Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200288 (Inovacioni centar Hemijskog fakulteta u Beogradu doo) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200288 (Иновациони центар Хемијског факултета у Београду доо) (sr)
Authors

Publications

Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis

Veličković, Luka; Nikolić, Milan; Minić, Simeon

(Belgrade : Serbian Biochemical Society, 2022)

TY  - CONF
AU  - Veličković, Luka
AU  - Nikolić, Milan
AU  - Minić, Simeon
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5780
AB  - C-phycocyanin (C-PC), the major protein of cyanobacteria Arthrospira platensis, is a blue
pigment that primarily transfers energy during photosynthesis.It has diverse
biotechnological applications since it can be used in nutraceutical, cosmetics,
pharmaceutical industries, and biomedical research.Its intensive blue colour and strong
antioxidant activity give C-PC significant potential to replace synthetic colourants in the
food industry. However, thermal treatment of food has detrimental effects on C-PC colour
due to sensitivity to higher temperatures; therefore,the application of this natural colourant
in food and other products is limited. Hence, improving C-PC stability is the major
challenge for successful application in food and beverages colouring. In the light of this,we
aim to investigate the thermal stability of C-PC in the presence of selected sugars (glucose,
fructose and sucrose), commonly used in the food industry. Ex-situabsorption
spectrophotometryshowed that 18% solution (w/v) of glucose, sucrose and fructose at pH
7,upon incubation at 65℃, exhibit 91.4, 52.9 and 52.5% of colour preservation,
respectively.In situ fluorescence measurements revealed that free C-PC has a melting point
of 55.4°C, while the presence of glucose and sucrose increases the melting point of C-PC
to 64.4 and 61.4°C, respectively. On the other hand, fructose does not significantly
influence the C-PC melting point. These results show that the thermal stability ofthe CPCsolution is substantially increased in the presence of sugars, while the type of sugar
significantly determines the extent of the stabilisation effect. Overall, our study provides
the strategy for enhancing the application potential of C-PC as natural food colourant,
providing a food product with vivid blue colour and substantial antioxidant activities.
PB  - Belgrade : Serbian Biochemical Society
C3  - Serbian Biochemical Society, The XI Conference "Amazing Biochemistry"  Novi Sad, Faculty of Sciences, 22nd and 23rd of September, 2022
T1  - Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis
SP  - 165
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5780
ER  - 
@conference{
author = "Veličković, Luka and Nikolić, Milan and Minić, Simeon",
year = "2022",
abstract = "C-phycocyanin (C-PC), the major protein of cyanobacteria Arthrospira platensis, is a blue
pigment that primarily transfers energy during photosynthesis.It has diverse
biotechnological applications since it can be used in nutraceutical, cosmetics,
pharmaceutical industries, and biomedical research.Its intensive blue colour and strong
antioxidant activity give C-PC significant potential to replace synthetic colourants in the
food industry. However, thermal treatment of food has detrimental effects on C-PC colour
due to sensitivity to higher temperatures; therefore,the application of this natural colourant
in food and other products is limited. Hence, improving C-PC stability is the major
challenge for successful application in food and beverages colouring. In the light of this,we
aim to investigate the thermal stability of C-PC in the presence of selected sugars (glucose,
fructose and sucrose), commonly used in the food industry. Ex-situabsorption
spectrophotometryshowed that 18% solution (w/v) of glucose, sucrose and fructose at pH
7,upon incubation at 65℃, exhibit 91.4, 52.9 and 52.5% of colour preservation,
respectively.In situ fluorescence measurements revealed that free C-PC has a melting point
of 55.4°C, while the presence of glucose and sucrose increases the melting point of C-PC
to 64.4 and 61.4°C, respectively. On the other hand, fructose does not significantly
influence the C-PC melting point. These results show that the thermal stability ofthe CPCsolution is substantially increased in the presence of sugars, while the type of sugar
significantly determines the extent of the stabilisation effect. Overall, our study provides
the strategy for enhancing the application potential of C-PC as natural food colourant,
providing a food product with vivid blue colour and substantial antioxidant activities.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Serbian Biochemical Society, The XI Conference "Amazing Biochemistry"  Novi Sad, Faculty of Sciences, 22nd and 23rd of September, 2022",
title = "Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis",
pages = "165-165",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5780"
}
Veličković, L., Nikolić, M.,& Minić, S.. (2022). Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis. in Serbian Biochemical Society, The XI Conference "Amazing Biochemistry"  Novi Sad, Faculty of Sciences, 22nd and 23rd of September, 2022
Belgrade : Serbian Biochemical Society., 165-165.
https://hdl.handle.net/21.15107/rcub_cherry_5780
Veličković L, Nikolić M, Minić S. Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis. in Serbian Biochemical Society, The XI Conference "Amazing Biochemistry"  Novi Sad, Faculty of Sciences, 22nd and 23rd of September, 2022. 2022;:165-165.
https://hdl.handle.net/21.15107/rcub_cherry_5780 .
Veličković, Luka, Nikolić, Milan, Minić, Simeon, "Sugar-mediated thermal stabilisation of C-phycocyanin from Arthrospira platensis" in Serbian Biochemical Society, The XI Conference "Amazing Biochemistry"  Novi Sad, Faculty of Sciences, 22nd and 23rd of September, 2022 (2022):165-165,
https://hdl.handle.net/21.15107/rcub_cherry_5780 .

Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity

Mirković, Marija D.; Radović, Magdalena; Stanković, Dalibor; Vranješ-Đurić, Sanja; Janković, Drina; Petrović, Djordje; Mihajlović-Lalić, Ljiljana E.; Prijović, Željko; Milanović, Zorana

(Taylor & Francis Ltd, 2022)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Stanković, Dalibor
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Petrović, Djordje
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Prijović, Željko
AU  - Milanović, Zorana
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5135
AB  - Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog, ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1-3, were further investigated in terms of their electrochemical behavior. The binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interaction. The binding reaction with HSA showed for 1 and 3 complexes decrease in the peak current obtained in the case of complexes before the addition of HSA, while resulted compound obtained from Ni complex – HSA possesses the same electroactivity as starting complex. Furthermore, the cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.
PB  - Taylor & Francis Ltd
T2  - Journal of Coordination Chemistry
T1  - Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity
VL  - 75
IS  - 1-2
DO  - 10.1080/00958972.2022.2032683
ER  - 
@article{
author = "Mirković, Marija D. and Radović, Magdalena and Stanković, Dalibor and Vranješ-Đurić, Sanja and Janković, Drina and Petrović, Djordje and Mihajlović-Lalić, Ljiljana E. and Prijović, Željko and Milanović, Zorana",
year = "2022",
abstract = "Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog, ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1-3, were further investigated in terms of their electrochemical behavior. The binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interaction. The binding reaction with HSA showed for 1 and 3 complexes decrease in the peak current obtained in the case of complexes before the addition of HSA, while resulted compound obtained from Ni complex – HSA possesses the same electroactivity as starting complex. Furthermore, the cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.",
publisher = "Taylor & Francis Ltd",
journal = "Journal of Coordination Chemistry",
title = "Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity",
volume = "75",
number = "1-2",
doi = "10.1080/00958972.2022.2032683"
}
Mirković, M. D., Radović, M., Stanković, D., Vranješ-Đurić, S., Janković, D., Petrović, D., Mihajlović-Lalić, L. E., Prijović, Ž.,& Milanović, Z.. (2022). Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity. in Journal of Coordination Chemistry
Taylor & Francis Ltd., 75(1-2).
https://doi.org/10.1080/00958972.2022.2032683
Mirković MD, Radović M, Stanković D, Vranješ-Đurić S, Janković D, Petrović D, Mihajlović-Lalić LE, Prijović Ž, Milanović Z. Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity. in Journal of Coordination Chemistry. 2022;75(1-2).
doi:10.1080/00958972.2022.2032683 .
Mirković, Marija D., Radović, Magdalena, Stanković, Dalibor, Vranješ-Đurić, Sanja, Janković, Drina, Petrović, Djordje, Mihajlović-Lalić, Ljiljana E., Prijović, Željko, Milanović, Zorana, "Co(III), Ni(II), and Cu(II) complexes with tetradentate Schiff base ligand: Synthesis, Characterization, Electrochemical Behavior, Binding assessment and In vitro cytotoxicity activity" in Journal of Coordination Chemistry, 75, no. 1-2 (2022),
https://doi.org/10.1080/00958972.2022.2032683 . .
1
1

Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients

Stojsavljević, Aleksandar; Ristić‑Medić, Danijela; Krstić, Đurđa D.; Rovčanin, Branislav; Radjen, Slavica; Terzić, Brankica; Manojlović, Dragan D.

