Development of new and improvement of existing electrochemical, spectroscopic and flow injection (FIA) methods on environmental quality monitoring

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Development of new and improvement of existing electrochemical, spectroscopic and flow injection (FIA) methods on environmental quality monitoring (en)
Развој нових и побољшање постојећих електрохемијских, спектроскопских и проточних (FIA) метода за праћење квалитета животне средине (sr)
Razvoj novih i poboljšanje postojećih elektrohemijskih, spektroskopskih i protočnih (FIA) metoda za praćenje kvaliteta životne sredine (sr_RS)
Authors

Publications

Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors

Vrhovac, Lidija; Šelemetjev, Sonja A.; Vatić, Saša; Mitrović, Aleksandar; Milošević, Jelica; Lolić, Aleksandar; Beletić, Anđelo D.; Polović, Natalija

(Elsevier, 2021)

TY  - JOUR
AU  - Vrhovac, Lidija
AU  - Šelemetjev, Sonja A.
AU  - Vatić, Saša
AU  - Mitrović, Aleksandar
AU  - Milošević, Jelica
AU  - Lolić, Aleksandar
AU  - Beletić, Anđelo D.
AU  - Polović, Natalija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4295
AB  - Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.
PB  - Elsevier
T2  - Talanta
T1  - Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors
VL  - 223
SP  - 121588
DO  - 10.1016/j.talanta.2020.121588
ER  - 
@article{
author = "Vrhovac, Lidija and Šelemetjev, Sonja A. and Vatić, Saša and Mitrović, Aleksandar and Milošević, Jelica and Lolić, Aleksandar and Beletić, Anđelo D. and Polović, Natalija",
year = "2021",
abstract = "Measurement of antithyroglobulin antibodies (TgAb) is an inevitable laboratory tool in the management of thyroid gland diseases. Currently available immunoassays still have limitations underlying the necessity of the introduction of fast, sensitive, and label-free technologies. Our aim was to develop a method for TgAb measurement in human serum based on the quartz crystal microbalance (QCM) technology. We immobilized thyroglobulin on the surface of Attana LNB Carboxyl sensor chip®, prepared standard curve covering the range of 1–50000 kIU/L, and established optimal measurement conditions. The validation included determination of the detection limit (LOD), functional sensitivity, linearity, precision, as well as the comparison with the results of the radioimmunoassay (RIA). The LOD and functional sensitivity were 4.2 kIU/L and 4.7 kIU/L, respectively. The method was linear in the range of 20–10000 kIU/L. The regression equation for comparison with RIA was CQCM = 1.0056 • CRIA – 24.2778, whereby no significant proportional or systematic difference was present. There was a good agreement with RIA in the classification of patients according to the clinical significance of the results. The developed method has advantages over currently available assays in terms of better LOQ, a higher upper limit of linearity, and precision. The characteristics of the developed method unambiguously show that the application of the QCM biosensors offers a highly reliable novel approach for the measurement of TgAb in human serum.",
publisher = "Elsevier",
journal = "Talanta",
title = "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors",
volume = "223",
pages = "121588",
doi = "10.1016/j.talanta.2020.121588"
}
Vrhovac, L., Šelemetjev, S. A., Vatić, S., Mitrović, A., Milošević, J., Lolić, A., Beletić, A. D.,& Polović, N.. (2021). Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta
Elsevier., 223, 121588.
https://doi.org/10.1016/j.talanta.2020.121588
Vrhovac L, Šelemetjev SA, Vatić S, Mitrović A, Milošević J, Lolić A, Beletić AD, Polović N. Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors. in Talanta. 2021;223:121588.
doi:10.1016/j.talanta.2020.121588 .
Vrhovac, Lidija, Šelemetjev, Sonja A., Vatić, Saša, Mitrović, Aleksandar, Milošević, Jelica, Lolić, Aleksandar, Beletić, Anđelo D., Polović, Natalija, "Novel approach to the measurement of antithyroglobulin antibodies in human serum – application of the quartz crystal microbalance sensors" in Talanta, 223 (2021):121588,
https://doi.org/10.1016/j.talanta.2020.121588 . .
1
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First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms

Dimitrijević, Marija V.; Mitić, Violeta D.; Nikolić, Jelena S.; Djordjevic, Aleksandra S.; Mutić, Jelena; Stankov-Jovanović, Vesna P.; Stojanovic, Gordana S.

(Wiley, 2019)

TY  - JOUR
AU  - Dimitrijević, Marija V.
AU  - Mitić, Violeta D.
AU  - Nikolić, Jelena S.
AU  - Djordjevic, Aleksandra S.
AU  - Mutić, Jelena
AU  - Stankov-Jovanović, Vesna P.
AU  - Stojanovic, Gordana S.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2871
AB  - The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms
VL  - 16
IS  - 2
SP  - 2
EP  - 11
DO  - 10.1002/cbdv.201800492
ER  - 
@article{
author = "Dimitrijević, Marija V. and Mitić, Violeta D. and Nikolić, Jelena S. and Djordjevic, Aleksandra S. and Mutić, Jelena and Stankov-Jovanović, Vesna P. and Stojanovic, Gordana S.",
year = "2019",
abstract = "The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms",
volume = "16",
number = "2",
pages = "2-11",
doi = "10.1002/cbdv.201800492"
}
Dimitrijević, M. V., Mitić, V. D., Nikolić, J. S., Djordjevic, A. S., Mutić, J., Stankov-Jovanović, V. P.,& Stojanovic, G. S.. (2019). First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity
Wiley., 16(2), 2-11.
https://doi.org/10.1002/cbdv.201800492
Dimitrijević MV, Mitić VD, Nikolić JS, Djordjevic AS, Mutić J, Stankov-Jovanović VP, Stojanovic GS. First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity. 2019;16(2):2-11.
doi:10.1002/cbdv.201800492 .
Dimitrijević, Marija V., Mitić, Violeta D., Nikolić, Jelena S., Djordjevic, Aleksandra S., Mutić, Jelena, Stankov-Jovanović, Vesna P., Stojanovic, Gordana S., "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms" in Chemistry and Biodiversity, 16, no. 2 (2019):2-11,
https://doi.org/10.1002/cbdv.201800492 . .
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10
9
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First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms

