Mandić, Ljuba M.

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Authority KeyName Variants
orcid::0000-0002-6620-1230
  • Mandić, Ljuba M. (56)
Projects
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research
FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology
Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology
Theory and practice of science in society: multidisciplinary, educational and intergenerational perspectives Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima
FP7 RegPot FCUB-ERA, GA High-Performance Computing Infrastructure for South East Europe's Research Communities
Development of new and improvement of existing electrochemical, spectroscopic and flow injection (FIA) methods on environmental quality monitoring Molecular mechanisms of cellular responses on pathological changes in central neuronal system and peripheral organs of mammals
An integral study to identify the regional genetic and environmental risk factors for the common noncommunicable diseases in the human population of Serbia - INGEMA_S Traditional and new products of cultivated and wild growing fruits and grape vines, and by-products durring processing, with special emphasis on indigenous varieties: chemical characterization and biological profile
Razvoj novih terapijskih postupaka u prevenciji i lečenju bolesti jetre: Uloga i mehanizam delovanja polinezasićenih masnih kiselina

Author's Bibliography

Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content

Uzelac, Tamara N.; Nikolić-Kokić, Aleksandra; Spasić, Snežana; Mačvanin, Mirjana T.; Nikolić, Milan; Mandić, Ljuba M.; Jovanović, Vesna B.

(2019)

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3333
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
SP  - 1
EP  - 7
DO  - 10.1016/j.cbi.2019.108787
ER  - 
@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
pages = "1-7",
doi = "10.1016/j.cbi.2019.108787"
}
Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions, 311, 1-7.
https://doi.org/10.1016/j.cbi.2019.108787
Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311:1-7.
doi:10.1016/j.cbi.2019.108787 .
Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311 (2019):1-7,
https://doi.org/10.1016/j.cbi.2019.108787 . .

Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787

Uzelac, Tamara N.; Nikolić-Kokić, Aleksandra; Spasić, Snežana; Mačvanin, Mirjana T.; Nikolić, Milan; Mandić, Ljuba M.; Jovanović, Vesna B.

(2019)

TY  - DATA
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3335
T2  - Chemico-Biological Interactions
T1  - Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3335
ER  - 
@misc{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
journal = "Chemico-Biological Interactions",
title = "Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3335"
}
Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787. in Chemico-Biological Interactions.
https://hdl.handle.net/21.15107/rcub_cherry_3335
Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787. in Chemico-Biological Interactions. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3335 .
Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Supplementary data for the article: Uzelac, T. N.; Nikolić-Kokić, A. L.; Spasić, S. D.; Mačvanin, M. T.; Nikolić, M. R.; Mandić, L. M.; Jovanović, V. B. Opposite Clozapine and Ziprasidone Effects on the Reactivity of Plasma Albumin SH-Group Are the Consequence of Their Different Binding Properties Dependent on Protein Fatty Acids Content. Chemico-Biological Interactions 2019, 311. https://doi.org/10.1016/j.cbi.2019.108787" in Chemico-Biological Interactions (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3335 .

Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content

Uzelac, Tamara N.; Nikolić-Kokić, Aleksandra; Spasić, Snežana; Mačvanin, Mirjana T.; Nikolić, Milan; Mandić, Ljuba M.; Jovanović, Vesna B.

(Elsevier, 2019)

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3865
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
IS  - 108787
DO  - 10.1016/j.cbi.2019.108787
ER  - 
@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
number = "108787",
doi = "10.1016/j.cbi.2019.108787"
}
Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions
Elsevier., 311(108787).
https://doi.org/10.1016/j.cbi.2019.108787
Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311(108787).
doi:10.1016/j.cbi.2019.108787 .
Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311, no. 108787 (2019),
https://doi.org/10.1016/j.cbi.2019.108787 . .

Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409

Đekić-Ivanković, Marija; Weiler, Hope; Jones, Glenville; Kaufmann, Martin; Kaluđerović, Jovana; Aleksić-Veličković, Vesna; Mandić, Ljuba M.; Glibetic, Maria

(Cambridge Univ Press, Cambridge, 2017)

TY  - DATA
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Jones, Glenville
AU  - Kaufmann, Martin
AU  - Kaluđerović, Jovana
AU  - Aleksić-Veličković, Vesna
AU  - Mandić, Ljuba M.
AU  - Glibetic, Maria
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3262
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3262
ER  - 
@misc{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Jones, Glenville and Kaufmann, Martin and Kaluđerović, Jovana and Aleksić-Veličković, Vesna and Mandić, Ljuba M. and Glibetic, Maria",
year = "2017",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3262"
}
Đekić-Ivanković, M., Weiler, H., Jones, G., Kaufmann, M., Kaluđerović, J., Aleksić-Veličković, V., Mandić, L. M.,& Glibetic, M.. (2017). Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409. in Public Health Nutrition
Cambridge Univ Press, Cambridge..
https://hdl.handle.net/21.15107/rcub_cherry_3262
Đekić-Ivanković M, Weiler H, Jones G, Kaufmann M, Kaluđerović J, Aleksić-Veličković V, Mandić LM, Glibetic M. Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409. in Public Health Nutrition. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_3262 .
Đekić-Ivanković, Marija, Weiler, Hope, Jones, Glenville, Kaufmann, Martin, Kaluđerović, Jovana, Aleksić-Veličković, Vesna, Mandić, Ljuba M., Glibetic, Maria, "Supplementary material for the article: Djekic-Ivankovic, M.; Weiler, H.; Jones, G.; Kaufmann, M.; Kaludjerovic, J.; Aleksic-Velickovic, V.; Mandić, L. M.; Glibetic, M. Vitamin D Status in Mothers with Pre-Eclampsia and Their Infants: A Case-Control Study from Serbia, a Country without a Vitamin D Fortification Policy. Public Health Nutrition 2017, 20 (10), 1825–1835. https://doi.org/10.1017/S1368980016000409" in Public Health Nutrition (2017),
https://hdl.handle.net/21.15107/rcub_cherry_3262 .

Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy

Đekić-Ivanković, Marija; Weiler, Hope; Jones, Glenville; Kaufmann, Martin; Kaluđerović, Jovana; Aleksić-Veličković, Vesna; Mandić, Ljuba M.; Glibetic, Maria

(Cambridge Univ Press, Cambridge, 2017)

TY  - JOUR
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Jones, Glenville
AU  - Kaufmann, Martin
AU  - Kaluđerović, Jovana
AU  - Aleksić-Veličković, Vesna
AU  - Mandić, Ljuba M.
AU  - Glibetic, Maria
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2161
AB  - Objective: The objective of the present study was to determine if vitamin D intake and status are associated with pre-eclampsia in a country without a vitamin D fortification policy. Design: A case-control study of pregnancies with (case) and without (control) pre-eclampsia was conducted from January to April when UVB is minimal. Maternal and cord blood obtained at delivery were measured for plasma 25-hydroxycholecalciferol (25-OH-D-3), 3-epimer of 25-OH-D-3 (3-epi-25-OH-D-3) and 24,25-dihydroxycholecalciferol (24,25-(OH)(2)D-3) by LC-MS/MS and maternal 1,25-dihydroxyvitamin D (1,25-(OH) 2D). Differences between groups were tested with ANOVA and Bonferroni post hoc tests (P  lt  0.05). Setting: Clinical Center of Serbia. Subjects: Pregnant women with and without pre-eclampsia (n 60) and their infants. Results: Exogenous vitamin D intake (0.95-16.25 mu g/d (38-650 IU/d)) was not significantly different between groups. Women with pre-eclampsia delivered infants at an earlier gestational age and had significantly lower mean total plasma 25-hydroxyvitamin D (25-OH-D; case: 11.2 (SD 5.1); control: 16.1 (SD 5.7) ng/ml; P=0.0006), 25-OH-D-3 (case: 10.0 (SD 4.9); control: 14.2 (SD 5.8) ng/ml; P=0.002), 3-epi-25-OH-D-3 (case: 0.5 (SD 0.2); control: 0.7 (SD 0.2) ng/ml; P=0.0007) and 1,25-(OH)(2)D (case: 56.5 (SD 26.6); control: 81.0 (SD 25.7) pg/ml; P=0.018), while 24,25-(OH)(2)D-3 was not different between groups. Infants did not differ in total plasma 25-OH-D, 25-OH-D-3, 3-epi-25-OH-D-3 and 24,25-(OH)(2)D-3, but the mean proportion of 3-epi-25-OH-D-3 was higher in the infant case group (case: 7.9 (SD 1.1); control: 7.0 (SD 1.4) % of total 25-OH-D-3; P=0.005). Conclusions: A high prevalence of vitamin D deficiency, as defined by plasma 25-OH-D lt 12 ng/ml, was observed in 47 % of all mothers and 77 % of all infants. These data underscore the need for prenatal vitamin D supplementation and a food fortification policy in Serbia.
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy
VL  - 20
IS  - 10
SP  - 1825
EP  - 1835
DO  - 10.1017/S1368980016000409
UR  - Kon_3492
ER  - 
@article{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Jones, Glenville and Kaufmann, Martin and Kaluđerović, Jovana and Aleksić-Veličković, Vesna and Mandić, Ljuba M. and Glibetic, Maria",
year = "2017",
abstract = "Objective: The objective of the present study was to determine if vitamin D intake and status are associated with pre-eclampsia in a country without a vitamin D fortification policy. Design: A case-control study of pregnancies with (case) and without (control) pre-eclampsia was conducted from January to April when UVB is minimal. Maternal and cord blood obtained at delivery were measured for plasma 25-hydroxycholecalciferol (25-OH-D-3), 3-epimer of 25-OH-D-3 (3-epi-25-OH-D-3) and 24,25-dihydroxycholecalciferol (24,25-(OH)(2)D-3) by LC-MS/MS and maternal 1,25-dihydroxyvitamin D (1,25-(OH) 2D). Differences between groups were tested with ANOVA and Bonferroni post hoc tests (P  lt  0.05). Setting: Clinical Center of Serbia. Subjects: Pregnant women with and without pre-eclampsia (n 60) and their infants. Results: Exogenous vitamin D intake (0.95-16.25 mu g/d (38-650 IU/d)) was not significantly different between groups. Women with pre-eclampsia delivered infants at an earlier gestational age and had significantly lower mean total plasma 25-hydroxyvitamin D (25-OH-D; case: 11.2 (SD 5.1); control: 16.1 (SD 5.7) ng/ml; P=0.0006), 25-OH-D-3 (case: 10.0 (SD 4.9); control: 14.2 (SD 5.8) ng/ml; P=0.002), 3-epi-25-OH-D-3 (case: 0.5 (SD 0.2); control: 0.7 (SD 0.2) ng/ml; P=0.0007) and 1,25-(OH)(2)D (case: 56.5 (SD 26.6); control: 81.0 (SD 25.7) pg/ml; P=0.018), while 24,25-(OH)(2)D-3 was not different between groups. Infants did not differ in total plasma 25-OH-D, 25-OH-D-3, 3-epi-25-OH-D-3 and 24,25-(OH)(2)D-3, but the mean proportion of 3-epi-25-OH-D-3 was higher in the infant case group (case: 7.9 (SD 1.1); control: 7.0 (SD 1.4) % of total 25-OH-D-3; P=0.005). Conclusions: A high prevalence of vitamin D deficiency, as defined by plasma 25-OH-D lt 12 ng/ml, was observed in 47 % of all mothers and 77 % of all infants. These data underscore the need for prenatal vitamin D supplementation and a food fortification policy in Serbia.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy",
volume = "20",
number = "10",
pages = "1825-1835",
doi = "10.1017/S1368980016000409",
url = "Kon_3492"
}
Đekić-Ivanković, M., Weiler, H., Jones, G., Kaufmann, M., Kaluđerović, J., Aleksić-Veličković, V., Mandić, L. M.,& Glibetic, M.. (2017). Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy. in Public Health Nutrition
Cambridge Univ Press, Cambridge., 20(10), 1825-1835.
https://doi.org/10.1017/S1368980016000409
Kon_3492
Đekić-Ivanković M, Weiler H, Jones G, Kaufmann M, Kaluđerović J, Aleksić-Veličković V, Mandić LM, Glibetic M. Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy. in Public Health Nutrition. 2017;20(10):1825-1835.
doi:10.1017/S1368980016000409
Kon_3492 .
Đekić-Ivanković, Marija, Weiler, Hope, Jones, Glenville, Kaufmann, Martin, Kaluđerović, Jovana, Aleksić-Veličković, Vesna, Mandić, Ljuba M., Glibetic, Maria, "Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy" in Public Health Nutrition, 20, no. 10 (2017):1825-1835,
https://doi.org/10.1017/S1368980016000409 .,
Kon_3492 .
14
6
10

Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal

Aćimović, Jelena M.; Penezić, Ana Z.; Pavićević, Ivan D.; Jovanović, Vesna B.; Takić, Marija M.; Uzelac, T. N.; Mandić, Ljuba M.

