TY  - JOUR
AU  - Djurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Kekez, Branka
AU  - Stefanović Kojić, Jovana R.
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav M.
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2538
AB  - Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
VL  - 104
SP  - 311
EP  - 321
DO  - 10.1016/j.ijbiomac.2017.06.034
ER  - 

@article{
author = "Djurić, Aleksandra and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Kekez, Branka and Stefanović Kojić, Jovana R. and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav M. and Beškoski, Vladimir",
year = "2017",
abstract = "Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan",
volume = "104",
pages = "311-321",
doi = "10.1016/j.ijbiomac.2017.06.034"
}

Djurić, A., Gojgić-Cvijović, G. D., Jakovljević, D. M., Kekez, B., Stefanović Kojić, J. R., Mattinen, M., Harju, I. E., Vrvić, M. M.,& Beškoski, V.. (2017). Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam., 104, 311-321.
https://doi.org/10.1016/j.ijbiomac.2017.06.034

Djurić A, Gojgić-Cvijović GD, Jakovljević DM, Kekez B, Stefanović Kojić JR, Mattinen M, Harju IE, Vrvić MM, Beškoski V. Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules. 2017;104:311-321.
doi:10.1016/j.ijbiomac.2017.06.034 .

Djurić, Aleksandra, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Kekez, Branka, Stefanović Kojić, Jovana R., Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav M., Beškoski, Vladimir, "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan" in International Journal of Biological Macromolecules, 104 (2017):311-321,
https://doi.org/10.1016/j.ijbiomac.2017.06.034 . .

TY  - DATA
AU  - Djurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Kekez, Branka
AU  - Stefanović Kojić, Jovana R.
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav M.
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2965
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2965
ER  - 

@misc{
author = "Djurić, Aleksandra and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Kekez, Branka and Stefanović Kojić, Jovana R. and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav M. and Beškoski, Vladimir",
year = "2017",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2965"
}

Djurić, A., Gojgić-Cvijović, G. D., Jakovljević, D. M., Kekez, B., Stefanović Kojić, J. R., Mattinen, M., Harju, I. E., Vrvić, M. M.,& Beškoski, V.. (2017). Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_2965

Djurić A, Gojgić-Cvijović GD, Jakovljević DM, Kekez B, Stefanović Kojić JR, Mattinen M, Harju IE, Vrvić MM, Beškoski V. Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034. in International Journal of Biological Macromolecules. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_2965 .

Djurić, Aleksandra, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Kekez, Branka, Stefanović Kojić, Jovana R., Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav M., Beškoski, Vladimir, "Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034" in International Journal of Biological Macromolecules (2017),
https://hdl.handle.net/21.15107/rcub_cherry_2965 .