(Springer, 2022)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Ristić‑Medić, Danijela
AU  - Krstić, Đurđa D.
AU  - Rovčanin, Branislav
AU  - Radjen, Slavica
AU  - Terzić, Brankica
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5195
AB  - The status of essential and toxic trace elements in patients with different stages of chronic kidney disease (CKD) is still
unclear and not well characterized. The present study examined the circulatory levels of a wide panel of trace elements (Al,
Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Cd, Pb, and U) in hemodialysis patients (HD group) and pre-dialysis patients with
stage 3 CKD (PD group). Comparisons were made between groups of patients and healthy individuals from the control group
(CG). The levels of Al, Mn, Co, Ni, Cu, As, Se, Sr, and Pb were higher, while the levels of Cr, Zn, Rb, Cd, and U were lower
in HD patients than in our CG. Higher levels of Al and Se, as well as lower levels of As, Sr, Zn, Rb, and U were significant
and distinguished HD from PD. Among other analyzed elements, Co, Se, and U are the only trace elements that did not
distinguish PD from CG at a statistically significant level. The HD group had lower serum U levels than the PD group, and
this could be a result of hemodialysis. This study also revealed that the Cu/Zn ratio could be used as a marker for early and
late detection of renal failure. Marked changes of essential and toxic trace element levels in sera indicate additional pathophysiological
events in CKD, which could additionally contribute to the preexisting increased morbidity of HD patients.
Measurement of trace elements in HD patients should be performed routinely.
PB  - Springer
T2  - Biological Trace Element Research
T1  - Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients
VL  - 200
SP  - 3117
EP  - 3125
DO  - 10.1007/s12011-021-02940-7
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Ristić‑Medić, Danijela and Krstić, Đurđa D. and Rovčanin, Branislav and Radjen, Slavica and Terzić, Brankica and Manojlović, Dragan D.",
year = "2022",
abstract = "The status of essential and toxic trace elements in patients with different stages of chronic kidney disease (CKD) is still
unclear and not well characterized. The present study examined the circulatory levels of a wide panel of trace elements (Al,
Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Cd, Pb, and U) in hemodialysis patients (HD group) and pre-dialysis patients with
stage 3 CKD (PD group). Comparisons were made between groups of patients and healthy individuals from the control group
(CG). The levels of Al, Mn, Co, Ni, Cu, As, Se, Sr, and Pb were higher, while the levels of Cr, Zn, Rb, Cd, and U were lower
in HD patients than in our CG. Higher levels of Al and Se, as well as lower levels of As, Sr, Zn, Rb, and U were significant
and distinguished HD from PD. Among other analyzed elements, Co, Se, and U are the only trace elements that did not
distinguish PD from CG at a statistically significant level. The HD group had lower serum U levels than the PD group, and
this could be a result of hemodialysis. This study also revealed that the Cu/Zn ratio could be used as a marker for early and
late detection of renal failure. Marked changes of essential and toxic trace element levels in sera indicate additional pathophysiological
events in CKD, which could additionally contribute to the preexisting increased morbidity of HD patients.
Measurement of trace elements in HD patients should be performed routinely.",
publisher = "Springer",
journal = "Biological Trace Element Research",
title = "Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients",
volume = "200",
pages = "3117-3125",
doi = "10.1007/s12011-021-02940-7"
}
Stojsavljević, A., Ristić‑Medić, D., Krstić, Đ. D., Rovčanin, B., Radjen, S., Terzić, B.,& Manojlović, D. D.. (2022). Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients. in Biological Trace Element Research
Springer., 200, 3117-3125.
https://doi.org/10.1007/s12011-021-02940-7
Stojsavljević A, Ristić‑Medić D, Krstić ĐD, Rovčanin B, Radjen S, Terzić B, Manojlović DD. Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients. in Biological Trace Element Research. 2022;200:3117-3125.
doi:10.1007/s12011-021-02940-7 .
Stojsavljević, Aleksandar, Ristić‑Medić, Danijela, Krstić, Đurđa D., Rovčanin, Branislav, Radjen, Slavica, Terzić, Brankica, Manojlović, Dragan D., "Circulatory Imbalance of Essential and Toxic Trace Elements in Pre‑dialysis and Hemodialysis Patients" in Biological Trace Element Research, 200 (2022):3117-3125,
https://doi.org/10.1007/s12011-021-02940-7 . .
1

Levels of non-essential trace metals and their impact on placental health: a review

Stojsavljević, Aleksandar; Perović, Milan; Nešić, Andrijana N.; Miković, Željko; Manojlović, Dragan D.

(SpringerLink, 2022)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Perović, Milan
AU  - Nešić, Andrijana N.
AU  - Miković, Željko
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - https://link.springer.com/article/10.1007/s11356-022-20205-1
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5116
AB  - According to recent research, even low levels of environmental chemicals, particularly heavy metals, can considerably disrupt placental homeostasis. This review aims to explore the profile of non-essential trace metals in placental tissues across the globe and to specify trace metal(s) that can be candidates for impaired placental health. Accordingly, we conducted an extensive survey on relevant databases of peer-reviewed papers published in the last two decades. Among a considerable number of non-essential trace metals, arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg) were identified as the most detrimental to placental health. Comparative analysis showed remarkable differences in placental levels of these trace metals worldwide. Based on current data reported across the globe, a median (min–max) range from 0.55 to 15 ng/g for placental As levels could be deemed safe. The placental Cd and Pb levels were markedly higher in smokers than in non-smokers. Occupationally exposed pregnant women had several orders of magnitude higher Cd, Pb, and Hg levels in placental tissues than non-occupationally exposed women. Also, we concluded that even low-level exposure to As, Cd, Pb, and Hg could be deleterious to proper fetal development. This review implies the need to reduce exposure to non-essential trace metals to preserve placental health and prevent numerous poor pregnancy outcomes. Overall, the information presented is expected to help plan future fundamental and applied investigations on the placental toxicity of As, Cd, Pb, and Hg. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PB  - SpringerLink
T2  - Environmental Science and Pollution Research
T1  - Levels of non-essential trace metals and their impact on placental health: a review
VL  - 29
SP  - 43662
EP  - 43674
DO  - 10.1007/s11356-022-20205-1
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Perović, Milan and Nešić, Andrijana N. and Miković, Željko and Manojlović, Dragan D.",
year = "2022",
abstract = "According to recent research, even low levels of environmental chemicals, particularly heavy metals, can considerably disrupt placental homeostasis. This review aims to explore the profile of non-essential trace metals in placental tissues across the globe and to specify trace metal(s) that can be candidates for impaired placental health. Accordingly, we conducted an extensive survey on relevant databases of peer-reviewed papers published in the last two decades. Among a considerable number of non-essential trace metals, arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg) were identified as the most detrimental to placental health. Comparative analysis showed remarkable differences in placental levels of these trace metals worldwide. Based on current data reported across the globe, a median (min–max) range from 0.55 to 15 ng/g for placental As levels could be deemed safe. The placental Cd and Pb levels were markedly higher in smokers than in non-smokers. Occupationally exposed pregnant women had several orders of magnitude higher Cd, Pb, and Hg levels in placental tissues than non-occupationally exposed women. Also, we concluded that even low-level exposure to As, Cd, Pb, and Hg could be deleterious to proper fetal development. This review implies the need to reduce exposure to non-essential trace metals to preserve placental health and prevent numerous poor pregnancy outcomes. Overall, the information presented is expected to help plan future fundamental and applied investigations on the placental toxicity of As, Cd, Pb, and Hg. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",
publisher = "SpringerLink",
journal = "Environmental Science and Pollution Research",
title = "Levels of non-essential trace metals and their impact on placental health: a review",
volume = "29",
pages = "43662-43674",
doi = "10.1007/s11356-022-20205-1"
}
Stojsavljević, A., Perović, M., Nešić, A. N., Miković, Ž.,& Manojlović, D. D.. (2022). Levels of non-essential trace metals and their impact on placental health: a review. in Environmental Science and Pollution Research
SpringerLink., 29, 43662-43674.
https://doi.org/10.1007/s11356-022-20205-1
Stojsavljević A, Perović M, Nešić AN, Miković Ž, Manojlović DD. Levels of non-essential trace metals and their impact on placental health: a review. in Environmental Science and Pollution Research. 2022;29:43662-43674.
doi:10.1007/s11356-022-20205-1 .
Stojsavljević, Aleksandar, Perović, Milan, Nešić, Andrijana N., Miković, Željko, Manojlović, Dragan D., "Levels of non-essential trace metals and their impact on placental health: a review" in Environmental Science and Pollution Research, 29 (2022):43662-43674,
https://doi.org/10.1007/s11356-022-20205-1 . .
3
3

Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood

Stojsavljević, Aleksandar; Rovčanin, Marija; Jagodić, Jovana; Miković, Željko; Jeremić, Ana; Perović, Milan; Manojlović, Dragan D.

