TY  - THES
AU  - Stanojević, Nataša
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5459
AB  - Enzimi su biokatalizatori koji mogu specifično i efikasno katalizovati veliki broj različitih hemijskih reakcija. Zbog svojih osobina nalaze širok spektar primjene u svim granama industrije. Međutim, uslovi pod kojim je potreban rad enzima, najčešće nisu povoljni za strukturu i funkciju biokatalizatora. Proteinski inženjering omogućava dizajn proteina sa modifikacijama koje poboljšavaju osobine enzima i prilagođavaju ih ciljanoj upotrebi.
Cilj ovog rada bio je dobijanje aktivnijeg oblika peroksidaze iz rena (C1A) uz pomoć proteinskog inženjeringa. Mjesto specifičnom mutacijom moguće je uticati direktno na specifične osobine enzima u cilju njihovih poboljšanja. Peroksidaza iz rena je dobro proučen protein, sa nizom primjena u analitičkim metodama, bioremedijaciji, organskim sintezama i sl. Stoga proizvodnja aktivnijeg oblika ovog enzima ima značaj za različite oblasti primjene.
T1  - Heterologna ekspresija i prečišćavanje mutanta R302I peroksidaze iz rena izoforme C1A u kvascu Pichia pastoris KM71H
SP  - 1
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5459
ER  - 

@mastersthesis{
author = "Stanojević, Nataša",
year = "2022",
abstract = "Enzimi su biokatalizatori koji mogu specifično i efikasno katalizovati veliki broj različitih hemijskih reakcija. Zbog svojih osobina nalaze širok spektar primjene u svim granama industrije. Međutim, uslovi pod kojim je potreban rad enzima, najčešće nisu povoljni za strukturu i funkciju biokatalizatora. Proteinski inženjering omogućava dizajn proteina sa modifikacijama koje poboljšavaju osobine enzima i prilagođavaju ih ciljanoj upotrebi.
Cilj ovog rada bio je dobijanje aktivnijeg oblika peroksidaze iz rena (C1A) uz pomoć proteinskog inženjeringa. Mjesto specifičnom mutacijom moguće je uticati direktno na specifične osobine enzima u cilju njihovih poboljšanja. Peroksidaza iz rena je dobro proučen protein, sa nizom primjena u analitičkim metodama, bioremedijaciji, organskim sintezama i sl. Stoga proizvodnja aktivnijeg oblika ovog enzima ima značaj za različite oblasti primjene.",
title = "Heterologna ekspresija i prečišćavanje mutanta R302I peroksidaze iz rena izoforme C1A u kvascu Pichia pastoris KM71H",
pages = "1-45",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5459"
}

Stanojević, N.. (2022). Heterologna ekspresija i prečišćavanje mutanta R302I peroksidaze iz rena izoforme C1A u kvascu Pichia pastoris KM71H. , 1-45.
https://hdl.handle.net/21.15107/rcub_cherry_5459

Stanojević N. Heterologna ekspresija i prečišćavanje mutanta R302I peroksidaze iz rena izoforme C1A u kvascu Pichia pastoris KM71H. 2022;:1-45.
https://hdl.handle.net/21.15107/rcub_cherry_5459 .

Stanojević, Nataša, "Heterologna ekspresija i prečišćavanje mutanta R302I peroksidaze iz rena izoforme C1A u kvascu Pichia pastoris KM71H" (2022):1-45,
https://hdl.handle.net/21.15107/rcub_cherry_5459 .

TY  - THES
AU  - Stanojević, Nataša
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4722
AB  - Cilj ovog rada je kloniranje i ekspresija izoforme C1A peroksidaze iz rena u E. coli Shuffle
soju. Nakon ekspresije, bakterijska kultura je centrifugirana, ćelije lizirane i postavljene na
dijalizu naspram vode i pufera. Enzim je prečišćen afinitetnom hromatografijom sa
imobilizovanim metalom. Određen je stepen prečišćenja, koncentracija i aktivnost dobijenog
proizvoda. Nakon toga su ispitani kinetički parametri, kao i pH optimum, temperaturna
stabilnost i temperaturni optimum.
T1  - Heterologna ekspresija, prečišćavanje i karakterizacija izoforme C1A peroksidaze iz rena u Escherichia coli Shuffle soju
SP  - 6
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4722
ER  - 

@misc{
author = "Stanojević, Nataša",
year = "2021",
abstract = "Cilj ovog rada je kloniranje i ekspresija izoforme C1A peroksidaze iz rena u E. coli Shuffle
soju. Nakon ekspresije, bakterijska kultura je centrifugirana, ćelije lizirane i postavljene na
dijalizu naspram vode i pufera. Enzim je prečišćen afinitetnom hromatografijom sa
imobilizovanim metalom. Određen je stepen prečišćenja, koncentracija i aktivnost dobijenog
proizvoda. Nakon toga su ispitani kinetički parametri, kao i pH optimum, temperaturna
stabilnost i temperaturni optimum.",
title = "Heterologna ekspresija, prečišćavanje i karakterizacija izoforme C1A peroksidaze iz rena u Escherichia coli Shuffle soju",
pages = "6-45",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4722"
}

Stanojević, N.. (2021). Heterologna ekspresija, prečišćavanje i karakterizacija izoforme C1A peroksidaze iz rena u Escherichia coli Shuffle soju. , 6-45.
https://hdl.handle.net/21.15107/rcub_cherry_4722

Stanojević N. Heterologna ekspresija, prečišćavanje i karakterizacija izoforme C1A peroksidaze iz rena u Escherichia coli Shuffle soju. 2021;:6-45.
https://hdl.handle.net/21.15107/rcub_cherry_4722 .

