TY  - JOUR
AU  - Stolić Jovanović, Aleksandra
AU  - Martinović, Milica
AU  - Žugić, Ana
AU  - Nešić, Ivana
AU  - Tosti, Tomislav
AU  - Blagojević, Stevan
AU  - Tadić, Vanja M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6195
AB  - The dual controlled release of emulgels makes them efficient drug delivery systems of increasing interest. The framework of this study was to incorporate selected L-ascorbic acid derivatives into emulgels. From the formulated emulgels, the release profiles of actives were evaluated considering their different polarities and concentrations, and consequently their effectiveness on the skin via a long-term in vivo study that lasted for 30 days was determined. Skin effects were assessed by measuring the electrical capacitance of the stratum corneum (EC), trans-epidermal water loss (TEWL), melanin index (MI) and skin pH. In addition, the sensory and textural properties of emulgel formulations were compared with each other. The changes in the rate of the release of the L-ascorbic acid derivatives were monitored using the Franz diffusion cells. The obtained data were statistically significant, and indicated an increase in the degree of hydration of the skin and skin whitening potential, while no significant changes in TEWL and pH values were detected. The consistency, firmness and stickiness of the emulgels were estimated by volunteers applying the established sensory evaluation protocol. In addition, it was revealed that the difference in hydrophilic/lipophilic properties of L-ascorbic acid derivatives influenced their release profiles without changing their textural characteristics. Therefore, this study highlighted emulgels as L-ascorbic acid suitable carrier systems and one of the promising candidates as novel drug delivery systems.
PB  - MDPI
T2  - Pharmaceutics
T1  - Derivatives of L-Ascorbic Acid in Emulgel: Development and Comprehensive Evaluation of the Topical Delivery System
VL  - 15
IS  - 3
SP  - 813
DO  - 10.3390/pharmaceutics15030813
ER  - 

@article{
author = "Stolić Jovanović, Aleksandra and Martinović, Milica and Žugić, Ana and Nešić, Ivana and Tosti, Tomislav and Blagojević, Stevan and Tadić, Vanja M.",
year = "2023",
abstract = "The dual controlled release of emulgels makes them efficient drug delivery systems of increasing interest. The framework of this study was to incorporate selected L-ascorbic acid derivatives into emulgels. From the formulated emulgels, the release profiles of actives were evaluated considering their different polarities and concentrations, and consequently their effectiveness on the skin via a long-term in vivo study that lasted for 30 days was determined. Skin effects were assessed by measuring the electrical capacitance of the stratum corneum (EC), trans-epidermal water loss (TEWL), melanin index (MI) and skin pH. In addition, the sensory and textural properties of emulgel formulations were compared with each other. The changes in the rate of the release of the L-ascorbic acid derivatives were monitored using the Franz diffusion cells. The obtained data were statistically significant, and indicated an increase in the degree of hydration of the skin and skin whitening potential, while no significant changes in TEWL and pH values were detected. The consistency, firmness and stickiness of the emulgels were estimated by volunteers applying the established sensory evaluation protocol. In addition, it was revealed that the difference in hydrophilic/lipophilic properties of L-ascorbic acid derivatives influenced their release profiles without changing their textural characteristics. Therefore, this study highlighted emulgels as L-ascorbic acid suitable carrier systems and one of the promising candidates as novel drug delivery systems.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Derivatives of L-Ascorbic Acid in Emulgel: Development and Comprehensive Evaluation of the Topical Delivery System",
volume = "15",
number = "3",
pages = "813",
doi = "10.3390/pharmaceutics15030813"
}

Stolić Jovanović, A., Martinović, M., Žugić, A., Nešić, I., Tosti, T., Blagojević, S.,& Tadić, V. M.. (2023). Derivatives of L-Ascorbic Acid in Emulgel: Development and Comprehensive Evaluation of the Topical Delivery System. in Pharmaceutics
MDPI., 15(3), 813.
https://doi.org/10.3390/pharmaceutics15030813

Stolić Jovanović A, Martinović M, Žugić A, Nešić I, Tosti T, Blagojević S, Tadić VM. Derivatives of L-Ascorbic Acid in Emulgel: Development and Comprehensive Evaluation of the Topical Delivery System. in Pharmaceutics. 2023;15(3):813.
doi:10.3390/pharmaceutics15030813 .

Stolić Jovanović, Aleksandra, Martinović, Milica, Žugić, Ana, Nešić, Ivana, Tosti, Tomislav, Blagojević, Stevan, Tadić, Vanja M., "Derivatives of L-Ascorbic Acid in Emulgel: Development and Comprehensive Evaluation of the Topical Delivery System" in Pharmaceutics, 15, no. 3 (2023):813,
https://doi.org/10.3390/pharmaceutics15030813 . .