(Wiley-Blackwell, Hoboken, 2016)

TY  - CONF
AU  - Aćimović, Jelena M.
AU  - Penezić, Ana Z.
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija M.
AU  - Uzelac, T. N.
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2307
PB  - Wiley-Blackwell, Hoboken
C3  - FEBS Journal / Federation of European of Biochemical Societies
T1  - Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal
VL  - 283
SP  - 417
EP  - 417
UR  - Kon_3123
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2307
ER  - 
@conference{
author = "Aćimović, Jelena M. and Penezić, Ana Z. and Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija M. and Uzelac, T. N. and Mandić, Ljuba M.",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "FEBS Journal / Federation of European of Biochemical Societies",
title = "Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal",
volume = "283",
pages = "417-417",
url = "Kon_3123, https://hdl.handle.net/21.15107/rcub_cherry_2307"
}
Aćimović, J. M., Penezić, A. Z., Pavićević, I. D., Jovanović, V. B., Takić, M. M., Uzelac, T. N.,& Mandić, L. M.. (2016). Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal. in FEBS Journal / Federation of European of Biochemical Societies
Wiley-Blackwell, Hoboken., 283, 417-417.
Kon_3123
Aćimović JM, Penezić AZ, Pavićević ID, Jovanović VB, Takić MM, Uzelac TN, Mandić LM. Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal. in FEBS Journal / Federation of European of Biochemical Societies. 2016;283:417-417.
Kon_3123 .
Aćimović, Jelena M., Penezić, Ana Z., Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija M., Uzelac, T. N., Mandić, Ljuba M., "Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal" in FEBS Journal / Federation of European of Biochemical Societies, 283 (2016):417-417,
Kon_3123 .

Quantification of total content of non-esterified fatty acids bound to human serum albumin

Pavićević, Ivan D.; Jovanović, Vesna B.; Takić, Marija M.; Aćimović, Jelena M.; Penezić, Ana Z.; Mandić, Ljuba M.

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija M.
AU  - Aćimović, Jelena M.
AU  - Penezić, Ana Z.
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2399
AB  - Non-esterified fatty acids bound to the human serum albumin (HSA) contribute to several HSAs properties of special concern in pathologies, for instance to the reactivity of the free HSA-Cys34 thiol group (important antioxidative thiol pool in plasma), and to the affinity for binding of molecules and ions (for example cobalt as a prominent biomarker in heart ischemia). Therefore, the method for determination of FAs bound to HSA was developed. FAs were released from HSA (previously isolated from serum by ammonium sulfate precipitation) using acidic copper(II) sulfate in phosphoric acid, extracted by n-heptane-chloroform (4:1, v/v) mixture, spotted on TL silica-gel and then developed with n-heptane-chloroform-acetic acid (5:3:03, v/v/v). Common office flatbed scanner and software solution for densitometric image analysis, developed in R, were used. The linearity of calibration curve in concentration range from 0.1 to 5.0 mmol/L stearic acid was achieved. The method was proved to be precise (with RSD of 1.4-4.7%) and accurate. Accuracy was examined by standard addition method (recoveries 97.2-102.5%) and by comparison to results of GC. The method is sample saving, technically less demanding, and cheap, and therefore suitable for determination of FAs/HSA ratio when elevated concentrations of free FAs are reliable diagnostic/risk parameter of pathological states. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Quantification of total content of non-esterified fatty acids bound to human serum albumin
VL  - 129
SP  - 43
EP  - 49
DO  - 10.1016/j.jpba.2016.06.043
UR  - Kon_3215
ER  - 
@article{
author = "Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija M. and Aćimović, Jelena M. and Penezić, Ana Z. and Mandić, Ljuba M.",
year = "2016",
abstract = "Non-esterified fatty acids bound to the human serum albumin (HSA) contribute to several HSAs properties of special concern in pathologies, for instance to the reactivity of the free HSA-Cys34 thiol group (important antioxidative thiol pool in plasma), and to the affinity for binding of molecules and ions (for example cobalt as a prominent biomarker in heart ischemia). Therefore, the method for determination of FAs bound to HSA was developed. FAs were released from HSA (previously isolated from serum by ammonium sulfate precipitation) using acidic copper(II) sulfate in phosphoric acid, extracted by n-heptane-chloroform (4:1, v/v) mixture, spotted on TL silica-gel and then developed with n-heptane-chloroform-acetic acid (5:3:03, v/v/v). Common office flatbed scanner and software solution for densitometric image analysis, developed in R, were used. The linearity of calibration curve in concentration range from 0.1 to 5.0 mmol/L stearic acid was achieved. The method was proved to be precise (with RSD of 1.4-4.7%) and accurate. Accuracy was examined by standard addition method (recoveries 97.2-102.5%) and by comparison to results of GC. The method is sample saving, technically less demanding, and cheap, and therefore suitable for determination of FAs/HSA ratio when elevated concentrations of free FAs are reliable diagnostic/risk parameter of pathological states. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Quantification of total content of non-esterified fatty acids bound to human serum albumin",
volume = "129",
pages = "43-49",
doi = "10.1016/j.jpba.2016.06.043",
url = "Kon_3215"
}
Pavićević, I. D., Jovanović, V. B., Takić, M. M., Aćimović, J. M., Penezić, A. Z.,& Mandić, L. M.. (2016). Quantification of total content of non-esterified fatty acids bound to human serum albumin. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science Bv, Amsterdam., 129, 43-49.
https://doi.org/10.1016/j.jpba.2016.06.043
Kon_3215
Pavićević ID, Jovanović VB, Takić MM, Aćimović JM, Penezić AZ, Mandić LM. Quantification of total content of non-esterified fatty acids bound to human serum albumin. in Journal of Pharmaceutical and Biomedical Analysis. 2016;129:43-49.
doi:10.1016/j.jpba.2016.06.043
Kon_3215 .
Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija M., Aćimović, Jelena M., Penezić, Ana Z., Mandić, Ljuba M., "Quantification of total content of non-esterified fatty acids bound to human serum albumin" in Journal of Pharmaceutical and Biomedical Analysis, 129 (2016):43-49,
https://doi.org/10.1016/j.jpba.2016.06.043 .,
Kon_3215 .
1
3
1
2

Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity

Takić, Marija M.; Jovanović, Vesna B.; Pavićević, Ivan D.; Uzelac, Tamara N.; Aćimović, Jelena M.; Ristić-Medić, Danijela; Mandić, Ljuba M.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Takić, Marija M.
AU  - Jovanović, Vesna B.
AU  - Pavićević, Ivan D.
AU  - Uzelac, Tamara N.
AU  - Aćimović, Jelena M.
AU  - Ristić-Medić, Danijela
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2054
AB  - The interaction of polyphenolic molecules with human serum albumin (HSA) could lead to changes in the reactivity of the HSA Cys34 thiol group (HSA-SH). The influences of enterolactone (EL) and enterodiol (ED) binding on HSA-SH reactivity in fatty acid (FA)-free HSA, and in HSA with bound stearic acid (S) in S/HSA molar ratios of 1 : 1 and 4 : 1, were investigated by the determination of the pseudo first order rate constants (k') for the thiol reaction with 5,5'-dithiobis-(2-nitrobenzoic acid). The binding affinities and binding sites of EL and ED were also determined, using fluorescence measurements of the intrinsic fluorescence of Trp214 and diazepam (binding site marker). EL and ED binding to HSA increased the reactivity of HSA-SH in all assayed HSA-enterolignan complexes by 9.1-33.1%. The strongest effects were obtained for FA-free HSA-enterolignan complexes. S modulated/reduced the effect of EL on HSA-SH reactivity, while its influence on the effect of ED was negligible. The binding of enterolignans to HSA was investigated: the binding constants were the highest for FA-free HSA (EL: 11.64 x 10(4) M-1 and ED: 5.59 x 10(4) M-1 at 37 degrees C) and the lowest for S/HSA 4 : 1-enterolignan complexes (EL: 2.43 x 10(4) M-1 and ED: 1.92 x 10(4) M-1). When the S/HSA ratio was increased, the binding affinities and number of binding sites for EL and ED were decreased. At the same time, a high correlation between binding constants and increased Cys34 reactivity was found (r = 0.974). Competitive experiments using diazepam indicated that the binding of ED and of EL was located in the hydrophobic pocket of site II in HSA. Overall, it is evident that stearic acid could modulate the enterolignan effects on HSA-SH reactivity as well as their binding to HSA. This finding could be important for pharmacokinetics and the expression of enterolignan antioxidant effects in vivo after an intake of lignan rich food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food and Function
T1  - Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity
VL  - 7
IS  - 2
SP  - 1217
EP  - 1226
DO  - 10.1039/c5fo01346a
UR  - Kon_3010
ER  - 
@article{
author = "Takić, Marija M. and Jovanović, Vesna B. and Pavićević, Ivan D. and Uzelac, Tamara N. and Aćimović, Jelena M. and Ristić-Medić, Danijela and Mandić, Ljuba M.",
year = "2016",
abstract = "The interaction of polyphenolic molecules with human serum albumin (HSA) could lead to changes in the reactivity of the HSA Cys34 thiol group (HSA-SH). The influences of enterolactone (EL) and enterodiol (ED) binding on HSA-SH reactivity in fatty acid (FA)-free HSA, and in HSA with bound stearic acid (S) in S/HSA molar ratios of 1 : 1 and 4 : 1, were investigated by the determination of the pseudo first order rate constants (k') for the thiol reaction with 5,5'-dithiobis-(2-nitrobenzoic acid). The binding affinities and binding sites of EL and ED were also determined, using fluorescence measurements of the intrinsic fluorescence of Trp214 and diazepam (binding site marker). EL and ED binding to HSA increased the reactivity of HSA-SH in all assayed HSA-enterolignan complexes by 9.1-33.1%. The strongest effects were obtained for FA-free HSA-enterolignan complexes. S modulated/reduced the effect of EL on HSA-SH reactivity, while its influence on the effect of ED was negligible. The binding of enterolignans to HSA was investigated: the binding constants were the highest for FA-free HSA (EL: 11.64 x 10(4) M-1 and ED: 5.59 x 10(4) M-1 at 37 degrees C) and the lowest for S/HSA 4 : 1-enterolignan complexes (EL: 2.43 x 10(4) M-1 and ED: 1.92 x 10(4) M-1). When the S/HSA ratio was increased, the binding affinities and number of binding sites for EL and ED were decreased. At the same time, a high correlation between binding constants and increased Cys34 reactivity was found (r = 0.974). Competitive experiments using diazepam indicated that the binding of ED and of EL was located in the hydrophobic pocket of site II in HSA. Overall, it is evident that stearic acid could modulate the enterolignan effects on HSA-SH reactivity as well as their binding to HSA. This finding could be important for pharmacokinetics and the expression of enterolignan antioxidant effects in vivo after an intake of lignan rich food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food and Function",
title = "Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity",
volume = "7",
number = "2",
pages = "1217-1226",
doi = "10.1039/c5fo01346a",
url = "Kon_3010"
}
Takić, M. M., Jovanović, V. B., Pavićević, I. D., Uzelac, T. N., Aćimović, J. M., Ristić-Medić, D.,& Mandić, L. M.. (2016). Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity. in Food and Function
Royal Soc Chemistry, Cambridge., 7(2), 1217-1226.
https://doi.org/10.1039/c5fo01346a
Kon_3010
Takić MM, Jovanović VB, Pavićević ID, Uzelac TN, Aćimović JM, Ristić-Medić D, Mandić LM. Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity. in Food and Function. 2016;7(2):1217-1226.
doi:10.1039/c5fo01346a
Kon_3010 .
Takić, Marija M., Jovanović, Vesna B., Pavićević, Ivan D., Uzelac, Tamara N., Aćimović, Jelena M., Ristić-Medić, Danijela, Mandić, Ljuba M., "Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity" in Food and Function, 7, no. 2 (2016):1217-1226,
https://doi.org/10.1039/c5fo01346a .,
Kon_3010 .
1
11
11
10