Dimitrijević, Marija V.; Mitić, Violeta D.; Nikolić, Jelena S.; Djordjevic, Aleksandra S.; Mutić, Jelena; Stankov-Jovanović, Vesna P.; Stojanovic, Gordana S.

(Wiley, 2019)

TY  - JOUR
AU  - Dimitrijević, Marija V.
AU  - Mitić, Violeta D.
AU  - Nikolić, Jelena S.
AU  - Djordjevic, Aleksandra S.
AU  - Mutić, Jelena
AU  - Stankov-Jovanović, Vesna P.
AU  - Stojanovic, Gordana S.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2844
AB  - The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms
VL  - 16
IS  - 2
SP  - 2
EP  - 11
DO  - 10.1002/cbdv.201800492
ER  - 
@article{
author = "Dimitrijević, Marija V. and Mitić, Violeta D. and Nikolić, Jelena S. and Djordjevic, Aleksandra S. and Mutić, Jelena and Stankov-Jovanović, Vesna P. and Stojanovic, Gordana S.",
year = "2019",
abstract = "The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms",
volume = "16",
number = "2",
pages = "2-11",
doi = "10.1002/cbdv.201800492"
}
Dimitrijević, M. V., Mitić, V. D., Nikolić, J. S., Djordjevic, A. S., Mutić, J., Stankov-Jovanović, V. P.,& Stojanovic, G. S.. (2019). First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity
Wiley., 16(2), 2-11.
https://doi.org/10.1002/cbdv.201800492
Dimitrijević MV, Mitić VD, Nikolić JS, Djordjevic AS, Mutić J, Stankov-Jovanović VP, Stojanovic GS. First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity. 2019;16(2):2-11.
doi:10.1002/cbdv.201800492 .
Dimitrijević, Marija V., Mitić, Violeta D., Nikolić, Jelena S., Djordjevic, Aleksandra S., Mutić, Jelena, Stankov-Jovanović, Vesna P., Stojanovic, Gordana S., "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms" in Chemistry and Biodiversity, 16, no. 2 (2019):2-11,
https://doi.org/10.1002/cbdv.201800492 . .
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10
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Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination

Antunović, Vesna R.; Tešanović, Slavna; Perušković, Danica S.; Stevanović, Nikola R.; Baošić, Rada; Mandić, Snezana D.; Lolić, Aleksandar

(Hindawi Ltd, London, 2018)

TY  - JOUR
AU  - Antunović, Vesna R.
AU  - Tešanović, Slavna
AU  - Perušković, Danica S.
AU  - Stevanović, Nikola R.
AU  - Baošić, Rada
AU  - Mandić, Snezana D.
AU  - Lolić, Aleksandar
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2122
AB  - The is work presents the development of a flow injection system for differential pulse amperometry (DPA) for diazepam determination in the presence of oxygen. The thin flow cell consisted of the bare glassy carbon electrode, reference silver/silver chloride, and stainless steel as the auxiliary electrode. Electrochemical reduction of diazepam (DZP) was characterised by cyclic voltammetry. Azomethine reduction peak was used for DZP quantification. The detector response was linear in the range 20-250 mu mol/dm(3) of diazepam, with a calculated detection limit of 3.83 mu g/cm(3). Intraday and interday precision were 1.53 and 10.8%, respectively. The method was applied on three beverage samples, energetic drink, and two different beer samples, and obtained recoveries were from 93.65 up to 104.96%. The throughoutput of the method was up to 90 analyses per hour.
PB  - Hindawi Ltd, London
T2  - Journal of Analytical Methods in Chemistry
T1  - Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination
DO  - 10.1155/2018/6121489
UR  - Kon_3453
ER  - 
@article{
author = "Antunović, Vesna R. and Tešanović, Slavna and Perušković, Danica S. and Stevanović, Nikola R. and Baošić, Rada and Mandić, Snezana D. and Lolić, Aleksandar",
year = "2018",
abstract = "The is work presents the development of a flow injection system for differential pulse amperometry (DPA) for diazepam determination in the presence of oxygen. The thin flow cell consisted of the bare glassy carbon electrode, reference silver/silver chloride, and stainless steel as the auxiliary electrode. Electrochemical reduction of diazepam (DZP) was characterised by cyclic voltammetry. Azomethine reduction peak was used for DZP quantification. The detector response was linear in the range 20-250 mu mol/dm(3) of diazepam, with a calculated detection limit of 3.83 mu g/cm(3). Intraday and interday precision were 1.53 and 10.8%, respectively. The method was applied on three beverage samples, energetic drink, and two different beer samples, and obtained recoveries were from 93.65 up to 104.96%. The throughoutput of the method was up to 90 analyses per hour.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Analytical Methods in Chemistry",
title = "Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination",
doi = "10.1155/2018/6121489",
url = "Kon_3453"
}
Antunović, V. R., Tešanović, S., Perušković, D. S., Stevanović, N. R., Baošić, R., Mandić, S. D.,& Lolić, A.. (2018). Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination. in Journal of Analytical Methods in Chemistry
Hindawi Ltd, London..
https://doi.org/10.1155/2018/6121489
Kon_3453
Antunović VR, Tešanović S, Perušković DS, Stevanović NR, Baošić R, Mandić SD, Lolić A. Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination. in Journal of Analytical Methods in Chemistry. 2018;.
doi:10.1155/2018/6121489
Kon_3453 .
Antunović, Vesna R., Tešanović, Slavna, Perušković, Danica S., Stevanović, Nikola R., Baošić, Rada, Mandić, Snezana D., Lolić, Aleksandar, "Development of a Flow Injection System for Differential Pulse Amperometry and Its Application for Diazepam Determination" in Journal of Analytical Methods in Chemistry (2018),
https://doi.org/10.1155/2018/6121489 .,
Kon_3453 .
4
3

