TY  - DATA
AU  - Penjišević, Jelena 
AU  - Šukalović, Vladimir 
AU  - Dukić-Stefanović, Slađana
AU  - Deuther-Conrad, Winnie
AU  - Andrić, Deana 
AU  - Kostić-Rajačić, Slađana
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5845
AB  - Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.
PB  - Elsevier
T2  - Arabian Journal of Chemistry
T1  - Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636
VL  - 16
IS  - 4
SP  - 104636
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5845
ER  - 

@misc{
author = "Penjišević, Jelena  and Šukalović, Vladimir  and Dukić-Stefanović, Slađana and Deuther-Conrad, Winnie and Andrić, Deana  and Kostić-Rajačić, Slađana",
abstract = "Serotonin receptors modulate numerous behavioral and neuropsychological processes. Therefore, they are the target for the action of many drugs, such as antipsychotics, antidepressants, antiemetics, migraine remedies, and many others. The 5-HT1A receptors have been involved in the pathogenesis and treatment of anxiety and depression and represent a promising target for new drugs with reduced extrapyramidal side effects. In most antidepressants, a piperazine-based structural motif can be identified as a common moiety. Here we describe the synthesis, pharmacological, and in silico characterization of a novel arylpiperazines series with excellent 5-HT1A affinity. The final compounds, 4a, 8a, and 8b, were selected according to predictions of in silico pharmacokinetics, docking analysis, and molecular dynamics in conjunction with physical properties, and metabolic stability. The accentuated molecules could serve as a lead compound for developing 5-HT1A drug-like molecules for depression treatment.",
publisher = "Elsevier",
journal = "Arabian Journal of Chemistry",
title = "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636",
volume = "16",
number = "4",
pages = "104636",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5845"
}

Penjišević, J., Šukalović, V., Dukić-Stefanović, S., Deuther-Conrad, W., Andrić, D.,& Kostić-Rajačić, S..Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry
Elsevier., 16(4), 104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845

Penjišević J, Šukalović V, Dukić-Stefanović S, Deuther-Conrad W, Andrić D, Kostić-Rajačić S. Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636. in Arabian Journal of Chemistry.16(4):104636.
https://hdl.handle.net/21.15107/rcub_cherry_5845 .

Penjišević, Jelena , Šukalović, Vladimir , Dukić-Stefanović, Slađana, Deuther-Conrad, Winnie, Andrić, Deana , Kostić-Rajačić, Slađana, "Supplementary material for: Penjišević, J. Z., Šukalović, V. B., Dukic-Stefanovic, S., Deuther-Conrad, W., Andrić, D. B.,& Kostić-Rajačić, S. V.. (2023). Synthesis of novel 5-HT1A arylpiperazine ligands: Binding data and computer-aided analysis of pharmacological potency. in Arabian Journal of Chemistry Elsevier., 16(4), 104636. https://doi.org/10.1016/j.arabjc.2023.104636" in Arabian Journal of Chemistry, 16, no. 4:104636,
https://hdl.handle.net/21.15107/rcub_cherry_5845 .

TY  - JOUR
AU  - Vukićević, Emilija
AU  - Isailović, Jelena
AU  - Gajica, Gordana
AU  - Antić, Vesna
AU  - Jovančićević, Branimir
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6620
AB  - The stalks left after harvesting corn, tomatoes, and tobacco have nofurther use and are usually burned on agricultural land. In our work samples ofthis waste were collected and pyrolyzed at 400 ℃ for 30 min in a nitrogenatmosphere. The solid residue (biochar) obtained by pyrolysis was analyzed,and the results were compared with widely used solid fuels such as wood, coal,coke and charcoal. The heat values of biochar from tomato, tobacco, corn ZP6263, and corn BC 398 stalks were 24.12, 23.09, 26.24 and 25.78 MJ kg-1, respectively.These values are significantly higher than the heat value of wood,which is about 12.50 MJ kg-1. The ash content of biochar was 12–20 %, whichis consistent with the ash content of solid fuels. No heavy metals were found inbiochar samples. The results show that biochar obtained from the pyrolysis ofagricultural waste, such as tomatoes, tobacco and corn stalks, has good potentialfor use as a solid fuel.
AB  - Стабљике које остају након жетве кукуруза и дувана, као и брања парадајза,немају даљу употребу и обично се спаљују на пољопривредном земљишту. Унашем раду узорци овог отпада су прикупљени и пиролизовани на 400 ℃ у атмо-сфери азота током 30 min. Анализиран је чврсти остатак (биоугаљ) добијен пиро-лизом, а резултати су упоређени са широко коришћеним конвенционалним чврс-тим горивима као што су дрво, угаљ, кокс и дрвени угаљ. Калоријске вредностибиоугља из стабљика парадајза, дувана, кукуруза ZP 6263 и кукуруза BC 398 билесу 24,12, 23,09, 26,24 и 25,78 МЈ kg-1. Ове вредности су знатно веће од калоријскевредности дрвета која износи око 12,50 МЈ kg-1. Садржај пепела у биоугљу је био12–20 %, што је у складу са садржајем пепела код чврстих горива. У узорцима био-угља нису пронађени тешки метали. Резултати показују да биоугаљ добијен пиро-лизом пољопривредног отпада, као што су стабљике парадајза, дувана и кукуруза,има добар потенцијал за употребу као чврсто гориво.
PB  - Srpsko hemijsko društvo
T2  - Journal of the Serbian Chemical Society
T1  - Biochar from agricultural biomass: Green material as an ecological alternative to solid fossil fuels
T1  - Биоугаљ из пољопривредне биомасе: зелени материјал као еколошка алтернатива чврстим фосилним горивима
VL  - 89
IS  - 6
SP  - 907
EP  - 919
DO  - 10.2298/JSC240126048V
ER  - 

@article{
author = "Vukićević, Emilija and Isailović, Jelena and Gajica, Gordana and Antić, Vesna and Jovančićević, Branimir",
year = "2024",
abstract = "The stalks left after harvesting corn, tomatoes, and tobacco have nofurther use and are usually burned on agricultural land. In our work samples ofthis waste were collected and pyrolyzed at 400 ℃ for 30 min in a nitrogenatmosphere. The solid residue (biochar) obtained by pyrolysis was analyzed,and the results were compared with widely used solid fuels such as wood, coal,coke and charcoal. The heat values of biochar from tomato, tobacco, corn ZP6263, and corn BC 398 stalks were 24.12, 23.09, 26.24 and 25.78 MJ kg-1, respectively.These values are significantly higher than the heat value of wood,which is about 12.50 MJ kg-1. The ash content of biochar was 12–20 %, whichis consistent with the ash content of solid fuels. No heavy metals were found inbiochar samples. The results show that biochar obtained from the pyrolysis ofagricultural waste, such as tomatoes, tobacco and corn stalks, has good potentialfor use as a solid fuel., Стабљике које остају након жетве кукуруза и дувана, као и брања парадајза,немају даљу употребу и обично се спаљују на пољопривредном земљишту. Унашем раду узорци овог отпада су прикупљени и пиролизовани на 400 ℃ у атмо-сфери азота током 30 min. Анализиран је чврсти остатак (биоугаљ) добијен пиро-лизом, а резултати су упоређени са широко коришћеним конвенционалним чврс-тим горивима као што су дрво, угаљ, кокс и дрвени угаљ. Калоријске вредностибиоугља из стабљика парадајза, дувана, кукуруза ZP 6263 и кукуруза BC 398 билесу 24,12, 23,09, 26,24 и 25,78 МЈ kg-1. Ове вредности су знатно веће од калоријскевредности дрвета која износи око 12,50 МЈ kg-1. Садржај пепела у биоугљу је био12–20 %, што је у складу са садржајем пепела код чврстих горива. У узорцима био-угља нису пронађени тешки метали. Резултати показују да биоугаљ добијен пиро-лизом пољопривредног отпада, као што су стабљике парадајза, дувана и кукуруза,има добар потенцијал за употребу као чврсто гориво.",
publisher = "Srpsko hemijsko društvo",
journal = "Journal of the Serbian Chemical Society",
title = "Biochar from agricultural biomass: Green material as an ecological alternative to solid fossil fuels, Биоугаљ из пољопривредне биомасе: зелени материјал као еколошка алтернатива чврстим фосилним горивима",
volume = "89",
number = "6",
pages = "907-919",
doi = "10.2298/JSC240126048V"
}

Vukićević, E., Isailović, J., Gajica, G., Antić, V.,& Jovančićević, B.. (2024). Biochar from agricultural biomass: Green material as an ecological alternative to solid fossil fuels. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo., 89(6), 907-919.
https://doi.org/10.2298/JSC240126048V

Vukićević E, Isailović J, Gajica G, Antić V, Jovančićević B. Biochar from agricultural biomass: Green material as an ecological alternative to solid fossil fuels. in Journal of the Serbian Chemical Society. 2024;89(6):907-919.
doi:10.2298/JSC240126048V .

Vukićević, Emilija, Isailović, Jelena, Gajica, Gordana, Antić, Vesna, Jovančićević, Branimir, "Biochar from agricultural biomass: Green material as an ecological alternative to solid fossil fuels" in Journal of the Serbian Chemical Society, 89, no. 6 (2024):907-919,
https://doi.org/10.2298/JSC240126048V . .

TY  - JOUR
AU  - Matošević, Ana
AU  - Bartolić, Marija
AU  - Maraković, Nikola
AU  - Zandona, Antonio
AU  - Petrić, Rajo
AU  - Opsenica, Dejan
AU  - Bosak, Anita
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6617
AB  - Alzheimer’s disease is age-related multifactorial neurodegenerative disease manifested by gradual loss of memory, cognitive decline and changes in personality. Due to rapid and continuous growth of its prevalence, the treatment of Alzheimer’s disease calls for development of new and efficacies drugs, especially those that could be able to simultaneously act on more than one of possible targets of action. Aminoquinolines have proven to be a highly promising structural scaffold in the design of such a drug as cholinesterases and β-secretase 1 inhibitors. In this study, we synthesised twenty-two new 4-aminoquinolines with different halogen atom and its position in the terminal N-benzyl group or with a trifluoromethyl or a chlorine as C(7)-substituents on the quinoline moiety. All compounds were evaluated as multi-target-directedligands by determining their inhibition potency towards human acetylcholinesterase, butyrylcholinesterase and β-secretase 1. All of the tested derivatives were very potent inhibitors of human acetylcholinesterase and butyrylcholinesterase with inhibition constants (Ki) in the nM to low μM range. Most were estimated to be able to cross the blood–brain barrier by passive transport and were nontoxic toward cells that represented the main models of individual organs.
PB  - Elsevier
T2  - Bioorganic & Medicinal Chemistry Letters
T1  - Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity
VL  - 112
SP  - 129928
DO  - 10.1016/j.bmcl.2024.129928
ER  - 

