TY  - JOUR
AU  - Koračak, Ljiljana
AU  - Lupšić, Ema
AU  - Terzić-Jovanović, Nataša
AU  - Jovanović, Mirna
AU  - Novakovic, Miroslav
AU  - Nedialkov, Paraskev
AU  - Trendafilova, Antoaneta
AU  - Zlatović, Mario
AU  - Pešić, Milica
AU  - Opsenica, Igor
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6245
AB  - The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.
PB  - Royal Society of Chemistry
T2  - New Journal of Chemistry
T2  - New Journal of ChemistryNew J. Chem.
T1  - Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells
VL  - 47
IS  - 14
SP  - 6844
EP  - 6855
DO  - 10.1039/D3NJ00427A
ER  - 

@article{
author = "Koračak, Ljiljana and Lupšić, Ema and Terzić-Jovanović, Nataša and Jovanović, Mirna and Novakovic, Miroslav and Nedialkov, Paraskev and Trendafilova, Antoaneta and Zlatović, Mario and Pešić, Milica and Opsenica, Igor",
year = "2023",
abstract = "The synthesis of 17 hybrid molecules, consisting of artesunate, a derivative of naturally occurring artemisinin, and synthetic 4-aryl-2-aminopyrimidines, is described. New compounds were designed to improve the parent compounds' cytotoxic properties, activity, and selectivity. The synthesized hybrid molecules (15a–f with ethylenediamine linker and 16a–k with piperazine linker), as well as their precursors – pyrimidine derivatives (13a–f and 14a–k), artemisinin, and artesunate, were tested on sensitive and multidrug-resistant (MDR) human non-small cell lung carcinoma (NSCLC) cells. All hybrid compounds with piperazine linker 16a–k were selective toward NSCLC cells and displayed IC50 values below 5 μM. Although they showed similar anticancer potency as artesunate, their selectivity against cancer cells was considerably improved. Importantly, 16h–k hybrid compounds were able to evade MDR phenotype, inhibit P-glycoprotein (P-gp) activity, and increase the sensitivity of MDR NSCLC cells to doxorubicin (DOX). The inhibition of P-gp activity induced by 16h–j was stronger than the one obtained with artesunate. Among these four hybrid compounds, 16k was the most potent anticancer agent with similar IC50 values of around 1.5 μM (for comparison – over 3.1 μM for artesunate) in sensitive and MDR NSCLC cells.",
publisher = "Royal Society of Chemistry",
journal = "New Journal of Chemistry, New Journal of ChemistryNew J. Chem.",
title = "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells",
volume = "47",
number = "14",
pages = "6844-6855",
doi = "10.1039/D3NJ00427A"
}

Koračak, L., Lupšić, E., Terzić-Jovanović, N., Jovanović, M., Novakovic, M., Nedialkov, P., Trendafilova, A., Zlatović, M., Pešić, M.,& Opsenica, I.. (2023). Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry
Royal Society of Chemistry., 47(14), 6844-6855.
https://doi.org/10.1039/D3NJ00427A

Koračak L, Lupšić E, Terzić-Jovanović N, Jovanović M, Novakovic M, Nedialkov P, Trendafilova A, Zlatović M, Pešić M, Opsenica I. Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells. in New Journal of Chemistry. 2023;47(14):6844-6855.
doi:10.1039/D3NJ00427A .

Koračak, Ljiljana, Lupšić, Ema, Terzić-Jovanović, Nataša, Jovanović, Mirna, Novakovic, Miroslav, Nedialkov, Paraskev, Trendafilova, Antoaneta, Zlatović, Mario, Pešić, Milica, Opsenica, Igor, "Novel artesunate–pyrimidine-based hybrids with anticancer potential against multidrug-resistant cancer cells" in New Journal of Chemistry, 47, no. 14 (2023):6844-6855,
https://doi.org/10.1039/D3NJ00427A . .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Pop, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana Z.
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5970
AB  - Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation (IL-1β, TNF-α, and IFN-γ) in the duodenum. Moreover, micrographs of the duodenum were in line with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used 16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting this toxic environmental pollutant.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats
VL  - 24
IS  - 3
SP  - 2845
DO  - 10.3390/ijms24032845
ER  - 

@article{
author = "Brdarić, Emilija and Pop, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana Z. and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation (IL-1β, TNF-α, and IFN-γ) in the duodenum. Moreover, micrographs of the duodenum were in line with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used 16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting this toxic environmental pollutant.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats",
volume = "24",
number = "3",
pages = "2845",
doi = "10.3390/ijms24032845"
}

Brdarić, E., Pop, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S. Z., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences
MDPI., 24(3), 2845.
https://doi.org/10.3390/ijms24032845

Brdarić E, Pop D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić SZ, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences. 2023;24(3):2845.
doi:10.3390/ijms24032845 .

Brdarić, Emilija, Pop, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana Z., Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats" in International Journal of Molecular Sciences, 24, no. 3 (2023):2845,
https://doi.org/10.3390/ijms24032845 . .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Perić, Marija
AU  - Nikolić, Biljana
AU  - Mandić, Boris
AU  - Cvetković, Stefana
AU  - Bogdanović, Milica
AU  - Živković, Suzana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6233
AB  - The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.
PB  - MDPI
T2  - Horticulturae
T1  - Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge
VL  - 9
IS  - 3
SP  - 381
DO  - 10.3390/horticulturae9030381
ER  - 

@article{
author = "Todorović, Slađana and Perić, Marija and Nikolić, Biljana and Mandić, Boris and Cvetković, Stefana and Bogdanović, Milica and Živković, Suzana",
year = "2023",
abstract = "The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge",
volume = "9",
number = "3",
pages = "381",
doi = "10.3390/horticulturae9030381"
}

Todorović, S., Perić, M., Nikolić, B., Mandić, B., Cvetković, S., Bogdanović, M.,& Živković, S.. (2023). Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae
MDPI., 9(3), 381.
https://doi.org/10.3390/horticulturae9030381

Todorović S, Perić M, Nikolić B, Mandić B, Cvetković S, Bogdanović M, Živković S. Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge. in Horticulturae. 2023;9(3):381.
doi:10.3390/horticulturae9030381 .

Todorović, Slađana, Perić, Marija, Nikolić, Biljana, Mandić, Boris, Cvetković, Stefana, Bogdanović, Milica, Živković, Suzana, "Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge" in Horticulturae, 9, no. 3 (2023):381,
https://doi.org/10.3390/horticulturae9030381 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš M.
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5896
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - MDPI
T2  - Antioxidants
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
VL  - 12
IS  - 2
SP  - 346
DO  - 10.3390/antiox12020346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš M. and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "MDPI",
journal = "Antioxidants, Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
volume = "12",
number = "2",
pages = "346",
doi = "10.3390/antiox12020346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U. M., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić UM, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš M., Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - JOUR
AU  - Kazimir, Aleksandr
AU  - Schwarze, Benedikt
AU  - Lönnecke, Peter
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Hey-Hawkins, Evamarie
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5892
AB  - The luminal A-subtype of breast cancer, where the oestrogen receptor α (ERα) is overexpressed, is the most frequent one. The prodrug tamoxifen (1) is the clinically used agent, inhibiting the ERα activity via the formation of several active metabolites, such as 4-hydroxytamoxifen (2) or 4,4′-dihydroxytamoxifen (3). In this study, we present the tamoxifen derivative 4-[1,1-bis(4-methoxyphenyl)but-1-en-2-yl]-2,2′-bipyridine (4), which was combined with platinum or palladium dichloride, the former a well-known scaffold in anticancer treatment, to give [PtCl2(4-κ2N,N′)] (5) or [PdCl2(4-κ2N,N′] (6). To prevent fast exchange of weakly coordinating chlorido ligands in aqueous solution, a bulky, highly stable and hydrophobic nido-carborate(−2) ([C2B9H11]2−) was incorporated. The resulting complexes [3-(4-κ2N,N′)-3,1,2-PtC2B9H11] (7) and [3-(4-κ2N,N′)-3,1,2-PdC2B9H11] (8) exhibit a dramatic change in electronic and biological properties compared to 5 and 6. Thus, 8 is highly selective for triple-negative MDA-MB-231 cells (IC50 = 3.7 μM, MTT test), while 7 is completely inactive against this cell line. The observed cytotoxicity of compounds 4–6 and 8 against this triple-negative cell line suggests off-target mechanisms rather than only ERα inhibition, for which these compounds were originally designed. Spectroscopic properties and electronic structures of the metal complexes were investigated for possible explanations of the biological activities.
PB  - MDPI
T2  - Pharmaceutics
T1  - Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes
VL  - 15
IS  - 2
SP  - 682
DO  - 10.3390/pharmaceutics15020682
ER  - 

