TY  - JOUR
AU  - Zelenović, Nevena D.
AU  - Filipović, Lidija
AU  - Popović, Milica M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6356
AB  - Biotechnological and biomedical applications of antibodies have been on a steady rise since the 1980s. As unique and highly specific bioreagents, monoclonal antibodies (mAbs) have been widely exploited and approved as therapeutic agents. However, the use of mAbs has limitations for therapeutic applications. Antibody fragments (AbFs) with preserved antigen-binding sites have a significant potential to overcome the disadvantages of conventional mAbs, such as heterogeneous tissue distribution after systemic administration, especially in solid tumors, and Fc-mediated bystander activation of the immune system. AbFs possess better biodistribution coefficient due to lower molecular weight. They preserve the functional features of mAbs, such as antigen specificity and binding, while at the same time, ensuring much better tissue penetration. An additional benefit of AbFs is the possibility of their production in bacterial and yeast cells due to the small size, more robust structure, and lack of posttranslational modifications. In this review, we described current approaches to the AbF production with recent examples of AbF synthesis in bacterial and yeast expression systems and methods for the production optimization.
PB  - Springer
T2  - Biochemistry (Moscow)
T1  - Recent Developments in Bioprocessing of Recombinant Antibody Fragments
VL  - 88
IS  - 9
SP  - 1191
EP  - 1204
DO  - 10.1134/S0006297923090018
ER  - 

@article{
author = "Zelenović, Nevena D. and Filipović, Lidija and Popović, Milica M.",
year = "2023",
abstract = "Biotechnological and biomedical applications of antibodies have been on a steady rise since the 1980s. As unique and highly specific bioreagents, monoclonal antibodies (mAbs) have been widely exploited and approved as therapeutic agents. However, the use of mAbs has limitations for therapeutic applications. Antibody fragments (AbFs) with preserved antigen-binding sites have a significant potential to overcome the disadvantages of conventional mAbs, such as heterogeneous tissue distribution after systemic administration, especially in solid tumors, and Fc-mediated bystander activation of the immune system. AbFs possess better biodistribution coefficient due to lower molecular weight. They preserve the functional features of mAbs, such as antigen specificity and binding, while at the same time, ensuring much better tissue penetration. An additional benefit of AbFs is the possibility of their production in bacterial and yeast cells due to the small size, more robust structure, and lack of posttranslational modifications. In this review, we described current approaches to the AbF production with recent examples of AbF synthesis in bacterial and yeast expression systems and methods for the production optimization.",
publisher = "Springer",
journal = "Biochemistry (Moscow)",
title = "Recent Developments in Bioprocessing of Recombinant Antibody Fragments",
volume = "88",
number = "9",
pages = "1191-1204",
doi = "10.1134/S0006297923090018"
}

Zelenović, N. D., Filipović, L.,& Popović, M. M.. (2023). Recent Developments in Bioprocessing of Recombinant Antibody Fragments. in Biochemistry (Moscow)
Springer., 88(9), 1191-1204.
https://doi.org/10.1134/S0006297923090018

Zelenović ND, Filipović L, Popović MM. Recent Developments in Bioprocessing of Recombinant Antibody Fragments. in Biochemistry (Moscow). 2023;88(9):1191-1204.
doi:10.1134/S0006297923090018 .

Zelenović, Nevena D., Filipović, Lidija, Popović, Milica M., "Recent Developments in Bioprocessing of Recombinant Antibody Fragments" in Biochemistry (Moscow), 88, no. 9 (2023):1191-1204,
https://doi.org/10.1134/S0006297923090018 . .

TY  - JOUR
AU  - Zelenović, Nevena D.
AU  - Kojadinović, Milica I.
AU  - Filipović, Lidija
AU  - Vučić, Vesna M.
AU  - Milčić, Miloš K.
AU  - Arsic, Aleksndra
AU  - Popović, Milica M.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6257
AB  - Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.
PB  - SAGE Publications
T2  - Natural Product Communications
T1  - Interactions of Different Urolithins With Bovine Serum Albumin
VL  - 18
IS  - 5
DO  - 10.1177/1934578X231169366
ER  - 

@article{
author = "Zelenović, Nevena D. and Kojadinović, Milica I. and Filipović, Lidija and Vučić, Vesna M. and Milčić, Miloš K. and Arsic, Aleksndra and Popović, Milica M.",
year = "2023",
abstract = "Backgound/ObjectivesUrolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest.MethodsA powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein.ResultsThe spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236   ±   5706 and 69653   ±   14922, respectively, while for UROAG and UROBG, these values were 15179   ±   2770 and 9462   ±   1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site.ConclusionThe aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.",
publisher = "SAGE Publications",
journal = "Natural Product Communications",
title = "Interactions of Different Urolithins With Bovine Serum Albumin",
volume = "18",
number = "5",
doi = "10.1177/1934578X231169366"
}

Zelenović, N. D., Kojadinović, M. I., Filipović, L., Vučić, V. M., Milčić, M. K., Arsic, A.,& Popović, M. M.. (2023). Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications
SAGE Publications., 18(5).
https://doi.org/10.1177/1934578X231169366

Zelenović ND, Kojadinović MI, Filipović L, Vučić VM, Milčić MK, Arsic A, Popović MM. Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications. 2023;18(5).
doi:10.1177/1934578X231169366 .

Zelenović, Nevena D., Kojadinović, Milica I., Filipović, Lidija, Vučić, Vesna M., Milčić, Miloš K., Arsic, Aleksndra, Popović, Milica M., "Interactions of Different Urolithins With Bovine Serum Albumin" in Natural Product Communications, 18, no. 5 (2023),
https://doi.org/10.1177/1934578X231169366 . .

TY  - JOUR
AU  - Filipović, Lidija
AU  - Spasojević, Milica
AU  - Prodanović, Radivoje
AU  - Korać, Aleksandra
AU  - Matijaševic, Suzana
AU  - Brajušković, Goran
AU  - de Marco, Ario
AU  - Popović, Milica M.
PY  - 2022
UR  - https://pubmed.ncbi.nlm.nih.gov/35301156/
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5152
AB  - Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.
PB  - Elsevier
T2  - New Biotechnology
T1  - Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer
VL  - 69
SP  - 36
EP  - 48
DO  - 10.1016/j.nbt.2022.03.001
ER  - 

@article{
author = "Filipović, Lidija and Spasojević, Milica and Prodanović, Radivoje and Korać, Aleksandra and Matijaševic, Suzana and Brajušković, Goran and de Marco, Ario and Popović, Milica M.",
year = "2022",
abstract = "Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer",
volume = "69",
pages = "36-48",
doi = "10.1016/j.nbt.2022.03.001"
}

Filipović, L., Spasojević, M., Prodanović, R., Korać, A., Matijaševic, S., Brajušković, G., de Marco, A.,& Popović, M. M.. (2022). Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer. in New Biotechnology
Elsevier., 69, 36-48.
https://doi.org/10.1016/j.nbt.2022.03.001

Filipović L, Spasojević M, Prodanović R, Korać A, Matijaševic S, Brajušković G, de Marco A, Popović MM. Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer. in New Biotechnology. 2022;69:36-48.
doi:10.1016/j.nbt.2022.03.001 .