(SpringerLink, 2022)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Marija
AU  - Jagodić, Jovana
AU  - Miković, Željko
AU  - Jeremić, Ana
AU  - Perović, Milan
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5320
AB  - Due to the recognized harmful effects of some trace elements on fetal development after long-term exposures, it is first important to address their basic/physiological levels before monitoring toxicological effects and clinical outcomes on prenatal and postnatal health. This research aimed to define, for the first time, reference values for multiple (ultra) trace elements in umbilical cord blood (UCB) plasma samples of a notable number of healthy pregnant women (n = 125). All samples were collected during 2020–2021 and all participants were from Belgrade and two regions (Šumadija and Podunavlje). Following trace elements were enrolled in this study: essential (Mn, Co, Cu, Zn, Se, Mo), toxic (Be, Al, Ni, As, Rb, Sr, Ag, Cd, Sb, Ba, Tl, Pb, Th, U), rare earth (La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm), and noble metals (Ru, Rh, Pd, Re, Ir, Os, Pt). UCB plasma samples were diluted with suitable solvents and analyzed by inductively coupled plasma-mass spectrometry. The analytical technique used was validated by the standard addition method and using certified reference materials. Zn was found in the highest amount and Tm in the least amount. Be, Co, Ag, Sb, La, and Ce exhibited statistically significant differences in the four age groups (20 to 41 years), whereas Ag and Ce showed a tendency to increase with age. Furthermore, our participants had notably higher As, Ni, and Co, levels and lower Zn levels compared to other populations. This is the first study that, in addition to analyzing essential and toxic trace elements, also provided an analysis of noble and rare earth elements in UCB plasma samples. Presented results can be used as a starting point or database for further studies, in terms of predicting the pregnancy outcome and postnatal development.
PB  - SpringerLink
T2  - Exposure and Health
T1  - Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood
VL  - 14
IS  - 3
SP  - 623
EP  - 633
DO  - 10.1007/s12403-021-00441-5
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Marija and Jagodić, Jovana and Miković, Željko and Jeremić, Ana and Perović, Milan and Manojlović, Dragan D.",
year = "2022",
abstract = "Due to the recognized harmful effects of some trace elements on fetal development after long-term exposures, it is first important to address their basic/physiological levels before monitoring toxicological effects and clinical outcomes on prenatal and postnatal health. This research aimed to define, for the first time, reference values for multiple (ultra) trace elements in umbilical cord blood (UCB) plasma samples of a notable number of healthy pregnant women (n = 125). All samples were collected during 2020–2021 and all participants were from Belgrade and two regions (Šumadija and Podunavlje). Following trace elements were enrolled in this study: essential (Mn, Co, Cu, Zn, Se, Mo), toxic (Be, Al, Ni, As, Rb, Sr, Ag, Cd, Sb, Ba, Tl, Pb, Th, U), rare earth (La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm), and noble metals (Ru, Rh, Pd, Re, Ir, Os, Pt). UCB plasma samples were diluted with suitable solvents and analyzed by inductively coupled plasma-mass spectrometry. The analytical technique used was validated by the standard addition method and using certified reference materials. Zn was found in the highest amount and Tm in the least amount. Be, Co, Ag, Sb, La, and Ce exhibited statistically significant differences in the four age groups (20 to 41 years), whereas Ag and Ce showed a tendency to increase with age. Furthermore, our participants had notably higher As, Ni, and Co, levels and lower Zn levels compared to other populations. This is the first study that, in addition to analyzing essential and toxic trace elements, also provided an analysis of noble and rare earth elements in UCB plasma samples. Presented results can be used as a starting point or database for further studies, in terms of predicting the pregnancy outcome and postnatal development.",
publisher = "SpringerLink",
journal = "Exposure and Health",
title = "Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood",
volume = "14",
number = "3",
pages = "623-633",
doi = "10.1007/s12403-021-00441-5"
}
Stojsavljević, A., Rovčanin, M., Jagodić, J., Miković, Ž., Jeremić, A., Perović, M.,& Manojlović, D. D.. (2022). Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood. in Exposure and Health
SpringerLink., 14(3), 623-633.
https://doi.org/10.1007/s12403-021-00441-5
Stojsavljević A, Rovčanin M, Jagodić J, Miković Ž, Jeremić A, Perović M, Manojlović DD. Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood. in Exposure and Health. 2022;14(3):623-633.
doi:10.1007/s12403-021-00441-5 .
Stojsavljević, Aleksandar, Rovčanin, Marija, Jagodić, Jovana, Miković, Željko, Jeremić, Ana, Perović, Milan, Manojlović, Dragan D., "Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood" in Exposure and Health, 14, no. 3 (2022):623-633,
https://doi.org/10.1007/s12403-021-00441-5 . .

Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy

Jagodić, Jovana; Pavlović, Slađan; Borković-Mitić, Slavica; Perović, Milan; Miković, Željko; Đurđić, Slađana Z.; Manojlović, Dragan D.; Stojsavljević, Aleksandar

(MDPI, 2022)

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Perović, Milan
AU  - Miković, Željko
AU  - Đurđić, Slađana Z.
AU  - Manojlović, Dragan D.
AU  - Stojsavljević, Aleksandar
PY  - 2022
UR  - https://www.mdpi.com/journal/ijms
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5522
AB  - With the ever-growing concern for human health and wellbeing, the prenatal period ofdevelopment requires special attention since fetuses can be exposed to various metals through themother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, andplacental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on thepotential transplacental transfer of these trace metals. Based on the obtained levels of investigatedelements in clinical samples, it was observed that all of the trace metals cross the placental barrierand reach the fetus. Furthermore, statistical analysis revealed significant differences in levels oftoxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinicalsamples. Correlation analysis highlighted As to be an element with levels that differed significantlybetween all tested samples. Principal component analysis (PCA) was used to enhance these findings.PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals indistinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible forthe clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera wasresponsible for the clustering of these samples. Overall, the findings of this study could contribute toa better understanding of transplacental transfer of these trace metals, and shed a light on overalllevels of metal exposure in the population of healthy pregnant women and their fetuses.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy
VL  - 23
IS  - 15
SP  - 8078
DO  - 10.3390/ijms23158078
ER  - 
@article{
author = "Jagodić, Jovana and Pavlović, Slađan and Borković-Mitić, Slavica and Perović, Milan and Miković, Željko and Đurđić, Slađana Z. and Manojlović, Dragan D. and Stojsavljević, Aleksandar",
year = "2022",
abstract = "With the ever-growing concern for human health and wellbeing, the prenatal period ofdevelopment requires special attention since fetuses can be exposed to various metals through themother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, andplacental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on thepotential transplacental transfer of these trace metals. Based on the obtained levels of investigatedelements in clinical samples, it was observed that all of the trace metals cross the placental barrierand reach the fetus. Furthermore, statistical analysis revealed significant differences in levels oftoxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinicalsamples. Correlation analysis highlighted As to be an element with levels that differed significantlybetween all tested samples. Principal component analysis (PCA) was used to enhance these findings.PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals indistinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible forthe clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera wasresponsible for the clustering of these samples. Overall, the findings of this study could contribute toa better understanding of transplacental transfer of these trace metals, and shed a light on overalllevels of metal exposure in the population of healthy pregnant women and their fetuses.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy",
volume = "23",
number = "15",
pages = "8078",
doi = "10.3390/ijms23158078"
}
Jagodić, J., Pavlović, S., Borković-Mitić, S., Perović, M., Miković, Ž., Đurđić, S. Z., Manojlović, D. D.,& Stojsavljević, A.. (2022). Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences
MDPI., 23(15), 8078.
https://doi.org/10.3390/ijms23158078
Jagodić J, Pavlović S, Borković-Mitić S, Perović M, Miković Ž, Đurđić SZ, Manojlović DD, Stojsavljević A. Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences. 2022;23(15):8078.
doi:10.3390/ijms23158078 .
Jagodić, Jovana, Pavlović, Slađan, Borković-Mitić, Slavica, Perović, Milan, Miković, Željko, Đurđić, Slađana Z., Manojlović, Dragan D., Stojsavljević, Aleksandar, "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy" in International Journal of Molecular Sciences, 23, no. 15 (2022):8078,
https://doi.org/10.3390/ijms23158078 . .
1

Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements

Stojsavljević, Aleksandar; Zečević, Nebojša; Mihailović, Mladen; Jagodić, Jovana; Đurđić, Slađana Z.; Perović, Milan; Manojlović, Dragan D.

(Elsevier, 2022)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Zečević, Nebojša
AU  - Mihailović, Mladen
AU  - Jagodić, Jovana
AU  - Đurđić, Slađana Z.
AU  - Perović, Milan
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5628
AB  - Background: As a consequence of the progressive decline in human semen quality in recent decades, modern epidemiological investigations have identified several trace elements that could be responsible for this phenomenon. However, their levels in semen have not been clearly elucidated, particularly for elements present in ultra-trace levels. Methods: We aimed to determine the levels of 39 (ultra)trace elements and 5 macroelements in human semen samples with confirmed normozoospermia using ICP-based techniques. The research was amplified by analyzing blood samples from the same participants. Results: Among the analyzed (ultra)trace elements in semen samples, Zn is the most and Tm is the least prominent. Zn levels in semen are so high that Zn should be considered as a macroelement in this matrix. The levels of Zn, Rh, Sm, Re, Ir, Tl, Na, and Ca were significantly higher in semen, while the levels of Cu, As, Rb, Gd, Sb, Tb, Tm, Lu, K, and Fe were significantly higher in blood. Correlation analysis of the levels of 44 individual elements in paired semen and blood samples revealed positive correlations between 43 of the elements, particularly for Tl and Pt. An exception was the negative correlation for Cu, which showed that its high level in semen is associated with a low level in blood and contrariwise. Conclusion: The reported data can be used as baseline levels/reference values for 44 elements in human semen. Furthermore, the findings of this study could be relevant for further consideration of male infertility.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements
VL  - 74
SP  - 127081
DO  - 10.1016/j.jtemb.2022.127081
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Zečević, Nebojša and Mihailović, Mladen and Jagodić, Jovana and Đurđić, Slađana Z. and Perović, Milan and Manojlović, Dragan D.",
year = "2022",
abstract = "Background: As a consequence of the progressive decline in human semen quality in recent decades, modern epidemiological investigations have identified several trace elements that could be responsible for this phenomenon. However, their levels in semen have not been clearly elucidated, particularly for elements present in ultra-trace levels. Methods: We aimed to determine the levels of 39 (ultra)trace elements and 5 macroelements in human semen samples with confirmed normozoospermia using ICP-based techniques. The research was amplified by analyzing blood samples from the same participants. Results: Among the analyzed (ultra)trace elements in semen samples, Zn is the most and Tm is the least prominent. Zn levels in semen are so high that Zn should be considered as a macroelement in this matrix. The levels of Zn, Rh, Sm, Re, Ir, Tl, Na, and Ca were significantly higher in semen, while the levels of Cu, As, Rb, Gd, Sb, Tb, Tm, Lu, K, and Fe were significantly higher in blood. Correlation analysis of the levels of 44 individual elements in paired semen and blood samples revealed positive correlations between 43 of the elements, particularly for Tl and Pt. An exception was the negative correlation for Cu, which showed that its high level in semen is associated with a low level in blood and contrariwise. Conclusion: The reported data can be used as baseline levels/reference values for 44 elements in human semen. Furthermore, the findings of this study could be relevant for further consideration of male infertility.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements",
volume = "74",
pages = "127081",
doi = "10.1016/j.jtemb.2022.127081"
}
Stojsavljević, A., Zečević, N., Mihailović, M., Jagodić, J., Đurđić, S. Z., Perović, M.,& Manojlović, D. D.. (2022). Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127081.
https://doi.org/10.1016/j.jtemb.2022.127081
Stojsavljević A, Zečević N, Mihailović M, Jagodić J, Đurđić SZ, Perović M, Manojlović DD. Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements. in Journal of Trace Elements in Medicine and Biology. 2022;74:127081.
doi:10.1016/j.jtemb.2022.127081 .
Stojsavljević, Aleksandar, Zečević, Nebojša, Mihailović, Mladen, Jagodić, Jovana, Đurđić, Slađana Z., Perović, Milan, Manojlović, Dragan D., "Elemental profiling of human semen with confirmed normozoospermia: Baseline levels for 44 elements" in Journal of Trace Elements in Medicine and Biology, 74 (2022):127081,
https://doi.org/10.1016/j.jtemb.2022.127081 . .

Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081.

Stojsavljević, Aleksandar; Zečević, Nebojša; Mihailović, Mladen; Jagodić, Jovana; Đurđić, Slađana Z.; Perović, Milan; Manojlović, Dragan D.

(Elsevier, 2022)

TY  - DATA
AU  - Stojsavljević, Aleksandar
AU  - Zečević, Nebojša
AU  - Mihailović, Mladen
AU  - Jagodić, Jovana
AU  - Đurđić, Slađana Z.
AU  - Perović, Milan
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5630
AB  - Background: As a consequence of the progressive decline in human semen quality in recent decades, modern epidemiological investigations have identified several trace elements that could be responsible for this phenomenon. However, their levels in semen have not been clearly elucidated, particularly for elements present in ultra-trace levels. Methods: We aimed to determine the levels of 39 (ultra)trace elements and 5 macroelements in human semen samples with confirmed normozoospermia using ICP-based techniques. The research was amplified by analyzing blood samples from the same participants. Results: Among the analyzed (ultra)trace elements in semen samples, Zn is the most and Tm is the least prominent. Zn levels in semen are so high that Zn should be considered as a macroelement in this matrix. The levels of Zn, Rh, Sm, Re, Ir, Tl, Na, and Ca were significantly higher in semen, while the levels of Cu, As, Rb, Gd, Sb, Tb, Tm, Lu, K, and Fe were significantly higher in blood. Correlation analysis of the levels of 44 individual elements in paired semen and blood samples revealed positive correlations between 43 of the elements, particularly for Tl and Pt. An exception was the negative correlation for Cu, which showed that its high level in semen is associated with a low level in blood and contrariwise. Conclusion: The reported data can be used as baseline levels/reference values for 44 elements in human semen. Furthermore, the findings of this study could be relevant for further consideration of male infertility.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081.
VL  - 74
SP  - 127081
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5630
ER  - 
@misc{
author = "Stojsavljević, Aleksandar and Zečević, Nebojša and Mihailović, Mladen and Jagodić, Jovana and Đurđić, Slađana Z. and Perović, Milan and Manojlović, Dragan D.",
year = "2022",
abstract = "Background: As a consequence of the progressive decline in human semen quality in recent decades, modern epidemiological investigations have identified several trace elements that could be responsible for this phenomenon. However, their levels in semen have not been clearly elucidated, particularly for elements present in ultra-trace levels. Methods: We aimed to determine the levels of 39 (ultra)trace elements and 5 macroelements in human semen samples with confirmed normozoospermia using ICP-based techniques. The research was amplified by analyzing blood samples from the same participants. Results: Among the analyzed (ultra)trace elements in semen samples, Zn is the most and Tm is the least prominent. Zn levels in semen are so high that Zn should be considered as a macroelement in this matrix. The levels of Zn, Rh, Sm, Re, Ir, Tl, Na, and Ca were significantly higher in semen, while the levels of Cu, As, Rb, Gd, Sb, Tb, Tm, Lu, K, and Fe were significantly higher in blood. Correlation analysis of the levels of 44 individual elements in paired semen and blood samples revealed positive correlations between 43 of the elements, particularly for Tl and Pt. An exception was the negative correlation for Cu, which showed that its high level in semen is associated with a low level in blood and contrariwise. Conclusion: The reported data can be used as baseline levels/reference values for 44 elements in human semen. Furthermore, the findings of this study could be relevant for further consideration of male infertility.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081.",
volume = "74",
pages = "127081",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5630"
}
Stojsavljević, A., Zečević, N., Mihailović, M., Jagodić, J., Đurđić, S. Z., Perović, M.,& Manojlović, D. D.. (2022). Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081.. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127081.
https://hdl.handle.net/21.15107/rcub_cherry_5630
Stojsavljević A, Zečević N, Mihailović M, Jagodić J, Đurđić SZ, Perović M, Manojlović DD. Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081.. in Journal of Trace Elements in Medicine and Biology. 2022;74:127081.
https://hdl.handle.net/21.15107/rcub_cherry_5630 .
Stojsavljević, Aleksandar, Zečević, Nebojša, Mihailović, Mladen, Jagodić, Jovana, Đurđić, Slađana Z., Perović, Milan, Manojlović, Dragan D., "Supplementary material for: Stojsavljević, A.; Zečević, N.; Mihailović, M.; Jagodić, J.; Đurđić, S.; Perović, M.; Manojlović, D. D. Elemental Profiling of Human Semen with Confirmed Normozoospermia: Baseline Levels for 44 Elements. Journal of Trace Elements in Medicine and Biology 2022, 74, 127081. https://doi.org/10.1016/j.jtemb.2022.127081." in Journal of Trace Elements in Medicine and Biology, 74 (2022):127081,
https://hdl.handle.net/21.15107/rcub_cherry_5630 .

A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels

Nešić, Andrijana; Stojsavljević, Aleksandar; Jagodić, Jovana; Čavić, Milena; Stefanović, Aleksandra; Manojlović, Dragan D.; Gavrović-Jankulović, Marija

(Elsevier, 2022)

TY  - JOUR
AU  - Nešić, Andrijana
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5636
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels
VL  - 74
SP  - 127079
DO  - 10.1016/j.jtemb.2022.127079
ER  - 
@article{
author = "Nešić, Andrijana and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels",
volume = "74",
pages = "127079",
doi = "10.1016/j.jtemb.2022.127079"
}
Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127079.
https://doi.org/10.1016/j.jtemb.2022.127079
Nešić A, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology. 2022;74:127079.
doi:10.1016/j.jtemb.2022.127079 .
Nešić, Andrijana, Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels" in Journal of Trace Elements in Medicine and Biology, 74 (2022):127079,
https://doi.org/10.1016/j.jtemb.2022.127079 . .

Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079

Nešić, Andrijana; Stojsavljević, Aleksandar; Jagodić, Jovana; Čavić, Milena; Stefanović, Aleksandra; Manojlović, Dragan D.; Gavrović-Jankulović, Marija

(Elsevier, 2022)

TY  - DATA
AU  - Nešić, Andrijana
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5658
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5658
ER  - 
@misc{
author = "Nešić, Andrijana and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5658"
}
Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_5658
Nešić A, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5658 .
Nešić, Andrijana, Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079" in Journal of Trace Elements in Medicine and Biology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5658 .

Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides

Dinić, Ivana; Vuković, Marina; Rabanal, Maria Eugenia; Mančić, Lidija

(Belgrade : Materials Research Society of Serbia, 2022)