@article{
author = "Kazimir, Aleksandr and Schwarze, Benedikt and Lönnecke, Peter and Jelača, Sanja and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Hey-Hawkins, Evamarie",
year = "2023",
abstract = "The luminal A-subtype of breast cancer, where the oestrogen receptor α (ERα) is overexpressed, is the most frequent one. The prodrug tamoxifen (1) is the clinically used agent, inhibiting the ERα activity via the formation of several active metabolites, such as 4-hydroxytamoxifen (2) or 4,4′-dihydroxytamoxifen (3). In this study, we present the tamoxifen derivative 4-[1,1-bis(4-methoxyphenyl)but-1-en-2-yl]-2,2′-bipyridine (4), which was combined with platinum or palladium dichloride, the former a well-known scaffold in anticancer treatment, to give [PtCl2(4-κ2N,N′)] (5) or [PdCl2(4-κ2N,N′] (6). To prevent fast exchange of weakly coordinating chlorido ligands in aqueous solution, a bulky, highly stable and hydrophobic nido-carborate(−2) ([C2B9H11]2−) was incorporated. The resulting complexes [3-(4-κ2N,N′)-3,1,2-PtC2B9H11] (7) and [3-(4-κ2N,N′)-3,1,2-PdC2B9H11] (8) exhibit a dramatic change in electronic and biological properties compared to 5 and 6. Thus, 8 is highly selective for triple-negative MDA-MB-231 cells (IC50 = 3.7 μM, MTT test), while 7 is completely inactive against this cell line. The observed cytotoxicity of compounds 4–6 and 8 against this triple-negative cell line suggests off-target mechanisms rather than only ERα inhibition, for which these compounds were originally designed. Spectroscopic properties and electronic structures of the metal complexes were investigated for possible explanations of the biological activities.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes",
volume = "15",
number = "2",
pages = "682",
doi = "10.3390/pharmaceutics15020682"
}

Kazimir, A., Schwarze, B., Lönnecke, P., Jelača, S., Mijatović, S., Maksimović-Ivanić, D.,& Hey-Hawkins, E.. (2023). Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes. in Pharmaceutics
MDPI., 15(2), 682.
https://doi.org/10.3390/pharmaceutics15020682

Kazimir A, Schwarze B, Lönnecke P, Jelača S, Mijatović S, Maksimović-Ivanić D, Hey-Hawkins E. Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes. in Pharmaceutics. 2023;15(2):682.
doi:10.3390/pharmaceutics15020682 .

Kazimir, Aleksandr, Schwarze, Benedikt, Lönnecke, Peter, Jelača, Sanja, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Hey-Hawkins, Evamarie, "Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes" in Pharmaceutics, 15, no. 2 (2023):682,
https://doi.org/10.3390/pharmaceutics15020682 . .

TY  - DATA
AU  - Kazimir, Aleksandr
AU  - Schwarze, Benedikt
AU  - Lönnecke, Peter
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Hey-Hawkins, Evamarie
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5893
AB  - The luminal A-subtype of breast cancer, where the oestrogen receptor α (ERα) is overexpressed, is the most frequent one. The prodrug tamoxifen (1) is the clinically used agent, inhibiting the ERα activity via the formation of several active metabolites, such as 4-hydroxytamoxifen (2) or 4,4′-dihydroxytamoxifen (3). In this study, we present the tamoxifen derivative 4-[1,1-bis(4-methoxyphenyl)but-1-en-2-yl]-2,2′-bipyridine (4), which was combined with platinum or palladium dichloride, the former a well-known scaffold in anticancer treatment, to give [PtCl2(4-κ2N,N′)] (5) or [PdCl2(4-κ2N,N′] (6). To prevent fast exchange of weakly coordinating chlorido ligands in aqueous solution, a bulky, highly stable and hydrophobic nido-carborate(−2) ([C2B9H11]2−) was incorporated. The resulting complexes [3-(4-κ2N,N′)-3,1,2-PtC2B9H11] (7) and [3-(4-κ2N,N′)-3,1,2-PdC2B9H11] (8) exhibit a dramatic change in electronic and biological properties compared to 5 and 6. Thus, 8 is highly selective for triple-negative MDA-MB-231 cells (IC50 = 3.7 μM, MTT test), while 7 is completely inactive against this cell line. The observed cytotoxicity of compounds 4–6 and 8 against this triple-negative cell line suggests off-target mechanisms rather than only ERα inhibition, for which these compounds were originally designed. Spectroscopic properties and electronic structures of the metal complexes were investigated for possible explanations of the biological activities.
PB  - MDPI
T2  - Pharmaceutics
T1  - Supplementary materials for: Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes
VL  - 15
IS  - 2
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5893
ER  - 

@misc{
author = "Kazimir, Aleksandr and Schwarze, Benedikt and Lönnecke, Peter and Jelača, Sanja and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Hey-Hawkins, Evamarie",
year = "2023",
abstract = "The luminal A-subtype of breast cancer, where the oestrogen receptor α (ERα) is overexpressed, is the most frequent one. The prodrug tamoxifen (1) is the clinically used agent, inhibiting the ERα activity via the formation of several active metabolites, such as 4-hydroxytamoxifen (2) or 4,4′-dihydroxytamoxifen (3). In this study, we present the tamoxifen derivative 4-[1,1-bis(4-methoxyphenyl)but-1-en-2-yl]-2,2′-bipyridine (4), which was combined with platinum or palladium dichloride, the former a well-known scaffold in anticancer treatment, to give [PtCl2(4-κ2N,N′)] (5) or [PdCl2(4-κ2N,N′] (6). To prevent fast exchange of weakly coordinating chlorido ligands in aqueous solution, a bulky, highly stable and hydrophobic nido-carborate(−2) ([C2B9H11]2−) was incorporated. The resulting complexes [3-(4-κ2N,N′)-3,1,2-PtC2B9H11] (7) and [3-(4-κ2N,N′)-3,1,2-PdC2B9H11] (8) exhibit a dramatic change in electronic and biological properties compared to 5 and 6. Thus, 8 is highly selective for triple-negative MDA-MB-231 cells (IC50 = 3.7 μM, MTT test), while 7 is completely inactive against this cell line. The observed cytotoxicity of compounds 4–6 and 8 against this triple-negative cell line suggests off-target mechanisms rather than only ERα inhibition, for which these compounds were originally designed. Spectroscopic properties and electronic structures of the metal complexes were investigated for possible explanations of the biological activities.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Supplementary materials for: Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes",
volume = "15",
number = "2",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5893"
}

Kazimir, A., Schwarze, B., Lönnecke, P., Jelača, S., Mijatović, S., Maksimović-Ivanić, D.,& Hey-Hawkins, E.. (2023). Supplementary materials for: Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes. in Pharmaceutics
MDPI., 15(2).
https://hdl.handle.net/21.15107/rcub_cherry_5893

Kazimir A, Schwarze B, Lönnecke P, Jelača S, Mijatović S, Maksimović-Ivanić D, Hey-Hawkins E. Supplementary materials for: Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes. in Pharmaceutics. 2023;15(2).
https://hdl.handle.net/21.15107/rcub_cherry_5893 .

Kazimir, Aleksandr, Schwarze, Benedikt, Lönnecke, Peter, Jelača, Sanja, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Hey-Hawkins, Evamarie, "Supplementary materials for: Metallodrugs against Breast Cancer: Combining the Tamoxifen Vector with Platinum(II) and Palladium(II) Complexes" in Pharmaceutics, 15, no. 2 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5893 .

