TY  - DATA
AU  - Khulal, Urmila
AU  - Ghnimi, Sami
AU  - Stevanović, Nikola R.
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4747
PB  - Elsevier
T2  - LWT
T1  - Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.
VL  - 152
SP  - 112250
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4747
ER  - 

@misc{
author = "Khulal, Urmila and Ghnimi, Sami and Stevanović, Nikola R. and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
publisher = "Elsevier",
journal = "LWT",
title = "Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.",
volume = "152",
pages = "112250",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4747"
}

Khulal, U., Ghnimi, S., Stevanović, N. R., Rajković, A.,& Ćirković-Veličković, T.. (2021). Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.. in LWT
Elsevier., 152, 112250.
https://hdl.handle.net/21.15107/rcub_cherry_4747

Khulal U, Ghnimi S, Stevanović NR, Rajković A, Ćirković-Veličković T. Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250.. in LWT. 2021;152:112250.
https://hdl.handle.net/21.15107/rcub_cherry_4747 .

Khulal, Urmila, Ghnimi, Sami, Stevanović, Nikola R., Rajković, Andreja, Ćirković-Veličković, Tanja, "Supplementary data for the article: Khulal, U.; Ghnimi, S.; Stevanovic, N.; Rajkovic, A.; Cirkovic Velickovic, T. Aggregability and Digestibility Study of Fruit Juice Fortified Camel Milk Powder Proteins. LWT 2021, 152, 112250. https://doi.org/10.1016/j.lwt.2021.112250." in LWT, 152 (2021):112250,
https://hdl.handle.net/21.15107/rcub_cherry_4747 .

TY  - JOUR
AU  - Khulal, Urmila
AU  - Ghnimi, Sami
AU  - Stevanović, Nikola R.
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4746
AB  - In this work, we observed the effect of grape juice (% concentrated juice/% concentrated camel milk: GJ20/80, GJ50/50) and pomegranate juice (PJ20/80, PJ40/60) fortification on camel milk (CM) protein solubility and digestibility. Proteins were dissolved in sodium phosphate buffer to 50 mg/ml and defatted prior Bradford assay of protein concentration, then analyzed by Size Exclusion-Ultra High-Performance Liquid chromatography (SE-UHPLC). The CM protein aggregation and their stability were further monitored at different pH 2.0, 4.0, and 7.5 via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Freeze dried CM (FDCM) was the reference sample and our results showed that GJ50/50 and PJ40/60 with the highest fruit juice ratio had the lowest protein content in the supernatant, hence the decreased solubility. SE-UHPLC of supernatants showed a slight decrease in retention times of 11 kDa and 62 kDa proteins for GJ50/50 and PJ40/60 suggesting a possibility of adduct formation due to fortification leading to higher molecular weight. The simulated static in vitro gastrointestinal digestion of samples revealed that most soluble proteins were readily digested by pepsin, trypsin and chymotrypsin enzymes leading to small peptides. However, the SDS PAGE of pellets showed the partial resistance of casein and α-lactalbumin against peptic digestion.
PB  - Elsevier
T2  - LWT
T1  - Aggregability and digestibility study of fruit juice fortified camel milk powder proteins
VL  - 152
SP  - 112250
DO  - 10.1016/j.lwt.2021.112250
ER  - 

@article{
author = "Khulal, Urmila and Ghnimi, Sami and Stevanović, Nikola R. and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "In this work, we observed the effect of grape juice (% concentrated juice/% concentrated camel milk: GJ20/80, GJ50/50) and pomegranate juice (PJ20/80, PJ40/60) fortification on camel milk (CM) protein solubility and digestibility. Proteins were dissolved in sodium phosphate buffer to 50 mg/ml and defatted prior Bradford assay of protein concentration, then analyzed by Size Exclusion-Ultra High-Performance Liquid chromatography (SE-UHPLC). The CM protein aggregation and their stability were further monitored at different pH 2.0, 4.0, and 7.5 via sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Freeze dried CM (FDCM) was the reference sample and our results showed that GJ50/50 and PJ40/60 with the highest fruit juice ratio had the lowest protein content in the supernatant, hence the decreased solubility. SE-UHPLC of supernatants showed a slight decrease in retention times of 11 kDa and 62 kDa proteins for GJ50/50 and PJ40/60 suggesting a possibility of adduct formation due to fortification leading to higher molecular weight. The simulated static in vitro gastrointestinal digestion of samples revealed that most soluble proteins were readily digested by pepsin, trypsin and chymotrypsin enzymes leading to small peptides. However, the SDS PAGE of pellets showed the partial resistance of casein and α-lactalbumin against peptic digestion.",
publisher = "Elsevier",
journal = "LWT",
title = "Aggregability and digestibility study of fruit juice fortified camel milk powder proteins",
volume = "152",
pages = "112250",
doi = "10.1016/j.lwt.2021.112250"
}

Khulal, U., Ghnimi, S., Stevanović, N. R., Rajković, A.,& Ćirković-Veličković, T.. (2021). Aggregability and digestibility study of fruit juice fortified camel milk powder proteins. in LWT
Elsevier., 152, 112250.
https://doi.org/10.1016/j.lwt.2021.112250

Khulal U, Ghnimi S, Stevanović NR, Rajković A, Ćirković-Veličković T. Aggregability and digestibility study of fruit juice fortified camel milk powder proteins. in LWT. 2021;152:112250.
doi:10.1016/j.lwt.2021.112250 .

Khulal, Urmila, Ghnimi, Sami, Stevanović, Nikola R., Rajković, Andreja, Ćirković-Veličković, Tanja, "Aggregability and digestibility study of fruit juice fortified camel milk powder proteins" in LWT, 152 (2021):112250,
https://doi.org/10.1016/j.lwt.2021.112250 . .