@article{
author = "Matošević, Ana and Bartolić, Marija and Maraković, Nikola and Zandona, Antonio and Petrić, Rajo and Opsenica, Dejan and Bosak, Anita",
year = "2024",
abstract = "Alzheimer’s disease is age-related multifactorial neurodegenerative disease manifested by gradual loss of memory, cognitive decline and changes in personality. Due to rapid and continuous growth of its prevalence, the treatment of Alzheimer’s disease calls for development of new and efficacies drugs, especially those that could be able to simultaneously act on more than one of possible targets of action. Aminoquinolines have proven to be a highly promising structural scaffold in the design of such a drug as cholinesterases and β-secretase 1 inhibitors. In this study, we synthesised twenty-two new 4-aminoquinolines with different halogen atom and its position in the terminal N-benzyl group or with a trifluoromethyl or a chlorine as C(7)-substituents on the quinoline moiety. All compounds were evaluated as multi-target-directedligands by determining their inhibition potency towards human acetylcholinesterase, butyrylcholinesterase and β-secretase 1. All of the tested derivatives were very potent inhibitors of human acetylcholinesterase and butyrylcholinesterase with inhibition constants (Ki) in the nM to low μM range. Most were estimated to be able to cross the blood–brain barrier by passive transport and were nontoxic toward cells that represented the main models of individual organs.",
publisher = "Elsevier",
journal = "Bioorganic & Medicinal Chemistry Letters",
title = "Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity",
volume = "112",
pages = "129928",
doi = "10.1016/j.bmcl.2024.129928"
}

Matošević, A., Bartolić, M., Maraković, N., Zandona, A., Petrić, R., Opsenica, D.,& Bosak, A.. (2024). Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. in Bioorganic & Medicinal Chemistry Letters
Elsevier., 112, 129928.
https://doi.org/10.1016/j.bmcl.2024.129928

Matošević A, Bartolić M, Maraković N, Zandona A, Petrić R, Opsenica D, Bosak A. Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. in Bioorganic & Medicinal Chemistry Letters. 2024;112:129928.
doi:10.1016/j.bmcl.2024.129928 .

Matošević, Ana, Bartolić, Marija, Maraković, Nikola, Zandona, Antonio, Petrić, Rajo, Opsenica, Dejan, Bosak, Anita, "Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity" in Bioorganic & Medicinal Chemistry Letters, 112 (2024):129928,
https://doi.org/10.1016/j.bmcl.2024.129928 . .

TY  - DATA
AU  - Matošević, Ana
AU  - Bartolić, Marija
AU  - Maraković, Nikola
AU  - Zandona, Antonio
AU  - Petrić, Rajo
AU  - Opsenica, Dejan
AU  - Bosak, Anita
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6618
AB  - Alzheimer’s disease is age-related multifactorial neurodegenerative disease manifested by gradual loss of memory, cognitive decline and changes in personality. Due to rapid and continuous growth of its prevalence, the treatment of Alzheimer’s disease calls for development of new and efficacies drugs, especially those that could be able to simultaneously act on more than one of possible targets of action. Aminoquinolines have proven to be a highly promising structural scaffold in the design of such a drug as cholinesterases and β-secretase 1 inhibitors. In this study, we synthesised twenty-two new 4-aminoquinolines with different halogen atom and its position in the terminal N-benzyl group or with a trifluoromethyl or a chlorine as C(7)-substituents on the quinoline moiety. All compounds were evaluated as multi-target-directedligands by determining their inhibition potency towards human acetylcholinesterase, butyrylcholinesterase and β-secretase 1. All of the tested derivatives were very potent inhibitors of human acetylcholinesterase and butyrylcholinesterase with inhibition constants (Ki) in the nM to low μM range. Most were estimated to be able to cross the blood–brain barrier by passive transport and were nontoxic toward cells that represented the main models of individual organs.
PB  - Elsevier
T2  - Bioorganic & Medicinal Chemistry Letters
T1  - Supplementary material for the article: A. Matošević, M. Bartolić, N. Maraković, A. Zandona, R. Petrić, D. Opsenica, A. Bosak; Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. Bioorganic & Medicinal Chemistry Letters 112 (2024) 129928.
VL  - 112
SP  - 129928
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6618
ER  - 

@misc{
author = "Matošević, Ana and Bartolić, Marija and Maraković, Nikola and Zandona, Antonio and Petrić, Rajo and Opsenica, Dejan and Bosak, Anita",
year = "2024",
abstract = "Alzheimer’s disease is age-related multifactorial neurodegenerative disease manifested by gradual loss of memory, cognitive decline and changes in personality. Due to rapid and continuous growth of its prevalence, the treatment of Alzheimer’s disease calls for development of new and efficacies drugs, especially those that could be able to simultaneously act on more than one of possible targets of action. Aminoquinolines have proven to be a highly promising structural scaffold in the design of such a drug as cholinesterases and β-secretase 1 inhibitors. In this study, we synthesised twenty-two new 4-aminoquinolines with different halogen atom and its position in the terminal N-benzyl group or with a trifluoromethyl or a chlorine as C(7)-substituents on the quinoline moiety. All compounds were evaluated as multi-target-directedligands by determining their inhibition potency towards human acetylcholinesterase, butyrylcholinesterase and β-secretase 1. All of the tested derivatives were very potent inhibitors of human acetylcholinesterase and butyrylcholinesterase with inhibition constants (Ki) in the nM to low μM range. Most were estimated to be able to cross the blood–brain barrier by passive transport and were nontoxic toward cells that represented the main models of individual organs.",
publisher = "Elsevier",
journal = "Bioorganic & Medicinal Chemistry Letters",
title = "Supplementary material for the article: A. Matošević, M. Bartolić, N. Maraković, A. Zandona, R. Petrić, D. Opsenica, A. Bosak; Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. Bioorganic & Medicinal Chemistry Letters 112 (2024) 129928.",
volume = "112",
pages = "129928",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6618"
}

Matošević, A., Bartolić, M., Maraković, N., Zandona, A., Petrić, R., Opsenica, D.,& Bosak, A.. (2024). Supplementary material for the article: A. Matošević, M. Bartolić, N. Maraković, A. Zandona, R. Petrić, D. Opsenica, A. Bosak; Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. Bioorganic & Medicinal Chemistry Letters 112 (2024) 129928.. in Bioorganic & Medicinal Chemistry Letters
Elsevier., 112, 129928.
https://hdl.handle.net/21.15107/rcub_cherry_6618

Matošević A, Bartolić M, Maraković N, Zandona A, Petrić R, Opsenica D, Bosak A. Supplementary material for the article: A. Matošević, M. Bartolić, N. Maraković, A. Zandona, R. Petrić, D. Opsenica, A. Bosak; Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. Bioorganic & Medicinal Chemistry Letters 112 (2024) 129928.. in Bioorganic & Medicinal Chemistry Letters. 2024;112:129928.
https://hdl.handle.net/21.15107/rcub_cherry_6618 .

Matošević, Ana, Bartolić, Marija, Maraković, Nikola, Zandona, Antonio, Petrić, Rajo, Opsenica, Dejan, Bosak, Anita, "Supplementary material for the article: A. Matošević, M. Bartolić, N. Maraković, A. Zandona, R. Petrić, D. Opsenica, A. Bosak; Synthesis and biological evaluation of novel aminoquinolines with an n-octyl linker: Impact of halogen substituents on C(7) or a terminal amino group on anticholinesterase and BACE1 activity. Bioorganic & Medicinal Chemistry Letters 112 (2024) 129928." in Bioorganic & Medicinal Chemistry Letters, 112 (2024):129928,
https://hdl.handle.net/21.15107/rcub_cherry_6618 .

TY  - CONF
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Pavlović, Marija
AU  - Šokarda Slavić, Marinela
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6612
AB  - Recent technological development has focused on addressing two major global issues: environmental protection and conservation of natural resources. High levels of dye production and use have led to significant environmental concerns due to large quantities of unmanaged colored wastewater. Textile dyes, in particular, are highly reactive and problematic when released into the environment.This study investigates the use of Aspergillus fungi biomass, a byproduct of enzyme production (xylanase, cellulase, amylase, and other hydrolytic enzymes), for removing textile dyes from wastewater. The strains Aspergillus niger, Aspergillus oryzae, Aspergillus welwitschiae, and Aspergillus tubingensis were cultivated on corn meal under optimized conditions for enzyme production. The adsorption capacity of the biomass was tested on eight textile dyes: Bezaktiv Gelb, Procion reactive yellow, Reactive yellow, Golden yellow, Procion amber, Congo red, Reactive black 5, and Remazol brilliant blue.The best results were achieved with Aspergillus oryzae biomass, particularly for Reactive black 5. Optimal adsorption conditions identified by DoE were pH 4-5 and a temperature of 25°C. Within the first hour, approximately 90% of the dye (2.16 mg/g biomass) was adsorbed, and after 24 hours, over 99% was adsorbed (2.4 mg/g biomass).The findings demonstrate that post-enzyme production biomass, typically considered waste, can effectively remove reactive dyes and other xenobiotics from wastewater. This dual benefit reduces waste and mitigates water pollution, contributing significantly to environmental protection.
PB  - Izmir Institute of Technology
C3  - V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye
T1  - From Enzyme Production to Environmental Cleanup: The Dual Role of Aspergillus Biomass
SP  - PP40
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6612
ER  - 

@conference{
author = "Margetić, Aleksandra and Ristović, Marina and Stojanović, Sanja and Pavlović, Marija and Šokarda Slavić, Marinela and Vujčić, Zoran and Dojnov, Biljana",
year = "2024",
abstract = "Recent technological development has focused on addressing two major global issues: environmental protection and conservation of natural resources. High levels of dye production and use have led to significant environmental concerns due to large quantities of unmanaged colored wastewater. Textile dyes, in particular, are highly reactive and problematic when released into the environment.This study investigates the use of Aspergillus fungi biomass, a byproduct of enzyme production (xylanase, cellulase, amylase, and other hydrolytic enzymes), for removing textile dyes from wastewater. The strains Aspergillus niger, Aspergillus oryzae, Aspergillus welwitschiae, and Aspergillus tubingensis were cultivated on corn meal under optimized conditions for enzyme production. The adsorption capacity of the biomass was tested on eight textile dyes: Bezaktiv Gelb, Procion reactive yellow, Reactive yellow, Golden yellow, Procion amber, Congo red, Reactive black 5, and Remazol brilliant blue.The best results were achieved with Aspergillus oryzae biomass, particularly for Reactive black 5. Optimal adsorption conditions identified by DoE were pH 4-5 and a temperature of 25°C. Within the first hour, approximately 90% of the dye (2.16 mg/g biomass) was adsorbed, and after 24 hours, over 99% was adsorbed (2.4 mg/g biomass).The findings demonstrate that post-enzyme production biomass, typically considered waste, can effectively remove reactive dyes and other xenobiotics from wastewater. This dual benefit reduces waste and mitigates water pollution, contributing significantly to environmental protection.",
publisher = "Izmir Institute of Technology",
journal = "V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye",
title = "From Enzyme Production to Environmental Cleanup: The Dual Role of Aspergillus Biomass",
pages = "PP40",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6612"
}

Margetić, A., Ristović, M., Stojanović, S., Pavlović, M., Šokarda Slavić, M., Vujčić, Z.,& Dojnov, B.. (2024). From Enzyme Production to Environmental Cleanup: The Dual Role of Aspergillus Biomass. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye
Izmir Institute of Technology., PP40.
https://hdl.handle.net/21.15107/rcub_cherry_6612

Margetić A, Ristović M, Stojanović S, Pavlović M, Šokarda Slavić M, Vujčić Z, Dojnov B. From Enzyme Production to Environmental Cleanup: The Dual Role of Aspergillus Biomass. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye. 2024;:PP40.
https://hdl.handle.net/21.15107/rcub_cherry_6612 .