Translation of P = kT into a pictorial external representation by high school seniors

Matijasevic, Igor; Korolija, Jasminka N.; Mandić, Ljuba M.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Matijasevic, Igor
AU  - Korolija, Jasminka N.
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2339
AB  - This paper describes the results achieved by high school seniors on an item which involves translation of the equation P = kT into a corresponding pictorial external representation. The majority of students (the classes of 2011, 2012 and 2013) did not give the correct answer to the multiple choice part of the translation item. They chose pictorial representations of the other gas laws (P = k/V, or V = kT) instead. Failure to choose the correct answer was surprising considering that the symbol for volume was absent which should have been the key clue. Through the analysis of students' explanations (the classes of 2011 and 2012) and interviews (the class of 2013) we considered the reasoning applied by students who chose the correct answer or distractors for the multiple choice part of the item. Among the students who answered correctly there were explanations which contained misconceptions. Several factors that lead to the unsuccessful translation between external representations have been discovered. Students interpreted the change in one quantity based on the notation for the change in another one because of deep rooted cognitive schemas about changing two quantities (volume and pressure, pressure and temperature, temperature and volume), without consideration that for such changes to be valid for gases all three quantities need to be considered for a certain amount of substance. Those cognitive schemas interfered with mathematical reasoning, i.e. students possessed limited understanding of the equations.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemistry Education: Research and Practice
T1  - Translation of P = kT into a pictorial external representation by high school seniors
VL  - 17
IS  - 4
SP  - 656
EP  - 674
DO  - 10.1039/c6rp00030d
UR  - Kon_3155
ER  - 
@article{
author = "Matijasevic, Igor and Korolija, Jasminka N. and Mandić, Ljuba M.",
year = "2016",
abstract = "This paper describes the results achieved by high school seniors on an item which involves translation of the equation P = kT into a corresponding pictorial external representation. The majority of students (the classes of 2011, 2012 and 2013) did not give the correct answer to the multiple choice part of the translation item. They chose pictorial representations of the other gas laws (P = k/V, or V = kT) instead. Failure to choose the correct answer was surprising considering that the symbol for volume was absent which should have been the key clue. Through the analysis of students' explanations (the classes of 2011 and 2012) and interviews (the class of 2013) we considered the reasoning applied by students who chose the correct answer or distractors for the multiple choice part of the item. Among the students who answered correctly there were explanations which contained misconceptions. Several factors that lead to the unsuccessful translation between external representations have been discovered. Students interpreted the change in one quantity based on the notation for the change in another one because of deep rooted cognitive schemas about changing two quantities (volume and pressure, pressure and temperature, temperature and volume), without consideration that for such changes to be valid for gases all three quantities need to be considered for a certain amount of substance. Those cognitive schemas interfered with mathematical reasoning, i.e. students possessed limited understanding of the equations.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemistry Education: Research and Practice",
title = "Translation of P = kT into a pictorial external representation by high school seniors",
volume = "17",
number = "4",
pages = "656-674",
doi = "10.1039/c6rp00030d",
url = "Kon_3155"
}
Matijasevic, I., Korolija, J. N.,& Mandić, L. M.. (2016). Translation of P = kT into a pictorial external representation by high school seniors. in Chemistry Education: Research and Practice
Royal Soc Chemistry, Cambridge., 17(4), 656-674.
https://doi.org/10.1039/c6rp00030d
Kon_3155
Matijasevic I, Korolija JN, Mandić LM. Translation of P = kT into a pictorial external representation by high school seniors. in Chemistry Education: Research and Practice. 2016;17(4):656-674.
doi:10.1039/c6rp00030d
Kon_3155 .
Matijasevic, Igor, Korolija, Jasminka N., Mandić, Ljuba M., "Translation of P = kT into a pictorial external representation by high school seniors" in Chemistry Education: Research and Practice, 17, no. 4 (2016):656-674,
https://doi.org/10.1039/c6rp00030d .,
Kon_3155 .
1
5
4
4

Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model

Đekić-Ivanković, Marija; Weiler, Hope A.; Nikolic, Marina; Kadvan, Agnes; Gurinovic, Mirjana; Mandić, Ljuba M.; Glibetic, Maria

(Cambridge Univ Press, Cambridge, 2016)

TY  - JOUR
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope A.
AU  - Nikolic, Marina
AU  - Kadvan, Agnes
AU  - Gurinovic, Mirjana
AU  - Mandić, Ljuba M.
AU  - Glibetic, Maria
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1905
AB  - Objective: The objective of the present study was to examine the external validity of an FFQ designed to estimate dietary vitamin D intake compared with a plasma biomarker and three repeated 24 h dietary recalls in women of reproductive age in Serbia, where there is no exposure to food fortified with vitamin D. The method of triads was applied. Design: In a cross-sectional study, 422 women completed the Women and Reproductive Health FFQ (WRH-FFQ) during the winter months. From a representative subgroup (n 44), three 24 h dietary recalls and anthropometric parameters were collected as well as a fasting blood sample for vitamin D biomarker analyses. Correlation coefficients were calculated between each of the dietary methods. Validity coefficients, as a correlation between the measured and estimated 'true' exposure, were calculated using the method of triads. Bland-Altman plots were also constructed. Setting: Three major universities in Serbia. Subjects: Healthy young women (n 422) aged 18-35 years. Results: The WRH-FFQ estimate of vitamin D intake for all participants was 4.0 (SD 3.3) mu g/d and 3.1 (SD 2.3) mu g/d for the subgroup. Bland-Altman plots for these intakes showed high agreement. Validity coefficients for the FFQ, 24 h recall and biomarker were. rho(QI) = 0.847 (95 % CI 0.564, 0.928), rho(RI) = 0.810 (95 % CI 0.537, 0.997) and rho(BI) = 0.499 (95 % CI 0.190, 0.840), while the correlation coefficients were 0.686, 0.422 and 0.404. Conclusions: The FFQ applied in the present study is a valid tool for assessing dietary vitamin D intake in women living in Serbia, a region without mandatory vitamin D food fortification.
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model
VL  - 19
IS  - 4
SP  - 437
EP  - 445
DO  - 10.1017/S136898001500138X
UR  - Kon_3024
ER  - 
@article{
author = "Đekić-Ivanković, Marija and Weiler, Hope A. and Nikolic, Marina and Kadvan, Agnes and Gurinovic, Mirjana and Mandić, Ljuba M. and Glibetic, Maria",
year = "2016",
abstract = "Objective: The objective of the present study was to examine the external validity of an FFQ designed to estimate dietary vitamin D intake compared with a plasma biomarker and three repeated 24 h dietary recalls in women of reproductive age in Serbia, where there is no exposure to food fortified with vitamin D. The method of triads was applied. Design: In a cross-sectional study, 422 women completed the Women and Reproductive Health FFQ (WRH-FFQ) during the winter months. From a representative subgroup (n 44), three 24 h dietary recalls and anthropometric parameters were collected as well as a fasting blood sample for vitamin D biomarker analyses. Correlation coefficients were calculated between each of the dietary methods. Validity coefficients, as a correlation between the measured and estimated 'true' exposure, were calculated using the method of triads. Bland-Altman plots were also constructed. Setting: Three major universities in Serbia. Subjects: Healthy young women (n 422) aged 18-35 years. Results: The WRH-FFQ estimate of vitamin D intake for all participants was 4.0 (SD 3.3) mu g/d and 3.1 (SD 2.3) mu g/d for the subgroup. Bland-Altman plots for these intakes showed high agreement. Validity coefficients for the FFQ, 24 h recall and biomarker were. rho(QI) = 0.847 (95 % CI 0.564, 0.928), rho(RI) = 0.810 (95 % CI 0.537, 0.997) and rho(BI) = 0.499 (95 % CI 0.190, 0.840), while the correlation coefficients were 0.686, 0.422 and 0.404. Conclusions: The FFQ applied in the present study is a valid tool for assessing dietary vitamin D intake in women living in Serbia, a region without mandatory vitamin D food fortification.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model",
volume = "19",
number = "4",
pages = "437-445",
doi = "10.1017/S136898001500138X",
url = "Kon_3024"
}
Đekić-Ivanković, M., Weiler, H. A., Nikolic, M., Kadvan, A., Gurinovic, M., Mandić, L. M.,& Glibetic, M.. (2016). Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model. in Public Health Nutrition
Cambridge Univ Press, Cambridge., 19(4), 437-445.
https://doi.org/10.1017/S136898001500138X
Kon_3024
Đekić-Ivanković M, Weiler HA, Nikolic M, Kadvan A, Gurinovic M, Mandić LM, Glibetic M. Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model. in Public Health Nutrition. 2016;19(4):437-445.
doi:10.1017/S136898001500138X
Kon_3024 .
Đekić-Ivanković, Marija, Weiler, Hope A., Nikolic, Marina, Kadvan, Agnes, Gurinovic, Mirjana, Mandić, Ljuba M., Glibetic, Maria, "Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model" in Public Health Nutrition, 19, no. 4 (2016):437-445,
https://doi.org/10.1017/S136898001500138X .,
Kon_3024 .
21
14
16

Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X

Đekić-Ivanković, Marija; Weiler, Hope A.; Nikolic, Marina; Kadvan, Agnes; Gurinovic, Mirjana; Mandić, Ljuba M.; Glibetic, Maria

(Cambridge Univ Press, Cambridge, 2016)

TY  - DATA
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope A.
AU  - Nikolic, Marina
AU  - Kadvan, Agnes
AU  - Gurinovic, Mirjana
AU  - Mandić, Ljuba M.
AU  - Glibetic, Maria
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3658
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3658
ER  - 
@misc{
author = "Đekić-Ivanković, Marija and Weiler, Hope A. and Nikolic, Marina and Kadvan, Agnes and Gurinovic, Mirjana and Mandić, Ljuba M. and Glibetic, Maria",
year = "2016",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3658"
}
Đekić-Ivanković, M., Weiler, H. A., Nikolic, M., Kadvan, A., Gurinovic, M., Mandić, L. M.,& Glibetic, M.. (2016). Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X. in Public Health Nutrition
Cambridge Univ Press, Cambridge..
https://hdl.handle.net/21.15107/rcub_cherry_3658
Đekić-Ivanković M, Weiler HA, Nikolic M, Kadvan A, Gurinovic M, Mandić LM, Glibetic M. Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X. in Public Health Nutrition. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3658 .
Đekić-Ivanković, Marija, Weiler, Hope A., Nikolic, Marina, Kadvan, Agnes, Gurinovic, Mirjana, Mandić, Ljuba M., Glibetic, Maria, "Supplementary data for the article: Djekic-Ivankovic, M.; Weiler, H. A.; Nikolic, M.; Kadvan, A.; Gurinovic, M.; Mandic, L. M.; Glibetic, M. Validity of an FFQ Assessing the Vitamin D Intake of Young Serbian Women Living in a Region without Food Fortification: The Method of Triads Model. Public Health Nutr. 2016, 19 (4), 437–445. https://doi.org/10.1017/S136898001500138X" in Public Health Nutrition (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3658 .

HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions

Penezić, Ana Z.; Jovanović, Vesna B.; Pavićević, Ivan D.; Aćimović, Jelena M.; Mandić, Ljuba M.

(Royal Soc Chemistry, Cambridge, 2015)

TY  - JOUR
AU  - Penezić, Ana Z.
AU  - Jovanović, Vesna B.
AU  - Pavićević, Ivan D.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1979
AB  - The potential of carbonylation with methylglyoxal to alter HSA's binding affinity for copper(II) ions and its influence on the release of copper(II) ions from copper-HSA complexes were studied. The affinity of HSA to coordinate copper(II) decreased upon carbonylation of the Cys34-SH group. Carbonylation of copper-HSA complexes caused a decrease in Cys34-SH content, conformational changes and the release of copper(II) ions. The ratio between the percentage of reduction in the Cys34-SH group content and the percentage of release of copper(II) from complexes is 2.12 +/- 0.28. Because the same ratio (1.96 +/- 0.36) was obtained upon oxidation of the Cys34-SH group (with no changes in HSA conformation), the binding/release of copper (II) by HSA depended mainly on the redox state of the Cys34-SH group. The contents of Cys34-SH and HSA-bound copper(II) ions in the diabetic group (0.457 +/- 0.081 mol SH per mol HSA, 10.7 +/- 0.01 mmol per mol HSA, resp.) were significantly lower (p  lt  0.01) compared to the control group (0.609 +/- 0.027 mol SH per mol HSA; 13.4 +/- 0.01 mmol per mol HSA, resp.). Very strong correlations between the values for HSA-SH and glycated haemoglobin, HbA1c, (R = -0.803, p  lt  0.01), and between the values for the HSA-bound copper(II) content and HSA-SH content (R = 0.841, p  lt  0.002) were found in the diabetic group. Thus, HSA carbonylation leads to decrease in HSA-SH content and to the impairment of its copper(II) binding capacity that could contribute to further enhancement of oxidative and carbonyl stress in diabetes (as well as in other diseases with carbonyl stress).
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions
VL  - 7
IS  - 10
SP  - 1431
EP  - 1438
DO  - 10.1039/c5mt00159e
UR  - Kon_2934
ER  - 
@article{
author = "Penezić, Ana Z. and Jovanović, Vesna B. and Pavićević, Ivan D. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2015",
abstract = "The potential of carbonylation with methylglyoxal to alter HSA's binding affinity for copper(II) ions and its influence on the release of copper(II) ions from copper-HSA complexes were studied. The affinity of HSA to coordinate copper(II) decreased upon carbonylation of the Cys34-SH group. Carbonylation of copper-HSA complexes caused a decrease in Cys34-SH content, conformational changes and the release of copper(II) ions. The ratio between the percentage of reduction in the Cys34-SH group content and the percentage of release of copper(II) from complexes is 2.12 +/- 0.28. Because the same ratio (1.96 +/- 0.36) was obtained upon oxidation of the Cys34-SH group (with no changes in HSA conformation), the binding/release of copper (II) by HSA depended mainly on the redox state of the Cys34-SH group. The contents of Cys34-SH and HSA-bound copper(II) ions in the diabetic group (0.457 +/- 0.081 mol SH per mol HSA, 10.7 +/- 0.01 mmol per mol HSA, resp.) were significantly lower (p  lt  0.01) compared to the control group (0.609 +/- 0.027 mol SH per mol HSA; 13.4 +/- 0.01 mmol per mol HSA, resp.). Very strong correlations between the values for HSA-SH and glycated haemoglobin, HbA1c, (R = -0.803, p  lt  0.01), and between the values for the HSA-bound copper(II) content and HSA-SH content (R = 0.841, p  lt  0.002) were found in the diabetic group. Thus, HSA carbonylation leads to decrease in HSA-SH content and to the impairment of its copper(II) binding capacity that could contribute to further enhancement of oxidative and carbonyl stress in diabetes (as well as in other diseases with carbonyl stress).",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions",
volume = "7",
number = "10",
pages = "1431-1438",
doi = "10.1039/c5mt00159e",
url = "Kon_2934"
}
Penezić, A. Z., Jovanović, V. B., Pavićević, I. D., Aćimović, J. M.,& Mandić, L. M.. (2015). HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions. in Metallomics
Royal Soc Chemistry, Cambridge., 7(10), 1431-1438.
https://doi.org/10.1039/c5mt00159e
Kon_2934
Penezić AZ, Jovanović VB, Pavićević ID, Aćimović JM, Mandić LM. HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions. in Metallomics. 2015;7(10):1431-1438.
doi:10.1039/c5mt00159e
Kon_2934 .
Penezić, Ana Z., Jovanović, Vesna B., Pavićević, Ivan D., Aćimović, Jelena M., Mandić, Ljuba M., "HSA carbonylation with methylglyoxal and the binding/release of copper(II) ions" in Metallomics, 7, no. 10 (2015):1431-1438,
https://doi.org/10.1039/c5mt00159e .,
Kon_2934 .
7
6
7

"It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education

Korolija, Jasminka N.; Rajic, Snezana; Tosic, Milena; Mandić, Ljuba M.

(Serbian Chemical Soc, Belgrade, 2015)

TY  - JOUR
AU  - Korolija, Jasminka N.
AU  - Rajic, Snezana
AU  - Tosic, Milena
AU  - Mandić, Ljuba M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2016
AB  - In order to improve the ability to apply knowledge of chemistry (acquired in the existing educational system) in real life, the model for consideration of ecological issues was developed and applied in high school. The model consists of a continuous text "It Happened, What's the Problem?" and a test with non-continuous text "A Guide Through the Problem", which were prepared for consideration of the problem of eutrophication. All results obtained (average achievement of 70.9 +/- 14.3 %) showed that the application of the model enabled: understanding of an ecological problem based on scientific representations of the term eutrophication given in the continuous text, realization that pollution of the environment may be directly related to modern life, application of acquired knowledge of chemistry to observe and understand the cause and effect of eutrophication in the environment, to draw a scientific conclusion, and understanding the importance of science and technology discoveries for solving ecological problems. In addition, the model contributed to the development of student's environmental literacy (ecological knowledge and cognitive skills), ability to think critically, and provided possibilities for classroom knowledge to become applicable in real life.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - "It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education
VL  - 80
IS  - 12
SP  - 1567
EP  - 1580
DO  - 10.2298/JSC150522072K
UR  - Kon_2971
ER  - 
@article{
author = "Korolija, Jasminka N. and Rajic, Snezana and Tosic, Milena and Mandić, Ljuba M.",
year = "2015",
abstract = "In order to improve the ability to apply knowledge of chemistry (acquired in the existing educational system) in real life, the model for consideration of ecological issues was developed and applied in high school. The model consists of a continuous text "It Happened, What's the Problem?" and a test with non-continuous text "A Guide Through the Problem", which were prepared for consideration of the problem of eutrophication. All results obtained (average achievement of 70.9 +/- 14.3 %) showed that the application of the model enabled: understanding of an ecological problem based on scientific representations of the term eutrophication given in the continuous text, realization that pollution of the environment may be directly related to modern life, application of acquired knowledge of chemistry to observe and understand the cause and effect of eutrophication in the environment, to draw a scientific conclusion, and understanding the importance of science and technology discoveries for solving ecological problems. In addition, the model contributed to the development of student's environmental literacy (ecological knowledge and cognitive skills), ability to think critically, and provided possibilities for classroom knowledge to become applicable in real life.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = ""It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education",
volume = "80",
number = "12",
pages = "1567-1580",
doi = "10.2298/JSC150522072K",
url = "Kon_2971"
}
Korolija, J. N., Rajic, S., Tosic, M.,& Mandić, L. M.. (2015). "It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(12), 1567-1580.
https://doi.org/10.2298/JSC150522072K
Kon_2971
Korolija JN, Rajic S, Tosic M, Mandić LM. "It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education. in Journal of the Serbian Chemical Society. 2015;80(12):1567-1580.
doi:10.2298/JSC150522072K
Kon_2971 .
Korolija, Jasminka N., Rajic, Snezana, Tosic, Milena, Mandić, Ljuba M., ""It happened, what's the problem?" and "A guide through the problem" - A model for consideration of ecological issues in chemistry education" in Journal of the Serbian Chemical Society, 80, no. 12 (2015):1567-1580,
https://doi.org/10.2298/JSC150522072K .,
Kon_2971 .
1
1
1

Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia

Đekić-Ivanković, Marija; Weiler, Hope; Jones, Glenville; Kaufmann, Martin; Kaluđerović, Jovana; Aleksić-Veličković, Vesna; Mandić, Ljuba M.; Glibetic, Maria

(Federation Amer Soc Exp Biol, Bethesda, 2015)

TY  - CONF
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Jones, Glenville
AU  - Kaufmann, Martin
AU  - Kaluđerović, Jovana
AU  - Aleksić-Veličković, Vesna
AU  - Mandić, Ljuba M.
AU  - Glibetic, Maria
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1969
PB  - Federation Amer Soc Exp Biol, Bethesda
C3  - FASEB Journal / Federation of American Societies for Experimental Biology
T1  - Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia
VL  - 29
UR  - Kon_2924
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1969
ER  - 
@conference{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Jones, Glenville and Kaufmann, Martin and Kaluđerović, Jovana and Aleksić-Veličković, Vesna and Mandić, Ljuba M. and Glibetic, Maria",
year = "2015",
publisher = "Federation Amer Soc Exp Biol, Bethesda",
journal = "FASEB Journal / Federation of American Societies for Experimental Biology",
title = "Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia",
volume = "29",
url = "Kon_2924, https://hdl.handle.net/21.15107/rcub_cherry_1969"
}
Đekić-Ivanković, M., Weiler, H., Jones, G., Kaufmann, M., Kaluđerović, J., Aleksić-Veličković, V., Mandić, L. M.,& Glibetic, M.. (2015). Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia. in FASEB Journal / Federation of American Societies for Experimental Biology
Federation Amer Soc Exp Biol, Bethesda., 29.
Kon_2924
Đekić-Ivanković M, Weiler H, Jones G, Kaufmann M, Kaluđerović J, Aleksić-Veličković V, Mandić LM, Glibetic M. Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia. in FASEB Journal / Federation of American Societies for Experimental Biology. 2015;29.
Kon_2924 .
Đekić-Ivanković, Marija, Weiler, Hope, Jones, Glenville, Kaufmann, Martin, Kaluđerović, Jovana, Aleksić-Veličković, Vesna, Mandić, Ljuba M., Glibetic, Maria, "Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia" in FASEB Journal / Federation of American Societies for Experimental Biology, 29 (2015),
Kon_2924 .

The influence of fatty acids on determination of human serum albumin thiol group

Jovanović, Vesna B.; Pavićević, Ivan D.; Takić, Marija M.; Penezić-Romanjuk, Ana Z.; Aćimović, Jelena M.; Mandić, Ljuba M.

