TY  - DATA
AU  - Mutić, Tamara
AU  - Stanić-Vučinić, Dragana
AU  - Mutić, Jelena
AU  - Ilić, Miloš
AU  - Jovanović, Vesna
AU  - Aćimović, Jelena
AU  - Anđelković, Boban
AU  - Krishna de Guzman, Maria
AU  - Anđelković, Mirjana
AU  - Turkalj, Mirjana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6484
AB  - The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.
T1  - Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6484
ER  - 

@misc{
author = "Mutić, Tamara and Stanić-Vučinić, Dragana and Mutić, Jelena and Ilić, Miloš and Jovanović, Vesna and Aćimović, Jelena and Anđelković, Boban and Krishna de Guzman, Maria and Anđelković, Mirjana and Turkalj, Mirjana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.",
title = "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6484"
}

Mutić, T., Stanić-Vučinić, D., Mutić, J., Ilić, M., Jovanović, V., Aćimović, J., Anđelković, B., Krishna de Guzman, M., Anđelković, M., Turkalj, M.,& Ćirković-Veličković, T.. (2024). Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. .
https://hdl.handle.net/21.15107/rcub_cherry_6484

Mutić T, Stanić-Vučinić D, Mutić J, Ilić M, Jovanović V, Aćimović J, Anđelković B, Krishna de Guzman M, Anđelković M, Turkalj M, Ćirković-Veličković T. Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6484 .

Mutić, Tamara, Stanić-Vučinić, Dragana, Mutić, Jelena, Ilić, Miloš, Jovanović, Vesna, Aćimović, Jelena, Anđelković, Boban, Krishna de Guzman, Maria, Anđelković, Mirjana, Turkalj, Mirjana, Ćirković-Veličković, Tanja, "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6484 .

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6510
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
VL  - 267
SP  - 131564
DO  - 10.1016/j.ijbiomac.2024.131564
ER  - 

@article{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and Aćimović, Jelena and de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
volume = "267",
pages = "131564",
doi = "10.1016/j.ijbiomac.2024.131564"
}

Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., Aćimović, J., de Guzman, M. K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. in International Journal of Biological Macromolecules
Elsevier., 267, 131564.
https://doi.org/10.1016/j.ijbiomac.2024.131564

Gligorijević N, Lujić T, Mutić T, Vasović T, Aćimović J, de Guzman MK, Stanić-Vučinić D, Ćirković-Veličković T. Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. in International Journal of Biological Macromolecules. 2024;267:131564.
doi:10.1016/j.ijbiomac.2024.131564 .

Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, Aćimović, Jelena, de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" in International Journal of Biological Macromolecules, 267 (2024):131564,
https://doi.org/10.1016/j.ijbiomac.2024.131564 . .

TY  - DATA
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6469
AB  - Research data for the unpublished paper: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. Densitometry analysis of ovalbumin band intensity after digestion in the presence of microplastics on SDS-PAGE gels.
T1  - Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6469
ER  - 

@misc{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and Aćimović, Jelena and de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Research data for the unpublished paper: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. Densitometry analysis of ovalbumin band intensity after digestion in the presence of microplastics on SDS-PAGE gels.",
title = "Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6469"
}

Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., Aćimović, J., de Guzman, M. K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. .
https://hdl.handle.net/21.15107/rcub_cherry_6469

Gligorijević N, Lujić T, Mutić T, Vasović T, Aćimović J, de Guzman MK, Stanić-Vučinić D, Ćirković-Veličković T. Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6469 .

Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, Aćimović, Jelena, de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6469 .

TY  - DATA
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - de Guzman, Maria Krishna
AU  - Aćimović, Jelena
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6465
AB  - Data used for the analysis of pepsin interaction with polystyrene microplastic of 10 and 100 µm in size. Binding isotherms data were used for the construction of binding isotherms from which binding between pepsin and polystyrene microplastic was described. Structural features of pepsin in the presence of polystyrene were analysed by CD spectrometry and spectrofluorimetry.
T1  - Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6465
ER  - 

@misc{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and de Guzman, Maria Krishna and Aćimović, Jelena and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Data used for the analysis of pepsin interaction with polystyrene microplastic of 10 and 100 µm in size. Binding isotherms data were used for the construction of binding isotherms from which binding between pepsin and polystyrene microplastic was described. Structural features of pepsin in the presence of polystyrene were analysed by CD spectrometry and spectrofluorimetry.",
title = "Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6465"
}

Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., de Guzman, M. K., Aćimović, J., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. .
https://hdl.handle.net/21.15107/rcub_cherry_6465

Gligorijević N, Lujić T, Mutić T, Vasović T, de Guzman MK, Aćimović J, Stanić-Vučinić D, Ćirković-Veličković T. Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6465 .

Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, de Guzman, Maria Krishna, Aćimović, Jelena, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6465 .

TY  - DATA
AU  - Mutić, Tamara
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6483
AB  - Overview of full chemical and morphological characterization of  MPs in clams, mussels and shrimps from different regions
T1  - Research data no. 2 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6483
ER  - 

@misc{
author = "Mutić, Tamara",
year = "2024",
abstract = "Overview of full chemical and morphological characterization of  MPs in clams, mussels and shrimps from different regions",
title = "Research data no. 2 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6483"
}

Mutić, T.. (2024). Research data no. 2 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. .
https://hdl.handle.net/21.15107/rcub_cherry_6483

Mutić T. Research data no. 2 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6483 .

Mutić, Tamara, "Research data no. 2 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6483 .

TY  - CONF
AU  - Mutić, Tamara
AU  - Ilić, Miloš
AU  - Mutić, Jelena
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6520
AB  - Microplastics (MPs) have been considered a new type of pollutant in the marine environment and have attracted widespread attention worldwide in recent years. Plastic
particles with particle size less than 5 mm are defined as MPs [1].
Mussels were used as an indicator of marine environments contamination in biomonitoring programs [2]. The aim of this study was to determine the abundance of MPs in Korean or hard-shelled mussel, one of the most economically and ecologically important bivalves in South Korea, collected from seafood market intended for human consumption. After extensive removal of biological material by alkaline, enzymatic and oxidative digestion, MPs were characterized by micro-FTIR spectroscopy, which is a proven method for the identification of all types of polymers and enables reliable differentiation between substances of natural and synthetic origin. In total, we found 0.53 MPs per individual which is less than 0.3 MPs per gram of soft tissue. Major types of isolated MPs with their size and shape are shown in Figure 1.
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences
T1  - Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy
SP  - 86
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6520
ER  - 

@conference{
author = "Mutić, Tamara and Ilić, Miloš and Mutić, Jelena and Ćirković Veličković, Tanja",
year = "2023",
abstract = "Microplastics (MPs) have been considered a new type of pollutant in the marine environment and have attracted widespread attention worldwide in recent years. Plastic
particles with particle size less than 5 mm are defined as MPs [1].
Mussels were used as an indicator of marine environments contamination in biomonitoring programs [2]. The aim of this study was to determine the abundance of MPs in Korean or hard-shelled mussel, one of the most economically and ecologically important bivalves in South Korea, collected from seafood market intended for human consumption. After extensive removal of biological material by alkaline, enzymatic and oxidative digestion, MPs were characterized by micro-FTIR spectroscopy, which is a proven method for the identification of all types of polymers and enables reliable differentiation between substances of natural and synthetic origin. In total, we found 0.53 MPs per individual which is less than 0.3 MPs per gram of soft tissue. Major types of isolated MPs with their size and shape are shown in Figure 1.",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences",
title = "Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6520"
}

Mutić, T., Ilić, M., Mutić, J.,& Ćirković Veličković, T.. (2023). Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences
Belgrade : Serbian Chemical Society., 86-86.
https://hdl.handle.net/21.15107/rcub_cherry_6520

Mutić T, Ilić M, Mutić J, Ćirković Veličković T. Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences. 2023;:86-86.
https://hdl.handle.net/21.15107/rcub_cherry_6520 .

Mutić, Tamara, Ilić, Miloš, Mutić, Jelena, Ćirković Veličković, Tanja, "Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences (2023):86-86,
https://hdl.handle.net/21.15107/rcub_cherry_6520 .

TY  - CONF
AU  - Mutić, Tamara
AU  - Anđelković, Boban
AU  - Stanić-Vučinić, Dragana
AU  - Turkalj, Mirjana
AU  - Ilić, Miloš
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6519
AB  - Seafood has been widely recognized as an integral part of a balanced and healthy diet, thanks to its high nutritional value and associated multiple health benefits. Seafood contamination with microplastics (MPs) have recently been highlighted as an emerging concern for global food security.1
According to definition, polymer particles with a diameter of less than 5 mm are referred to as microplastics particles (MPs)2. Most bivalves, as well as mussels, are filter feeders and can accumulate particles in their bodies. By consuming seafood, MP particles can be included in the human food chain. Most of the studies analyzed MPs content in environmental samples, instead of point-of-sales samples.
The aim of this study was to determine the abundance of microplastics in mussels collected from Croatia seafood market intended for human consumption. 5 clean laboratory criteria (100% cotton lab coat, clean glassware, filtered solutions, rinsed the outside of animals before dissection, and limited air exposure of samples) were fulfilled by our study. Digestion and manipulation of the sample was conducted in a clean hood. Procedural controls were included for each measurement. After extensive removal of biological material by alkaline, enzymatic and oxidation digestion, MPs were characterized by micro-Fourier-transform infrared spectroscopy (Micro-FTIR). It is a proven method for the identification of all types of polymers and allows a reliable differentiation between substances of natural and synthetic origin. Our approach included one individual per sample for micro-FTIR analysis, no sub-grouping of the sample for chemical characterization (100% of particles isolated from each sample have been analyzed by micro- FTIR) and manual confirmation of all recorded spectra with more than 60% match to the polymer material in the spectral library (Thermo-Fisher; using the OMNIC PictaTM software). Soft tissue from inside the shell was not extensively washed to allow estimation of MPs intake during consumption.
Almost half of total samples (sample size=27) did not contain microplastic particles (52%). The highest number of particles per individual was 4 (Fig. 1). In total, we found 23 MPs particles in 27 individuals or 0.85 MPs per individual, range 0-4, and less than 0.3 MPs/g of soft tissue. In this research, major types of isolated MPs were polystyrene
(PS), poly(ethylene terephthalate) (PET), polypropylene (PP) and polyethylene (PE), shown in Table 1. PE and PS are the most popular plastic materials used in consumer products and they have shorter service lives than other types of plastics.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
T1  - Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy
SP  - 281
EP  - 281
ER  - 

