TY  - DATA
AU  - Sentić, Milica
AU  - Virgilio, Francesca
AU  - Zanut, Alessandra
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Tormen, Massimo
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3566
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1
DO  - 10.1007/s00216-016-9504-1
ER  - 

@misc{
author = "Sentić, Milica and Virgilio, Francesca and Zanut, Alessandra and Manojlović, Dragan D. and Arbault, Stephane and Tormen, Massimo and Šojić, Nešo and Ugo, Paolo",
year = "2016",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1",
doi = "10.1007/s00216-016-9504-1"
}

Sentić, M., Virgilio, F., Zanut, A., Manojlović, D. D., Arbault, S., Tormen, M., Šojić, N.,& Ugo, P.. (2016). Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg..
https://doi.org/10.1007/s00216-016-9504-1

Sentić M, Virgilio F, Zanut A, Manojlović DD, Arbault S, Tormen M, Šojić N, Ugo P. Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1. in Analytical and Bioanalytical Chemistry. 2016;.
doi:10.1007/s00216-016-9504-1 .

Sentić, Milica, Virgilio, Francesca, Zanut, Alessandra, Manojlović, Dragan D., Arbault, Stephane, Tormen, Massimo, Šojić, Nešo, Ugo, Paolo, "Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1" in Analytical and Bioanalytical Chemistry (2016),
https://doi.org/10.1007/s00216-016-9504-1 . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Virgilio, Francesca
AU  - Zanut, Alessandra
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Tormen, Massimo
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2311
AB  - Nanoelectrode arrays (NEAs) are increasingly applied for a variety of electroanalytical applications; however, very few studies dealt with the use of NEAs as an electrochemical generator of electrogenerated chemiluminescence (ECL). In the present study, arrays of nanodisc and nanoband electrodes with different dimensions and inter-electrode distances were fabricated by e-beam lithography on a polycarbonate layer deposited on boron-doped diamond (BDD) substrates. In particular, NEAs with 16 different geometries were fabricated on the same BDD sample substrate obtaining a multiple nanoelectrode and ultramicroelectrode array platform (MNEAP). After electrochemical and morphological characterization, the MNEAP was used to capture simultaneously with a single image the characteristic behaviour of ECL emission from all the 16 arrays. Experiments were performed using Ru(bpy)(3) (2+) as the ECL luminophore and tri-n-propylamine (TPrA) as the co-reactant. With a relatively limited number of experiments, such an imaging procedure allowed to study the role that geometrical and mechanistic parameters play on ECL generation at NEAs. In particular, at high concentrations of TPrA, well-separated individual ECL spots or bands revealed an ECL signal which forms a pattern matching the nanofabricated structure. The analysis of the imaging data indicated that the thickness of the ECL-emitting zone at each nanoelectrode scales inversely with the co-reactant concentration, while significantly stronger ECL signals were detected for NEAs operating under overlap conditions.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays
VL  - 408
IS  - 25
SP  - 7085
EP  - 7094
DO  - 10.1007/s00216-016-9504-1
ER  - 

@article{
author = "Sentić, Milica and Virgilio, Francesca and Zanut, Alessandra and Manojlović, Dragan D. and Arbault, Stephane and Tormen, Massimo and Šojić, Nešo and Ugo, Paolo",
year = "2016",
abstract = "Nanoelectrode arrays (NEAs) are increasingly applied for a variety of electroanalytical applications; however, very few studies dealt with the use of NEAs as an electrochemical generator of electrogenerated chemiluminescence (ECL). In the present study, arrays of nanodisc and nanoband electrodes with different dimensions and inter-electrode distances were fabricated by e-beam lithography on a polycarbonate layer deposited on boron-doped diamond (BDD) substrates. In particular, NEAs with 16 different geometries were fabricated on the same BDD sample substrate obtaining a multiple nanoelectrode and ultramicroelectrode array platform (MNEAP). After electrochemical and morphological characterization, the MNEAP was used to capture simultaneously with a single image the characteristic behaviour of ECL emission from all the 16 arrays. Experiments were performed using Ru(bpy)(3) (2+) as the ECL luminophore and tri-n-propylamine (TPrA) as the co-reactant. With a relatively limited number of experiments, such an imaging procedure allowed to study the role that geometrical and mechanistic parameters play on ECL generation at NEAs. In particular, at high concentrations of TPrA, well-separated individual ECL spots or bands revealed an ECL signal which forms a pattern matching the nanofabricated structure. The analysis of the imaging data indicated that the thickness of the ECL-emitting zone at each nanoelectrode scales inversely with the co-reactant concentration, while significantly stronger ECL signals were detected for NEAs operating under overlap conditions.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays",
volume = "408",
number = "25",
pages = "7085-7094",
doi = "10.1007/s00216-016-9504-1"
}

