TY  - JOUR
AU  - Jurišević, Milena
AU  - Jagić, Nikola
AU  - Gajović, Nevena
AU  - Arsenijević, Aleksandar
AU  - Jovanović, Milan
AU  - Milovanović, Marija
AU  - Pantić, Jelena
AU  - Jovanović, Ivan
AU  - Sabo, Tibor
AU  - Radosavljević, Gordana
AU  - Arsenijević, Nebojša
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4238
AB  - Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.
PB  - Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer
VL  - 77
IS  - 7
SP  - 715
EP  - 723
DO  - 10.2298/VSP180723149J
ER  - 

@article{
author = "Jurišević, Milena and Jagić, Nikola and Gajović, Nevena and Arsenijević, Aleksandar and Jovanović, Milan and Milovanović, Marija and Pantić, Jelena and Jovanović, Ivan and Sabo, Tibor and Radosavljević, Gordana and Arsenijević, Nebojša",
year = "2020",
abstract = "Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.",
publisher = "Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer",
volume = "77",
number = "7",
pages = "715-723",
doi = "10.2298/VSP180723149J"
}

Jurišević, M., Jagić, N., Gajović, N., Arsenijević, A., Jovanović, M., Milovanović, M., Pantić, J., Jovanović, I., Sabo, T., Radosavljević, G.,& Arsenijević, N.. (2020). O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled
Military Medical Academy, INI., 77(7), 715-723.
https://doi.org/10.2298/VSP180723149J

Jurišević M, Jagić N, Gajović N, Arsenijević A, Jovanović M, Milovanović M, Pantić J, Jovanović I, Sabo T, Radosavljević G, Arsenijević N. O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled. 2020;77(7):715-723.
doi:10.2298/VSP180723149J .

Jurišević, Milena, Jagić, Nikola, Gajović, Nevena, Arsenijević, Aleksandar, Jovanović, Milan, Milovanović, Marija, Pantić, Jelena, Jovanović, Ivan, Sabo, Tibor, Radosavljević, Gordana, Arsenijević, Nebojša, "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer" in Vojnosanitetski pregled, 77, no. 7 (2020):715-723,
https://doi.org/10.2298/VSP180723149J . .

TY  - JOUR
AU  - Đorđević, Dragana S.
AU  - Milovanović, Jelena
AU  - Jurisević, Milena
AU  - Stojanović, Bojana
AU  - Cvetković, Olga
AU  - Pergal, Marija V.
AU  - Ristanović, Elizabeta
AU  - Vojvodić, Danilo
AU  - Simić, Miloš
AU  - Manojlović, Dragan D.
AU  - Milovanović, Marija
AU  - Arsenijević, Nebojša
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3858
AB  - Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays.

The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.
T2  - Serbian Journal of Experimental and Clinical Research
T1  - Antitumour effect of a mixture of n-propyl polysulfides In vitro
VL  - 20
IS  - 4
SP  - 295
EP  - 300
DO  - 10.1515/sjecr-2017-0069
ER  - 

@article{
author = "Đorđević, Dragana S. and Milovanović, Jelena and Jurisević, Milena and Stojanović, Bojana and Cvetković, Olga and Pergal, Marija V. and Ristanović, Elizabeta and Vojvodić, Danilo and Simić, Miloš and Manojlović, Dragan D. and Milovanović, Marija and Arsenijević, Nebojša",
year = "2019",
abstract = "Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays.

The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Antitumour effect of a mixture of n-propyl polysulfides In vitro",
volume = "20",
number = "4",
pages = "295-300",
doi = "10.1515/sjecr-2017-0069"
}

Đorđević, D. S., Milovanović, J., Jurisević, M., Stojanović, B., Cvetković, O., Pergal, M. V., Ristanović, E., Vojvodić, D., Simić, M., Manojlović, D. D., Milovanović, M.,& Arsenijević, N.. (2019). Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research, 20(4), 295-300.
https://doi.org/10.1515/sjecr-2017-0069

Đorđević DS, Milovanović J, Jurisević M, Stojanović B, Cvetković O, Pergal MV, Ristanović E, Vojvodić D, Simić M, Manojlović DD, Milovanović M, Arsenijević N. Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research. 2019;20(4):295-300.
doi:10.1515/sjecr-2017-0069 .

Đorđević, Dragana S., Milovanović, Jelena, Jurisević, Milena, Stojanović, Bojana, Cvetković, Olga, Pergal, Marija V., Ristanović, Elizabeta, Vojvodić, Danilo, Simić, Miloš, Manojlović, Dragan D., Milovanović, Marija, Arsenijević, Nebojša, "Antitumour effect of a mixture of n-propyl polysulfides In vitro" in Serbian Journal of Experimental and Clinical Research, 20, no. 4 (2019):295-300,
https://doi.org/10.1515/sjecr-2017-0069 . .

