TY  - JOUR
AU  - Božić, Nataša
AU  - Šokarda-Slavić, Marinela
AU  - Gavrilović, Anja
AU  - Vujčić, Zoran
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1798
AB  - alpha-Amylase production by solid-state fermentation of different Bacillus sp. was studied previously on different fermentation media. However, no study has been reported on the influence of selected media on expression of desired amylase isoforms such as raw-starch-digesting amylase (RSDA). In this paper, the influence of different inexpensive and available agro-resources as solid media (corn, wheat and triticale) on alpha-amylase isoform induction from three wild-type Bacillus sp., selected among one hundred strains tested, namely 9B, 12B and 24A was investigated. For all three strains, tested amylases were detected in the multiple forms; however, number and intensity of each form differed depending on the solid media used for growth. To determine which isoform from Bacillus sp. 12B was RSDA, the suspected isoform was purified. The optimum pH for the purified alpha-amylase isoform was 6.0-8.0, while the optimum temperature was 60-90 A degrees C. Isoform was considerably thermostable and Ca2+-independent, and actually the only alpha-amylase active towards raw starch. Purification and characterization of RSDA showed that not all of the solid media tested induced RSDA. From an economic point of view, it might be significant to obtain pure isoenzyme for potential use in the raw-starch hydrolysis, since it was 5 times more efficient in raw corn starch hydrolysis than the crude amylase preparation.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Production of raw-starch-digesting alpha-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization
VL  - 37
IS  - 7
SP  - 1353
EP  - 1360
DO  - 10.1007/s00449-013-1105-1
ER  - 

@article{
author = "Božić, Nataša and Šokarda-Slavić, Marinela and Gavrilović, Anja and Vujčić, Zoran",
year = "2014",
abstract = "alpha-Amylase production by solid-state fermentation of different Bacillus sp. was studied previously on different fermentation media. However, no study has been reported on the influence of selected media on expression of desired amylase isoforms such as raw-starch-digesting amylase (RSDA). In this paper, the influence of different inexpensive and available agro-resources as solid media (corn, wheat and triticale) on alpha-amylase isoform induction from three wild-type Bacillus sp., selected among one hundred strains tested, namely 9B, 12B and 24A was investigated. For all three strains, tested amylases were detected in the multiple forms; however, number and intensity of each form differed depending on the solid media used for growth. To determine which isoform from Bacillus sp. 12B was RSDA, the suspected isoform was purified. The optimum pH for the purified alpha-amylase isoform was 6.0-8.0, while the optimum temperature was 60-90 A degrees C. Isoform was considerably thermostable and Ca2+-independent, and actually the only alpha-amylase active towards raw starch. Purification and characterization of RSDA showed that not all of the solid media tested induced RSDA. From an economic point of view, it might be significant to obtain pure isoenzyme for potential use in the raw-starch hydrolysis, since it was 5 times more efficient in raw corn starch hydrolysis than the crude amylase preparation.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Production of raw-starch-digesting alpha-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization",
volume = "37",
number = "7",
pages = "1353-1360",
doi = "10.1007/s00449-013-1105-1"
}

Božić, N., Šokarda-Slavić, M., Gavrilović, A.,& Vujčić, Z.. (2014). Production of raw-starch-digesting alpha-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization. in Bioprocess and Biosystems Engineering
Springer, New York., 37(7), 1353-1360.
https://doi.org/10.1007/s00449-013-1105-1

Božić N, Šokarda-Slavić M, Gavrilović A, Vujčić Z. Production of raw-starch-digesting alpha-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization. in Bioprocess and Biosystems Engineering. 2014;37(7):1353-1360.
doi:10.1007/s00449-013-1105-1 .

Božić, Nataša, Šokarda-Slavić, Marinela, Gavrilović, Anja, Vujčić, Zoran, "Production of raw-starch-digesting alpha-amylase isoform from Bacillus sp under solid-state fermentation and biochemical characterization" in Bioprocess and Biosystems Engineering, 37, no. 7 (2014):1353-1360,
https://doi.org/10.1007/s00449-013-1105-1 . .

TY  - JOUR
AU  - Šokarda-Slavić, Marinela
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1850
AB  - Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Growth Temperature of Different Local Isolates of Bacillus Sp in the Solid State Affects Production of Raw Starch Digesting Amylases
VL  - 66
IS  - 2
SP  - 483
EP  - 490
DO  - 10.2298/ABS1402483S
ER  - 

@article{
author = "Šokarda-Slavić, Marinela and Božić, Nataša and Vujčić, Zoran",
year = "2014",
abstract = "Natural amylase producers, wild type strains of Bacillus sp., were isolated from different regions of Serbia. Strains with the highest amylase activity based on the starch-agar plate test were grown on solid-state fermentation (SSF) on triticale. The influence of the substrate and different cultivation temperature (28 and 37 degrees C) on the production of amylase was examined. The tested strains produced alpha-amylases when grown on triticale grains both at 28 and at 37 degrees C, but the activity of amylases and the number and intensity of the produced isoforms were different. Significant hydrolysis of raw cornstarch was obtained with the Bacillus sp. strains 2B, 5B, 18 and 24B. The produced alpha-amylases hydrolyzed raw cornstarch at a temperature below the temperature of gelatinization, but the ability for hydrolysis was not directly related to the total enzyme activity, suggesting that only certain isoforms are involved in the hydrolysis.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Growth Temperature of Different Local Isolates of Bacillus Sp in the Solid State Affects Production of Raw Starch Digesting Amylases",
volume = "66",
number = "2",
pages = "483-490",
doi = "10.2298/ABS1402483S"
}

Šokarda-Slavić, M., Božić, N.,& Vujčić, Z.. (2014). Growth Temperature of Different Local Isolates of Bacillus Sp in the Solid State Affects Production of Raw Starch Digesting Amylases. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 66(2), 483-490.
https://doi.org/10.2298/ABS1402483S

Šokarda-Slavić M, Božić N, Vujčić Z. Growth Temperature of Different Local Isolates of Bacillus Sp in the Solid State Affects Production of Raw Starch Digesting Amylases. in Archives of biological sciences. 2014;66(2):483-490.
doi:10.2298/ABS1402483S .

Šokarda-Slavić, Marinela, Božić, Nataša, Vujčić, Zoran, "Growth Temperature of Different Local Isolates of Bacillus Sp in the Solid State Affects Production of Raw Starch Digesting Amylases" in Archives of biological sciences, 66, no. 2 (2014):483-490,
https://doi.org/10.2298/ABS1402483S . .