TY  - JOUR
AU  - Stanković, Vesna
AU  - Đurđić, Slađana Z.
AU  - Ognjanović, Miloš
AU  - Antić, Bratislav
AU  - Kalcher, Kurt
AU  - Mutić, Jelena
AU  - Stanković, Dalibor
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3891
AB  - Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.
PB  - Elsevier
T2  - Journal of Electroanalytical Chemistry
T1  - Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor
VL  - 860
SP  - 113928
DO  - 10.1016/j.jelechem.2020.113928
ER  - 

@article{
author = "Stanković, Vesna and Đurđić, Slađana Z. and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena and Stanković, Dalibor",
year = "2020",
abstract = "Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor",
volume = "860",
pages = "113928",
doi = "10.1016/j.jelechem.2020.113928"
}

Stanković, V., Đurđić, S. Z., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J.,& Stanković, D.. (2020). Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry
Elsevier., 860, 113928.
https://doi.org/10.1016/j.jelechem.2020.113928

Stanković V, Đurđić SZ, Ognjanović M, Antić B, Kalcher K, Mutić J, Stanković D. Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry. 2020;860:113928.
doi:10.1016/j.jelechem.2020.113928 .

Stanković, Vesna, Đurđić, Slađana Z., Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena, Stanković, Dalibor, "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor" in Journal of Electroanalytical Chemistry, 860 (2020):113928,
https://doi.org/10.1016/j.jelechem.2020.113928 . .

TY  - DATA
AU  - Stanković, Vesna
AU  - Đurđić, Slađana Z.
AU  - Ognjanović, Miloš
AU  - Antić, Bratislav
AU  - Kalcher, Kurt
AU  - Mutić, Jelena
AU  - Stanković, Dalibor
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3892
PB  - Elsevier
T2  - Journal of Electroanalytical Chemistry
T1  - Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3892
ER  - 

@misc{
author = "Stanković, Vesna and Đurđić, Slađana Z. and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena and Stanković, Dalibor",
year = "2020",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3892"
}

Stanković, V., Đurđić, S. Z., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J.,& Stanković, D.. (2020). Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928. in Journal of Electroanalytical Chemistry
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_3892

Stanković V, Đurđić SZ, Ognjanović M, Antić B, Kalcher K, Mutić J, Stanković D. Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928. in Journal of Electroanalytical Chemistry. 2020;.
https://hdl.handle.net/21.15107/rcub_cherry_3892 .

Stanković, Vesna, Đurđić, Slađana Z., Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena, Stanković, Dalibor, "Supplementary data for the article: Stanković, V.; Đurđić, S.; Ognjanović, M.; Antić, B.; Kalcher, K.; Mutić, J.; Stanković, D. M. Anti-Human Albumin Monoclonal Antibody Immobilized on EDC-NHS Functionalized Carboxylic Graphene/AuNPs Composite as Promising Electrochemical HSA Immunosensor. Journal of Electroanalytical Chemistry 2020, 860. https://doi.org/10.1016/j.jelechem.2020.113928" in Journal of Electroanalytical Chemistry (2020),
https://hdl.handle.net/21.15107/rcub_cherry_3892 .