Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry)

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info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//

Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200168 (Univerzitet u Beogradu, Hemijski fakultet) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200168 (Универзитет у Београду, Хемијски факултет) (sr)
Authors

Publications

Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481

Šegan, Sandra; Jevtić, Ivana; Tosti, Tomislav; Penjišević, Jelena; Šukalović, Vladimir; Kostić-Rajačić, Slađana; Milojković-Opsenica, Dušanka

TY  - DATA
AU  - Šegan, Sandra
AU  - Jevtić, Ivana
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić-Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5650
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481
VL  - 1211
SP  - 123481
ER  - 
@misc{
author = "Šegan, Sandra and Jevtić, Ivana and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić-Rajačić, Slađana and Milojković-Opsenica, Dušanka",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of thepiperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constantof fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-ophilicity, expressed as retention indices RM0 , b, and C0, as well as PC1, was determined and correlated with insilico values. Ionization constants were determined on the basis of the relationships between analyte’s retentionexpressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, weresubjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to providebasic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeationwas investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-lecular weight. In general, results of the present research corroborate well with previously determined anti-nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, anotherset of important parameters should be taken into account when designing new derivatives of C-3 substitutedfentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLCcan be considered as a valuable asset in the ligand-based drug design",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481",
volume = "1211",
pages = "123481"
}
Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D..Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B
Elsevier., 1211, 123481.
Šegan S, Jevtić I, Tosti T, Penjišević J, Šukalović V, Kostić-Rajačić S, Milojković-Opsenica D. Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481. in Journal of Chromatography B.1211:123481..
Šegan, Sandra, Jevtić, Ivana, Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić-Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Supplementary material for: Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B Elsevier., 1211, 123481. https://doi.org/10.1016/j.jchromb.2022.123481" in Journal of Chromatography B, 1211:123481.

Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184

Krstić, Đurđa D.; Ristivojević, Petar; Gašić, Uroš M.; Lazović, Mila; Fotirić Akšić, Milica M.; Milivojević, Jasminka; Morlock, Gertrud E.; Milojković-Opsenica, Dušanka; Trifković, Jelena

(Elsevier, 2023)

TY  - DATA
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5633
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184
VL  - 402
SP  - 134184
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5633
ER  - 
@misc{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticityas one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combinedwith multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivatedberry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible forthe classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and theirderivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds provedto be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184",
volume = "402",
pages = "134184",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5633"
}
Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D.,& Trifković, J.. (2023). Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry
Elsevier., 402, 134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633
Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica D, Trifković J. Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184. in Food Chemistry. 2023;402:134184.
https://hdl.handle.net/21.15107/rcub_cherry_5633 .
Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka, Trifković, Jelena, "Supplementary material for: Krstić, Đ. D., Ristivojević, P. M., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J. Đ.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry Elsevier., 402, 134184. https://doi.org/10.1016/j.foodchem.2022.134184" in Food Chemistry, 402 (2023):134184,
https://hdl.handle.net/21.15107/rcub_cherry_5633 .

Authenticity assessment of cultivated berries via phenolic profiles of seeds

Krstić, Đurđa D.; Ristivojević, Petar; Gašić, Uroš M.; Lazović, Mila; Fotirić Akšić, Milica M.; Milivojević, Jasminka; Morlock, Gertrud E.; Milojković-Opsenica, Dušanka M.; Trifković, Jelena

(Elsevier, 2023)

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Ristivojević, Petar
AU  - Gašić, Uroš M.
AU  - Lazović, Mila
AU  - Fotirić Akšić, Milica M.
AU  - Milivojević, Jasminka
AU  - Morlock, Gertrud E.
AU  - Milojković-Opsenica, Dušanka M.
AU  - Trifković, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5629
AB  - Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.
PB  - Elsevier
T2  - Food Chemistry
T1  - Authenticity assessment of cultivated berries via phenolic profiles of seeds
VL  - 402
SP  - 134184
DO  - 10.1016/j.foodchem.2022.134184
ER  - 
@article{
author = "Krstić, Đurđa D. and Ristivojević, Petar and Gašić, Uroš M. and Lazović, Mila and Fotirić Akšić, Milica M. and Milivojević, Jasminka and Morlock, Gertrud E. and Milojković-Opsenica, Dušanka M. and Trifković, Jelena",
year = "2023",
abstract = "Considering the health-benefits of berry fruits consumption and increased market demands for food authenticity
as one of the most important quality assurances, phenolic profiling by high-performance thin layer chroma-
tography and ultra-high-performance liquid chromatography hyphenated with mass spectrometry was combined
with multivariate analysis for phytochemical characterization and intercultivar discrimination of cultivated
berry seeds. The phenolic profiles of 45 berry seeds from nine genuine Serbian cultivated fruit species (straw-
berry, raspberry, blackberry, black currant, blueberry, gooseberry, cape gooseberry, chokeberry, and goji berry)
revealed a good differentiation according to botanical origin. In order to determine biomarkers responsible for
the classification, a total of 103 phenolic compounds were identified, including 53 phenolic acids and their
derivatives, 26 flavonoids and 24 glycosides. Biomarkers derived from the phenolic profile of berry seeds proved
to be a powerful tool in the authentication of botanical origin, and may be useful in detection of frauds in berry-
based seed-containing product.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Authenticity assessment of cultivated berries via phenolic profiles of seeds",
volume = "402",
pages = "134184",
doi = "10.1016/j.foodchem.2022.134184"
}
Krstić, Đ. D., Ristivojević, P., Gašić, U. M., Lazović, M., Fotirić Akšić, M. M., Milivojević, J., Morlock, G. E., Milojković-Opsenica, D. M.,& Trifković, J.. (2023). Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry
Elsevier., 402, 134184.
https://doi.org/10.1016/j.foodchem.2022.134184
Krstić ĐD, Ristivojević P, Gašić UM, Lazović M, Fotirić Akšić MM, Milivojević J, Morlock GE, Milojković-Opsenica DM, Trifković J. Authenticity assessment of cultivated berries via phenolic profiles of seeds. in Food Chemistry. 2023;402:134184.
doi:10.1016/j.foodchem.2022.134184 .
Krstić, Đurđa D., Ristivojević, Petar, Gašić, Uroš M., Lazović, Mila, Fotirić Akšić, Milica M., Milivojević, Jasminka, Morlock, Gertrud E., Milojković-Opsenica, Dušanka M., Trifković, Jelena, "Authenticity assessment of cultivated berries via phenolic profiles of seeds" in Food Chemistry, 402 (2023):134184,
https://doi.org/10.1016/j.foodchem.2022.134184 . .

INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity

Prodić, Ivana; Smiljanić, Katarina; Nagl, Christoph; Ballmer-Weber, Barbara; Hoffmann-Sommergruber, Karin; Ćirković-Veličković, Tanja

(MDPI, 2022)

TY  - JOUR
AU  - Prodić, Ivana
AU  - Smiljanić, Katarina
AU  - Nagl, Christoph
AU  - Ballmer-Weber, Barbara
AU  - Hoffmann-Sommergruber, Karin
AU  - Ćirković-Veličković, Tanja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5653
AB  - Most of the food allergens sensitized via the gastrointestinal tract resist thermal treatments and digestion, particularly digestion by pepsin. Roasted hazelnuts are more commonly consumed than raw ones. Since no studies have characterized gastric digestion protein fragments of raw and roasted hazelnuts nor their IgE binding properties, we compared these aspects of raw and roasted hazelnuts’ gastric digesta obtained by INFOGEST protocol. Their electrophoretically resolved profiles were probed with hazelnut allergic patients’ sera in 1D and 2D immunoblots. Electrophoretic profiles demonstrated pepsin digestion of all hazelnut allergens to varying extents. While 2D immunoblots indicated that roasting slightly reduced allergenicity, IgE ELISA with the pool of sera showed a slight significant (10%) increase in IgE binding in both gastric digesta. Cor a 9 isolated from the raw and roasted hazelnuts, characterized by far and near CD, remained stable after roasting, with preserved IgE reactivity. Its immunoreactivity contribution by inhibitory ELISA was noticeable in raw and roasted hazelnut digesta; its activity was slightly stronger in the roasted preparations. Roasting has a visible impact on proteins; however, it did not affect overall IgE reactivity. Gastric digestion slightly increases the overall IgE reactivity in raw and roasted hazelnuts, and may therefore impact the profiles of allergens and their fragments available to interact with the immune system in the small intestine. © 2022 by the authors.
PB  - MDPI
T2  - Foods
T1  - INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity
VL  - 11
SP  - 2914
DO  - 10.3390/foods11182914
ER  - 
@article{
author = "Prodić, Ivana and Smiljanić, Katarina and Nagl, Christoph and Ballmer-Weber, Barbara and Hoffmann-Sommergruber, Karin and Ćirković-Veličković, Tanja",
year = "2022",
abstract = "Most of the food allergens sensitized via the gastrointestinal tract resist thermal treatments and digestion, particularly digestion by pepsin. Roasted hazelnuts are more commonly consumed than raw ones. Since no studies have characterized gastric digestion protein fragments of raw and roasted hazelnuts nor their IgE binding properties, we compared these aspects of raw and roasted hazelnuts’ gastric digesta obtained by INFOGEST protocol. Their electrophoretically resolved profiles were probed with hazelnut allergic patients’ sera in 1D and 2D immunoblots. Electrophoretic profiles demonstrated pepsin digestion of all hazelnut allergens to varying extents. While 2D immunoblots indicated that roasting slightly reduced allergenicity, IgE ELISA with the pool of sera showed a slight significant (10%) increase in IgE binding in both gastric digesta. Cor a 9 isolated from the raw and roasted hazelnuts, characterized by far and near CD, remained stable after roasting, with preserved IgE reactivity. Its immunoreactivity contribution by inhibitory ELISA was noticeable in raw and roasted hazelnut digesta; its activity was slightly stronger in the roasted preparations. Roasting has a visible impact on proteins; however, it did not affect overall IgE reactivity. Gastric digestion slightly increases the overall IgE reactivity in raw and roasted hazelnuts, and may therefore impact the profiles of allergens and their fragments available to interact with the immune system in the small intestine. © 2022 by the authors.",
publisher = "MDPI",
journal = "Foods",
title = "INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity",
volume = "11",
pages = "2914",
doi = "10.3390/foods11182914"
}
Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods
MDPI., 11, 2914.
https://doi.org/10.3390/foods11182914
Prodić I, Smiljanić K, Nagl C, Ballmer-Weber B, Hoffmann-Sommergruber K, Ćirković-Veličković T. INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods. 2022;11:2914.
doi:10.3390/foods11182914 .
Prodić, Ivana, Smiljanić, Katarina, Nagl, Christoph, Ballmer-Weber, Barbara, Hoffmann-Sommergruber, Karin, Ćirković-Veličković, Tanja, "INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity" in Foods, 11 (2022):2914,
https://doi.org/10.3390/foods11182914 . .

Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914

Prodić, Ivana; Smiljanić, Katarina; Nagl, Christoph; Ballmer-Weber, Barbara; Hoffmann-Sommergruber, Karin; Ćirković-Veličković, Tanja

(MDPI, 2022)

TY  - DATA
AU  - Prodić, Ivana
AU  - Smiljanić, Katarina
AU  - Nagl, Christoph
AU  - Ballmer-Weber, Barbara
AU  - Hoffmann-Sommergruber, Karin
AU  - Ćirković-Veličković, Tanja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5657
AB  - Most of the food allergens sensitized via the gastrointestinal tract resist thermal treatments and digestion, particularly digestion by pepsin. Roasted hazelnuts are more commonly consumed than raw ones. Since no studies have characterized gastric digestion protein fragments of raw and roasted hazelnuts nor their IgE binding properties, we compared these aspects of raw and roasted hazelnuts’ gastric digesta obtained by INFOGEST protocol. Their electrophoretically resolved profiles were probed with hazelnut allergic patients’ sera in 1D and 2D immunoblots. Electrophoretic profiles demonstrated pepsin digestion of all hazelnut allergens to varying extents. While 2D immunoblots indicated that roasting slightly reduced allergenicity, IgE ELISA with the pool of sera showed a slight significant (10%) increase in IgE binding in both gastric digesta. Cor a 9 isolated from the raw and roasted hazelnuts, characterized by far and near CD, remained stable after roasting, with preserved IgE reactivity. Its immunoreactivity contribution by inhibitory ELISA was noticeable in raw and roasted hazelnut digesta; its activity was slightly stronger in the roasted preparations. Roasting has a visible impact on proteins; however, it did not affect overall IgE reactivity. Gastric digestion slightly increases the overall IgE reactivity in raw and roasted hazelnuts, and may therefore impact the profiles of allergens and their fragments available to interact with the immune system in the small intestine. © 2022 by the authors.
PB  - MDPI
T2  - Foods
T1  - Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914
VL  - 11
SP  - 2914
ER  - 
@misc{
author = "Prodić, Ivana and Smiljanić, Katarina and Nagl, Christoph and Ballmer-Weber, Barbara and Hoffmann-Sommergruber, Karin and Ćirković-Veličković, Tanja",
year = "2022",
abstract = "Most of the food allergens sensitized via the gastrointestinal tract resist thermal treatments and digestion, particularly digestion by pepsin. Roasted hazelnuts are more commonly consumed than raw ones. Since no studies have characterized gastric digestion protein fragments of raw and roasted hazelnuts nor their IgE binding properties, we compared these aspects of raw and roasted hazelnuts’ gastric digesta obtained by INFOGEST protocol. Their electrophoretically resolved profiles were probed with hazelnut allergic patients’ sera in 1D and 2D immunoblots. Electrophoretic profiles demonstrated pepsin digestion of all hazelnut allergens to varying extents. While 2D immunoblots indicated that roasting slightly reduced allergenicity, IgE ELISA with the pool of sera showed a slight significant (10%) increase in IgE binding in both gastric digesta. Cor a 9 isolated from the raw and roasted hazelnuts, characterized by far and near CD, remained stable after roasting, with preserved IgE reactivity. Its immunoreactivity contribution by inhibitory ELISA was noticeable in raw and roasted hazelnut digesta; its activity was slightly stronger in the roasted preparations. Roasting has a visible impact on proteins; however, it did not affect overall IgE reactivity. Gastric digestion slightly increases the overall IgE reactivity in raw and roasted hazelnuts, and may therefore impact the profiles of allergens and their fragments available to interact with the immune system in the small intestine. © 2022 by the authors.",
publisher = "MDPI",
journal = "Foods",
title = "Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914",
volume = "11",
pages = "2914"
}
Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914. in Foods
MDPI., 11, 2914.
Prodić I, Smiljanić K, Nagl C, Ballmer-Weber B, Hoffmann-Sommergruber K, Ćirković-Veličković T. Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914. in Foods. 2022;11:2914..
Prodić, Ivana, Smiljanić, Katarina, Nagl, Christoph, Ballmer-Weber, Barbara, Hoffmann-Sommergruber, Karin, Ćirković-Veličković, Tanja, "Supplementary material for:Prodić, I., Smiljanić, K., Nagl, C., Ballmer-Weber, B., Hoffmann-Sommergruber, K.,& Ćirković-Veličković, T.. (2022). INFOGEST Digestion Assay of Raw and Roasted Hazelnuts and Its Impact on Allergens and Their IgE Binding Activity. in Foods MDPI., 11, 2914. https://doi.org/10.3390/foods11182914" in Foods, 11 (2022):2914.

Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum

Ivanović, Stefan; Simić, Katarina; Lekić, Stefan; Jadranin, Milka; Vujisić, Ljubodrag; Gođevac, Dejan

(MDPI, 2022)

TY  - JOUR
AU  - Ivanović, Stefan
AU  - Simić, Katarina
AU  - Lekić, Stefan
AU  - Jadranin, Milka
AU  - Vujisić, Ljubodrag
AU  - Gođevac, Dejan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5655
AB  - Allium ursinum and poisonous adulterants Convallaria majalis and Arum maculatum were used as a model for detection of adulterants in edible plant. A. ursinum samples were spiked with C. majalis and A. maculatum to mimic adulteration. Metabolomic fingerprinting of all samples was performed using 1H NMR spectroscopy, and the resulting data sets were subjected to multivariate data analysis. As a result of this analysis, signals of adulterants were extracted from the data, and the structures of biomarkers of adulteration from partially purified samples were elucidated using 2D NMR and LC-MS techniques. Thus, isovitexin and vicenin II, azetidine-2-carboxylic acid, and trigonelline indicated adulteration of A. ursinum samples with C. majalis. Isovitexin was also recognized to be an indicator of adulteration of A. ursinum with A. maculatum. In conclusion, the case study of A. ursinum suggested that plant metabolomics approach could be utilized for identification of low molecular weight biomarkers of adulteration in edible plants. © 2022 by the authors.
PB  - MDPI
T2  - Metabolites
T1  - Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum
VL  - 12
SP  - 849
DO  - 10.3390/metabo12090849
ER  - 
@article{
author = "Ivanović, Stefan and Simić, Katarina and Lekić, Stefan and Jadranin, Milka and Vujisić, Ljubodrag and Gođevac, Dejan",
year = "2022",
abstract = "Allium ursinum and poisonous adulterants Convallaria majalis and Arum maculatum were used as a model for detection of adulterants in edible plant. A. ursinum samples were spiked with C. majalis and A. maculatum to mimic adulteration. Metabolomic fingerprinting of all samples was performed using 1H NMR spectroscopy, and the resulting data sets were subjected to multivariate data analysis. As a result of this analysis, signals of adulterants were extracted from the data, and the structures of biomarkers of adulteration from partially purified samples were elucidated using 2D NMR and LC-MS techniques. Thus, isovitexin and vicenin II, azetidine-2-carboxylic acid, and trigonelline indicated adulteration of A. ursinum samples with C. majalis. Isovitexin was also recognized to be an indicator of adulteration of A. ursinum with A. maculatum. In conclusion, the case study of A. ursinum suggested that plant metabolomics approach could be utilized for identification of low molecular weight biomarkers of adulteration in edible plants. © 2022 by the authors.",
publisher = "MDPI",
journal = "Metabolites",
title = "Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum",
volume = "12",
pages = "849",
doi = "10.3390/metabo12090849"
}
Ivanović, S., Simić, K., Lekić, S., Jadranin, M., Vujisić, L.,& Gođevac, D.. (2022). Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum. in Metabolites
MDPI., 12, 849.
https://doi.org/10.3390/metabo12090849
Ivanović S, Simić K, Lekić S, Jadranin M, Vujisić L, Gođevac D. Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum. in Metabolites. 2022;12:849.
doi:10.3390/metabo12090849 .
Ivanović, Stefan, Simić, Katarina, Lekić, Stefan, Jadranin, Milka, Vujisić, Ljubodrag, Gođevac, Dejan, "Plant Metabolomics as a Tool for Detecting Adulterants in Edible Plant: A Case Study of Allium ursinum" in Metabolites, 12 (2022):849,
https://doi.org/10.3390/metabo12090849 . .

Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions

Fotirić Akšić, Milica; Nešović, Milica; Ćirić, Ivanka; Tešić, Živoslav; Pezo, Lato; Tosti, Tomislav; Gašić, Uroš; Dojčinović, Biljana; Lončar, Biljana; Meland, Mekjell

(MDPI, 2022)

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5654
AB  - Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth
PB  - MDPI
T2  - Horticulturae
T1  - Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions
VL  - 8
SP  - 765
DO  - 10.3390/horticulturae8090765
ER  - 
@article{
author = "Fotirić Akšić, Milica and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth",
publisher = "MDPI",
journal = "Horticulturae",
title = "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions",
volume = "8",
pages = "765",
doi = "10.3390/horticulturae8090765"
}
Fotirić Akšić, M., Nešović, M., Ćirić, I., Tešić, Ž., Pezo, L., Tosti, T., Gašić, U., Dojčinović, B., Lončar, B.,& Meland, M.. (2022). Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae
MDPI., 8, 765.
https://doi.org/10.3390/horticulturae8090765
Fotirić Akšić M, Nešović M, Ćirić I, Tešić Ž, Pezo L, Tosti T, Gašić U, Dojčinović B, Lončar B, Meland M. Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae. 2022;8:765.
doi:10.3390/horticulturae8090765 .
Fotirić Akšić, Milica, Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav, Pezo, Lato, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Lončar, Biljana, Meland, Mekjell, "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions" in Horticulturae, 8 (2022):765,
https://doi.org/10.3390/horticulturae8090765 . .

Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705

Knežević, Sara; Ognjanović, Miloš; Stanković, Vesna; Zlatanova, Milena; Nešić, Andrijana; Gavrović-Jankulović, Marija; Stanković, Dalibor

(MDPI, 2022)

TY  - DATA
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5632
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705
VL  - 12
IS  - 9
SP  - 705
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5632
ER  - 
@misc{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705",
volume = "12",
number = "9",
pages = "705",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5632"
}
Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors
MDPI., 12(9), 705.
https://hdl.handle.net/21.15107/rcub_cherry_5632
Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić A, Gavrović-Jankulović M, Stanković D. Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors. 2022;12(9):705.
https://hdl.handle.net/21.15107/rcub_cherry_5632 .
Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana, Gavrović-Jankulović, Marija, Stanković, Dalibor, "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705" in Biosensors, 12, no. 9 (2022):705,
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells

Knežević, Sara; Ognjanović, Miloš; Stanković, Vesna; Zlatanova, Milena; Nešić, Andrijana; Gavrović-Jankulović, Marija; Stanković, Dalibor

(MDPI, 2022)

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5622
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells
VL  - 12
IS  - 9
SP  - 705
DO  - 10.3390/bios12090705
ER  - 
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells",
volume = "12",
number = "9",
pages = "705",
doi = "10.3390/bios12090705"
}
Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors
MDPI., 12(9), 705.
https://doi.org/10.3390/bios12090705
Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić A, Gavrović-Jankulović M, Stanković D. La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors. 2022;12(9):705.
doi:10.3390/bios12090705 .
Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana, Gavrović-Jankulović, Marija, Stanković, Dalibor, "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells" in Biosensors, 12, no. 9 (2022):705,
https://doi.org/10.3390/bios12090705 . .
1

When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times

Cvetković, Miljan; Kočić, Milana; Dabić Zagorac, Dragana; Ćirić, Ivanka; Natić, Maja; Hajder, Đurađ; Životić, Aleksandar; Fotirić Akšić, Milica

(MDPI, 2022)

TY  - JOUR
AU  - Cvetković, Miljan
AU  - Kočić, Milana
AU  - Dabić Zagorac, Dragana
AU  - Ćirić, Ivanka
AU  - Natić, Maja
AU  - Hajder, Đurađ
AU  - Životić, Aleksandar
AU  - Fotirić Akšić, Milica
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5651
AB  - Blueberries, which are recognized by their colored fruits and exquisite flavor and taste, are a great source of bioactive substances with potential functional properties. For the purpose of this study, the blueberry cultivars ‘Duke’, ‘Chandler’ and ‘Bluecrop’ were picked at four different times. The aim of the study was to compare the cultivars and determine the best time for picking fruits for table consumption and to produce berries that can be used as functional foods with elevated levels of bioactive compounds. According to principal component analysis (PCA), the most influential traits for distinguishing different times of harvest in the ‘Duke’ cultivar were sorbitol, glucose, sucrose, and turanose; for the cultivar ‘Chandler’, they were caffeic acid, aesculetin, and quercetin; for the ‘Bluecrop’, they were fructose, maltose, radical scavenging activity, and quercetin. Blueberry fruits aimed for table consumption were those harvested in the first two pickings of the cultivar ‘Duke’, in the first and third of the ‘Bluecrop’, and in the third picking time of the cultivar ‘Chandler’, due to the highest fruit size and very high level of sugar (mostly glucose and fructose). ‘Duke’ berries from the second and third harvest (high level of total phenolic content, radical scavenging activity, total anthocyanins, aesculin, quercetin, and isorhamnetin), ‘Chandler’ from the first and third (the highest p-hydroxybenzoic acid, aesculetin, caffeic acid, phloridzin, kaempferol, kaempferol 3-O-glucoside, quercetin 3-O-rhamnoside, rutin, and quercetin) and ‘Bluecrop’ from the third harvest (highest level of total phenolics, radical scavenging activity, quercetin, rutin, quercetin 3-O-glucoside, kaempferol, quercetin 3-O-rhamnoside, kaempferol 3-O-glucoside, and isorhamnetin) had the highest levels of health-promoting compounds.
PB  - MDPI
T2  - Metabolites
T1  - When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times
VL  - 12
SP  - 798
DO  - 10.3390/metabo12090798
ER  - 
@article{
author = "Cvetković, Miljan and Kočić, Milana and Dabić Zagorac, Dragana and Ćirić, Ivanka and Natić, Maja and Hajder, Đurađ and Životić, Aleksandar and Fotirić Akšić, Milica",
year = "2022",
abstract = "Blueberries, which are recognized by their colored fruits and exquisite flavor and taste, are a great source of bioactive substances with potential functional properties. For the purpose of this study, the blueberry cultivars ‘Duke’, ‘Chandler’ and ‘Bluecrop’ were picked at four different times. The aim of the study was to compare the cultivars and determine the best time for picking fruits for table consumption and to produce berries that can be used as functional foods with elevated levels of bioactive compounds. According to principal component analysis (PCA), the most influential traits for distinguishing different times of harvest in the ‘Duke’ cultivar were sorbitol, glucose, sucrose, and turanose; for the cultivar ‘Chandler’, they were caffeic acid, aesculetin, and quercetin; for the ‘Bluecrop’, they were fructose, maltose, radical scavenging activity, and quercetin. Blueberry fruits aimed for table consumption were those harvested in the first two pickings of the cultivar ‘Duke’, in the first and third of the ‘Bluecrop’, and in the third picking time of the cultivar ‘Chandler’, due to the highest fruit size and very high level of sugar (mostly glucose and fructose). ‘Duke’ berries from the second and third harvest (high level of total phenolic content, radical scavenging activity, total anthocyanins, aesculin, quercetin, and isorhamnetin), ‘Chandler’ from the first and third (the highest p-hydroxybenzoic acid, aesculetin, caffeic acid, phloridzin, kaempferol, kaempferol 3-O-glucoside, quercetin 3-O-rhamnoside, rutin, and quercetin) and ‘Bluecrop’ from the third harvest (highest level of total phenolics, radical scavenging activity, quercetin, rutin, quercetin 3-O-glucoside, kaempferol, quercetin 3-O-rhamnoside, kaempferol 3-O-glucoside, and isorhamnetin) had the highest levels of health-promoting compounds.",
publisher = "MDPI",
journal = "Metabolites",
title = "When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times",
volume = "12",
pages = "798",
doi = "10.3390/metabo12090798"
}
Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites
MDPI., 12, 798.
https://doi.org/10.3390/metabo12090798
Cvetković M, Kočić M, Dabić Zagorac D, Ćirić I, Natić M, Hajder Đ, Životić A, Fotirić Akšić M. When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites. 2022;12:798.
doi:10.3390/metabo12090798 .
Cvetković, Miljan, Kočić, Milana, Dabić Zagorac, Dragana, Ćirić, Ivanka, Natić, Maja, Hajder, Đurađ, Životić, Aleksandar, Fotirić Akšić, Milica, "When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times" in Metabolites, 12 (2022):798,
https://doi.org/10.3390/metabo12090798 . .

Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798

Cvetković, Miljan; Kočić, Milana; Dabić Zagorac, Dragana; Ćirić, Ivanka; Natić, Maja; Hajder, Đurađ; Životić, Aleksandar; Fotirić Akšić, Milica

(MDPI, 2022)

TY  - DATA
AU  - Cvetković, Miljan
AU  - Kočić, Milana
AU  - Dabić Zagorac, Dragana
AU  - Ćirić, Ivanka
AU  - Natić, Maja
AU  - Hajder, Đurađ
AU  - Životić, Aleksandar
AU  - Fotirić Akšić, Milica
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5651
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5652
AB  - Blueberries, which are recognized by their colored fruits and exquisite flavor and taste, are a great source of bioactive substances with potential functional properties. For the purpose of this study, the blueberry cultivars ‘Duke’, ‘Chandler’ and ‘Bluecrop’ were picked at four different times. The aim of the study was to compare the cultivars and determine the best time for picking fruits for table consumption and to produce berries that can be used as functional foods with elevated levels of bioactive compounds. According to principal component analysis (PCA), the most influential traits for distinguishing different times of harvest in the ‘Duke’ cultivar were sorbitol, glucose, sucrose, and turanose; for the cultivar ‘Chandler’, they were caffeic acid, aesculetin, and quercetin; for the ‘Bluecrop’, they were fructose, maltose, radical scavenging activity, and quercetin. Blueberry fruits aimed for table consumption were those harvested in the first two pickings of the cultivar ‘Duke’, in the first and third of the ‘Bluecrop’, and in the third picking time of the cultivar ‘Chandler’, due to the highest fruit size and very high level of sugar (mostly glucose and fructose). ‘Duke’ berries from the second and third harvest (high level of total phenolic content, radical scavenging activity, total anthocyanins, aesculin, quercetin, and isorhamnetin), ‘Chandler’ from the first and third (the highest p-hydroxybenzoic acid, aesculetin, caffeic acid, phloridzin, kaempferol, kaempferol 3-O-glucoside, quercetin 3-O-rhamnoside, rutin, and quercetin) and ‘Bluecrop’ from the third harvest (highest level of total phenolics, radical scavenging activity, quercetin, rutin, quercetin 3-O-glucoside, kaempferol, quercetin 3-O-rhamnoside, kaempferol 3-O-glucoside, and isorhamnetin) had the highest levels of health-promoting compounds.
PB  - MDPI
T2  - Metabolites
T1  - Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798
VL  - 12
SP  - 798
ER  - 
@misc{
author = "Cvetković, Miljan and Kočić, Milana and Dabić Zagorac, Dragana and Ćirić, Ivanka and Natić, Maja and Hajder, Đurađ and Životić, Aleksandar and Fotirić Akšić, Milica",
year = "2022",
abstract = "Blueberries, which are recognized by their colored fruits and exquisite flavor and taste, are a great source of bioactive substances with potential functional properties. For the purpose of this study, the blueberry cultivars ‘Duke’, ‘Chandler’ and ‘Bluecrop’ were picked at four different times. The aim of the study was to compare the cultivars and determine the best time for picking fruits for table consumption and to produce berries that can be used as functional foods with elevated levels of bioactive compounds. According to principal component analysis (PCA), the most influential traits for distinguishing different times of harvest in the ‘Duke’ cultivar were sorbitol, glucose, sucrose, and turanose; for the cultivar ‘Chandler’, they were caffeic acid, aesculetin, and quercetin; for the ‘Bluecrop’, they were fructose, maltose, radical scavenging activity, and quercetin. Blueberry fruits aimed for table consumption were those harvested in the first two pickings of the cultivar ‘Duke’, in the first and third of the ‘Bluecrop’, and in the third picking time of the cultivar ‘Chandler’, due to the highest fruit size and very high level of sugar (mostly glucose and fructose). ‘Duke’ berries from the second and third harvest (high level of total phenolic content, radical scavenging activity, total anthocyanins, aesculin, quercetin, and isorhamnetin), ‘Chandler’ from the first and third (the highest p-hydroxybenzoic acid, aesculetin, caffeic acid, phloridzin, kaempferol, kaempferol 3-O-glucoside, quercetin 3-O-rhamnoside, rutin, and quercetin) and ‘Bluecrop’ from the third harvest (highest level of total phenolics, radical scavenging activity, quercetin, rutin, quercetin 3-O-glucoside, kaempferol, quercetin 3-O-rhamnoside, kaempferol 3-O-glucoside, and isorhamnetin) had the highest levels of health-promoting compounds.",
publisher = "MDPI",
journal = "Metabolites",
title = "Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798",
volume = "12",
pages = "798"
}
Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798. in Metabolites
MDPI., 12, 798.
Cvetković M, Kočić M, Dabić Zagorac D, Ćirić I, Natić M, Hajder Đ, Životić A, Fotirić Akšić M. Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798. in Metabolites. 2022;12:798..
Cvetković, Miljan, Kočić, Milana, Dabić Zagorac, Dragana, Ćirić, Ivanka, Natić, Maja, Hajder, Đurađ, Životić, Aleksandar, Fotirić Akšić, Milica, "Supplementary material for:Cvetković, M., Kočić, M., Dabić Zagorac, D., Ćirić, I., Natić, M., Hajder, Đ., Životić, A.,& Fotirić Akšić, M.. (2022). When Is the Right Moment to Pick Blueberries? Variation in Agronomic and Chemical Properties of Blueberry (Vaccinium corymbosum) Cultivars at Different Harvest Times. in Metabolites MDPI., 12, 798. https://doi.org/10.3390/metabo12090798" in Metabolites, 12 (2022):798.

Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography

Šegan, Sandra; Jevtić, Ivana; Tosti, Tomislav; Penjišević, Jelena; Šukalović, Vladimir; Kostić-Rajačić, Slađana; Milojković-Opsenica, Dušanka

(Elsevier, 2022)

TY  - JOUR
AU  - Šegan, Sandra
AU  - Jevtić, Ivana
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić-Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5649
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-
layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the
piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-
reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant
of fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-
ophilicity, expressed as retention indices RM
0 , b, and C0, as well as PC1, was determined and correlated with in
silico values. Ionization constants were determined on the basis of the relationships between analyte’s retention
expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were
subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide
basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation
was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-
lecular weight. In general, results of the present research corroborate well with previously determined anti-
nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another
set of important parameters should be taken into account when designing new derivatives of C-3 substituted
fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC
can be considered as a valuable asset in the ligand-based drug design
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography
VL  - 1211
SP  - 123481
DO  - 10.1016/j.jchromb.2022.123481
ER  - 
@article{
author = "Šegan, Sandra and Jevtić, Ivana and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić-Rajačić, Slađana and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-
layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the
piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diaste-
reomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant
of fentanyl were also determined by the same method, as a reference. The physicochemical property, lip-
ophilicity, expressed as retention indices RM
0 , b, and C0, as well as PC1, was determined and correlated with in
silico values. Ionization constants were determined on the basis of the relationships between analyte’s retention
expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were
subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide
basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation
was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and mo-
lecular weight. In general, results of the present research corroborate well with previously determined anti-
nociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another
set of important parameters should be taken into account when designing new derivatives of C-3 substituted
fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC
can be considered as a valuable asset in the ligand-based drug design",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography",
volume = "1211",
pages = "123481",
doi = "10.1016/j.jchromb.2022.123481"
}
Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić-Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B
Elsevier., 1211, 123481.
https://doi.org/10.1016/j.jchromb.2022.123481
Šegan S, Jevtić I, Tosti T, Penjišević J, Šukalović V, Kostić-Rajačić S, Milojković-Opsenica D. Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B. 2022;1211:123481.
doi:10.1016/j.jchromb.2022.123481 .
Šegan, Sandra, Jevtić, Ivana, Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić-Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography" in Journal of Chromatography B, 1211 (2022):123481,
https://doi.org/10.1016/j.jchromb.2022.123481 . .

Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062

Ristivojević, Petar; Lekić, Nevena; Cvijetić, Ilija; Krstić, Đurđa; Andrić, Filip; Milojković-Opsenica, Dušanka; Morlock, Gertrud E.

(MDPI, 2022)

TY  - DATA
AU  - Ristivojević, Petar
AU  - Lekić, Nevena
AU  - Cvijetić, Ilija
AU  - Krstić, Đurđa
AU  - Andrić, Filip
AU  - Milojković-Opsenica, Dušanka
AU  - Morlock, Gertrud E.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5646
AB  - Strawberries are an important fruit in the European diet because of their unique taste and high content of essential nutrients and bioactive compounds. The anthocyanins are known to be colorful phenolics in strawberries. In 17 samples of six strawberry cultivars produced in Serbia, i.e., the common varieties Alba, Asia, and Clery as well as promising breeding materials (11.29.11, 11.34.6, and 11.39.3), the anthocyanin profile as well as antimicrobial and antioxidative activity profiles were determined. All investigated extracts showed antioxidative and antibacterial activities against Gram-negative Aliivibrio fischeri. The responses were quite similar in number and intensity. The HPTLC-DPPH• scavenging assay and HPTLC-Aliivibrio fischeri bioassay coupled with high-resolution mass spectrometry identified pelargonidin-3-O-glucoside (Pg-3-glc) as the main anthocyanin and prominent antioxidative and antimicrobial compound in strawberries. The density functional theory calculations at the M06-2X/6-31+G(d,p) level showed that Pg-3-glc quenches free radicals via sequential proton loss electron transfer mechanism in water and in pentyl ethanoate, where the 5-OH group is the most reactive site for proton and hydrogen atom transfer. The results were confirmed via spectrophotometry. The highest total phenolic content was found in Clery and 11.39.3, while statistically significant differences between the genotypes regarding the antioxidant activity were not confirmed. Although very similar in the anthocyanin, antioxidative, and antimicrobial profile patterns, the strawberry genotypes were successfully classified using principal component analysis.
PB  - MDPI
T2  - Molecules
T1  - Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062
VL  - 27
SP  - 6062
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5646
ER  - 
@misc{
author = "Ristivojević, Petar and Lekić, Nevena and Cvijetić, Ilija and Krstić, Đurđa and Andrić, Filip and Milojković-Opsenica, Dušanka and Morlock, Gertrud E.",
year = "2022",
abstract = "Strawberries are an important fruit in the European diet because of their unique taste and high content of essential nutrients and bioactive compounds. The anthocyanins are known to be colorful phenolics in strawberries. In 17 samples of six strawberry cultivars produced in Serbia, i.e., the common varieties Alba, Asia, and Clery as well as promising breeding materials (11.29.11, 11.34.6, and 11.39.3), the anthocyanin profile as well as antimicrobial and antioxidative activity profiles were determined. All investigated extracts showed antioxidative and antibacterial activities against Gram-negative Aliivibrio fischeri. The responses were quite similar in number and intensity. The HPTLC-DPPH• scavenging assay and HPTLC-Aliivibrio fischeri bioassay coupled with high-resolution mass spectrometry identified pelargonidin-3-O-glucoside (Pg-3-glc) as the main anthocyanin and prominent antioxidative and antimicrobial compound in strawberries. The density functional theory calculations at the M06-2X/6-31+G(d,p) level showed that Pg-3-glc quenches free radicals via sequential proton loss electron transfer mechanism in water and in pentyl ethanoate, where the 5-OH group is the most reactive site for proton and hydrogen atom transfer. The results were confirmed via spectrophotometry. The highest total phenolic content was found in Clery and 11.39.3, while statistically significant differences between the genotypes regarding the antioxidant activity were not confirmed. Although very similar in the anthocyanin, antioxidative, and antimicrobial profile patterns, the strawberry genotypes were successfully classified using principal component analysis.",
publisher = "MDPI",
journal = "Molecules",
title = "Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062",
volume = "27",
pages = "6062",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5646"
}
Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062. in Molecules
MDPI., 27, 6062.
https://hdl.handle.net/21.15107/rcub_cherry_5646
Ristivojević P, Lekić N, Cvijetić I, Krstić Đ, Andrić F, Milojković-Opsenica D, Morlock GE. Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062. in Molecules. 2022;27:6062.
https://hdl.handle.net/21.15107/rcub_cherry_5646 .
Ristivojević, Petar, Lekić, Nevena, Cvijetić, Ilija, Krstić, Đurđa, Andrić, Filip, Milojković-Opsenica, Dušanka, Morlock, Gertrud E., "Supplementary material for: Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules MDPI., 27, 6062. https://doi.org/10.3390/molecules27186062" in Molecules, 27 (2022):6062,
https://hdl.handle.net/21.15107/rcub_cherry_5646 .

Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics

Ristivojević, Petar; Lekić, Nevena; Cvijetić, Ilija; Krstić, Đurđa; Andrić, Filip; Milojković-Opsenica, Dušanka; Morlock, Gertrud E.

(MDPI, 2022)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Lekić, Nevena
AU  - Cvijetić, Ilija
AU  - Krstić, Đurđa
AU  - Andrić, Filip
AU  - Milojković-Opsenica, Dušanka
AU  - Morlock, Gertrud E.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5644
AB  - Strawberries are an important fruit in the European diet because of their unique taste and high content of essential nutrients and bioactive compounds. The anthocyanins are known to be colorful phenolics in strawberries. In 17 samples of six strawberry cultivars produced in Serbia, i.e., the common varieties Alba, Asia, and Clery as well as promising breeding materials (11.29.11, 11.34.6, and 11.39.3), the anthocyanin profile as well as antimicrobial and antioxidative activity profiles were determined. All investigated extracts showed antioxidative and antibacterial activities against Gram-negative Aliivibrio fischeri. The responses were quite similar in number and intensity. The HPTLC-DPPH• scavenging assay and HPTLC-Aliivibrio fischeri bioassay coupled with high-resolution mass spectrometry identified pelargonidin-3-O-glucoside (Pg-3-glc) as the main anthocyanin and prominent antioxidative and antimicrobial compound in strawberries. The density functional theory calculations at the M06-2X/6-31+G(d,p) level showed that Pg-3-glc quenches free radicals via sequential proton loss electron transfer mechanism in water and in pentyl ethanoate, where the 5-OH group is the most reactive site for proton and hydrogen atom transfer. The results were confirmed via spectrophotometry. The highest total phenolic content was found in Clery and 11.39.3, while statistically significant differences between the genotypes regarding the antioxidant activity were not confirmed. Although very similar in the anthocyanin, antioxidative, and antimicrobial profile patterns, the strawberry genotypes were successfully classified using principal component analysis.
PB  - MDPI
T2  - Molecules
T1  - Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics
VL  - 27
SP  - 6062
DO  - 10.3390/molecules27186062
ER  - 
@article{
author = "Ristivojević, Petar and Lekić, Nevena and Cvijetić, Ilija and Krstić, Đurđa and Andrić, Filip and Milojković-Opsenica, Dušanka and Morlock, Gertrud E.",
year = "2022",
abstract = "Strawberries are an important fruit in the European diet because of their unique taste and high content of essential nutrients and bioactive compounds. The anthocyanins are known to be colorful phenolics in strawberries. In 17 samples of six strawberry cultivars produced in Serbia, i.e., the common varieties Alba, Asia, and Clery as well as promising breeding materials (11.29.11, 11.34.6, and 11.39.3), the anthocyanin profile as well as antimicrobial and antioxidative activity profiles were determined. All investigated extracts showed antioxidative and antibacterial activities against Gram-negative Aliivibrio fischeri. The responses were quite similar in number and intensity. The HPTLC-DPPH• scavenging assay and HPTLC-Aliivibrio fischeri bioassay coupled with high-resolution mass spectrometry identified pelargonidin-3-O-glucoside (Pg-3-glc) as the main anthocyanin and prominent antioxidative and antimicrobial compound in strawberries. The density functional theory calculations at the M06-2X/6-31+G(d,p) level showed that Pg-3-glc quenches free radicals via sequential proton loss electron transfer mechanism in water and in pentyl ethanoate, where the 5-OH group is the most reactive site for proton and hydrogen atom transfer. The results were confirmed via spectrophotometry. The highest total phenolic content was found in Clery and 11.39.3, while statistically significant differences between the genotypes regarding the antioxidant activity were not confirmed. Although very similar in the anthocyanin, antioxidative, and antimicrobial profile patterns, the strawberry genotypes were successfully classified using principal component analysis.",
publisher = "MDPI",
journal = "Molecules",
title = "Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics",
volume = "27",
pages = "6062",
doi = "10.3390/molecules27186062"
}
Ristivojević, P., Lekić, N., Cvijetić, I., Krstić, Đ., Andrić, F., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules
MDPI., 27, 6062.
https://doi.org/10.3390/molecules27186062
Ristivojević P, Lekić N, Cvijetić I, Krstić Đ, Andrić F, Milojković-Opsenica D, Morlock GE. Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics. in Molecules. 2022;27:6062.
doi:10.3390/molecules27186062 .
Ristivojević, Petar, Lekić, Nevena, Cvijetić, Ilija, Krstić, Đurđa, Andrić, Filip, Milojković-Opsenica, Dušanka, Morlock, Gertrud E., "Effect-Directed Profiling of Strawberry Varieties and Breeding Materials via Planar Chromatography and Chemometrics" in Molecules, 27 (2022):6062,
https://doi.org/10.3390/molecules27186062 . .

The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study

Penjišević, Jelena Z.; Šukalović, Vladimir B.; Andrić, Deana; Suručić, Relja; Kostić-Rajačić, Slađana

(Springer, 2022)

TY  - JOUR
AU  - Penjišević, Jelena Z.
AU  - Šukalović, Vladimir B.
AU  - Andrić, Deana
AU  - Suručić, Relja
AU  - Kostić-Rajačić, Slađana
PY  - 2022
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35507251
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5181
AB  - Adrenergic receptors are among the most studied G protein-coupled receptors. Activation or blockade of these receptors is a major therapeutic approach for the treatment of numerous disorders such as cardiac hypertrophy, congestive heart failure, hypertension, angina pectoris, cardiac arrhythmias, depression, benign prostate hyperplasia, anaphylaxis, asthma, and hyperthyroidism. Among all nine cloned adrenoceptor subtypes and the subsequent development of animal models, a significant target for various neurological conditions treatment is alpha1-adrenergic receptors. 2-{[4-(2-Methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles, their 5 substituted derivatives, and structurally similar, arylpiperazine based alpha1-adrenergic receptors antagonists (trazodone, naftopidil, and urapidil) have been subjects of comparative analysis. Most of the novel compounds showed alpha1-adrenergic affinity in the range from 22 nM to 250 nM. The in silico docking and molecular dynamics simulations, binding data together with absorption, distribution, metabolism, and excretion (ADME) calculations identified the promising lead compounds. The results brought out the conclusions which allowed us to propose a rationale for the activity of these molecules and to highlight six compounds (2-5, 8, and 12) that exhibited an acceptable pharmacokinetic profile to the advanced investigation as the potential alpha1-adrenergic receptor antagonists.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study
VL  - n/a
DO  - 10.1007/s12010-022-03922-8
ER  - 
@article{
author = "Penjišević, Jelena Z. and Šukalović, Vladimir B. and Andrić, Deana and Suručić, Relja and Kostić-Rajačić, Slađana",
year = "2022",
abstract = "Adrenergic receptors are among the most studied G protein-coupled receptors. Activation or blockade of these receptors is a major therapeutic approach for the treatment of numerous disorders such as cardiac hypertrophy, congestive heart failure, hypertension, angina pectoris, cardiac arrhythmias, depression, benign prostate hyperplasia, anaphylaxis, asthma, and hyperthyroidism. Among all nine cloned adrenoceptor subtypes and the subsequent development of animal models, a significant target for various neurological conditions treatment is alpha1-adrenergic receptors. 2-{[4-(2-Methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles, their 5 substituted derivatives, and structurally similar, arylpiperazine based alpha1-adrenergic receptors antagonists (trazodone, naftopidil, and urapidil) have been subjects of comparative analysis. Most of the novel compounds showed alpha1-adrenergic affinity in the range from 22 nM to 250 nM. The in silico docking and molecular dynamics simulations, binding data together with absorption, distribution, metabolism, and excretion (ADME) calculations identified the promising lead compounds. The results brought out the conclusions which allowed us to propose a rationale for the activity of these molecules and to highlight six compounds (2-5, 8, and 12) that exhibited an acceptable pharmacokinetic profile to the advanced investigation as the potential alpha1-adrenergic receptor antagonists.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study",
volume = "n/a",
doi = "10.1007/s12010-022-03922-8"
}
Penjišević, J. Z., Šukalović, V. B., Andrić, D., Suručić, R.,& Kostić-Rajačić, S.. (2022). The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. in Applied Biochemistry and Biotechnology
Springer., n/a.
https://doi.org/10.1007/s12010-022-03922-8
Penjišević JZ, Šukalović VB, Andrić D, Suručić R, Kostić-Rajačić S. The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. in Applied Biochemistry and Biotechnology. 2022;n/a.
doi:10.1007/s12010-022-03922-8 .
Penjišević, Jelena Z., Šukalović, Vladimir B., Andrić, Deana, Suručić, Relja, Kostić-Rajačić, Slađana, "The Therapeutic Potential of 2-{[4-(2-methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study" in Applied Biochemistry and Biotechnology, n/a (2022),
https://doi.org/10.1007/s12010-022-03922-8 . .

Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8.

Penjišević, Jelena Z.; Šukalović, Vladimir B.; Andrić, Deana; Suručić, Relja; Kostić-Rajačić, Slađana V.

(Springer, 2022)

TY  - DATA
AU  - Penjišević, Jelena Z.
AU  - Šukalović, Vladimir B.
AU  - Andrić, Deana
AU  - Suručić, Relja
AU  - Kostić-Rajačić, Slađana V.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5182
AB  - Adrenergic receptors are among the most studied G protein-coupled receptors. Activation or blockade of these receptors is a major therapeutic approach for the treatment of numerous disorders such as cardiac hypertrophy, congestive heart failure, hypertension, angina pectoris, cardiac arrhythmias, depression, benign prostate hyperplasia, anaphylaxis, asthma, and hyperthyroidism. Among all nine cloned adrenoceptor subtypes and the subsequent development of animal models, a significant target for various neurological conditions treatment is alpha1-adrenergic receptors. 2-{[4-(2-Methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles, their 5 substituted derivatives, and structurally similar, arylpiperazine based alpha1-adrenergic receptors antagonists (trazodone, naftopidil, and urapidil) have been subjects of comparative analysis. Most of the novel compounds showed alpha1-adrenergic affinity in the range from 22 nM to 250 nM. The in silico docking and molecular dynamics simulations, binding data together with absorption, distribution, metabolism, and excretion (ADME) calculations identified the promising lead compounds. The results brought out the conclusions which allowed us to propose a rationale for the activity of these molecules and to highlight six compounds (2-5, 8, and 12) that exhibited an acceptable pharmacokinetic profile to the advanced investigation as the potential alpha1-adrenergic receptor antagonists.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5182
ER  - 
@misc{
author = "Penjišević, Jelena Z. and Šukalović, Vladimir B. and Andrić, Deana and Suručić, Relja and Kostić-Rajačić, Slađana V.",
year = "2022",
abstract = "Adrenergic receptors are among the most studied G protein-coupled receptors. Activation or blockade of these receptors is a major therapeutic approach for the treatment of numerous disorders such as cardiac hypertrophy, congestive heart failure, hypertension, angina pectoris, cardiac arrhythmias, depression, benign prostate hyperplasia, anaphylaxis, asthma, and hyperthyroidism. Among all nine cloned adrenoceptor subtypes and the subsequent development of animal models, a significant target for various neurological conditions treatment is alpha1-adrenergic receptors. 2-{[4-(2-Methoxyphenyl)piperazin-1-yl]alkyl}-1H-benzo[d]imidazoles, their 5 substituted derivatives, and structurally similar, arylpiperazine based alpha1-adrenergic receptors antagonists (trazodone, naftopidil, and urapidil) have been subjects of comparative analysis. Most of the novel compounds showed alpha1-adrenergic affinity in the range from 22 nM to 250 nM. The in silico docking and molecular dynamics simulations, binding data together with absorption, distribution, metabolism, and excretion (ADME) calculations identified the promising lead compounds. The results brought out the conclusions which allowed us to propose a rationale for the activity of these molecules and to highlight six compounds (2-5, 8, and 12) that exhibited an acceptable pharmacokinetic profile to the advanced investigation as the potential alpha1-adrenergic receptor antagonists.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5182"
}
Penjišević, J. Z., Šukalović, V. B., Andrić, D., Suručić, R.,& Kostić-Rajačić, S. V.. (2022). Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8.. in Applied Biochemistry and Biotechnology
Springer..
https://hdl.handle.net/21.15107/rcub_cherry_5182
Penjišević JZ, Šukalović VB, Andrić D, Suručić R, Kostić-Rajačić SV. Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8.. in Applied Biochemistry and Biotechnology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5182 .
Penjišević, Jelena Z., Šukalović, Vladimir B., Andrić, Deana, Suručić, Relja, Kostić-Rajačić, Slađana V., "Supplementary information for the article: Penjišević, J. Z.; Šukalović, V. B.; Andrić, D. B.; Suručić, R.; Kostić-Rajačić, S. V. The Therapeutic Potential of 2-{[4-(2-Methoxyphenyl)Piperazin-1-Yl]Alkyl}-1H-Benzo[d]Imidazoles as Ligands for Alpha1-Adrenergic Receptor - Comparative In Silico and In Vitro Study. Applied Biochemistry and Biotechnology 2022. https://doi.org/10.1007/s12010-022-03922-8." in Applied Biochemistry and Biotechnology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5182 .

Thin-layer chromatography in the authenticity testing of bee-products

Milojković-Opsenica, Dušanka; Trifković, Jelena; Ristivojević, Petar; Andrić, Filip

(Elsevier, 2022)

TY  - JOUR
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Ristivojević, Petar
AU  - Andrić, Filip
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4999
AB  - Quality control, nutritional value and the monitoring of hazardous residues in honey bee- products have become
major topics for both producers and consumers. Due to its potential role in human health, bee-products rich in
bioactive compounds are becoming increasingly popular. This review aims to provide an overview of thin-layer
chromatography methods used in quality control, authenticity testing and chemical profiling of bee-products in
order to help scientists engaged in the field of bee-products chemistry to utilize the advantages of this technique
in the detection and elimination of fraudulent practices in bee-product manufacturing. Recently, hyphenation of
thin-layer chromatography, image analysis and chemometrics support bee-products analysis by simultaneous determination of analytes with different detection principles, identification of individual
bioactive compounds as well as structure elucidation of compounds. Highlighted opportunities of thin-layer
chromatography could encourage further investigations that would lead to improvements in the detection and
elimination of marketing fraudulent practices.
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Thin-layer chromatography in the authenticity testing of bee-products
VL  - 1188
IS  - 123068
DO  - 10.1016/j.jchromb.2021.123068
ER  - 
@article{
author = "Milojković-Opsenica, Dušanka and Trifković, Jelena and Ristivojević, Petar and Andrić, Filip",
year = "2022",
abstract = "Quality control, nutritional value and the monitoring of hazardous residues in honey bee- products have become
major topics for both producers and consumers. Due to its potential role in human health, bee-products rich in
bioactive compounds are becoming increasingly popular. This review aims to provide an overview of thin-layer
chromatography methods used in quality control, authenticity testing and chemical profiling of bee-products in
order to help scientists engaged in the field of bee-products chemistry to utilize the advantages of this technique
in the detection and elimination of fraudulent practices in bee-product manufacturing. Recently, hyphenation of
thin-layer chromatography, image analysis and chemometrics support bee-products analysis by simultaneous determination of analytes with different detection principles, identification of individual
bioactive compounds as well as structure elucidation of compounds. Highlighted opportunities of thin-layer
chromatography could encourage further investigations that would lead to improvements in the detection and
elimination of marketing fraudulent practices.",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Thin-layer chromatography in the authenticity testing of bee-products",
volume = "1188",
number = "123068",
doi = "10.1016/j.jchromb.2021.123068"
}
Milojković-Opsenica, D., Trifković, J., Ristivojević, P.,& Andrić, F.. (2022). Thin-layer chromatography in the authenticity testing of bee-products. in Journal of Chromatography B
Elsevier., 1188(123068).
https://doi.org/10.1016/j.jchromb.2021.123068
Milojković-Opsenica D, Trifković J, Ristivojević P, Andrić F. Thin-layer chromatography in the authenticity testing of bee-products. in Journal of Chromatography B. 2022;1188(123068).
doi:10.1016/j.jchromb.2021.123068 .
Milojković-Opsenica, Dušanka, Trifković, Jelena, Ristivojević, Petar, Andrić, Filip, "Thin-layer chromatography in the authenticity testing of bee-products" in Journal of Chromatography B, 1188, no. 123068 (2022),
https://doi.org/10.1016/j.jchromb.2021.123068 . .
1
4
3
3

Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale

Ristivojević, Petar; Andrić, Filip; Vasić, Vesna; Milojković-Opsenica, Dušanka; Morlock, Gertrud E.