Update on element content profiles in eleven wild edible mushrooms from family Boletaceae

Dimitrijević, Marija V.; Mitić, Violeta D.; Cvetković, Jelena S.; Stankov-Jovanović, Vesna P.; Mutić, Jelena; Nikolić-Mandić, Snežana D.

(Springer, New York, 2016)

TY  - JOUR
AU  - Dimitrijević, Marija V.
AU  - Mitić, Violeta D.
AU  - Cvetković, Jelena S.
AU  - Stankov-Jovanović, Vesna P.
AU  - Mutić, Jelena
AU  - Nikolić-Mandić, Snežana D.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2028
AB  - The aim of this study was to determine and evaluate the amounts of major elements (Ca, Fe, K, Na and P), essential trace elements (Cu, Zn, Fe and Mn) and some other trace metals (Ag, Al, Co, Ni, Cr, Sr, Se, Bi, Rb) in eleven species of wild-grown common edible mushrooms from family Boletaceae (Boletus appendiculatus, Boletus edulis, Boletus regius, Boletus fechtneri, Boletus impolitus, Boletus purpureus, Boletus rhodoxanthus, Leccinum crocipodium, Leccinum pseudoscaber, Xerocomellus chrysenteron, Xerocomus badius) from Serbia. The measurements of major elements (Ca, Fe, K, Na and P) were carried out by inductively coupled plasma optical emission spectrometer (ICP-OES), while analytical measurements of the rest of studied elements were performed using an inductively coupled plasma mass spectrometer (ICP-MS), after microwave digestion. The results showed that the element concentrations were species-dependent. Potassium and phosphorous concentrations were found to be greater than those of the other mineral constituents in all tested species. Multivariate analysis included principal component analysis (PCA) and hierarchical cluster analyses (HCA). HCA grouped mushrooms in three statistically significant clusters, while PCA indicated connection between analyzed metals. Also, this paper highlights the importance of essential and nonessential elements of human health and their daily intake.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Update on element content profiles in eleven wild edible mushrooms from family Boletaceae
VL  - 242
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1007/s00217-015-2512-0
UR  - Kon_2983
ER  - 
@article{
author = "Dimitrijević, Marija V. and Mitić, Violeta D. and Cvetković, Jelena S. and Stankov-Jovanović, Vesna P. and Mutić, Jelena and Nikolić-Mandić, Snežana D.",
year = "2016",
abstract = "The aim of this study was to determine and evaluate the amounts of major elements (Ca, Fe, K, Na and P), essential trace elements (Cu, Zn, Fe and Mn) and some other trace metals (Ag, Al, Co, Ni, Cr, Sr, Se, Bi, Rb) in eleven species of wild-grown common edible mushrooms from family Boletaceae (Boletus appendiculatus, Boletus edulis, Boletus regius, Boletus fechtneri, Boletus impolitus, Boletus purpureus, Boletus rhodoxanthus, Leccinum crocipodium, Leccinum pseudoscaber, Xerocomellus chrysenteron, Xerocomus badius) from Serbia. The measurements of major elements (Ca, Fe, K, Na and P) were carried out by inductively coupled plasma optical emission spectrometer (ICP-OES), while analytical measurements of the rest of studied elements were performed using an inductively coupled plasma mass spectrometer (ICP-MS), after microwave digestion. The results showed that the element concentrations were species-dependent. Potassium and phosphorous concentrations were found to be greater than those of the other mineral constituents in all tested species. Multivariate analysis included principal component analysis (PCA) and hierarchical cluster analyses (HCA). HCA grouped mushrooms in three statistically significant clusters, while PCA indicated connection between analyzed metals. Also, this paper highlights the importance of essential and nonessential elements of human health and their daily intake.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Update on element content profiles in eleven wild edible mushrooms from family Boletaceae",
volume = "242",
number = "1",
pages = "1-10",
doi = "10.1007/s00217-015-2512-0",
url = "Kon_2983"
}
Dimitrijević, M. V., Mitić, V. D., Cvetković, J. S., Stankov-Jovanović, V. P., Mutić, J.,& Nikolić-Mandić, S. D.. (2016). Update on element content profiles in eleven wild edible mushrooms from family Boletaceae. in European Food Research and Technology
Springer, New York., 242(1), 1-10.
https://doi.org/10.1007/s00217-015-2512-0
Kon_2983
Dimitrijević MV, Mitić VD, Cvetković JS, Stankov-Jovanović VP, Mutić J, Nikolić-Mandić SD. Update on element content profiles in eleven wild edible mushrooms from family Boletaceae. in European Food Research and Technology. 2016;242(1):1-10.
doi:10.1007/s00217-015-2512-0
Kon_2983 .
Dimitrijević, Marija V., Mitić, Violeta D., Cvetković, Jelena S., Stankov-Jovanović, Vesna P., Mutić, Jelena, Nikolić-Mandić, Snežana D., "Update on element content profiles in eleven wild edible mushrooms from family Boletaceae" in European Food Research and Technology, 242, no. 1 (2016):1-10,
https://doi.org/10.1007/s00217-015-2512-0 .,
Kon_2983 .
24
22
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The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System