TY  - JOUR
AU  - Mikavica, Ivana
AU  - Ranđelović, Dragana
AU  - Đorđević, Vladan
AU  - Rakić, Tamara
AU  - Gajić, Gordana
AU  - Mutić, Jelena
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5516
AB  - The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types—cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.
T2  - Environmental Science and Pollution Research
T2  - Environmental Science and Pollution Research
T1  - Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types
VL  - n/a
DO  - 10.1007/s11356-022-22110-z
ER  - 

@article{
author = "Mikavica, Ivana and Ranđelović, Dragana and Đorđević, Vladan and Rakić, Tamara and Gajić, Gordana and Mutić, Jelena",
year = "2023",
abstract = "The mobility of chemical elements in the soil-orchid system has been poorly studied. The aim of this study is to evaluate the uptake and mobility of several trace (Li, Ba, Sr, Ag, Hg, and B) and macronutrients (Ca, Mg, and K) in the orchid Anacamptis morio (L.) R.M.Bateman, Pridgeon & M.W.Chase from soils in western Serbia. The sampling sites are characterized by three different bedrock types—cherts, limestones, and serpentines, which are the source of the significant chemical differences in the elemental status of the soil and plant tissues. The four-step Community Bureau of Reference sequential extraction procedure was used to determine the distribution of fractions and predict their potential phytoavailability. The orchid and soil samples were analyzed for total elemental content analysis using ICP-OES. The greatest potential for plant availability was determined for Ba and Sr, representing about 80% of the total soil content. More than 40% of Li in the soils was found to be potentially phytoavailable. Significant correlations were found between the total content of Li, B, and Sr in soils. Between 38 and 60% of Li content and more than 80% of Ba and Sr content were determined to be potentially phytoavailable by sequential analysis. The highest bioconcentration factor (> 1) was determined in the case of B and Sr for all orchid organs, while translocation factor for Li was highest in tubers and leaves. The studied elements were mainly stored in tubers and roots, indicating the exclusion strategy of A. morio as a metal tolerance mechanism. The data obtained showed significant differences in metal content in soils and plants originating from sites with different parent materials, suggesting that bedrock type and associated soil properties are important factors that determine chemical element mobility and uptake.",
journal = "Environmental Science and Pollution Research, Environmental Science and Pollution Research",
title = "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types",
volume = "n/a",
doi = "10.1007/s11356-022-22110-z"
}

Mikavica, I., Ranđelović, D., Đorđević, V., Rakić, T., Gajić, G.,& Mutić, J.. (2023). Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research, n/a.
https://doi.org/10.1007/s11356-022-22110-z

Mikavica I, Ranđelović D, Đorđević V, Rakić T, Gajić G, Mutić J. Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types. in Environmental Science and Pollution Research. 2023;n/a.
doi:10.1007/s11356-022-22110-z .

Mikavica, Ivana, Ranđelović, Dragana, Đorđević, Vladan, Rakić, Tamara, Gajić, Gordana, Mutić, Jelena, "Concentration and mobility of trace elements (Li, Ba, Sr, Ag, Hg, B) and macronutrients (Ca, Mg, K) in soil-orchid system on different bedrock types" in Environmental Science and Pollution Research, n/a (2023),
https://doi.org/10.1007/s11356-022-22110-z . .

TY  - JOUR
AU  - Fotirić-Akšić, Milica M.
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav Lj.
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš M.
AU  - Dojčinović, Biljana P.
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5654
AB  - Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth
PB  - MDPI
T2  - Horticulturae
T1  - Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions
VL  - 8
SP  - 765
DO  - 10.3390/horticulturae8090765
ER  - 

@article{
author = "Fotirić-Akšić, Milica M. and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav Lj. and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš M. and Dojčinović, Biljana P. and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth",
publisher = "MDPI",
journal = "Horticulturae",
title = "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions",
volume = "8",
pages = "765",
doi = "10.3390/horticulturae8090765"
}

Fotirić-Akšić, M. M., Nešović, M., Ćirić, I., Tešić, Ž. Lj., Pezo, L., Tosti, T., Gašić, U. M., Dojčinović, B. P., Lončar, B.,& Meland, M.. (2022). Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae
MDPI., 8, 765.
https://doi.org/10.3390/horticulturae8090765

Fotirić-Akšić MM, Nešović M, Ćirić I, Tešić ŽL, Pezo L, Tosti T, Gašić UM, Dojčinović BP, Lončar B, Meland M. Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae. 2022;8:765.
doi:10.3390/horticulturae8090765 .

Fotirić-Akšić, Milica M., Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav Lj., Pezo, Lato, Tosti, Tomislav, Gašić, Uroš M., Dojčinović, Biljana P., Lončar, Biljana, Meland, Mekjell, "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions" in Horticulturae, 8 (2022):765,
https://doi.org/10.3390/horticulturae8090765 . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Perović, Milan
AU  - Miković, Željko
AU  - Đurđić, Slađana Z.
AU  - Manojlović, Dragan D.
AU  - Stojsavljević, Aleksandar
PY  - 2022
UR  - https://www.mdpi.com/journal/ijms
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5522
AB  - With the ever-growing concern for human health and wellbeing, the prenatal period ofdevelopment requires special attention since fetuses can be exposed to various metals through themother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, andplacental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on thepotential transplacental transfer of these trace metals. Based on the obtained levels of investigatedelements in clinical samples, it was observed that all of the trace metals cross the placental barrierand reach the fetus. Furthermore, statistical analysis revealed significant differences in levels oftoxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinicalsamples. Correlation analysis highlighted As to be an element with levels that differed significantlybetween all tested samples. Principal component analysis (PCA) was used to enhance these findings.PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals indistinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible forthe clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera wasresponsible for the clustering of these samples. Overall, the findings of this study could contribute toa better understanding of transplacental transfer of these trace metals, and shed a light on overalllevels of metal exposure in the population of healthy pregnant women and their fetuses.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy
VL  - 23
IS  - 15
SP  - 8078
DO  - 10.3390/ijms23158078
ER  - 

@article{
author = "Jagodić, Jovana and Pavlović, Slađan and Borković-Mitić, Slavica and Perović, Milan and Miković, Željko and Đurđić, Slađana Z. and Manojlović, Dragan D. and Stojsavljević, Aleksandar",
year = "2022",
abstract = "With the ever-growing concern for human health and wellbeing, the prenatal period ofdevelopment requires special attention since fetuses can be exposed to various metals through themother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, andplacental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on thepotential transplacental transfer of these trace metals. Based on the obtained levels of investigatedelements in clinical samples, it was observed that all of the trace metals cross the placental barrierand reach the fetus. Furthermore, statistical analysis revealed significant differences in levels oftoxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinicalsamples. Correlation analysis highlighted As to be an element with levels that differed significantlybetween all tested samples. Principal component analysis (PCA) was used to enhance these findings.PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals indistinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible forthe clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera wasresponsible for the clustering of these samples. Overall, the findings of this study could contribute toa better understanding of transplacental transfer of these trace metals, and shed a light on overalllevels of metal exposure in the population of healthy pregnant women and their fetuses.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy",
volume = "23",
number = "15",
pages = "8078",
doi = "10.3390/ijms23158078"
}

Jagodić, J., Pavlović, S., Borković-Mitić, S., Perović, M., Miković, Ž., Đurđić, S. Z., Manojlović, D. D.,& Stojsavljević, A.. (2022). Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences
MDPI., 23(15), 8078.
https://doi.org/10.3390/ijms23158078

Jagodić J, Pavlović S, Borković-Mitić S, Perović M, Miković Ž, Đurđić SZ, Manojlović DD, Stojsavljević A. Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences. 2022;23(15):8078.
doi:10.3390/ijms23158078 .