(Academic Press Inc Elsevier Science, San Diego, 2014)

TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Pavićević, Ivan D.
AU  - Takić, Marija M.
AU  - Penezić-Romanjuk, Ana Z.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1476
AB  - During investigation of the changes of the Cys34 thiol group of human serum albumin (HSA) (isolated by affinity chromatography with Cibacron Blue (CB)) in diabetes, we found that the HSA-SH content was higher (11-33%) than the total serum thiol content. The influence of fatty acids (FA) binding to HSA on this discrepancy was investigated in vitro (using fluorescence and CD spectroscopy and GC) and with HSA samples from diabetic (n=20) and control groups (n=17). HSA-bound FA determine the selection of HSA molecules by CB and enhance reactivity and/or accessibility of the SH group. A high content of polyunsaturated FA (35.6%) leads to weaker binding of HSA molecules to CB. Rate constants of DTNB reaction with the SH group of HSA applied to a CB column, bound-HSA and unbound-HSA fractions, were 4.8 x 10(-3), 21.6 x 10(-3), and 11.2 x 10(-3) s(-1), respectively. The HSA-SH group of diabetics is more reactive compared with control individuals (rate constants 20.9 x 10(-3)+/- 4.4 x 10(-3) vs 12.9 x 10(-3)+/- 2.6 x 10(-3) s(-1), P lt 0.05). Recovery values of the SH group obtained after chromatography of HSA with bound stearic acid ranged from 110 to 140%, while those for defatted HSA were from 98.5 to 101.7%. Thus, HSA-bound FA leads to an increase of HSA-SH content and a contribution to total serum thiols, which make the determination of the thiol group unreliable. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - The influence of fatty acids on determination of human serum albumin thiol group
VL  - 448
SP  - 50
EP  - 57
DO  - 10.1016/j.ab.2013.11.030
UR  - Kon_2596
ER  - 
@article{
author = "Jovanović, Vesna B. and Pavićević, Ivan D. and Takić, Marija M. and Penezić-Romanjuk, Ana Z. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2014",
abstract = "During investigation of the changes of the Cys34 thiol group of human serum albumin (HSA) (isolated by affinity chromatography with Cibacron Blue (CB)) in diabetes, we found that the HSA-SH content was higher (11-33%) than the total serum thiol content. The influence of fatty acids (FA) binding to HSA on this discrepancy was investigated in vitro (using fluorescence and CD spectroscopy and GC) and with HSA samples from diabetic (n=20) and control groups (n=17). HSA-bound FA determine the selection of HSA molecules by CB and enhance reactivity and/or accessibility of the SH group. A high content of polyunsaturated FA (35.6%) leads to weaker binding of HSA molecules to CB. Rate constants of DTNB reaction with the SH group of HSA applied to a CB column, bound-HSA and unbound-HSA fractions, were 4.8 x 10(-3), 21.6 x 10(-3), and 11.2 x 10(-3) s(-1), respectively. The HSA-SH group of diabetics is more reactive compared with control individuals (rate constants 20.9 x 10(-3)+/- 4.4 x 10(-3) vs 12.9 x 10(-3)+/- 2.6 x 10(-3) s(-1), P lt 0.05). Recovery values of the SH group obtained after chromatography of HSA with bound stearic acid ranged from 110 to 140%, while those for defatted HSA were from 98.5 to 101.7%. Thus, HSA-bound FA leads to an increase of HSA-SH content and a contribution to total serum thiols, which make the determination of the thiol group unreliable. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "The influence of fatty acids on determination of human serum albumin thiol group",
volume = "448",
pages = "50-57",
doi = "10.1016/j.ab.2013.11.030",
url = "Kon_2596"
}
Jovanović, V. B., Pavićević, I. D., Takić, M. M., Penezić-Romanjuk, A. Z., Aćimović, J. M.,& Mandić, L. M.. (2014). The influence of fatty acids on determination of human serum albumin thiol group. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 448, 50-57.
https://doi.org/10.1016/j.ab.2013.11.030
Kon_2596
Jovanović VB, Pavićević ID, Takić MM, Penezić-Romanjuk AZ, Aćimović JM, Mandić LM. The influence of fatty acids on determination of human serum albumin thiol group. in Analytical Biochemistry. 2014;448:50-57.
doi:10.1016/j.ab.2013.11.030
Kon_2596 .
Jovanović, Vesna B., Pavićević, Ivan D., Takić, Marija M., Penezić-Romanjuk, Ana Z., Aćimović, Jelena M., Mandić, Ljuba M., "The influence of fatty acids on determination of human serum albumin thiol group" in Analytical Biochemistry, 448 (2014):50-57,
https://doi.org/10.1016/j.ab.2013.11.030 .,
Kon_2596 .
12
11
11

The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal

Aćimović, Jelena M.; Penezić, Ana Z.; Pavićević, Ivan D.; Jovanović, Vesna B.; Mandić, Ljuba M.

(Royal Soc Chemistry, Cambridge, 2014)

TY  - JOUR
AU  - Aćimović, Jelena M.
AU  - Penezić, Ana Z.
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1814
AB  - alpha-Oxoaldehydes, which are produced in higher quantities in diabetes, uremia, oxidative stress, inflammation and aging, react with the amino, guanidine and thiol groups of proteins and cause the formation of advanced glycated end-products and protein cross-linking. To prevent these reactions, the efficiency of tow molecular mass thiols with an alpha-amino-beta-mercapto-ethane group (Cys, penicillamine and N-acetylcysteine (NAcCys, with a blocked amino group)) as scavengers of methylglyoxal, compared with glutathione (GSH) and the biguanidine derivative metformin, was investigated. The time courses of the reactions of the aforementioned compounds with methylglyoxal were assayed. The reactivity of their thiol and amino groups decreased in the order of Cys  gt  penicillamine  gt  GSH  gt  NAcCys and penicillamine  gt  Cys  gt  GSH, respectively. Human serum albumin (HSA) carbonylation in the absence or presence of methylglyoxal scavengers were monitored by the determination of the amino, guanidine and thiol groups' contents, as well as by spectrofluorimetry, CD and native and SDS PAGE. Cys and penicillamine were highly efficient in the prevention of the carbonylation of the HSA-amino (for 80%) and guanidine (for 84% and 55%, respectively) groups and the formation of fluorescent AGEs. GSH and metformin exhibited medium efficiency (reduction of amino group's carbonylation for 60% and guanidine for about 30%); the least efficient was NAcCys. The presence of Cys, penicillamine and NAcCys led to an almost complete protection of the HSA-thiol group's carbonylation, whereas metformin was inefficient. The efficiency in the prevention of protein cross-linking increased in the order of metformin, NAcCys  lt  GSH  lt  penicillamine  lt  Cys. Thus, the substances with an alpha-amino-beta-mercapto-ethane group as a pharmacophore exhibit great potential as an efficient methylglyoxal scavengers, and are thus promising compounds for medicinal chemistry. In addition, they protect the HSA-SH group and preserve its antioxidative potential, which is very important for the HSA's function in vivo.
PB  - Royal Soc Chemistry, Cambridge
T2  - Molecular BioSystems
T1  - The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal
VL  - 10
IS  - 8
SP  - 2166
EP  - 2175
DO  - 10.1039/c4mb00217b
UR  - Kon_2697
ER  - 
@article{
author = "Aćimović, Jelena M. and Penezić, Ana Z. and Pavićević, Ivan D. and Jovanović, Vesna B. and Mandić, Ljuba M.",
year = "2014",
abstract = "alpha-Oxoaldehydes, which are produced in higher quantities in diabetes, uremia, oxidative stress, inflammation and aging, react with the amino, guanidine and thiol groups of proteins and cause the formation of advanced glycated end-products and protein cross-linking. To prevent these reactions, the efficiency of tow molecular mass thiols with an alpha-amino-beta-mercapto-ethane group (Cys, penicillamine and N-acetylcysteine (NAcCys, with a blocked amino group)) as scavengers of methylglyoxal, compared with glutathione (GSH) and the biguanidine derivative metformin, was investigated. The time courses of the reactions of the aforementioned compounds with methylglyoxal were assayed. The reactivity of their thiol and amino groups decreased in the order of Cys  gt  penicillamine  gt  GSH  gt  NAcCys and penicillamine  gt  Cys  gt  GSH, respectively. Human serum albumin (HSA) carbonylation in the absence or presence of methylglyoxal scavengers were monitored by the determination of the amino, guanidine and thiol groups' contents, as well as by spectrofluorimetry, CD and native and SDS PAGE. Cys and penicillamine were highly efficient in the prevention of the carbonylation of the HSA-amino (for 80%) and guanidine (for 84% and 55%, respectively) groups and the formation of fluorescent AGEs. GSH and metformin exhibited medium efficiency (reduction of amino group's carbonylation for 60% and guanidine for about 30%); the least efficient was NAcCys. The presence of Cys, penicillamine and NAcCys led to an almost complete protection of the HSA-thiol group's carbonylation, whereas metformin was inefficient. The efficiency in the prevention of protein cross-linking increased in the order of metformin, NAcCys  lt  GSH  lt  penicillamine  lt  Cys. Thus, the substances with an alpha-amino-beta-mercapto-ethane group as a pharmacophore exhibit great potential as an efficient methylglyoxal scavengers, and are thus promising compounds for medicinal chemistry. In addition, they protect the HSA-SH group and preserve its antioxidative potential, which is very important for the HSA's function in vivo.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Molecular BioSystems",
title = "The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal",
volume = "10",
number = "8",
pages = "2166-2175",
doi = "10.1039/c4mb00217b",
url = "Kon_2697"
}
Aćimović, J. M., Penezić, A. Z., Pavićević, I. D., Jovanović, V. B.,& Mandić, L. M.. (2014). The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal. in Molecular BioSystems
Royal Soc Chemistry, Cambridge., 10(8), 2166-2175.
https://doi.org/10.1039/c4mb00217b
Kon_2697
Aćimović JM, Penezić AZ, Pavićević ID, Jovanović VB, Mandić LM. The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal. in Molecular BioSystems. 2014;10(8):2166-2175.
doi:10.1039/c4mb00217b
Kon_2697 .
Aćimović, Jelena M., Penezić, Ana Z., Pavićević, Ivan D., Jovanović, Vesna B., Mandić, Ljuba M., "The efficiency of compounds with alpha-amino-beta-mercapto-ethane group in protection of human serum albumin carbonylation and cross-linking with methylglyoxal" in Molecular BioSystems, 10, no. 8 (2014):2166-2175,
https://doi.org/10.1039/c4mb00217b .,
Kon_2697 .
7
6
6

Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal

Pavićević, Ivan D.; Jovanović, Vesna B.; Takić, Marija M.; Penezić, Ana Z.; Aćimović, Jelena M.; Mandić, Ljuba M.

