TY  - JOUR
AU  - Bonnin, Estelle
AU  - Mutić, Jelena
AU  - Nikolić, Jasna
AU  - Burr, Sally
AU  - Robert, Paul
AU  - Crepeau, Marie-Jeanne
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1697
AB  - Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls
VL  - 124
SP  - 57
EP  - 65
DO  - 10.1016/j.carbpol.2015.01.074
ER  - 

@article{
author = "Bonnin, Estelle and Mutić, Jelena and Nikolić, Jasna and Burr, Sally and Robert, Paul and Crepeau, Marie-Jeanne",
year = "2015",
abstract = "Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls",
volume = "124",
pages = "57-65",
doi = "10.1016/j.carbpol.2015.01.074"
}

Bonnin, E., Mutić, J., Nikolić, J., Burr, S., Robert, P.,& Crepeau, M.. (2015). Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 124, 57-65.
https://doi.org/10.1016/j.carbpol.2015.01.074

Bonnin E, Mutić J, Nikolić J, Burr S, Robert P, Crepeau M. Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers. 2015;124:57-65.
doi:10.1016/j.carbpol.2015.01.074 .

Bonnin, Estelle, Mutić, Jelena, Nikolić, Jasna, Burr, Sally, Robert, Paul, Crepeau, Marie-Jeanne, "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls" in Carbohydrate Polymers, 124 (2015):57-65,
https://doi.org/10.1016/j.carbpol.2015.01.074 . .