TY  - JOUR
AU  - Jurisevic, M.
AU  - Arsenijevic, A.
AU  - Pantic, J.
AU  - Gajovic, N.
AU  - Milovanović, Jelena
AU  - Milovanovic, M.
AU  - Poljarević, Jelena
AU  - Sabo, Tibor
AU  - Vojvodic, D.
AU  - Radosavljevic, G.D.
AU  - Arsenijevic, N.
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/335
AB  - Pharmacological treatment of cancer is mostly limited by drug-toxicity and resistance. It has been noticed that new organic ester ligand, O,O'-diethyl-(S,S)- ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (named DEEDCP) showed effective cytotoxic capacities against several human and mouse cancer cell lines. However, its effects on tumor growth and metastasis are unexplored. The aim of present study was to examine the ability of DE-EDCP to inhibit 4T1 murine breast cancer growth and progression and to explore possible molecular mechanisms. DE-EDCP exhibited significant tumoricidal activity on human and murine breast cancer cell lines. Further, marked reduction of murine breast cancer growth and progression by DE-EDCP was shown. DE-EDCP exhibits fewer side-effects compared to cisplatin as a conventional chemotherapeutic. Results obtained from in vivo and in vitro experiments indicate that DE-EDCP induces apoptosis and inhibits proliferation of 4T1 cells. DE-EDCP increases percentage of 4T1 cells in late apoptosis, expression of pro-apoptotic Bax and caspase-3, while decreases expression of anti-apoptotic Bcl-2. DE-EDCP treatment increased the percentage of TUNEL-positive nuclei and reduced Ki-67 expression in breast cancer tissue. DE-EDCP decreased expression of cyclin D3 and Ki-67, increased expression of cyclin-dependent kinase inhibitors p16, p21 and p27 and arrested 4T1 cells in G0/G1 cell cycle phase. Expression of STAT3 and downstream regulated molecules, NANOG and SOX2, was reduced in 4T1 cells after DE-EDCP treatment. In conclusion, DE-EDCP impairs breast cancer growth and progression by triggering cancer cell death and inhibition of cancer cell proliferation. DE-EDCP might be of interest in the development of the new anticancer agent. ©Jurisevic et al.
T2  - Oncotarget
T1  - The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis
VL  - 9
IS  - 46
SP  - 28195
EP  - 28212
DO  - 10.18632/oncotarget.25610
ER  - 

@article{
author = "Jurisevic, M. and Arsenijevic, A. and Pantic, J. and Gajovic, N. and Milovanović, Jelena and Milovanovic, M. and Poljarević, Jelena and Sabo, Tibor and Vojvodic, D. and Radosavljevic, G.D. and Arsenijevic, N.",
year = "2018",
abstract = "Pharmacological treatment of cancer is mostly limited by drug-toxicity and resistance. It has been noticed that new organic ester ligand, O,O'-diethyl-(S,S)- ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (named DEEDCP) showed effective cytotoxic capacities against several human and mouse cancer cell lines. However, its effects on tumor growth and metastasis are unexplored. The aim of present study was to examine the ability of DE-EDCP to inhibit 4T1 murine breast cancer growth and progression and to explore possible molecular mechanisms. DE-EDCP exhibited significant tumoricidal activity on human and murine breast cancer cell lines. Further, marked reduction of murine breast cancer growth and progression by DE-EDCP was shown. DE-EDCP exhibits fewer side-effects compared to cisplatin as a conventional chemotherapeutic. Results obtained from in vivo and in vitro experiments indicate that DE-EDCP induces apoptosis and inhibits proliferation of 4T1 cells. DE-EDCP increases percentage of 4T1 cells in late apoptosis, expression of pro-apoptotic Bax and caspase-3, while decreases expression of anti-apoptotic Bcl-2. DE-EDCP treatment increased the percentage of TUNEL-positive nuclei and reduced Ki-67 expression in breast cancer tissue. DE-EDCP decreased expression of cyclin D3 and Ki-67, increased expression of cyclin-dependent kinase inhibitors p16, p21 and p27 and arrested 4T1 cells in G0/G1 cell cycle phase. Expression of STAT3 and downstream regulated molecules, NANOG and SOX2, was reduced in 4T1 cells after DE-EDCP treatment. In conclusion, DE-EDCP impairs breast cancer growth and progression by triggering cancer cell death and inhibition of cancer cell proliferation. DE-EDCP might be of interest in the development of the new anticancer agent. ©Jurisevic et al.",
journal = "Oncotarget",
title = "The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis",
volume = "9",
number = "46",
pages = "28195-28212",
doi = "10.18632/oncotarget.25610"
}