Ilić, Marija; Haegel, Franz-Hubert; Pavelkić, Vesna; Zlatanović, Dragan; Nikolić-Mandić, Snežana D.; Lolić, Aleksandar; Nedić, Zoran

(Assoc Chemical Eng, Belgrade, 2016)

TY  - JOUR
AU  - Ilić, Marija
AU  - Haegel, Franz-Hubert
AU  - Pavelkić, Vesna
AU  - Zlatanović, Dragan
AU  - Nikolić-Mandić, Snežana D.
AU  - Lolić, Aleksandar
AU  - Nedić, Zoran
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1916
AB  - The influence of additives (alkyl polyglucoside, Glucopon 600 CS UP and alcohol ethoxylate C18E100) on the behavior of the water/toluene/Lutensol ON 50 (technical oxoalcohol, i-C10E5) microemulsion system as a function of temperature and composition has been investigated. The phase behavior of the microemulsions was determined by vertical sections through the Gibbs phase prism (fish-like phase diagrams). Alkyl polyglucoside shifts the one phase region to lower temperatures compared with water/toluene/Lutensol ON 50 mixtures. This is contrary to the expectation, considering the extreme hydrophilic nature of the sugar headgroup. The addition of hydrophilic alcohol ethoxylate (C18E100) to the water/toluene/Lutensol ON 50 system increases the solubilization capacity of the surfactant, even if the co-surfactant is used in small quantities, and shifts the one-phase region to higher temperature by a few degrees C.
PB  - Assoc Chemical Eng, Belgrade
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System
VL  - 22
IS  - 1
SP  - 27
EP  - 32
DO  - 10.2298/CICEQ141105015I
UR  - Kon_3035
ER  - 
@article{
author = "Ilić, Marija and Haegel, Franz-Hubert and Pavelkić, Vesna and Zlatanović, Dragan and Nikolić-Mandić, Snežana D. and Lolić, Aleksandar and Nedić, Zoran",
year = "2016",
abstract = "The influence of additives (alkyl polyglucoside, Glucopon 600 CS UP and alcohol ethoxylate C18E100) on the behavior of the water/toluene/Lutensol ON 50 (technical oxoalcohol, i-C10E5) microemulsion system as a function of temperature and composition has been investigated. The phase behavior of the microemulsions was determined by vertical sections through the Gibbs phase prism (fish-like phase diagrams). Alkyl polyglucoside shifts the one phase region to lower temperatures compared with water/toluene/Lutensol ON 50 mixtures. This is contrary to the expectation, considering the extreme hydrophilic nature of the sugar headgroup. The addition of hydrophilic alcohol ethoxylate (C18E100) to the water/toluene/Lutensol ON 50 system increases the solubilization capacity of the surfactant, even if the co-surfactant is used in small quantities, and shifts the one-phase region to higher temperature by a few degrees C.",
publisher = "Assoc Chemical Eng, Belgrade",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System",
volume = "22",
number = "1",
pages = "27-32",
doi = "10.2298/CICEQ141105015I",
url = "Kon_3035"
}
Ilić, M., Haegel, F., Pavelkić, V., Zlatanović, D., Nikolić-Mandić, S. D., Lolić, A.,& Nedić, Z.. (2016). The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System. in Chemical Industry and Chemical Engineering Quarterly / CICEQ
Assoc Chemical Eng, Belgrade., 22(1), 27-32.
https://doi.org/10.2298/CICEQ141105015I
Kon_3035
Ilić M, Haegel F, Pavelkić V, Zlatanović D, Nikolić-Mandić SD, Lolić A, Nedić Z. The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2016;22(1):27-32.
doi:10.2298/CICEQ141105015I
Kon_3035 .
Ilić, Marija, Haegel, Franz-Hubert, Pavelkić, Vesna, Zlatanović, Dragan, Nikolić-Mandić, Snežana D., Lolić, Aleksandar, Nedić, Zoran, "The Influence of Alkyl Polyglucosides (And Highly Ethoxylated Alcohol Boosters) on the Phase Behavior of a Water/Toluene/Technical Alkyl Polyethoxylate Microemulsion System" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22, no. 1 (2016):27-32,
https://doi.org/10.2298/CICEQ141105015I .,
Kon_3035 .
1
1
1

Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons

Tripković, T.; Charvy, C.; Alves, S.; Lolić, Aleksandar; Baošić, Rada; Nikolić-Mandić, Snežana D.; Tabet, J. C.