Sentić, M., Virgilio, F., Zanut, A., Manojlović, D. D., Arbault, S., Tormen, M., Šojić, N.,& Ugo, P.. (2016). Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 408(25), 7085-7094.
https://doi.org/10.1007/s00216-016-9504-1

Sentić M, Virgilio F, Zanut A, Manojlović DD, Arbault S, Tormen M, Šojić N, Ugo P. Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays. in Analytical and Bioanalytical Chemistry. 2016;408(25):7085-7094.
doi:10.1007/s00216-016-9504-1 .

Sentić, Milica, Virgilio, Francesca, Zanut, Alessandra, Manojlović, Dragan D., Arbault, Stephane, Tormen, Massimo, Šojić, Nešo, Ugo, Paolo, "Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays" in Analytical and Bioanalytical Chemistry, 408, no. 25 (2016):7085-7094,
https://doi.org/10.1007/s00216-016-9504-1 . .

TY  - DATA
AU  - Habtamu, Henok B.
AU  - Sentić, Milica
AU  - Silvestrini, Morena
AU  - De Leo, Luigina
AU  - Not, Tarcisio
AU  - Arbault, Stephane
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3329
PB  - Amer Chemical Soc, Washington
T2  - Analytical Chemistry
T1  - Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3329
ER  - 

@misc{
author = "Habtamu, Henok B. and Sentić, Milica and Silvestrini, Morena and De Leo, Luigina and Not, Tarcisio and Arbault, Stephane and Manojlović, Dragan D. and Šojić, Nešo and Ugo, Paolo",
year = "2015",
publisher = "Amer Chemical Soc, Washington",
journal = "Analytical Chemistry",
title = "Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3329"
}

Habtamu, H. B., Sentić, M., Silvestrini, M., De Leo, L., Not, T., Arbault, S., Manojlović, D. D., Šojić, N.,& Ugo, P.. (2015). Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801. in Analytical Chemistry
Amer Chemical Soc, Washington..
https://hdl.handle.net/21.15107/rcub_cherry_3329

Habtamu HB, Sentić M, Silvestrini M, De Leo L, Not T, Arbault S, Manojlović DD, Šojić N, Ugo P. Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801. in Analytical Chemistry. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3329 .

Habtamu, Henok B., Sentić, Milica, Silvestrini, Morena, De Leo, Luigina, Not, Tarcisio, Arbault, Stephane, Manojlović, Dragan D., Šojić, Nešo, Ugo, Paolo, "Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801" in Analytical Chemistry (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3329 .

TY  - JOUR
AU  - Habtamu, Henok B.
AU  - Sentić, Milica
AU  - Silvestrini, Morena
AU  - De Leo, Luigina
AU  - Not, Tarcisio
AU  - Arbault, Stephane
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2014
AB  - We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.
PB  - Amer Chemical Soc, Washington
T2  - Analytical Chemistry
T1  - A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles
VL  - 87
IS  - 24
SP  - 12080
EP  - 12087
DO  - 10.1021/acs.analchem.5b02801
ER  - 