@conference{
author = "Mutić, Tamara and Anđelković, Boban and Stanić-Vučinić, Dragana and Turkalj, Mirjana and Ilić, Miloš and Ćirković Veličković, Tanja",
year = "2023",
abstract = "Seafood has been widely recognized as an integral part of a balanced and healthy diet, thanks to its high nutritional value and associated multiple health benefits. Seafood contamination with microplastics (MPs) have recently been highlighted as an emerging concern for global food security.1
According to definition, polymer particles with a diameter of less than 5 mm are referred to as microplastics particles (MPs)2. Most bivalves, as well as mussels, are filter feeders and can accumulate particles in their bodies. By consuming seafood, MP particles can be included in the human food chain. Most of the studies analyzed MPs content in environmental samples, instead of point-of-sales samples.
The aim of this study was to determine the abundance of microplastics in mussels collected from Croatia seafood market intended for human consumption. 5 clean laboratory criteria (100% cotton lab coat, clean glassware, filtered solutions, rinsed the outside of animals before dissection, and limited air exposure of samples) were fulfilled by our study. Digestion and manipulation of the sample was conducted in a clean hood. Procedural controls were included for each measurement. After extensive removal of biological material by alkaline, enzymatic and oxidation digestion, MPs were characterized by micro-Fourier-transform infrared spectroscopy (Micro-FTIR). It is a proven method for the identification of all types of polymers and allows a reliable differentiation between substances of natural and synthetic origin. Our approach included one individual per sample for micro-FTIR analysis, no sub-grouping of the sample for chemical characterization (100% of particles isolated from each sample have been analyzed by micro- FTIR) and manual confirmation of all recorded spectra with more than 60% match to the polymer material in the spectral library (Thermo-Fisher; using the OMNIC PictaTM software). Soft tissue from inside the shell was not extensively washed to allow estimation of MPs intake during consumption.
Almost half of total samples (sample size=27) did not contain microplastic particles (52%). The highest number of particles per individual was 4 (Fig. 1). In total, we found 23 MPs particles in 27 individuals or 0.85 MPs per individual, range 0-4, and less than 0.3 MPs/g of soft tissue. In this research, major types of isolated MPs were polystyrene
(PS), poly(ethylene terephthalate) (PET), polypropylene (PP) and polyethylene (PE), shown in Table 1. PE and PS are the most popular plastic materials used in consumer products and they have shorter service lives than other types of plastics.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023",
title = "Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy",
pages = "281-281"
}

Mutić, T., Anđelković, B., Stanić-Vučinić, D., Turkalj, M., Ilić, M.,& Ćirković Veličković, T.. (2023). Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
Belgrade : Serbian Chemical Society., 281-281.

Mutić T, Anđelković B, Stanić-Vučinić D, Turkalj M, Ilić M, Ćirković Veličković T. Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023. 2023;:281-281..

Mutić, Tamara, Anđelković, Boban, Stanić-Vučinić, Dragana, Turkalj, Mirjana, Ilić, Miloš, Ćirković Veličković, Tanja, "Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy" in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023 (2023):281-281.

TY  - CONF
AU  - Ćirković-Veličković, Tanja
AU  - Stanić-Vučinić, Dragana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6522
AB  - Human exposure to nano- and microplastics (NMPs) is a reality, and contamination of food with NMPs
has become an issue worldwide. Due to their small size and pervasive nature, NMPs may enter the
human body through various routes, ingestion being the most relevant route for food and beverages
contaminated with NMPs. The presence of NMPs in the GIT allows them to reach different parts of the
body, where they may have both physical and chemical effects.
GIT is also the first point of contact of food allergens and NMPs. To clarify effects of food allergens
adsorption and bio-corona formation on NMPs, we have examined binding and structural changes of
several allergenic food proteins (ovalbumine, beta-lactoglobuline, tropomyosine) to the polystyrene,
polypropylene, and polyethylene terephthalate microplastics. Our results show high binding affinity of
food allergens to the plastic particles and structural and functional changes in the allergenic food
proteins bound in soft and hard corona. Furthermore, presence of NMPs in the digestive fluid may
influence digestion of allergenic food proteins. Our study on the pepsin digestion of milk proteins in the
presence of PS showed that cow’s milk proteins (caseins) and their larger fragments preferentially bind
to the hard corona of PS-MPs during the gastric digestion of milk leading to prolonged survival of larger
peptides (particularly of αS2-casein) in the simulated gastric fluid. In future studies, it will be particularly
relevant to further study impact of nanoplastics on allergenic proteins digestion and bioavailability.
Larger surface area of nanoplastics in comparison to microplastics may provide more anchoring points
for proteins and digestive enzymes to tightly bind in the hard corona, causing structural changes and
possibly impacting digestive enzymes’ function. In the future, particular attention should be given to
infant, elderly, and groups already showing digestion impairment, as most of the studies conducted so
far consider only healthy adult digestion. However, data so far obtained at the molecular level suggest
adsorption and structural changes in allergenic food proteins, particularly in bio-corona around NMPs
and impairment of function of digestive enzymes. Studies are warranted on the impact of NMPs/allergen
co-exposure on allergenicity and sensitizing potential of allergenic foods contaminated with NMP to fully
understand possible impact of NMPs on development and severity of food allergies.
PB  - TNO and UMC Utrecht, the Netherlands
C3  - The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023
T1  - Food allergen bio-corona on microplastics
SP  - 17
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6522
ER  - 

@conference{
author = "Ćirković-Veličković, Tanja and Stanić-Vučinić, Dragana",
year = "2023",
abstract = "Human exposure to nano- and microplastics (NMPs) is a reality, and contamination of food with NMPs
has become an issue worldwide. Due to their small size and pervasive nature, NMPs may enter the
human body through various routes, ingestion being the most relevant route for food and beverages
contaminated with NMPs. The presence of NMPs in the GIT allows them to reach different parts of the
body, where they may have both physical and chemical effects.
GIT is also the first point of contact of food allergens and NMPs. To clarify effects of food allergens
adsorption and bio-corona formation on NMPs, we have examined binding and structural changes of
several allergenic food proteins (ovalbumine, beta-lactoglobuline, tropomyosine) to the polystyrene,
polypropylene, and polyethylene terephthalate microplastics. Our results show high binding affinity of
food allergens to the plastic particles and structural and functional changes in the allergenic food
proteins bound in soft and hard corona. Furthermore, presence of NMPs in the digestive fluid may
influence digestion of allergenic food proteins. Our study on the pepsin digestion of milk proteins in the
presence of PS showed that cow’s milk proteins (caseins) and their larger fragments preferentially bind
to the hard corona of PS-MPs during the gastric digestion of milk leading to prolonged survival of larger
peptides (particularly of αS2-casein) in the simulated gastric fluid. In future studies, it will be particularly
relevant to further study impact of nanoplastics on allergenic proteins digestion and bioavailability.
Larger surface area of nanoplastics in comparison to microplastics may provide more anchoring points
for proteins and digestive enzymes to tightly bind in the hard corona, causing structural changes and
possibly impacting digestive enzymes’ function. In the future, particular attention should be given to
infant, elderly, and groups already showing digestion impairment, as most of the studies conducted so
far consider only healthy adult digestion. However, data so far obtained at the molecular level suggest
adsorption and structural changes in allergenic food proteins, particularly in bio-corona around NMPs
and impairment of function of digestive enzymes. Studies are warranted on the impact of NMPs/allergen
co-exposure on allergenicity and sensitizing potential of allergenic foods contaminated with NMP to fully
understand possible impact of NMPs on development and severity of food allergies.",
publisher = "TNO and UMC Utrecht, the Netherlands",
journal = "The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023",
title = "Food allergen bio-corona on microplastics",
pages = "17-17",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6522"
}

Ćirković-Veličković, T.,& Stanić-Vučinić, D.. (2023). Food allergen bio-corona on microplastics. in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023
TNO and UMC Utrecht, the Netherlands., 17-17.
https://hdl.handle.net/21.15107/rcub_cherry_6522

Ćirković-Veličković T, Stanić-Vučinić D. Food allergen bio-corona on microplastics. in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023. 2023;:17-17.
https://hdl.handle.net/21.15107/rcub_cherry_6522 .

Ćirković-Veličković, Tanja, Stanić-Vučinić, Dragana, "Food allergen bio-corona on microplastics" in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023 (2023):17-17,
https://hdl.handle.net/21.15107/rcub_cherry_6522 .

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Postić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - Van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6460
AB  - Percentage of hemolysis of RBC in the presence of washed PET NPs and
unwashed NPs (with SDS) determined by incubation of human red blood cells (RBCs) from
three healthy donors. Output from the  Shimadzu UV/ViS 1800 (Kyoto, Japan) nspectrometer - raw spectral data and the calculation of percentages of hemolysis (procesesd data).
PB  - MDPI
T2  - Polymers
T1  - Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6460
ER  - 

@misc{
author = "Đapović, Milica and Apostolović, Danijela and Postić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and Van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Percentage of hemolysis of RBC in the presence of washed PET NPs and
unwashed NPs (with SDS) determined by incubation of human red blood cells (RBCs) from
three healthy donors. Output from the  Shimadzu UV/ViS 1800 (Kyoto, Japan) nspectrometer - raw spectral data and the calculation of percentages of hemolysis (procesesd data).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6460"
}

Đapović, M., Apostolović, D., Postić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., Van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6460

Đapović M, Apostolović D, Postić V, Lujić T, Jovanović V, Stanić-Vučinić D, Van Hage M, Maslak V, Ćirković-Veličković T. Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6460 .

Đapović, Milica, Apostolović, Danijela, Postić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, Van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6460 .