(Im Publications, W Sussex, 2015)

TY  - JOUR
AU  - Tripković, T.
AU  - Charvy, C.
AU  - Alves, S.
AU  - Lolić, Aleksandar
AU  - Baošić, Rada
AU  - Nikolić-Mandić, Snežana D.
AU  - Tabet, J. C.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1959
AB  - Proteomic approach in combination with mass spectrometry demonstrates a great potential for identification of proteinaceous materials in works of art. In this study we used a linear trap quadrupole Orbitrap (LTQ-Orbitraph a state-of-the-art mass spectrometer for parts per million accuracy analyses of peptides behind tryptic hydrolysis. After the efficiency of the proteomic method was confirmed for reference and model samples, micro-samples from historical paintings were for the first time analysed using this technique. Superior performances of the liquid chromatography-mass spectrometry approach using a LTQ-Orbitrap mass spectrometer allowed identification of egg yolk peptides in two samples from nineteenth-century Orthodox icons, indicating egg tempera as the painting technique. Accurate precursor ion masses, in the range of 2 ppm, and retention times of tryptic peptides strengthen protein identification. Additionally, in all historical samples the presence of animal glues suggested that the ground layer was likely bound using bovine collagen. Comparing to results acquired using matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry in our previous study, here we achieved higher ion scores and protein scores, better sequence coverage and more identified proteins. In fact, a combination of the two mass spectrometric techniques provided overlapping and complementary data, related to the detection of peptides with different physicochemical properties.
PB  - Im Publications, W Sussex
T2  - European Journal of Mass Spectrometry
T1  - Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons
VL  - 21
IS  - 4
SP  - 679
EP  - 692
DO  - 10.1255/ejms.1346
UR  - Kon_2914
ER  - 
@article{
author = "Tripković, T. and Charvy, C. and Alves, S. and Lolić, Aleksandar and Baošić, Rada and Nikolić-Mandić, Snežana D. and Tabet, J. C.",
year = "2015",
abstract = "Proteomic approach in combination with mass spectrometry demonstrates a great potential for identification of proteinaceous materials in works of art. In this study we used a linear trap quadrupole Orbitrap (LTQ-Orbitraph a state-of-the-art mass spectrometer for parts per million accuracy analyses of peptides behind tryptic hydrolysis. After the efficiency of the proteomic method was confirmed for reference and model samples, micro-samples from historical paintings were for the first time analysed using this technique. Superior performances of the liquid chromatography-mass spectrometry approach using a LTQ-Orbitrap mass spectrometer allowed identification of egg yolk peptides in two samples from nineteenth-century Orthodox icons, indicating egg tempera as the painting technique. Accurate precursor ion masses, in the range of 2 ppm, and retention times of tryptic peptides strengthen protein identification. Additionally, in all historical samples the presence of animal glues suggested that the ground layer was likely bound using bovine collagen. Comparing to results acquired using matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry in our previous study, here we achieved higher ion scores and protein scores, better sequence coverage and more identified proteins. In fact, a combination of the two mass spectrometric techniques provided overlapping and complementary data, related to the detection of peptides with different physicochemical properties.",
publisher = "Im Publications, W Sussex",
journal = "European Journal of Mass Spectrometry",
title = "Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons",
volume = "21",
number = "4",
pages = "679-692",
doi = "10.1255/ejms.1346",
url = "Kon_2914"
}
Tripković, T., Charvy, C., Alves, S., Lolić, A., Baošić, R., Nikolić-Mandić, S. D.,& Tabet, J. C.. (2015). Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons. in European Journal of Mass Spectrometry
Im Publications, W Sussex., 21(4), 679-692.
https://doi.org/10.1255/ejms.1346
Kon_2914
Tripković T, Charvy C, Alves S, Lolić A, Baošić R, Nikolić-Mandić SD, Tabet JC. Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons. in European Journal of Mass Spectrometry. 2015;21(4):679-692.
doi:10.1255/ejms.1346
Kon_2914 .
Tripković, T., Charvy, C., Alves, S., Lolić, Aleksandar, Baošić, Rada, Nikolić-Mandić, Snežana D., Tabet, J. C., "Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century Orthodox icons" in European Journal of Mass Spectrometry, 21, no. 4 (2015):679-692,
https://doi.org/10.1255/ejms.1346 .,
Kon_2914 .
6
5
5

Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry

Tripković, T.; Charvy, C.; Alves, S.; Lolić, Aleksandar; Baošić, Rada; Nikolić-Mandić, Snežana D.; Tabet, J. C.

(Elsevier Science Bv, Amsterdam, 2013)

TY  - JOUR
AU  - Tripković, T.
AU  - Charvy, C.
AU  - Alves, S.
AU  - Lolić, Aleksandar
AU  - Baošić, Rada
AU  - Nikolić-Mandić, Snežana D.
AU  - Tabet, J. C.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1366
AB  - Aim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples.
PB  - Elsevier Science Bv, Amsterdam
T2  - Talanta
T1  - Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry
VL  - 113
SP  - 49
EP  - 61
DO  - 10.1016/j.talanta.2013.03.071
UR  - Kon_2486
ER  - 
@article{
author = "Tripković, T. and Charvy, C. and Alves, S. and Lolić, Aleksandar and Baošić, Rada and Nikolić-Mandić, Snežana D. and Tabet, J. C.",
year = "2013",
abstract = "Aim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry",
volume = "113",
pages = "49-61",
doi = "10.1016/j.talanta.2013.03.071",
url = "Kon_2486"
}
Tripković, T., Charvy, C., Alves, S., Lolić, A., Baošić, R., Nikolić-Mandić, S. D.,& Tabet, J. C.. (2013). Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry. in Talanta
Elsevier Science Bv, Amsterdam., 113, 49-61.
https://doi.org/10.1016/j.talanta.2013.03.071
Kon_2486
Tripković T, Charvy C, Alves S, Lolić A, Baošić R, Nikolić-Mandić SD, Tabet JC. Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry. in Talanta. 2013;113:49-61.
doi:10.1016/j.talanta.2013.03.071
Kon_2486 .
Tripković, T., Charvy, C., Alves, S., Lolić, Aleksandar, Baošić, Rada, Nikolić-Mandić, Snežana D., Tabet, J. C., "Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry" in Talanta, 113 (2013):49-61,
https://doi.org/10.1016/j.talanta.2013.03.071 .,
Kon_2486 .
23
22
22

Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071

Tripković, T.; Charvy, C.; Alves, S.; Lolić, Aleksandar; Baošić, Rada; Nikolić-Mandić, Snežana D.; Tabet, J. C.