Margetić, Aleksandra, Ristović, Marina, Stojanović, Sanja, Pavlović, Marija, Šokarda Slavić, Marinela, Vujčić, Zoran, Dojnov, Biljana, "From Enzyme Production to Environmental Cleanup: The Dual Role of Aspergillus Biomass" in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye (2024):PP40,
https://hdl.handle.net/21.15107/rcub_cherry_6612 .

TY  - CONF
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Margetić, Aleksandra
AU  - Božić, Nataša
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6611
AB  - Recently, the exploration of agricultural by-products for biotechnological applications has gained substantial interest. This study addresses the multiple potential of corn agricultural waste, focusing on its dual utility: cellulase production and enhanced polyphenols extraction.Aspergillus welwitschiae FAW1 and Aspergillus tubingensis FAT36 strains, isolated from the environment, has proven to be good producers of the cellulase enzyme complex and at the same time non-toxigenic and safe for use in food production. Due to the fact that most of the fungal enzymes are inducible, the substrates used in this solid-state fermentation were agro-waste materials richin cellulose and hemicellulose (corn silk and corn cob). The cellulase complex was produced by growing fungal strains at 28℃for 96 h.Through enzyme assisted extraction, the release of polyphenols from corn silk was enhanced. The obtained extracts, whether we used FAW1 or FAT36 strain`s cellulase enzyme complex showed an increase in total phenolic content, ranging from 876 mg/mL to 3050 mg/mL and 2944 mg/mL, respectively, which was determined by the Folin Ciocalteu method. Increasing the amount of extractedpolyphenols led to the two to three times higher antioxidant activity of the extracts, according to ABTS and FRAP methods.This enzymatic approach offers a sustainable and eco-friendly alternative to conventional extraction methods, mitigating environmental impact while enhancing product purity. These findings not only contribute to the valorization of agricultural waste, but also emphasize the potential of corn agro-wasteas a versatile resource for bioprocessing applications, connecting enzyme production, natural product extraction and added value products obtaining.
PB  - Izmir Institute of Technology
C3  - V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye
T1  - Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction
SP  - PP17
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6611
ER  - 

@conference{
author = "Stojanović, Sanja and Ristović, Marina and Margetić, Aleksandra and Božić, Nataša and Vujčić, Zoran and Dojnov, Biljana and Vujčić, Miroslava",
year = "2024",
abstract = "Recently, the exploration of agricultural by-products for biotechnological applications has gained substantial interest. This study addresses the multiple potential of corn agricultural waste, focusing on its dual utility: cellulase production and enhanced polyphenols extraction.Aspergillus welwitschiae FAW1 and Aspergillus tubingensis FAT36 strains, isolated from the environment, has proven to be good producers of the cellulase enzyme complex and at the same time non-toxigenic and safe for use in food production. Due to the fact that most of the fungal enzymes are inducible, the substrates used in this solid-state fermentation were agro-waste materials richin cellulose and hemicellulose (corn silk and corn cob). The cellulase complex was produced by growing fungal strains at 28℃for 96 h.Through enzyme assisted extraction, the release of polyphenols from corn silk was enhanced. The obtained extracts, whether we used FAW1 or FAT36 strain`s cellulase enzyme complex showed an increase in total phenolic content, ranging from 876 mg/mL to 3050 mg/mL and 2944 mg/mL, respectively, which was determined by the Folin Ciocalteu method. Increasing the amount of extractedpolyphenols led to the two to three times higher antioxidant activity of the extracts, according to ABTS and FRAP methods.This enzymatic approach offers a sustainable and eco-friendly alternative to conventional extraction methods, mitigating environmental impact while enhancing product purity. These findings not only contribute to the valorization of agricultural waste, but also emphasize the potential of corn agro-wasteas a versatile resource for bioprocessing applications, connecting enzyme production, natural product extraction and added value products obtaining.",
publisher = "Izmir Institute of Technology",
journal = "V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye",
title = "Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction",
pages = "PP17",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6611"
}

Stojanović, S., Ristović, M., Margetić, A., Božić, N., Vujčić, Z., Dojnov, B.,& Vujčić, M.. (2024). Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye
Izmir Institute of Technology., PP17.
https://hdl.handle.net/21.15107/rcub_cherry_6611

Stojanović S, Ristović M, Margetić A, Božić N, Vujčić Z, Dojnov B, Vujčić M. Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction. in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye. 2024;:PP17.
https://hdl.handle.net/21.15107/rcub_cherry_6611 .

Stojanović, Sanja, Ristović, Marina, Margetić, Aleksandra, Božić, Nataša, Vujčić, Zoran, Dojnov, Biljana, Vujčić, Miroslava, "Corn agro-waste valorization: A dual approach towards cellulase production and increased polyphenol extraction" in V International Enzyme & Bioprocess Days, Abstract Book, August 27-29, 2024; İzmir, Türkiye (2024):PP17,
https://hdl.handle.net/21.15107/rcub_cherry_6611 .

TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Nikolić, Milan
AU  - Stojanović, Srđan Đ.
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6607
AB  - In this in silico study, it was investigated whether phycobilins
(phycocyanobilin, phycoerythrobilin and phycourobilin) could be inhibitors of
the activity of the main proteins of the SARS-CoV-2 virus. All chromophores
exhibited a binding energy value of ≥–37 kJ mol-1 for PLpro-WT, PLpro-
-C111S, helicase-ANP binding site, Nsp3-macrodomain, Nsp3-MES site and
Nsp10/14-N7-Mtase. Phycocyanobilin showed the highest binding energy of
–44.77 kJ mol-1 against the target protein PLpro-C111S. It was found that,
apart from the hydrogen bonds and hydrophobic interactions, phycobilins also
form electrostatic interactions with the SARS-CoV-2 proteins. The network of
non-covalent interactions was found to be important for the stability of the
examined virus proteins. All phycobilins have good pharmacokinetic and drug-
-likeness properties. This study’s results suggest that the screened phycobilins
could serve as promising drugs for the treatment of COVID-19 with further
rigorous validation studies.
PB  - Belgrade : Serbian Chemical Society
T2  - Jornal of Serbian Chemical Society
T1  - In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19
VL  - 89
IS  - 7-8
SP  - 997
EP  - 1009
DO  - 10.2298/JSC240326052J
ER  - 

@article{
author = "Jovanović, Vesna B. and Nikolić, Milan and Stojanović, Srđan Đ.",
year = "2024",
abstract = "In this in silico study, it was investigated whether phycobilins
(phycocyanobilin, phycoerythrobilin and phycourobilin) could be inhibitors of
the activity of the main proteins of the SARS-CoV-2 virus. All chromophores
exhibited a binding energy value of ≥–37 kJ mol-1 for PLpro-WT, PLpro-
-C111S, helicase-ANP binding site, Nsp3-macrodomain, Nsp3-MES site and
Nsp10/14-N7-Mtase. Phycocyanobilin showed the highest binding energy of
–44.77 kJ mol-1 against the target protein PLpro-C111S. It was found that,
apart from the hydrogen bonds and hydrophobic interactions, phycobilins also
form electrostatic interactions with the SARS-CoV-2 proteins. The network of
non-covalent interactions was found to be important for the stability of the
examined virus proteins. All phycobilins have good pharmacokinetic and drug-
-likeness properties. This study’s results suggest that the screened phycobilins
could serve as promising drugs for the treatment of COVID-19 with further
rigorous validation studies.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Jornal of Serbian Chemical Society",
title = "In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19",
volume = "89",
number = "7-8",
pages = "997-1009",
doi = "10.2298/JSC240326052J"
}

Jovanović, V. B., Nikolić, M.,& Stojanović, S. Đ.. (2024). In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19. in Jornal of Serbian Chemical Society
Belgrade : Serbian Chemical Society., 89(7-8), 997-1009.
https://doi.org/10.2298/JSC240326052J

Jovanović VB, Nikolić M, Stojanović SĐ. In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19. in Jornal of Serbian Chemical Society. 2024;89(7-8):997-1009.
doi:10.2298/JSC240326052J .

Jovanović, Vesna B., Nikolić, Milan, Stojanović, Srđan Đ., "In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19" in Jornal of Serbian Chemical Society, 89, no. 7-8 (2024):997-1009,
https://doi.org/10.2298/JSC240326052J . .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Jovanović, Zorana
AU  - Cvijetić, Ilija
AU  - Šunderić, Miloš
AU  - Veličković, Luka
AU  - Katrlík, Jaroslav
AU  - Holazová, Alena
AU  - Nikolić, Milan
AU  - Minić, Simeon
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6524
AB  - Blue C-phycocyanin (C-PC), the major Spirulina protein with innumerable health-promoting
benefits, is an attractive colourant and food supplement. A crucial obstacle to its more extensive
use is its relatively low stability. This study aimed to screen various food-derived ligands for their
ability to bind and stabilise C-PC, utilising spectroscopic techniques and molecular docking. Among twelve examined ligands, the protein fluorescence quenching revealed that only quercetin, coenzyme Q10 and resveratrol had a moderate affinity to C-PC (Ka of 2.2 to 3.7 × 105 M–1). Docking revealed these three ligands bind more strongly to the C-PC hexamer than the trimer, with the binding sites located at the interface of two (αβ)3 trimers. UV/VIS absorption spectroscopy demonstrated the changes in the C-PC absorption spectra in a complex with quercetin and resveratrol compared to the spectra of free protein and ligands. Selected ligands did not affect the secondary structure content, but they induced changes in the tertiary protein structure in the CD study. A fluorescence-based thermal stability assay demonstrated quercetin and coenzyme Q10 increased the C-PC melting point by nearly 5 ◦C. Our study identified food-derived ligands that interact with C-PC and improve its thermal stability, indicating their potential as stabilising agents for C-PC in the food industry.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina
DO  - 10.3390/ijms25010229
ER  - 

@article{
author = "Gligorijević, Nikola and Jovanović, Zorana and Cvijetić, Ilija and Šunderić, Miloš and Veličković, Luka and Katrlík, Jaroslav and Holazová, Alena and Nikolić, Milan and Minić, Simeon",
year = "2024",
abstract = "Blue C-phycocyanin (C-PC), the major Spirulina protein with innumerable health-promoting
benefits, is an attractive colourant and food supplement. A crucial obstacle to its more extensive
use is its relatively low stability. This study aimed to screen various food-derived ligands for their
ability to bind and stabilise C-PC, utilising spectroscopic techniques and molecular docking. Among twelve examined ligands, the protein fluorescence quenching revealed that only quercetin, coenzyme Q10 and resveratrol had a moderate affinity to C-PC (Ka of 2.2 to 3.7 × 105 M–1). Docking revealed these three ligands bind more strongly to the C-PC hexamer than the trimer, with the binding sites located at the interface of two (αβ)3 trimers. UV/VIS absorption spectroscopy demonstrated the changes in the C-PC absorption spectra in a complex with quercetin and resveratrol compared to the spectra of free protein and ligands. Selected ligands did not affect the secondary structure content, but they induced changes in the tertiary protein structure in the CD study. A fluorescence-based thermal stability assay demonstrated quercetin and coenzyme Q10 increased the C-PC melting point by nearly 5 ◦C. Our study identified food-derived ligands that interact with C-PC and improve its thermal stability, indicating their potential as stabilising agents for C-PC in the food industry.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina",
doi = "10.3390/ijms25010229"
}

Gligorijević, N., Jovanović, Z., Cvijetić, I., Šunderić, M., Veličković, L., Katrlík, J., Holazová, A., Nikolić, M.,& Minić, S.. (2024). Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina. in International Journal of Molecular Sciences
MDPI..
https://doi.org/10.3390/ijms25010229

Gligorijević N, Jovanović Z, Cvijetić I, Šunderić M, Veličković L, Katrlík J, Holazová A, Nikolić M, Minić S. Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina. in International Journal of Molecular Sciences. 2024;.
doi:10.3390/ijms25010229 .