@article{
author = "Habtamu, Henok B. and Sentić, Milica and Silvestrini, Morena and De Leo, Luigina and Not, Tarcisio and Arbault, Stephane and Manojlović, Dragan D. and Šojić, Nešo and Ugo, Paolo",
year = "2015",
abstract = "We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.",
publisher = "Amer Chemical Soc, Washington",
journal = "Analytical Chemistry",
title = "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles",
volume = "87",
number = "24",
pages = "12080-12087",
doi = "10.1021/acs.analchem.5b02801"
}

Habtamu, H. B., Sentić, M., Silvestrini, M., De Leo, L., Not, T., Arbault, S., Manojlović, D. D., Šojić, N.,& Ugo, P.. (2015). A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry
Amer Chemical Soc, Washington., 87(24), 12080-12087.
https://doi.org/10.1021/acs.analchem.5b02801

Habtamu HB, Sentić M, Silvestrini M, De Leo L, Not T, Arbault S, Manojlović DD, Šojić N, Ugo P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry. 2015;87(24):12080-12087.
doi:10.1021/acs.analchem.5b02801 .

Habtamu, Henok B., Sentić, Milica, Silvestrini, Morena, De Leo, Luigina, Not, Tarcisio, Arbault, Stephane, Manojlović, Dragan D., Šojić, Nešo, Ugo, Paolo, "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles" in Analytical Chemistry, 87, no. 24 (2015):12080-12087,
https://doi.org/10.1021/acs.analchem.5b02801 . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Arbault, Stephane
AU  - Bouffier, Laurent
AU  - Manojlović, Dragan D.
AU  - Kuhn, Alexander
AU  - Šojić, Nešo
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1737
AB  - Among luminescence techniques, electrogenerated chemiluminescence (ECL) provides a unique level of manipulation of the luminescent process by controlling the electrochemical trigger. Despite its attractiveness, ECL is by essence a 2D process where light emission is strictly confined to the electrode surface. To overcome this intrinsic limitation, we added a new spatial dimension to the ECL process by generating 3D ECL at the level of millions of micro-emitters dispersed in solution. Each single object is addressed remotely by bipolar electrochemistry and they generate collectively the luminescence in the bulk. Therefore, the entire volume of the solution produces light. To illustrate the generality of this concept, we extended it to a suspension of multi-walled carbon nanotubes where each one acts as an individual ECL nano-emitter. This approach enables a change of paradigm by switching from a surface-limited process to 3D electrogenerated light emission.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Science
T1  - 3D electrogenerated chemiluminescence: from surface-confined reactions to bulk emission
VL  - 6
IS  - 8
SP  - 4433
EP  - 4437
DO  - 10.1039/c5sc01530h
ER  - 

@article{
author = "Sentić, Milica and Arbault, Stephane and Bouffier, Laurent and Manojlović, Dragan D. and Kuhn, Alexander and Šojić, Nešo",
year = "2015",
abstract = "Among luminescence techniques, electrogenerated chemiluminescence (ECL) provides a unique level of manipulation of the luminescent process by controlling the electrochemical trigger. Despite its attractiveness, ECL is by essence a 2D process where light emission is strictly confined to the electrode surface. To overcome this intrinsic limitation, we added a new spatial dimension to the ECL process by generating 3D ECL at the level of millions of micro-emitters dispersed in solution. Each single object is addressed remotely by bipolar electrochemistry and they generate collectively the luminescence in the bulk. Therefore, the entire volume of the solution produces light. To illustrate the generality of this concept, we extended it to a suspension of multi-walled carbon nanotubes where each one acts as an individual ECL nano-emitter. This approach enables a change of paradigm by switching from a surface-limited process to 3D electrogenerated light emission.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Science",
title = "3D electrogenerated chemiluminescence: from surface-confined reactions to bulk emission",
volume = "6",
number = "8",
pages = "4433-4437",
doi = "10.1039/c5sc01530h"
}

Sentić, M., Arbault, S., Bouffier, L., Manojlović, D. D., Kuhn, A.,& Šojić, N.. (2015). 3D electrogenerated chemiluminescence: from surface-confined reactions to bulk emission. in Chemical Science
Royal Soc Chemistry, Cambridge., 6(8), 4433-4437.
https://doi.org/10.1039/c5sc01530h

Sentić M, Arbault S, Bouffier L, Manojlović DD, Kuhn A, Šojić N. 3D electrogenerated chemiluminescence: from surface-confined reactions to bulk emission. in Chemical Science. 2015;6(8):4433-4437.
doi:10.1039/c5sc01530h .

