TY  - JOUR
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1873
AB  - A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - A high-throughput cellulase screening system based on droplet microfluidics
VL  - 8
IS  - 4
DO  - 10.1063/1.4886771
ER  - 

@article{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
abstract = "A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "A high-throughput cellulase screening system based on droplet microfluidics",
volume = "8",
number = "4",
doi = "10.1063/1.4886771"
}

Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics
Amer Inst Physics, Melville., 8(4).
https://doi.org/10.1063/1.4886771

Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. A high-throughput cellulase screening system based on droplet microfluidics. in Biomicrofluidics. 2014;8(4).
doi:10.1063/1.4886771 .

Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "A high-throughput cellulase screening system based on droplet microfluidics" in Biomicrofluidics, 8, no. 4 (2014),
https://doi.org/10.1063/1.4886771 . .

TY  - DATA
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3666
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3666
ER  - 

@misc{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3666"
}

Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R.. (2014). Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771. in Biomicrofluidics
Amer Inst Physics, Melville..
https://hdl.handle.net/21.15107/rcub_cherry_3666

Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771. in Biomicrofluidics. 2014;.
https://hdl.handle.net/21.15107/rcub_cherry_3666 .

Ostafe, Raluca, Prodanović, Radivoje, Ung, Lloyd W., Weitz, David A., Fischer, Rainer, "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771" in Biomicrofluidics (2014),
https://hdl.handle.net/21.15107/rcub_cherry_3666 .

TY  - JOUR
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Commandeur, Ulrich
AU  - Fischer, Rainer
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1606
AB  - We have developed a novel, ultra-high-throughput screening assay for the detection of cellulase activity based on fluorescence-activated cell sorting (FACS) and double emulsion technology. Cellulase activity is detected using a series of coupled enzymes, including hexose oxidase (HOx), which generates hydrogen peroxide from the reducing sugars released by cellulases in the presence of any natural or artificial substrate. The assay can be adapted to suit a microtiter plate format, but the highest throughput is achieved by using FACS to screen high-complexity cellulase clone libraries. Using this approach, we achieved a 12-fold enrichment of positive (cellulase-expressing) cells in cellulase reference libraries after just one sorting round.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Flow cytometry-based ultra-high-throughput screening assay for cellulase activity
VL  - 435
IS  - 1
SP  - 93
EP  - 98
DO  - 10.1016/j.ab.2012.10.043
ER  - 

@article{
author = "Ostafe, Raluca and Prodanović, Radivoje and Commandeur, Ulrich and Fischer, Rainer",
year = "2013",
abstract = "We have developed a novel, ultra-high-throughput screening assay for the detection of cellulase activity based on fluorescence-activated cell sorting (FACS) and double emulsion technology. Cellulase activity is detected using a series of coupled enzymes, including hexose oxidase (HOx), which generates hydrogen peroxide from the reducing sugars released by cellulases in the presence of any natural or artificial substrate. The assay can be adapted to suit a microtiter plate format, but the highest throughput is achieved by using FACS to screen high-complexity cellulase clone libraries. Using this approach, we achieved a 12-fold enrichment of positive (cellulase-expressing) cells in cellulase reference libraries after just one sorting round.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Flow cytometry-based ultra-high-throughput screening assay for cellulase activity",
volume = "435",
number = "1",
pages = "93-98",
doi = "10.1016/j.ab.2012.10.043"
}

Ostafe, R., Prodanović, R., Commandeur, U.,& Fischer, R.. (2013). Flow cytometry-based ultra-high-throughput screening assay for cellulase activity. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 435(1), 93-98.
https://doi.org/10.1016/j.ab.2012.10.043

Ostafe R, Prodanović R, Commandeur U, Fischer R. Flow cytometry-based ultra-high-throughput screening assay for cellulase activity. in Analytical Biochemistry. 2013;435(1):93-98.
doi:10.1016/j.ab.2012.10.043 .

Ostafe, Raluca, Prodanović, Radivoje, Commandeur, Ulrich, Fischer, Rainer, "Flow cytometry-based ultra-high-throughput screening assay for cellulase activity" in Analytical Biochemistry, 435, no. 1 (2013):93-98,
https://doi.org/10.1016/j.ab.2012.10.043 . .

TY  - DATA
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Commandeur, Ulrich
AU  - Fischer, Rainer
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3494
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - Supplementary data for article: Ostafe, R.; Prodanović, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93–98. https://doi.org/10.1016/j.ab.2012.10.043
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3494
ER  - 

@misc{
author = "Ostafe, Raluca and Prodanović, Radivoje and Commandeur, Ulrich and Fischer, Rainer",
year = "2013",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "Supplementary data for article: Ostafe, R.; Prodanović, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93–98. https://doi.org/10.1016/j.ab.2012.10.043",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3494"
}

Ostafe, R., Prodanović, R., Commandeur, U.,& Fischer, R.. (2013). Supplementary data for article: Ostafe, R.; Prodanović, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93–98. https://doi.org/10.1016/j.ab.2012.10.043. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego..
https://hdl.handle.net/21.15107/rcub_cherry_3494

Ostafe R, Prodanović R, Commandeur U, Fischer R. Supplementary data for article: Ostafe, R.; Prodanović, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93–98. https://doi.org/10.1016/j.ab.2012.10.043. in Analytical Biochemistry. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_3494 .

Ostafe, Raluca, Prodanović, Radivoje, Commandeur, Ulrich, Fischer, Rainer, "Supplementary data for article: Ostafe, R.; Prodanović, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93–98. https://doi.org/10.1016/j.ab.2012.10.043" in Analytical Biochemistry (2013),
https://hdl.handle.net/21.15107/rcub_cherry_3494 .