Jagodić, Jovana, Pavlović, Slađan, Borković-Mitić, Slavica, Perović, Milan, Miković, Željko, Đurđić, Slađana Z., Manojlović, Dragan D., Stojsavljević, Aleksandar, "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy" in International Journal of Molecular Sciences, 23, no. 15 (2022):8078,
https://doi.org/10.3390/ijms23158078 . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš M.
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav Lj.
AU  - Vovk, Irena
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5505
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia
VL  - 9
SP  - 941463
DO  - 10.3389/fnut.2022.941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš M. and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav Lj. and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition, Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia",
volume = "9",
pages = "941463",
doi = "10.3389/fnut.2022.941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U. M., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž. Lj.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition
Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić UM, Baošić R, Joksimović K, Pezo L, Tešić ŽL, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš M., Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav Lj., Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia" in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Mijović, Milica
AU  - Nestorović, Vojkan
AU  - Mijušković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Spasić, Snežana
AU  - Blagojević, Duško
AU  - Miljević, Čedo
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5669
AB  - Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis
VL  - 23
SP  - 13698
DO  - 10.3390/ijms232213698
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Brkljačić, Jelena and Mijović, Milica and Nestorović, Vojkan and Mijušković, Ana and Oreščanin-Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Spasić, Snežana and Blagojević, Duško and Miljević, Čedo",
year = "2022",
abstract = "Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis",
volume = "23",
pages = "13698",
doi = "10.3390/ijms232213698"
}

Nikolić-Kokić, A., Tatalović, N., Brkljačić, J., Mijović, M., Nestorović, V., Mijušković, A., Oreščanin-Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Spasić, S., Blagojević, D.,& Miljević, Č.. (2022). Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences
MDPI., 23, 13698.
https://doi.org/10.3390/ijms232213698

Nikolić-Kokić A, Tatalović N, Brkljačić J, Mijović M, Nestorović V, Mijušković A, Oreščanin-Dušić Z, Vidonja Uzelac T, Nikolić M, Spasić S, Blagojević D, Miljević Č. Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences. 2022;23:13698.
doi:10.3390/ijms232213698 .

Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Brkljačić, Jelena, Mijović, Milica, Nestorović, Vojkan, Mijušković, Ana, Oreščanin-Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Spasić, Snežana, Blagojević, Duško, Miljević, Čedo, "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis" in International Journal of Molecular Sciences, 23 (2022):13698,
https://doi.org/10.3390/ijms232213698 . .

TY  - JOUR
AU  - Pavković‐Lučić, Sofija
AU  - Trajković, Jelena
AU  - Miličić, Dragana
AU  - Anđelković, Boban D.
AU  - Lučić, Luka
AU  - Savić, Tatjana
AU  - Vujisić, Ljubodrag V.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5019
AB  - n the world of complex smells in natural environment,feeding and mating represent two important olfactory‐guided behaviors inDrosophila melanogaster(Diptera:Drosophilidae). Diet affects the chemoprofile compositionof the individuals, which, indirectly, may significantly affecttheir mating success. In this study, chemoprofiles of re-cently mated flies belonging to fourD. melanogasterstrains,which were fed for many generations on different sub-strates (standard cornmeal—S strain; banana—B strain;carrot—C strain; tomato—T strain) were identified andquantified. In total, 67 chemical compounds were identi-fied: 48 compounds were extracted from males maintainedon banana and carrot, and 47 compounds from malesmaintained on cornmeal and tomato substrates, while totalof 60 compounds were identified in females from allstrains. The strains and the sexes significantly differed inqualitative nature of their chemoprofiles after mating. Sig-nificant differences in the relative amount of three majormale  pheromones  (cis‐vaccenyl  acetate—cVA,  (Z)‐7‐pentacosene, and (Z)‐7‐tricosene) and in female pheromone(Z,Z)‐7,11‐nonacosadiene among strains were also re-corded. Furthermore, multivariate analysis of variance(MANOVA) pointed to significant differences between irgin and mated individuals of all strains and within bothsexes. Differences in some of the well known sex pher-omones were also identified when comparing their relativeamount before and after mating. The presence of typicalmale pheromones in females, and vice versa may indicatetheir bidirectional transfer during copulation. Our resultsconfirm significant effect of mating status on cuticular hy-drocarbon (CHC) phenotypes in differently fedD. melano-gasterflies.
PB  - Wiley
T2  - Archives of Insect Biochemistry and Physiology
T1  - “Scent of a fruit fly”: Cuticular chemoprofilesafter mating in differently fedDrosophilamelanogaster(Diptera: Drosophilidae) strains
VL  - 109
IS  - 3
SP  - e21866
DO  - 10.1002/arch.21866
ER  - 

@article{
author = "Pavković‐Lučić, Sofija and Trajković, Jelena and Miličić, Dragana and Anđelković, Boban D. and Lučić, Luka and Savić, Tatjana and Vujisić, Ljubodrag V.",
year = "2022",
abstract = "n the world of complex smells in natural environment,feeding and mating represent two important olfactory‐guided behaviors inDrosophila melanogaster(Diptera:Drosophilidae). Diet affects the chemoprofile compositionof the individuals, which, indirectly, may significantly affecttheir mating success. In this study, chemoprofiles of re-cently mated flies belonging to fourD. melanogasterstrains,which were fed for many generations on different sub-strates (standard cornmeal—S strain; banana—B strain;carrot—C strain; tomato—T strain) were identified andquantified. In total, 67 chemical compounds were identi-fied: 48 compounds were extracted from males maintainedon banana and carrot, and 47 compounds from malesmaintained on cornmeal and tomato substrates, while totalof 60 compounds were identified in females from allstrains. The strains and the sexes significantly differed inqualitative nature of their chemoprofiles after mating. Sig-nificant differences in the relative amount of three majormale  pheromones  (cis‐vaccenyl  acetate—cVA,  (Z)‐7‐pentacosene, and (Z)‐7‐tricosene) and in female pheromone(Z,Z)‐7,11‐nonacosadiene among strains were also re-corded. Furthermore, multivariate analysis of variance(MANOVA) pointed to significant differences between irgin and mated individuals of all strains and within bothsexes. Differences in some of the well known sex pher-omones were also identified when comparing their relativeamount before and after mating. The presence of typicalmale pheromones in females, and vice versa may indicatetheir bidirectional transfer during copulation. Our resultsconfirm significant effect of mating status on cuticular hy-drocarbon (CHC) phenotypes in differently fedD. melano-gasterflies.",
publisher = "Wiley",
journal = "Archives of Insect Biochemistry and Physiology",
title = "“Scent of a fruit fly”: Cuticular chemoprofilesafter mating in differently fedDrosophilamelanogaster(Diptera: Drosophilidae) strains",
volume = "109",
number = "3",
pages = "e21866",
doi = "10.1002/arch.21866"
}

Pavković‐Lučić, S., Trajković, J., Miličić, D., Anđelković, B. D., Lučić, L., Savić, T.,& Vujisić, L. V.. (2022). “Scent of a fruit fly”: Cuticular chemoprofilesafter mating in differently fedDrosophilamelanogaster(Diptera: Drosophilidae) strains. in Archives of Insect Biochemistry and Physiology
Wiley., 109(3), e21866.
https://doi.org/10.1002/arch.21866

Pavković‐Lučić S, Trajković J, Miličić D, Anđelković BD, Lučić L, Savić T, Vujisić LV. “Scent of a fruit fly”: Cuticular chemoprofilesafter mating in differently fedDrosophilamelanogaster(Diptera: Drosophilidae) strains. in Archives of Insect Biochemistry and Physiology. 2022;109(3):e21866.
doi:10.1002/arch.21866 .