TY  - CONF
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Rabanal, Maria Eugenia
AU  - Mančić, Lidija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5743
AB  - Over the last few years there has been a great interest in developing the efficient methodologies for the preparation of rare earth doped up-converting nanoparticles (UCNPs), which exhibit emission in visible part of spectra when excited by the near infrared (NIR) radiation. Due to this unique optical property, as well as the high chemical stability they could be used either as security or fluorescent biological labels. Depending on the host crystal structure and choice of the dopant, these could exhibit different luminescence response. In this work we represent the effect which hydro/solvo thermal treatment and simple co-precipitation have on structural, morphological and optical properties of NaYF4:Yb,Er UCNPs. We showed that pure cubic phase crystallizes in monodisperse spherical nanoparticles obtained through hydro/solvothermal synthesis, while co-precipitation and annealing at 500°C for 5 hours leads to the obtaining of agglomerated microparticles with hexagonal phase. Due to the difference in size and morphology of the samples, the different up-conversion mechanisms are established upon the excitation by the infrared light (λ = 978 nm), intensifying green emission (2H11/2,4S3/2 →4I15/2 ) in sample obtained through hydro/solvo thermal synthesis.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Program and The Book of abstracts / Twenty-third Annual Conference YUCOMAT 2022 & Twelfth World Round Table Conference on Sintering XII WRTCS 2022, Herceg Novi, Montenegro, August 29 - September 2, 2022
T1  - Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides
SP  - 126
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_dais_13595
ER  - 
@conference{
author = "Dinić, Ivana and Vuković, Marina and Rabanal, Maria Eugenia and Mančić, Lidija",
year = "2022",
abstract = "Over the last few years there has been a great interest in developing the efficient methodologies for the preparation of rare earth doped up-converting nanoparticles (UCNPs), which exhibit emission in visible part of spectra when excited by the near infrared (NIR) radiation. Due to this unique optical property, as well as the high chemical stability they could be used either as security or fluorescent biological labels. Depending on the host crystal structure and choice of the dopant, these could exhibit different luminescence response. In this work we represent the effect which hydro/solvo thermal treatment and simple co-precipitation have on structural, morphological and optical properties of NaYF4:Yb,Er UCNPs. We showed that pure cubic phase crystallizes in monodisperse spherical nanoparticles obtained through hydro/solvothermal synthesis, while co-precipitation and annealing at 500°C for 5 hours leads to the obtaining of agglomerated microparticles with hexagonal phase. Due to the difference in size and morphology of the samples, the different up-conversion mechanisms are established upon the excitation by the infrared light (λ = 978 nm), intensifying green emission (2H11/2,4S3/2 →4I15/2 ) in sample obtained through hydro/solvo thermal synthesis.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Program and The Book of abstracts / Twenty-third Annual Conference YUCOMAT 2022 & Twelfth World Round Table Conference on Sintering XII WRTCS 2022, Herceg Novi, Montenegro, August 29 - September 2, 2022",
title = "Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_dais_13595"
}
Dinić, I., Vuković, M., Rabanal, M. E.,& Mančić, L.. (2022). Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides. in Program and The Book of abstracts / Twenty-third Annual Conference YUCOMAT 2022 & Twelfth World Round Table Conference on Sintering XII WRTCS 2022, Herceg Novi, Montenegro, August 29 - September 2, 2022
Belgrade : Materials Research Society of Serbia., 126-126.
https://hdl.handle.net/21.15107/rcub_dais_13595
Dinić I, Vuković M, Rabanal ME, Mančić L. Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides. in Program and The Book of abstracts / Twenty-third Annual Conference YUCOMAT 2022 & Twelfth World Round Table Conference on Sintering XII WRTCS 2022, Herceg Novi, Montenegro, August 29 - September 2, 2022. 2022;:126-126.
https://hdl.handle.net/21.15107/rcub_dais_13595 .
Dinić, Ivana, Vuković, Marina, Rabanal, Maria Eugenia, Mančić, Lidija, "Influence of different synthesis methods on morphological and optical properties of the rare earth doped fluorides" in Program and The Book of abstracts / Twenty-third Annual Conference YUCOMAT 2022 & Twelfth World Round Table Conference on Sintering XII WRTCS 2022, Herceg Novi, Montenegro, August 29 - September 2, 2022 (2022):126-126,
https://hdl.handle.net/21.15107/rcub_dais_13595 .

Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?

FIlipović, Lidija; Kojadinović, Milica I.; Popović, Milica M.

(Elsevier, 2022)

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4989
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 
@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}
FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057
FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .
FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?

FIlipović, Lidija; Kojadinović, Milica I.; Popović, Milica M.

(Elsevier, 2022)

TY  - JOUR
AU  - FIlipović, Lidija
AU  - Kojadinović, Milica I.
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4997
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?
VL  - 68
SP  - 103057
DO  - 10.1016/j.jddst.2021.103057
ER  - 
@article{
author = "FIlipović, Lidija and Kojadinović, Milica I. and Popović, Milica M.",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?",
volume = "68",
pages = "103057",
doi = "10.1016/j.jddst.2021.103057"
}
FIlipović, L., Kojadinović, M. I.,& Popović, M. M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057
FIlipović L, Kojadinović MI, Popović MM. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .
FIlipović, Lidija, Kojadinović, Milica I., Popović, Milica M., "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand  and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer

Filipović, Lidija; Spasojević, Milica; Prodanović, Radivoje; Korać, Aleksandra; Matijaševic, Suzana; Brajušković, Goran; de Marco, Ario; Popović, Milica M.

(Elsevier, 2022)

TY  - JOUR
AU  - Filipović, Lidija
AU  - Spasojević, Milica
AU  - Prodanović, Radivoje
AU  - Korać, Aleksandra
AU  - Matijaševic, Suzana
AU  - Brajušković, Goran
AU  - de Marco, Ario
AU  - Popović, Milica M.
PY  - 2022
UR  - https://pubmed.ncbi.nlm.nih.gov/35301156/
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5152
AB  - Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.
PB  - Elsevier
T2  - New Biotechnology
T1  - Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer
VL  - 69
SP  - 36
EP  - 48
DO  - 10.1016/j.nbt.2022.03.001
ER  - 
@article{
author = "Filipović, Lidija and Spasojević, Milica and Prodanović, Radivoje and Korać, Aleksandra and Matijaševic, Suzana and Brajušković, Goran and de Marco, Ario and Popović, Milica M.",
year = "2022",
abstract = "Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer",
volume = "69",
pages = "36-48",
doi = "10.1016/j.nbt.2022.03.001"
}
Filipović, L., Spasojević, M., Prodanović, R., Korać, A., Matijaševic, S., Brajušković, G., de Marco, A.,& Popović, M. M.. (2022). Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer. in New Biotechnology
Elsevier., 69, 36-48.
https://doi.org/10.1016/j.nbt.2022.03.001
Filipović L, Spasojević M, Prodanović R, Korać A, Matijaševic S, Brajušković G, de Marco A, Popović MM. Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer. in New Biotechnology. 2022;69:36-48.
doi:10.1016/j.nbt.2022.03.001 .
Filipović, Lidija, Spasojević, Milica, Prodanović, Radivoje, Korać, Aleksandra, Matijaševic, Suzana, Brajušković, Goran, de Marco, Ario, Popović, Milica M., "Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer" in New Biotechnology, 69 (2022):36-48,
https://doi.org/10.1016/j.nbt.2022.03.001 . .
3
4

Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.

Filipović, Lidija; Spasojević, Milica; Prodanović, Radivoje; Korać, Aleksandra; Matijaševic, Suzana; Brajušković, Goran; de Marco, Ario; Popović, Milica M.

(Elsevier, 2022)

TY  - DATA
AU  - Filipović, Lidija
AU  - Spasojević, Milica
AU  - Prodanović, Radivoje
AU  - Korać, Aleksandra
AU  - Matijaševic, Suzana
AU  - Brajušković, Goran
AU  - de Marco, Ario
AU  - Popović, Milica M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5152
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5153
AB  - Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.
PB  - Elsevier
T2  - New Biotechnology
T1  - Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5153
ER  - 
@misc{
author = "Filipović, Lidija and Spasojević, Milica and Prodanović, Radivoje and Korać, Aleksandra and Matijaševic, Suzana and Brajušković, Goran and de Marco, Ario and Popović, Milica M.",
year = "2022",
abstract = "Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purification alternative to obtain homogeneous EV samples. An easy-to-operate chromatography system was set-up for the purification of intact EVs based on a single domain (VHH) antibodies-copolymer matrix suitable for biological samples as different as conditioned cell culture medium and human plasma. Methacrylate-based copolymer is a porous solid support, the chemical versatility of which enables its efficient functionalization with VHHs. The combined analyses of morphological features and biomarker (CD9, CD63 and CD81) presence indicated that the recovered EVs were exosomes. The lipoprotein markers APO-A1 and APO-B were both negative in tested samples. This is the first report demonstrating the successful application of spherical porous methacrylate-based copolymer coupled with VHHs for the exosome isolation from biological fluids. This inexpensive immunoaffinity method has the potential to be applied for the isolation of EVs belonging to different morphological and physiological classes.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5153"
}
Filipović, L., Spasojević, M., Prodanović, R., Korać, A., Matijaševic, S., Brajušković, G., de Marco, A.,& Popović, M. M.. (2022). Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.. in New Biotechnology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_5153
Filipović L, Spasojević M, Prodanović R, Korać A, Matijaševic S, Brajušković G, de Marco A, Popović MM. Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001.. in New Biotechnology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5153 .
Filipović, Lidija, Spasojević, Milica, Prodanović, Radivoje, Korać, Aleksandra, Matijaševic, Suzana, Brajušković, Goran, de Marco, Ario, Popović, Milica M., "Supplementary information for the article: Filipović, L.; Spasojević, M.; Prodanović, R.; Korać, A.; Matijaševic, S.; Brajušković, G.; de Marco, A.; Popović, M. Affinity-Based Isolation of Extracellular Vesicles by Means of Single-Domain Antibodies Bound to Macroporous Methacrylate-Based Copolymer. New Biotechnology 2022, 69, 36–48. https://doi.org/10.1016/j.nbt.2022.03.001." in New Biotechnology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5153 .

The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure

Mijin, Nemanja D.; Milošević, Jelica; Filipović, Nenad R.; Mitić, Dragana; Anđelković, Katarina; Polović, Natalija Đ.; Todorović, Tamara R.

