TY  - JOUR
AU  - Jurica, Karlo
AU  - Brčić-Karačonji, Irena
AU  - Mikolić, Anja
AU  - Milojković-Opsenica, Dušanka
AU  - Benković, Vesna
AU  - Kopjar, Nevenka
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2194
AB  - Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.
PB  - Springer, Dordrecht
T2  - Cytotechnology
T1  - In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes
VL  - 70
IS  - 4
SP  - 1261
EP  - 1278
DO  - 10.1007/s10616-018-0218-4
ER  - 

@article{
author = "Jurica, Karlo and Brčić-Karačonji, Irena and Mikolić, Anja and Milojković-Opsenica, Dušanka and Benković, Vesna and Kopjar, Nevenka",
year = "2018",
abstract = "Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.",
publisher = "Springer, Dordrecht",
journal = "Cytotechnology",
title = "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes",
volume = "70",
number = "4",
pages = "1261-1278",
doi = "10.1007/s10616-018-0218-4"
}

Jurica, K., Brčić-Karačonji, I., Mikolić, A., Milojković-Opsenica, D., Benković, V.,& Kopjar, N.. (2018). In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology
Springer, Dordrecht., 70(4), 1261-1278.
https://doi.org/10.1007/s10616-018-0218-4

Jurica K, Brčić-Karačonji I, Mikolić A, Milojković-Opsenica D, Benković V, Kopjar N. In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes. in Cytotechnology. 2018;70(4):1261-1278.
doi:10.1007/s10616-018-0218-4 .

Jurica, Karlo, Brčić-Karačonji, Irena, Mikolić, Anja, Milojković-Opsenica, Dušanka, Benković, Vesna, Kopjar, Nevenka, "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes" in Cytotechnology, 70, no. 4 (2018):1261-1278,
https://doi.org/10.1007/s10616-018-0218-4 . .

TY  - JOUR
AU  - Jurica, Karlo
AU  - Brčić-Karačonji, Irena
AU  - Šegan, Sandra B.
AU  - Milojković-Opsenica, Dušanka
AU  - Kremer, Dario
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2009
AB  - The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2 gt 0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD lt 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry
VL  - 66
IS  - 3
SP  - 197
EP  - 202
DO  - 10.1515/aiht-2015-66-2696
ER  - 

@article{
author = "Jurica, Karlo and Brčić-Karačonji, Irena and Šegan, Sandra B. and Milojković-Opsenica, Dušanka and Kremer, Dario",
year = "2015",
abstract = "The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2 gt 0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD lt 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry",
volume = "66",
number = "3",
pages = "197-202",
doi = "10.1515/aiht-2015-66-2696"
}

Jurica, K., Brčić-Karačonji, I., Šegan, S. B., Milojković-Opsenica, D.,& Kremer, D.. (2015). Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv za higijenu rada i toksikologiju
Inst Medical Research & Occupational Health, Zagreb., 66(3), 197-202.
https://doi.org/10.1515/aiht-2015-66-2696

Jurica K, Brčić-Karačonji I, Šegan SB, Milojković-Opsenica D, Kremer D. Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv za higijenu rada i toksikologiju. 2015;66(3):197-202.
doi:10.1515/aiht-2015-66-2696 .

Jurica, Karlo, Brčić-Karačonji, Irena, Šegan, Sandra B., Milojković-Opsenica, Dušanka, Kremer, Dario, "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry" in Arhiv za higijenu rada i toksikologiju, 66, no. 3 (2015):197-202,
https://doi.org/10.1515/aiht-2015-66-2696 . .