Pavković‐Lučić, Sofija, Trajković, Jelena, Miličić, Dragana, Anđelković, Boban D., Lučić, Luka, Savić, Tatjana, Vujisić, Ljubodrag V., "“Scent of a fruit fly”: Cuticular chemoprofilesafter mating in differently fedDrosophilamelanogaster(Diptera: Drosophilidae) strains" in Archives of Insect Biochemistry and Physiology, 109, no. 3 (2022):e21866,
https://doi.org/10.1002/arch.21866 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Vujisić, Ljubodrag V.
AU  - Todosijević, Marina
AU  - Stevanović, Nataša
AU  - Perić-Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Ćurčić, Srećko
PY  - 2022
UR  - https://doi.org/10.1007/s00114-022-01790-0
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5183
AB  - Pygidial gland secretions are used as repellent defensive allomones in ground beetles. We provide the first precise data on the chemical composition and antimicrobial potency of the secretion of the blue ground beetle, as well as on the morphology of its pygidial glands. The latter structures were not previously studied chemoecologically and morphologically, and we hypothesized that their secretion may have some antimicrobial action, as is the case with certain Carabus species. Gas chromatography-mass spectrometry (GC-MS) was used to identify methacrylic and angelic acids as dominant chemicals in the secretion from individuals of three populations of the blue ground beetle in Serbia. We tested its secretion against selected strains of medically important microorganisms. The secretion exibits antimicrobial action against certain bacterial species and all tested micromycetes. The most significant antifungal effect of the secretion was against Penicillium ochrochloron, which is more sensitive to the secretion than to commercial antifungal drugs ketoconazole and bifonazole. Bifonazole achieved minimum inhibitory concentrations against Trichoderma viride at more than three times higher value than did the secretion, indicating a significant antifungal effect of the secretion against this micromycete as well. Additionally, we tested commercially available standards of two dominant chemicals in the secretion to investigate their interaction and antimicrobial role in the secretion. Finally, we describe all glandular morpho-functional units of the blue ground beetle. Our results suggest that the secretion of the blue ground beetle may serve not only defensive but also antimicrobial functions, which likely aid the survival of this beetle in the microbial-rich forest litter habitat.
PB  - SpringerLink
T2  - The Science of Nature
T1  - Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity
VL  - 109
IS  - 2
SP  - 19
DO  - 10.1007/s00114-022-01790-0
ER  - 

@article{
author = "Vesović, Nikola and Nenadić, Marija and Soković, Marina and Ćirić, Ana and Vujisić, Ljubodrag V. and Todosijević, Marina and Stevanović, Nataša and Perić-Mataruga, Vesna and Ilijin, Larisa and Ćurčić, Srećko",
year = "2022",
abstract = "Pygidial gland secretions are used as repellent defensive allomones in ground beetles. We provide the first precise data on the chemical composition and antimicrobial potency of the secretion of the blue ground beetle, as well as on the morphology of its pygidial glands. The latter structures were not previously studied chemoecologically and morphologically, and we hypothesized that their secretion may have some antimicrobial action, as is the case with certain Carabus species. Gas chromatography-mass spectrometry (GC-MS) was used to identify methacrylic and angelic acids as dominant chemicals in the secretion from individuals of three populations of the blue ground beetle in Serbia. We tested its secretion against selected strains of medically important microorganisms. The secretion exibits antimicrobial action against certain bacterial species and all tested micromycetes. The most significant antifungal effect of the secretion was against Penicillium ochrochloron, which is more sensitive to the secretion than to commercial antifungal drugs ketoconazole and bifonazole. Bifonazole achieved minimum inhibitory concentrations against Trichoderma viride at more than three times higher value than did the secretion, indicating a significant antifungal effect of the secretion against this micromycete as well. Additionally, we tested commercially available standards of two dominant chemicals in the secretion to investigate their interaction and antimicrobial role in the secretion. Finally, we describe all glandular morpho-functional units of the blue ground beetle. Our results suggest that the secretion of the blue ground beetle may serve not only defensive but also antimicrobial functions, which likely aid the survival of this beetle in the microbial-rich forest litter habitat.",
publisher = "SpringerLink",
journal = "The Science of Nature",
title = "Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity",
volume = "109",
number = "2",
pages = "19",
doi = "10.1007/s00114-022-01790-0"
}

Vesović, N., Nenadić, M., Soković, M., Ćirić, A., Vujisić, L. V., Todosijević, M., Stevanović, N., Perić-Mataruga, V., Ilijin, L.,& Ćurčić, S.. (2022). Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity. in The Science of Nature
SpringerLink., 109(2), 19.
https://doi.org/10.1007/s00114-022-01790-0

Vesović N, Nenadić M, Soković M, Ćirić A, Vujisić LV, Todosijević M, Stevanović N, Perić-Mataruga V, Ilijin L, Ćurčić S. Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity. in The Science of Nature. 2022;109(2):19.
doi:10.1007/s00114-022-01790-0 .

Vesović, Nikola, Nenadić, Marija, Soković, Marina, Ćirić, Ana, Vujisić, Ljubodrag V., Todosijević, Marina, Stevanović, Nataša, Perić-Mataruga, Vesna, Ilijin, Larisa, Ćurčić, Srećko, "Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity" in The Science of Nature, 109, no. 2 (2022):19,
https://doi.org/10.1007/s00114-022-01790-0 . .

TY  - JOUR
AU  - Pavković-Lučić, Sofija
AU  - Trajković, Jelena
AU  - Miličić, Dragana
AU  - Anđelković, Boban
AU  - Lučić, Luka
AU  - Savić, Tatjana
AU  - Vujisić, Ljubodrag V.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5978
AB  - In the world of complex smells in natural environment, feeding and mating represent two important olfactory-guided behaviors in Drosophila melanogaster (Diptera: Drosophilidae). Diet affects the chemoprofile composition of the individuals, which, indirectly, may significantly affect their mating success. In this study, chemoprofiles of recently mated flies belonging to four D. melanogaster strains, which were fed for many generations on different substrates (standard cornmeal-S strain; banana-B strain; carrot-C strain; tomato-T strain) were identified and quantified. In total, 67 chemical compounds were identified: 48 compounds were extracted from males maintained on banana and carrot, and 47 compounds from males maintained on cornmeal and tomato substrates, while total of 60 compounds were identified in females from all strains. The strains and the sexes significantly differed in qualitative nature of their chemoprofiles after mating. Significant differences in the relative amount of three major male pheromones (cis-vaccenyl acetate-cVA, (Z)-7-pentacosene, and (Z)-7-tricosene) and in female pheromone (Z,Z)-7,11-nonacosadiene among strains were also recorded. Furthermore, multivariate analysis of variance (MANOVA) pointed to significant differences between virgin and mated individuals of all strains and within both sexes. Differences in some of the well known sex pheromones were also identified when comparing their relative amount before and after mating. The presence of typical male pheromones in females, and vice versa may indicate their bidirectional transfer during copulation. Our results confirm significant effect of mating status on cuticular hydrocarbon (CHC) phenotypes in differently fed D. melanogaster flies.
PB  - Hoboken: John Wiley and Sons Inc.
T2  - Archives of Insect Biochemistry and Physiology
T2  - Archives of Insect Biochemistry and Physiology
T1  - "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.
SP  - e21866
DO  - 10.1002/arch.21866
ER  - 

@article{
author = "Pavković-Lučić, Sofija and Trajković, Jelena and Miličić, Dragana and Anđelković, Boban and Lučić, Luka and Savić, Tatjana and Vujisić, Ljubodrag V.",
year = "2022",
abstract = "In the world of complex smells in natural environment, feeding and mating represent two important olfactory-guided behaviors in Drosophila melanogaster (Diptera: Drosophilidae). Diet affects the chemoprofile composition of the individuals, which, indirectly, may significantly affect their mating success. In this study, chemoprofiles of recently mated flies belonging to four D. melanogaster strains, which were fed for many generations on different substrates (standard cornmeal-S strain; banana-B strain; carrot-C strain; tomato-T strain) were identified and quantified. In total, 67 chemical compounds were identified: 48 compounds were extracted from males maintained on banana and carrot, and 47 compounds from males maintained on cornmeal and tomato substrates, while total of 60 compounds were identified in females from all strains. The strains and the sexes significantly differed in qualitative nature of their chemoprofiles after mating. Significant differences in the relative amount of three major male pheromones (cis-vaccenyl acetate-cVA, (Z)-7-pentacosene, and (Z)-7-tricosene) and in female pheromone (Z,Z)-7,11-nonacosadiene among strains were also recorded. Furthermore, multivariate analysis of variance (MANOVA) pointed to significant differences between virgin and mated individuals of all strains and within both sexes. Differences in some of the well known sex pheromones were also identified when comparing their relative amount before and after mating. The presence of typical male pheromones in females, and vice versa may indicate their bidirectional transfer during copulation. Our results confirm significant effect of mating status on cuticular hydrocarbon (CHC) phenotypes in differently fed D. melanogaster flies.",
publisher = "Hoboken: John Wiley and Sons Inc.",
journal = "Archives of Insect Biochemistry and Physiology, Archives of Insect Biochemistry and Physiology",
title = ""Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.",
pages = "e21866",
doi = "10.1002/arch.21866"
}

Pavković-Lučić, S., Trajković, J., Miličić, D., Anđelković, B., Lučić, L., Savić, T.,& Vujisić, L. V.. (2022). "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.. in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons Inc.., e21866.
https://doi.org/10.1002/arch.21866

Pavković-Lučić S, Trajković J, Miličić D, Anđelković B, Lučić L, Savić T, Vujisić LV. "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.. in Archives of Insect Biochemistry and Physiology. 2022;:e21866.
doi:10.1002/arch.21866 .