TY  - CONF
AU  - Mutić, Tamara
AU  - Anđelković, Boban
AU  - Ilić, Miloš
AU  - Mutić, Jelena
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6513
AB  - Tokom protekle decenije došlo je do velikog naučnog i javnog interesovanja za uticaj plastike na životnu sredinu, žive organizme, uključujući ljude i živi svet u morskim ekosistemima. [1]
 Izdvajanje plastičnih čestica iz uzorka radi njihove detekcije i dalje karakterizacije predstavlja veliki izazov jer su često pomešne sa velikim količinama mineralnih i biogenih organskih supstanci. Primenjeni protokoli treba da ostave intaktne sintetičke polimere prvenstveno u pogledu njihove hemijske strukture, a zatim i u pogledu njihove težine, zapremine, oblika i, ako je potrebno, boje. Očuvanje hemijske strukture ili površinske strukture i morfologije polimera u velikoj meri zavisi od odabira adekvatne metode digestije. [2]
 U ovom istraživanju ekstrakcija mikroplastike (MP) iz morskih organizama se zasnivala na višestepenoj digestiji, kao i na kombinovanju hemijske i enzimske digestije. Prvi stepen je hemijska digestija, koja podrazumeva upotrebu jakih alkalnih uslova sredine (KOH koncentracije 10 %), a drugi stepen ekstrakcije je digestija sa dva enzima, prvo pepsinom i zatim pankreatinom. Poslednji korak koji je od velike važnosti za dalja spekralna ispitivanja je bila upotreba oksidativnih reagenasa, odnosno vodonik peroksida, koncentracije 15 %.
 Analizirani su standardi čestica MP vrste PP veličine 500 μm, PVC veličine 120 μm, PS veličine 500 μm i PET veličine 120 μm. Čestice su dodate pre digestije (spike), a nakon što su prošle digestiju po optimizovanom protokolu su analizirane primenom μFTIR- spektrometra.
 Kod čestica PP, PVC i PS ne dolazi do promene strukture i nalažene su čestice sa velikim procentom podudaranja nakon digestije. Rezultati prikazani u Tabeli 1 pokazuju da se određeni tipovi plastike izloženi hemijskoj i enzimskoj digestiji, kao i eventualnom taloženju biološkog materijala, identifikuju sa manjim stepenom podudaranja sa bazama podataka, nego pre tretmana. I pored toga, procenat podudaranja sa bazama je iznosio za PP do 90 %, za PVC do 94 %, za PS do 68 % i za PET do 70 %.
Podaci prikazani na slici 1 predstavljaju šematski prikaz toka analize koja uključuje višestepenu analizu uzoraka, prebacivanje digestovane materije sa mikroplastičnim česticama i karakterizaciju μFTIR spektrometrom.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023
T1  - Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy
T1  - Izolovanje mikroplastičnih čestica iz uzoraka poreklom iz morskog ekosistema
i karakterizacija primenom µFTIR spektroskopije
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6513
ER  - 

@conference{
author = "Mutić, Tamara and Anđelković, Boban and Ilić, Miloš and Mutić, Jelena and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Tokom protekle decenije došlo je do velikog naučnog i javnog interesovanja za uticaj plastike na životnu sredinu, žive organizme, uključujući ljude i živi svet u morskim ekosistemima. [1]
 Izdvajanje plastičnih čestica iz uzorka radi njihove detekcije i dalje karakterizacije predstavlja veliki izazov jer su često pomešne sa velikim količinama mineralnih i biogenih organskih supstanci. Primenjeni protokoli treba da ostave intaktne sintetičke polimere prvenstveno u pogledu njihove hemijske strukture, a zatim i u pogledu njihove težine, zapremine, oblika i, ako je potrebno, boje. Očuvanje hemijske strukture ili površinske strukture i morfologije polimera u velikoj meri zavisi od odabira adekvatne metode digestije. [2]
 U ovom istraživanju ekstrakcija mikroplastike (MP) iz morskih organizama se zasnivala na višestepenoj digestiji, kao i na kombinovanju hemijske i enzimske digestije. Prvi stepen je hemijska digestija, koja podrazumeva upotrebu jakih alkalnih uslova sredine (KOH koncentracije 10 %), a drugi stepen ekstrakcije je digestija sa dva enzima, prvo pepsinom i zatim pankreatinom. Poslednji korak koji je od velike važnosti za dalja spekralna ispitivanja je bila upotreba oksidativnih reagenasa, odnosno vodonik peroksida, koncentracije 15 %.
 Analizirani su standardi čestica MP vrste PP veličine 500 μm, PVC veličine 120 μm, PS veličine 500 μm i PET veličine 120 μm. Čestice su dodate pre digestije (spike), a nakon što su prošle digestiju po optimizovanom protokolu su analizirane primenom μFTIR- spektrometra.
 Kod čestica PP, PVC i PS ne dolazi do promene strukture i nalažene su čestice sa velikim procentom podudaranja nakon digestije. Rezultati prikazani u Tabeli 1 pokazuju da se određeni tipovi plastike izloženi hemijskoj i enzimskoj digestiji, kao i eventualnom taloženju biološkog materijala, identifikuju sa manjim stepenom podudaranja sa bazama podataka, nego pre tretmana. I pored toga, procenat podudaranja sa bazama je iznosio za PP do 90 %, za PVC do 94 %, za PS do 68 % i za PET do 70 %.
Podaci prikazani na slici 1 predstavljaju šematski prikaz toka analize koja uključuje višestepenu analizu uzoraka, prebacivanje digestovane materije sa mikroplastičnim česticama i karakterizaciju μFTIR spektrometrom.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023",
title = "Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy, Izolovanje mikroplastičnih čestica iz uzoraka poreklom iz morskog ekosistema
i karakterizacija primenom µFTIR spektroskopije",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6513"
}

Mutić, T., Anđelković, B., Ilić, M., Mutić, J.,& Ćirković-Veličković, T.. (2023). Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023
Beograd : Srpsko hemijsko društvo., 89-90.
https://hdl.handle.net/21.15107/rcub_cherry_6513

Mutić T, Anđelković B, Ilić M, Mutić J, Ćirković-Veličković T. Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023. 2023;:89-90.
https://hdl.handle.net/21.15107/rcub_cherry_6513 .

Mutić, Tamara, Anđelković, Boban, Ilić, Miloš, Mutić, Jelena, Ćirković-Veličković, Tanja, "Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy" in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023 (2023):89-90,
https://hdl.handle.net/21.15107/rcub_cherry_6513 .

TY  - JOUR
AU  - Kaseke, Tafadzwa
AU  - Lujic, Tamara
AU  - Cirkovic Velickovic, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6512
AB  - : The ongoing use of plastic polymers to manufacture food packaging has raised concerns
about the presence of nano- and microplastics (NMPs) in a variety of foods. This review provides the
most recent data on NMPs’ migration from plastic packaging into dairy products. Also discussed
are the possible effects of NMPs on nutrient digestion, absorption, and metabolism. Different kinds
of dairy products, including skimmed milk, whole liquid milk, powder milk, and infant formula
milk, have been found to contain NMPs of various sizes, shapes, and concentrations. NMPs may
interact with proteins, carbohydrates, and fats and have a detrimental impact on how well these
nutrients are digested and absorbed by the body. The presence of NMPs in the gastrointestinal
tract may impact how lipids, proteins, glucose, iron, and energy are metabolized, increasing the
risk of developing various health conditions. In addition to NMPs, plastic oligomers released from
food packaging material have been found to migrate to various foods and food simulants, though
information regarding their effect on human health is limited. Viewpoints on potential directions
for future studies on NMPs and their impact on nutrient digestion, absorption, and health are also
presented in this review.
PB  - MDPI
T2  - Foods
T1  - Nano- and Microplastics Migration from Plastic Food  Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism
VL  - 12
IS  - 16
SP  - 3043
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6512
ER  - 

@article{
author = "Kaseke, Tafadzwa and Lujic, Tamara and Cirkovic Velickovic, Tanja",
year = "2023",
abstract = ": The ongoing use of plastic polymers to manufacture food packaging has raised concerns
about the presence of nano- and microplastics (NMPs) in a variety of foods. This review provides the
most recent data on NMPs’ migration from plastic packaging into dairy products. Also discussed
are the possible effects of NMPs on nutrient digestion, absorption, and metabolism. Different kinds
of dairy products, including skimmed milk, whole liquid milk, powder milk, and infant formula
milk, have been found to contain NMPs of various sizes, shapes, and concentrations. NMPs may
interact with proteins, carbohydrates, and fats and have a detrimental impact on how well these
nutrients are digested and absorbed by the body. The presence of NMPs in the gastrointestinal
tract may impact how lipids, proteins, glucose, iron, and energy are metabolized, increasing the
risk of developing various health conditions. In addition to NMPs, plastic oligomers released from
food packaging material have been found to migrate to various foods and food simulants, though
information regarding their effect on human health is limited. Viewpoints on potential directions
for future studies on NMPs and their impact on nutrient digestion, absorption, and health are also
presented in this review.",
publisher = "MDPI",
journal = "Foods",
title = "Nano- and Microplastics Migration from Plastic Food  Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism",
volume = "12",
number = "16",
pages = "3043",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6512"
}

Kaseke, T., Lujic, T.,& Cirkovic Velickovic, T.. (2023). Nano- and Microplastics Migration from Plastic Food  Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism. in Foods
MDPI., 12(16), 3043.
https://hdl.handle.net/21.15107/rcub_cherry_6512

Kaseke T, Lujic T, Cirkovic Velickovic T. Nano- and Microplastics Migration from Plastic Food  Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism. in Foods. 2023;12(16):3043.
https://hdl.handle.net/21.15107/rcub_cherry_6512 .

Kaseke, Tafadzwa, Lujic, Tamara, Cirkovic Velickovic, Tanja, "Nano- and Microplastics Migration from Plastic Food  Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism" in Foods, 12, no. 16 (2023):3043,
https://hdl.handle.net/21.15107/rcub_cherry_6512 .

TY  - DATA
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Gligorijević, Nikola
AU  - Wimmer, Lukas
AU  - Gasparyan, Manvel
AU  - Lujić, Tamara
AU  - Vasović, Tamara
AU  - Dailey, Lea Ann
AU  - Van Haute, Sam
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6463
AB  - The analysis of structural changes of pepsin in the presence of polystyrene microplastic of 10 and 100 µm in size in simulated gastric fluid. Data obtained from J-815 CD spectropolarimeter, and further analyzed by Origin and Excel software.
T2  - Environmental Pollution
T1  - Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6463
ER  - 

@misc{
author = "de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Gligorijević, Nikola and Wimmer, Lukas and Gasparyan, Manvel and Lujić, Tamara and Vasović, Tamara and Dailey, Lea Ann and Van Haute, Sam and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The analysis of structural changes of pepsin in the presence of polystyrene microplastic of 10 and 100 µm in size in simulated gastric fluid. Data obtained from J-815 CD spectropolarimeter, and further analyzed by Origin and Excel software.",
journal = "Environmental Pollution",
title = "Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6463"
}

de Guzman, M. K., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282. in Environmental Pollution.
https://hdl.handle.net/21.15107/rcub_cherry_6463

de Guzman MK, Stanić-Vučinić D, Gligorijević N, Wimmer L, Gasparyan M, Lujić T, Vasović T, Dailey LA, Van Haute S, Ćirković-Veličković T. Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282. in Environmental Pollution. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6463 .

de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Gligorijević, Nikola, Wimmer, Lukas, Gasparyan, Manvel, Lujić, Tamara, Vasović, Tamara, Dailey, Lea Ann, Van Haute, Sam, Ćirković-Veličković, Tanja, "Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282" in Environmental Pollution (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6463 .