Sentić, Milica, Arbault, Stephane, Bouffier, Laurent, Manojlović, Dragan D., Kuhn, Alexander, Šojić, Nešo, "3D electrogenerated chemiluminescence: from surface-confined reactions to bulk emission" in Chemical Science, 6, no. 8 (2015):4433-4437,
https://doi.org/10.1039/c5sc01530h . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Milutinovic, Milena
AU  - Kanoufi, Frederic
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Šojić, Nešo
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1779
AB  - The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Science
T1  - Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays
VL  - 5
IS  - 6
SP  - 2568
EP  - 2572
DO  - 10.1039/c4sc00312h
ER  - 

@article{
author = "Sentić, Milica and Milutinovic, Milena and Kanoufi, Frederic and Manojlović, Dragan D. and Arbault, Stephane and Šojić, Nešo",
year = "2014",
abstract = "The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Science",
title = "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays",
volume = "5",
number = "6",
pages = "2568-2572",
doi = "10.1039/c4sc00312h"
}

Sentić, M., Milutinovic, M., Kanoufi, F., Manojlović, D. D., Arbault, S.,& Šojić, N.. (2014). Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science
Royal Soc Chemistry, Cambridge., 5(6), 2568-2572.
https://doi.org/10.1039/c4sc00312h

Sentić M, Milutinovic M, Kanoufi F, Manojlović DD, Arbault S, Šojić N. Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays. in Chemical Science. 2014;5(6):2568-2572.
doi:10.1039/c4sc00312h .

Sentić, Milica, Milutinovic, Milena, Kanoufi, Frederic, Manojlović, Dragan D., Arbault, Stephane, Šojić, Nešo, "Mapping electrogenerated chemiluminescence reactivity in space: mechanistic insight into model systems used in immunoassays" in Chemical Science, 5, no. 6 (2014):2568-2572,
https://doi.org/10.1039/c4sc00312h . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Arbault, Stephane
AU  - Goudeau, Bertrand
AU  - Manojlović, Dragan D.
AU  - Kuhn, Alexander
AU  - Bouffier, Laurent
AU  - Šojić, Nešo
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1834
AB  - An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is reported for enzymatic glucose sensing. The chemomechanical motion is induced by localized hydrogen bubble generation. The concomitant oxidation of the luminophore and of the enzymatically-produced NADH leads to ECL emission with a direct glucose-dependent light intensity. We demonstrate herein the local sensing and reporting of glucose in a concentration gradient explored by the ECL swimmer. Such a dynamic sensing approach combines in a synergetic way the wireless propulsion with the enzymatic selectivity using ECL as a readout method at the level of moving objects.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Communications
T1  - Electrochemiluminescent swimmers for dynamic enzymatic sensing
VL  - 50
IS  - 71
SP  - 10202
EP  - 10205
DO  - 10.1039/c4cc04105d
ER  - 

@article{
author = "Sentić, Milica and Arbault, Stephane and Goudeau, Bertrand and Manojlović, Dragan D. and Kuhn, Alexander and Bouffier, Laurent and Šojić, Nešo",
year = "2014",
abstract = "An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is reported for enzymatic glucose sensing. The chemomechanical motion is induced by localized hydrogen bubble generation. The concomitant oxidation of the luminophore and of the enzymatically-produced NADH leads to ECL emission with a direct glucose-dependent light intensity. We demonstrate herein the local sensing and reporting of glucose in a concentration gradient explored by the ECL swimmer. Such a dynamic sensing approach combines in a synergetic way the wireless propulsion with the enzymatic selectivity using ECL as a readout method at the level of moving objects.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Communications",
title = "Electrochemiluminescent swimmers for dynamic enzymatic sensing",
volume = "50",
number = "71",
pages = "10202-10205",
doi = "10.1039/c4cc04105d"
}