Pavković-Lučić, Sofija, Trajković, Jelena, Miličić, Dragana, Anđelković, Boban, Lučić, Luka, Savić, Tatjana, Vujisić, Ljubodrag V., ""Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains." in Archives of Insect Biochemistry and Physiology (2022):e21866,
https://doi.org/10.1002/arch.21866 . .

TY  - JOUR
AU  - Brčić-Karačonji, Irena
AU  - Jurica, Karlo
AU  - Gašić, Uroš M.
AU  - Dramićanin, Aleksandra M.
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5014
AB  - The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.
PB  - MDPI
T2  - Plants
T1  - Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
VL  - 11
IS  - 1
SP  - 25
DO  - 10.3390/plants11010025
ER  - 

@article{
author = "Brčić-Karačonji, Irena and Jurica, Karlo and Gašić, Uroš M. and Dramićanin, Aleksandra M. and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean
shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial,
antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the
presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total
phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected
at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid
chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was
determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of
64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside
and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were
the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and
fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed
that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate
samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of
67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA
for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 µmol Trolox equivalents
(TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves.
Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to
A. unedo as a promising health-promoting plant and natural food preservative.",
publisher = "MDPI",
journal = "Plants",
title = "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits",
volume = "11",
number = "1",
pages = "25",
doi = "10.3390/plants11010025"
}

Brčić-Karačonji, I., Jurica, K., Gašić, U. M., Dramićanin, A. M., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2022). Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants
MDPI., 11(1), 25.
https://doi.org/10.3390/plants11010025

Brčić-Karačonji I, Jurica K, Gašić UM, Dramićanin AM, Tešić ŽL, Milojković-Opsenica D. Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits. in Plants. 2022;11(1):25.
doi:10.3390/plants11010025 .

Brčić-Karačonji, Irena, Jurica, Karlo, Gašić, Uroš M., Dramićanin, Aleksandra M., Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits" in Plants, 11, no. 1 (2022):25,
https://doi.org/10.3390/plants11010025 . .

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Ćirković, Bratislav
AU  - Laketić, Darko
AU  - Jovanović, Zoran
AU  - Kostić, Boban
AU  - Bešlić, Zoran
AU  - Sredojević, Milica
AU  - Tešić, Živoslav Lj.
AU  - Banjanac, Tijana
AU  - Gašić, Uroš M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5317
AB  - The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which
produces quality wines of characteristic and unique tastes. In the agroecological conditions of the
Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine
produced from the variety Prokupac was examined. Four experimental treatments with different
timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—
treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine
was determined for the three treatments of defoliation and the control treatment. Additionally,
a multivariate analysis was applied on the obtained results, together with already published data
(grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of
phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained,
while the treatments with early defoliation did not differ in relation to the control sample. Early
defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring
the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins
(TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by
the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.
PB  - MDPI
T2  - Horticulturae
T1  - Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)
VL  - 8
SP  - 296
DO  - 10.3390/horticulturae8040296
ER  - 

@article{
author = "Ćirković, Dušica and Matijašević, Saša and Ćirković, Bratislav and Laketić, Darko and Jovanović, Zoran and Kostić, Boban and Bešlić, Zoran and Sredojević, Milica and Tešić, Živoslav Lj. and Banjanac, Tijana and Gašić, Uroš M.",
year = "2022",
abstract = "The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which
produces quality wines of characteristic and unique tastes. In the agroecological conditions of the
Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine
produced from the variety Prokupac was examined. Four experimental treatments with different
timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—
treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine
was determined for the three treatments of defoliation and the control treatment. Additionally,
a multivariate analysis was applied on the obtained results, together with already published data
(grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of
phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained,
while the treatments with early defoliation did not differ in relation to the control sample. Early
defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring
the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins
(TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by
the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)",
volume = "8",
pages = "296",
doi = "10.3390/horticulturae8040296"
}

Ćirković, D., Matijašević, S., Ćirković, B., Laketić, D., Jovanović, Z., Kostić, B., Bešlić, Z., Sredojević, M., Tešić, Ž. Lj., Banjanac, T.,& Gašić, U. M.. (2022). Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae
MDPI., 8, 296.
https://doi.org/10.3390/horticulturae8040296

Ćirković D, Matijašević S, Ćirković B, Laketić D, Jovanović Z, Kostić B, Bešlić Z, Sredojević M, Tešić ŽL, Banjanac T, Gašić UM. Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae. 2022;8:296.
doi:10.3390/horticulturae8040296 .

Ćirković, Dušica, Matijašević, Saša, Ćirković, Bratislav, Laketić, Darko, Jovanović, Zoran, Kostić, Boban, Bešlić, Zoran, Sredojević, Milica, Tešić, Živoslav Lj., Banjanac, Tijana, Gašić, Uroš M., "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)" in Horticulturae, 8 (2022):296,
https://doi.org/10.3390/horticulturae8040296 . .

TY  - JOUR
AU  - Ljoljić Bilić, Vanja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Rimac, Hrvoje
AU  - Vuković Rodriguez, Jadranka
AU  - Vlainić, Josipa
AU  - Brlek‐Gorski, Diana
AU  - Kosalec, Ivan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5197
AB  - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation
PB  - MDPI
T2  - Аntibiotics
T1  - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
VL  - 11
SP  - 492
DO  - 10.3390/antibiotics11040492
ER  - 

@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek‐Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation",
publisher = "MDPI",
journal = "Аntibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
volume = "11",
pages = "492",
doi = "10.3390/antibiotics11040492"
}

Ljoljić Bilić, V., Gašić, U. M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek‐Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics
MDPI., 11, 492.
https://doi.org/10.3390/antibiotics11040492

Ljoljić Bilić V, Gašić UM, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek‐Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics. 2022;11:492.
doi:10.3390/antibiotics11040492 .

Ljoljić Bilić, Vanja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek‐Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Аntibiotics, 11 (2022):492,
https://doi.org/10.3390/antibiotics11040492 . .

TY  - JOUR
AU  - Jurič, Andreja
AU  - Brčić Karačonji, Irena
AU  - Žunec, Suzana
AU  - Katić, Anja
AU  - Gašić, Uroš M.
AU  - Milojković-Opsenica, Dušanka
AU  - Kopjar, Nevenka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5199
AB  - Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro
SP  - 1
EP  - 10
DO  - 10.1080/00218839.2022.2047421
ER  - 

@article{
author = "Jurič, Andreja and Brčić Karačonji, Irena and Žunec, Suzana and Katić, Anja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Kopjar, Nevenka",
year = "2022",
abstract = "Strawberry tree (Arbutus unedo L.) honey (STH) is widely used as part of traditional medicine
in the Mediterranean area, especially in the treatment of wounds, burns and infections. In
this study, we investigated the cyto-/genoprotective properties of STH at cell level following
short-term exposure to UVB radiation at 2 kJ/m2. Isolated human peripheral blood lymphocytes
(PBL) were selected as model cells. Phenolics in STH were determined using an ultrahigh-
performance liquid chromatograph (UHPLC) coupled to a linear ion trap-Orbitrap hybrid
mass spectrometer (LTQ Orbitrap MS), while homogentisic acid (HGA) was quantified using a
gas chromatograph-mass spectrometer (GC-MS). Primary DNA damage in PBLs treated with
STH before and after irradiation was assessed by alkaline comet assay, while their viability
was determined using the apoptosis/necrosis assay. The levels of thiobarbituric acid reactive
substances (TBARS) and glutathione (GSH) were determined in plasma samples. STH applied
as pre-treatment at a concentration equivalent to its average daily portion efficiently counteracted
the cytotoxic and genotoxic effects and diminished UVB-induced oxidative stress.
Post-radiation treatment with STH was not as beneficial. Protective effects could be associated
to the complex phytochemical profile of STH and the high content of HGA (306.8mg/
kg). It was confirmed that an intake of a complex mixture of bioactive constituents as STH
prior to potential cyto/genotoxic insults stimulated a broad-spectrum of protective mechanisms,
including intracellular antioxidative defence and DNA repair systems that helped pretreated
cells to counteract harmful exposures more efficiently.",
publisher = "Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro",
pages = "1-10",
doi = "10.1080/00218839.2022.2047421"
}

Jurič, A., Brčić Karačonji, I., Žunec, S., Katić, A., Gašić, U. M., Milojković-Opsenica, D.,& Kopjar, N.. (2022). Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research
Taylor and Francis., 1-10.
https://doi.org/10.1080/00218839.2022.2047421

Jurič A, Brčić Karačonji I, Žunec S, Katić A, Gašić UM, Milojković-Opsenica D, Kopjar N. Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro. in Journal of Apicultural Research. 2022;:1-10.
doi:10.1080/00218839.2022.2047421 .