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6464
AB  - Determination of hydrodynamic diameter of PET NPs washed and dispersed in water, BSA (0.05%), and SDS (0.1%), and NPs unwashed and dispersed in water.  Zeta potential, mobility, and conductivity determination in PET NPs at different stages of purification. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).
PB  - MDPI
T2  - Polymers
T1  - Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6464
ER  - 

@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Determination of hydrodynamic diameter of PET NPs washed and dispersed in water, BSA (0.05%), and SDS (0.1%), and NPs unwashed and dispersed in water.  Zeta potential, mobility, and conductivity determination in PET NPs at different stages of purification. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6464"
}

Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6464

Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6464 .

Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6464 .

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6467
AB  - The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. 1H NMR spectra of the NPs preparation before and during all the washing steps. Determination of SDS Level in Corona of PET NPs by 1H NMR. Output from the Varian/Agilent NMR 400 MHz. NMR spectra were processed in Mnova software.
PB  - MDPI
T2  - Polymers
T1  - Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6467
ER  - 

@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. 1H NMR spectra of the NPs preparation before and during all the washing steps. Determination of SDS Level in Corona of PET NPs by 1H NMR. Output from the Varian/Agilent NMR 400 MHz. NMR spectra were processed in Mnova software.",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6467"
}

Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6467

Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6467 .

Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6467 .

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6468
AB  - Size distributions (by intensity percentage) of NPs washed suspended in different dispersants, water,BSA (0.05%),SDS (0.1 %). Distributions data from different combined fractions: from NP-10 to NP-30; from NP-40 to NP-60 and from NP-70 to NP-90. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).
PB  - MDPI
T2  - Polymers
T1  - Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6468
ER  - 

@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Size distributions (by intensity percentage) of NPs washed suspended in different dispersants, water,BSA (0.05%),SDS (0.1 %). Distributions data from different combined fractions: from NP-10 to NP-30; from NP-40 to NP-60 and from NP-70 to NP-90. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6468"
}

Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6468

Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6468 .

Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6468 .

TY  - JOUR
AU  - Djapovic, Milica
AU  - Apostolović, Danijela
AU  - Postic, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6433
AB  - Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.
PB  - MDPI
T2  - Polymers
T1  - Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes
VL  - 15
IS  - 24
SP  - 4703
DO  - 10.3390/polym15244703
ER  - 

@article{
author = "Djapovic, Milica and Apostolović, Danijela and Postic, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.",
publisher = "MDPI",
journal = "Polymers",
title = "Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes",
volume = "15",
number = "24",
pages = "4703",
doi = "10.3390/polym15244703"
}

Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703

Djapovic M, Apostolović D, Postic V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers. 2023;15(24):4703.
doi:10.3390/polym15244703 .

Djapovic, Milica, Apostolović, Danijela, Postic, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes" in Polymers, 15, no. 24 (2023):4703,
https://doi.org/10.3390/polym15244703 . .

TY  - CONF
AU  - Jovanović, Vesna
AU  - Radomirović, Mirjana Ž.
AU  - Krstić-Ristivojević, Maja
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6023
AB  - In the last several decades, the trend of seafood consumption has significantly increased not only in the countries with a tradition of seafood consumption, but also in other ones [1]. The increase in the world's population and the awareness of healthy food, the globalization of markets, and the development of aquaculture are some of the factors that have led to this trend. The aquaculture of shellfish like clams, mussels, oysters and scallops has been very developed all around the world and the food products based on them have become part of the daily diet for many consumers. In addition, these food products are considered healthy food because of the high content of proteins and essential fatty acids, but their consumption may carry some risks of food allergy. Tropomyosin from shellfish (TPM) is the major allergen responsible for the development of anaphylaxis in persons with food allergy. The content of TPM in shellfish and its bioavailability from food products can have potential influence on the sensitization of consumers to TPM. It is known that food processing can change the bioavailability of food allergens [2]. The main goal of this study was the investigation of how processing and packaging of shellfish samples can affect the content of TPM in them.
For this study, clam Venerupis philippinarum was chosen as the species with the highest world aquaculture production [1]. After the purchasing of live clams, the animals were separated into 5 groups for the next treatments: fresh live (control group), freshly removed inner content was kept at +4°C for 3 days (three days` shelf-life), frozen in a plastic bag and kept at -20 °C during 7 days, marinated and kept in a glass jar at room temperature during 8 days and freshly boiled. After processing and packaging of samples, the total protein extracts were prepared in 10 mM sodium phosphate buffer pH 7.4 1M NaCl, 1 mM PMSF and the concentration of total proteins was determined by BCA method. The concentration of TPM in the total protein extracts was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) using in-house prepared clams` TPM standard. The content of TPM (μg) in the samples was expressed per mg of extracted soluble proteins, individual animal and grams of soft wet tissue.
The cooked samples have significantly higher TPM content expressed per gram of soft wet tissue compared to all other treatments. Food processing such as freezing, marinating, or extending the shelf-life at 4°C by 3 days has very little effect on the change in TPM content per gram of soft wet tissue compared to the fresh samples. The processing of clams, like cooking or marinating, caused the content of total soluble extracted proteins to be three to four times lower compared to the other three treatments. In these samples the obtained ratio of the total TPM/ total soluble extracted proteins ratio was the highest. This result can be explained by the fact that TPM is thermostable and stays soluble after cooking, while other proteins become insoluble because of denaturation. The lower ratio of TPM/ total soluble extracted proteins was found in marinated samples compared to the cooked samples. The lowest total tropomyosin/ total soluble extracted proteins ratio was found in 3 days’ shelf-life. Treatments like cooking, marinating and keeping the inner content of the shell at +4°C can significantly affect extractability of proteins, particularly affecting the ratio of major allergen TPM in the total protein extracts. Further studies are needed to examine bioaccessibility of TPM in different biologically relevant fluids (gastric/intestinal) and during digestion in relation to the processing conditions.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.
T1  - The effect of food processing and packaging of clams on the content of tropomyosin
SP  - 240
EP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6023
ER  - 

@conference{
author = "Jovanović, Vesna and Radomirović, Mirjana Ž. and Krstić-Ristivojević, Maja and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "In the last several decades, the trend of seafood consumption has significantly increased not only in the countries with a tradition of seafood consumption, but also in other ones [1]. The increase in the world's population and the awareness of healthy food, the globalization of markets, and the development of aquaculture are some of the factors that have led to this trend. The aquaculture of shellfish like clams, mussels, oysters and scallops has been very developed all around the world and the food products based on them have become part of the daily diet for many consumers. In addition, these food products are considered healthy food because of the high content of proteins and essential fatty acids, but their consumption may carry some risks of food allergy. Tropomyosin from shellfish (TPM) is the major allergen responsible for the development of anaphylaxis in persons with food allergy. The content of TPM in shellfish and its bioavailability from food products can have potential influence on the sensitization of consumers to TPM. It is known that food processing can change the bioavailability of food allergens [2]. The main goal of this study was the investigation of how processing and packaging of shellfish samples can affect the content of TPM in them.
For this study, clam Venerupis philippinarum was chosen as the species with the highest world aquaculture production [1]. After the purchasing of live clams, the animals were separated into 5 groups for the next treatments: fresh live (control group), freshly removed inner content was kept at +4°C for 3 days (three days` shelf-life), frozen in a plastic bag and kept at -20 °C during 7 days, marinated and kept in a glass jar at room temperature during 8 days and freshly boiled. After processing and packaging of samples, the total protein extracts were prepared in 10 mM sodium phosphate buffer pH 7.4 1M NaCl, 1 mM PMSF and the concentration of total proteins was determined by BCA method. The concentration of TPM in the total protein extracts was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) using in-house prepared clams` TPM standard. The content of TPM (μg) in the samples was expressed per mg of extracted soluble proteins, individual animal and grams of soft wet tissue.
The cooked samples have significantly higher TPM content expressed per gram of soft wet tissue compared to all other treatments. Food processing such as freezing, marinating, or extending the shelf-life at 4°C by 3 days has very little effect on the change in TPM content per gram of soft wet tissue compared to the fresh samples. The processing of clams, like cooking or marinating, caused the content of total soluble extracted proteins to be three to four times lower compared to the other three treatments. In these samples the obtained ratio of the total TPM/ total soluble extracted proteins ratio was the highest. This result can be explained by the fact that TPM is thermostable and stays soluble after cooking, while other proteins become insoluble because of denaturation. The lower ratio of TPM/ total soluble extracted proteins was found in marinated samples compared to the cooked samples. The lowest total tropomyosin/ total soluble extracted proteins ratio was found in 3 days’ shelf-life. Treatments like cooking, marinating and keeping the inner content of the shell at +4°C can significantly affect extractability of proteins, particularly affecting the ratio of major allergen TPM in the total protein extracts. Further studies are needed to examine bioaccessibility of TPM in different biologically relevant fluids (gastric/intestinal) and during digestion in relation to the processing conditions.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.",
title = "The effect of food processing and packaging of clams on the content of tropomyosin",
pages = "240-240",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6023"
}

Jovanović, V., Radomirović, M. Ž., Krstić-Ristivojević, M., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). The effect of food processing and packaging of clams on the content of tropomyosin. in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.
Beograd : Srpsko hemijsko društvo., 240-240.
https://hdl.handle.net/21.15107/rcub_cherry_6023

Jovanović V, Radomirović MŽ, Krstić-Ristivojević M, Stanić-Vučinić D, Ćirković-Veličković T. The effect of food processing and packaging of clams on the content of tropomyosin. in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.. 2023;:240-240.
https://hdl.handle.net/21.15107/rcub_cherry_6023 .

