Biotechnology in vitro - crop, medicinal and endangered plant species

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Biotechnology in vitro - crop, medicinal and endangered plant species (en)
Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (sr)
Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (sr_RS)
Authors

Publications

Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base

Petric, Marija; Subotic, Angelina; Jevremovic, Sladana; Trifunović-Momcilov, Milana; Tadić, Vojin; Grujić, Marica; Vujčić, Zoran

(Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd, 2017)

TY  - JOUR
AU  - Petric, Marija
AU  - Subotic, Angelina
AU  - Jevremovic, Sladana
AU  - Trifunović-Momcilov, Milana
AU  - Tadić, Vojin
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2543
AB  - The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base
VL  - 69
IS  - 4
SP  - 619
EP  - 625
DO  - 10.2298/ABS161130007P
UR  - Kon_3359
ER  - 
@article{
author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
year = "2017",
abstract = "The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base",
volume = "69",
number = "4",
pages = "619-625",
doi = "10.2298/ABS161130007P",
url = "Kon_3359"
}
Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2017). Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 69(4), 619-625.
https://doi.org/10.2298/ABS161130007P
Kon_3359
Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences. 2017;69(4):619-625.
doi:10.2298/ABS161130007P
Kon_3359 .
Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base" in Archives of biological sciences, 69, no. 4 (2017):619-625,
https://doi.org/10.2298/ABS161130007P .,
Kon_3359 .

Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture

Petric, Marija; Subotic, Angelina; Jevremovic, Sladana; Trifunović-Momcilov, Milana; Tadić, Vojin; Grujić, Marica; Vujčić, Zoran

(Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux, 2015)

TY  - JOUR
AU  - Petric, Marija
AU  - Subotic, Angelina
AU  - Jevremovic, Sladana
AU  - Trifunović-Momcilov, Milana
AU  - Tadić, Vojin
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2032
AB  - Morphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 degrees C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1 mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1 mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process. 2015 Academie des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Comptes Rendus Biologies
T1  - Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture
VL  - 338
IS  - 12
SP  - 793
EP  - 802
DO  - 10.1016/j.crvi.2015.08.002
UR  - Kon_2987
ER  - 
@article{
author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "Morphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 degrees C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1 mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1 mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process. 2015 Academie des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Comptes Rendus Biologies",
title = "Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture",
volume = "338",
number = "12",
pages = "793-802",
doi = "10.1016/j.crvi.2015.08.002",
url = "Kon_2987"
}
Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2015). Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 338(12), 793-802.
https://doi.org/10.1016/j.crvi.2015.08.002
Kon_2987
Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies. 2015;338(12):793-802.
doi:10.1016/j.crvi.2015.08.002
Kon_2987 .
Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture" in Comptes Rendus Biologies, 338, no. 12 (2015):793-802,
https://doi.org/10.1016/j.crvi.2015.08.002 .,
Kon_2987 .
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Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia

Beatovic, Damir; Krstic-Milosevic, Dijana; Trifunović, Snežana S.; Siljegovic, Jovana; Glamočlija, Jasmina; Ristic, Mihailo; Jelacic, Slavica

(Acg Publications, Gebze-Kocaeli, 2015)