(Serbian Chemical Society, 2022)

TY  - JOUR
AU  - Mijin, Nemanja D.
AU  - Milošević, Jelica
AU  - Filipović, Nenad R.
AU  - Mitić, Dragana
AU  - Anđelković, Katarina
AU  - Polović, Natalija Đ.
AU  - Todorović, Tamara R.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5686
AB  - Previously, the cytotoxic actions of five Pd(II) complexes with bidentate N-heteroaromatic chelators (complexes 1–5) on a palette of several cancer
cell lines were investigated. However, the results of the cytotoxic activity did
not correlate with the hydrophobic character of the complexes. To gain further
insight into the structure–activity relationship, essential for the design of novel
potential drugs, other factors, such as non-specific interactions with cellular
proteins, have to be taken into account. To explore the potential non-specific
influence of the complexes on protein structures, ovalbumin (OVA) was
chosen as a model system to mimic cellular non-specific crowding environments with high protein concentrations. A Fourier-transform infrared spectroscopy study implied that the binding of 3 and 4 led to only moderate alternations in the secondary structures of the protein, without the possibility to penetrate into hydrophobic core of the protein and disruption of protein native fold.
Contrary, the effect of complex 5 on OVA secondary structures was concentration-dependent. While the lower concentration of complex 5 had no effect
on OVA structure, a doubled concentration of complex 5 led to complete disruption of the content native-like secondary structures. The concentration-dependent effect of complex 5 on the changes in secondary structures and considerable increase in the exposure of OVA hydrophobic surfaces to water may
be related to a potential crosslinking that leads to OVA aggregation.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure
VL  - 87
IS  - 10
SP  - 1143
SP  - 1156
DO  - 10.2298/JSC220518050M
ER  - 
@article{
author = "Mijin, Nemanja D. and Milošević, Jelica and Filipović, Nenad R. and Mitić, Dragana and Anđelković, Katarina and Polović, Natalija Đ. and Todorović, Tamara R.",
year = "2022",
abstract = "Previously, the cytotoxic actions of five Pd(II) complexes with bidentate N-heteroaromatic chelators (complexes 1–5) on a palette of several cancer
cell lines were investigated. However, the results of the cytotoxic activity did
not correlate with the hydrophobic character of the complexes. To gain further
insight into the structure–activity relationship, essential for the design of novel
potential drugs, other factors, such as non-specific interactions with cellular
proteins, have to be taken into account. To explore the potential non-specific
influence of the complexes on protein structures, ovalbumin (OVA) was
chosen as a model system to mimic cellular non-specific crowding environments with high protein concentrations. A Fourier-transform infrared spectroscopy study implied that the binding of 3 and 4 led to only moderate alternations in the secondary structures of the protein, without the possibility to penetrate into hydrophobic core of the protein and disruption of protein native fold.
Contrary, the effect of complex 5 on OVA secondary structures was concentration-dependent. While the lower concentration of complex 5 had no effect
on OVA structure, a doubled concentration of complex 5 led to complete disruption of the content native-like secondary structures. The concentration-dependent effect of complex 5 on the changes in secondary structures and considerable increase in the exposure of OVA hydrophobic surfaces to water may
be related to a potential crosslinking that leads to OVA aggregation.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure",
volume = "87",
number = "10",
pages = "1143-1156",
doi = "10.2298/JSC220518050M"
}
Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ.,& Todorović, T. R.. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(10), 1143.
https://doi.org/10.2298/JSC220518050M
Mijin ND, Milošević J, Filipović NR, Mitić D, Anđelković K, Polović NĐ, Todorović TR. The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. in Journal of the Serbian Chemical Society. 2022;87(10):1143.
doi:10.2298/JSC220518050M .
Mijin, Nemanja D., Milošević, Jelica, Filipović, Nenad R., Mitić, Dragana, Anđelković, Katarina, Polović, Natalija Đ., Todorović, Tamara R., "The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure" in Journal of the Serbian Chemical Society, 87, no. 10 (2022):1143,
https://doi.org/10.2298/JSC220518050M . .

Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes

Kokić, Branislav; Selaković, Života; Nikolić, Andrea M.; Andrijević, Ana; Anđelković, Boban D.; Ajdačić, Vladimir; Opsenica, Igor

(Wiley, 2022)

TY  - JOUR
AU  - Kokić, Branislav
AU  - Selaković, Života
AU  - Nikolić, Andrea M.
AU  - Andrijević, Ana
AU  - Anđelković, Boban D.
AU  - Ajdačić, Vladimir
AU  - Opsenica, Igor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5680
AB  - Herein, we report a low-valent cobalt-catalyzed deprotection of allyloxyarenes. The method displays a broad substrate scope and good functional group tolerance, granting its utilization on more complex substrates, including O-allylated derivatives of natural products and drugs. Based on comprehensive experiments, a plausible mechanistic pathway for the low-valent cobalt-catalyzed O-deallylation of allyloxyarenes is proposed.
PB  - Wiley
T2  - European Journal of Organic Chemistry
T1  - Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes
VL  - 43
SP  - e202201112
DO  - 10.1002/ejoc.202201112
ER  - 
@article{
author = "Kokić, Branislav and Selaković, Života and Nikolić, Andrea M. and Andrijević, Ana and Anđelković, Boban D. and Ajdačić, Vladimir and Opsenica, Igor",
year = "2022",
abstract = "Herein, we report a low-valent cobalt-catalyzed deprotection of allyloxyarenes. The method displays a broad substrate scope and good functional group tolerance, granting its utilization on more complex substrates, including O-allylated derivatives of natural products and drugs. Based on comprehensive experiments, a plausible mechanistic pathway for the low-valent cobalt-catalyzed O-deallylation of allyloxyarenes is proposed.",
publisher = "Wiley",
journal = "European Journal of Organic Chemistry",
title = "Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes",
volume = "43",
pages = "e202201112",
doi = "10.1002/ejoc.202201112"
}
Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V.,& Opsenica, I.. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. in European Journal of Organic Chemistry
Wiley., 43, e202201112.
https://doi.org/10.1002/ejoc.202201112
Kokić B, Selaković Ž, Nikolić AM, Andrijević A, Anđelković BD, Ajdačić V, Opsenica I. Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. in European Journal of Organic Chemistry. 2022;43:e202201112.
doi:10.1002/ejoc.202201112 .
Kokić, Branislav, Selaković, Života, Nikolić, Andrea M., Andrijević, Ana, Anđelković, Boban D., Ajdačić, Vladimir, Opsenica, Igor, "Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes" in European Journal of Organic Chemistry, 43 (2022):e202201112,
https://doi.org/10.1002/ejoc.202201112 . .

Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112

Kokić, Branislav; Selaković, Života; Nikolić, Andrea M.; Andrijević, Ana; Anđelković, Boban D.; Ajdačić, Vladimir; Opsenica, Igor

(Wiley, 2022)

TY  - DATA
AU  - Kokić, Branislav
AU  - Selaković, Života
AU  - Nikolić, Andrea M.
AU  - Andrijević, Ana
AU  - Anđelković, Boban D.
AU  - Ajdačić, Vladimir
AU  - Opsenica, Igor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5706
AB  - Herein, we report a low-valent cobalt-catalyzed deprotection of allyloxyarenes. The method displays a broad substrate scope and good functional group tolerance, granting its utilization on more complex substrates, including O-allylated derivatives of natural products and drugs. Based on comprehensive experiments, a plausible mechanistic pathway for the low-valent cobalt-catalyzed O-deallylation of allyloxyarenes is proposed.
PB  - Wiley
T2  - European Journal of Organic Chemistry
T1  - Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112
VL  - 43
SP  - e202201112
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5706
ER  - 
@misc{
author = "Kokić, Branislav and Selaković, Života and Nikolić, Andrea M. and Andrijević, Ana and Anđelković, Boban D. and Ajdačić, Vladimir and Opsenica, Igor",
year = "2022",
abstract = "Herein, we report a low-valent cobalt-catalyzed deprotection of allyloxyarenes. The method displays a broad substrate scope and good functional group tolerance, granting its utilization on more complex substrates, including O-allylated derivatives of natural products and drugs. Based on comprehensive experiments, a plausible mechanistic pathway for the low-valent cobalt-catalyzed O-deallylation of allyloxyarenes is proposed.",
publisher = "Wiley",
journal = "European Journal of Organic Chemistry",
title = "Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112",
volume = "43",
pages = "e202201112",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5706"
}
Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V.,& Opsenica, I.. (2022). Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112. in European Journal of Organic Chemistry
Wiley., 43, e202201112.
https://hdl.handle.net/21.15107/rcub_cherry_5706
Kokić B, Selaković Ž, Nikolić AM, Andrijević A, Anđelković BD, Ajdačić V, Opsenica I. Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112. in European Journal of Organic Chemistry. 2022;43:e202201112.
https://hdl.handle.net/21.15107/rcub_cherry_5706 .
Kokić, Branislav, Selaković, Života, Nikolić, Andrea M., Andrijević, Ana, Anđelković, Boban D., Ajdačić, Vladimir, Opsenica, Igor, "Supplementary material for: Kokić, B., Selaković, Ž., Nikolić, A. M., Andrijević, A., Anđelković, B. D., Ajdačić, V., & Opsenica, I. (2022). Low-Valent Cobalt-Catalyzed Deprotection of Allyloxyarenes. European Journal of Organic Chemistry, 43, e202201112. https://doi.org/10.1002/ejoc.202201112" in European Journal of Organic Chemistry, 43 (2022):e202201112,
https://hdl.handle.net/21.15107/rcub_cherry_5706 .

Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068

Manojlović-Stojanoski, Milica; Borković-Mitić, Slavica; Nestorović, Nataša; Ristić, Nataša; Trifunović, Svetlana; Stevanović, Magdalena; Filipović, Nenad; Stojsavljević, Aleksandar; Pavlović, Slađan

(MDPI, 2022)

TY  - DATA
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5705
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068
VL  - 23
IS  - 21
SP  - 13068
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5705
ER  - 
@misc{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068",
volume = "23",
number = "21",
pages = "13068",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5705"
}
Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068. in International Journal of Molecular Sciences
MDPI., 23(21), 13068.
https://hdl.handle.net/21.15107/rcub_cherry_5705
Manojlović-Stojanoski M, Borković-Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068. in International Journal of Molecular Sciences. 2022;23(21):13068.
https://hdl.handle.net/21.15107/rcub_cherry_5705 .
Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "Supplementary material for: Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A., & Pavlović, S. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. International Journal of Molecular Sciences, 23(21), 13068. https://doi.org/10.3390/ijms232113068" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://hdl.handle.net/21.15107/rcub_cherry_5705 .

Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M

Mijin, Nemanja D.; Milošević, Jelica; Filipović, Nenad R.; Mitić, Dragana; Anđelković, Katarina; Polović, Natalija Đ.; Todorović, Tamara R.

