TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Stanojević, Sladjana P.
AU  - Pešić, Mirjana B.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6286
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
T2  - Antioxidants
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
VL  - 12
IS  - 7
SP  - 1424
DO  - 10.3390/antiox12071424
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš M. and Tešić, Živoslav Lj. and Stanojević, Sladjana P. and Pešić, Mirjana B.",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
journal = "Antioxidants, Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
volume = "12",
number = "7",
pages = "1424",
doi = "10.3390/antiox12071424"
}

Kostić, A. Ž., Milinčić, D. D., Špirović Trifunović, B., Nedić, N., Gašić, U. M., Tešić, Ž. Lj., Stanojević, S. P.,& Pešić, M. B.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants, 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić AŽ, Milinčić DD, Špirović Trifunović B, Nedić N, Gašić UM, Tešić ŽL, Stanojević SP, Pešić MB. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš M., Tešić, Živoslav Lj., Stanojević, Sladjana P., Pešić, Mirjana B., "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4622
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - MDPI
T2  - Antioxidants
T1  - Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen
VL  - 10
IS  - 7
SP  - 1091
DO  - 10.3390/antiox10071091
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen",
volume = "10",
number = "7",
pages = "1091",
doi = "10.3390/antiox10071091"
}

Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants
MDPI., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - DATA
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Nedić, Nebojša
AU  - Gašić, Uroš M.
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4623
PB  - MDPI
T2  - Antioxidants
T1  - Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4623
ER  - 

@misc{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Nedić, Nebojša and Gašić, Uroš M. and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
publisher = "MDPI",
journal = "Antioxidants",
title = "Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4623"
}

Kostić, A. Ž., Milinčić, D. D., Nedić, N., Gašić, U. M., Špirović Trifunović, B., Vojt, D., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.. in Antioxidants
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_4623

Kostić AŽ, Milinčić DD, Nedić N, Gašić UM, Špirović Trifunović B, Vojt D, Tešić ŽL, Pešić MB. Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091.. in Antioxidants. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4623 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Nedić, Nebojša, Gašić, Uroš M., Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav Lj., Pešić, Mirjana B., "Supplementary data for the article:Kostić, A. Ž.; Milinčić, D. D.; Nedić, N.; Gašić, U. M.; Špirović Trifunović, B.; Vojt, D.; Tešić, Ž. L.; Pešić, M. B. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara Scolymus) Pollen. Antioxidants 2021, 10 (7), 1091. https://doi.org/10.3390/antiox10071091." in Antioxidants (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4623 .

TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Špirović-Trifunović, Bojana 
AU  - Tešić, Živoslav Lj.
AU  - Tosti, Tomislav
AU  - Dramićanin, Aleksandra M.
AU  - Barać, Miroljub B.
AU  - Pešić, Mirjana B.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4006
AB  - The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly ω-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value
VL  - 85
IS  - 3
SP  - 305
EP  - 319
DO  - 10.2298/JSC190713117M
ER  - 

@article{
author = "Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Špirović-Trifunović, Bojana  and Tešić, Živoslav Lj. and Tosti, Tomislav and Dramićanin, Aleksandra M. and Barać, Miroljub B. and Pešić, Mirjana B.",
year = "2020",
abstract = "The aim of this study was to determine fatty acid and soluble sugar profiles of the grape seed flour originated from non-fermented dried pomace of international and autochthonous grape varieties in order to estimate their potential nutritional value. The grape seed flours were obtained from the grapes harvested in technological maturity. It has been shown that grape seed flours contained significant quantities of unsaturated fatty acids (UFAs), especially linoleic fatty acids, whose content ranged from 61.15 - 83.47 %. Oleic acid mostly contributed to the content of monounsaturated fatty acids, while the stearic acid was the most abundant saturated fatty acid (SFA). Among polyunsaturated fatty acids, mainly ω-6 FAs, were the most represented. The tested grape seed flours had the high UFA/SFA ratio (3.63-11.09), low atherogenicity (0.04-0.13) and thrombogenicity (0.16-0.47) indices. Fifteen different sugars were found in analysed samples with the total concentration ranging from 40588 to 91319 mg/kg seed with fructose and glucose as the most abundant. Principal component analysis based on the content of FAs and soluble sugars revealed unique composition of the seed flour of Prokupac variety. These findings indicate that the tested grape seed flours is a good source of nutritionally valuable FAs and sugars that can play an important role in the formulation of a new functional food products.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value",
volume = "85",
number = "3",
pages = "305-319",
doi = "10.2298/JSC190713117M"
}

Milinčić, D. D., Kostić, A. Ž., Špirović-Trifunović, B., Tešić, Ž. Lj., Tosti, T., Dramićanin, A. M., Barać, M. B.,& Pešić, M. B.. (2020). Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 85(3), 305-319.
https://doi.org/10.2298/JSC190713117M

Milinčić DD, Kostić AŽ, Špirović-Trifunović B, Tešić ŽL, Tosti T, Dramićanin AM, Barać MB, Pešić MB. Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value. in Journal of the Serbian Chemical Society. 2020;85(3):305-319.
doi:10.2298/JSC190713117M .

Milinčić, Danijel D., Kostić, Aleksandar Ž., Špirović-Trifunović, Bojana , Tešić, Živoslav Lj., Tosti, Tomislav, Dramićanin, Aleksandra M., Barać, Miroljub B., Pešić, Mirjana B., "Grape seed flour of different grape pomaces: Fatty acid profile, soluble sugar profile and nutritional value" in Journal of the Serbian Chemical Society, 85, no. 3 (2020):305-319,
https://doi.org/10.2298/JSC190713117M . .