Stanojević, Nataša, "Heterologna ekspresija, prečišćavanje i karakterizacija izoforme C1A peroksidaze iz rena u Escherichia coli Shuffle soju" (2021):6-45,
https://hdl.handle.net/21.15107/rcub_cherry_4722 .

TY  - JOUR
AU  - Miljković, Milica
AU  - Kotur-Stevuljević, Jelena
AU  - Stefanović, Aleksandra
AU  - Željković, Aleksandra
AU  - Vekić, Jelena
AU  - Gojković, Tamara
AU  - Bogavac-Stanojević, Nataša
AU  - Nikolić, Milan
AU  - Simić-Ogrizović, Sanja
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2320
AB  - Unfavorable lipid profile is a major risk factor for cardiovascular disease in renal pathology. In this study, we compared chronic renal patients and healthy controls with different LDL phenotypes (A or B) in respect of various biochemical parameters related to cardiovascular disease. Oxidative stress and anti-oxidative defense parameters [thiobarbituric acid-reacting substances (TBARS), total oxidative status (TOS), total anti-oxidative status (TAS), total protein sulfhydryl (-SH) groups], as well as red blood cell cholesterol distribution were assessed in 40 renal patients and 40 control subjects by standardized assays. LDL particle diameters were determined by polyacrylamide gradient gel electrophoresis. LDL particles are subdivided according to their size into large LDL A phenotype (diameter  gt  25.5 nm) and small LDL B phenotype (diameter aecurrency sign25.5 nm). Renal patients with LDL A phenotype had increased oxidative stress (TOS: p  lt  0.01, and TBARS: p  lt  0.001) and decreased total SH- groups (p  lt  0.001) compared to controls with the same LDL phenotype. A notable decrease in hemoglobin-cholesterol adduct was detected in patients with LDL A phenotype (p  lt  0.001) and LDL B phenotype (p  lt  0.05) compared with appropriate controls. LDL B phenotype was characterized with increased TBARS (p  lt  0.05) compared with LDL A phenotype in control group. Increased oxidative stress, decreased anti-oxidative defense followed with unfavorable changes in hemoglobin-cholesterol binding capacity, could have important influence on cardiovascular disease risk in renal patients regardless of LDL phenotype.
PB  - Springer, Dordrecht
T2  - International Urology and Nephrology
T1  - Oxidative stress and hemoglobin-cholesterol adduct in renal patients with different LDL phenotypes
VL  - 48
IS  - 10
SP  - 1683
EP  - 1690
DO  - 10.1007/s11255-016-1358-0
ER  - 

@article{
author = "Miljković, Milica and Kotur-Stevuljević, Jelena and Stefanović, Aleksandra and Željković, Aleksandra and Vekić, Jelena and Gojković, Tamara and Bogavac-Stanojević, Nataša and Nikolić, Milan and Simić-Ogrizović, Sanja and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana",
year = "2016",
abstract = "Unfavorable lipid profile is a major risk factor for cardiovascular disease in renal pathology. In this study, we compared chronic renal patients and healthy controls with different LDL phenotypes (A or B) in respect of various biochemical parameters related to cardiovascular disease. Oxidative stress and anti-oxidative defense parameters [thiobarbituric acid-reacting substances (TBARS), total oxidative status (TOS), total anti-oxidative status (TAS), total protein sulfhydryl (-SH) groups], as well as red blood cell cholesterol distribution were assessed in 40 renal patients and 40 control subjects by standardized assays. LDL particle diameters were determined by polyacrylamide gradient gel electrophoresis. LDL particles are subdivided according to their size into large LDL A phenotype (diameter  gt  25.5 nm) and small LDL B phenotype (diameter aecurrency sign25.5 nm). Renal patients with LDL A phenotype had increased oxidative stress (TOS: p  lt  0.01, and TBARS: p  lt  0.001) and decreased total SH- groups (p  lt  0.001) compared to controls with the same LDL phenotype. A notable decrease in hemoglobin-cholesterol adduct was detected in patients with LDL A phenotype (p  lt  0.001) and LDL B phenotype (p  lt  0.05) compared with appropriate controls. LDL B phenotype was characterized with increased TBARS (p  lt  0.05) compared with LDL A phenotype in control group. Increased oxidative stress, decreased anti-oxidative defense followed with unfavorable changes in hemoglobin-cholesterol binding capacity, could have important influence on cardiovascular disease risk in renal patients regardless of LDL phenotype.",
publisher = "Springer, Dordrecht",
journal = "International Urology and Nephrology",
title = "Oxidative stress and hemoglobin-cholesterol adduct in renal patients with different LDL phenotypes",
volume = "48",
number = "10",
pages = "1683-1690",
doi = "10.1007/s11255-016-1358-0"
}