Jovanović, Vesna, Radomirović, Mirjana Ž., Krstić-Ristivojević, Maja, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "The effect of food processing and packaging of clams on the content of tropomyosin" in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023. (2023):240-240,
https://hdl.handle.net/21.15107/rcub_cherry_6023 .

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Jovanović, Vesna
AU  - Radomirović, Mirjana Ž.
AU  - Trifunović, Olga
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6024
AB  - In the last 50 years, the annual per capita consumption of seafood products worldwide has more than doubled, from almost 10 kg in 1960 to over 20 kg in 2014. Seafood protein is an essential part of the diet in many countries, particularly where total protein intake is low [1]. However, as defined by the European Community, fish, and shellfish tropomyosins (TPM) are major allergens and major causes of anaphylaxis [2]. The increasing prevalence of food allergies is consistent with the increasing pollution of soil and water with plastic particles. To investigate the potential link between increasing plastic pollution and increasing food allergy prevalence, we aim to develop methods for precise and accurate monitoring of allergens and plastic in real seafood samples.
TPM was isolated from shrimp (Litopenaeus vannamei), clams (Venerupis philippinarum), and mussels (Mytilus galloprovincialis). The obtained in-house TPM proteins from three different sources were resolved using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The concentration of TPM in seashell samples from different geographical origin was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with prior optimization of adequate TPM standard curve using commercial and non-commercial in-house prepared TPM standards.
TPM standards resolved via 2D-PAGE revealed the presence of two isoforms of shrimp and mussels TPM standard, one dominant and one less abundant isoform. Two isoforms from both seafood sources, shrimp and mussels, are slightly different in molecular weight and pI value. As for the TPM standard obtained from clams, the 2D electrophoregram showed possibly eight isoforms with small differences in mass and pI values. Furthermore, the presence of three dominant isoforms can be observed that differ slightly in molecular mass, while other isoforms also differ in pI value. The ELISA results, regarding TPM standard curve optimization, showed that in both the commercial shrimp TPM and in-house shrimp TPM standards, sigmoidal concentration dependence is present in a range of 50 to 0.05 ng/ml, using serial double dilutions. On the other hand, TPM standards isolated from mussels and clams show sigmoidal concentration dependence in the range of 45 to 0.044 μg/ml with using the identical combination of capture and detection antibodies and serial double dilutions. TPM concentrations in clams and mussel samples extrapolated from standard curves of commercial shrimp TPM standard and corresponding in-house TPM standards are presented in Table 1.
Differences in TPM concentration of the same sample using different TPM standards differ from 40 to 600 times, which strongly indicates that the right choice of TPM standard is a critical step for accurate and precise determination of TPM concentration in seafood samples.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts
T1  - Tropomyosin quantification in seafood samples-right choice of standard makes a difference
SP  - 132
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6024
ER  - 

@conference{
author = "Krstić-Ristivojević, Maja and Jovanović, Vesna and Radomirović, Mirjana Ž. and Trifunović, Olga and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "In the last 50 years, the annual per capita consumption of seafood products worldwide has more than doubled, from almost 10 kg in 1960 to over 20 kg in 2014. Seafood protein is an essential part of the diet in many countries, particularly where total protein intake is low [1]. However, as defined by the European Community, fish, and shellfish tropomyosins (TPM) are major allergens and major causes of anaphylaxis [2]. The increasing prevalence of food allergies is consistent with the increasing pollution of soil and water with plastic particles. To investigate the potential link between increasing plastic pollution and increasing food allergy prevalence, we aim to develop methods for precise and accurate monitoring of allergens and plastic in real seafood samples.
TPM was isolated from shrimp (Litopenaeus vannamei), clams (Venerupis philippinarum), and mussels (Mytilus galloprovincialis). The obtained in-house TPM proteins from three different sources were resolved using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The concentration of TPM in seashell samples from different geographical origin was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with prior optimization of adequate TPM standard curve using commercial and non-commercial in-house prepared TPM standards.
TPM standards resolved via 2D-PAGE revealed the presence of two isoforms of shrimp and mussels TPM standard, one dominant and one less abundant isoform. Two isoforms from both seafood sources, shrimp and mussels, are slightly different in molecular weight and pI value. As for the TPM standard obtained from clams, the 2D electrophoregram showed possibly eight isoforms with small differences in mass and pI values. Furthermore, the presence of three dominant isoforms can be observed that differ slightly in molecular mass, while other isoforms also differ in pI value. The ELISA results, regarding TPM standard curve optimization, showed that in both the commercial shrimp TPM and in-house shrimp TPM standards, sigmoidal concentration dependence is present in a range of 50 to 0.05 ng/ml, using serial double dilutions. On the other hand, TPM standards isolated from mussels and clams show sigmoidal concentration dependence in the range of 45 to 0.044 μg/ml with using the identical combination of capture and detection antibodies and serial double dilutions. TPM concentrations in clams and mussel samples extrapolated from standard curves of commercial shrimp TPM standard and corresponding in-house TPM standards are presented in Table 1.
Differences in TPM concentration of the same sample using different TPM standards differ from 40 to 600 times, which strongly indicates that the right choice of TPM standard is a critical step for accurate and precise determination of TPM concentration in seafood samples.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts",
title = "Tropomyosin quantification in seafood samples-right choice of standard makes a difference",
pages = "132-132",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6024"
}

Krstić-Ristivojević, M., Jovanović, V., Radomirović, M. Ž., Trifunović, O., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Tropomyosin quantification in seafood samples-right choice of standard makes a difference. in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts
Beograd : Srpsko hemijsko društvo., 132-132.
https://hdl.handle.net/21.15107/rcub_cherry_6024

Krstić-Ristivojević M, Jovanović V, Radomirović MŽ, Trifunović O, Stanić-Vučinić D, Ćirković-Veličković T. Tropomyosin quantification in seafood samples-right choice of standard makes a difference. in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts. 2023;:132-132.
https://hdl.handle.net/21.15107/rcub_cherry_6024 .

Krstić-Ristivojević, Maja, Jovanović, Vesna, Radomirović, Mirjana Ž., Trifunović, Olga, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Tropomyosin quantification in seafood samples-right choice of standard makes a difference" in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts (2023):132-132,
https://hdl.handle.net/21.15107/rcub_cherry_6024 .

TY  - THES
AU  - Jovanović, Višnja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6104
AB  - Porast svetske populacije uz iscrpljivanje prirodnih resursa na Zemlji, pokrenulo je pitanje i održivost postojenje poljoprivredne proizvodnje u obezbeđivanju dovoljne količine bezbedne hrane u budućnosti. Jestivi insekti su se nametnuli kao alternativa postojećom konvecionalnom stočarstvu i živinarstvu, zbog manjeg uticaja na životnu sredinu i bolje konverzije proteina hrane u proteine insekata u odnosu na svinje, ovcem goveda ili piliće. Mada su jestivi insekti kroz istoriju bili deo tradicionalne ishane za narode koji su vekovima živeli na teritoriji jugo-istočne Azije, Australije, Južne Amerike i Australije, za većinu stanovnika Evrope, Azije i severne Azije oni su hrana koja treba da bude prihvaćena obzirom na osećaj odvratnosti koje stanovnici ovih zemalja imaju prema insektima. Osim ovoga, pitanja kao što su uzgoj insekata na industrijskoj skali, automatizaciju celog postupka gajenja i obrade insekata, mikrobiološka i hemijska ispravnost ovih proizvoda i alergeni potencijal nove hrane još nisu dobila odgovor.
U ovom diplomskom radu, prvo je razvijen je protokol za izolovanje i prečiščavanje tropomiozina iz brašnjavog crva (Tenebrio molitore). Količina i elektroforetski profil proteina u pojedinim fazama izolovanja i prečićavanja upoređeni su vizuelno pomoću SDS PAGE. Nakon poslednjeg koraka prečišćavanja na jonoizmenjivačkoj koloni na Q-Sepharose dobijene su dve frakcije, koje su rasoljene i skoncentrovane i u njima je određena koncentracija proteina BCA metodom. U sledećem koraku izoforme tropomiozina iz dobijene dve frakcije su okarakterisane po molekulskoj masi i pI vrednosti na osnovu 2D SDS elektroforeze, a zatim su dalje analizirane masenom spektroskopijom nakon tripsinske digestije. U poslednjem koraku, imunoblotom je upoređena specifičnost vezivanja izolovanog tropomiozina iz brašnjavog crva i drugih beskičmenjaka kao što su kozice, dagnje, školjke i cvrčci prema mišijem anti-kozice tropomiozin monoklonskom antitelu.
T1  - Izolovanje i karakterizacija izoformi tropomiozina iz brašnjavog crva (Tenebrio molitor)
SP  - 1
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6104
ER  - 

@misc{
author = "Jovanović, Višnja",
year = "2023",
abstract = "Porast svetske populacije uz iscrpljivanje prirodnih resursa na Zemlji, pokrenulo je pitanje i održivost postojenje poljoprivredne proizvodnje u obezbeđivanju dovoljne količine bezbedne hrane u budućnosti. Jestivi insekti su se nametnuli kao alternativa postojećom konvecionalnom stočarstvu i živinarstvu, zbog manjeg uticaja na životnu sredinu i bolje konverzije proteina hrane u proteine insekata u odnosu na svinje, ovcem goveda ili piliće. Mada su jestivi insekti kroz istoriju bili deo tradicionalne ishane za narode koji su vekovima živeli na teritoriji jugo-istočne Azije, Australije, Južne Amerike i Australije, za većinu stanovnika Evrope, Azije i severne Azije oni su hrana koja treba da bude prihvaćena obzirom na osećaj odvratnosti koje stanovnici ovih zemalja imaju prema insektima. Osim ovoga, pitanja kao što su uzgoj insekata na industrijskoj skali, automatizaciju celog postupka gajenja i obrade insekata, mikrobiološka i hemijska ispravnost ovih proizvoda i alergeni potencijal nove hrane još nisu dobila odgovor.
U ovom diplomskom radu, prvo je razvijen je protokol za izolovanje i prečiščavanje tropomiozina iz brašnjavog crva (Tenebrio molitore). Količina i elektroforetski profil proteina u pojedinim fazama izolovanja i prečićavanja upoređeni su vizuelno pomoću SDS PAGE. Nakon poslednjeg koraka prečišćavanja na jonoizmenjivačkoj koloni na Q-Sepharose dobijene su dve frakcije, koje su rasoljene i skoncentrovane i u njima je određena koncentracija proteina BCA metodom. U sledećem koraku izoforme tropomiozina iz dobijene dve frakcije su okarakterisane po molekulskoj masi i pI vrednosti na osnovu 2D SDS elektroforeze, a zatim su dalje analizirane masenom spektroskopijom nakon tripsinske digestije. U poslednjem koraku, imunoblotom je upoređena specifičnost vezivanja izolovanog tropomiozina iz brašnjavog crva i drugih beskičmenjaka kao što su kozice, dagnje, školjke i cvrčci prema mišijem anti-kozice tropomiozin monoklonskom antitelu.",
title = "Izolovanje i karakterizacija izoformi tropomiozina iz brašnjavog crva (Tenebrio molitor)",
pages = "1-53",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6104"
}

Jovanović, V.. (2023). Izolovanje i karakterizacija izoformi tropomiozina iz brašnjavog crva (Tenebrio molitor). , 1-53.
https://hdl.handle.net/21.15107/rcub_cherry_6104

Jovanović V. Izolovanje i karakterizacija izoformi tropomiozina iz brašnjavog crva (Tenebrio molitor). 2023;:1-53.
https://hdl.handle.net/21.15107/rcub_cherry_6104 .