Sentić, M., Arbault, S., Goudeau, B., Manojlović, D. D., Kuhn, A., Bouffier, L.,& Šojić, N.. (2014). Electrochemiluminescent swimmers for dynamic enzymatic sensing. in Chemical Communications
Royal Soc Chemistry, Cambridge., 50(71), 10202-10205.
https://doi.org/10.1039/c4cc04105d

Sentić M, Arbault S, Goudeau B, Manojlović DD, Kuhn A, Bouffier L, Šojić N. Electrochemiluminescent swimmers for dynamic enzymatic sensing. in Chemical Communications. 2014;50(71):10202-10205.
doi:10.1039/c4cc04105d .

Sentić, Milica, Arbault, Stephane, Goudeau, Bertrand, Manojlović, Dragan D., Kuhn, Alexander, Bouffier, Laurent, Šojić, Nešo, "Electrochemiluminescent swimmers for dynamic enzymatic sensing" in Chemical Communications, 50, no. 71 (2014):10202-10205,
https://doi.org/10.1039/c4cc04105d . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Loget, Gabriel
AU  - Manojlović, Dragan D.
AU  - Kuhn, Alexander
AU  - Šojić, Nešo
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1549
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Angewandte Chemie (International Edition)
T1  - Light-Emitting Electrochemical "Swimmers"
VL  - 51
IS  - 45
SP  - 11284
EP  - 11288
DO  - 10.1002/anie.201206227
ER  - 

@article{
author = "Sentić, Milica and Loget, Gabriel and Manojlović, Dragan D. and Kuhn, Alexander and Šojić, Nešo",
year = "2012",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Angewandte Chemie (International Edition)",
title = "Light-Emitting Electrochemical "Swimmers"",
volume = "51",
number = "45",
pages = "11284-11288",
doi = "10.1002/anie.201206227"
}

Sentić, M., Loget, G., Manojlović, D. D., Kuhn, A.,& Šojić, N.. (2012). Light-Emitting Electrochemical "Swimmers". in Angewandte Chemie (International Edition)
Wiley-V C H Verlag Gmbh, Weinheim., 51(45), 11284-11288.
https://doi.org/10.1002/anie.201206227

Sentić M, Loget G, Manojlović DD, Kuhn A, Šojić N. Light-Emitting Electrochemical "Swimmers". in Angewandte Chemie (International Edition). 2012;51(45):11284-11288.
doi:10.1002/anie.201206227 .

Sentić, Milica, Loget, Gabriel, Manojlović, Dragan D., Kuhn, Alexander, Šojić, Nešo, "Light-Emitting Electrochemical "Swimmers"" in Angewandte Chemie (International Edition), 51, no. 45 (2012):11284-11288,
https://doi.org/10.1002/anie.201206227 . .

TY  - JOUR
AU  - Milutinovic, Milena
AU  - Sallard, Sebastien
AU  - Manojlović, Dragan D.
AU  - Mano, Nicolas
AU  - Šojić, Nešo
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1203
AB  - In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Sa, Lausanne
T2  - Bioelectrochemistry
T1  - Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel
VL  - 82
IS  - 1
SP  - 63
EP  - 68
DO  - 10.1016/j.bioelechem.2011.05.005
ER  - 

