TY  - JOUR
AU  - Nešić, Jelena
AU  - Rtimi, Sami
AU  - Laub, Daniele
AU  - Roglić, Goran
AU  - Pulgarin, Cesar
AU  - Kiwi, John
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1897
AB  - This study presents new evidence for the events leading to Escherichia coli reduction in the absence of light irradiation on TiO2-polyester (from now on TiO2-PES. By transmission electron microscopy (TEM) the diffusion of TiO2 NP's aggregates with the E. coli outer lipo-polyssacharide (LPS) layer is shown to be a prerequisite for the loss of bacterial cultivability. Within 30 min in the dark the TiO2 aggregates interact with E. coli cell wall leading within 120 min to the complete loss of bacterial cultivability on a TiO2-PES 5% TiO2 sample. The bacterial reduction was observed to increase with a higher TiO2 loading on the PES up to 5%. Bacterial disinfection on TiO2-PES in the dark was slower compared to the runs under low intensity simulated sunlight light irradiation. The interaction between the TiO2 aggregates and the E. coli cell wall is discussed in terms of the competition between the TiO2 units collapsing to form TiO2-aggregates at a physiologic pH-value followed by the electrostatic interaction with the bacteria surface. TiO2-PES samples were able to carry repetitive bacterial inactivation. This presents a potential for practical applications. X-ray photoelectron spectroscopy (XPS) evidence was found for the reduction of Ti4+ to Ti3+ contributing to redox interactions between TiO2-PES and the bacterial cell wall. Insight is provided into the mechanism of interaction between the E. coli cell wall and TiO2 NP's. The properties of the TiO2-PES surface like percentage atomic concentration, TiO2-loading, optical absorption, surface charge and crystallographic phases are reported in this study. (C) 2014 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - New evidence for TiO2 uniform surfaces leading to complete bacterial reduction in the dark: Critical issues
VL  - 123
SP  - 593
EP  - 599
DO  - 10.1016/j.colsurfb.2014.09.060
ER  - 

@article{
author = "Nešić, Jelena and Rtimi, Sami and Laub, Daniele and Roglić, Goran and Pulgarin, Cesar and Kiwi, John",
year = "2014",
abstract = "This study presents new evidence for the events leading to Escherichia coli reduction in the absence of light irradiation on TiO2-polyester (from now on TiO2-PES. By transmission electron microscopy (TEM) the diffusion of TiO2 NP's aggregates with the E. coli outer lipo-polyssacharide (LPS) layer is shown to be a prerequisite for the loss of bacterial cultivability. Within 30 min in the dark the TiO2 aggregates interact with E. coli cell wall leading within 120 min to the complete loss of bacterial cultivability on a TiO2-PES 5% TiO2 sample. The bacterial reduction was observed to increase with a higher TiO2 loading on the PES up to 5%. Bacterial disinfection on TiO2-PES in the dark was slower compared to the runs under low intensity simulated sunlight light irradiation. The interaction between the TiO2 aggregates and the E. coli cell wall is discussed in terms of the competition between the TiO2 units collapsing to form TiO2-aggregates at a physiologic pH-value followed by the electrostatic interaction with the bacteria surface. TiO2-PES samples were able to carry repetitive bacterial inactivation. This presents a potential for practical applications. X-ray photoelectron spectroscopy (XPS) evidence was found for the reduction of Ti4+ to Ti3+ contributing to redox interactions between TiO2-PES and the bacterial cell wall. Insight is provided into the mechanism of interaction between the E. coli cell wall and TiO2 NP's. The properties of the TiO2-PES surface like percentage atomic concentration, TiO2-loading, optical absorption, surface charge and crystallographic phases are reported in this study. (C) 2014 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "New evidence for TiO2 uniform surfaces leading to complete bacterial reduction in the dark: Critical issues",
volume = "123",
pages = "593-599",
doi = "10.1016/j.colsurfb.2014.09.060"
}

Nešić, J., Rtimi, S., Laub, D., Roglić, G., Pulgarin, C.,& Kiwi, J.. (2014). New evidence for TiO2 uniform surfaces leading to complete bacterial reduction in the dark: Critical issues. in Colloids and Surfaces. B: Biointerfaces
Elsevier Science Bv, Amsterdam., 123, 593-599.
https://doi.org/10.1016/j.colsurfb.2014.09.060

Nešić J, Rtimi S, Laub D, Roglić G, Pulgarin C, Kiwi J. New evidence for TiO2 uniform surfaces leading to complete bacterial reduction in the dark: Critical issues. in Colloids and Surfaces. B: Biointerfaces. 2014;123:593-599.
doi:10.1016/j.colsurfb.2014.09.060 .

Nešić, Jelena, Rtimi, Sami, Laub, Daniele, Roglić, Goran, Pulgarin, Cesar, Kiwi, John, "New evidence for TiO2 uniform surfaces leading to complete bacterial reduction in the dark: Critical issues" in Colloids and Surfaces. B: Biointerfaces, 123 (2014):593-599,
https://doi.org/10.1016/j.colsurfb.2014.09.060 . .