesterol  lt  5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol  gt = 3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat ( lt = 30% total energy as fat) and high-fat consumers ( gt  30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjects with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration. Results In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P  lt  0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P  lt  0.001) in subjects with high Hb-Ch at onset. Conclusion High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes.
PB  - Springer Heidelberg, Heidelberg
T2  - European Journal of Nutrition
T1  - Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes
VL  - 47
IS  - 3
SP  - 123
EP  - 130
DO  - 10.1007/s00394-008-0705-z
ER  - 

@article{
author = "Nikolić, Milan and Medic, Danijela Ristic and Stanić-Vučinić, Dragana and Postic, Marija and Arsic, Aleksandra and Niketić, Vesna",
year = "2008",
abstract = "Background Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males. Aim of the study To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes. Methods Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol  lt  3.4 mmol/l and total cholesterol  lt  5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol  gt = 3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat ( lt = 30% total energy as fat) and high-fat consumers ( gt  30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjects with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration. Results In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P  lt  0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P  lt  0.001) in subjects with high Hb-Ch at onset. Conclusion High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "European Journal of Nutrition",
title = "Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes",
volume = "47",
number = "3",
pages = "123-130",
doi = "10.1007/s00394-008-0705-z"
}

Nikolić, M., Medic, D. R., Stanić-Vučinić, D., Postic, M., Arsic, A.,& Niketić, V.. (2008). Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes. in European Journal of Nutrition
Springer Heidelberg, Heidelberg., 47(3), 123-130.
https://doi.org/10.1007/s00394-008-0705-z

Nikolić M, Medic DR, Stanić-Vučinić D, Postic M, Arsic A, Niketić V. Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes. in European Journal of Nutrition. 2008;47(3):123-130.
doi:10.1007/s00394-008-0705-z .

Nikolić, Milan, Medic, Danijela Ristic, Stanić-Vučinić, Dragana, Postic, Marija, Arsic, Aleksandra, Niketić, Vesna, "Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes" in European Journal of Nutrition, 47, no. 3 (2008):123-130,
https://doi.org/10.1007/s00394-008-0705-z . .