TY  - JOUR
AU  - Stanković, M.
AU  - Bartolić, D.
AU  - Šikoparija, Branko
AU  - Spasojević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3115
AB  - It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.
T2  - Journal of Applied Spectroscopy
T1  - Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis
VL  - 86
IS  - 2
SP  - 256
EP  - 263
DO  - 10.1007/s10812-019-00809-1
ER  - 

@article{
author = "Stanković, M. and Bartolić, D. and Šikoparija, Branko and Spasojević, Dragica and Mutavdžić, Dragosav and Natić, Maja and Radotić, Ksenija",
year = "2019",
abstract = "It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.",
journal = "Journal of Applied Spectroscopy",
title = "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis",
volume = "86",
number = "2",
pages = "256-263",
doi = "10.1007/s10812-019-00809-1"
}

Stanković, M., Bartolić, D., Šikoparija, B., Spasojević, D., Mutavdžić, D., Natić, M.,& Radotić, K.. (2019). Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. in Journal of Applied Spectroscopy, 86(2), 256-263.
https://doi.org/10.1007/s10812-019-00809-1

Stanković M, Bartolić D, Šikoparija B, Spasojević D, Mutavdžić D, Natić M, Radotić K. Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. in Journal of Applied Spectroscopy. 2019;86(2):256-263.
doi:10.1007/s10812-019-00809-1 .

Stanković, M., Bartolić, D., Šikoparija, Branko, Spasojević, Dragica, Mutavdžić, Dragosav, Natić, Maja, Radotić, Ksenija, "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis" in Journal of Applied Spectroscopy, 86, no. 2 (2019):256-263,
https://doi.org/10.1007/s10812-019-00809-1 . .

TY  - JOUR
AU  - Gašić, Uroš M.
AU  - Šikoparija, Branko
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Natić, Maja
AU  - Tešić, Živoslav Lj.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1854
AB  - Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
PB  - Aoac Int, Gaithersburg
T2  - Journal of AOAC International / Association of Analytical Communities
T1  - Phytochemical Fingerprints of Lime Honey Collected in Serbia
VL  - 97
IS  - 5
SP  - 1259
EP  - 1267
DO  - 10.5740/jaoacint.SGEGasic
ER  - 

@article{
author = "Gašić, Uroš M. and Šikoparija, Branko and Tosti, Tomislav and Trifković, Jelena and Milojković-Opsenica, Dušanka and Natić, Maja and Tešić, Živoslav Lj.",
year = "2014",
abstract = "Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC-OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.",
publisher = "Aoac Int, Gaithersburg",
journal = "Journal of AOAC International / Association of Analytical Communities",
title = "Phytochemical Fingerprints of Lime Honey Collected in Serbia",
volume = "97",
number = "5",
pages = "1259-1267",
doi = "10.5740/jaoacint.SGEGasic"
}

Gašić, U. M., Šikoparija, B., Tosti, T., Trifković, J., Milojković-Opsenica, D., Natić, M.,& Tešić, Ž. Lj.. (2014). Phytochemical Fingerprints of Lime Honey Collected in Serbia. in Journal of AOAC International / Association of Analytical Communities
Aoac Int, Gaithersburg., 97(5), 1259-1267.
https://doi.org/10.5740/jaoacint.SGEGasic

Gašić UM, Šikoparija B, Tosti T, Trifković J, Milojković-Opsenica D, Natić M, Tešić ŽL. Phytochemical Fingerprints of Lime Honey Collected in Serbia. in Journal of AOAC International / Association of Analytical Communities. 2014;97(5):1259-1267.
doi:10.5740/jaoacint.SGEGasic .

Gašić, Uroš M., Šikoparija, Branko, Tosti, Tomislav, Trifković, Jelena, Milojković-Opsenica, Dušanka, Natić, Maja, Tešić, Živoslav Lj., "Phytochemical Fingerprints of Lime Honey Collected in Serbia" in Journal of AOAC International / Association of Analytical Communities, 97, no. 5 (2014):1259-1267,
https://doi.org/10.5740/jaoacint.SGEGasic . .