TY  - JOUR
AU  - Pompach, Petr
AU  - Viola, Cristina M.
AU  - Radosavljević, Jelena
AU  - Lin, Jingjing
AU  - Jiráček, Jirˇí
AU  - Brzozowski, Andrzej M.
AU  - Selicharová, Irena
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3719
AB  - Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques and reveal details of these protein-protein interfaces. We used such approach to clarify missing structural description of the interface in human insulin-like growth factor (IGF-1): Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray crystallography. We crosslinked these proteins by heterobifunctional cross-linker sulfosuccinimidyl 4,4′-azidopentanoate (Sulfo-SDA) for the subsequent mass spectrometry (MS) analysis. The MS analysis revealed IGF-1:Imp-L2 interactions which were not resolved in the crystal structure of this assembly, and they converged with X-ray results, indicating the importance of the IGF-1 N-terminus interaction with the C-terminal (185–242) part of the Imp-L2 for stability of this complex. Here, we also showed the advantage and reliability of MS approach in solving details of protein-protein interactions that are too flexible for solid state structural methods.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2
VL  - 10
DO  - 10.3389/fendo.2019.00695
ER  - 

@article{
author = "Pompach, Petr and Viola, Cristina M. and Radosavljević, Jelena and Lin, Jingjing and Jiráček, Jirˇí and Brzozowski, Andrzej M. and Selicharová, Irena",
year = "2019",
abstract = "Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques and reveal details of these protein-protein interfaces. We used such approach to clarify missing structural description of the interface in human insulin-like growth factor (IGF-1): Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray crystallography. We crosslinked these proteins by heterobifunctional cross-linker sulfosuccinimidyl 4,4′-azidopentanoate (Sulfo-SDA) for the subsequent mass spectrometry (MS) analysis. The MS analysis revealed IGF-1:Imp-L2 interactions which were not resolved in the crystal structure of this assembly, and they converged with X-ray results, indicating the importance of the IGF-1 N-terminus interaction with the C-terminal (185–242) part of the Imp-L2 for stability of this complex. Here, we also showed the advantage and reliability of MS approach in solving details of protein-protein interactions that are too flexible for solid state structural methods.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2",
volume = "10",
doi = "10.3389/fendo.2019.00695"
}

Pompach, P., Viola, C. M., Radosavljević, J., Lin, J., Jiráček, J., Brzozowski, A. M.,& Selicharová, I.. (2019). Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. in Frontiers in Endocrinology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fendo.2019.00695

Pompach P, Viola CM, Radosavljević J, Lin J, Jiráček J, Brzozowski AM, Selicharová I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. in Frontiers in Endocrinology. 2019;10.
doi:10.3389/fendo.2019.00695 .

Pompach, Petr, Viola, Cristina M., Radosavljević, Jelena, Lin, Jingjing, Jiráček, Jirˇí, Brzozowski, Andrzej M., Selicharová, Irena, "Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2" in Frontiers in Endocrinology, 10 (2019),
https://doi.org/10.3389/fendo.2019.00695 . .

TY  - DATA
AU  - Pompach, Petr
AU  - Viola, Cristina M.
AU  - Radosavljević, Jelena
AU  - Lin, Jingjing
AU  - Jiráček, Jirˇí
AU  - Brzozowski, Andrzej M.
AU  - Selicharová, Irena
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3720
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Supplementary data for the article: Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3720
ER  - 

@misc{
author = "Pompach, Petr and Viola, Cristina M. and Radosavljević, Jelena and Lin, Jingjing and Jiráček, Jirˇí and Brzozowski, Andrzej M. and Selicharová, Irena",
year = "2019",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Supplementary data for the article: Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3720"
}

Pompach, P., Viola, C. M., Radosavljević, J., Lin, J., Jiráček, J., Brzozowski, A. M.,& Selicharová, I.. (2019). Supplementary data for the article: Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695. in Frontiers in Endocrinology
Frontiers Media S.A...
https://hdl.handle.net/21.15107/rcub_cherry_3720

Pompach P, Viola CM, Radosavljević J, Lin J, Jiráček J, Brzozowski AM, Selicharová I. Supplementary data for the article: Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695. in Frontiers in Endocrinology. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3720 .

Pompach, Petr, Viola, Cristina M., Radosavljević, Jelena, Lin, Jingjing, Jiráček, Jirˇí, Brzozowski, Andrzej M., Selicharová, Irena, "Supplementary data for the article: Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695" in Frontiers in Endocrinology (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3720 .