Jurič, Andreja, Brčić Karačonji, Irena, Žunec, Suzana, Katić, Anja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Kopjar, Nevenka, "Protective role of strawberry tree (Arbutus unedo L.) honey against cyto/genotoxic effects induced by ultraviolet B radiation in vitro" in Journal of Apicultural Research (2022):1-10,
https://doi.org/10.1080/00218839.2022.2047421 . .

TY  - JOUR
AU  - Fotirić-Akšić, Milica M.
AU  - Dabić-Zagorac, Dragana
AU  - Gašić, Uroš M.
AU  - Tosti, Tomislav
AU  - Natić, Maja
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.mdpi.com/2071-1050/14/9/5300
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5178
AB  - The aim of this study was to compare total phenolic content (TPC), radical-scavenging activity (RSA), total anthocyanin content (TAC), sugar and polyphenolic profiles of two apple cultivars (‘Discovery’ and ‘Red Aroma Orelind’) from organic and integrated production systems in climatic conditions of Western Norway. Sixteen sugars and four sugar alcohols and 19 polyphenols were found in the peel, but less polyphenols were detected in the pulp. The peel of both apples and in both production systems had significantly higher TPC and RSA than the pulp. The peel from integrated apples had higher TPC than the peel from organic apples, while organic apples had higher TAC than the integrated. Sucrose and glucose levels were higher in organic apples; fructose was cultivar dependent while minor sugars were higher in integrated fruits. The most abundant polyphenolic compound in the peel of the tested cultivars was quercetin 3-O-galactoside, while chlorogenic acid was most abundant in the pulp. Regarding polyphenols, phloretin, phloridzin, protocatechuic acid, baicalein and naringenin were higher in organic apple, while quercetin 3-O-galactoside, kaempferol 3-O-glucoside, chlorogenic acid and syringic acid was higher in integrated fruits. In conclusion, organic ‘Discovery’ and integrated ‘Red Aroma Orelind’ had higher bioavailability of health related compounds from the peel and the pulp.
PB  - MDPI
T2  - Sustainability
T2  - Sustainability
T1  - Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems
VL  - 14
IS  - 9
SP  - 5300
DO  - 10.3390/su14095300
ER  - 

@article{
author = "Fotirić-Akšić, Milica M. and Dabić-Zagorac, Dragana and Gašić, Uroš M. and Tosti, Tomislav and Natić, Maja and Meland, Mekjell",
year = "2022",
abstract = "The aim of this study was to compare total phenolic content (TPC), radical-scavenging activity (RSA), total anthocyanin content (TAC), sugar and polyphenolic profiles of two apple cultivars (‘Discovery’ and ‘Red Aroma Orelind’) from organic and integrated production systems in climatic conditions of Western Norway. Sixteen sugars and four sugar alcohols and 19 polyphenols were found in the peel, but less polyphenols were detected in the pulp. The peel of both apples and in both production systems had significantly higher TPC and RSA than the pulp. The peel from integrated apples had higher TPC than the peel from organic apples, while organic apples had higher TAC than the integrated. Sucrose and glucose levels were higher in organic apples; fructose was cultivar dependent while minor sugars were higher in integrated fruits. The most abundant polyphenolic compound in the peel of the tested cultivars was quercetin 3-O-galactoside, while chlorogenic acid was most abundant in the pulp. Regarding polyphenols, phloretin, phloridzin, protocatechuic acid, baicalein and naringenin were higher in organic apple, while quercetin 3-O-galactoside, kaempferol 3-O-glucoside, chlorogenic acid and syringic acid was higher in integrated fruits. In conclusion, organic ‘Discovery’ and integrated ‘Red Aroma Orelind’ had higher bioavailability of health related compounds from the peel and the pulp.",
publisher = "MDPI",
journal = "Sustainability, Sustainability",
title = "Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems",
volume = "14",
number = "9",
pages = "5300",
doi = "10.3390/su14095300"
}

Fotirić-Akšić, M. M., Dabić-Zagorac, D., Gašić, U. M., Tosti, T., Natić, M.,& Meland, M.. (2022). Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems. in Sustainability
MDPI., 14(9), 5300.
https://doi.org/10.3390/su14095300

Fotirić-Akšić MM, Dabić-Zagorac D, Gašić UM, Tosti T, Natić M, Meland M. Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems. in Sustainability. 2022;14(9):5300.
doi:10.3390/su14095300 .

Fotirić-Akšić, Milica M., Dabić-Zagorac, Dragana, Gašić, Uroš M., Tosti, Tomislav, Natić, Maja, Meland, Mekjell, "Analysis of Apple Fruit (Malus × domestica Borkh.) Quality Attributes Obtained from Organic and Integrated Production Systems" in Sustainability, 14, no. 9 (2022):5300,
https://doi.org/10.3390/su14095300 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Mačukanović-Jocić, Marina P.
AU  - Milinčić, Danijel D.
AU  - Petrović, Jovana D.
AU  - Gašić, Uroš M.
AU  - Gligorijević, Nevenka N.
AU  - Jarić, Snežana V.
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35213785
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5203
AB  - The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents
VL  - 19
IS  - 4
SP  - e202200069
DO  - 10.1002/cbdv.202200069
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Mačukanović-Jocić, Marina P. and Milinčić, Danijel D. and Petrović, Jovana D. and Gašić, Uroš M. and Gligorijević, Nevenka N. and Jarić, Snežana V. and Soković, Marina and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents",
volume = "19",
number = "4",
pages = "e202200069",
doi = "10.1002/cbdv.202200069"
}

Kostić, A. Ž., Mačukanović-Jocić, M. P., Milinčić, D. D., Petrović, J. D., Gašić, U. M., Gligorijević, N. N., Jarić, S. V., Soković, M., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents. in Chemistry and Biodiversity
Wiley., 19(4), e202200069.
https://doi.org/10.1002/cbdv.202200069

Kostić AŽ, Mačukanović-Jocić MP, Milinčić DD, Petrović JD, Gašić UM, Gligorijević NN, Jarić SV, Soković M, Tešić ŽL, Pešić MB. Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents. in Chemistry and Biodiversity. 2022;19(4):e202200069.
doi:10.1002/cbdv.202200069 .