Gligorijević, Nikola, Jovanović, Zorana, Cvijetić, Ilija, Šunderić, Miloš, Veličković, Luka, Katrlík, Jaroslav, Holazová, Alena, Nikolić, Milan, Minić, Simeon, "Investigation of the Potential of Selected Food-Derived Antioxidants to Bind and Stabilise the Bioactive Blue Protein C-Phycocyanin from Cyanobacteria Spirulina" in International Journal of Molecular Sciences (2024),
https://doi.org/10.3390/ijms25010229 . .

TY  - JOUR
AU  - Jednak Berić, Tanja
AU  - Vrvić, Miroslav
AU  - Lješević, Marija
AU  - Avdalović, Jelena
AU  - Ilić, Mila
AU  - Crnković, Dragan
AU  - Jovančićević, Branimir
AU  - Miletić, Srđan
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6604
AB  - Environmental contextMitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents.RationaleBioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies.MethodologyIn this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction.ResultsIn our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased.DiscussionThe sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.
PB  - CSIRO Publishing
T2  - Environmental Chemistry
T1  - Supplementary material for the article: T. Jednak Berić, M. M. Vrvić, M. Lješević, J. Avdalović, M. Ilić, D. Crnković, B. Jovančićević, S. Miletić; Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. Environmental Chemistry 21 (2024) EN23111. https://doi.org/10.1071/EN23111
VL  - 21
IS  - 5
SP  - EN23111
DO  - 10.1071/EN23111
ER  - 

@article{
author = "Jednak Berić, Tanja and Vrvić, Miroslav and Lješević, Marija and Avdalović, Jelena and Ilić, Mila and Crnković, Dragan and Jovančićević, Branimir and Miletić, Srđan",
year = "2024",
abstract = "Environmental contextMitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents.RationaleBioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies.MethodologyIn this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction.ResultsIn our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased.DiscussionThe sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.",
publisher = "CSIRO Publishing",
journal = "Environmental Chemistry",
title = "Supplementary material for the article: T. Jednak Berić, M. M. Vrvić, M. Lješević, J. Avdalović, M. Ilić, D. Crnković, B. Jovančićević, S. Miletić; Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. Environmental Chemistry 21 (2024) EN23111. https://doi.org/10.1071/EN23111",
volume = "21",
number = "5",
pages = "EN23111",
doi = "10.1071/EN23111"
}

Jednak Berić, T., Vrvić, M., Lješević, M., Avdalović, J., Ilić, M., Crnković, D., Jovančićević, B.,& Miletić, S.. (2024). Supplementary material for the article: T. Jednak Berić, M. M. Vrvić, M. Lješević, J. Avdalović, M. Ilić, D. Crnković, B. Jovančićević, S. Miletić; Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. Environmental Chemistry 21 (2024) EN23111. https://doi.org/10.1071/EN23111. in Environmental Chemistry
CSIRO Publishing., 21(5), EN23111.
https://doi.org/10.1071/EN23111

Jednak Berić T, Vrvić M, Lješević M, Avdalović J, Ilić M, Crnković D, Jovančićević B, Miletić S. Supplementary material for the article: T. Jednak Berić, M. M. Vrvić, M. Lješević, J. Avdalović, M. Ilić, D. Crnković, B. Jovančićević, S. Miletić; Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. Environmental Chemistry 21 (2024) EN23111. https://doi.org/10.1071/EN23111. in Environmental Chemistry. 2024;21(5):EN23111.
doi:10.1071/EN23111 .

Jednak Berić, Tanja, Vrvić, Miroslav, Lješević, Marija, Avdalović, Jelena, Ilić, Mila, Crnković, Dragan, Jovančićević, Branimir, Miletić, Srđan, "Supplementary material for the article: T. Jednak Berić, M. M. Vrvić, M. Lješević, J. Avdalović, M. Ilić, D. Crnković, B. Jovančićević, S. Miletić; Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. Environmental Chemistry 21 (2024) EN23111. https://doi.org/10.1071/EN23111" in Environmental Chemistry, 21, no. 5 (2024):EN23111,
https://doi.org/10.1071/EN23111 . .

TY  - JOUR
AU  - Jednak Berić, Tanja
AU  - Vrvić, Miroslav
AU  - Lješević, Marija
AU  - Avdalović, Jelena
AU  - Ilić, Mila
AU  - Crnković, Dragan
AU  - Jovančićević, Branimir
AU  - Miletić, Srđan
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6579
AB  - Environmental contextMitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents.RationaleBioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies.MethodologyIn this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction.ResultsIn our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased.DiscussionThe sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.
PB  - CSIRO Publishing
T2  - Environmental Chemistry
T1  - Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage
VL  - 21
IS  - 5
SP  - EN23111
DO  - 10.1071/EN23111
ER  - 

@article{
author = "Jednak Berić, Tanja and Vrvić, Miroslav and Lješević, Marija and Avdalović, Jelena and Ilić, Mila and Crnković, Dragan and Jovančićević, Branimir and Miletić, Srđan",
year = "2024",
abstract = "Environmental contextMitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents.RationaleBioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies.MethodologyIn this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction.ResultsIn our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased.DiscussionThe sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.",
publisher = "CSIRO Publishing",
journal = "Environmental Chemistry",
title = "Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage",
volume = "21",
number = "5",
pages = "EN23111",
doi = "10.1071/EN23111"
}

Jednak Berić, T., Vrvić, M., Lješević, M., Avdalović, J., Ilić, M., Crnković, D., Jovančićević, B.,& Miletić, S.. (2024). Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. in Environmental Chemistry
CSIRO Publishing., 21(5), EN23111.
https://doi.org/10.1071/EN23111

Jednak Berić T, Vrvić M, Lješević M, Avdalović J, Ilić M, Crnković D, Jovančićević B, Miletić S. Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage. in Environmental Chemistry. 2024;21(5):EN23111.
doi:10.1071/EN23111 .

Jednak Berić, Tanja, Vrvić, Miroslav, Lješević, Marija, Avdalović, Jelena, Ilić, Mila, Crnković, Dragan, Jovančićević, Branimir, Miletić, Srđan, "Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage" in Environmental Chemistry, 21, no. 5 (2024):EN23111,
https://doi.org/10.1071/EN23111 . .

TY  - JOUR
AU  - Vranić, Sofija
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Todosijević, Marina
AU  - Pantelić, Dejan
AU  - Pavlović, Danica
AU  - Ivanović, Stefan
AU  - Vasović, Marija
AU  - Ćurčić, Srećko
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6598
AB  - Ground beetles possess a pair of pygidial glands that produce and release secretions that play an important role in defense against predators. The morphology of these glands and the chemical composition of their products were studied in four species of the tribe Sphodrini: Calathus (Calathus) fuscipes (Goeze, 1777), C. (Neocalathus) cinctus Motschulsky, 1850, C. (N.) melanocephalus (Linnaeus, 1758) and Laemostenus (Antisphodrus) elongatus (Dejean, 1828). The morphological analyzes of the glands of the four taxa mentioned were carried out for the first time using bright-field and nonlinear microscopy. All morphological structures were precisely measured and photographed. The pygidial gland secretions of C. (C.) fuscipes and L. (A.) elongatus were analyzed for the first time using gas chromatography-mass spectrometry. A total of 30 compounds were detected from the extracts of pygidial gland secretions of the four Sphodrini species studied. The simplest chemical mixture was found in L. (A.) elongatus, while the most complex secretion was that of C. (C.) fuscipes. 1-Undecanol, which we were able to detect in all taxa examined here, and dodecyl butyrate, which was detected in the three Calathus species, have never before been detected in the secretions of ground beetles.
PB  - Elsevier
T2  - Journal of Insect Physiology
T1  - The morphology of the pygidial glands and the chemical composition of their secretions of four sphodrine ground beetle species (Carabidae: Platyninae)
VL  - 158
SP  - 104685
DO  - 10.1016/j.jinsphys.2024.104685
ER  - 

@article{
author = "Vranić, Sofija and Vujisić, Ljubodrag and Vesović, Nikola and Todosijević, Marina and Pantelić, Dejan and Pavlović, Danica and Ivanović, Stefan and Vasović, Marija and Ćurčić, Srećko",
year = "2024",
abstract = "Ground beetles possess a pair of pygidial glands that produce and release secretions that play an important role in defense against predators. The morphology of these glands and the chemical composition of their products were studied in four species of the tribe Sphodrini: Calathus (Calathus) fuscipes (Goeze, 1777), C. (Neocalathus) cinctus Motschulsky, 1850, C. (N.) melanocephalus (Linnaeus, 1758) and Laemostenus (Antisphodrus) elongatus (Dejean, 1828). The morphological analyzes of the glands of the four taxa mentioned were carried out for the first time using bright-field and nonlinear microscopy. All morphological structures were precisely measured and photographed. The pygidial gland secretions of C. (C.) fuscipes and L. (A.) elongatus were analyzed for the first time using gas chromatography-mass spectrometry. A total of 30 compounds were detected from the extracts of pygidial gland secretions of the four Sphodrini species studied. The simplest chemical mixture was found in L. (A.) elongatus, while the most complex secretion was that of C. (C.) fuscipes. 1-Undecanol, which we were able to detect in all taxa examined here, and dodecyl butyrate, which was detected in the three Calathus species, have never before been detected in the secretions of ground beetles.",
publisher = "Elsevier",
journal = "Journal of Insect Physiology",
title = "The morphology of the pygidial glands and the chemical composition of their secretions of four sphodrine ground beetle species (Carabidae: Platyninae)",
volume = "158",
pages = "104685",
doi = "10.1016/j.jinsphys.2024.104685"
}

Vranić, S., Vujisić, L., Vesović, N., Todosijević, M., Pantelić, D., Pavlović, D., Ivanović, S., Vasović, M.,& Ćurčić, S.. (2024). The morphology of the pygidial glands and the chemical composition of their secretions of four sphodrine ground beetle species (Carabidae: Platyninae). in Journal of Insect Physiology
Elsevier., 158, 104685.
https://doi.org/10.1016/j.jinsphys.2024.104685

Vranić S, Vujisić L, Vesović N, Todosijević M, Pantelić D, Pavlović D, Ivanović S, Vasović M, Ćurčić S. The morphology of the pygidial glands and the chemical composition of their secretions of four sphodrine ground beetle species (Carabidae: Platyninae). in Journal of Insect Physiology. 2024;158:104685.
doi:10.1016/j.jinsphys.2024.104685 .