TY  - JOUR
AU  - Paunović, Verica
AU  - Kosic, Milica
AU  - Đorđević, S.
AU  - Žugić, Ana
AU  - Đalinac, Nataša
AU  - Gašić, Uroš M.
AU  - Trajković, Vladimir S.
AU  - Harhaji-Trajkovic, Ljubica
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2434
AB  - Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27. MVE induced oxidative stress, while antioxidants abrogated its antitumor effect. Furthermore, MVE induced mitochondrial depolarization, activation of caspase-9 and -3, Parp cleavage, phosphatidylserine exposure and DNA fragmentation. The mitochondrial apoptotic pathway was associated with the up-regulation of proapoptotic genes Pten, Bak1, Apaf1, and Puma and down-regulation of antiapoptotic genes survivin and Xiap. MVE also stimulated the expression of autophagy-related genes Atg5, Atg7, Atg12, Beclin-1, Gabarab and Sqstm1, as well as LC3-I conversion to the autophagosome associated LC3-II, while autophagy inhibitors exacerbated its cytotoxicity. Finally, the most abundant phenolic components of MVE, ferulic, p-hydroxybenzoic, caffeic and chlorogenic acids, did not exert a profound effect on viability of tumor cells, suggesting that other components individually or in concert are the mediators of the extracts' cytotoxicity. By demonstrating the ability of MVE to inhibit proliferation, induce apoptosis and cytoprotective autophagy, our results suggest that MVE, alone or combined with autophagy inhibitors, could be a good candidate for anti-melanoma and anti-glioma therapy.
PB  - C M B  Assoc, Poitiers
T2  - Cellular and Molecular Biology
T1  - Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro
VL  - 62
IS  - 11
SP  - 108
EP  - 114
DO  - 10.14715/cmb/2016.62.11.18
ER  - 

@article{
author = "Paunović, Verica and Kosic, Milica and Đorđević, S. and Žugić, Ana and Đalinac, Nataša and Gašić, Uroš M. and Trajković, Vladimir S. and Harhaji-Trajkovic, Ljubica",
year = "2016",
abstract = "Marrubium vulgare is a European medicinal plant with numerous beneficial effects on human health. The aim of the study was to isolate the plant ethanolic extract (MVE) and to investigate its anti-melanoma and anti-glioma effects. MVE was prepared by the modified pharmacopoeial percolation method and characterized by UHPLC-LTQ OrbiTrap MS. MVE dose-dependently reduced viability of melanoma (B16) and glioma (U251) cells, but not peripheral blood mononuclear cells. It arrested cell cycle in S+G2/M phase, which was associated with the activation of MAP kinase p38 and up-regulation of antiproliferative genes p53, p21 and p27. MVE induced oxidative stress, while antioxidants abrogated its antitumor effect. Furthermore, MVE induced mitochondrial depolarization, activation of caspase-9 and -3, Parp cleavage, phosphatidylserine exposure and DNA fragmentation. The mitochondrial apoptotic pathway was associated with the up-regulation of proapoptotic genes Pten, Bak1, Apaf1, and Puma and down-regulation of antiapoptotic genes survivin and Xiap. MVE also stimulated the expression of autophagy-related genes Atg5, Atg7, Atg12, Beclin-1, Gabarab and Sqstm1, as well as LC3-I conversion to the autophagosome associated LC3-II, while autophagy inhibitors exacerbated its cytotoxicity. Finally, the most abundant phenolic components of MVE, ferulic, p-hydroxybenzoic, caffeic and chlorogenic acids, did not exert a profound effect on viability of tumor cells, suggesting that other components individually or in concert are the mediators of the extracts' cytotoxicity. By demonstrating the ability of MVE to inhibit proliferation, induce apoptosis and cytoprotective autophagy, our results suggest that MVE, alone or combined with autophagy inhibitors, could be a good candidate for anti-melanoma and anti-glioma therapy.",
publisher = "C M B  Assoc, Poitiers",
journal = "Cellular and Molecular Biology",
title = "Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro",
volume = "62",
number = "11",
pages = "108-114",
doi = "10.14715/cmb/2016.62.11.18"
}

Paunović, V., Kosic, M., Đorđević, S., Žugić, A., Đalinac, N., Gašić, U. M., Trajković, V. S.,& Harhaji-Trajkovic, L.. (2016). Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro. in Cellular and Molecular Biology
C M B  Assoc, Poitiers., 62(11), 108-114.
https://doi.org/10.14715/cmb/2016.62.11.18

Paunović V, Kosic M, Đorđević S, Žugić A, Đalinac N, Gašić UM, Trajković VS, Harhaji-Trajkovic L. Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro. in Cellular and Molecular Biology. 2016;62(11):108-114.
doi:10.14715/cmb/2016.62.11.18 .

Paunović, Verica, Kosic, Milica, Đorđević, S., Žugić, Ana, Đalinac, Nataša, Gašić, Uroš M., Trajković, Vladimir S., Harhaji-Trajkovic, Ljubica, "Marrubium vulgare ethanolic extract induces proliferation block, apoptosis, and cytoprotective autophagy in cancer cells in vitro" in Cellular and Molecular Biology, 62, no. 11 (2016):108-114,
https://doi.org/10.14715/cmb/2016.62.11.18 . .