Jovanović, Višnja, "Izolovanje i karakterizacija izoformi tropomiozina iz brašnjavog crva (Tenebrio molitor)" (2023):1-53,
https://hdl.handle.net/21.15107/rcub_cherry_6104 .

TY  - THES
AU  - Pismestrović, Marina
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6138
AB  - In vitro metode koje simuliraju procese digestije se široko koriste za proučavanje 
gastrointestinalnog varenja hrane ili farmaceutskih proizvoda. Iako se nutricionističke studije još 
uvek smatraju “zlatnim standardom” za rešavanje pitanja vezanih za ishranu, in vitro metode imaju 
prednost zato što su brže, jeftinije, manje invazivne i nemaju etička ograničenja. Ovakve prednosti 
omogućavaju dalje skrining većeg broja uzoraka paralelno. Takođe, reproducibilnost, 
kontrolisanost uslova i lako uzorkovanje čine in vitro modele veoma pogodnim za istraživanja i 
postavljanje hipoteza. 
U ovom radu ispitana je kinetika ekstrakcije ukupnih proteina sirovih i kuvanih zapadno-pacifičkih 
kozica (lat. Solenocera melantho) u različitim fiziološki relevantnim tečnostima. Količina i 
elektroforetski profil ekstrahovanih ukupnih proteina upoređeni su vizuelno pomoću SDS PAGE 
i histogramski nakon Bredfordove metode za određivanje ukupne koncentracije proteina u datim 
alikvotima. Prateći in vitro standardizovani statički protokol tzv. INFOGEST urađene su bile tri 
različite digestije sirovih i kuvanih kozica. Imunogenost peptidnih fragmenata dobijenih u 
različitim fazama digestije ispitana je Western blotom korišćenjem poliklonskih anti-TPM antitela
i seruma osoba alergičnih na morske plodove i grinje. Talozi zaostali nakon završene gastrične i 
intestinalne faze zasebno su ispitani u slučaju da neki od imunogenih peptidnih fragmenata pada 
u talog nakon/tokom centrifugiranja pre spremanja uzorka za elektroforezu. Finalno, urađena je 
ELISA zarad kvantifikacije TPM-a u ekstraktima sirovih kozica u simuliranim fiziološkim 
tečnostima, kao i alikvotima uzetih tokom in vitro digestije nakon završene gastrične i intestinalne
faze.
T1  - Analiza imunoreaktivnosti peptida i proteina  kozica dobijenih u simuliranim uslovima  gastrointestinalne digestije korišćenjem  INFOGEST protokola
SP  - 2
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6138
ER  - 

@mastersthesis{
author = "Pismestrović, Marina",
year = "2023",
abstract = "In vitro metode koje simuliraju procese digestije se široko koriste za proučavanje 
gastrointestinalnog varenja hrane ili farmaceutskih proizvoda. Iako se nutricionističke studije još 
uvek smatraju “zlatnim standardom” za rešavanje pitanja vezanih za ishranu, in vitro metode imaju 
prednost zato što su brže, jeftinije, manje invazivne i nemaju etička ograničenja. Ovakve prednosti 
omogućavaju dalje skrining većeg broja uzoraka paralelno. Takođe, reproducibilnost, 
kontrolisanost uslova i lako uzorkovanje čine in vitro modele veoma pogodnim za istraživanja i 
postavljanje hipoteza. 
U ovom radu ispitana je kinetika ekstrakcije ukupnih proteina sirovih i kuvanih zapadno-pacifičkih 
kozica (lat. Solenocera melantho) u različitim fiziološki relevantnim tečnostima. Količina i 
elektroforetski profil ekstrahovanih ukupnih proteina upoređeni su vizuelno pomoću SDS PAGE 
i histogramski nakon Bredfordove metode za određivanje ukupne koncentracije proteina u datim 
alikvotima. Prateći in vitro standardizovani statički protokol tzv. INFOGEST urađene su bile tri 
različite digestije sirovih i kuvanih kozica. Imunogenost peptidnih fragmenata dobijenih u 
različitim fazama digestije ispitana je Western blotom korišćenjem poliklonskih anti-TPM antitela
i seruma osoba alergičnih na morske plodove i grinje. Talozi zaostali nakon završene gastrične i 
intestinalne faze zasebno su ispitani u slučaju da neki od imunogenih peptidnih fragmenata pada 
u talog nakon/tokom centrifugiranja pre spremanja uzorka za elektroforezu. Finalno, urađena je 
ELISA zarad kvantifikacije TPM-a u ekstraktima sirovih kozica u simuliranim fiziološkim 
tečnostima, kao i alikvotima uzetih tokom in vitro digestije nakon završene gastrične i intestinalne
faze.",
title = "Analiza imunoreaktivnosti peptida i proteina  kozica dobijenih u simuliranim uslovima  gastrointestinalne digestije korišćenjem  INFOGEST protokola",
pages = "2-76",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6138"
}

Pismestrović, M.. (2023). Analiza imunoreaktivnosti peptida i proteina  kozica dobijenih u simuliranim uslovima  gastrointestinalne digestije korišćenjem  INFOGEST protokola. , 2-76.
https://hdl.handle.net/21.15107/rcub_cherry_6138

Pismestrović M. Analiza imunoreaktivnosti peptida i proteina  kozica dobijenih u simuliranim uslovima  gastrointestinalne digestije korišćenjem  INFOGEST protokola. 2023;:2-76.
https://hdl.handle.net/21.15107/rcub_cherry_6138 .

Pismestrović, Marina, "Analiza imunoreaktivnosti peptida i proteina  kozica dobijenih u simuliranim uslovima  gastrointestinalne digestije korišćenjem  INFOGEST protokola" (2023):2-76,
https://hdl.handle.net/21.15107/rcub_cherry_6138 .

TY  - CONF
AU  - Lujić, Tamara
AU  - Gligorijević, Nikola
AU  - Jovanović, Vesna
AU  - Aćimović, Jelena
AU  - Mitić, Dragana
AU  - Vasović, Tamara
AU  - Stojadinović, Marija
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6424
AB  - Microplastics is abundant in the environment, food and beverages and get ingested by humans. Its complex interplay with proteins lead to formation of corona. Tightly bound proteins represent hard corona, while weaker binding partners are found in soft corona. Separation of hard and soft corona of allergenic proteins of shrimps, eggs and cow’s milk, tropomyosin (TPM), ovalbumin (OVA) and beta-lactoglobulin (BLG) and identification of binding partners by proteomics was aim of our study.
Allergenic proteins were purified from egg white, shrimps and cow’s milk. Binding to polyethylene terephthalate microplastics (PET) (70-100 m) was probed at pH 7 for purified allergens and egg white proteins. After establishment of binding equilibrium, soft and hard corona were separated and analyzed by SDS PAGE, followed by identification of bound proteins by nanoLC-HRMS. Binding of all allergenic proteins was observed in both soft and hard corona. Soft corona contains exclusively intact, full length OVA, TPM and BLG. Hard corona is enriched for truncated OVA and oligomers of TPM. OVA fragments are partially or fully enfolded and have higher level of exposed hydrophobic patches resulting in higher affinity for PET microplastics. In comparison to OVA and TPM, hard corona of BLG is less abundant under similar conditions. BLG is compact globular protein with lower level of exposed hydrophobic patches in comparison to ovalbumin and tropomyosin. In hard corona, trace amounts of contaminating alfa-lactalbumin become enriched. In the presence of egg white protein extract OVA forms both SC and HC on microplastics, being the dominant protein of hard corona (with ovotransferrin). Lysozyme and ovomucin are present only in hard corona. Both proteins are known for their strong bioactivity and represent a small fraction of total egg white proteins.
Our results show that allergenic proteins form hard corona on PET microplastics. Among egg white proteins, minor proteins such as lysozyme and ovomucin become enriched. Denaturing effect of strong binding to microplastics may change functional characteristics of allergens and bioactive proteins of foods and should be further investigated in functional assays.
Acknowledgment: This study was supported by IMPTOX European Union's Horizon 2020 research and innovation program (grant number 965173).
PB  - Italian Proteomics Association
C3  - ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
T1  - Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6424
ER  - 