Vranić, Sofija, Vujisić, Ljubodrag, Vesović, Nikola, Todosijević, Marina, Pantelić, Dejan, Pavlović, Danica, Ivanović, Stefan, Vasović, Marija, Ćurčić, Srećko, "The morphology of the pygidial glands and the chemical composition of their secretions of four sphodrine ground beetle species (Carabidae: Platyninae)" in Journal of Insect Physiology, 158 (2024):104685,
https://doi.org/10.1016/j.jinsphys.2024.104685 . .

TY  - JOUR
AU  - Minić, Simeon
AU  - Veličković, Luka
AU  - Annighöfer, Burkhard
AU  - Thureau, Aurélien
AU  - Gligorijević, Nikola
AU  - Jovanović, Zorana
AU  - Brûlet, Annie
AU  - Combet, Sophie
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6595
AB  - The red macroalgae Porphyra, commonly known as Nori, is widely used as food around the world due to its high nutrient content, including the significant abundance of colored phycobiliproteins (PBPs). Among these, R-phycocyanin (R-PC) stands out for its vibrant purple color and numerous bioactive properties, making it a valuable protein for the food industry. However, R-PC's limited thermal stability necessitates alternative processing methods to preserve its color and bioactive properties. Our study aimed to investigate the in-situ stability of oligomeric R-PC under high pressure (HP) conditions (up to 4000 bar) using a combination of absorption, fluorescence, and small-angle X-ray scattering (SAXS) techniques. The unfolding of R-PC is a multiphase process. Initially, low pressure induces conformational changes in the R-PC oligomeric form (trimers). As pressure increases above 1600 bar, these trimers dissociate into monomers, and at pressures above 3000 bar, the subunits begin to unfold. When returned to atmospheric pressure, R-PC partially refolds, retaining 50% of its original color absorbance. In contrast, heat treatment causes irreversible and detrimental effects on R-PC color, highlighting the advantages of HP treatment in preserving both the color and bioactive properties of R-PC compared to heat treatment.
PB  - Wiley
T2  - Protein Science
T1  - Probing the structural stability of R-phycocyanin under pressure
VL  - 33
IS  - 9
SP  - e5145
DO  - 10.1002/pro.5145
ER  - 

@article{
author = "Minić, Simeon and Veličković, Luka and Annighöfer, Burkhard and Thureau, Aurélien and Gligorijević, Nikola and Jovanović, Zorana and Brûlet, Annie and Combet, Sophie",
year = "2024",
abstract = "The red macroalgae Porphyra, commonly known as Nori, is widely used as food around the world due to its high nutrient content, including the significant abundance of colored phycobiliproteins (PBPs). Among these, R-phycocyanin (R-PC) stands out for its vibrant purple color and numerous bioactive properties, making it a valuable protein for the food industry. However, R-PC's limited thermal stability necessitates alternative processing methods to preserve its color and bioactive properties. Our study aimed to investigate the in-situ stability of oligomeric R-PC under high pressure (HP) conditions (up to 4000 bar) using a combination of absorption, fluorescence, and small-angle X-ray scattering (SAXS) techniques. The unfolding of R-PC is a multiphase process. Initially, low pressure induces conformational changes in the R-PC oligomeric form (trimers). As pressure increases above 1600 bar, these trimers dissociate into monomers, and at pressures above 3000 bar, the subunits begin to unfold. When returned to atmospheric pressure, R-PC partially refolds, retaining 50% of its original color absorbance. In contrast, heat treatment causes irreversible and detrimental effects on R-PC color, highlighting the advantages of HP treatment in preserving both the color and bioactive properties of R-PC compared to heat treatment.",
publisher = "Wiley",
journal = "Protein Science",
title = "Probing the structural stability of R-phycocyanin under pressure",
volume = "33",
number = "9",
pages = "e5145",
doi = "10.1002/pro.5145"
}

Minić, S., Veličković, L., Annighöfer, B., Thureau, A., Gligorijević, N., Jovanović, Z., Brûlet, A.,& Combet, S.. (2024). Probing the structural stability of R-phycocyanin under pressure. in Protein Science
Wiley., 33(9), e5145.
https://doi.org/10.1002/pro.5145

Minić S, Veličković L, Annighöfer B, Thureau A, Gligorijević N, Jovanović Z, Brûlet A, Combet S. Probing the structural stability of R-phycocyanin under pressure. in Protein Science. 2024;33(9):e5145.
doi:10.1002/pro.5145 .

Minić, Simeon, Veličković, Luka, Annighöfer, Burkhard, Thureau, Aurélien, Gligorijević, Nikola, Jovanović, Zorana, Brûlet, Annie, Combet, Sophie, "Probing the structural stability of R-phycocyanin under pressure" in Protein Science, 33, no. 9 (2024):e5145,
https://doi.org/10.1002/pro.5145 . .

TY  - JOUR
AU  - Lukić, Marija
AU  - Avdalović, Jelena
AU  - Gojgić-Cvijović, Gordana
AU  - Žerađanin, Aleksandra
AU  - Mrazovac Kurilić, Sanja
AU  - Ilić, Mila
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
AU  - Beškoski, Vladimir
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6581
AB  - The aim of this paper is to provide insight into research and activities of in situ remediation to remove petroleum hydrocarbon pollutants from a contaminated aquifer’s sediment, located near two radial collector wells of a water supply system. It was decided that the most appropriate method for remediation of this aquifer’s sediment is in situ bioremediation because it is clean, efficient and sustainable technology. Before the start of the bioremediation process, it was necessary to isolate and cultivate the microorganisms present at the contamination site, so they could be later applied in the bioremediation process. The samples before and after the bioremediation were studied using both GC and GC × GC–MS to determine how the concentrations of contaminants changed over time. Additionally, in this paper, a spatiotemporal representation of the change in hydrocarbon content by depth within the zone of the highest contamination over time is shown. After 12 months of bioremediation, the hydrocarbon content in the samples decreased by 82.0%, and based on GCxGC-MS analysis, the order of degradation of various hydrocarbon groups was as follows: steranes (99.6%), isoprenoids (98.4%), benzene derivatives (98.4%), alkanes (97.2%), and terpenes (49.3%). The exponential decay model showed the greatest decomposition rate of hydrocarbons occurred at depths of 8–10 m, with an average decay constant of 0.227, independent of the initial concentration of hydrocarbons. To the best of our knowledge, to date, the described approach has not been applied to an aquifer (the simultaneous treatment of groundwater and its associated sediment layers).
PB  - Springer Link
T2  - Clean Technologies and Environmental Policy
T1  - Industrial-scale bioremediation of a hydrocarbon-contaminated aquifer’s sediment at the location of a heating plant, Belgrade, Serbia
VL  - 26
IS  - 6
SP  - 1785
EP  - 1798
DO  - 10.1007/s10098-023-02724-8
ER  - 

@article{
author = "Lukić, Marija and Avdalović, Jelena and Gojgić-Cvijović, Gordana and Žerađanin, Aleksandra and Mrazovac Kurilić, Sanja and Ilić, Mila and Miletić, Srđan and Vrvić, Miroslav and Beškoski, Vladimir",
year = "2024",
abstract = "The aim of this paper is to provide insight into research and activities of in situ remediation to remove petroleum hydrocarbon pollutants from a contaminated aquifer’s sediment, located near two radial collector wells of a water supply system. It was decided that the most appropriate method for remediation of this aquifer’s sediment is in situ bioremediation because it is clean, efficient and sustainable technology. Before the start of the bioremediation process, it was necessary to isolate and cultivate the microorganisms present at the contamination site, so they could be later applied in the bioremediation process. The samples before and after the bioremediation were studied using both GC and GC × GC–MS to determine how the concentrations of contaminants changed over time. Additionally, in this paper, a spatiotemporal representation of the change in hydrocarbon content by depth within the zone of the highest contamination over time is shown. After 12 months of bioremediation, the hydrocarbon content in the samples decreased by 82.0%, and based on GCxGC-MS analysis, the order of degradation of various hydrocarbon groups was as follows: steranes (99.6%), isoprenoids (98.4%), benzene derivatives (98.4%), alkanes (97.2%), and terpenes (49.3%). The exponential decay model showed the greatest decomposition rate of hydrocarbons occurred at depths of 8–10 m, with an average decay constant of 0.227, independent of the initial concentration of hydrocarbons. To the best of our knowledge, to date, the described approach has not been applied to an aquifer (the simultaneous treatment of groundwater and its associated sediment layers).",
publisher = "Springer Link",
journal = "Clean Technologies and Environmental Policy",
title = "Industrial-scale bioremediation of a hydrocarbon-contaminated aquifer’s sediment at the location of a heating plant, Belgrade, Serbia",
volume = "26",
number = "6",
pages = "1785-1798",
doi = "10.1007/s10098-023-02724-8"
}

Lukić, M., Avdalović, J., Gojgić-Cvijović, G., Žerađanin, A., Mrazovac Kurilić, S., Ilić, M., Miletić, S., Vrvić, M.,& Beškoski, V.. (2024). Industrial-scale bioremediation of a hydrocarbon-contaminated aquifer’s sediment at the location of a heating plant, Belgrade, Serbia. in Clean Technologies and Environmental Policy
Springer Link., 26(6), 1785-1798.
https://doi.org/10.1007/s10098-023-02724-8

Lukić M, Avdalović J, Gojgić-Cvijović G, Žerađanin A, Mrazovac Kurilić S, Ilić M, Miletić S, Vrvić M, Beškoski V. Industrial-scale bioremediation of a hydrocarbon-contaminated aquifer’s sediment at the location of a heating plant, Belgrade, Serbia. in Clean Technologies and Environmental Policy. 2024;26(6):1785-1798.
doi:10.1007/s10098-023-02724-8 .

Lukić, Marija, Avdalović, Jelena, Gojgić-Cvijović, Gordana, Žerađanin, Aleksandra, Mrazovac Kurilić, Sanja, Ilić, Mila, Miletić, Srđan, Vrvić, Miroslav, Beškoski, Vladimir, "Industrial-scale bioremediation of a hydrocarbon-contaminated aquifer’s sediment at the location of a heating plant, Belgrade, Serbia" in Clean Technologies and Environmental Policy, 26, no. 6 (2024):1785-1798,
https://doi.org/10.1007/s10098-023-02724-8 . .