(Elsevier Science Bv, Amsterdam, 2013)

TY  - DATA
AU  - Tripković, T.
AU  - Charvy, C.
AU  - Alves, S.
AU  - Lolić, Aleksandar
AU  - Baošić, Rada
AU  - Nikolić-Mandić, Snežana D.
AU  - Tabet, J. C.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3500
PB  - Elsevier Science Bv, Amsterdam
T2  - Talanta
T1  - Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071
ER  - 
@misc{
author = "Tripković, T. and Charvy, C. and Alves, S. and Lolić, Aleksandar and Baošić, Rada and Nikolić-Mandić, Snežana D. and Tabet, J. C.",
year = "2013",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071"
}
Tripković, T., Charvy, C., Alves, S., Lolić, A., Baošić, R., Nikolić-Mandić, S. D.,& Tabet, J. C.. (2013). Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071. in Talanta
Elsevier Science Bv, Amsterdam..
Tripković T, Charvy C, Alves S, Lolić A, Baošić R, Nikolić-Mandić SD, Tabet JC. Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071. in Talanta. 2013;..
Tripković, T., Charvy, C., Alves, S., Lolić, Aleksandar, Baošić, Rada, Nikolić-Mandić, Snežana D., Tabet, J. C., "Supplementary data for article: Tripkovic, T.; Charvy, C.; Alves, S.; Lolić, A.; Baošić, R.; Nikolić-Mandić, S. D.; Tabet, J. C. Identification of Protein Binders in Artworks by MALDI-TOF/TOF Tandem Mass Spectrometry. Talanta 2013, 113, 49–61. https://doi.org/10.1016/j.talanta.2013.03.071" in Talanta (2013).

Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples

Lolić, Aleksandar; Tripkovic, Tatjana; Baošić, Rada; Nikolić-Mandić, Snežana D.; Stanimirović, Bojana

(Serbian Chemical Soc, Belgrade, 2012)