(Serbian Chemical Society, 2022)

TY  - DATA
AU  - Mijin, Nemanja D.
AU  - Milošević, Jelica
AU  - Filipović, Nenad R.
AU  - Mitić, Dragana
AU  - Anđelković, Katarina
AU  - Polović, Natalija Đ.
AU  - Todorović, Tamara R.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5707
AB  - Previously, the cytotoxic actions of five Pd(II) complexes with bidentate N-heteroaromatic chelators (complexes 1–5) on a palette of several cancercell lines were investigated. However, the results of the cytotoxic activity didnot correlate with the hydrophobic character of the complexes. To gain furtherinsight into the structure–activity relationship, essential for the design of novelpotential drugs, other factors, such as non-specific interactions with cellularproteins, have to be taken into account. To explore the potential non-specificinfluence of the complexes on protein structures, ovalbumin (OVA) waschosen as a model system to mimic cellular non-specific crowding environments with high protein concentrations. A Fourier-transform infrared spectroscopy study implied that the binding of 3 and 4 led to only moderate alternations in the secondary structures of the protein, without the possibility to penetrate into hydrophobic core of the protein and disruption of protein native fold.Contrary, the effect of complex 5 on OVA secondary structures was concentration-dependent. While the lower concentration of complex 5 had no effecton OVA structure, a doubled concentration of complex 5 led to complete disruption of the content native-like secondary structures. The concentration-dependent effect of complex 5 on the changes in secondary structures and considerable increase in the exposure of OVA hydrophobic surfaces to water maybe related to a potential crosslinking that leads to OVA aggregation.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M
VL  - 87
IS  - 10
SP  - 1143
SP  - 1156
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5707
ER  - 
@misc{
author = "Mijin, Nemanja D. and Milošević, Jelica and Filipović, Nenad R. and Mitić, Dragana and Anđelković, Katarina and Polović, Natalija Đ. and Todorović, Tamara R.",
year = "2022",
abstract = "Previously, the cytotoxic actions of five Pd(II) complexes with bidentate N-heteroaromatic chelators (complexes 1–5) on a palette of several cancercell lines were investigated. However, the results of the cytotoxic activity didnot correlate with the hydrophobic character of the complexes. To gain furtherinsight into the structure–activity relationship, essential for the design of novelpotential drugs, other factors, such as non-specific interactions with cellularproteins, have to be taken into account. To explore the potential non-specificinfluence of the complexes on protein structures, ovalbumin (OVA) waschosen as a model system to mimic cellular non-specific crowding environments with high protein concentrations. A Fourier-transform infrared spectroscopy study implied that the binding of 3 and 4 led to only moderate alternations in the secondary structures of the protein, without the possibility to penetrate into hydrophobic core of the protein and disruption of protein native fold.Contrary, the effect of complex 5 on OVA secondary structures was concentration-dependent. While the lower concentration of complex 5 had no effecton OVA structure, a doubled concentration of complex 5 led to complete disruption of the content native-like secondary structures. The concentration-dependent effect of complex 5 on the changes in secondary structures and considerable increase in the exposure of OVA hydrophobic surfaces to water maybe related to a potential crosslinking that leads to OVA aggregation.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M",
volume = "87",
number = "10",
pages = "1143-1156",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5707"
}
Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ.,& Todorović, T. R.. (2022). Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 87(10), 1143.
https://hdl.handle.net/21.15107/rcub_cherry_5707
Mijin ND, Milošević J, Filipović NR, Mitić D, Anđelković K, Polović NĐ, Todorović TR. Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M. in Journal of the Serbian Chemical Society. 2022;87(10):1143.
https://hdl.handle.net/21.15107/rcub_cherry_5707 .
Mijin, Nemanja D., Milošević, Jelica, Filipović, Nenad R., Mitić, Dragana, Anđelković, Katarina, Polović, Natalija Đ., Todorović, Tamara R., "Supplementary material for: Mijin, N. D., Milošević, J., Filipović, N. R., Mitić, D., Anđelković, K., Polović, N. Đ., & Todorović, T. R. (2022). The effect of non-specific binding of Pd(II) complexes with N-heteroaromatic hydrazone ligands on the protein structure. Journal of the Serbian Chemical Society, 87(10), 1143. https://doi.org/10.2298/JSC220518050M" in Journal of the Serbian Chemical Society, 87, no. 10 (2022):1143,
https://hdl.handle.net/21.15107/rcub_cherry_5707 .

Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity

Nikolić, Vesna; Đokić, Jovana; Kamberović, Željko; Marinković, Aleksandar; Jevtić, Sanja O.; Anđić, Zoran

(2022)

TY  - JOUR
AU  - Nikolić, Vesna
AU  - Đokić, Jovana
AU  - Kamberović, Željko
AU  - Marinković, Aleksandar
AU  - Jevtić, Sanja O.
AU  - Anđić, Zoran
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5514
AB  - The aim of this study was to investigate a possibility of synthesis of porous ceramics with controlled open porosity, which could be used as sorbents and catalyst supports. Two organic additives were used to obtain open porosity: polystyrene beads and cellulose fibers, which are mixed with kaolin clay powder and the appropriate water content. Samples were sintered at 1050 oC for 1 h. Characterization of the obtained products included X-ray powder diffraction analysis (XRPD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and elemental CHNS analysis. In addition, porosity was examined by quantification of visual information. The specific surface areas were determined by the Brunauer–Emmett–Teller (BET) method. Also, density and compressive strength of the obtained samples were assessed. It was determined that by sintering, the organic component completely leaves the system. For samples prepared with polystyrene beads and with cellulose fibers, satisfactory mechanical properties were obtained: compressive strengths were 1.42 and 1.56 MPa, respectively. It was noted that significantly higher open porosity was obtained by using polystyrene beads as a sacrificial template (porosity of ̴56 %) instead of cellulose fibers (porosity of ̴6 %).
T2  - Hemijska Industrija
T1  - Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity
VL  - 76
IS  - 2
SP  - 87
EP  - 95
DO  - 10.2298/HEMIND210809005N
ER  - 
@article{
author = "Nikolić, Vesna and Đokić, Jovana and Kamberović, Željko and Marinković, Aleksandar and Jevtić, Sanja O. and Anđić, Zoran",
year = "2022",
abstract = "The aim of this study was to investigate a possibility of synthesis of porous ceramics with controlled open porosity, which could be used as sorbents and catalyst supports. Two organic additives were used to obtain open porosity: polystyrene beads and cellulose fibers, which are mixed with kaolin clay powder and the appropriate water content. Samples were sintered at 1050 oC for 1 h. Characterization of the obtained products included X-ray powder diffraction analysis (XRPD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and elemental CHNS analysis. In addition, porosity was examined by quantification of visual information. The specific surface areas were determined by the Brunauer–Emmett–Teller (BET) method. Also, density and compressive strength of the obtained samples were assessed. It was determined that by sintering, the organic component completely leaves the system. For samples prepared with polystyrene beads and with cellulose fibers, satisfactory mechanical properties were obtained: compressive strengths were 1.42 and 1.56 MPa, respectively. It was noted that significantly higher open porosity was obtained by using polystyrene beads as a sacrificial template (porosity of ̴56 %) instead of cellulose fibers (porosity of ̴6 %).",
journal = "Hemijska Industrija",
title = "Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity",
volume = "76",
number = "2",
pages = "87-95",
doi = "10.2298/HEMIND210809005N"
}
Nikolić, V., Đokić, J., Kamberović, Ž., Marinković, A., Jevtić, S. O.,& Anđić, Z.. (2022). Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity. in Hemijska Industrija, 76(2), 87-95.
https://doi.org/10.2298/HEMIND210809005N
Nikolić V, Đokić J, Kamberović Ž, Marinković A, Jevtić SO, Anđić Z. Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity. in Hemijska Industrija. 2022;76(2):87-95.
doi:10.2298/HEMIND210809005N .
Nikolić, Vesna, Đokić, Jovana, Kamberović, Željko, Marinković, Aleksandar, Jevtić, Sanja O., Anđić, Zoran, "Investigating possibilities for synthesis of novel sorbents and catalyst carriers based on ceramics with controlled open porosity" in Hemijska Industrija, 76, no. 2 (2022):87-95,
https://doi.org/10.2298/HEMIND210809005N . .

Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651

Horvacki, Nikola; Andrić, Filip; Gašić, Uroš M.; Đurović, Dejan; Tešić, Živoslav Lj.; Fotirić-Akšić, Milica M.; Milojković-Opsenica, Dušanka

(MDPI, 2022)