@conference{
author = "Lujić, Tamara and Gligorijević, Nikola and Jovanović, Vesna and Aćimović, Jelena and Mitić, Dragana and Vasović, Tamara and Stojadinović, Marija and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Microplastics is abundant in the environment, food and beverages and get ingested by humans. Its complex interplay with proteins lead to formation of corona. Tightly bound proteins represent hard corona, while weaker binding partners are found in soft corona. Separation of hard and soft corona of allergenic proteins of shrimps, eggs and cow’s milk, tropomyosin (TPM), ovalbumin (OVA) and beta-lactoglobulin (BLG) and identification of binding partners by proteomics was aim of our study.
Allergenic proteins were purified from egg white, shrimps and cow’s milk. Binding to polyethylene terephthalate microplastics (PET) (70-100 m) was probed at pH 7 for purified allergens and egg white proteins. After establishment of binding equilibrium, soft and hard corona were separated and analyzed by SDS PAGE, followed by identification of bound proteins by nanoLC-HRMS. Binding of all allergenic proteins was observed in both soft and hard corona. Soft corona contains exclusively intact, full length OVA, TPM and BLG. Hard corona is enriched for truncated OVA and oligomers of TPM. OVA fragments are partially or fully enfolded and have higher level of exposed hydrophobic patches resulting in higher affinity for PET microplastics. In comparison to OVA and TPM, hard corona of BLG is less abundant under similar conditions. BLG is compact globular protein with lower level of exposed hydrophobic patches in comparison to ovalbumin and tropomyosin. In hard corona, trace amounts of contaminating alfa-lactalbumin become enriched. In the presence of egg white protein extract OVA forms both SC and HC on microplastics, being the dominant protein of hard corona (with ovotransferrin). Lysozyme and ovomucin are present only in hard corona. Both proteins are known for their strong bioactivity and represent a small fraction of total egg white proteins.
Our results show that allergenic proteins form hard corona on PET microplastics. Among egg white proteins, minor proteins such as lysozyme and ovomucin become enriched. Denaturing effect of strong binding to microplastics may change functional characteristics of allergens and bioactive proteins of foods and should be further investigated in functional assays.
Acknowledgment: This study was supported by IMPTOX European Union's Horizon 2020 research and innovation program (grant number 965173).",
publisher = "Italian Proteomics Association",
journal = "ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy",
title = "Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6424"
}

Lujić, T., Gligorijević, N., Jovanović, V., Aćimović, J., Mitić, D., Vasović, T., Stojadinović, M., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
Italian Proteomics Association., 11-11.
https://hdl.handle.net/21.15107/rcub_cherry_6424

Lujić T, Gligorijević N, Jovanović V, Aćimović J, Mitić D, Vasović T, Stojadinović M, Stanić-Vučinić D, Ćirković-Veličković T. Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy. 2023;:11-11.
https://hdl.handle.net/21.15107/rcub_cherry_6424 .

Lujić, Tamara, Gligorijević, Nikola, Jovanović, Vesna, Aćimović, Jelena, Mitić, Dragana, Vasović, Tamara, Stojadinović, Marija, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics" in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy (2023):11-11,
https://hdl.handle.net/21.15107/rcub_cherry_6424 .

TY  - DATA
AU  - Radomirović, Mirjana
AU  - Gligorijević, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6466
AB  - Experimental data for the results shown in the manuscript Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR are given in the attachment.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Research data for: Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences MDPI., 24(20), 15410. https://doi.org/doi.org/10.3390/ ijms242015410
VL  - 24
IS  - 20
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6466
ER  - 

@misc{
author = "Radomirović, Mirjana and Gligorijević, Nikola and Stanić-Vučinić, Dragana and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Experimental data for the results shown in the manuscript Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR are given in the attachment.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Research data for: Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences MDPI., 24(20), 15410. https://doi.org/doi.org/10.3390/ ijms242015410",
volume = "24",
number = "20",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6466"
}

Radomirović, M., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Research data for: Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences MDPI., 24(20), 15410. https://doi.org/doi.org/10.3390/ ijms242015410. in International Journal of Molecular Sciences
MDPI., 24(20).
https://hdl.handle.net/21.15107/rcub_cherry_6466

Radomirović M, Gligorijević N, Stanić-Vučinić D, Rajković A, Ćirković-Veličković T. Research data for: Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences MDPI., 24(20), 15410. https://doi.org/doi.org/10.3390/ ijms242015410. in International Journal of Molecular Sciences. 2023;24(20).
https://hdl.handle.net/21.15107/rcub_cherry_6466 .

Radomirović, Mirjana, Gligorijević, Nikola, Stanić-Vučinić, Dragana, Rajković, Andreja, Ćirković-Veličković, Tanja, "Research data for: Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences MDPI., 24(20), 15410. https://doi.org/doi.org/10.3390/ ijms242015410" in International Journal of Molecular Sciences, 24, no. 20 (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6466 .

TY  - JOUR
AU  - Radomirović, Mirjana Ž.
AU  - Gligorijević, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6019
AB  - Tropomyosin is the major and predominant allergen among shellfish. This study developed
an ultrasensitive immuno-PCR method for the quantification of crustacean tropomyosin in foods.
The method couples sandwich ELISA with the real-time PCR (rtPCR) amplification of marker DNAs. Monoclonal anti-TPM antibody was the capture antibody, polyclonal rabbit anti-shrimp tropomyosin antibody was the detection antibody, while natural shrimp tropomyosin served as the standard. A double-stranded amino-DNA was covalently conjugated to a secondary anti-rabbit antibody and subsequently amplified and quantified via rtPCR. The quantification sensitivity of immuno-PCR was 20-fold higher than analogous ELISA, with LOQ 19.8 pg/mL. The developed immuno-PCR method is highly specific for the detection of crustacean tropomyosin and is highly precise in a broad concentration range. Tropomyosin recovery in the spiked vegetable soup was 87.7–115.6%. Crustacean tropomyosin was also quantified in commercial food products. The reported immuno-PCR assay is the most sensitive method for the quantification of crustacean tropomyosin and is the first immuno-PCR-based assay for the quantification of food allergen and food protein in general. The described method could be easily adapted for the specific and ultrasensitive immuno-PCR-based detection of traces of any food allergen that is currently being quantified with ELISA, which is of critical importance for people with food allergies.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR
VL  - 24
IS  - 20
SP  - 15410
DO  - doi.org/10.3390/ ijms242015410
ER  - 

@article{
author = "Radomirović, Mirjana Ž. and Gligorijević, Nikola and Stanić-Vučinić, Dragana and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Tropomyosin is the major and predominant allergen among shellfish. This study developed
an ultrasensitive immuno-PCR method for the quantification of crustacean tropomyosin in foods.
The method couples sandwich ELISA with the real-time PCR (rtPCR) amplification of marker DNAs. Monoclonal anti-TPM antibody was the capture antibody, polyclonal rabbit anti-shrimp tropomyosin antibody was the detection antibody, while natural shrimp tropomyosin served as the standard. A double-stranded amino-DNA was covalently conjugated to a secondary anti-rabbit antibody and subsequently amplified and quantified via rtPCR. The quantification sensitivity of immuno-PCR was 20-fold higher than analogous ELISA, with LOQ 19.8 pg/mL. The developed immuno-PCR method is highly specific for the detection of crustacean tropomyosin and is highly precise in a broad concentration range. Tropomyosin recovery in the spiked vegetable soup was 87.7–115.6%. Crustacean tropomyosin was also quantified in commercial food products. The reported immuno-PCR assay is the most sensitive method for the quantification of crustacean tropomyosin and is the first immuno-PCR-based assay for the quantification of food allergen and food protein in general. The described method could be easily adapted for the specific and ultrasensitive immuno-PCR-based detection of traces of any food allergen that is currently being quantified with ELISA, which is of critical importance for people with food allergies.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR",
volume = "24",
number = "20",
pages = "15410",
doi = "doi.org/10.3390/ ijms242015410"
}

Radomirović, M. Ž., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković-Veličković, T.. (2023). Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences
MDPI., 24(20), 15410.
https://doi.org/doi.org/10.3390/ ijms242015410

Radomirović MŽ, Gligorijević N, Stanić-Vučinić D, Rajković A, Ćirković-Veličković T. Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR. in International Journal of Molecular Sciences. 2023;24(20):15410.
doi:doi.org/10.3390/ ijms242015410 .

Radomirović, Mirjana Ž., Gligorijević, Nikola, Stanić-Vučinić, Dragana, Rajković, Andreja, Ćirković-Veličković, Tanja, "Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR" in International Journal of Molecular Sciences, 24, no. 20 (2023):15410,
https://doi.org/doi.org/10.3390/ ijms242015410 . .

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Vasović, Tamara
AU  - Smiljanić, Katarina
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5951
AB  - rom over 10 kg in 1960 to over 20 kg in 2014, the yearly per capita consumption of
marine products has increased significantly during the past 50 years. In many nations,
especially those with poor overall protein intake, seafood protein is a crucial component of
the diet1. However, as defined by the European Community shellfish protein tropomyosin
(TPM) is one of the major allergens and major causes of anaphylaxis 2. Although TPM
originating from vertebrates is not considered as an allergen there is evidence that several
fish tropomyosin can be allergenic3. TPM protein is organized of two parallel alpha-helical
molecules which are wound around each other forming a coiled -coil structure2. Although
the degree of similarity between TPM molecules is high, their allergenic potency is
different. Scientists putting a lot of effort into iden tifying and sequencing tropomyosin
isoforms since this information probably explains the intriguing nature of the TPM
molecule. This is a very challenging task given that the differences in mass and pI values
between TPM isoforms are discrete.
TPM was isolated from mussels (Mytilus galloprovincialis), and clams (Venerupis
philippinarum) according to the protocol developed within/and for purposes of the
IMPTOX research project. The obtained “in-house” TPM proteins were resolved using
two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Isoelectric focusing was
performed on rehydrated Immobiline strips IPG 3-5.6NL 13 cm in Ettan IPGPhor 3 device.
The second dimension was carried out on 12% PAA gels. Upon protein bands excision to
prevent TPM interchain disulfide cross-linking reduction and alkylation of cysteine was
performed. The samples were digested with proteomics-grade trypsin in a 1:50 enzyme-to-
substrate ratio overnight at 37 °C. The obtained peptides were chromatographically
separated using the EASY-nLC II system and analyzed using Orbitrap Exploris 240 mass
spectrometer.
2D-PAGE resolved two isoforms of mussel TPM and up to eight isoforms of clam TPM.
The determined pI value of the dominant isoform of mussel TPM was 4.7 while the
discrete band arising from the second TPM isoform was slightly shifted toward acidic pI
and smaller molecular mass. The determined pI value of the three most dominant clam
TPM isoforms was 4.8, the fourth isoform was slightly shifted toward a more basic pI
value and lower protein molecular weight. The rest of the isoforms were slightly shifted
toward more acidic pI and at a similar molecular weight as the three dominant isoforms.
PB  - Kragujevac : Univerzitet, Prirodno-matematički fakultet
C3  - Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac
T1  - Identification of isoforms of shelfish tropomyosin
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5951
ER  - 