TY  - GEN
AU  - Jednak Berić, Tanja
AU  - Vrvić, Miroslav
AU  - Lješević, Marija
AU  - Avdalović, Jelena
AU  - Ilić, Mila
AU  - Crnković, Dragan
AU  - Jovančićević, Branimir
AU  - Miletić, Srđan
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6580
AB  - Environmental context: Mitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents. Rationale: Bioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies. Methodology: In this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction. Results: In our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased. Discussion: The sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.
PB  - CSIRO Publishing
T2  - Environmental Chemistry
T1  - Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage (pre-publication version)
VL  - 21
IS  - 5
SP  - EN23111
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6580
ER  - 

@misc{
author = "Jednak Berić, Tanja and Vrvić, Miroslav and Lješević, Marija and Avdalović, Jelena and Ilić, Mila and Crnković, Dragan and Jovančićević, Branimir and Miletić, Srđan",
year = "2024",
abstract = "Environmental context: Mitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents. Rationale: Bioremediation harnesses microorganisms’ diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies. Methodology: In this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma–optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction. Results: In our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased. Discussion: The sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.",
publisher = "CSIRO Publishing",
journal = "Environmental Chemistry",
title = "Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage (pre-publication version)",
volume = "21",
number = "5",
pages = "EN23111",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6580"
}

Jednak Berić, T., Vrvić, M., Lješević, M., Avdalović, J., Ilić, M., Crnković, D., Jovančićević, B.,& Miletić, S.. (2024). Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage (pre-publication version). in Environmental Chemistry
CSIRO Publishing., 21(5), EN23111.
https://hdl.handle.net/21.15107/rcub_cherry_6580

Jednak Berić T, Vrvić M, Lješević M, Avdalović J, Ilić M, Crnković D, Jovančićević B, Miletić S. Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage (pre-publication version). in Environmental Chemistry. 2024;21(5):EN23111.
https://hdl.handle.net/21.15107/rcub_cherry_6580 .

Jednak Berić, Tanja, Vrvić, Miroslav, Lješević, Marija, Avdalović, Jelena, Ilić, Mila, Crnković, Dragan, Jovančićević, Branimir, Miletić, Srđan, "Testing of the bioremediation on model substrates for complex refinery contaminants arising from accidental or deliberate facility damage (pre-publication version)" in Environmental Chemistry, 21, no. 5 (2024):EN23111,
https://hdl.handle.net/21.15107/rcub_cherry_6580 .

TY  - JOUR
AU  - Trmčić, Milena
AU  - Vulović, Bojan
AU  - Zlatar, Matija
AU  - Saičić, Radomir
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6536
AB  - Hemiacetals of cyclopropanone can be isolated and stocked, contrary to their highly reactive parent ketone.  However, they are not readily converted to cyclopropanone, which limits their use as its synthetic equivalents.  2,2,2-trifluoroethoxy hemiacetals are expected to be better cyclopropanone surrogates, however, they have  never been prepared, so far. We show that oxyallyl cations with a heteroatom in the -position can be  intercepted with 2,2,2-trifluoroethanol, with formation of cyclopropanone trifluoroethoxy hemiacetals stable  enough to be isolated, purified and characterized. These species can serve as synthetic equivalents of  cyclopropanone under mild conditions.
PB  - ARKAT USA, Inc.
T2  - ARKIVOC - Online Journal of Organic Chemistry
T1  - Preparation of cyclopropanone 2,2,2-trifluoroethoxy hemiacetals via oxyallyl cation
VL  - 2024
IS  - 2
DO  - 10.24820/ark.5550190.p012.123
ER  - 

@article{
author = "Trmčić, Milena and Vulović, Bojan and Zlatar, Matija and Saičić, Radomir",
year = "2024",
abstract = "Hemiacetals of cyclopropanone can be isolated and stocked, contrary to their highly reactive parent ketone.  However, they are not readily converted to cyclopropanone, which limits their use as its synthetic equivalents.  2,2,2-trifluoroethoxy hemiacetals are expected to be better cyclopropanone surrogates, however, they have  never been prepared, so far. We show that oxyallyl cations with a heteroatom in the -position can be  intercepted with 2,2,2-trifluoroethanol, with formation of cyclopropanone trifluoroethoxy hemiacetals stable  enough to be isolated, purified and characterized. These species can serve as synthetic equivalents of  cyclopropanone under mild conditions.",
publisher = "ARKAT USA, Inc.",
journal = "ARKIVOC - Online Journal of Organic Chemistry",
title = "Preparation of cyclopropanone 2,2,2-trifluoroethoxy hemiacetals via oxyallyl cation",
volume = "2024",
number = "2",
doi = "10.24820/ark.5550190.p012.123"
}

Trmčić, M., Vulović, B., Zlatar, M.,& Saičić, R.. (2024). Preparation of cyclopropanone 2,2,2-trifluoroethoxy hemiacetals via oxyallyl cation. in ARKIVOC - Online Journal of Organic Chemistry
ARKAT USA, Inc.., 2024(2).
https://doi.org/10.24820/ark.5550190.p012.123

Trmčić M, Vulović B, Zlatar M, Saičić R. Preparation of cyclopropanone 2,2,2-trifluoroethoxy hemiacetals via oxyallyl cation. in ARKIVOC - Online Journal of Organic Chemistry. 2024;2024(2).
doi:10.24820/ark.5550190.p012.123 .

Trmčić, Milena, Vulović, Bojan, Zlatar, Matija, Saičić, Radomir, "Preparation of cyclopropanone 2,2,2-trifluoroethoxy hemiacetals via oxyallyl cation" in ARKIVOC - Online Journal of Organic Chemistry, 2024, no. 2 (2024),
https://doi.org/10.24820/ark.5550190.p012.123 . .

TY  - JOUR
AU  - Jakšić, Jovana
AU  - Milinković, Evgenija
AU  - Cvetanović, Katarina
AU  - Tokić Vujošević, Zorana
AU  - Jovanov, Vladislav
AU  - Mitrović, Aleksandra D.
AU  - Maslak, Veselin
PY  - 2024
UR  - https://pubs.rsc.org/en/content/articlelanding/2024/cp/d3cp04322c
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6422
AB  - In this study,we conducted a comprehensive investigation of newly synthesized fullerene derivatives developed for potential application in perovskite solar cells (PSCs). We explored three novel dihydrofuran-fused C60 fullerene derivatives (13, 14, and 15) that we respecifically designed to enhance solubility and interaction with the substrate, fluorine-dopedtinoxide (FTO). A comparative analysis was performed, with reference to the widely used phenyl-C61-butyric acid methyl ester (PCBM) and compound 12, from which 13, 14, and 15 are derived, to assess the impact of sugar units on materials properties.The synthesized compounds demonstrated significant solubility in common organic solvents, a critical factor in their potential application in wet-processed PSCs. Our investigation included electrochemical property analysis, thin film deposition, surface characterization, and electrochemical impedance spectroscopy (EIS). EIS measurements unveiled key in sights into charge transfer properties at the electrode/electrolyte interface, making the compounds attractive candidates for electron transport layers (ETLs) in PSCs.
T2  - Physical Chemistry Chemical Physics
T1  - Exploring fullerene derivatives for optoelectronic applications: synthesis and characterization study
VL  - 26
IS  - 1
SP  - 517
EP  - 523
DO  - 10.1039/D3CP04322C
ER  - 

@article{
author = "Jakšić, Jovana and Milinković, Evgenija and Cvetanović, Katarina and Tokić Vujošević, Zorana and Jovanov, Vladislav and Mitrović, Aleksandra D. and Maslak, Veselin",
year = "2024",
abstract = "In this study,we conducted a comprehensive investigation of newly synthesized fullerene derivatives developed for potential application in perovskite solar cells (PSCs). We explored three novel dihydrofuran-fused C60 fullerene derivatives (13, 14, and 15) that we respecifically designed to enhance solubility and interaction with the substrate, fluorine-dopedtinoxide (FTO). A comparative analysis was performed, with reference to the widely used phenyl-C61-butyric acid methyl ester (PCBM) and compound 12, from which 13, 14, and 15 are derived, to assess the impact of sugar units on materials properties.The synthesized compounds demonstrated significant solubility in common organic solvents, a critical factor in their potential application in wet-processed PSCs. Our investigation included electrochemical property analysis, thin film deposition, surface characterization, and electrochemical impedance spectroscopy (EIS). EIS measurements unveiled key in sights into charge transfer properties at the electrode/electrolyte interface, making the compounds attractive candidates for electron transport layers (ETLs) in PSCs.",
journal = "Physical Chemistry Chemical Physics",
title = "Exploring fullerene derivatives for optoelectronic applications: synthesis and characterization study",
volume = "26",
number = "1",
pages = "517-523",
doi = "10.1039/D3CP04322C"
}

Jakšić, J., Milinković, E., Cvetanović, K., Tokić Vujošević, Z., Jovanov, V., Mitrović, A. D.,& Maslak, V.. (2024). Exploring fullerene derivatives for optoelectronic applications: synthesis and characterization study. in Physical Chemistry Chemical Physics, 26(1), 517-523.
https://doi.org/10.1039/D3CP04322C

Jakšić J, Milinković E, Cvetanović K, Tokić Vujošević Z, Jovanov V, Mitrović AD, Maslak V. Exploring fullerene derivatives for optoelectronic applications: synthesis and characterization study. in Physical Chemistry Chemical Physics. 2024;26(1):517-523.
doi:10.1039/D3CP04322C .

Jakšić, Jovana, Milinković, Evgenija, Cvetanović, Katarina, Tokić Vujošević, Zorana, Jovanov, Vladislav, Mitrović, Aleksandra D., Maslak, Veselin, "Exploring fullerene derivatives for optoelectronic applications: synthesis and characterization study" in Physical Chemistry Chemical Physics, 26, no. 1 (2024):517-523,
https://doi.org/10.1039/D3CP04322C . .

TY  - CONF
AU  - Vizi, Aleksa
AU  - Radović, Nebojša R.
AU  - Nikolić, Željka, M.
AU  - Lekić, Stefan D.
AU  - Roglić, Goran
AU  - Stojanović, Ksenija
AU  - Tešević, Vele
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6547
AB  - Dimethyl fumarate (DMF) serves as the active ingredient in medications designed to treat
relapsing forms of multiple sclerosis, yet there is a notable absence of specific monographs
for this compound in available pharmacopeia’s. Nevertheless, various instrumental
chromatographic techniques are employed to determine the concentration of DMF across
diverse matrices.
This study endeavours to highlight the viability of merging structural instrumental
analytical methods with a simple classical volumetric approach utilizing a cost-effective, non-
toxic, and natural indicator - the ethanol extract of Curcuma longa powder. The primary aim
is to accurately determine DMF assay in commercial samples without utilizing certified
reference materials (CRM).
PB  - University of Belgrade, Technical Faculty in Bor, Serbia
C3  - 31st International conference Ecological Truth & Environmental Research - EcoTER'24, Sokobanja, Serbia, 18-21 June 2024
T1  - Sustainable Solutions in Analytical Chemistry: Combining of Instrumental Techniques and Environmental-Friendly Natural Indicators for Classical Volumetry
T1  - Održiva rešenja u analitičkoj hemiji: Kombinacija instrumentalnih tehnika i ekološki prihvatljivih prirodnih indikatora za klasičnu volumetriju
SP  - 751
EP  - 752
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6547
ER  - 