Miljković, M., Kotur-Stevuljević, J., Stefanović, A., Željković, A., Vekić, J., Gojković, T., Bogavac-Stanojević, N., Nikolić, M., Simić-Ogrizović, S., Spasojević-Kalimanovska, V.,& Jelić-Ivanović, Z.. (2016). Oxidative stress and hemoglobin-cholesterol adduct in renal patients with different LDL phenotypes. in International Urology and Nephrology
Springer, Dordrecht., 48(10), 1683-1690.
https://doi.org/10.1007/s11255-016-1358-0

Miljković M, Kotur-Stevuljević J, Stefanović A, Željković A, Vekić J, Gojković T, Bogavac-Stanojević N, Nikolić M, Simić-Ogrizović S, Spasojević-Kalimanovska V, Jelić-Ivanović Z. Oxidative stress and hemoglobin-cholesterol adduct in renal patients with different LDL phenotypes. in International Urology and Nephrology. 2016;48(10):1683-1690.
doi:10.1007/s11255-016-1358-0 .

Miljković, Milica, Kotur-Stevuljević, Jelena, Stefanović, Aleksandra, Željković, Aleksandra, Vekić, Jelena, Gojković, Tamara, Bogavac-Stanojević, Nataša, Nikolić, Milan, Simić-Ogrizović, Sanja, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, "Oxidative stress and hemoglobin-cholesterol adduct in renal patients with different LDL phenotypes" in International Urology and Nephrology, 48, no. 10 (2016):1683-1690,
https://doi.org/10.1007/s11255-016-1358-0 . .

TY  - CONF
AU  - Miljković, Milica
AU  - Kotur-Stevuljević, Jelena
AU  - Stefanović, A.
AU  - Željković, Aleksandra
AU  - Vekić, Jelena
AU  - Gojković, Tamara
AU  - Bogavac-Stanojević, Nataša
AU  - Nikolić, Milan
AU  - Simić-Ogrizović, Sanja
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1954
PB  - Elsevier Ireland Ltd, Clare
C3  - Atherosclerosis
T1  - Potential Markers in the Assessment of Risk for Development of Atherosclerosis in Patients with Chronic Renal Disease
VL  - 241
IS  - 1
DO  - 10.1016/j.atherosclerosis.2015.04.912
ER  - 

@conference{
author = "Miljković, Milica and Kotur-Stevuljević, Jelena and Stefanović, A. and Željković, Aleksandra and Vekić, Jelena and Gojković, Tamara and Bogavac-Stanojević, Nataša and Nikolić, Milan and Simić-Ogrizović, Sanja and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana",
year = "2015",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Atherosclerosis",
title = "Potential Markers in the Assessment of Risk for Development of Atherosclerosis in Patients with Chronic Renal Disease",
volume = "241",
number = "1",
doi = "10.1016/j.atherosclerosis.2015.04.912"
}

Miljković, M., Kotur-Stevuljević, J., Stefanović, A., Željković, A., Vekić, J., Gojković, T., Bogavac-Stanojević, N., Nikolić, M., Simić-Ogrizović, S., Spasojević-Kalimanovska, V.,& Jelić-Ivanović, Z.. (2015). Potential Markers in the Assessment of Risk for Development of Atherosclerosis in Patients with Chronic Renal Disease. in Atherosclerosis
Elsevier Ireland Ltd, Clare., 241(1).
https://doi.org/10.1016/j.atherosclerosis.2015.04.912

Miljković M, Kotur-Stevuljević J, Stefanović A, Željković A, Vekić J, Gojković T, Bogavac-Stanojević N, Nikolić M, Simić-Ogrizović S, Spasojević-Kalimanovska V, Jelić-Ivanović Z. Potential Markers in the Assessment of Risk for Development of Atherosclerosis in Patients with Chronic Renal Disease. in Atherosclerosis. 2015;241(1).
doi:10.1016/j.atherosclerosis.2015.04.912 .

Miljković, Milica, Kotur-Stevuljević, Jelena, Stefanović, A., Željković, Aleksandra, Vekić, Jelena, Gojković, Tamara, Bogavac-Stanojević, Nataša, Nikolić, Milan, Simić-Ogrizović, Sanja, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, "Potential Markers in the Assessment of Risk for Development of Atherosclerosis in Patients with Chronic Renal Disease" in Atherosclerosis, 241, no. 1 (2015),
https://doi.org/10.1016/j.atherosclerosis.2015.04.912 . .