TY  - JOUR
AU  - Lolić, Aleksandar
AU  - Tripkovic, Tatjana
AU  - Baošić, Rada
AU  - Nikolić-Mandić, Snežana D.
AU  - Stanimirović, Bojana
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1569
AB  - A gas-diffusion flow injection method with amperometric detection for the indirect determination of copper on a silver electrode was developed. The flow through system was equipped with two injection valves and a gas-diffusion unit. In the first step, a signal of a cyanide solution was recorded. In the subsequent step, the signal of cyanide in the presence of copper was measured. Interferences (Cd(II), Co(II), Ag(I), Ni(II), Fe(III), Hg(II) and Zn(II)) were investigated and successfully removed. The calibration graph was linear in the range 1-90 mu mol dm(-3) of copper with a correlation coefficient of 0.993. The regression equation is I = (0.0455 +/- 0.0015)c + (0.4611 +/- 0.0671), where I is the relative signal decrease in mu A and c is concentration in mu mol dm(-3). Relative standard deviation for six consecutive injections of 30 mu mol dm(-3) copper(II) was 1.47 % and for 1 mu mol dm(-3) copper(II), it was 3.40 %. The detection limit, calculated as 3 s/m (where s is a standard deviation of nine measurement of a reagent blank and m is the slope of the calibration curve), was 0.32 mu mol dm(-3), which corresponds to 2.44 ng of copper(II) (the loop volume was 0.12 cm(3)). The method enables 60 analyses per hour and it was successfully applied for the determination of copper in drinking water samples.
AB  - Cilj ovog rada bio je razvoj gasno-difuzione protočno injekcione metode sa amperometrijskom detekcijom za indirektno određivanje bakra na srebrnoj elektrodi. Protočni sistem je opremljen sa dva injekciona ventila i gasno-difuzionom jedinicom. U prvom koraku snima se signal rastvora cijanida, a u drugom snima se signal pri istovremenom ubrizgavanju rastvora cijanida i rastvora bakra. Smetnje od Cd(II), Co(II), Ag(I), Ni(II), Fe(III), Hg(II) i Zn(II) su ispitane i uspešno uklonjene. Kalibraciona kriva je linearna u opsegu 1-90 μmol dm-3 bakra, koeficijent korelacije je 0,993, a jednačina prave I = (0,0455±0,0015)c + (0,4611±0,0671), gde je I razlika visine signala u μA, a c je koncentracija u μmol dm-3. Relativna standardna devijacija za šest uzastopnih ubrizgavanja rastvora bakra koncentracije 30 μmol dm-3 je 1,47 %, a za 1 μmol dm-3 rastvor Cu(II) je 3,40 %. Limit detekcije, izračunat kao 3s/m (gde je s standardna devijacija devet merenja slepe probe i m je nagib kalibracione prave), je 0,32 μmol dm-3, što odgovara 2,44 ng Cu(II) (zapremina petlje za uzorak je 0,12 cm3). Metoda omogućava 60 analiza po jednom satu i uspešno je primenjena za određivanje bakra u pijaćoj vodi.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples
T1  - Razvoj protočne injekcione metode za indirektno određivanje bakra sa amperometrijskom detekcijom u pijaćoj vodi
VL  - 77
IS  - 11
SP  - 1641
EP  - 1647
DO  - 10.2298/JSC120616090L
UR  - Kon_2400
ER  - 
@article{
author = "Lolić, Aleksandar and Tripkovic, Tatjana and Baošić, Rada and Nikolić-Mandić, Snežana D. and Stanimirović, Bojana",
year = "2012",
abstract = "A gas-diffusion flow injection method with amperometric detection for the indirect determination of copper on a silver electrode was developed. The flow through system was equipped with two injection valves and a gas-diffusion unit. In the first step, a signal of a cyanide solution was recorded. In the subsequent step, the signal of cyanide in the presence of copper was measured. Interferences (Cd(II), Co(II), Ag(I), Ni(II), Fe(III), Hg(II) and Zn(II)) were investigated and successfully removed. The calibration graph was linear in the range 1-90 mu mol dm(-3) of copper with a correlation coefficient of 0.993. The regression equation is I = (0.0455 +/- 0.0015)c + (0.4611 +/- 0.0671), where I is the relative signal decrease in mu A and c is concentration in mu mol dm(-3). Relative standard deviation for six consecutive injections of 30 mu mol dm(-3) copper(II) was 1.47 % and for 1 mu mol dm(-3) copper(II), it was 3.40 %. The detection limit, calculated as 3 s/m (where s is a standard deviation of nine measurement of a reagent blank and m is the slope of the calibration curve), was 0.32 mu mol dm(-3), which corresponds to 2.44 ng of copper(II) (the loop volume was 0.12 cm(3)). The method enables 60 analyses per hour and it was successfully applied for the determination of copper in drinking water samples., Cilj ovog rada bio je razvoj gasno-difuzione protočno injekcione metode sa amperometrijskom detekcijom za indirektno određivanje bakra na srebrnoj elektrodi. Protočni sistem je opremljen sa dva injekciona ventila i gasno-difuzionom jedinicom. U prvom koraku snima se signal rastvora cijanida, a u drugom snima se signal pri istovremenom ubrizgavanju rastvora cijanida i rastvora bakra. Smetnje od Cd(II), Co(II), Ag(I), Ni(II), Fe(III), Hg(II) i Zn(II) su ispitane i uspešno uklonjene. Kalibraciona kriva je linearna u opsegu 1-90 μmol dm-3 bakra, koeficijent korelacije je 0,993, a jednačina prave I = (0,0455±0,0015)c + (0,4611±0,0671), gde je I razlika visine signala u μA, a c je koncentracija u μmol dm-3. Relativna standardna devijacija za šest uzastopnih ubrizgavanja rastvora bakra koncentracije 30 μmol dm-3 je 1,47 %, a za 1 μmol dm-3 rastvor Cu(II) je 3,40 %. Limit detekcije, izračunat kao 3s/m (gde je s standardna devijacija devet merenja slepe probe i m je nagib kalibracione prave), je 0,32 μmol dm-3, što odgovara 2,44 ng Cu(II) (zapremina petlje za uzorak je 0,12 cm3). Metoda omogućava 60 analiza po jednom satu i uspešno je primenjena za određivanje bakra u pijaćoj vodi.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples, Razvoj protočne injekcione metode za indirektno određivanje bakra sa amperometrijskom detekcijom u pijaćoj vodi",
volume = "77",
number = "11",
pages = "1641-1647",
doi = "10.2298/JSC120616090L",
url = "Kon_2400"
}
Lolić, A., Tripkovic, T., Baošić, R., Nikolić-Mandić, S. D.,& Stanimirović, B.. (2012). Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 77(11), 1641-1647.
https://doi.org/10.2298/JSC120616090L
Kon_2400
Lolić A, Tripkovic T, Baošić R, Nikolić-Mandić SD, Stanimirović B. Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples. in Journal of the Serbian Chemical Society. 2012;77(11):1641-1647.
doi:10.2298/JSC120616090L
Kon_2400 .
Lolić, Aleksandar, Tripkovic, Tatjana, Baošić, Rada, Nikolić-Mandić, Snežana D., Stanimirović, Bojana, "Development of a flow injection method with amperometric detection for the indirect determination of copper in drinking water samples" in Journal of the Serbian Chemical Society, 77, no. 11 (2012):1641-1647,
https://doi.org/10.2298/JSC120616090L .,
Kon_2400 .
1
2
2

Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase

Stankov-Jovanović, Vesna P.; Mitić, Violeta D.; Ilić, Marija D.; Mandić, Ljuba M.; Nikolić-Mandić, Snežana D.

(Assoc Chemists & Chemical Engineers Of Serbia, Belgrade, 2012)