Jurisevic, M., Arsenijevic, A., Pantic, J., Gajovic, N., Milovanović, J., Milovanovic, M., Poljarević, J., Sabo, T., Vojvodic, D., Radosavljevic, G.D.,& Arsenijevic, N.. (2018). The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis. in Oncotarget, 9(46), 28195-28212.
https://doi.org/10.18632/oncotarget.25610

Jurisevic M, Arsenijevic A, Pantic J, Gajovic N, Milovanović J, Milovanovic M, Poljarević J, Sabo T, Vojvodic D, Radosavljevic G, Arsenijevic N. The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis. in Oncotarget. 2018;9(46):28195-28212.
doi:10.18632/oncotarget.25610 .

Jurisevic, M., Arsenijevic, A., Pantic, J., Gajovic, N., Milovanović, Jelena, Milovanovic, M., Poljarević, Jelena, Sabo, Tibor, Vojvodic, D., Radosavljevic, G.D., Arsenijevic, N., "The organic ester O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride attenuates murine breast cancer growth and metastasis" in Oncotarget, 9, no. 46 (2018):28195-28212,
https://doi.org/10.18632/oncotarget.25610 . .

TY  - JOUR
AU  - Jurišević, Milena
AU  - Radosavljević, Gordana
AU  - Arsenijević, Aleksandar
AU  - Milovanović, Marija
AU  - Gajović, Nevena
AU  - Đorđević, Dragana S.
AU  - Milovanović, Jelena
AU  - Stojanović, Bojana
AU  - Ilić, Aleksandar
AU  - Sabo, Tibor
AU  - Kanjevac, Tatjana
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/216
AB  - e design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. is article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known to have a high level of cisplatin-resistance. Exanimate complexes appear to have a diff erent mode of mechanism of action compared with cisplatin which includes apoptotic and necrotic cell death. ere are indications that further investigations of these compounds may be very useful in overcoming the problems associated global cancer statistic. © 2016, University of Kragujevac, Faculty of Science. All rights reserved.
AB  - Kompleski platine koriste se kao osnova za dizajn novih lekova. Oni su u širokoj upotrebi kao antitumorski agensi i do danas je oko 30 komplesa platine(II) i platine(IV) u nekoj od faza kliničkog ispitivanja. Posebno mesto u današnjim istaživanjima zauzimaju kompleksi metala sa edda ligandima. Uspešno je sintetisan veliki broj novih edda liganda i odgovarajućih kompleksa. Neki od ovih agensa pokazuju bolju aktivnost od zlatnog standarda, cisplatine. Pokazano je da postoji moguća veza između dužine ugljovodiničnog lanca estraske grupe liganda i citotoksičnog efekta. U većini slučajeva dužina lanca direktno korelira sa antitumorskom aktivnošću. Zabeležena je efikasnija citotoksicna aktivnost određenih kompleska platine sa edda ligandima na ćelijskim linijama tumora koji pokazuju odgovarajući stepen rezistencije na cisplatinu. Ispitivani komleksi imaju različit mehanizam dejstva od cisplatine, koji uključuje elemente nekrotične i programirane ćelijske smrti. Postoje nagoveštaji da dalja istraživanja ovih agensa mogu biti značajna za prevazilaženje globalnog problema sa kojim se svet danas suočava, a koji se odnosi na stalni porast osoba obolelih od karcinoma.
T2  - Serbian Journal of Experimental and Clinical Research (ranije: Medicus)
T1  - Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]
T1  - Kompleksi platine sa edda (etilendiamin-N, N'-diacetat) ligandima kao potencijalni antitumorski agensi
VL  - 17
IS  - 4
SP  - 285
EP  - 295
DO  - 10.1515/SJECR-2016-0042
ER  - 