@conference{
author = "Vizi, Aleksa and Radović, Nebojša R. and Nikolić, Željka, M. and Lekić, Stefan D. and Roglić, Goran and Stojanović, Ksenija and Tešević, Vele",
year = "2024",
abstract = "Dimethyl fumarate (DMF) serves as the active ingredient in medications designed to treat
relapsing forms of multiple sclerosis, yet there is a notable absence of specific monographs
for this compound in available pharmacopeia’s. Nevertheless, various instrumental
chromatographic techniques are employed to determine the concentration of DMF across
diverse matrices.
This study endeavours to highlight the viability of merging structural instrumental
analytical methods with a simple classical volumetric approach utilizing a cost-effective, non-
toxic, and natural indicator - the ethanol extract of Curcuma longa powder. The primary aim
is to accurately determine DMF assay in commercial samples without utilizing certified
reference materials (CRM).",
publisher = "University of Belgrade, Technical Faculty in Bor, Serbia",
journal = "31st International conference Ecological Truth & Environmental Research - EcoTER'24, Sokobanja, Serbia, 18-21 June 2024",
title = "Sustainable Solutions in Analytical Chemistry: Combining of Instrumental Techniques and Environmental-Friendly Natural Indicators for Classical Volumetry, Održiva rešenja u analitičkoj hemiji: Kombinacija instrumentalnih tehnika i ekološki prihvatljivih prirodnih indikatora za klasičnu volumetriju",
pages = "751-752",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6547"
}

Vizi, A., Radović, N. R., Nikolić, Ž. M., Lekić, S. D., Roglić, G., Stojanović, K.,& Tešević, V.. (2024). Sustainable Solutions in Analytical Chemistry: Combining of Instrumental Techniques and Environmental-Friendly Natural Indicators for Classical Volumetry. in 31st International conference Ecological Truth & Environmental Research - EcoTER'24, Sokobanja, Serbia, 18-21 June 2024
University of Belgrade, Technical Faculty in Bor, Serbia., 751-752.
https://hdl.handle.net/21.15107/rcub_cherry_6547

Vizi A, Radović NR, Nikolić ŽM, Lekić SD, Roglić G, Stojanović K, Tešević V. Sustainable Solutions in Analytical Chemistry: Combining of Instrumental Techniques and Environmental-Friendly Natural Indicators for Classical Volumetry. in 31st International conference Ecological Truth & Environmental Research - EcoTER'24, Sokobanja, Serbia, 18-21 June 2024. 2024;:751-752.
https://hdl.handle.net/21.15107/rcub_cherry_6547 .

Vizi, Aleksa, Radović, Nebojša R., Nikolić, Željka, M., Lekić, Stefan D., Roglić, Goran, Stojanović, Ksenija, Tešević, Vele, "Sustainable Solutions in Analytical Chemistry: Combining of Instrumental Techniques and Environmental-Friendly Natural Indicators for Classical Volumetry" in 31st International conference Ecological Truth & Environmental Research - EcoTER'24, Sokobanja, Serbia, 18-21 June 2024 (2024):751-752,
https://hdl.handle.net/21.15107/rcub_cherry_6547 .

TY  - JOUR
AU  - Selaković, Snežana
AU  - Rodić, Marko V.
AU  - Novaković, Irena T.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Milčić, Miloš
AU  - Medaković, Vesna
AU  - Dimiza, Filitsa
AU  - Psomas, George
AU  - Anđelković, Katarina K.
AU  - Šumar-Ristović, Maja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6537
AB  - Copper(II) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(II) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf–thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.
PB  - Royal Society of Chemistry
T2  - Dalton Transactions
T2  - Dalton TransactionsDalton Trans.
T1  - Cu(ii) complexes with a salicylaldehyde derivative and a-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach
VL  - 53
IS  - 6
SP  - 2770
EP  - 2788
DO  - 10.1039/D3DT03862A
ER  - 

@article{
author = "Selaković, Snežana and Rodić, Marko V. and Novaković, Irena T. and Matić, Ivana Z. and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Milčić, Miloš and Medaković, Vesna and Dimiza, Filitsa and Psomas, George and Anđelković, Katarina K. and Šumar-Ristović, Maja",
year = "2024",
abstract = "Copper(II) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(II) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf–thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.",
publisher = "Royal Society of Chemistry",
journal = "Dalton Transactions, Dalton TransactionsDalton Trans.",
title = "Cu(ii) complexes with a salicylaldehyde derivative and a-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach",
volume = "53",
number = "6",
pages = "2770-2788",
doi = "10.1039/D3DT03862A"
}

Selaković, S., Rodić, M. V., Novaković, I. T., Matić, I. Z., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Milčić, M., Medaković, V., Dimiza, F., Psomas, G., Anđelković, K. K.,& Šumar-Ristović, M.. (2024). Cu(ii) complexes with a salicylaldehyde derivative and a-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions
Royal Society of Chemistry., 53(6), 2770-2788.
https://doi.org/10.1039/D3DT03862A

Selaković S, Rodić MV, Novaković IT, Matić IZ, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Milčić M, Medaković V, Dimiza F, Psomas G, Anđelković KK, Šumar-Ristović M. Cu(ii) complexes with a salicylaldehyde derivative and a-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions. 2024;53(6):2770-2788.
doi:10.1039/D3DT03862A .

Selaković, Snežana, Rodić, Marko V., Novaković, Irena T., Matić, Ivana Z., Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Milčić, Miloš, Medaković, Vesna, Dimiza, Filitsa, Psomas, George, Anđelković, Katarina K., Šumar-Ristović, Maja, "Cu(ii) complexes with a salicylaldehyde derivative and a-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach" in Dalton Transactions, 53, no. 6 (2024):2770-2788,
https://doi.org/10.1039/D3DT03862A . .

TY  - CONF
AU  - Marković, Olivera S.
AU  - Mrđinac, Jelena Ž.
AU  - Pepić, Sara V.
AU  - Verbić, Tatjana Ž.
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6530
AB  - The solubility profile of a drug typically consists of two independent curves that intersect 
at a specific point called pHmax. At pHmax, both salt and base coexist as solid phases. While 
two solids coexist, pH and solubility in the heterogeneous system remain constant 
(according to the Gibbs Phase Rule). Recent studies, however, have shown that pH and 
solubility might change during the salt-to-free-base transformation. This pH range, 
containing two solids, is termed the pHmax – pHmin range. pHmax refers to the pH value at 
the maximum solubility of a eutectic mixture, whereas pHmin represents the pH value at the 
minimum solubility of the eutectic mixture. The objective of this study was to investigate 
the influence of suspension concentration (the ratio of drug mass to suspension volume) on 
the pHmax – pHmin range during solubility determination using the pH-Ramp shake-flask
method. Nortriptyline hydrochloride (Nor, monoprotic base) and atorvastatin calcium (At, 
monoprotic acid) were employed as model compounds. Results indicate that the pHmax –
pHmin range increases as suspension concentration rises (for 20.10 mg NorHCl/mL: 
pHmax=3.55, ΔpH=0; for 59.82 mg NorHCl/mL: pHmax – pHmin= 3.56 – 4.52, ΔpH=0.96). 
Understanding the details of the pHmax – pHmin range could significantly impact product 
formulation optimization in drug research
AB  - Cilj ovog rada bio je ispitivanje uticaja koncentracije suspenzije na širinu pHmax – pHmin
opsega tokom određivanja rastvorljivosti pH-Ramp shake-flask metodom. Kao model 
jedinjenja izabrani su nortriptilin-hidrohlorid i atorvastatin-kalcijum. Rezultati su pokazali 
da sa porastom koncentracije suspenzije dolazi do širenja opsega pHmax – pHmin, a ovakva 
proučavanja mogu značajno doprineti optimizaciji formulacije u procesu dizajna i razvoja 
lekova.
PB  - Belgrade : Serbian Chemical Society
C3  - 60th Meeting of the Serbian Chemical Society, Niš, Serbia, 8-9 June 2024
T1  - The influence of suspension concentration on the pHmax – pHmin range during the solubility determination
T1  - Ispitivanje uticaja koncentracije suspenzije na širinu pHmax – pHmin opsega tokom određivanja rastvorljivosti
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6530
ER  - 

@conference{
author = "Marković, Olivera S. and Mrđinac, Jelena Ž. and Pepić, Sara V. and Verbić, Tatjana Ž.",
year = "2024",
abstract = "The solubility profile of a drug typically consists of two independent curves that intersect 
at a specific point called pHmax. At pHmax, both salt and base coexist as solid phases. While 
two solids coexist, pH and solubility in the heterogeneous system remain constant 
(according to the Gibbs Phase Rule). Recent studies, however, have shown that pH and 
solubility might change during the salt-to-free-base transformation. This pH range, 
containing two solids, is termed the pHmax – pHmin range. pHmax refers to the pH value at 
the maximum solubility of a eutectic mixture, whereas pHmin represents the pH value at the 
minimum solubility of the eutectic mixture. The objective of this study was to investigate 
the influence of suspension concentration (the ratio of drug mass to suspension volume) on 
the pHmax – pHmin range during solubility determination using the pH-Ramp shake-flask
method. Nortriptyline hydrochloride (Nor, monoprotic base) and atorvastatin calcium (At, 
monoprotic acid) were employed as model compounds. Results indicate that the pHmax –
pHmin range increases as suspension concentration rises (for 20.10 mg NorHCl/mL: 
pHmax=3.55, ΔpH=0; for 59.82 mg NorHCl/mL: pHmax – pHmin= 3.56 – 4.52, ΔpH=0.96). 
Understanding the details of the pHmax – pHmin range could significantly impact product 
formulation optimization in drug research, Cilj ovog rada bio je ispitivanje uticaja koncentracije suspenzije na širinu pHmax – pHmin
opsega tokom određivanja rastvorljivosti pH-Ramp shake-flask metodom. Kao model 
jedinjenja izabrani su nortriptilin-hidrohlorid i atorvastatin-kalcijum. Rezultati su pokazali 
da sa porastom koncentracije suspenzije dolazi do širenja opsega pHmax – pHmin, a ovakva 
proučavanja mogu značajno doprineti optimizaciji formulacije u procesu dizajna i razvoja 
lekova.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "60th Meeting of the Serbian Chemical Society, Niš, Serbia, 8-9 June 2024",
title = "The influence of suspension concentration on the pHmax – pHmin range during the solubility determination, Ispitivanje uticaja koncentracije suspenzije na širinu pHmax – pHmin opsega tokom određivanja rastvorljivosti",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6530"
}

Marković, O. S., Mrđinac, J. Ž., Pepić, S. V.,& Verbić, T. Ž.. (2024). The influence of suspension concentration on the pHmax – pHmin range during the solubility determination. in 60th Meeting of the Serbian Chemical Society, Niš, Serbia, 8-9 June 2024
Belgrade : Serbian Chemical Society., 47-47.
https://hdl.handle.net/21.15107/rcub_cherry_6530

Marković OS, Mrđinac JŽ, Pepić SV, Verbić TŽ. The influence of suspension concentration on the pHmax – pHmin range during the solubility determination. in 60th Meeting of the Serbian Chemical Society, Niš, Serbia, 8-9 June 2024. 2024;:47-47.
https://hdl.handle.net/21.15107/rcub_cherry_6530 .

Marković, Olivera S., Mrđinac, Jelena Ž., Pepić, Sara V., Verbić, Tatjana Ž., "The influence of suspension concentration on the pHmax – pHmin range during the solubility determination" in 60th Meeting of the Serbian Chemical Society, Niš, Serbia, 8-9 June 2024 (2024):47-47,
https://hdl.handle.net/21.15107/rcub_cherry_6530 .