TY  - JOUR
AU  - Stankov-Jovanović, Vesna P.
AU  - Mitić, Violeta D.
AU  - Ilić, Marija D.
AU  - Mandić, Ljuba M.
AU  - Nikolić-Mandić, Snežana D.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1598
AB  - Propranolol, a widely used beta-blocker, inhibits the hydrolysis reaction of enzyme cholinesterase. Measurements of the difference in rate of hydrolysis rate between uninhibited and inhibited reactions allow the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (K-M, 0.326-0.330 mmol/L; V-max, 40.0-43.0 mu mol/Lmin). The inhibition type was recognized as competitive and the inhibition constant, Ki, was determined to be 22.60 mu mol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 mu mol/L, respectively. Accuracy and precision of proposed methods were tested. The proposed method showed good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications.
AB  - Za propranolol, često propisivani neselektivni beta blokator, utvrđeno je da inhibira reakciju enzimske hidrolize butiriltioholin-jodida, koja je katalizovana serumskom holinesterazom. Merenjem razlike u brzini osnovne i inhibitorske reakcije hidrolize u prisustvu propranolola kao inhibitora, moguće je razviti kinetičku metodu za određivanje propranolola. Oba sistema, enzim-supstrat-hromogen kao i enzim-supstrat-hromogen-inhibitor, okarakterisani su biohemijskim kinetičkim parametrima (KM, 0,326-0,330 mmol/L; Vmax, 40-42,99 μmol/L min), inhibicija je definisana kao kompetitivna i određena je konstanta inhibicije 22,60 μmol/L. Da bi se u potpunosti iskoristile sve mogućnosti predložene metode u pogledu osetljivosti, tačnosti, preciznosti i selektivnosti, optimizovani su reakcioni uslovi. Konstruisana je kalibraciona prava, izračunata odgovarajuća jednačina i određeni granica detekcije i kvantifikacije i to 0,004 i 0,0136 μmol/L, redom. Tačnost i preciznost predložene metode su ispitane za tri koncentracije propranolola u oblasti kalibracione prave (0,082-21,120 μmol/L) u pet ponavljanja. Takođe, ispitan je uticaj većeg broja supstanci koje se mogu naći u uzorku na brzinu reakcije. Optimizovana metoda je primenjena za određivanje propranolola u farmaceutskim preparatima. Tačnost predložene metode je ispitana primenom metode standardnog dodatka. Predložena metoda ima dobru osetljivost, selektivnost, jednostavna je i brza, i nadasve lako dostupna, i na taj način primenljiva u velikom broju laboratorija.
PB  - Assoc Chemists & Chemical Engineers Of Serbia, Belgrade
T2  - Hemijska industrija
T1  - Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase
T1  - Enzimska kinetička metoda za određivanje propranolol-hidrohlorida u farmaceutskim preparatima zasnovana na njegovom inhibitorskom delovanju na holinesterazu
VL  - 66
IS  - 5
SP  - 677
EP  - 684
DO  - 10.2298/HEMIND120128032S
UR  - Kon_2429
ER  - 
@article{
author = "Stankov-Jovanović, Vesna P. and Mitić, Violeta D. and Ilić, Marija D. and Mandić, Ljuba M. and Nikolić-Mandić, Snežana D.",
year = "2012",
abstract = "Propranolol, a widely used beta-blocker, inhibits the hydrolysis reaction of enzyme cholinesterase. Measurements of the difference in rate of hydrolysis rate between uninhibited and inhibited reactions allow the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (K-M, 0.326-0.330 mmol/L; V-max, 40.0-43.0 mu mol/Lmin). The inhibition type was recognized as competitive and the inhibition constant, Ki, was determined to be 22.60 mu mol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 mu mol/L, respectively. Accuracy and precision of proposed methods were tested. The proposed method showed good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications., Za propranolol, često propisivani neselektivni beta blokator, utvrđeno je da inhibira reakciju enzimske hidrolize butiriltioholin-jodida, koja je katalizovana serumskom holinesterazom. Merenjem razlike u brzini osnovne i inhibitorske reakcije hidrolize u prisustvu propranolola kao inhibitora, moguće je razviti kinetičku metodu za određivanje propranolola. Oba sistema, enzim-supstrat-hromogen kao i enzim-supstrat-hromogen-inhibitor, okarakterisani su biohemijskim kinetičkim parametrima (KM, 0,326-0,330 mmol/L; Vmax, 40-42,99 μmol/L min), inhibicija je definisana kao kompetitivna i određena je konstanta inhibicije 22,60 μmol/L. Da bi se u potpunosti iskoristile sve mogućnosti predložene metode u pogledu osetljivosti, tačnosti, preciznosti i selektivnosti, optimizovani su reakcioni uslovi. Konstruisana je kalibraciona prava, izračunata odgovarajuća jednačina i određeni granica detekcije i kvantifikacije i to 0,004 i 0,0136 μmol/L, redom. Tačnost i preciznost predložene metode su ispitane za tri koncentracije propranolola u oblasti kalibracione prave (0,082-21,120 μmol/L) u pet ponavljanja. Takođe, ispitan je uticaj većeg broja supstanci koje se mogu naći u uzorku na brzinu reakcije. Optimizovana metoda je primenjena za određivanje propranolola u farmaceutskim preparatima. Tačnost predložene metode je ispitana primenom metode standardnog dodatka. Predložena metoda ima dobru osetljivost, selektivnost, jednostavna je i brza, i nadasve lako dostupna, i na taj način primenljiva u velikom broju laboratorija.",
publisher = "Assoc Chemists & Chemical Engineers Of Serbia, Belgrade",
journal = "Hemijska industrija",
title = "Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase, Enzimska kinetička metoda za određivanje propranolol-hidrohlorida u farmaceutskim preparatima zasnovana na njegovom inhibitorskom delovanju na holinesterazu",
volume = "66",
number = "5",
pages = "677-684",
doi = "10.2298/HEMIND120128032S",
url = "Kon_2429"
}
Stankov-Jovanović, V. P., Mitić, V. D., Ilić, M. D., Mandić, L. M.,& Nikolić-Mandić, S. D.. (2012). Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase. in Hemijska industrija
Assoc Chemists & Chemical Engineers Of Serbia, Belgrade., 66(5), 677-684.
https://doi.org/10.2298/HEMIND120128032S
Kon_2429
Stankov-Jovanović VP, Mitić VD, Ilić MD, Mandić LM, Nikolić-Mandić SD. Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase. in Hemijska industrija. 2012;66(5):677-684.
doi:10.2298/HEMIND120128032S
Kon_2429 .
Stankov-Jovanović, Vesna P., Mitić, Violeta D., Ilić, Marija D., Mandić, Ljuba M., Nikolić-Mandić, Snežana D., "Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase" in Hemijska industrija, 66, no. 5 (2012):677-684,
https://doi.org/10.2298/HEMIND120128032S .,
Kon_2429 .
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