Filipović, Lidija, Spasojević, Milica, Prodanović, Radivoje, Korać, Aleksandra, Matijaševic, Suzana, Brajušković, Goran, de Marco, Ario, Popović, Milica M., "Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer" in New Biotechnology, 69 (2022):36-48,
https://doi.org/10.1016/j.nbt.2022.03.001 . .

TY  - DATA
AU  - Filipović, Lidija
AU  - Spasojević, Milica
AU  - Prodanović, Radivoje
AU  - Korać, Aleksandra
AU  - Matijaševic, Suzana
AU  - Brajušković, Goran
AU  - de Marco, Ario
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5152
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5153
AB  - Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.
PB  - Elsevier
T2  - New Biotechnology
T1  - Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5153
ER  - 

@misc{
author = "Filipović, Lidija and Spasojević, Milica and Prodanović, Radivoje and Korać, Aleksandra and Matijaševic, Suzana and Brajušković, Goran and de Marco, Ario and Popović, Milica M.",
year = "2022",
abstract = "Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5153"
}

Filipović, L., Spasojević, M., Prodanović, R., Korać, A., Matijaševic, S., Brajušković, G., de Marco, A.,& Popović, M. M.. (2022). Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.. in New Biotechnology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_5153

Filipović L, Spasojević M, Prodanović R, Korać A, Matijaševic S, Brajušković G, de Marco A, Popović MM. Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.. in New Biotechnology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5153 .

Filipović, Lidija, Spasojević, Milica, Prodanović, Radivoje, Korać, Aleksandra, Matijaševic, Suzana, Brajušković, Goran, de Marco, Ario, Popović, Milica M., "Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001." in New Biotechnology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5153 .

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4989
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 

@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}

FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057

FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .

FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4997
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 

@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}

FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057

FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .

FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

TY  - JOUR
AU  - Matijašević Joković, Suzana
AU  - Dobrijević, Zorana
AU  - Kotarac, Nevena
AU  - Filipović, Lidija
AU  - Popović, Milica M.
AU  - Korać, Aleksandra
AU  - Vuković, Ivan
AU  - Savić-Pavićević, Dušanka
AU  - Brajušković, Goran
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5782
AB  - MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in
multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs
in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify
their significance as PCa biomarkers, as well as to compare different types of specimens as a source
of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with
PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained
from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and
miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression
of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of
exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients
with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we
did not find an association with the values of standard prognostic parameters of PCa, nor with cancer
aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21
expression levels in PCa.
PB  - MDPI
T2  - Genes
T1  - MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
VL  - 13
IS  - 12
SP  - 2320
DO  - 10.3390/genes13122320
ER  - 

@article{
author = "Matijašević Joković, Suzana and Dobrijević, Zorana and Kotarac, Nevena and Filipović, Lidija and Popović, Milica M. and Korać, Aleksandra and Vuković, Ivan and Savić-Pavićević, Dušanka and Brajušković, Goran",
year = "2022",
abstract = "MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in
multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs
in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify
their significance as PCa biomarkers, as well as to compare different types of specimens as a source
of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with
PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained
from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and
miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression
of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of
exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients
with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we
did not find an association with the values of standard prognostic parameters of PCa, nor with cancer
aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21
expression levels in PCa.",
publisher = "MDPI",
journal = "Genes",
title = "MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes",
volume = "13",
number = "12",
pages = "2320",
doi = "10.3390/genes13122320"
}

Matijašević Joković, S., Dobrijević, Z., Kotarac, N., Filipović, L., Popović, M. M., Korać, A., Vuković, I., Savić-Pavićević, D.,& Brajušković, G.. (2022). MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes. in Genes
MDPI., 13(12), 2320.
https://doi.org/10.3390/genes13122320

Matijašević Joković S, Dobrijević Z, Kotarac N, Filipović L, Popović MM, Korać A, Vuković I, Savić-Pavićević D, Brajušković G. MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes. in Genes. 2022;13(12):2320.
doi:10.3390/genes13122320 .

Matijašević Joković, Suzana, Dobrijević, Zorana, Kotarac, Nevena, Filipović, Lidija, Popović, Milica M., Korać, Aleksandra, Vuković, Ivan, Savić-Pavićević, Dušanka, Brajušković, Goran, "MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes" in Genes, 13, no. 12 (2022):2320,
https://doi.org/10.3390/genes13122320 . .

TY  - CONF
AU  - Balaž, Ana Marija
AU  - Crnoglavac Popović, Milica
AU  - Stanišić, Marija D.
AU  - Ristić, Predrag
AU  - Senćanski, Milan
AU  - Todorović, Tamara
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5747
AB  - Enzyme immobilization enables maintenance of enzyme activity and structural stability even in adverse conditions 1. Structural changes in enzymes that can occur due to the action of organic solvents, inhibitors or increased temperature can be prevented by immobilization of the enzymes in metal–organic frameworks (MOFs). It is reported that several enzymes, such as cytochrome c and horseradish peroxidase (HRP) have been successfully incorporated into MOFs 2. The aim of this work is to produce wild type horseradish peroxidase, isoform C1A, and several mutants specially designed to increase the activity and stability of HRP while immobilized within selected MOFs. Wild type and its variants were produced in metalotrophic yeast, Pichia pastoris KM71H strain, their activity and basic kinetic parameters were determined and compared prior imobilization.
C3  - Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac
T1  - Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain
SP  - 49
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5747
ER  - 

@conference{
author = "Balaž, Ana Marija and Crnoglavac Popović, Milica and Stanišić, Marija D. and Ristić, Predrag and Senćanski, Milan and Todorović, Tamara and Prodanović, Radivoje",
year = "2021",
abstract = "Enzyme immobilization enables maintenance of enzyme activity and structural stability even in adverse conditions 1. Structural changes in enzymes that can occur due to the action of organic solvents, inhibitors or increased temperature can be prevented by immobilization of the enzymes in metal–organic frameworks (MOFs). It is reported that several enzymes, such as cytochrome c and horseradish peroxidase (HRP) have been successfully incorporated into MOFs 2. The aim of this work is to produce wild type horseradish peroxidase, isoform C1A, and several mutants specially designed to increase the activity and stability of HRP while immobilized within selected MOFs. Wild type and its variants were produced in metalotrophic yeast, Pichia pastoris KM71H strain, their activity and basic kinetic parameters were determined and compared prior imobilization.",
journal = "Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac",
title = "Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain",
pages = "49-49",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5747"
}

Balaž, A. M., Crnoglavac Popović, M., Stanišić, M. D., Ristić, P., Senćanski, M., Todorović, T.,& Prodanović, R.. (2021). Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac, 49-49.
https://hdl.handle.net/21.15107/rcub_cherry_5747

Balaž AM, Crnoglavac Popović M, Stanišić MD, Ristić P, Senćanski M, Todorović T, Prodanović R. Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain. in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac. 2021;:49-49.
https://hdl.handle.net/21.15107/rcub_cherry_5747 .