@article{
author = "Milutinovic, Milena and Sallard, Sebastien and Manojlović, Dragan D. and Mano, Nicolas and Šojić, Nešo",
year = "2011",
abstract = "In this work, we report a new sensing approach based on electrogenerated chemiluminescence (ECL) in an electrodeposited redox hydrogel using glucose dehydrogenase as a model system. The ECL-hydrogel films were electrodeposited by potential cycling of a PBS solution containing [poly(4-vinylpyridine)Ru(2,2'-bipyridine)(2) Cl-](+/2+). The film was easily prepared in a rapid, reproducible and well-controlled one-step procedure. The deposited hydrogel film is permeable to water-soluble chemicals and biochemicals, like enzyme substrates and coenzymes. Electrochemistry and ECL of NADH were studied at the level of the hydrogel film. Results indicate that ECL emission occurs at a relatively low anodic potential compared to the classical Ru(bipy)(3)(2+) complex. This is an important advantage since the measurements performed with the ECL hydrogel are thus less sensitive to interfering species. An ECL oxidative-reductive mechanism is presented for the ECL-hydrogel. Then we showed that the intensity of the ECL of NADH produced by the enzymatic activity varies with the enzyme substrate concentration. Such sensing approach combines enzymatic selectivity with the ECL advantages at low oxidation potential. (C) 2011 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Bioelectrochemistry",
title = "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel",
volume = "82",
number = "1",
pages = "63-68",
doi = "10.1016/j.bioelechem.2011.05.005"
}

Milutinovic, M., Sallard, S., Manojlović, D. D., Mano, N.,& Šojić, N.. (2011). Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry
Elsevier Science Sa, Lausanne., 82(1), 63-68.
https://doi.org/10.1016/j.bioelechem.2011.05.005

Milutinovic M, Sallard S, Manojlović DD, Mano N, Šojić N. Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel. in Bioelectrochemistry. 2011;82(1):63-68.
doi:10.1016/j.bioelechem.2011.05.005 .

Milutinovic, Milena, Sallard, Sebastien, Manojlović, Dragan D., Mano, Nicolas, Šojić, Nešo, "Glucose sensing by electrogenerated chemiluminescence of glucose-dehydrogenase produced NADH on electrodeposited redox hydrogel" in Bioelectrochemistry, 82, no. 1 (2011):63-68,
https://doi.org/10.1016/j.bioelechem.2011.05.005 . .

TY  - JOUR
AU  - Milutinovic, Milena
AU  - Suraniti, Emmanuel
AU  - Studer, Vincent
AU  - Mano, Nicolas
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1185
AB  - Photoinitiated polymerisation is efficiently and rapidly carried out to immobilise ultrathin electrochemiluminescent redox hydrogel films. Microscale patterns are fabricated on an electrode surface by a simple photolithographic procedure and revealed by ECL imaging.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Communications
T1  - Photopatterning of ultrathin electrochemiluminescent redox hydrogel films
VL  - 47
IS  - 32
SP  - 9125
EP  - 9127
DO  - 10.1039/c1cc12724a
ER  - 

@article{
author = "Milutinovic, Milena and Suraniti, Emmanuel and Studer, Vincent and Mano, Nicolas and Manojlović, Dragan D. and Šojić, Nešo",
year = "2011",
abstract = "Photoinitiated polymerisation is efficiently and rapidly carried out to immobilise ultrathin electrochemiluminescent redox hydrogel films. Microscale patterns are fabricated on an electrode surface by a simple photolithographic procedure and revealed by ECL imaging.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Communications",
title = "Photopatterning of ultrathin electrochemiluminescent redox hydrogel films",
volume = "47",
number = "32",
pages = "9125-9127",
doi = "10.1039/c1cc12724a"
}

Milutinovic, M., Suraniti, E., Studer, V., Mano, N., Manojlović, D. D.,& Šojić, N.. (2011). Photopatterning of ultrathin electrochemiluminescent redox hydrogel films. in Chemical Communications
Royal Soc Chemistry, Cambridge., 47(32), 9125-9127.
https://doi.org/10.1039/c1cc12724a

Milutinovic M, Suraniti E, Studer V, Mano N, Manojlović DD, Šojić N. Photopatterning of ultrathin electrochemiluminescent redox hydrogel films. in Chemical Communications. 2011;47(32):9125-9127.
doi:10.1039/c1cc12724a .

Milutinovic, Milena, Suraniti, Emmanuel, Studer, Vincent, Mano, Nicolas, Manojlović, Dragan D., Šojić, Nešo, "Photopatterning of ultrathin electrochemiluminescent redox hydrogel films" in Chemical Communications, 47, no. 32 (2011):9125-9127,
https://doi.org/10.1039/c1cc12724a . .