Kostić, Aleksandar Ž., Mačukanović-Jocić, Marina P., Milinčić, Danijel D., Petrović, Jovana D., Gašić, Uroš M., Gligorijević, Nevenka N., Jarić, Snežana V., Soković, Marina, Tešić, Živoslav Lj., Pešić, Mirjana B., "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents" in Chemistry and Biodiversity, 19, no. 4 (2022):e202200069,
https://doi.org/10.1002/cbdv.202200069 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav Lj.
AU  - Gašić, Uroš M.
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S2210803322000495
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5410
AB  - IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
PB  - Elsevier
T2  - Journal of Herbal Medicine
T2  - Journal of Herbal MedicineJournal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
SP  - 100580
DO  - 10.1016/j.hermed.2022.100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav Lj. and Gašić, Uroš M.",
year = "2022",
abstract = "IntroductionThe aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value.MethodsFor determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction.ResultsThe extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg).ConclusionsHoney, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
publisher = "Elsevier",
journal = "Journal of Herbal Medicine, Journal of Herbal MedicineJournal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
pages = "100580",
doi = "10.1016/j.hermed.2022.100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž. Lj.,& Gašić, U. M.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine
Elsevier., 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić ŽL, Gašić UM. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav Lj., Gašić, Uroš M., "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Milinčić, Danijel D.
AU  - Stanisavljević, Nemanja S.
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš M.
AU  - Stanojević, Slađana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5684
AB  - This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.
PB  - MDPI
T2  - Antioxidants
T1  - Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion
VL  - 11
IS  - 11
SP  - 2164
DO  - 10.3390/antiox11112164
ER  - 

@article{
author = "Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Kostić, Aleksandar Ž. and Gašić, Uroš M. and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion",
volume = "11",
number = "11",
pages = "2164",
doi = "10.3390/antiox11112164"
}

Milinčić, D. D., Stanisavljević, N. S., Kostić, A. Ž., Gašić, U. M., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants
MDPI., 11(11), 2164.
https://doi.org/10.3390/antiox11112164

Milinčić DD, Stanisavljević NS, Kostić AŽ, Gašić UM, Stanojević SP, Tešić ŽL, Pešić MB. Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants. 2022;11(11):2164.
doi:10.3390/antiox11112164 .

Milinčić, Danijel D., Stanisavljević, Nemanja S., Kostić, Aleksandar Ž., Gašić, Uroš M., Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion" in Antioxidants, 11, no. 11 (2022):2164,
https://doi.org/10.3390/antiox11112164 . .

TY  - JOUR
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš M.
AU  - Đurović, Dejan
AU  - Tešić, Živoslav Lj.
AU  - Fotirić-Akšić, Milica M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5683
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - MDPI
T2  - Molecules
T1  - Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia
VL  - 27
IS  - 21
SP  - 7651
DO  - 10.3390/molecules27217651
ER  - 

@article{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš M. and Đurović, Dejan and Tešić, Živoslav Lj. and Fotirić-Akšić, Milica M. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "MDPI",
journal = "Molecules",
title = "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia",
volume = "27",
number = "21",
pages = "7651",
doi = "10.3390/molecules27217651"
}

Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž. Lj., Fotirić-Akšić, M. M.,& Milojković-Opsenica, D.. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules
MDPI., 27(21), 7651.
https://doi.org/10.3390/molecules27217651

Horvacki N, Andrić F, Gašić UM, Đurović D, Tešić ŽL, Fotirić-Akšić MM, Milojković-Opsenica D. Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. in Molecules. 2022;27(21):7651.
doi:10.3390/molecules27217651 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš M., Đurović, Dejan, Tešić, Živoslav Lj., Fotirić-Akšić, Milica M., Milojković-Opsenica, Dušanka, "Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia" in Molecules, 27, no. 21 (2022):7651,
https://doi.org/10.3390/molecules27217651 . .

TY  - DATA
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš M.
AU  - Đurović, Dejan
AU  - Tešić, Živoslav Lj.
AU  - Fotirić-Akšić, Milica M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5701
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - MDPI
T2  - Molecules
T1  - Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651
VL  - 27
IS  - 21
SP  - 7651
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5701
ER  - 

@misc{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš M. and Đurović, Dejan and Tešić, Živoslav Lj. and Fotirić-Akšić, Milica M. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "MDPI",
journal = "Molecules",
title = "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651",
volume = "27",
number = "21",
pages = "7651",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5701"
}

Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž. Lj., Fotirić-Akšić, M. M.,& Milojković-Opsenica, D.. (2022). Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules
MDPI., 27(21), 7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701

Horvacki N, Andrić F, Gašić UM, Đurović D, Tešić ŽL, Fotirić-Akšić MM, Milojković-Opsenica D. Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules. 2022;27(21):7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701 .

Horvacki, Nikola, Andrić, Filip, Gašić, Uroš M., Đurović, Dejan, Tešić, Živoslav Lj., Fotirić-Akšić, Milica M., Milojković-Opsenica, Dušanka, "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651" in Molecules, 27, no. 21 (2022):7651,
https://hdl.handle.net/21.15107/rcub_cherry_5701 .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Ćirić, Ana
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Anđelković, Boban D.
AU  - Todosijević, Marina
AU  - Todorović, Slađana
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Tešević, Vele
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Kanellis, Angelos K.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5792
AB  - Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites, such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus. Histochemical staining of leaves’ trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus, grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive bacteria (Bacillus cereus and Micrococcus flavus). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent-3β-acetoxy-13-epi-manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent-3β-acetoxy-13-epi-manoyl oxide and its precursor ent-3β-hydroxy-13-epi-manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.
T2  - Industrial Crops and Products
T2  - Industrial Crops and ProductsIndustrial Crops and Products
T1  - Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”
VL  - 184
SP  - 114992
DO  - 10.1016/j.indcrop.2022.114992
ER  - 

@article{
author = "Skorić, Marijana and Ćirić, Ana and Budimir, Snežana and Janošević, Dušica and Anđelković, Boban D. and Todosijević, Marina and Todorović, Slađana and Soković, Marina and Glamočlija, Jasmina and Tešević, Vele and Gašić, Uroš M. and Mišić, Danijela and Kanellis, Angelos K.",
year = "2022",
abstract = "Cistus creticus subsp. creticus is a shrubby Mediterranean plant used since ancient times in folk medicine for the treatment of different diseases. C. creticus extracts and resin contain different types of secondary metabolites, such as terpenoids (predominantly labdane type diterpenoids), and phenylpropanoids. Growth conditions seem to influence the content of labdane-type diterpenes and flavan-3-ols in leaves of C. creticus subsp. creticus. Histochemical staining of leaves’ trichomes and comprehensive phytochemical characterization of resin, leaves and their exudates, indicated that long-stalked capitate trichomes of C. creticus subsp. creticus, grown both in vitro (IV) and in greenhouse (GH), are capable of producing bioactive oleoresin-related terpenoids and phenylpropanoids compounds. Bioactivity-guided approach was implemented in search for the major antibacterial compound in C. creticus resin against two Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and two Gram-positive bacteria (Bacillus cereus and Micrococcus flavus). Bioautographic assay on TLC plates with separated components of Cistus resin extract, revealed a pronounced zone of microbial growth inhibition, corresponded to a highly active compound with Rf values of 0.45, structurally characterized and identified as ent-3β-acetoxy-13-epi-manoyl oxide. This finding opens the route for focusing on isolation and functional characterization of genes involved in the biosynthesis of ent-3β-acetoxy-13-epi-manoyl oxide and its precursor ent-3β-hydroxy-13-epi-manoyl oxide, with the aim to establish sustainable in vitro biotechnological protocols for its large-scale production in homologous and heterologous plant and microbial hosts.",
journal = "Industrial Crops and Products, Industrial Crops and ProductsIndustrial Crops and Products",
title = "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”",
volume = "184",
pages = "114992",
doi = "10.1016/j.indcrop.2022.114992"
}

Skorić, M., Ćirić, A., Budimir, S., Janošević, D., Anđelković, B. D., Todosijević, M., Todorović, S., Soković, M., Glamočlija, J., Tešević, V., Gašić, U. M., Mišić, D.,& Kanellis, A. K.. (2022). Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products, 184, 114992.
https://doi.org/10.1016/j.indcrop.2022.114992

Skorić M, Ćirić A, Budimir S, Janošević D, Anđelković BD, Todosijević M, Todorović S, Soković M, Glamočlija J, Tešević V, Gašić UM, Mišić D, Kanellis AK. Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”. in Industrial Crops and Products. 2022;184:114992.
doi:10.1016/j.indcrop.2022.114992 .

Skorić, Marijana, Ćirić, Ana, Budimir, Snežana, Janošević, Dušica, Anđelković, Boban D., Todosijević, Marina, Todorović, Slađana, Soković, Marina, Glamočlija, Jasmina, Tešević, Vele, Gašić, Uroš M., Mišić, Danijela, Kanellis, Angelos K., "Bioactivity-guided identification and isolation of a major antimicrobial compound in Cistus creticus subsp. creticus leaves and resin “ladano”" in Industrial Crops and Products, 184 (2022):114992,
https://doi.org/10.1016/j.indcrop.2022.114992 . .