Balaž, Ana Marija, Crnoglavac Popović, Milica, Stanišić, Marija D., Ristić, Predrag, Senćanski, Milan, Todorović, Tamara, Prodanović, Radivoje, "Horseradish peroxidase C1A wild type gene and its variants expressed in Pichia pastoris KM71H strain" in Tenth Conference of Serbian Biochemical Society, 24 September 2021, Kragujevac (2021):49-49,
https://hdl.handle.net/21.15107/rcub_cherry_5747 .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4549
AB  - Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PB  - Springer Nature
T2  - Molecular Biology Reports
T1  - A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing
VL  - 48
IS  - 5
SP  - 4065
EP  - 4072
DO  - 10.1007/s11033-021-06416-8
ER  - 

@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
publisher = "Springer Nature",
journal = "Molecular Biology Reports",
title = "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing",
volume = "48",
number = "5",
pages = "4065-4072",
doi = "10.1007/s11033-021-06416-8"
}

Nešić, A. N., Čavić, M., Popović, M. M.,& Gavrović-Jankulović, M.. (2021). A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports
Springer Nature., 48(5), 4065-4072.
https://doi.org/10.1007/s11033-021-06416-8

Nešić AN, Čavić M, Popović MM, Gavrović-Jankulović M. A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports. 2021;48(5):4065-4072.
doi:10.1007/s11033-021-06416-8 .

Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Gavrović-Jankulović, Marija, "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing" in Molecular Biology Reports, 48, no. 5 (2021):4065-4072,
https://doi.org/10.1007/s11033-021-06416-8 . .

TY  - JOUR
AU  - Kojadinović, Milica I.
AU  - Glibetić, Marija D.
AU  - Vučić, Vesna M.
AU  - Popović, Milica M.
AU  - Vidović, Nevena Đ.
AU  - Debeljak-Martačić, Jasmina D.
AU  - Arsić, Aleksandra Č.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4791
AB  - Pomegranate juice (PJ) has potential positive effects in patients with metabolic disturbances due to a high content of polyphenols. The objective of this study was to evaluate effects of a 2-week consumption of dietary doses of PJ on blood pressure, lipid metabolism, and oxidative stress markers in overweight patients with dyslipidemia. Twenty-four patients, 8 males and 16 females, 40-60 years of age, with established overweight and dyslipidemia were randomly assigned into intervention group, who consumed 300 mL of PJ daily for 2 weeks, or control group. After 2 weeks of juice intake, intervention group had significantly lower diastolic blood pressure, low-density lipoprotein cholesterol, aminotransferase, and activity of glutathione peroxidase. Furthermore, patients who consumed PJ had reduced percentage of docosahexaenoic acid (22:6n-3, DHA) in plasma phospholipids and increased estimated activity of stearoyl-CoA desaturase. In erythrocytes, we found a significant increase in the levels of dihomo-gamma- linolenic acid (20:3n-6, DGLA) and DHA, as well as in estimated activity of Delta 6 desaturase, and a decrease in estimated activity of Delta 5 desaturase. These results show that even a short-term consumption of dietary doses of PJ exerts beneficial effects and affects lipid metabolism in overweight patients with dyslipidemia.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia
VL  - 24
IS  - 9
DO  - 10.1089/jmf.2020.0122
ER  - 

@article{
author = "Kojadinović, Milica I. and Glibetić, Marija D. and Vučić, Vesna M. and Popović, Milica M. and Vidović, Nevena Đ. and Debeljak-Martačić, Jasmina D. and Arsić, Aleksandra Č.",
year = "2021",
abstract = "Pomegranate juice (PJ) has potential positive effects in patients with metabolic disturbances due to a high content of polyphenols. The objective of this study was to evaluate effects of a 2-week consumption of dietary doses of PJ on blood pressure, lipid metabolism, and oxidative stress markers in overweight patients with dyslipidemia. Twenty-four patients, 8 males and 16 females, 40-60 years of age, with established overweight and dyslipidemia were randomly assigned into intervention group, who consumed 300 mL of PJ daily for 2 weeks, or control group. After 2 weeks of juice intake, intervention group had significantly lower diastolic blood pressure, low-density lipoprotein cholesterol, aminotransferase, and activity of glutathione peroxidase. Furthermore, patients who consumed PJ had reduced percentage of docosahexaenoic acid (22:6n-3, DHA) in plasma phospholipids and increased estimated activity of stearoyl-CoA desaturase. In erythrocytes, we found a significant increase in the levels of dihomo-gamma- linolenic acid (20:3n-6, DGLA) and DHA, as well as in estimated activity of Delta 6 desaturase, and a decrease in estimated activity of Delta 5 desaturase. These results show that even a short-term consumption of dietary doses of PJ exerts beneficial effects and affects lipid metabolism in overweight patients with dyslipidemia.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia",
volume = "24",
number = "9",
doi = "10.1089/jmf.2020.0122"
}

Kojadinović, M. I., Glibetić, M. D., Vučić, V. M., Popović, M. M., Vidović, N. Đ., Debeljak-Martačić, J. D.,& Arsić, A. Č.. (2021). Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 24(9).
https://doi.org/10.1089/jmf.2020.0122

Kojadinović MI, Glibetić MD, Vučić VM, Popović MM, Vidović NĐ, Debeljak-Martačić JD, Arsić AČ. Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia. in Journal of Medicinal Food. 2021;24(9).
doi:10.1089/jmf.2020.0122 .

Kojadinović, Milica I., Glibetić, Marija D., Vučić, Vesna M., Popović, Milica M., Vidović, Nevena Đ., Debeljak-Martačić, Jasmina D., Arsić, Aleksandra Č., "Short-Term Consumption of Pomegranate Juice Alleviates Some Metabolic Disturbances in Overweight Patients with Dyslipidemia" in Journal of Medicinal Food, 24, no. 9 (2021),
https://doi.org/10.1089/jmf.2020.0122 . .

TY  - JOUR
AU  - Tadić, D.
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
AU  - Đurić, Vojislav
AU  - Tomić-Špirić, Vesna
AU  - Rašković, Sanvila S.
AU  - Perić Popadić, Aleksandra
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3728
AB  - Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.
PB  - Elsevier
T2  - Revue Francaise d'Allergologie
T1  - A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population
VL  - 59
IS  - 7
SP  - 474
EP  - 480
DO  - 10.1016/j.reval.2019.05.006
ER  - 

@article{
author = "Tadić, D. and Popović, Milica M. and Gavrović-Jankulović, Marija and Đurić, Vojislav and Tomić-Špirić, Vesna and Rašković, Sanvila S. and Perić Popadić, Aleksandra",
year = "2019",
abstract = "Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.",
publisher = "Elsevier",
journal = "Revue Francaise d'Allergologie",
title = "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population",
volume = "59",
number = "7",
pages = "474-480",
doi = "10.1016/j.reval.2019.05.006"
}

Tadić, D., Popović, M. M., Gavrović-Jankulović, M., Đurić, V., Tomić-Špirić, V., Rašković, S. S.,& Perić Popadić, A.. (2019). A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population. in Revue Francaise d'Allergologie
Elsevier., 59(7), 474-480.
https://doi.org/10.1016/j.reval.2019.05.006

Tadić D, Popović MM, Gavrović-Jankulović M, Đurić V, Tomić-Špirić V, Rašković SS, Perić Popadić A. A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population. in Revue Francaise d'Allergologie. 2019;59(7):474-480.
doi:10.1016/j.reval.2019.05.006 .

