Krstic, Branka

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In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit

Popović, Milica M.; Bulajic, Aleksandra; Ristic, Danijela; Krstic, Branka; Jankov, Ratko M.; Gavrović-Jankulović, Marija

(Wiley-Blackwell, Hoboken, 2012)

TY  - JOUR
AU  - Popović, Milica M.
AU  - Bulajic, Aleksandra
AU  - Ristic, Danijela
AU  - Krstic, Branka
AU  - Jankov, Ratko M.
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1545
AB  - BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. Copyright (c) 2012 Society of Chemical Industry
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
VL  - 92
IS  - 15
SP  - 3072
EP  - 3078
DO  - 10.1002/jsfa.5728
UR  - Kon_2376
ER  - 
@article{
author = "Popović, Milica M. and Bulajic, Aleksandra and Ristic, Danijela and Krstic, Branka and Jankov, Ratko M. and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "BACKGROUND: Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. Copyright (c) 2012 Society of Chemical Industry",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit",
volume = "92",
number = "15",
pages = "3072-3078",
doi = "10.1002/jsfa.5728",
url = "Kon_2376"
}
Popović, M. M., Bulajic, A., Ristic, D., Krstic, B., Jankov, R. M.,& Gavrović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture
Wiley-Blackwell, Hoboken., 92(15), 3072-3078.
https://doi.org/10.1002/jsfa.5728
Kon_2376
Popović MM, Bulajic A, Ristic D, Krstic B, Jankov RM, Gavrović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078.
doi:10.1002/jsfa.5728
Kon_2376 .
Popović, Milica M., Bulajic, Aleksandra, Ristic, Danijela, Krstic, Branka, Jankov, Ratko M., Gavrović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078,
https://doi.org/10.1002/jsfa.5728 .,
Kon_2376 .
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