(Elsevier Ireland Ltd, Clare, 2014)

TY  - JOUR
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija M.
AU  - Penezić, Ana Z.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1895
AB  - Fatty acids (FAs) binding to human serum albumin (HSA) could lead to the changes of Cys-34 thiol group accessibility and reactivity, i.e. its scavenger capacity and antioxidant property. The influence of saturated, mono and poly unsaturated, and fish oil FAs binding to HSA on the carbonylation level and the reactivity of HSA-SH and HSA modified with methylglyoxal (MG-HSA-SH) was investigated. Changes of thiol group reactivity were followed by determination of pseudo first order rate constant (k') for thiols reaction with 5,5'-dithiobis(2-nitrobenzoic acid). HSA changes were monitored using native PAG electrophoresis and fluorescence spectroscopy. For FA/HSA molar ratios screening, qTLC and GC were used. FAs increase thiol group carbonylation levels from 8% to 20%. The k' values obtained for FAs-free HSA-SH and FAs-free MG-HSA-SH are almost equal (7.5 x 10(-3) and 7.7 x 10(-3) resp.). Binding of all FAs amplify the reactivity (k' values from 14.6 x 10(-3) to 26.0 x 10(-3) s(-1)) of HSA-SH group for 2-3.5 times in the order: palmitic, docosahexaenoic, fish oil extract, stearic, oleic, myristic and eicosapentaenoic acid, due to HSA conformational changes. FAs-bound MG-HSA-SH samples follow that pattern, but their k' values (from 9.8 x 10(-3) to 14.3 x 10(-3) s(-1)) were lower compared to unmodified HSA due to additional conformation changes of HSA molecules during carbonylation. Carbonylation level and reactivity of Cys34 thiol group of unmodified and carbonylated HSA depend on type of FAs bound to HSA, which implies the possibility for modulation of -SH reactivity (scavenger capacity and antioxidant property) by FAs as a supplement. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-biological Interactions
T1  - Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal
VL  - 224
SP  - 42
EP  - 50
DO  - 10.1016/j.cbi.2014.10.008
UR  - Kon_2778
ER  - 
@article{
author = "Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija M. and Penezić, Ana Z. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2014",
abstract = "Fatty acids (FAs) binding to human serum albumin (HSA) could lead to the changes of Cys-34 thiol group accessibility and reactivity, i.e. its scavenger capacity and antioxidant property. The influence of saturated, mono and poly unsaturated, and fish oil FAs binding to HSA on the carbonylation level and the reactivity of HSA-SH and HSA modified with methylglyoxal (MG-HSA-SH) was investigated. Changes of thiol group reactivity were followed by determination of pseudo first order rate constant (k') for thiols reaction with 5,5'-dithiobis(2-nitrobenzoic acid). HSA changes were monitored using native PAG electrophoresis and fluorescence spectroscopy. For FA/HSA molar ratios screening, qTLC and GC were used. FAs increase thiol group carbonylation levels from 8% to 20%. The k' values obtained for FAs-free HSA-SH and FAs-free MG-HSA-SH are almost equal (7.5 x 10(-3) and 7.7 x 10(-3) resp.). Binding of all FAs amplify the reactivity (k' values from 14.6 x 10(-3) to 26.0 x 10(-3) s(-1)) of HSA-SH group for 2-3.5 times in the order: palmitic, docosahexaenoic, fish oil extract, stearic, oleic, myristic and eicosapentaenoic acid, due to HSA conformational changes. FAs-bound MG-HSA-SH samples follow that pattern, but their k' values (from 9.8 x 10(-3) to 14.3 x 10(-3) s(-1)) were lower compared to unmodified HSA due to additional conformation changes of HSA molecules during carbonylation. Carbonylation level and reactivity of Cys34 thiol group of unmodified and carbonylated HSA depend on type of FAs bound to HSA, which implies the possibility for modulation of -SH reactivity (scavenger capacity and antioxidant property) by FAs as a supplement. (C) 2014 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-biological Interactions",
title = "Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal",
volume = "224",
pages = "42-50",
doi = "10.1016/j.cbi.2014.10.008",
url = "Kon_2778"
}
Pavićević, I. D., Jovanović, V. B., Takić, M. M., Penezić, A. Z., Aćimović, J. M.,& Mandić, L. M.. (2014). Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal. in Chemico-biological Interactions
Elsevier Ireland Ltd, Clare., 224, 42-50.
https://doi.org/10.1016/j.cbi.2014.10.008
Kon_2778
Pavićević ID, Jovanović VB, Takić MM, Penezić AZ, Aćimović JM, Mandić LM. Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal. in Chemico-biological Interactions. 2014;224:42-50.
doi:10.1016/j.cbi.2014.10.008
Kon_2778 .
Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija M., Penezić, Ana Z., Aćimović, Jelena M., Mandić, Ljuba M., "Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal" in Chemico-biological Interactions, 224 (2014):42-50,
https://doi.org/10.1016/j.cbi.2014.10.008 .,
Kon_2778 .
25
25
25

Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations

Vitorović-Todorović, Maja D.; Koukoulitsa, Catherine; Juranić, Ivan O.; Mandić, Ljuba M.; Drakulić, Branko J.

(Elsevier France-Editions Scientifiques Medicales Elsevier, Paris, 2014)

TY  - JOUR
AU  - Vitorović-Todorović, Maja D.
AU  - Koukoulitsa, Catherine
AU  - Juranić, Ivan O.
AU  - Mandić, Ljuba M.
AU  - Drakulić, Branko J.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1808
AB  - Congeneric set of thirty-eight 4-aryl-4-oxo-2-(N-aryl/cycloalkyl)butanamides has been designed, synthesized and evaluated for acetyl- and butyrylcholinesterase inhibitory activity. Structural variations included cycloalkylamino group attached to C2 position of butanoyl moiety, and variation of amido moiety of molecules. Twelve compounds, mostly piperidino and imidazolo derivatives, inhibited AChE in low micromolar range, and were inactive toward BChE. Several N-methylpiperazino derivatives showed inhibition of BChE in low micromolar or submicromolar concentrations, and were inactive toward AChE. Therefore, the nature of the cycloalkylamino moiety governs the AChE/BChE selectivity profile of compounds. The most active AChE inhibitor showed mixed-type inhibition modality, indicating its binding to free enzyme and to enzyme-substrate complex. Thorough docking calculations of the seven most potent AChE inhibitors from the set, showed that the hydrogen bond can be formed between amide -NH- moiety of compounds and -OH group of Tyr 124. The 10 ns unconstrained molecular dynamic simulation of the AChE- compound 18 complex shows that this interaction is the most persistent. This is, probably, the major anchoring point for the binding. (C) 2014 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations
VL  - 81
SP  - 158
EP  - 175
DO  - 10.1016/j.ejmech.2014.05.008
UR  - Kon_2691
ER  - 
@article{
author = "Vitorović-Todorović, Maja D. and Koukoulitsa, Catherine and Juranić, Ivan O. and Mandić, Ljuba M. and Drakulić, Branko J.",
year = "2014",
abstract = "Congeneric set of thirty-eight 4-aryl-4-oxo-2-(N-aryl/cycloalkyl)butanamides has been designed, synthesized and evaluated for acetyl- and butyrylcholinesterase inhibitory activity. Structural variations included cycloalkylamino group attached to C2 position of butanoyl moiety, and variation of amido moiety of molecules. Twelve compounds, mostly piperidino and imidazolo derivatives, inhibited AChE in low micromolar range, and were inactive toward BChE. Several N-methylpiperazino derivatives showed inhibition of BChE in low micromolar or submicromolar concentrations, and were inactive toward AChE. Therefore, the nature of the cycloalkylamino moiety governs the AChE/BChE selectivity profile of compounds. The most active AChE inhibitor showed mixed-type inhibition modality, indicating its binding to free enzyme and to enzyme-substrate complex. Thorough docking calculations of the seven most potent AChE inhibitors from the set, showed that the hydrogen bond can be formed between amide -NH- moiety of compounds and -OH group of Tyr 124. The 10 ns unconstrained molecular dynamic simulation of the AChE- compound 18 complex shows that this interaction is the most persistent. This is, probably, the major anchoring point for the binding. (C) 2014 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations",
volume = "81",
pages = "158-175",
doi = "10.1016/j.ejmech.2014.05.008",
url = "Kon_2691"
}
Vitorović-Todorović, M. D., Koukoulitsa, C., Juranić, I. O., Mandić, L. M.,& Drakulić, B. J.. (2014). Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 81, 158-175.
https://doi.org/10.1016/j.ejmech.2014.05.008
Kon_2691
Vitorović-Todorović MD, Koukoulitsa C, Juranić IO, Mandić LM, Drakulić BJ. Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations. in European Journal of Medicinal Chemistry. 2014;81:158-175.
doi:10.1016/j.ejmech.2014.05.008
Kon_2691 .
Vitorović-Todorović, Maja D., Koukoulitsa, Catherine, Juranić, Ivan O., Mandić, Ljuba M., Drakulić, Branko J., "Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations" in European Journal of Medicinal Chemistry, 81 (2014):158-175,
https://doi.org/10.1016/j.ejmech.2014.05.008 .,
Kon_2691 .
18
16
16

Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008

Vitorović-Todorović, Maja D.; Koukoulitsa, Catherine; Juranić, Ivan O.; Mandić, Ljuba M.; Drakulić, Branko J.

(Elsevier France-Editions Scientifiques Medicales Elsevier, Paris, 2014)

TY  - DATA
AU  - Vitorović-Todorović, Maja D.
AU  - Koukoulitsa, Catherine
AU  - Juranić, Ivan O.
AU  - Mandić, Ljuba M.
AU  - Drakulić, Branko J.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2906
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008
UR  - Kon_2691
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2906
ER  - 
@misc{
author = "Vitorović-Todorović, Maja D. and Koukoulitsa, Catherine and Juranić, Ivan O. and Mandić, Ljuba M. and Drakulić, Branko J.",
year = "2014",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008",
url = "Kon_2691, https://hdl.handle.net/21.15107/rcub_cherry_2906"
}
Vitorović-Todorović, M. D., Koukoulitsa, C., Juranić, I. O., Mandić, L. M.,& Drakulić, B. J.. (2014). Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris..
Kon_2691
Vitorović-Todorović MD, Koukoulitsa C, Juranić IO, Mandić LM, Drakulić BJ. Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008. in European Journal of Medicinal Chemistry. 2014;.
Kon_2691 .
Vitorović-Todorović, Maja D., Koukoulitsa, Catherine, Juranić, Ivan O., Mandić, Ljuba M., Drakulić, Branko J., "Supplementary data for the article: Vitorović-Todorović, M. D.; Koukoulitsa, C.; Juranić, I. O.; Mandić, L. M.; Drakulić, B. J. Structural Modifications of 4-Aryl-4-Oxo-2-Aminylbutanamides and Their Acetyl- and Butyrylcholinesterase Inhibitory Activity. Investigation of AChE-Ligand Interactions by Docking Calculations and Molecular Dynamics Simulations. European Journal of Medicinal Chemistry 2014, 81, 158–175. https://doi.org/10.1016/j.ejmech.2014.05.008" in European Journal of Medicinal Chemistry (2014),
Kon_2691 .

Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations

Vitorović-Todorović, Maja D.; Koukoulitsa, Catherine; Juranić, Ivan O.; Mandić, Ljuba M.; Drakulić, Branko J.

(Elsevier France-Editions Scientifiques Medicales Elsevier, Paris, 2014)

TY  - JOUR
AU  - Vitorović-Todorović, Maja D.
AU  - Koukoulitsa, Catherine
AU  - Juranić, Ivan O.
AU  - Mandić, Ljuba M.
AU  - Drakulić, Branko J.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2907
AB  - Congeneric set of thirty-eight 4-aryl-4-oxo-2-(N-aryl/cycloalkyl)butanamides has been designed, synthesized and evaluated for acetyl- and butyrylcholinesterase inhibitory activity. Structural variations included cycloalkylamino group attached to C2 position of butanoyl moiety, and variation of amido moiety of molecules. Twelve compounds, mostly piperidino and imidazolo derivatives, inhibited AChE in low micromolar range, and were inactive toward BChE. Several N-methylpiperazino derivatives showed inhibition of BChE in low micromolar or submicromolar concentrations, and were inactive toward AChE. Therefore, the nature of the cycloalkylamino moiety governs the AChE/BChE selectivity profile of compounds. The most active AChE inhibitor showed mixed-type inhibition modality, indicating its binding to free enzyme and to enzyme-substrate complex. Thorough docking calculations of the seven most potent AChE inhibitors from the set, showed that the hydrogen bond can be formed between amide -NH- moiety of compounds and -OH group of Tyr 124. The 10 ns unconstrained molecular dynamic simulation of the AChE- compound 18 complex shows that this interaction is the most persistent. This is, probably, the major anchoring point for the binding. (C) 2014 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations
VL  - 81
SP  - 158
EP  - 175
DO  - 10.1016/j.ejmech.2014.05.008
UR  - Kon_2691
ER  - 
@article{
author = "Vitorović-Todorović, Maja D. and Koukoulitsa, Catherine and Juranić, Ivan O. and Mandić, Ljuba M. and Drakulić, Branko J.",
year = "2014",
abstract = "Congeneric set of thirty-eight 4-aryl-4-oxo-2-(N-aryl/cycloalkyl)butanamides has been designed, synthesized and evaluated for acetyl- and butyrylcholinesterase inhibitory activity. Structural variations included cycloalkylamino group attached to C2 position of butanoyl moiety, and variation of amido moiety of molecules. Twelve compounds, mostly piperidino and imidazolo derivatives, inhibited AChE in low micromolar range, and were inactive toward BChE. Several N-methylpiperazino derivatives showed inhibition of BChE in low micromolar or submicromolar concentrations, and were inactive toward AChE. Therefore, the nature of the cycloalkylamino moiety governs the AChE/BChE selectivity profile of compounds. The most active AChE inhibitor showed mixed-type inhibition modality, indicating its binding to free enzyme and to enzyme-substrate complex. Thorough docking calculations of the seven most potent AChE inhibitors from the set, showed that the hydrogen bond can be formed between amide -NH- moiety of compounds and -OH group of Tyr 124. The 10 ns unconstrained molecular dynamic simulation of the AChE- compound 18 complex shows that this interaction is the most persistent. This is, probably, the major anchoring point for the binding. (C) 2014 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations",
volume = "81",
pages = "158-175",
doi = "10.1016/j.ejmech.2014.05.008",
url = "Kon_2691"
}
Vitorović-Todorović, M. D., Koukoulitsa, C., Juranić, I. O., Mandić, L. M.,& Drakulić, B. J.. (2014). Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 81, 158-175.
https://doi.org/10.1016/j.ejmech.2014.05.008
Kon_2691
Vitorović-Todorović MD, Koukoulitsa C, Juranić IO, Mandić LM, Drakulić BJ. Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations. in European Journal of Medicinal Chemistry. 2014;81:158-175.
doi:10.1016/j.ejmech.2014.05.008
Kon_2691 .
Vitorović-Todorović, Maja D., Koukoulitsa, Catherine, Juranić, Ivan O., Mandić, Ljuba M., Drakulić, Branko J., "Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- and butyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations" in European Journal of Medicinal Chemistry, 81 (2014):158-175,
https://doi.org/10.1016/j.ejmech.2014.05.008 .,
Kon_2691 .
18
16
16