Tadić, D., Popović, Milica M., Gavrović-Jankulović, Marija, Đurić, Vojislav, Tomić-Špirić, Vesna, Rašković, Sanvila S., Perić Popadić, Aleksandra, "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population" in Revue Francaise d'Allergologie, 59, no. 7 (2019):474-480,
https://doi.org/10.1016/j.reval.2019.05.006 . .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Zlatanova, Milena
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3799
AB  - Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.
PB  - MDPI
T2  - Biomolecules
T1  - The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines
VL  - 9
IS  - 12
SP  - 816
DO  - 10.3390/biom9120816
ER  - 

@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Zlatanova, Milena and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines",
volume = "9",
number = "12",
pages = "816",
doi = "10.3390/biom9120816"
}

Nešić, A. N., Čavić, M., Popović, M. M., Zlatanova, M., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules
MDPI., 9(12), 816.
https://doi.org/10.3390/biom9120816

Nešić AN, Čavić M, Popović MM, Zlatanova M, Pieters R, Smit J, Gavrović-Jankulović M. The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules. 2019;9(12):816.
doi:10.3390/biom9120816 .

Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Zlatanova, Milena, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines" in Biomolecules, 9, no. 12 (2019):816,
https://doi.org/10.3390/biom9120816 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Mazzega, Elisa
AU  - Toffoletto, Barbara
AU  - de Marco, Ario
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2069
AB  - Background: The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and immune-capture can represent an effective method to improve EV purification and successive analysis. Methods: Cell culture supernatant was used as a model sample for assessing the capacity of anion-exchange chromatography to separate distinct EV fractions and to isolate nanobodies by direct panning on whole EVs to recover binders specific for the native conformation of EV-surface epitopes and suitable to develop EV immune-capture reagents. Results: Anion-exchange chromatography of cell culture supernatant separated distinct protein-containing fractions and all of them were positive for CD9, a biomarker associated to some EVs. This suggested the existence of several EV fractions but did not help in separating EVs from other contaminants. We further isolated several nanobodies instrumental for implementing immune-affinity protocols. These were able to immobilize EVs from both cell culture supernatant and biological samples, to be used in ELISA, flow-cytometry, and immune-purification. Conclusions: Here we report the first successful isolation of anti-EV nanobodies for the use in immunoaffinity-based EV capture by panning a phage library directly on partially purified EVs. This achievement paves the way for the application of direct EV panning for the discovery of novel antibody-vesicle surface biomarker pairs and represents the preliminary requirement for the development of selective immune-capture that, in combination with anion-exchange chromatography, can simplify the systematic stratification of EV sub-populations and their individual characterization.
PB  - Biomed Central Ltd, London
T2  - Microbial Cell Factories
T1  - Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions
VL  - 17
DO  - 10.1186/s12934-017-0856-9
ER  - 

@article{
author = "Popović, Milica M. and Mazzega, Elisa and Toffoletto, Barbara and de Marco, Ario",
year = "2018",
abstract = "Background: The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and immune-capture can represent an effective method to improve EV purification and successive analysis. Methods: Cell culture supernatant was used as a model sample for assessing the capacity of anion-exchange chromatography to separate distinct EV fractions and to isolate nanobodies by direct panning on whole EVs to recover binders specific for the native conformation of EV-surface epitopes and suitable to develop EV immune-capture reagents. Results: Anion-exchange chromatography of cell culture supernatant separated distinct protein-containing fractions and all of them were positive for CD9, a biomarker associated to some EVs. This suggested the existence of several EV fractions but did not help in separating EVs from other contaminants. We further isolated several nanobodies instrumental for implementing immune-affinity protocols. These were able to immobilize EVs from both cell culture supernatant and biological samples, to be used in ELISA, flow-cytometry, and immune-purification. Conclusions: Here we report the first successful isolation of anti-EV nanobodies for the use in immunoaffinity-based EV capture by panning a phage library directly on partially purified EVs. This achievement paves the way for the application of direct EV panning for the discovery of novel antibody-vesicle surface biomarker pairs and represents the preliminary requirement for the development of selective immune-capture that, in combination with anion-exchange chromatography, can simplify the systematic stratification of EV sub-populations and their individual characterization.",
publisher = "Biomed Central Ltd, London",
journal = "Microbial Cell Factories",
title = "Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions",
volume = "17",
doi = "10.1186/s12934-017-0856-9"
}

Popović, M. M., Mazzega, E., Toffoletto, B.,& de Marco, A.. (2018). Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions. in Microbial Cell Factories
Biomed Central Ltd, London., 17.
https://doi.org/10.1186/s12934-017-0856-9

Popović MM, Mazzega E, Toffoletto B, de Marco A. Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions. in Microbial Cell Factories. 2018;17.
doi:10.1186/s12934-017-0856-9 .

Popović, Milica M., Mazzega, Elisa, Toffoletto, Barbara, de Marco, Ario, "Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions" in Microbial Cell Factories, 17 (2018),
https://doi.org/10.1186/s12934-017-0856-9 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - de Marco, Ario
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2138
AB  - Extracellular vesicles (EVs) play a pivotal role in cell to cell signalling in both physiological and pathological conditions. Based on their biogenesis, three main classes of EVs are recognized: exosomes, microvesicles and apoptotic bodies. Exosomes are cell-derived vesicles (EVs) present in many body fluids (blood, urine, milk, cerebrospinal fluid) ranging in size from 30 to 150 nm. Due to their involvement in numerous physiological and pathological events, cell derived exosomes in bodily fluids represent a unique source of clinically relevant and non-invasive biomarkers. Since biomolecule content present in exosomes reflects the state of the parent cell, exosome analysis and characterization may provide valuable information about the presence of aberrant processes in the cells from which they originated. Because of the large and heterogeneous scientific community working with exosomes, several purification strategies have been applied so far, which yield EV fractions largely differing for quantity and quality. Most of the present exosome isolation approaches based on ultracentrifugation (UC), ultrafiltration (UF) or precipitation, are inefficient and hard to standardize, thereby creating low reproducibility in sample quality and potentially misleading results because highly sensitivity downstream analytical techniques, such as mass spectrometry, can detect even minute traces of co-isolated contaminants. Furthermore, loss of certain exosomal fractions during purification process or damage of exosomal membrane integrity can also alter final protein and RNA profiles. As a consequence, there is a strong interest in consensus principles for the exosome purification and the search for reliable methods for selective isolation of EV sub-populations. In the present manuscript, we critically overview the most commonly used techniques used for exosome preparation such as ultracentrifugation, size-based isolation methods, precipitation and immunoaffinity (IA) and their respective applicability for purification of exosomes from clinically relevant samples.
PB  - Ame Publ Co, Shatin
T2  - TRANSLATIONAL CANCER RESEARCH
T1  - Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy
VL  - 7
DO  - 10.21037/tcr.2017.08.44
ER  - 