TY  - JOUR
AU  - Dorontić, Slađana
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Sredojević, Dušan
AU  - Ciasca, Gabriele
AU  - Di Santo, Riccardo
AU  - Mead, James L.
AU  - Budimir, Milica
AU  - Bajuk-Bogdanović, Danica
AU  - Mojsin, Marija
AU  - Pejić, Jelena
AU  - Stevanović, Milena
AU  - Jovanović, Svetlana
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6523
AB  - The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, 
simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum 
dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative 
structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocom patibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs 
structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, 
and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to 
the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich 
GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. 
The high coefficient of determination (R-squared (R2
) = 0.99) and very low limit of detection (3.02 μmol L-1) 
reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully 
detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the 
proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the 
death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an 
air medium can be potentially applied for glyphosate detection.
PB  - Elsevier
T2  - Journal of Environmental Chemical Engineering
T1  - High-performing structural optimization of graphene quantum dots as  glyphosate herbicide photoluminescent probes: real case studies and  mechanism insights
VL  - 12
SP  - 113193
DO  - 10.1016/j.jece.2024.113193
ER  - 

@article{
author = "Dorontić, Slađana and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Sredojević, Dušan and Ciasca, Gabriele and Di Santo, Riccardo and Mead, James L. and Budimir, Milica and Bajuk-Bogdanović, Danica and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, 
simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum 
dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative 
structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocom patibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs 
structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, 
and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to 
the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich 
GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. 
The high coefficient of determination (R-squared (R2
) = 0.99) and very low limit of detection (3.02 μmol L-1) 
reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully 
detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the 
proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the 
death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an 
air medium can be potentially applied for glyphosate detection.",
publisher = "Elsevier",
journal = "Journal of Environmental Chemical Engineering",
title = "High-performing structural optimization of graphene quantum dots as  glyphosate herbicide photoluminescent probes: real case studies and  mechanism insights",
volume = "12",
pages = "113193",
doi = "10.1016/j.jece.2024.113193"
}

Dorontić, S., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Sredojević, D., Ciasca, G., Di Santo, R., Mead, J. L., Budimir, M., Bajuk-Bogdanović, D., Mojsin, M., Pejić, J., Stevanović, M.,& Jovanović, S.. (2024). High-performing structural optimization of graphene quantum dots as  glyphosate herbicide photoluminescent probes: real case studies and  mechanism insights. in Journal of Environmental Chemical Engineering
Elsevier., 12, 113193.
https://doi.org/10.1016/j.jece.2024.113193

Dorontić S, Bonasera A, Scopelliti M, Marković O, Verbić T, Sredojević D, Ciasca G, Di Santo R, Mead JL, Budimir M, Bajuk-Bogdanović D, Mojsin M, Pejić J, Stevanović M, Jovanović S. High-performing structural optimization of graphene quantum dots as  glyphosate herbicide photoluminescent probes: real case studies and  mechanism insights. in Journal of Environmental Chemical Engineering. 2024;12:113193.
doi:10.1016/j.jece.2024.113193 .

Dorontić, Slađana, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Sredojević, Dušan, Ciasca, Gabriele, Di Santo, Riccardo, Mead, James L., Budimir, Milica, Bajuk-Bogdanović, Danica, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena, Jovanović, Svetlana, "High-performing structural optimization of graphene quantum dots as  glyphosate herbicide photoluminescent probes: real case studies and  mechanism insights" in Journal of Environmental Chemical Engineering, 12 (2024):113193,
https://doi.org/10.1016/j.jece.2024.113193 . .

TY  - JOUR
AU  - Milenković, Mila
AU  - Ciasca, Gabriele
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Todorović Marković, Biljana
AU  - Jovanović, Svetlana
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6414
AB  - The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L-cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.
PB  - Elsevier
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects
VL  - 250
SP  - 112818
DO  - 10.1016/j.jphotobiol.2023.112818
ER  - 

@article{
author = "Milenković, Mila and Ciasca, Gabriele and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Todorović Marković, Biljana and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L-cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.",
publisher = "Elsevier",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects",
volume = "250",
pages = "112818",
doi = "10.1016/j.jphotobiol.2023.112818"
}

Milenković, M., Ciasca, G., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Todorović Marković, B.,& Jovanović, S.. (2024). Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects. in Journal of Photochemistry and Photobiology B: Biology
Elsevier., 250, 112818.
https://doi.org/10.1016/j.jphotobiol.2023.112818

Milenković M, Ciasca G, Bonasera A, Scopelliti M, Marković O, Verbić T, Todorović Marković B, Jovanović S. Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects. in Journal of Photochemistry and Photobiology B: Biology. 2024;250:112818.
doi:10.1016/j.jphotobiol.2023.112818 .

Milenković, Mila, Ciasca, Gabriele, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Todorović Marković, Biljana, Jovanović, Svetlana, "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum dots and their antibacterial effects" in Journal of Photochemistry and Photobiology B: Biology, 250 (2024):112818,
https://doi.org/10.1016/j.jphotobiol.2023.112818 . .

TY  - JOUR
AU  - Milenković, Mila
AU  - Ciasca, Gabriele
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Todorović Marković, Biljana
AU  - Jovanović, Svetlana
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6303
AB  - The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give 
in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, 
smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the 
antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. 
Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic 
resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of 
modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in 
the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol 
groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 
4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed 
that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs 
combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 
sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against 
S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.
PB  - Elsevier
T2  - Journal of Photochemistry & Photobiology, B: Biology
T1  - Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects
VL  - 250
SP  - 112818
DO  - 10.1016/j.jphotobiol.2023.112818
ER  - 

@article{
author = "Milenković, Mila and Ciasca, Gabriele and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Todorović Marković, Biljana and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give 
in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, 
smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the 
antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. 
Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic 
resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of 
modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in 
the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol 
groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 
4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed 
that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs 
combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 
sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against 
S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.",
publisher = "Elsevier",
journal = "Journal of Photochemistry & Photobiology, B: Biology",
title = "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects",
volume = "250",
pages = "112818",
doi = "10.1016/j.jphotobiol.2023.112818"
}

Milenković, M., Ciasca, G., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Todorović Marković, B.,& Jovanović, S.. (2024). Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology
Elsevier., 250, 112818.
https://doi.org/10.1016/j.jphotobiol.2023.112818

Milenković M, Ciasca G, Bonasera A, Scopelliti M, Marković O, Verbić T, Todorović Marković B, Jovanović S. Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology. 2024;250:112818.
doi:10.1016/j.jphotobiol.2023.112818 .

Milenković, Mila, Ciasca, Gabriele, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Todorović Marković, Biljana, Jovanović, Svetlana, "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects" in Journal of Photochemistry & Photobiology, B: Biology, 250 (2024):112818,
https://doi.org/10.1016/j.jphotobiol.2023.112818 . .

TY  - DATA
AU  - Šokarda Slavić, Marinela
AU  - Margetić, Aleksandra
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Mišić, Milan
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5836
AB  - Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.
T2  - Fuel
T1  - Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363
VL  - 338
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5836
ER  - 

@misc{
author = "Šokarda Slavić, Marinela and Margetić, Aleksandra and Dojnov, Biljana and Vujčić, Miroslava and Mišić, Milan and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "Bioethanol is one of the main bio-based molecules produced mainly from sugar cane, molasses and corn. Its environmental advantages allow it to be considered as safe and the cleanest fuel alternative. Starch is a widespread renewable carbohydrate conventionally used for bioethanol production via energy demanding liquefaction and saccharification processes. Raw starch hydrolysis using enzymes capable of degrading it below the gelatinization temperature significantly simplifies the process and reduces the cost of starch processing. In this study, an innovative modified simultaneous saccharification and fermentation process is proposed for the production of bioethanol from highly concentrated raw corn starch (30 % w/v). A two-step synergistic hydrolysis and fermentation was carried out in a single bioreactor vessel. To ensure high process efficiency, factors influencing the hydrolysis of concentrated raw corn starch by raw starch degrading α-amylase from Bacillus paralicheniformis ATCC 9945a (BliAmy) and commercial glucoamylase were investigated. Box–Behnken experimental design was used to predict the effects of different ratios of added enzymes, glucoamylase addition time, incubation time, and pH on hydrolysis yield. Optimal conditions for the highest yield of hydrolysis of raw corn starch (90 %) were obtained after 8 h using 5.0 IU BliAmy per mg of starch and 0.5 % (v/v) glucoamylase at pH 4.5 and 60 °C. Obtained glucose was further fermented with Saccharomyces cerevisiae at 30 °C in the same vessel for bioethanol production. Bioethanol concentration at 129.2 g/L, with productivity of 2.94 g/L/h and ethanol yield (YP/S) at 0.50 g EtOH/g total sugar, equivalent to 87.8 % theoretical yield, was obtained by modified simultaneous saccharification and fermentation. This work enriches the information of bioethanol production and offers a novel strategy for raw starch hydrolysis under industrial conditions.",
journal = "Fuel",
title = "Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363",
volume = "338",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5836"
}

Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363. in Fuel, 338.
https://hdl.handle.net/21.15107/rcub_cherry_5836

Šokarda Slavić M, Margetić A, Dojnov B, Vujčić M, Mišić M, Božić N, Vujčić Z. Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363. in Fuel. 2023;338.
https://hdl.handle.net/21.15107/rcub_cherry_5836 .

Šokarda Slavić, Marinela, Margetić, Aleksandra, Dojnov, Biljana, Vujčić, Miroslava, Mišić, Milan, Božić, Nataša, Vujčić, Zoran, "Supplementary material for: Šokarda Slavić, M., Margetić, A., Dojnov, B., Vujčić, M., Mišić, M., Božić, N.,& Vujčić, Z.. (2023). Modified simultaneous saccharification and fermentation for the production of bioethanol from highly concentrated raw corn starch. in Fuel, 338, 127363. https://doi.org/10.1016/j.fuel.2022.127363" in Fuel, 338 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5836 .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6388
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society
Serbian Chemical Society..
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society (2023),
https://doi.org/10.2298/JSC221116011S . .

TY  - JOUR
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5980
AB  - Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.
PB  - Informa UK Limited
T2  - Journal of Apicultural Research
T1  - In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed
VL  - 62
IS  - 5
SP  - 1007
EP  - 1016
DO  - 10.1080/00218839.2022.2080950
ER  - 

@article{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2023",
abstract = "Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.",
publisher = "Informa UK Limited",
journal = "Journal of Apicultural Research",
title = "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed",
volume = "62",
number = "5",
pages = "1007-1016",
doi = "10.1080/00218839.2022.2080950"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2023). In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research
Informa UK Limited., 62(5), 1007-1016.
https://doi.org/10.1080/00218839.2022.2080950

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research. 2023;62(5):1007-1016.
doi:10.1080/00218839.2022.2080950 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed" in Journal of Apicultural Research, 62, no. 5 (2023):1007-1016,
https://doi.org/10.1080/00218839.2022.2080950 . .