TY  - CONF
AU  - Radović, Nebojša R.
AU  - Stojanović, Ksenija A.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6053
AB  - Introduction: Metabolites from Curcuma longa show pH-dependent color-changing properties. During
this study, test strips were developed using Curcuma longa metabolites, which enable the rapid estima-
tion of acidity/alkalinity in natural and artificial samples.
Methods: Commercially available Curcuma longa powder (5.00 g) was mixed with ethanol (45 mL) and
subjected to 30 minutes of ultrasonic extraction. After 60 minutes of settling, the resulting suspension
was filtered and supplemented with ethanol to reach a final volume of 50 mL. Circular pieces of filter
paper were immersed in 15 mL of the colored filtrate in Petri dishes for 10 minutes. The impregnated
pieces of filter paper were then dried at 65°C for 10 minutes and cut into desired rectangular shapes.
Results: Analysis of the prepared test strips’ behavior was conducted across a pH range from 0 to 14, en-
compassing various solutions (HCl, NaOH, and buffered solutions) whose pH values were measured by
a pH meter. The test strips exhibited a yellow-orange color at pH values below 8.5, while a brown color
was observed at pH values of 8.5 and above.
Conclusion: The experimental data obtained in this investigation demonstrate significant agreement
with the literature value for the first pKa of curcumin (pKa1=8.4), a compound displaying the distinctive
orange color found in dry Curcuma longa powder, and possessing pH-dependent color-changing char-
acteristics. Therefore, test strips prepared from an ethanolic extract of Curcuma longa powder constitute
a promising tool for the routine assessment of acidity/alkalinity across various samples in molecular bi-
ology, (bio)chemistry, pharmacy, medicine, and related fields.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue
T1  - Utilizing metabolites from curcuma longa for the development of ph-responsive test strips
SP  - 164
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6053
ER  - 

@conference{
author = "Radović, Nebojša R. and Stojanović, Ksenija A.",
year = "2023",
abstract = "Introduction: Metabolites from Curcuma longa show pH-dependent color-changing properties. During
this study, test strips were developed using Curcuma longa metabolites, which enable the rapid estima-
tion of acidity/alkalinity in natural and artificial samples.
Methods: Commercially available Curcuma longa powder (5.00 g) was mixed with ethanol (45 mL) and
subjected to 30 minutes of ultrasonic extraction. After 60 minutes of settling, the resulting suspension
was filtered and supplemented with ethanol to reach a final volume of 50 mL. Circular pieces of filter
paper were immersed in 15 mL of the colored filtrate in Petri dishes for 10 minutes. The impregnated
pieces of filter paper were then dried at 65°C for 10 minutes and cut into desired rectangular shapes.
Results: Analysis of the prepared test strips’ behavior was conducted across a pH range from 0 to 14, en-
compassing various solutions (HCl, NaOH, and buffered solutions) whose pH values were measured by
a pH meter. The test strips exhibited a yellow-orange color at pH values below 8.5, while a brown color
was observed at pH values of 8.5 and above.
Conclusion: The experimental data obtained in this investigation demonstrate significant agreement
with the literature value for the first pKa of curcumin (pKa1=8.4), a compound displaying the distinctive
orange color found in dry Curcuma longa powder, and possessing pH-dependent color-changing char-
acteristics. Therefore, test strips prepared from an ethanolic extract of Curcuma longa powder constitute
a promising tool for the routine assessment of acidity/alkalinity across various samples in molecular bi-
ology, (bio)chemistry, pharmacy, medicine, and related fields.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue",
title = "Utilizing metabolites from curcuma longa for the development of ph-responsive test strips",
pages = "164-164",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6053"
}

Radović, N. R.,& Stojanović, K. A.. (2023). Utilizing metabolites from curcuma longa for the development of ph-responsive test strips. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 164-164.
https://hdl.handle.net/21.15107/rcub_cherry_6053

Radović NR, Stojanović KA. Utilizing metabolites from curcuma longa for the development of ph-responsive test strips. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue. 2023;:164-164.
https://hdl.handle.net/21.15107/rcub_cherry_6053 .

Radović, Nebojša R., Stojanović, Ksenija A., "Utilizing metabolites from curcuma longa for the development of ph-responsive test strips" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue (2023):164-164,
https://hdl.handle.net/21.15107/rcub_cherry_6053 .