Trophoblast and extraembryonic fetal cells: plasticity, differentiation factors and in vitro modulation of functional characteristics

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Trophoblast and extraembryonic fetal cells: plasticity, differentiation factors and in vitro modulation of functional characteristics (en)
Трофобласт и екстраембрионалне феталне ћелије: пластичност, фактори диференцијације и ин витро модулација функционалних својстава (sr)
Trofoblast i ekstraembrionalne fetalne ćelije: plastičnost, faktori diferencijacije i in vitro modulacija funkcionalnih svojstava (sr_RS)
Authors

Publications

Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity

Stanković, Nada; Šenerović, Lidija; Bojić-Trbojević, Žanka; Vučković, Ivan; Vicovac, Ljiljana; Vasiljević, Branka; Nikodinović-Runić, Jasmina

(Wiley, Hoboken, 2013) 

TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Žanka
AU  - Vučković, Ivan
AU  - Vicovac, Ljiljana
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1438
AB  - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
VL  - 115
IS  - 6
SP  - 1297
EP  - 1306
DO  - 10.1111/jam.12326
ER  - 
@article{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Žanka and Vučković, Ivan and Vicovac, Ljiljana and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity",
volume = "115",
number = "6",
pages = "1297-1306",
doi = "10.1111/jam.12326"
}
Stanković, N., Šenerović, L., Bojić-Trbojević, Ž., Vučković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology
Wiley, Hoboken., 115(6), 1297-1306.
https://doi.org/10.1111/jam.12326
Stanković N, Šenerović L, Bojić-Trbojević Ž, Vučković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306.
doi:10.1111/jam.12326 .
Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Žanka, Vučković, Ivan, Vicovac, Ljiljana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306,
https://doi.org/10.1111/jam.12326 . .
3
9
8
9
9

Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity

Stanković, Nada; Šenerović, Lidija; Bojić-Trbojević, Žanka; Vučković, Ivan; Vicovac, Ljiljana; Vasiljević, Branka; Nikodinović-Runić, Jasmina

(Wiley, Hoboken, 2013) 

TY  - JOUR
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Žanka
AU  - Vučković, Ivan
AU  - Vicovac, Ljiljana
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3486
AB  - AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity
VL  - 115
IS  - 6
SP  - 1297
EP  - 1306
DO  - 10.1111/jam.12326
ER  - 
@article{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Žanka and Vučković, Ivan and Vicovac, Ljiljana and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "AimsThe aim of this study was to improve production of pentaene 32,33-didehydroroflamycoin (DDHR) in Streptomyces durmitorensis MS405 strain to obtain quantities sufficient for in depth analysis of antimicrobial properties. Methods and ResultsThrough classical medium optimization conditions for stable growth, DDHR production within 7days of incubation was established. Yields of 215mgl(-1) were achieved in shake flask experiments in complex medium with mannitol as the primary carbon source. DDHR had poor antibacterial activity with minimal inhibitory concentrations (MIC) of 400gml(-1) for Staphylococcus aureus and Bacillus subtilis, while MIC of 70gml(-1) was determined for Candida albicans. Using flow cytometry and fluorescent microscopy, it was demonstrated that DDHR induced membrane damage in C.albicans followed by cell death. Combination studies with known antifungal nystatin showed that DDHR is a promising agent for the development of novel antimycotic treatments potentially less toxic for human cells. ConclusionsPentaene didehydroroflamycoin has no antibacterial activity but can be further developed for the application in antifungal therapy. Significance and Impact of the StudyThis study is the first report on the stable and production in high yields of a novel pentaene family that acts on Candida cell membranes and can be used in combination with known antifungals. Polyenes are still antifungal antibiotics of choice, and therefore, isolation and production of new lead structures are highly significant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity",
volume = "115",
number = "6",
pages = "1297-1306",
doi = "10.1111/jam.12326"
}
Stanković, N., Šenerović, L., Bojić-Trbojević, Ž., Vučković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology
Wiley, Hoboken., 115(6), 1297-1306.
https://doi.org/10.1111/jam.12326
Stanković N, Šenerović L, Bojić-Trbojević Ž, Vučković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity. in Journal of Applied Microbiology. 2013;115(6):1297-1306.
doi:10.1111/jam.12326 .
Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Žanka, Vučković, Ivan, Vicovac, Ljiljana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Didehydroroflamycoin pentaene macrolide family from Streptomyces durmitorensis MS405(T): production optimization and antimicrobial activity" in Journal of Applied Microbiology, 115, no. 6 (2013):1297-1306,
https://doi.org/10.1111/jam.12326 . .
3
9
8
9
9

Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326

Stanković, Nada; Šenerović, Lidija; Bojić-Trbojević, Žanka; Vučković, Ivan; Vicovac, Ljiljana; Vasiljević, Branka; Nikodinović-Runić, Jasmina

(Wiley, Hoboken, 2013) 

TY  - DATA
AU  - Stanković, Nada
AU  - Šenerović, Lidija
AU  - Bojić-Trbojević, Žanka
AU  - Vučković, Ivan
AU  - Vicovac, Ljiljana
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3487
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3487
ER  - 
@misc{
author = "Stanković, Nada and Šenerović, Lidija and Bojić-Trbojević, Žanka and Vučković, Ivan and Vicovac, Ljiljana and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2013",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3487"
}
Stanković, N., Šenerović, L., Bojić-Trbojević, Ž., Vučković, I., Vicovac, L., Vasiljević, B.,& Nikodinović-Runić, J.. (2013). Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326. in Journal of Applied Microbiology
Wiley, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3487
Stanković N, Šenerović L, Bojić-Trbojević Ž, Vučković I, Vicovac L, Vasiljević B, Nikodinović-Runić J. Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326. in Journal of Applied Microbiology. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_3487 .
Stanković, Nada, Šenerović, Lidija, Bojić-Trbojević, Žanka, Vučković, Ivan, Vicovac, Ljiljana, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Supplementary data for article: Stanković, N.; Šenerović, L.; Bojic-Trbojevic, Z.; Vuckovic, I.; Vicovac, L.; Vasiljevic, B.; Nikodinović-Runić, J. Didehydroroflamycoin Pentaene Macrolide Family from Streptomyces Durmitorensis MS405(T): Production Optimization and Antimicrobial Activity. Journal of Applied Microbiology 2013, 115 (6), 1297–1306. https://doi.org/10.1111/jam.12326" in Journal of Applied Microbiology (2013),
https://hdl.handle.net/21.15107/rcub_cherry_3487 .