TY  - JOUR
AU  - Goff, Miriam
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin E.
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/607
AB  - A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1).
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - FEMS Microbiology Letters / Federation of European Microbiological Societies
T1  - Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation
VL  - 292
IS  - 2
SP  - 297
EP  - 305
DO  - 10.1111/j.1574-6968.2009.01504.x
ER  - 

@article{
author = "Goff, Miriam and Nikodinović-Runić, Jasmina and O'Connor, Kevin E.",
year = "2009",
abstract = "A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1).",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "FEMS Microbiology Letters / Federation of European Microbiological Societies",
title = "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation",
volume = "292",
number = "2",
pages = "297-305",
doi = "10.1111/j.1574-6968.2009.01504.x"
}

Goff, M., Nikodinović-Runić, J.,& O'Connor, K. E.. (2009). Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters / Federation of European Microbiological Societies
Wiley-Blackwell Publishing, Inc, Malden., 292(2), 297-305.
https://doi.org/10.1111/j.1574-6968.2009.01504.x

Goff M, Nikodinović-Runić J, O'Connor KE. Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters / Federation of European Microbiological Societies. 2009;292(2):297-305.
doi:10.1111/j.1574-6968.2009.01504.x .

Goff, Miriam, Nikodinović-Runić, Jasmina, O'Connor, Kevin E., "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation" in FEMS Microbiology Letters / Federation of European Microbiological Societies, 292, no. 2 (2009):297-305,
https://doi.org/10.1111/j.1574-6968.2009.01504.x . .