How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?

Jovanović, Vesna B.; Aćimović, Jelena M.; Sreckovic, Vesna S. Dimitrijevic; Mandić, Ljuba M.

(Serbian Chemical Soc, Belgrade, 2014)

TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Aćimović, Jelena M.
AU  - Sreckovic, Vesna S. Dimitrijevic
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1647
AB  - It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P  lt  0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?
VL  - 79
IS  - 12
SP  - 1491
EP  - 1503
DO  - 10.2298/JSC140430076J
UR  - Kon_2793
ER  - 
@article{
author = "Jovanović, Vesna B. and Aćimović, Jelena M. and Sreckovic, Vesna S. Dimitrijevic and Mandić, Ljuba M.",
year = "2014",
abstract = "It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P  lt  0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?",
volume = "79",
number = "12",
pages = "1491-1503",
doi = "10.2298/JSC140430076J",
url = "Kon_2793"
}
Jovanović, V. B., Aćimović, J. M., Sreckovic, V. S. D.,& Mandić, L. M.. (2014). How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 79(12), 1491-1503.
https://doi.org/10.2298/JSC140430076J
Kon_2793
Jovanović VB, Aćimović JM, Sreckovic VSD, Mandić LM. How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society. 2014;79(12):1491-1503.
doi:10.2298/JSC140430076J
Kon_2793 .
Jovanović, Vesna B., Aćimović, Jelena M., Sreckovic, Vesna S. Dimitrijevic, Mandić, Ljuba M., "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?" in Journal of the Serbian Chemical Society, 79, no. 12 (2014):1491-1503,
https://doi.org/10.2298/JSC140430076J .,
Kon_2793 .

Improving the reliability of human serum albumin-thiol group determination

Jovanović, Vesna B.; Penezić-Romanjuk, Ana Z.; Pavićević, Ivan D.; Aćimović, Jelena M.; Mandić, Ljuba M.

(Academic Press Inc Elsevier Science, San Diego, 2013)

TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Penezić-Romanjuk, Ana Z.
AU  - Pavićević, Ivan D.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1369
AB  - The thiol (Cys34) content of human serum albumin (HSA-SH) decreases during oxidative and carbonyl stress and, therefore, could represent a useful parameter in clinical practice. Nevertheless, the reliability of HSA-thiol determination with Ellman's method depends on the purity of isolated HSA. Determination of total serum thiols (mmol/L) and HSA-SH content (mmol -SH/mmol HSA) after HSA isolation from diabetic patient and control sera by a two-step precipitation with ammonium sulfate (AS), as well as HSA-SH contribution (%) to total serum thiols, was assessed. Purity and yield of isolated HSA were monitored spectrophotometrically and by native polyacrylamide gel electrophoresis. Precipitation of HSA from serum via a two-step method with AS produced HSA with 91.9 +/- 3.6% purity and 69.7 +/- 4.4% yield, allowing for precise (relative standard deviation of 3.2%) and reliable (comparing with total serum thiols) measurement of HSA-SH content with DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)]. The content of the HSA-SH group in patients with type 2 diabetes was significantly (P  lt  0.05) lower compared with that of the healthy cohort (0.483 +/- 0.067 vs. 0.561 +/- 0.054 mmol -SH/mmol HSA). Because the proposed method of HSA isolation is simple, time-efficient, and technically less demanding, and it also enables reliable determination of HSA-SH content, it is suitable for clinical practice.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Improving the reliability of human serum albumin-thiol group determination
VL  - 439
IS  - 1
SP  - 17
EP  - 22
DO  - 10.1016/j.ab.2013.03.033
UR  - Kon_2489
ER  - 
@article{
author = "Jovanović, Vesna B. and Penezić-Romanjuk, Ana Z. and Pavićević, Ivan D. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2013",
abstract = "The thiol (Cys34) content of human serum albumin (HSA-SH) decreases during oxidative and carbonyl stress and, therefore, could represent a useful parameter in clinical practice. Nevertheless, the reliability of HSA-thiol determination with Ellman's method depends on the purity of isolated HSA. Determination of total serum thiols (mmol/L) and HSA-SH content (mmol -SH/mmol HSA) after HSA isolation from diabetic patient and control sera by a two-step precipitation with ammonium sulfate (AS), as well as HSA-SH contribution (%) to total serum thiols, was assessed. Purity and yield of isolated HSA were monitored spectrophotometrically and by native polyacrylamide gel electrophoresis. Precipitation of HSA from serum via a two-step method with AS produced HSA with 91.9 +/- 3.6% purity and 69.7 +/- 4.4% yield, allowing for precise (relative standard deviation of 3.2%) and reliable (comparing with total serum thiols) measurement of HSA-SH content with DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)]. The content of the HSA-SH group in patients with type 2 diabetes was significantly (P  lt  0.05) lower compared with that of the healthy cohort (0.483 +/- 0.067 vs. 0.561 +/- 0.054 mmol -SH/mmol HSA). Because the proposed method of HSA isolation is simple, time-efficient, and technically less demanding, and it also enables reliable determination of HSA-SH content, it is suitable for clinical practice.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Improving the reliability of human serum albumin-thiol group determination",
volume = "439",
number = "1",
pages = "17-22",
doi = "10.1016/j.ab.2013.03.033",
url = "Kon_2489"
}
Jovanović, V. B., Penezić-Romanjuk, A. Z., Pavićević, I. D., Aćimović, J. M.,& Mandić, L. M.. (2013). Improving the reliability of human serum albumin-thiol group determination. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 439(1), 17-22.
https://doi.org/10.1016/j.ab.2013.03.033
Kon_2489
Jovanović VB, Penezić-Romanjuk AZ, Pavićević ID, Aćimović JM, Mandić LM. Improving the reliability of human serum albumin-thiol group determination. in Analytical Biochemistry. 2013;439(1):17-22.
doi:10.1016/j.ab.2013.03.033
Kon_2489 .
Jovanović, Vesna B., Penezić-Romanjuk, Ana Z., Pavićević, Ivan D., Aćimović, Jelena M., Mandić, Ljuba M., "Improving the reliability of human serum albumin-thiol group determination" in Analytical Biochemistry, 439, no. 1 (2013):17-22,
https://doi.org/10.1016/j.ab.2013.03.033 .,
Kon_2489 .
14
12
14

Monitoring of the human serum albumin carbonylation level through determination of guanidino group content

Aćimović, Jelena M.; Jovanović, Vesna B.; Sreckovic, Vesna Dimitrijevic; Penezić-Romanjuk, Ana Z.; Mandić, Ljuba M.

(Academic Press Inc Elsevier Science, San Diego, 2013)

TY  - JOUR
AU  - Aćimović, Jelena M.
AU  - Jovanović, Vesna B.
AU  - Sreckovic, Vesna Dimitrijevic
AU  - Penezić-Romanjuk, Ana Z.
AU  - Mandić, Ljuba M.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1578
AB  - Carbonylation of the protein amino, guanidine, and thiol groups with alpha-oxoaldehydes (which are produced in higher quantities in diabetes, uremia, oxidative stress, aging, and inflammation) is one of the important causes of vascular complications. For monitoring of the human serum albumin (HSA) carbonylation level, a spectrophotometric method based on the formation of colored adduct between guanidine group and thymol-sodium hypobromite reagent in the alkaline medium was investigated. Beer's law is obeyed in the concentration range of Arg and protein guanidine groups from 1 to 40 mM. Precision of the method (relative standard deviation) was in the range of 0.9 to 2%. Accuracy was examined by the standard addition method (recovery similar to 100%). The method was applied for monitoring of the carbonylation level of HSA with methylglyoxal in vitro and of HSA isolated (using affinity chromatography) from sera of 21 patients with type 2 diabetes and 12 healthy persons. The content of guanidine groups in HSA isolated from diabetics (19.64 +/- 1.07 mM/mM albumin) was significantly lower (P  lt  0.001) in comparison with a control group (21.87 +/- 1.02 mM/mM albumin). The method is simple and fast, has good accuracy and precision, and is suitable for clinical practice as well for in vitro protein carbonylation experiments.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Monitoring of the human serum albumin carbonylation level through determination of guanidino group content
VL  - 433
IS  - 2
SP  - 162
EP  - 167
DO  - 10.1016/j.ab.2012.10.028
UR  - Kon_2409
ER  - 
@article{
author = "Aćimović, Jelena M. and Jovanović, Vesna B. and Sreckovic, Vesna Dimitrijevic and Penezić-Romanjuk, Ana Z. and Mandić, Ljuba M.",
year = "2013",
abstract = "Carbonylation of the protein amino, guanidine, and thiol groups with alpha-oxoaldehydes (which are produced in higher quantities in diabetes, uremia, oxidative stress, aging, and inflammation) is one of the important causes of vascular complications. For monitoring of the human serum albumin (HSA) carbonylation level, a spectrophotometric method based on the formation of colored adduct between guanidine group and thymol-sodium hypobromite reagent in the alkaline medium was investigated. Beer's law is obeyed in the concentration range of Arg and protein guanidine groups from 1 to 40 mM. Precision of the method (relative standard deviation) was in the range of 0.9 to 2%. Accuracy was examined by the standard addition method (recovery similar to 100%). The method was applied for monitoring of the carbonylation level of HSA with methylglyoxal in vitro and of HSA isolated (using affinity chromatography) from sera of 21 patients with type 2 diabetes and 12 healthy persons. The content of guanidine groups in HSA isolated from diabetics (19.64 +/- 1.07 mM/mM albumin) was significantly lower (P  lt  0.001) in comparison with a control group (21.87 +/- 1.02 mM/mM albumin). The method is simple and fast, has good accuracy and precision, and is suitable for clinical practice as well for in vitro protein carbonylation experiments.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Monitoring of the human serum albumin carbonylation level through determination of guanidino group content",
volume = "433",
number = "2",
pages = "162-167",
doi = "10.1016/j.ab.2012.10.028",
url = "Kon_2409"
}
Aćimović, J. M., Jovanović, V. B., Sreckovic, V. D., Penezić-Romanjuk, A. Z.,& Mandić, L. M.. (2013). Monitoring of the human serum albumin carbonylation level through determination of guanidino group content. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 433(2), 162-167.
https://doi.org/10.1016/j.ab.2012.10.028
Kon_2409
Aćimović JM, Jovanović VB, Sreckovic VD, Penezić-Romanjuk AZ, Mandić LM. Monitoring of the human serum albumin carbonylation level through determination of guanidino group content. in Analytical Biochemistry. 2013;433(2):162-167.
doi:10.1016/j.ab.2012.10.028
Kon_2409 .
Aćimović, Jelena M., Jovanović, Vesna B., Sreckovic, Vesna Dimitrijevic, Penezić-Romanjuk, Ana Z., Mandić, Ljuba M., "Monitoring of the human serum albumin carbonylation level through determination of guanidino group content" in Analytical Biochemistry, 433, no. 2 (2013):162-167,
https://doi.org/10.1016/j.ab.2012.10.028 .,
Kon_2409 .
6
6
6

Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase

Stankov-Jovanović, Vesna P.; Mitić, Violeta D.; Ilić, Marija D.; Mandić, Ljuba M.; Nikolić-Mandić, Snežana D.