@article{
author = "Popović, Milica M. and de Marco, Ario",
year = "2018",
abstract = "Extracellular vesicles (EVs) play a pivotal role in cell to cell signalling in both physiological and pathological conditions. Based on their biogenesis, three main classes of EVs are recognized: exosomes, microvesicles and apoptotic bodies. Exosomes are cell-derived vesicles (EVs) present in many body fluids (blood, urine, milk, cerebrospinal fluid) ranging in size from 30 to 150 nm. Due to their involvement in numerous physiological and pathological events, cell derived exosomes in bodily fluids represent a unique source of clinically relevant and non-invasive biomarkers. Since biomolecule content present in exosomes reflects the state of the parent cell, exosome analysis and characterization may provide valuable information about the presence of aberrant processes in the cells from which they originated. Because of the large and heterogeneous scientific community working with exosomes, several purification strategies have been applied so far, which yield EV fractions largely differing for quantity and quality. Most of the present exosome isolation approaches based on ultracentrifugation (UC), ultrafiltration (UF) or precipitation, are inefficient and hard to standardize, thereby creating low reproducibility in sample quality and potentially misleading results because highly sensitivity downstream analytical techniques, such as mass spectrometry, can detect even minute traces of co-isolated contaminants. Furthermore, loss of certain exosomal fractions during purification process or damage of exosomal membrane integrity can also alter final protein and RNA profiles. As a consequence, there is a strong interest in consensus principles for the exosome purification and the search for reliable methods for selective isolation of EV sub-populations. In the present manuscript, we critically overview the most commonly used techniques used for exosome preparation such as ultracentrifugation, size-based isolation methods, precipitation and immunoaffinity (IA) and their respective applicability for purification of exosomes from clinically relevant samples.",
publisher = "Ame Publ Co, Shatin",
journal = "TRANSLATIONAL CANCER RESEARCH",
title = "Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy",
volume = "7",
doi = "10.21037/tcr.2017.08.44"
}

Popović, M. M.,& de Marco, A.. (2018). Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy. in TRANSLATIONAL CANCER RESEARCH
Ame Publ Co, Shatin., 7.
https://doi.org/10.21037/tcr.2017.08.44

Popović MM, de Marco A. Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy. in TRANSLATIONAL CANCER RESEARCH. 2018;7.
doi:10.21037/tcr.2017.08.44 .

Popović, Milica M., de Marco, Ario, "Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy" in TRANSLATIONAL CANCER RESEARCH, 7 (2018),
https://doi.org/10.21037/tcr.2017.08.44 . .

TY  - DATA
AU  - Popović, Milica M.
AU  - Mazzega, Elisa
AU  - Toffoletto, Barbara
AU  - de Marco, Ario
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3233
PB  - Biomed Central Ltd, London
T2  - Microbial Cell Factories
T1  - Supplementary material for the article: Popovic, M.; Mazzega, E.; Toffoletto, B.; de Marco, A. Isolation of Anti-Extra-Cellular Vesicle Single-Domain Antibodies by Direct Panning on Vesicle-Enriched Fractions. Microbial cell factories 2018, 17 (1), 6. https://doi.org/10.1186/s12934-017-0856-9
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3233
ER  - 

@misc{
author = "Popović, Milica M. and Mazzega, Elisa and Toffoletto, Barbara and de Marco, Ario",
year = "2018",
publisher = "Biomed Central Ltd, London",
journal = "Microbial Cell Factories",
title = "Supplementary material for the article: Popovic, M.; Mazzega, E.; Toffoletto, B.; de Marco, A. Isolation of Anti-Extra-Cellular Vesicle Single-Domain Antibodies by Direct Panning on Vesicle-Enriched Fractions. Microbial cell factories 2018, 17 (1), 6. https://doi.org/10.1186/s12934-017-0856-9",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3233"
}

Popović, M. M., Mazzega, E., Toffoletto, B.,& de Marco, A.. (2018). Supplementary material for the article: Popovic, M.; Mazzega, E.; Toffoletto, B.; de Marco, A. Isolation of Anti-Extra-Cellular Vesicle Single-Domain Antibodies by Direct Panning on Vesicle-Enriched Fractions. Microbial cell factories 2018, 17 (1), 6. https://doi.org/10.1186/s12934-017-0856-9. in Microbial Cell Factories
Biomed Central Ltd, London..
https://hdl.handle.net/21.15107/rcub_cherry_3233

Popović MM, Mazzega E, Toffoletto B, de Marco A. Supplementary material for the article: Popovic, M.; Mazzega, E.; Toffoletto, B.; de Marco, A. Isolation of Anti-Extra-Cellular Vesicle Single-Domain Antibodies by Direct Panning on Vesicle-Enriched Fractions. Microbial cell factories 2018, 17 (1), 6. https://doi.org/10.1186/s12934-017-0856-9. in Microbial Cell Factories. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3233 .

Popović, Milica M., Mazzega, Elisa, Toffoletto, Barbara, de Marco, Ario, "Supplementary material for the article: Popovic, M.; Mazzega, E.; Toffoletto, B.; de Marco, A. Isolation of Anti-Extra-Cellular Vesicle Single-Domain Antibodies by Direct Panning on Vesicle-Enriched Fractions. Microbial cell factories 2018, 17 (1), 6. https://doi.org/10.1186/s12934-017-0856-9" in Microbial Cell Factories (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3233 .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Gregori, Marco
AU  - Vodnik, Dominik
AU  - Ferlan, Mitja
AU  - Mrak, Tanja
AU  - Straus, Ines
AU  - McDowell, Nathan G.
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2546
AB  - Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)
VL  - 47
IS  - 11
SP  - 1517
EP  - 1526
DO  - 10.1139/cjfr-2016-0530
ER  - 

@article{
author = "Popović, Milica M. and Gregori, Marco and Vodnik, Dominik and Ferlan, Mitja and Mrak, Tanja and Straus, Ines and McDowell, Nathan G. and Kraigher, Hojka and de Marco, Ario",
year = "2017",
abstract = "Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)",
volume = "47",
number = "11",
pages = "1517-1526",
doi = "10.1139/cjfr-2016-0530"
}

Popović, M. M., Gregori, M., Vodnik, D., Ferlan, M., Mrak, T., Straus, I., McDowell, N. G., Kraigher, H.,& de Marco, A.. (2017). Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 47(11), 1517-1526.
https://doi.org/10.1139/cjfr-2016-0530

Popović MM, Gregori M, Vodnik D, Ferlan M, Mrak T, Straus I, McDowell NG, Kraigher H, de Marco A. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2017;47(11):1517-1526.
doi:10.1139/cjfr-2016-0530 .

Popović, Milica M., Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Straus, Ines, McDowell, Nathan G., Kraigher, Hojka, de Marco, Ario, "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 47, no. 11 (2017):1517-1526,
https://doi.org/10.1139/cjfr-2016-0530 . .