(Elsevier, 2022)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Andrić, Filip
AU  - Vasić, Vesna
AU  - Milojković-Opsenica, Dušanka
AU  - Morlock, Gertrud E.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5000
AB  - The European Commission requires that fruit products distributed on the market meet standards of high quality and authenticity. For the quality assessment of apricot products susceptible to food fraud, an environmentally friendly, simple and cost-effective analytical profiling was developed by high-performance thin-layer chromatography multi-imaging (HPTLC-FLD/Vis). The new phytochemical profiling was applied for analysis of authentic samples (7 apricot and 5 pumpkin samples) and simulated adulterated products (11 mixture samples prepared by addition of 2.5–53% pooled pumpkin to pooled apricot). Based on the analytical eco-scale assessment, the HPTLC-FLD/Vis method was proven as an excellent green analytical method with low energy and solvent consumption. Chemometric data analysis confirmed the difference between apricot and apricot-pumpkin mixtures based on the phytochemical profile. Chemical markers responsible for their differentiation were identified. The results indicated that frauds by adding pumpkin to apricot products can be detected at added contents as low as 2.5%.
PB  - Elsevier
T2  - Food Chemistry
T1  - Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale
VL  - 374
IS  - 131714
DO  - 10.1016/j.foodchem.2021.131714
ER  - 
@article{
author = "Ristivojević, Petar and Andrić, Filip and Vasić, Vesna and Milojković-Opsenica, Dušanka and Morlock, Gertrud E.",
year = "2022",
abstract = "The European Commission requires that fruit products distributed on the market meet standards of high quality and authenticity. For the quality assessment of apricot products susceptible to food fraud, an environmentally friendly, simple and cost-effective analytical profiling was developed by high-performance thin-layer chromatography multi-imaging (HPTLC-FLD/Vis). The new phytochemical profiling was applied for analysis of authentic samples (7 apricot and 5 pumpkin samples) and simulated adulterated products (11 mixture samples prepared by addition of 2.5–53% pooled pumpkin to pooled apricot). Based on the analytical eco-scale assessment, the HPTLC-FLD/Vis method was proven as an excellent green analytical method with low energy and solvent consumption. Chemometric data analysis confirmed the difference between apricot and apricot-pumpkin mixtures based on the phytochemical profile. Chemical markers responsible for their differentiation were identified. The results indicated that frauds by adding pumpkin to apricot products can be detected at added contents as low as 2.5%.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale",
volume = "374",
number = "131714",
doi = "10.1016/j.foodchem.2021.131714"
}
Ristivojević, P., Andrić, F., Vasić, V., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale. in Food Chemistry
Elsevier., 374(131714).
https://doi.org/10.1016/j.foodchem.2021.131714
Ristivojević P, Andrić F, Vasić V, Milojković-Opsenica D, Morlock GE. Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale. in Food Chemistry. 2022;374(131714).
doi:10.1016/j.foodchem.2021.131714 .
Ristivojević, Petar, Andrić, Filip, Vasić, Vesna, Milojković-Opsenica, Dušanka, Morlock, Gertrud E., "Fast detection of apricot product frauds by added pumpkin via planar chromatography and chemometrics: Greenness assessment by analytical eco-scale" in Food Chemistry, 374, no. 131714 (2022),
https://doi.org/10.1016/j.foodchem.2021.131714 . .
1
1
1

Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714.

Ristivojević, Petar; Andrić, Filip; Vasić, Vesna; Milojković-Opsenica, Dušanka; Morlock, Gertrud E.

(Elsevier, 2022)

TY  - DATA
AU  - Ristivojević, Petar
AU  - Andrić, Filip
AU  - Vasić, Vesna
AU  - Milojković-Opsenica, Dušanka
AU  - Morlock, Gertrud E.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5001
PB  - Elsevier
T2  - Food Chemistry
T1  - Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5001
ER  - 
@misc{
author = "Ristivojević, Petar and Andrić, Filip and Vasić, Vesna and Milojković-Opsenica, Dušanka and Morlock, Gertrud E.",
year = "2022",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5001"
}
Ristivojević, P., Andrić, F., Vasić, V., Milojković-Opsenica, D.,& Morlock, G. E.. (2022). Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714.. in Food Chemistry
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_5001
Ristivojević P, Andrić F, Vasić V, Milojković-Opsenica D, Morlock GE. Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714.. in Food Chemistry. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5001 .
Ristivojević, Petar, Andrić, Filip, Vasić, Vesna, Milojković-Opsenica, Dušanka, Morlock, Gertrud E., "Supplementary data for the article: Ristivojević, P.; Andrić, F.; Vasić, V.; Milojković Opsenica, D.; Morlock, G. Fast Detection of Apricot Product Frauds by Added Pumpkin via Planar Chromatography and Chemometrics: Greenness Assessment by Analytical Eco-Scale. Food Chemistry 2022, 374 (131714). https://doi.org/10.1016/j.foodchem.2021.131714." in Food Chemistry (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5001 .

Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits

Krstić, Đurđa D.; Milinčić, Danijel; Kostić, Aleksandar Ž.; Fotirić-Akšić, Milica M.; Stanojević, Slađana P.; Milojković-Opsenica, Dušanka; Pešić, Mirjana; Trifković, Jelena

(Elsevier, 2022)

TY  - JOUR
AU  - Krstić, Đurđa D.
AU  - Milinčić, Danijel
AU  - Kostić, Aleksandar Ž.
AU  - Fotirić-Akšić, Milica M.
AU  - Stanojević, Slađana P.
AU  - Milojković-Opsenica, Dušanka
AU  - Pešić, Mirjana
AU  - Trifković, Jelena
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5196
AB  - Product authentication is one of the most important food quality assurances. Considering the importance of
consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in
bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein
fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars
(strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and
gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The
most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4;
30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds
proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of
berry-based products adulteration
PB  - Elsevier
T2  - Food Chemistry
T1  - Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits
VL  - 383
SP  - 132583
DO  - 10.1016/j.foodchem.2022.132583
ER  - 
@article{
author = "Krstić, Đurđa D. and Milinčić, Danijel and Kostić, Aleksandar Ž. and Fotirić-Akšić, Milica M. and Stanojević, Slađana P. and Milojković-Opsenica, Dušanka and Pešić, Mirjana and Trifković, Jelena",
year = "2022",
abstract = "Product authentication is one of the most important food quality assurances. Considering the importance of
consumption of berry fruits with proven health-beneficial properties, high sensory values and rich composition in
bioactive substances, the aim of this study was to evaluate a straightforward and simple procedure for the protein
fingerprinting of berry seeds. For this purpose, protein profiles of 45 samples of genuine berry fruit cultivars
(strawberry, raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry, and
gojiberry) were analyzed by SDS-PAGE electrophoresis in combination with advanced chemometric tools. The
most important parameters for discrimination among berry seeds were polypeptides at 12.8; 15.1; 25.0; 26.4;
30.0; 41.8; 44.4; 46.0; 48.5; 52.3 and 56.4 kDa. Biomarkers obtained from the protein profile of berry seeds
proved to be a powerful tool in the authentication of their botanical origin, as well as for potential detection of
berry-based products adulteration",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits",
volume = "383",
pages = "132583",
doi = "10.1016/j.foodchem.2022.132583"
}
Krstić, Đ. D., Milinčić, D., Kostić, A. Ž., Fotirić-Akšić, M. M., Stanojević, S. P., Milojković-Opsenica, D., Pešić, M.,& Trifković, J.. (2022). Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry
Elsevier., 383, 132583.
https://doi.org/10.1016/j.foodchem.2022.132583
Krstić ĐD, Milinčić D, Kostić AŽ, Fotirić-Akšić MM, Stanojević SP, Milojković-Opsenica D, Pešić M, Trifković J. Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits. in Food Chemistry. 2022;383:132583.
doi:10.1016/j.foodchem.2022.132583 .
Krstić, Đurđa D., Milinčić, Danijel, Kostić, Aleksandar Ž., Fotirić-Akšić, Milica M., Stanojević, Slađana P., Milojković-Opsenica, Dušanka, Pešić, Mirjana, Trifković, Jelena, "Comprehensive electrophoretic profiling of proteins as a powerful tool for authenticity assessment of seeds of cultivated berry fruits" in Food Chemistry, 383 (2022):132583,
https://doi.org/10.1016/j.foodchem.2022.132583 . .
1

Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus

Ljoljić Bilić, Vanja; Gašić, Uroš M.; Milojković-Opsenica, Dušanka; Rimac, Hrvoje; Vuković Rodriguez, Jadranka; Vlainić, Josipa; Brlek‐Gorski, Diana; Kosalec, Ivan

(MDPI, 2022)

TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Rimac, Hrvoje
AU  - Vuković Rodriguez, Jadranka
AU  - Vlainić, Josipa
AU  - Brlek‐Gorski, Diana
AU  - Kosalec, Ivan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5197
AB  - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation
PB  - MDPI
T2  - Аntibiotics
T1  - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
VL  - 11
SP  - 492
DO  - 10.3390/antibiotics11040492
ER  - 
@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek‐Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation",
publisher = "MDPI",
journal = "Аntibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
volume = "11",
pages = "492",
doi = "10.3390/antibiotics11040492"
}
Ljoljić Bilić, V., Gašić, U. M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek‐Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics
MDPI., 11, 492.
https://doi.org/10.3390/antibiotics11040492
Ljoljić Bilić V, Gašić UM, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek‐Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics. 2022;11:492.
doi:10.3390/antibiotics11040492 .
Ljoljić Bilić, Vanja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek‐Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Аntibiotics, 11 (2022):492,
https://doi.org/10.3390/antibiotics11040492 . .
1

Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro

Jurič, Andreja; Brčić Karačonji, Irena; Žunec, Suzana; Katić, Anja; Gašić, Uroš M.; Milojković-Opsenica, Dušanka; Kopjar, Nevenka

(Taylor and Francis, 2022)

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Žunec, Suzana
AU  - Katić, Anja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Kopjar, Nevenka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5199
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
SP  - 1
EP  - 10
DO  - 10.1080/00218839.2022.2047421
ER  - 
@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Žunec, Suzana and Katić, Anja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Kopjar, Nevenka",
year = "2022",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.",
publisher = "Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro",
pages = "1-10",
doi = "10.1080/00218839.2022.2047421"
}
Jurič, A., Brčić Karačonji, I., Žunec, S., Katić, A., Gašić, U. M., Milojković-Opsenica, D.,& Kopjar, N.. (2022). Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research
Taylor and Francis., 1-10.
https://doi.org/10.1080/00218839.2022.2047421
Jurič A, Brčić Karačonji I, Žunec S, Katić A, Gašić UM, Milojković-Opsenica D, Kopjar N. Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research. 2022;:1-10.
doi:10.1080/00218839.2022.2047421 .
Jurič, Andreja, Brčić Karačonji, Irena, Žunec, Suzana, Katić, Anja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Kopjar, Nevenka, "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro" in Journal of Apicultural Research (2022):1-10,
https://doi.org/10.1080/00218839.2022.2047421 . .
1
1

Honey with added value – enriched with rutin and quercetin from Sophora flower

Đorđević, Sofija; Nedić, Nebojša; Pavlović, Aleksandra; Milojković-Opsenica, Dušanka; Tešić, Živoslav; Gašić, Uroš M.