TY  - JOUR
AU  - Beatovic, Damir
AU  - Krstic-Milosevic, Dijana
AU  - Trifunović, Snežana S.
AU  - Siljegovic, Jovana
AU  - Glamočlija, Jasmina
AU  - Ristic, Mihailo
AU  - Jelacic, Slavica
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1672
AB  - This study evaluated the chemical composition, antioxidant and antimicrobial activities of the essential oils of twelve Ocimum basilicum L. cultivars grown in Serbia. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The oil yields ranged from 0.65 to 1.90 %. A total of 75 compounds were identified as constituents of analyzed essential oils. GC/MS analyses revealed that a majority of the examined basil cultivars belonged to the "linalool chemotype". The results of the DPPH assay showed a very high antioxidant capacity of the basil oils, which was especially high for Blue Spice cultivar (IC50 = 0.03 mu g/mL). Significant antimicrobial activity was shown for all the tested oils. Essential oil of Compact cultivar was the most active against the bacterium Micrococcus flavus, with an MIC value of 0.009 mu g/mL while Osmin cultivar exhibited the strongest antifungal activity with MIC values ranging from 0.08-1.07 mu g/mL. All the examined essential oils showed a 10-to 100-fold greater ability to inhibit fungal growth compared to commercial antifungal agents.
PB  - Acg Publications, Gebze-Kocaeli
T2  - Records of Natural Products
T1  - Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia
VL  - 9
IS  - 1
SP  - 62
EP  - 75
UR  - Kon_2818
ER  - 
@article{
author = "Beatovic, Damir and Krstic-Milosevic, Dijana and Trifunović, Snežana S. and Siljegovic, Jovana and Glamočlija, Jasmina and Ristic, Mihailo and Jelacic, Slavica",
year = "2015",
abstract = "This study evaluated the chemical composition, antioxidant and antimicrobial activities of the essential oils of twelve Ocimum basilicum L. cultivars grown in Serbia. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection and GC-mass spectrometry. The oil yields ranged from 0.65 to 1.90 %. A total of 75 compounds were identified as constituents of analyzed essential oils. GC/MS analyses revealed that a majority of the examined basil cultivars belonged to the "linalool chemotype". The results of the DPPH assay showed a very high antioxidant capacity of the basil oils, which was especially high for Blue Spice cultivar (IC50 = 0.03 mu g/mL). Significant antimicrobial activity was shown for all the tested oils. Essential oil of Compact cultivar was the most active against the bacterium Micrococcus flavus, with an MIC value of 0.009 mu g/mL while Osmin cultivar exhibited the strongest antifungal activity with MIC values ranging from 0.08-1.07 mu g/mL. All the examined essential oils showed a 10-to 100-fold greater ability to inhibit fungal growth compared to commercial antifungal agents.",
publisher = "Acg Publications, Gebze-Kocaeli",
journal = "Records of Natural Products",
title = "Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia",
volume = "9",
number = "1",
pages = "62-75",
url = "Kon_2818"
}
Beatovic, D., Krstic-Milosevic, D., Trifunović, S. S., Siljegovic, J., Glamočlija, J., Ristic, M.,& Jelacic, S.. (2015). Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia. in Records of Natural Products
Acg Publications, Gebze-Kocaeli., 9(1), 62-75.
Kon_2818
Beatovic D, Krstic-Milosevic D, Trifunović SS, Siljegovic J, Glamočlija J, Ristic M, Jelacic S. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia. in Records of Natural Products. 2015;9(1):62-75.
Kon_2818 .
Beatovic, Damir, Krstic-Milosevic, Dijana, Trifunović, Snežana S., Siljegovic, Jovana, Glamočlija, Jasmina, Ristic, Mihailo, Jelacic, Slavica, "Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oils of Twelve Ocimum basilicum L. Cultivars Grown in Serbia" in Records of Natural Products, 9, no. 1 (2015):62-75,
Kon_2818 .
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Xanthone compounds in shoot cultures of Gentianella bulgarica

Jankovic, Teodora; Vinterhalter, Branka; Krstic-Milosevic, Dijana; Nikolic, Radomirka; Vinterhalter, Dragan; Milosavljević, Slobodan M.

(Springer Heidelberg, Heidelberg, 2011)

TY  - JOUR
AU  - Jankovic, Teodora
AU  - Vinterhalter, Branka
AU  - Krstic-Milosevic, Dijana
AU  - Nikolic, Radomirka
AU  - Vinterhalter, Dragan
AU  - Milosavljević, Slobodan M.
PY  - 2011
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1169
AB  - Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Xanthone compounds in shoot cultures of Gentianella bulgarica
VL  - 33
IS  - 4
SP  - 1515
EP  - 1520
DO  - 10.1007/s11738-010-0689-1
UR  - Kon_2191
ER  - 
@article{
author = "Jankovic, Teodora and Vinterhalter, Branka and Krstic-Milosevic, Dijana and Nikolic, Radomirka and Vinterhalter, Dragan and Milosavljević, Slobodan M.",
year = "2011",
abstract = "Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Xanthone compounds in shoot cultures of Gentianella bulgarica",
volume = "33",
number = "4",
pages = "1515-1520",
doi = "10.1007/s11738-010-0689-1",
url = "Kon_2191"
}
Jankovic, T., Vinterhalter, B., Krstic-Milosevic, D., Nikolic, R., Vinterhalter, D.,& Milosavljević, S. M.. (2011). Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 33(4), 1515-1520.
https://doi.org/10.1007/s11738-010-0689-1
Kon_2191
Jankovic T, Vinterhalter B, Krstic-Milosevic D, Nikolic R, Vinterhalter D, Milosavljević SM. Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum. 2011;33(4):1515-1520.
doi:10.1007/s11738-010-0689-1
Kon_2191 .
Jankovic, Teodora, Vinterhalter, Branka, Krstic-Milosevic, Dijana, Nikolic, Radomirka, Vinterhalter, Dragan, Milosavljević, Slobodan M., "Xanthone compounds in shoot cultures of Gentianella bulgarica" in Acta Physiologiae Plantarum, 33, no. 4 (2011):1515-1520,
https://doi.org/10.1007/s11738-010-0689-1 .,
Kon_2191 .
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