TY  - DATA
AU  - Horvacki, Nikola
AU  - Andrić, Filip
AU  - Gašić, Uroš M.
AU  - Đurović, Dejan
AU  - Tešić, Živoslav Lj.
AU  - Fotirić-Akšić, Milica M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5701
AB  - Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.
PB  - MDPI
T2  - Molecules
T1  - Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651
VL  - 27
IS  - 21
SP  - 7651
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5701
ER  - 
@misc{
author = "Horvacki, Nikola and Andrić, Filip and Gašić, Uroš M. and Đurović, Dejan and Tešić, Živoslav Lj. and Fotirić-Akšić, Milica M. and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Domesticated international (standard) apple cultivars, together with resistant apple cultivars are the core of the Serbian apple production. Furthermore, autochthonous cultivars are characterized by a good adaptability to the local environmental conditions and represent a valuable source of genetic variability, as well as an important source of the gene pool for further breeding programs. Additionally, they show a higher phenolic content and a stronger antioxidant activity, in comparison to commercial cultivars. Therefore, they are more likely to be used as a functional food. The subjects of this study were seventeen samples of fruits and leaves from autochthonous apple cultivars, five international standard cultivars, and six resistant apple cultivars. The phenolic profile was determined using ultra-high performance liquid chromatography (UHPLC), coupled with a diode array detector and a TSQ Quantum Access Max triple-quadrupole mass spectrometer. A total of twenty compounds were quantified in the samples. Most of the analyzed phenolics were detected in higher amounts in the peel, compared to the mesocarp. The results of the multivariate analysis of variance (MANOVA) indicate that 5-O-caffeoylquinic acid is present in the highest amount in the mesocarp, while in the peel and leaves, quercetin-glycosides were detected in the highest amount. According to the MANOVA: phloretin, phlorizin, 5-O-caffeoylquinic acid, kaempferol, and p-coumaric acid are present in significantly higher levels in the autochthonous cultivars, compared to the standard and resistant ones (in both fruits and leaves). Therefore, these compounds can be used as chemical tracers of the apple varietal origin.",
publisher = "MDPI",
journal = "Molecules",
title = "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651",
volume = "27",
number = "21",
pages = "7651",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5701"
}
Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž. Lj., Fotirić-Akšić, M. M.,& Milojković-Opsenica, D.. (2022). Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules
MDPI., 27(21), 7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701
Horvacki N, Andrić F, Gašić UM, Đurović D, Tešić ŽL, Fotirić-Akšić MM, Milojković-Opsenica D. Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651. in Molecules. 2022;27(21):7651.
https://hdl.handle.net/21.15107/rcub_cherry_5701 .
Horvacki, Nikola, Andrić, Filip, Gašić, Uroš M., Đurović, Dejan, Tešić, Živoslav Lj., Fotirić-Akšić, Milica M., Milojković-Opsenica, Dušanka, "Supplementary material for the article: Horvacki, N., Andrić, F., Gašić, U. M., Đurović, D., Tešić, Ž., Fotirić-Akšić, M. M., & Milojković-Opsenica, D. (2022). Phenolic Compounds as Phytochemical Tracers of Varietal Origin of Some Autochthonous Apple Cultivars Grown in Serbia. Molecules, 27(21), 7651. https://doi.org/10.3390/molecules27217651" in Molecules, 27, no. 21 (2022):7651,
https://hdl.handle.net/21.15107/rcub_cherry_5701 .
1

Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products

Selaković, Života; Nikolić, Andrea; Ajdačić, Vladimir; Opsenica, Igor

(Wiley, 2022)

TY  - JOUR
AU  - Selaković, Života
AU  - Nikolić, Andrea
AU  - Ajdačić, Vladimir
AU  - Opsenica, Igor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4871
AB  - Decarbonylation is an invaluable reaction, utilized by nature and chemists alike. In different life forms, such as prokaryotes, plants and animals, this transformation is catalyzed by aldehyde decarbonylases. In the laboratory, the transition metal-catalyzed (TMC) decarbonylation, which was first achieved in 1959, is by and large the dominant way for conducting this reaction. The carbon-carbon bond cleavage is most often made possible by RhCl(PPh3)3 i. e. Wilkinson's catalyst, but other metals are also used, both in academia and in industry. In this review, we chose to present the applications of TMC decarbonylation in the synthesis of natural products and their derivatives. More than 30 examples are showcased and categorized into three categories based on the essence of the role of the aldehyde group in the synthesis. A short outlook is given in the end, listing the different advantages and disadvantages of Wilkinson's catalyst, as well as offering a brief prospect for the future.
PB  - Wiley
T2  - European Journal of Organic Chemistry
T1  - Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products
VL  - 2022
IS  - 1
SP  - e202101265
DO  - 10.1002/ejoc.202101265
ER  - 
@article{
author = "Selaković, Života and Nikolić, Andrea and Ajdačić, Vladimir and Opsenica, Igor",
year = "2022",
abstract = "Decarbonylation is an invaluable reaction, utilized by nature and chemists alike. In different life forms, such as prokaryotes, plants and animals, this transformation is catalyzed by aldehyde decarbonylases. In the laboratory, the transition metal-catalyzed (TMC) decarbonylation, which was first achieved in 1959, is by and large the dominant way for conducting this reaction. The carbon-carbon bond cleavage is most often made possible by RhCl(PPh3)3 i. e. Wilkinson's catalyst, but other metals are also used, both in academia and in industry. In this review, we chose to present the applications of TMC decarbonylation in the synthesis of natural products and their derivatives. More than 30 examples are showcased and categorized into three categories based on the essence of the role of the aldehyde group in the synthesis. A short outlook is given in the end, listing the different advantages and disadvantages of Wilkinson's catalyst, as well as offering a brief prospect for the future.",
publisher = "Wiley",
journal = "European Journal of Organic Chemistry",
title = "Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products",
volume = "2022",
number = "1",
pages = "e202101265",
doi = "10.1002/ejoc.202101265"
}
Selaković, Ž., Nikolić, A., Ajdačić, V.,& Opsenica, I.. (2022). Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products. in European Journal of Organic Chemistry
Wiley., 2022(1), e202101265.
https://doi.org/10.1002/ejoc.202101265
Selaković Ž, Nikolić A, Ajdačić V, Opsenica I. Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products. in European Journal of Organic Chemistry. 2022;2022(1):e202101265.
doi:10.1002/ejoc.202101265 .
Selaković, Života, Nikolić, Andrea, Ajdačić, Vladimir, Opsenica, Igor, "Application of Transition Metal-Catalyzed Decarbonylation of Aldehydes in the Total Synthesis of Natural Products" in European Journal of Organic Chemistry, 2022, no. 1 (2022):e202101265,
https://doi.org/10.1002/ejoc.202101265 . .
5
2
1

The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta

Manojlović-Stojanoski, Milica; Borković-Mitić, Slavica; Nestorović, Nataša; Ristić, Nataša; Trifunović, Svetlana; Stevanović, Magdalena; Filipović, Nenad; Stojsavljević, Aleksandar; Pavlović, Slađan

(MDPI, 2022)

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5681
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta
VL  - 23
IS  - 21
SP  - 13068
DO  - 10.3390/ijms232113068
ER  - 
@article{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy, a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) (BSA-bovine serum albumin) and inorganic sodium selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy. Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta",
volume = "23",
number = "21",
pages = "13068",
doi = "10.3390/ijms232113068"
}
Manojlović-Stojanoski, M., Borković-Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. in International Journal of Molecular Sciences
MDPI., 23(21), 13068.
https://doi.org/10.3390/ijms232113068
Manojlović-Stojanoski M, Borković-Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068 .
Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The Effects of BSA-Stabilized Selenium Nanoparticles and Sodium Selenite Supplementation on the Structure, Oxidative Stress Parameters and Selenium Redox Biology in Rat Placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 . .

Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M

Mitić, Bojan M.; Borković-Mitić, Slavica; Stojsavljević, Aleksandar; Stojanović, Dalibor; Pavlović, Slađan; Vasiljević, Ljubica; Ristić, Nataša

(Belgrade : Serbian Biological Society, 2022)

TY  - DATA
AU  - Mitić, Bojan M.
AU  - Borković-Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Vasiljević, Ljubica
AU  - Ristić, Nataša
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5673
AB  - Three centipede species (Clinopodes flavidus, Cryptops anomalans and Eupolybothrus transsylvanicus) were usedas bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metalsMn, Co, Ni, Cu, Zn, Rb, Sr, Cd, Tl, Pb and U and metalloids As, Se) in whole animals and soil were measured by inductivelycoupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed specieswere observed, both in response to the sites and between species. In most cases, the trace element concentrations werehigher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E.transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus wasa good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient inaccumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E.transsylvanicus, which accumulated Cd according to the high bioaccumulation factor (BAF) values. We conclude that thecentipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure.Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the routeof exposure.
PB  - Belgrade : Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M
VL  - 74
IS  - 3
SP  - 207
EP  - 215
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5673
ER  - 
@misc{
author = "Mitić, Bojan M. and Borković-Mitić, Slavica and Stojsavljević, Aleksandar and Stojanović, Dalibor and Pavlović, Slađan and Vasiljević, Ljubica and Ristić, Nataša",
year = "2022",
abstract = "Three centipede species (Clinopodes flavidus, Cryptops anomalans and Eupolybothrus transsylvanicus) were usedas bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metalsMn, Co, Ni, Cu, Zn, Rb, Sr, Cd, Tl, Pb and U and metalloids As, Se) in whole animals and soil were measured by inductivelycoupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed specieswere observed, both in response to the sites and between species. In most cases, the trace element concentrations werehigher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E.transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus wasa good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient inaccumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E.transsylvanicus, which accumulated Cd according to the high bioaccumulation factor (BAF) values. We conclude that thecentipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure.Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the routeof exposure.",
publisher = "Belgrade : Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M",
volume = "74",
number = "3",
pages = "207-215",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5673"
}
Mitić, B. M., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M. in Archives of Biological Sciences
Belgrade : Serbian Biological Society., 74(3), 207-215.
https://hdl.handle.net/21.15107/rcub_cherry_5673
Mitić BM, Borković-Mitić S, Stojsavljević A, Stojanović D, Pavlović S, Vasiljević L, Ristić N. Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M. in Archives of Biological Sciences. 2022;74(3):207-215.
https://hdl.handle.net/21.15107/rcub_cherry_5673 .
Mitić, Bojan M., Borković-Mitić, Slavica, Stojsavljević, Aleksandar, Stojanović, Dalibor, Pavlović, Slađan, Vasiljević, Ljubica, Ristić, Nataša, "Supplementary material for: Mitić, B., Borković-Mitić, S., Stojsavljević, A., Stojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences Belgrade : Serbian Biological Society., 74(3), 207-215. https://doi.org/10.2298/ABS220514019M" in Archives of Biological Sciences, 74, no. 3 (2022):207-215,
https://hdl.handle.net/21.15107/rcub_cherry_5673 .