TY  - JOUR
AU  - Kojadinović, Milica I.
AU  - Arsic, Aleksandra
AU  - Petovic-Oggiano, Gordana
AU  - Gavrović-Jankulović, Marija
AU  - Glibetic, Marija
AU  - Popović, Milica M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2551
AB  - Urolithins (UROs) are metabolites derived from ellagic acid (EA) and ellagitannins (ETs) by gut microbiota after consumption of different ETs. The health effects attributed to UROs are numerous and diverse, ranging from antimalarial properties to anticancer activities and regulation of gene expression. The aim of this work was at assessing the effect of URO-A; -B; -C; -D on the oxidative status of colon epithelium using as a model colorectal adenocarcinoma cell line (Caco-2). No significant cytotoxic effects of UROs was noted, with the applied treatments. Supplementation of cell growth medium with a mixture of UROs decreased the level of intracellular reactive oxygen species both after short- and long-term exposure. UROs also affected the activity of antioxidative enzymes within the cell, especially catalase.Conclusions: At concentrations reached in the lumen of the gut, UROs can exert beneficial effects on the cells by decreasing oxidative stress thus preventing the damage caused by reactive oxygen species.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Food Sciences and Nutrition
T1  - Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2
VL  - 68
IS  - 8
SP  - 952
EP  - 959
DO  - 10.1080/09637486.2017.1328665
ER  - 

@article{
author = "Kojadinović, Milica I. and Arsic, Aleksandra and Petovic-Oggiano, Gordana and Gavrović-Jankulović, Marija and Glibetic, Marija and Popović, Milica M.",
year = "2017",
abstract = "Urolithins (UROs) are metabolites derived from ellagic acid (EA) and ellagitannins (ETs) by gut microbiota after consumption of different ETs. The health effects attributed to UROs are numerous and diverse, ranging from antimalarial properties to anticancer activities and regulation of gene expression. The aim of this work was at assessing the effect of URO-A; -B; -C; -D on the oxidative status of colon epithelium using as a model colorectal adenocarcinoma cell line (Caco-2). No significant cytotoxic effects of UROs was noted, with the applied treatments. Supplementation of cell growth medium with a mixture of UROs decreased the level of intracellular reactive oxygen species both after short- and long-term exposure. UROs also affected the activity of antioxidative enzymes within the cell, especially catalase.Conclusions: At concentrations reached in the lumen of the gut, UROs can exert beneficial effects on the cells by decreasing oxidative stress thus preventing the damage caused by reactive oxygen species.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Food Sciences and Nutrition",
title = "Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2",
volume = "68",
number = "8",
pages = "952-959",
doi = "10.1080/09637486.2017.1328665"
}

Kojadinović, M. I., Arsic, A., Petovic-Oggiano, G., Gavrović-Jankulović, M., Glibetic, M.,& Popović, M. M.. (2017). Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2. in International Journal of Food Sciences and Nutrition
Taylor & Francis Ltd, Abingdon., 68(8), 952-959.
https://doi.org/10.1080/09637486.2017.1328665

Kojadinović MI, Arsic A, Petovic-Oggiano G, Gavrović-Jankulović M, Glibetic M, Popović MM. Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2. in International Journal of Food Sciences and Nutrition. 2017;68(8):952-959.
doi:10.1080/09637486.2017.1328665 .

Kojadinović, Milica I., Arsic, Aleksandra, Petovic-Oggiano, Gordana, Gavrović-Jankulović, Marija, Glibetic, Marija, Popović, Milica M., "Effect of urolithins on oxidative stress of colorectal adenocarcinomacells-Caco-2" in International Journal of Food Sciences and Nutrition, 68, no. 8 (2017):952-959,
https://doi.org/10.1080/09637486.2017.1328665 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Tufegdžić, Srđan
AU  - Popović, Milica M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2557
AB  - The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.
PB  - Wiley, Hoboken
T2  - Electrophoresis
T1  - Use of monolithic supports for high-throughput protein and peptide separation in proteomics
VL  - 38
IS  - 22-23
SP  - 2851
EP  - 2869
DO  - 10.1002/elps.201700221
ER  - 

@article{
author = "Anđelković, Uroš and Tufegdžić, Srđan and Popović, Milica M.",
year = "2017",
abstract = "The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.",
publisher = "Wiley, Hoboken",
journal = "Electrophoresis",
title = "Use of monolithic supports for high-throughput protein and peptide separation in proteomics",
volume = "38",
number = "22-23",
pages = "2851-2869",
doi = "10.1002/elps.201700221"
}

Anđelković, U., Tufegdžić, S.,& Popović, M. M.. (2017). Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis
Wiley, Hoboken., 38(22-23), 2851-2869.
https://doi.org/10.1002/elps.201700221

Anđelković U, Tufegdžić S, Popović MM. Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis. 2017;38(22-23):2851-2869.
doi:10.1002/elps.201700221 .

Anđelković, Uroš, Tufegdžić, Srđan, Popović, Milica M., "Use of monolithic supports for high-throughput protein and peptide separation in proteomics" in Electrophoresis, 38, no. 22-23 (2017):2851-2869,
https://doi.org/10.1002/elps.201700221 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Gregori, Marco
AU  - Vodnik, Dominik
AU  - Ferlan, Mitja
AU  - Mrak, Tanja
AU  - Straus, Ines
AU  - McDowell, Nathan G.
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2973
AB  - Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)
VL  - 47
IS  - 11
SP  - 1517
EP  - 1526
DO  - 10.1139/cjfr-2016-0530
ER  - 

@article{
author = "Popović, Milica M. and Gregori, Marco and Vodnik, Dominik and Ferlan, Mitja and Mrak, Tanja and Straus, Ines and McDowell, Nathan G. and Kraigher, Hojka and de Marco, Ario",
year = "2017",
abstract = "Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)",
volume = "47",
number = "11",
pages = "1517-1526",
doi = "10.1139/cjfr-2016-0530"
}

Popović, M. M., Gregori, M., Vodnik, D., Ferlan, M., Mrak, T., Straus, I., McDowell, N. G., Kraigher, H.,& de Marco, A.. (2017). Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 47(11), 1517-1526.
https://doi.org/10.1139/cjfr-2016-0530

Popović MM, Gregori M, Vodnik D, Ferlan M, Mrak T, Straus I, McDowell NG, Kraigher H, de Marco A. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2017;47(11):1517-1526.
doi:10.1139/cjfr-2016-0530 .

Popović, Milica M., Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Straus, Ines, McDowell, Nathan G., Kraigher, Hojka, de Marco, Ario, "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 47, no. 11 (2017):1517-1526,
https://doi.org/10.1139/cjfr-2016-0530 . .

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Tufegdžić, Srđan
AU  - Popović, Milica M.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2976
AB  - The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.
PB  - Wiley, Hoboken
T2  - Electrophoresis
T1  - Use of monolithic supports for high-throughput protein and peptide separation in proteomics
VL  - 38
IS  - 22-23
SP  - 2851
EP  - 2869
DO  - 10.1002/elps.201700221
ER  - 

@article{
author = "Anđelković, Uroš and Tufegdžić, Srđan and Popović, Milica M.",
year = "2017",
abstract = "The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.",
publisher = "Wiley, Hoboken",
journal = "Electrophoresis",
title = "Use of monolithic supports for high-throughput protein and peptide separation in proteomics",
volume = "38",
number = "22-23",
pages = "2851-2869",
doi = "10.1002/elps.201700221"
}

Anđelković, U., Tufegdžić, S.,& Popović, M. M.. (2017). Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis
Wiley, Hoboken., 38(22-23), 2851-2869.
https://doi.org/10.1002/elps.201700221

Anđelković U, Tufegdžić S, Popović MM. Use of monolithic supports for high-throughput protein and peptide separation in proteomics. in Electrophoresis. 2017;38(22-23):2851-2869.
doi:10.1002/elps.201700221 .

