International Centre for Genetic Engineering and Biotechnology (ICGEB) [CRP/YUG11-02]

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International Centre for Genetic Engineering and Biotechnology (ICGEB) [CRP/YUG11-02]

Authors

Publications

Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase

Šokarda-Slavić, Marinela; Pešić, Milja; Vujčić, Zoran; Božić, Nataša

(Springer, New York, 2016) 

TY  - JOUR
AU  - Šokarda-Slavić, Marinela
AU  - Pešić, Milja
AU  - Vujčić, Zoran
AU  - Božić, Nataša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2061
AB  - alpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase
VL  - 100
IS  - 6
SP  - 2709
EP  - 2719
DO  - 10.1007/s00253-015-7101-4
ER  - 
@article{
author = "Šokarda-Slavić, Marinela and Pešić, Milja and Vujčić, Zoran and Božić, Nataša",
year = "2016",
abstract = "alpha-Amylase from Bacillus licheniformis ATCC 9945a (BliAmy) was proven to be very efficient in hydrolysis of granular starch below the temperature of gelatinization. By applying two-stage feeding strategy to achieve high-cell-density cultivation of Escherichia coli and extracellular production of BliAmy, total of 250.5 U/mL (i.e. 0.7 g/L) of enzyme was obtained. Thermostability of amylase was exploited to simplify purification. The hydrolysis of concentrated raw starch was optimized using response surface methodology. Regardless of raw starch concentration tested (20, 25, 30 %), BliAmy was very effective, achieving the final hydrolysis degree of 91 % for the hydrolysis of 30 % starch suspension after 24 h. The major A-type crystalline structure and amorphous domains of the starch granule were degraded at the same rates, while amylose-lipid complexes were not degraded. BliAmy presents interesting performances on highly concentrated solid starch and could be of value for starch-consuming industries while response surface methodology (RSM) could be efficiently applied for the optimization of the hydrolysis.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase",
volume = "100",
number = "6",
pages = "2709-2719",
doi = "10.1007/s00253-015-7101-4"
}
Šokarda-Slavić, M., Pešić, M., Vujčić, Z.,& Božić, N.. (2016). Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase. in Applied Microbiology and Biotechnology
Springer, New York., 100(6), 2709-2719.
https://doi.org/10.1007/s00253-015-7101-4
Šokarda-Slavić M, Pešić M, Vujčić Z, Božić N. Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase. in Applied Microbiology and Biotechnology. 2016;100(6):2709-2719.
doi:10.1007/s00253-015-7101-4 .
Šokarda-Slavić, Marinela, Pešić, Milja, Vujčić, Zoran, Božić, Nataša, "Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a alpha-amylase" in Applied Microbiology and Biotechnology, 100, no. 6 (2016):2709-2719,
https://doi.org/10.1007/s00253-015-7101-4 . .
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Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4

Šokarda-Slavić, Marinela; Pešić, Milja; Vujčić, Zoran; Božić, Nataša

(Springer, New York, 2016) 

TY  - DATA
AU  - Šokarda-Slavić, Marinela
AU  - Pešić, Milja
AU  - Vujčić, Zoran
AU  - Božić, Nataša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3588
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn  Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3588
ER  - 
@misc{
author = "Šokarda-Slavić, Marinela and Pešić, Milja and Vujčić, Zoran and Božić, Nataša",
year = "2016",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn  Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3588"
}
Šokarda-Slavić, M., Pešić, M., Vujčić, Z.,& Božić, N.. (2016). Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn  Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4. in Applied Microbiology and Biotechnology
Springer, New York..
https://hdl.handle.net/21.15107/rcub_cherry_3588
Šokarda-Slavić M, Pešić M, Vujčić Z, Božić N. Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn  Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4. in Applied Microbiology and Biotechnology. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3588 .
Šokarda-Slavić, Marinela, Pešić, Milja, Vujčić, Zoran, Božić, Nataša, "Supplementary material for the article: Šokarda Slavić, M.; Pešić, M.; Vujčić, Z.; Božić, N. Overcoming Hydrolysis of Raw Corn  Starch under Industrial Conditions with Bacillus Licheniformis ATCC 9945a α-Amylase. Applied Microbiology and Biotechnology 2016, 100 (6), 2709–2719. https://doi.org/10.1007/s00253-015-7101-4" in Applied Microbiology and Biotechnology (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3588 .