@article{
author = "Jurišević, Milena and Radosavljević, Gordana and Arsenijević, Aleksandar and Milovanović, Marija and Gajović, Nevena and Đorđević, Dragana S. and Milovanović, Jelena and Stojanović, Bojana and Ilić, Aleksandar and Sabo, Tibor and Kanjevac, Tatjana",
year = "2016",
abstract = "e design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. is article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known to have a high level of cisplatin-resistance. Exanimate complexes appear to have a diff erent mode of mechanism of action compared with cisplatin which includes apoptotic and necrotic cell death. ere are indications that further investigations of these compounds may be very useful in overcoming the problems associated global cancer statistic. © 2016, University of Kragujevac, Faculty of Science. All rights reserved., Kompleski platine koriste se kao osnova za dizajn novih lekova. Oni su u širokoj upotrebi kao antitumorski agensi i do danas je oko 30 komplesa platine(II) i platine(IV) u nekoj od faza kliničkog ispitivanja. Posebno mesto u današnjim istaživanjima zauzimaju kompleksi metala sa edda ligandima. Uspešno je sintetisan veliki broj novih edda liganda i odgovarajućih kompleksa. Neki od ovih agensa pokazuju bolju aktivnost od zlatnog standarda, cisplatine. Pokazano je da postoji moguća veza između dužine ugljovodiničnog lanca estraske grupe liganda i citotoksičnog efekta. U većini slučajeva dužina lanca direktno korelira sa antitumorskom aktivnošću. Zabeležena je efikasnija citotoksicna aktivnost određenih kompleska platine sa edda ligandima na ćelijskim linijama tumora koji pokazuju odgovarajući stepen rezistencije na cisplatinu. Ispitivani komleksi imaju različit mehanizam dejstva od cisplatine, koji uključuje elemente nekrotične i programirane ćelijske smrti. Postoje nagoveštaji da dalja istraživanja ovih agensa mogu biti značajna za prevazilaženje globalnog problema sa kojim se svet danas suočava, a koji se odnosi na stalni porast osoba obolelih od karcinoma.",
journal = "Serbian Journal of Experimental and Clinical Research (ranije: Medicus)",
title = "Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi], Kompleksi platine sa edda (etilendiamin-N, N'-diacetat) ligandima kao potencijalni antitumorski agensi",
volume = "17",
number = "4",
pages = "285-295",
doi = "10.1515/SJECR-2016-0042"
}

Jurišević, M., Radosavljević, G., Arsenijević, A., Milovanović, M., Gajović, N., Đorđević, D. S., Milovanović, J., Stojanović, B., Ilić, A., Sabo, T.,& Kanjevac, T.. (2016). Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]. in Serbian Journal of Experimental and Clinical Research (ranije: Medicus), 17(4), 285-295.
https://doi.org/10.1515/SJECR-2016-0042

Jurišević M, Radosavljević G, Arsenijević A, Milovanović M, Gajović N, Đorđević DS, Milovanović J, Stojanović B, Ilić A, Sabo T, Kanjevac T. Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]. in Serbian Journal of Experimental and Clinical Research (ranije: Medicus). 2016;17(4):285-295.
doi:10.1515/SJECR-2016-0042 .

Jurišević, Milena, Radosavljević, Gordana, Arsenijević, Aleksandar, Milovanović, Marija, Gajović, Nevena, Đorđević, Dragana S., Milovanović, Jelena, Stojanović, Bojana, Ilić, Aleksandar, Sabo, Tibor, Kanjevac, Tatjana, "Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]" in Serbian Journal of Experimental and Clinical Research (ranije: Medicus), 17, no. 4 (2016):285-295,
https://doi.org/10.1515/SJECR-2016-0042 . .

TY  - JOUR
AU  - Kanjevac, Tatjana V.
AU  - Milovanović, Marija
AU  - Milosevic-Djordjevic, Olivera
AU  - Tešić, Živoslav Lj.
AU  - Ivanovic, Mirjana
AU  - Lukic, Aleksandra
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1740
AB  - Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations
VL  - 67
IS  - 2
SP  - 619
EP  - 630
DO  - 10.2298/ABS141021022K
ER  - 

@article{
author = "Kanjevac, Tatjana V. and Milovanović, Marija and Milosevic-Djordjevic, Olivera and Tešić, Živoslav Lj. and Ivanovic, Mirjana and Lukic, Aleksandra",
year = "2015",
abstract = "Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations",
volume = "67",
number = "2",
pages = "619-630",
doi = "10.2298/ABS141021022K"
}

Kanjevac, T. V., Milovanović, M., Milosevic-Djordjevic, O., Tešić, Ž. Lj., Ivanovic, M.,& Lukic, A.. (2015). Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(2), 619-630.
https://doi.org/10.2298/ABS141021022K

Kanjevac TV, Milovanović M, Milosevic-Djordjevic O, Tešić ŽL, Ivanovic M, Lukic A. Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations. in Archives of biological sciences. 2015;67(2):619-630.
doi:10.2298/ABS141021022K .

Kanjevac, Tatjana V., Milovanović, Marija, Milosevic-Djordjevic, Olivera, Tešić, Živoslav Lj., Ivanovic, Mirjana, Lukic, Aleksandra, "Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations" in Archives of biological sciences, 67, no. 2 (2015):619-630,
https://doi.org/10.2298/ABS141021022K . .