(Assoc Chemists & Chemical Engineers Of Serbia, Belgrade, 2012)

TY  - JOUR
AU  - Stankov-Jovanović, Vesna P.
AU  - Mitić, Violeta D.
AU  - Ilić, Marija D.
AU  - Mandić, Ljuba M.
AU  - Nikolić-Mandić, Snežana D.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1598
AB  - Propranolol, a widely used beta-blocker, inhibits the hydrolysis reaction of enzyme cholinesterase. Measurements of the difference in rate of hydrolysis rate between uninhibited and inhibited reactions allow the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (K-M, 0.326-0.330 mmol/L; V-max, 40.0-43.0 mu mol/Lmin). The inhibition type was recognized as competitive and the inhibition constant, Ki, was determined to be 22.60 mu mol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 mu mol/L, respectively. Accuracy and precision of proposed methods were tested. The proposed method showed good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications.
AB  - Za propranolol, često propisivani neselektivni beta blokator, utvrđeno je da inhibira reakciju enzimske hidrolize butiriltioholin-jodida, koja je katalizovana serumskom holinesterazom. Merenjem razlike u brzini osnovne i inhibitorske reakcije hidrolize u prisustvu propranolola kao inhibitora, moguće je razviti kinetičku metodu za određivanje propranolola. Oba sistema, enzim-supstrat-hromogen kao i enzim-supstrat-hromogen-inhibitor, okarakterisani su biohemijskim kinetičkim parametrima (KM, 0,326-0,330 mmol/L; Vmax, 40-42,99 μmol/L min), inhibicija je definisana kao kompetitivna i određena je konstanta inhibicije 22,60 μmol/L. Da bi se u potpunosti iskoristile sve mogućnosti predložene metode u pogledu osetljivosti, tačnosti, preciznosti i selektivnosti, optimizovani su reakcioni uslovi. Konstruisana je kalibraciona prava, izračunata odgovarajuća jednačina i određeni granica detekcije i kvantifikacije i to 0,004 i 0,0136 μmol/L, redom. Tačnost i preciznost predložene metode su ispitane za tri koncentracije propranolola u oblasti kalibracione prave (0,082-21,120 μmol/L) u pet ponavljanja. Takođe, ispitan je uticaj većeg broja supstanci koje se mogu naći u uzorku na brzinu reakcije. Optimizovana metoda je primenjena za određivanje propranolola u farmaceutskim preparatima. Tačnost predložene metode je ispitana primenom metode standardnog dodatka. Predložena metoda ima dobru osetljivost, selektivnost, jednostavna je i brza, i nadasve lako dostupna, i na taj način primenljiva u velikom broju laboratorija.
PB  - Assoc Chemists & Chemical Engineers Of Serbia, Belgrade
T2  - Hemijska industrija
T1  - Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase
T1  - Enzimska kinetička metoda za određivanje propranolol-hidrohlorida u farmaceutskim preparatima zasnovana na njegovom inhibitorskom delovanju na holinesterazu
VL  - 66
IS  - 5
SP  - 677
EP  - 684
DO  - 10.2298/HEMIND120128032S
UR  - Kon_2429
ER  - 
@article{
author = "Stankov-Jovanović, Vesna P. and Mitić, Violeta D. and Ilić, Marija D. and Mandić, Ljuba M. and Nikolić-Mandić, Snežana D.",
year = "2012",
abstract = "Propranolol, a widely used beta-blocker, inhibits the hydrolysis reaction of enzyme cholinesterase. Measurements of the difference in rate of hydrolysis rate between uninhibited and inhibited reactions allow the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (K-M, 0.326-0.330 mmol/L; V-max, 40.0-43.0 mu mol/Lmin). The inhibition type was recognized as competitive and the inhibition constant, Ki, was determined to be 22.60 mu mol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 mu mol/L, respectively. Accuracy and precision of proposed methods were tested. The proposed method showed good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications., Za propranolol, često propisivani neselektivni beta blokator, utvrđeno je da inhibira reakciju enzimske hidrolize butiriltioholin-jodida, koja je katalizovana serumskom holinesterazom. Merenjem razlike u brzini osnovne i inhibitorske reakcije hidrolize u prisustvu propranolola kao inhibitora, moguće je razviti kinetičku metodu za određivanje propranolola. Oba sistema, enzim-supstrat-hromogen kao i enzim-supstrat-hromogen-inhibitor, okarakterisani su biohemijskim kinetičkim parametrima (KM, 0,326-0,330 mmol/L; Vmax, 40-42,99 μmol/L min), inhibicija je definisana kao kompetitivna i određena je konstanta inhibicije 22,60 μmol/L. Da bi se u potpunosti iskoristile sve mogućnosti predložene metode u pogledu osetljivosti, tačnosti, preciznosti i selektivnosti, optimizovani su reakcioni uslovi. Konstruisana je kalibraciona prava, izračunata odgovarajuća jednačina i određeni granica detekcije i kvantifikacije i to 0,004 i 0,0136 μmol/L, redom. Tačnost i preciznost predložene metode su ispitane za tri koncentracije propranolola u oblasti kalibracione prave (0,082-21,120 μmol/L) u pet ponavljanja. Takođe, ispitan je uticaj većeg broja supstanci koje se mogu naći u uzorku na brzinu reakcije. Optimizovana metoda je primenjena za određivanje propranolola u farmaceutskim preparatima. Tačnost predložene metode je ispitana primenom metode standardnog dodatka. Predložena metoda ima dobru osetljivost, selektivnost, jednostavna je i brza, i nadasve lako dostupna, i na taj način primenljiva u velikom broju laboratorija.",
publisher = "Assoc Chemists & Chemical Engineers Of Serbia, Belgrade",
journal = "Hemijska industrija",
title = "Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase, Enzimska kinetička metoda za određivanje propranolol-hidrohlorida u farmaceutskim preparatima zasnovana na njegovom inhibitorskom delovanju na holinesterazu",
volume = "66",
number = "5",
pages = "677-684",
doi = "10.2298/HEMIND120128032S",
url = "Kon_2429"
}
Stankov-Jovanović, V. P., Mitić, V. D., Ilić, M. D., Mandić, L. M.,& Nikolić-Mandić, S. D.. (2012). Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase. in Hemijska industrija
Assoc Chemists & Chemical Engineers Of Serbia, Belgrade., 66(5), 677-684.
https://doi.org/10.2298/HEMIND120128032S
Kon_2429
Stankov-Jovanović VP, Mitić VD, Ilić MD, Mandić LM, Nikolić-Mandić SD. Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase. in Hemijska industrija. 2012;66(5):677-684.
doi:10.2298/HEMIND120128032S
Kon_2429 .
Stankov-Jovanović, Vesna P., Mitić, Violeta D., Ilić, Marija D., Mandić, Ljuba M., Nikolić-Mandić, Snežana D., "Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase" in Hemijska industrija, 66, no. 5 (2012):677-684,
https://doi.org/10.2298/HEMIND120128032S .,
Kon_2429 .
3
2

Chronic isolation stress compromises JNK/c-Jun signaling in rat brain

Filipović, Dragana; Zlatkovic, Jelena; Pavićević, Ivan D.; Mandić, Ljuba M.; Demajo, Miroslav

(Springer Wien, Wien, 2012)

TY  - JOUR
AU  - Filipović, Dragana
AU  - Zlatkovic, Jelena
AU  - Pavićević, Ivan D.
AU  - Mandić, Ljuba M.
AU  - Demajo, Miroslav
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1540
AB  - The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.
PB  - Springer Wien, Wien
T2  - Journal of Neural Transmission
T1  - Chronic isolation stress compromises JNK/c-Jun signaling in rat brain
VL  - 119
IS  - 11
SP  - 1275
EP  - 1284
DO  - 10.1007/s00702-012-0776-0
UR  - Kon_2371
ER  - 
@article{
author = "Filipović, Dragana and Zlatkovic, Jelena and Pavićević, Ivan D. and Mandić, Ljuba M. and Demajo, Miroslav",
year = "2012",
abstract = "The c-Jun NH2-terminal kinases (JNKs) are important stress-responsive kinases. They regulate cellular activities by sequential phosphorylation and activation through a mitogen-activated protein kinase cascade, whereas JNKs activation is altered in response to various stressors. In the present study, we used immunoblotting to assess the effect of 21 day of social isolation as the chronic stressor, either sole and in combination with 2 h of acute immobilization or cold (4A degrees C) stress on circulating corticosterone level and phosphorylation status of p46 (phospho-p46/total p46) and p54 (phospho-p54/total p54) JNK isoforms in the cytosolic and nuclear fraction of the prefrontal cortex and hippocampus of male Wistar rats. Also, the phosphorylation status of JNK nuclear down-stream target c-Jun (p-c-Jun/c-Jun) on Ser63 was examined. Both acute stressors with elevated CORT levels led to increased phosphorylation status of cytosolic p54 JNK isoforms but not p46 JNK isoforms only in the hippocampus and no change in phosphorylation status of c-jun in both brain regions. Chronic isolation with unaltered CORT level and reduced responsiveness to novel acute stressors, led to unchanged or reduced phosphorylation status of p46 and p54 JNK isoforms in both fractions and both brain regions, whereas the decrease of c-Jun phosphorylation status was found only in the prefrontal cortex. Our results suggest that compromised JNKs activation following chronic isolation may lead to interruption of JNK signaling, which could be related with neuropsychiatric disorders such as depression or long-lasting neuronal remodeling.",
publisher = "Springer Wien, Wien",
journal = "Journal of Neural Transmission",
title = "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain",
volume = "119",
number = "11",
pages = "1275-1284",
doi = "10.1007/s00702-012-0776-0",
url = "Kon_2371"
}
Filipović, D., Zlatkovic, J., Pavićević, I. D., Mandić, L. M.,& Demajo, M.. (2012). Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission
Springer Wien, Wien., 119(11), 1275-1284.
https://doi.org/10.1007/s00702-012-0776-0
Kon_2371
Filipović D, Zlatkovic J, Pavićević ID, Mandić LM, Demajo M. Chronic isolation stress compromises JNK/c-Jun signaling in rat brain. in Journal of Neural Transmission. 2012;119(11):1275-1284.
doi:10.1007/s00702-012-0776-0
Kon_2371 .
Filipović, Dragana, Zlatkovic, Jelena, Pavićević, Ivan D., Mandić, Ljuba M., Demajo, Miroslav, "Chronic isolation stress compromises JNK/c-Jun signaling in rat brain" in Journal of Neural Transmission, 119, no. 11 (2012):1275-1284,
https://doi.org/10.1007/s00702-012-0776-0 .,
Kon_2371 .
6
3
4