(Elsevier, 2022)

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš M.
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S2210803322000495
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5410
AB  - IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
PB  - Elsevier
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
SP  - 100580
DO  - 10.1016/j.hermed.2022.100580
ER  - 
@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš M.",
year = "2022",
abstract = "IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
publisher = "Elsevier",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
pages = "100580",
doi = "10.1016/j.hermed.2022.100580"
}
Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U. M.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine
Elsevier., 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580
Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić UM. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .
Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš M., "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time

Koprivica, Marija; Milojković-Opsenica, Dušanka; Fotirić Akšić, Milica; Dramićanin, Aleksandra M.; Lazarević, Kristina

(2022)

TY  - JOUR
AU  - Koprivica, Marija
AU  - Milojković-Opsenica, Dušanka
AU  - Fotirić Akšić, Milica
AU  - Dramićanin, Aleksandra M.
AU  - Lazarević, Kristina
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5498
AB  - The peach stones and kernels are easily available biowaste which could be useful for the extraction of nutritionally important compounds such as fatty acids. Except in industry, characterization of stones and kernels could be useful in pomology to describe different cultivars, and for selecting new parents in a breeding program. A total of 25 samples of stones and kernels from various peach cultivars that differed in origin and ripening time, but growing in the same climatic conditions, were characterized by fatty acids composition and physical properties. This work confirmed that unsaturated fatty acids (oleic and linoleic fatty acids) were the most represented in peach kernel oil and their content depended of peach genotype. Additionally, the fatty acids in combination with length, weight, and moisture of peach kernels could be used as a parameter of authenticity assessment. This research may contribute for the peach cultivar discrimination and recommendation of cultivars/genotypes with high kernel quality which could be used for the extractions of oil rich in unsaturated fatty acids and further use in food, pharmaceutical and cosmetic industry. Besides, selected cultivars could be used in breeding programs, for creating new genotypes for oil production.
T2  - European Food Research and Technology
T1  - Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time
VL  - 248
SP  - 2471
EP  - 2482
DO  - 10.1007/s00217-022-04062-3
ER  - 
@article{
author = "Koprivica, Marija and Milojković-Opsenica, Dušanka and Fotirić Akšić, Milica and Dramićanin, Aleksandra M. and Lazarević, Kristina",
year = "2022",
abstract = "The peach stones and kernels are easily available biowaste which could be useful for the extraction of nutritionally important compounds such as fatty acids. Except in industry, characterization of stones and kernels could be useful in pomology to describe different cultivars, and for selecting new parents in a breeding program. A total of 25 samples of stones and kernels from various peach cultivars that differed in origin and ripening time, but growing in the same climatic conditions, were characterized by fatty acids composition and physical properties. This work confirmed that unsaturated fatty acids (oleic and linoleic fatty acids) were the most represented in peach kernel oil and their content depended of peach genotype. Additionally, the fatty acids in combination with length, weight, and moisture of peach kernels could be used as a parameter of authenticity assessment. This research may contribute for the peach cultivar discrimination and recommendation of cultivars/genotypes with high kernel quality which could be used for the extractions of oil rich in unsaturated fatty acids and further use in food, pharmaceutical and cosmetic industry. Besides, selected cultivars could be used in breeding programs, for creating new genotypes for oil production.",
journal = "European Food Research and Technology",
title = "Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time",
volume = "248",
pages = "2471-2482",
doi = "10.1007/s00217-022-04062-3"
}
Koprivica, M., Milojković-Opsenica, D., Fotirić Akšić, M., Dramićanin, A. M.,& Lazarević, K.. (2022). Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time. in European Food Research and Technology, 248, 2471-2482.
https://doi.org/10.1007/s00217-022-04062-3
Koprivica M, Milojković-Opsenica D, Fotirić Akšić M, Dramićanin AM, Lazarević K. Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time. in European Food Research and Technology. 2022;248:2471-2482.
doi:10.1007/s00217-022-04062-3 .
Koprivica, Marija, Milojković-Opsenica, Dušanka, Fotirić Akšić, Milica, Dramićanin, Aleksandra M., Lazarević, Kristina, "Fatty acids composition and physical properties of stones and kernels from different peach cultivars as biomarker of origin and ripening time" in European Food Research and Technology, 248 (2022):2471-2482,
https://doi.org/10.1007/s00217-022-04062-3 . .

Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage

Vukadinović, Jelena; Srdić, Jelena; Tosti, Tomislav; Dragičević, Vesna; Kravić, Natalija; Drinić, Snežana Mladenović; Milojković-Opsenica, Dušanka

(Elsevier, 2022)

TY  - JOUR
AU  - Vukadinović, Jelena
AU  - Srdić, Jelena
AU  - Tosti, Tomislav
AU  - Dragičević, Vesna
AU  - Kravić, Natalija
AU  - Drinić, Snežana Mladenović
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5513
AB  - Sweet maize has worldwide importance due to its high nutritional value and health benefits. In order to provide valuable health-related information to consumers, the phytochemicals profile of raw sweet maize kernels and those exposed to different treatments (cooking, frozen storage before cooking, sucrose addition during cooking, and blanching) was examined herein. Thus, carotenoids, tocopherols and free phenolic acids content was determined by liquid chromatography, while the content of water soluble sugars was analyzed by anion exchange chromatography. In response to immediate cooking, as well as, to blanching prior to shorter frozen storage and cooking afterwards, all hybrids exhibited a significant decrease in carotenoids, and a significant increase in concentration of tocopherols, fructose, glucose, protocatechuic, ferulic and p-coumaric acids, in comparison to the fresh samples. However, treatments with blanching prior to longer frozen storage and cooking afterwards, contributed only to increased tocopherols content. Distinct grouping of processed samples in comparison to their corresponding control samples, revealed the existence of unique linkage between changes in phytochemicals content upon treatment in a hybrid-dependent manner. Combined blanching prior to shorter frozen storage with sucrose addition during cooking is highly recommended as a potent tool for phytochemicals enhancement.
PB  - Elsevier
T2  - Journal of Food Composition and Analysis
T1  - Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage
VL  - 114
SP  - 104637
DO  - 10.1016/j.jfca.2022.104637
ER  - 
@article{
author = "Vukadinović, Jelena and Srdić, Jelena and Tosti, Tomislav and Dragičević, Vesna and Kravić, Natalija and Drinić, Snežana Mladenović and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Sweet maize has worldwide importance due to its high nutritional value and health benefits. In order to provide valuable health-related information to consumers, the phytochemicals profile of raw sweet maize kernels and those exposed to different treatments (cooking, frozen storage before cooking, sucrose addition during cooking, and blanching) was examined herein. Thus, carotenoids, tocopherols and free phenolic acids content was determined by liquid chromatography, while the content of water soluble sugars was analyzed by anion exchange chromatography. In response to immediate cooking, as well as, to blanching prior to shorter frozen storage and cooking afterwards, all hybrids exhibited a significant decrease in carotenoids, and a significant increase in concentration of tocopherols, fructose, glucose, protocatechuic, ferulic and p-coumaric acids, in comparison to the fresh samples. However, treatments with blanching prior to longer frozen storage and cooking afterwards, contributed only to increased tocopherols content. Distinct grouping of processed samples in comparison to their corresponding control samples, revealed the existence of unique linkage between changes in phytochemicals content upon treatment in a hybrid-dependent manner. Combined blanching prior to shorter frozen storage with sucrose addition during cooking is highly recommended as a potent tool for phytochemicals enhancement.",
publisher = "Elsevier",
journal = "Journal of Food Composition and Analysis",
title = "Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage",
volume = "114",
pages = "104637",
doi = "10.1016/j.jfca.2022.104637"
}
Vukadinović, J., Srdić, J., Tosti, T., Dragičević, V., Kravić, N., Drinić, S. M.,& Milojković-Opsenica, D.. (2022). Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage. in Journal of Food Composition and Analysis
Elsevier., 114, 104637.
https://doi.org/10.1016/j.jfca.2022.104637
Vukadinović J, Srdić J, Tosti T, Dragičević V, Kravić N, Drinić SM, Milojković-Opsenica D. Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage. in Journal of Food Composition and Analysis. 2022;114:104637.
doi:10.1016/j.jfca.2022.104637 .
Vukadinović, Jelena, Srdić, Jelena, Tosti, Tomislav, Dragičević, Vesna, Kravić, Natalija, Drinić, Snežana Mladenović, Milojković-Opsenica, Dušanka, "Alteration in phytochemicals from sweet maize in response to domestic cooking and frozen storage" in Journal of Food Composition and Analysis, 114 (2022):104637,
https://doi.org/10.1016/j.jfca.2022.104637 . .
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