Anđelković, Uroš, Tufegdžić, Srđan, Popović, Milica M., "Use of monolithic supports for high-throughput protein and peptide separation in proteomics" in Electrophoresis, 38, no. 22-23 (2017):2851-2869,
https://doi.org/10.1002/elps.201700221 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Sustar, Vid
AU  - Gricar, Jozica
AU  - Straus, Ines
AU  - Torkar, Gregor
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2049
AB  - Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)
VL  - 46
IS  - 1
SP  - 58
EP  - 66
DO  - 10.1139/cjfr-2015-0274
ER  - 

@article{
author = "Popović, Milica M. and Sustar, Vid and Gricar, Jozica and Straus, Ines and Torkar, Gregor and Kraigher, Hojka and de Marco, Ario",
year = "2016",
abstract = "Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)",
volume = "46",
number = "1",
pages = "58-66",
doi = "10.1139/cjfr-2015-0274"
}

Popović, M. M., Sustar, V., Gricar, J., Straus, I., Torkar, G., Kraigher, H.,& de Marco, A.. (2016). Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 46(1), 58-66.
https://doi.org/10.1139/cjfr-2015-0274

Popović MM, Sustar V, Gricar J, Straus I, Torkar G, Kraigher H, de Marco A. Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2016;46(1):58-66.
doi:10.1139/cjfr-2015-0274 .

Popović, Milica M., Sustar, Vid, Gricar, Jozica, Straus, Ines, Torkar, Gregor, Kraigher, Hojka, de Marco, Ario, "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 46, no. 1 (2016):58-66,
https://doi.org/10.1139/cjfr-2015-0274 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Sustar, Vid
AU  - Gricar, Jozica
AU  - Straus, Ines
AU  - Torkar, Gregor
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3530
AB  - Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)
VL  - 46
IS  - 1
SP  - 58
EP  - 66
DO  - 10.1139/cjfr-2015-0274
ER  - 

@article{
author = "Popović, Milica M. and Sustar, Vid and Gricar, Jozica and Straus, Ines and Torkar, Gregor and Kraigher, Hojka and de Marco, Ario",
year = "2016",
abstract = "Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)",
volume = "46",
number = "1",
pages = "58-66",
doi = "10.1139/cjfr-2015-0274"
}

Popović, M. M., Sustar, V., Gricar, J., Straus, I., Torkar, G., Kraigher, H.,& de Marco, A.. (2016). Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 46(1), 58-66.
https://doi.org/10.1139/cjfr-2015-0274

Popović MM, Sustar V, Gricar J, Straus I, Torkar G, Kraigher H, de Marco A. Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2016;46(1):58-66.
doi:10.1139/cjfr-2015-0274 .

Popović, Milica M., Sustar, Vid, Gricar, Jozica, Straus, Ines, Torkar, Gregor, Kraigher, Hojka, de Marco, Ario, "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 46, no. 1 (2016):58-66,
https://doi.org/10.1139/cjfr-2015-0274 . .

TY  - CHAP
AU  - Nikolić, Jasna
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/314
AB  - Banana is a perennial herbaceous plant widely cultivated in the tropical and subtropical regions. The pulp of the fruit is a rich source of minerals, vitamins, antioxidants, low glycemic carbohydrates, and fiber, and thereby its consumption has beneficial effects on human health. These nutritional values and its pleasant taste induced the introduction of banana fruit into human diet in infancy and also during convalescence. However, in spite of positive health effects, banana fruit has been recognized as an important food allergen source. The clinical manifestations of banana allergy have usually been associated with mild, local symptoms denoted as oral allergy syndrome. However, more severe reactions, as well as cases of anaphylactic reactions to banana fruit have been registered. IgE reactivity of banana is associated with different proteins, and, so far, only six allergens have been identified and characterized: profilin - actin binding protein (Mus a 1), a class 1 chitinase (Mus a 2), non-specific lipid transfer protein (Mus a 3), thaumatin-like protein (Mus a 4), beta-1,3-glucanase (Mus a 5), and recently registered ascorbate peroxidase (Mus a 6). In this review, we will address the structural features of identified banana allergens and correlate in vitro and in vivo clinical reactivity with their structural homologs from other allergen sources. © 2016 Nova Science Publishers, Inc.
T2  - Bananas: Cultivation, Consumption and Crop Diseases
T1  - Banana as a food allergen source
SP  - 107
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_cherry_314
ER  - 

@inbook{
author = "Nikolić, Jasna and Popović, Milica M. and Gavrović-Jankulović, Marija",
year = "2016",
abstract = "Banana is a perennial herbaceous plant widely cultivated in the tropical and subtropical regions. The pulp of the fruit is a rich source of minerals, vitamins, antioxidants, low glycemic carbohydrates, and fiber, and thereby its consumption has beneficial effects on human health. These nutritional values and its pleasant taste induced the introduction of banana fruit into human diet in infancy and also during convalescence. However, in spite of positive health effects, banana fruit has been recognized as an important food allergen source. The clinical manifestations of banana allergy have usually been associated with mild, local symptoms denoted as oral allergy syndrome. However, more severe reactions, as well as cases of anaphylactic reactions to banana fruit have been registered. IgE reactivity of banana is associated with different proteins, and, so far, only six allergens have been identified and characterized: profilin - actin binding protein (Mus a 1), a class 1 chitinase (Mus a 2), non-specific lipid transfer protein (Mus a 3), thaumatin-like protein (Mus a 4), beta-1,3-glucanase (Mus a 5), and recently registered ascorbate peroxidase (Mus a 6). In this review, we will address the structural features of identified banana allergens and correlate in vitro and in vivo clinical reactivity with their structural homologs from other allergen sources. © 2016 Nova Science Publishers, Inc.",
journal = "Bananas: Cultivation, Consumption and Crop Diseases",
booktitle = "Banana as a food allergen source",
pages = "107-126",
url = "https://hdl.handle.net/21.15107/rcub_cherry_314"
}

Nikolić, J., Popović, M. M.,& Gavrović-Jankulović, M.. (2016). Banana as a food allergen source. in Bananas: Cultivation, Consumption and Crop Diseases, 107-126.
https://hdl.handle.net/21.15107/rcub_cherry_314

Nikolić J, Popović MM, Gavrović-Jankulović M. Banana as a food allergen source. in Bananas: Cultivation, Consumption and Crop Diseases. 2016;:107-126.
https://hdl.handle.net/21.15107/rcub_cherry_314 .

Nikolić, Jasna, Popović, Milica M., Gavrović-Jankulović, Marija, "Banana as a food allergen source" in Bananas: Cultivation, Consumption and Crop Diseases (2016):107-126,
https://hdl.handle.net/21.15107/rcub_cherry_314 .