@conference{
author = "Krstić-Ristivojević, Maja and Vasović, Tamara and Smiljanić, Katarina and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "rom over 10 kg in 1960 to over 20 kg in 2014, the yearly per capita consumption of
marine products has increased significantly during the past 50 years. In many nations,
especially those with poor overall protein intake, seafood protein is a crucial component of
the diet1. However, as defined by the European Community shellfish protein tropomyosin
(TPM) is one of the major allergens and major causes of anaphylaxis 2. Although TPM
originating from vertebrates is not considered as an allergen there is evidence that several
fish tropomyosin can be allergenic3. TPM protein is organized of two parallel alpha-helical
molecules which are wound around each other forming a coiled -coil structure2. Although
the degree of similarity between TPM molecules is high, their allergenic potency is
different. Scientists putting a lot of effort into iden tifying and sequencing tropomyosin
isoforms since this information probably explains the intriguing nature of the TPM
molecule. This is a very challenging task given that the differences in mass and pI values
between TPM isoforms are discrete.
TPM was isolated from mussels (Mytilus galloprovincialis), and clams (Venerupis
philippinarum) according to the protocol developed within/and for purposes of the
IMPTOX research project. The obtained “in-house” TPM proteins were resolved using
two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Isoelectric focusing was
performed on rehydrated Immobiline strips IPG 3-5.6NL 13 cm in Ettan IPGPhor 3 device.
The second dimension was carried out on 12% PAA gels. Upon protein bands excision to
prevent TPM interchain disulfide cross-linking reduction and alkylation of cysteine was
performed. The samples were digested with proteomics-grade trypsin in a 1:50 enzyme-to-
substrate ratio overnight at 37 °C. The obtained peptides were chromatographically
separated using the EASY-nLC II system and analyzed using Orbitrap Exploris 240 mass
spectrometer.
2D-PAGE resolved two isoforms of mussel TPM and up to eight isoforms of clam TPM.
The determined pI value of the dominant isoform of mussel TPM was 4.7 while the
discrete band arising from the second TPM isoform was slightly shifted toward acidic pI
and smaller molecular mass. The determined pI value of the three most dominant clam
TPM isoforms was 4.8, the fourth isoform was slightly shifted toward a more basic pI
value and lower protein molecular weight. The rest of the isoforms were slightly shifted
toward more acidic pI and at a similar molecular weight as the three dominant isoforms.",
publisher = "Kragujevac : Univerzitet, Prirodno-matematički fakultet",
journal = "Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac",
title = "Identification of isoforms of shelfish tropomyosin",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5951"
}

Krstić-Ristivojević, M., Vasović, T., Smiljanić, K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Identification of isoforms of shelfish tropomyosin. in Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac
Kragujevac : Univerzitet, Prirodno-matematički fakultet..
https://hdl.handle.net/21.15107/rcub_cherry_5951

Krstić-Ristivojević M, Vasović T, Smiljanić K, Stanić-Vučinić D, Ćirković-Veličković T. Identification of isoforms of shelfish tropomyosin. in Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_5951 .

Krstić-Ristivojević, Maja, Vasović, Tamara, Smiljanić, Katarina, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Identification of isoforms of shelfish tropomyosin" in Zbornik apstrakata, VI Simpozijum Srpskog udruženja za proteomiku (SePA) “Razvoj i primena novih metoda proteomike”, 2. jun 2023. godine, Kragujevac (2023),
https://hdl.handle.net/21.15107/rcub_cherry_5951 .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Mutić, Tamara
AU  - Lujić, Tamara
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5909
AB  - Microplastic represents one of the major types of pollutants in modern era. Over several years of research in the field of microplastic,
there are still many unknown gaps, including the effects and mechanisms of action of these particles on human
health. Studies in this field conducted experiments on cells and human tissues or animals like rats and mice. While these
studies suggest the toxic effects of microplastic, it is not clear if concentrations used for exposure are relevant for humans.
Also, most of the studies used spherical polystyrene, which does not reflect well the diversity of microplastic particles found
in nature. Another gap is lack of studies describing direct interactions of microplastics and proteins. While it is generally
known that proteins form corona around microplastic particles, affinity studies and consequences on protein structure are
usually missing.
The aim of this work was to analyze interaction of a major egg white protein and allergen, ovalbumin to several to microplastic
particles, including polystyrene (PS) of 120 and 500 μm in size and polyethylene terephthalate (PET) of 120 μm in
size. Binding affinity was determined at both acidic, pH 3 and neutral, pH 7 conditions, at the room temperature, by measuring
bulk ovalbumin concentration in supernatants at the equilibrium time. Several binding models, including Langmuir,
Freundlich, Redlich–Peterson and Guggenheim-Anderson-de Boer (GAB), were used to determine binding parameters.
The formation of soft and hard corona was analyzed according to the published protocol [1]. Structural analysis was performed
using near and far-UV CD spectrometry.
Obtained results showed that ovalbumin binds to both PS and PET. All binding models indicated that ovalbumin binds
with higher affinity to tested microplastics on pH 3, compared to pH 7, with the highest affinity being calculated for PS 120
μm. Further analysis showed that ovalbumin forms both soft and hard corona onto the surface of all three microplastics.
Structural alterations of ovalbumin as a consequence of its interaction with microplastic was shown to be both pH and
microplastic type dependent. Also, more pronounced effect on its tertiary structure was observed, compared to secondary.
At pH3, tertiary structure of bulk ovalbumin becomes destabilized, especially in the presence of PET 120 μm and PS 500
μm, while at pH 7, structural stabilization is observed, especially in the presence of PS 120 μm.
Considering that the microplastic was discovered in eggs [2], obtained results suggest that direct interactions of native
ovalbumin with microplastic particles could have influence on its structure and thus affect its techno-functional properties.
Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation programme
under grant agreement No 96517.
References:
[1] D. Magrì, P. Sánchez-Moreno, G. Caputo, F. Gatto, M. Veronesi, G. Bardi, T. Catelani, D. Guarnieri, A. Athanassiou, P.P. Pompa, D.
Fragouli, ACS Nano, 12 (2018) 7690-7700.
[2] Q. Liu, Z. Chen, Y. Chen, F. Yang, W. Yao, Y. Xie, Food Chemistry, 397 (2022) 133771
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
T1  - Binding and corona formation of ovalbumin to polystyrene and polyethylene terephthalate microplastics under neutral and acidic conditions
SP  - 137
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5909
ER  - 

@conference{
author = "Gligorijević, Nikola and Stanić-Vučinić, Dragana and Mutić, Tamara and Lujić, Tamara and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Microplastic represents one of the major types of pollutants in modern era. Over several years of research in the field of microplastic,
there are still many unknown gaps, including the effects and mechanisms of action of these particles on human
health. Studies in this field conducted experiments on cells and human tissues or animals like rats and mice. While these
studies suggest the toxic effects of microplastic, it is not clear if concentrations used for exposure are relevant for humans.
Also, most of the studies used spherical polystyrene, which does not reflect well the diversity of microplastic particles found
in nature. Another gap is lack of studies describing direct interactions of microplastics and proteins. While it is generally
known that proteins form corona around microplastic particles, affinity studies and consequences on protein structure are
usually missing.
The aim of this work was to analyze interaction of a major egg white protein and allergen, ovalbumin to several to microplastic
particles, including polystyrene (PS) of 120 and 500 μm in size and polyethylene terephthalate (PET) of 120 μm in
size. Binding affinity was determined at both acidic, pH 3 and neutral, pH 7 conditions, at the room temperature, by measuring
bulk ovalbumin concentration in supernatants at the equilibrium time. Several binding models, including Langmuir,
Freundlich, Redlich–Peterson and Guggenheim-Anderson-de Boer (GAB), were used to determine binding parameters.
The formation of soft and hard corona was analyzed according to the published protocol [1]. Structural analysis was performed
using near and far-UV CD spectrometry.
Obtained results showed that ovalbumin binds to both PS and PET. All binding models indicated that ovalbumin binds
with higher affinity to tested microplastics on pH 3, compared to pH 7, with the highest affinity being calculated for PS 120
μm. Further analysis showed that ovalbumin forms both soft and hard corona onto the surface of all three microplastics.
Structural alterations of ovalbumin as a consequence of its interaction with microplastic was shown to be both pH and
microplastic type dependent. Also, more pronounced effect on its tertiary structure was observed, compared to secondary.
At pH3, tertiary structure of bulk ovalbumin becomes destabilized, especially in the presence of PET 120 μm and PS 500
μm, while at pH 7, structural stabilization is observed, especially in the presence of PS 120 μm.
Considering that the microplastic was discovered in eggs [2], obtained results suggest that direct interactions of native
ovalbumin with microplastic particles could have influence on its structure and thus affect its techno-functional properties.
Acknowledgments: This project has received funding from the European Union’s Horizon 2020 research and innovation programme
under grant agreement No 96517.
References:
[1] D. Magrì, P. Sánchez-Moreno, G. Caputo, F. Gatto, M. Veronesi, G. Bardi, T. Catelani, D. Guarnieri, A. Athanassiou, P.P. Pompa, D.
Fragouli, ACS Nano, 12 (2018) 7690-7700.
[2] Q. Liu, Z. Chen, Y. Chen, F. Yang, W. Yao, Y. Xie, Food Chemistry, 397 (2022) 133771",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023",
title = "Binding and corona formation of ovalbumin to polystyrene and polyethylene terephthalate microplastics under neutral and acidic conditions",
pages = "137-137",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5909"
}

Gligorijević, N., Stanić-Vučinić, D., Mutić, T., Lujić, T.,& Ćirković-Veličković, T.. (2023). Binding and corona formation of ovalbumin to polystyrene and polyethylene terephthalate microplastics under neutral and acidic conditions. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
Belgrade : Serbian Chemical Society., 137-137.
https://hdl.handle.net/21.15107/rcub_cherry_5909

Gligorijević N, Stanić-Vučinić D, Mutić T, Lujić T, Ćirković-Veličković T. Binding and corona formation of ovalbumin to polystyrene and polyethylene terephthalate microplastics under neutral and acidic conditions. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023. 2023;:137-137.
https://hdl.handle.net/21.15107/rcub_cherry_5909 .

Gligorijević, Nikola, Stanić-Vučinić, Dragana, Mutić, Tamara, Lujić, Tamara, Ćirković-Veličković, Tanja, "Binding and corona formation of ovalbumin to polystyrene and polyethylene terephthalate microplastics under neutral and acidic conditions" in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023 (2023):137-137,
https://hdl.handle.net/21.15107/rcub_cherry_5909 .