TY  - JOUR
AU  - Rašković, Brankica
AU  - Popović, Milica M.
AU  - Ostojić, Sanja B.
AU  - Anđelković, Boban D.
AU  - Tešević, Vele
AU  - Polović, Natalija
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3367
AB  - Papain is a cysteine protease with wide substrate specificity and many applications. Despite its widespread applications, cold stability of papain has never been studied. Here, we used differential spectroscopy to monitor thermal denaturation process. Papain was the most stabile from 45 degrees C to 60 degrees C with Delta G degrees(321) of 13.9 +/- 0.3 kJ/mol and T-m value of 84 +/- 1 degrees C. After cold storage, papain lost parts of its native secondary structures elements which gave an increase of 40% of intermolecular beta-sheet content (band maximum detected at frequency of 1621 cm(-1) in Fourier transform infrared (FT-IR) spectrum) indicating the presence of secondary structures necessary for aggregation. The presence of protein aggregates after cold storage was also proven by analytical size exclusion chromatography. After six freeze-thaw cycles around 75% of starting enzyme activity of papain was lost due to cold denaturation and aggregation of unfolded protein. Autoproteolysis of papain did not cause significant loss of the protein activity. Upon the cold storage, papain underwent structural rearrangements and aggregation that correspond to other cold denatured proteins, rather than autoproteolysis which could have the commercial importance for the growing polypeptide based industry.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy
T1  - Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage
VL  - 150
SP  - 238
EP  - 246
DO  - 10.1016/j.saa.2015.05.061
ER  - 

@article{
author = "Rašković, Brankica and Popović, Milica M. and Ostojić, Sanja B. and Anđelković, Boban D. and Tešević, Vele and Polović, Natalija",
year = "2015",
abstract = "Papain is a cysteine protease with wide substrate specificity and many applications. Despite its widespread applications, cold stability of papain has never been studied. Here, we used differential spectroscopy to monitor thermal denaturation process. Papain was the most stabile from 45 degrees C to 60 degrees C with Delta G degrees(321) of 13.9 +/- 0.3 kJ/mol and T-m value of 84 +/- 1 degrees C. After cold storage, papain lost parts of its native secondary structures elements which gave an increase of 40% of intermolecular beta-sheet content (band maximum detected at frequency of 1621 cm(-1) in Fourier transform infrared (FT-IR) spectrum) indicating the presence of secondary structures necessary for aggregation. The presence of protein aggregates after cold storage was also proven by analytical size exclusion chromatography. After six freeze-thaw cycles around 75% of starting enzyme activity of papain was lost due to cold denaturation and aggregation of unfolded protein. Autoproteolysis of papain did not cause significant loss of the protein activity. Upon the cold storage, papain underwent structural rearrangements and aggregation that correspond to other cold denatured proteins, rather than autoproteolysis which could have the commercial importance for the growing polypeptide based industry.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy",
title = "Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage",
volume = "150",
pages = "238-246",
doi = "10.1016/j.saa.2015.05.061"
}

Rašković, B., Popović, M. M., Ostojić, S. B., Anđelković, B. D., Tešević, V.,& Polović, N.. (2015). Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage. in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 150, 238-246.
https://doi.org/10.1016/j.saa.2015.05.061

Rašković B, Popović MM, Ostojić SB, Anđelković BD, Tešević V, Polović N. Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage. in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy. 2015;150:238-246.
doi:10.1016/j.saa.2015.05.061 .

Rašković, Brankica, Popović, Milica M., Ostojić, Sanja B., Anđelković, Boban D., Tešević, Vele, Polović, Natalija, "Fourier transform infrared spectroscopy provides an evidence of papain denaturation and aggregation during cold storage" in Spectrochimica Acta. Part A: Molecular and Biomolecular Spectroscopy, 150 (2015):238-246,
https://doi.org/10.1016/j.saa.2015.05.061 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Prodanović, Radivoje
AU  - Ostafe, Raluca
AU  - Schillberg, Stefan
AU  - Fischer, Rainer
AU  - Gavrović-Jankulović, Marija
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1668
AB  - High-throughput characterization of allergens relies often on phage display technique which is subject to the limitations of a prokaryotic expression system. Substituting the phage display platform with a yeast surface display could lead to fast immunological characterization of allergens with complex structures. Our objective was to evaluate the potential of yeast surface display for characterization of plant-derived food allergens. The coding sequence of mature actinidin (Act d 1) was cloned into pCTCON2 surface display vector. Flow cytometry was used to confirm localization of recombinant Act d 1 on the surface of yeast cells using rabbit polyclonal antisera IgG and IgE from sera of kiwifruit-allergic individuals. Immunological (dot blot, immunoblot ELISA and ELISA inhibition), biochemical (enzymatic activity in gel) and biological (basophil activation) characterization of Act d 1 after solubilization from the yeast cell confirmed that recombinant Act d 1 produced on the surface of yeast cell is similar to its natural counterpart isolated from green kiwifruit. Yeast surface display is a potent technique that enables fast immunochemical characterization of allergens in situ without the need for protein purification and offers an alternative that could lead to improvement of standard immunodiagnostic and immunotherapeutic approaches.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens
VL  - 61
IS  - 3
SP  - 230
EP  - 239
DO  - 10.1007/s12026-014-8614-0
ER  - 

@article{
author = "Popović, Milica M. and Prodanović, Radivoje and Ostafe, Raluca and Schillberg, Stefan and Fischer, Rainer and Gavrović-Jankulović, Marija",
year = "2015",
abstract = "High-throughput characterization of allergens relies often on phage display technique which is subject to the limitations of a prokaryotic expression system. Substituting the phage display platform with a yeast surface display could lead to fast immunological characterization of allergens with complex structures. Our objective was to evaluate the potential of yeast surface display for characterization of plant-derived food allergens. The coding sequence of mature actinidin (Act d 1) was cloned into pCTCON2 surface display vector. Flow cytometry was used to confirm localization of recombinant Act d 1 on the surface of yeast cells using rabbit polyclonal antisera IgG and IgE from sera of kiwifruit-allergic individuals. Immunological (dot blot, immunoblot ELISA and ELISA inhibition), biochemical (enzymatic activity in gel) and biological (basophil activation) characterization of Act d 1 after solubilization from the yeast cell confirmed that recombinant Act d 1 produced on the surface of yeast cell is similar to its natural counterpart isolated from green kiwifruit. Yeast surface display is a potent technique that enables fast immunochemical characterization of allergens in situ without the need for protein purification and offers an alternative that could lead to improvement of standard immunodiagnostic and immunotherapeutic approaches.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens",
volume = "61",
number = "3",
pages = "230-239",
doi = "10.1007/s12026-014-8614-0"
}

Popović, M. M., Prodanović, R., Ostafe, R., Schillberg, S., Fischer, R.,& Gavrović-Jankulović, M.. (2015). Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. in Immunologic Research
Humana Press Inc, Totowa., 61(3), 230-239.
https://doi.org/10.1007/s12026-014-8614-0

Popović MM, Prodanović R, Ostafe R, Schillberg S, Fischer R, Gavrović-Jankulović M. Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. in Immunologic Research. 2015;61(3):230-239.
doi:10.1007/s12026-014-8614-0 .

Popović, Milica M., Prodanović, Radivoje, Ostafe, Raluca, Schillberg, Stefan, Fischer, Rainer, Gavrović-Jankulović, Marija, "Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens" in Immunologic Research, 61, no. 3 (2015):230-239,
https://doi.org/10.1007/s12026-014-8614-0 . .