TY  - JOUR
AU  - Ristivojević, Petar
AU  - Stević, Tatjana
AU  - Starović, M.
AU  - Pavlović, Slađan Z.
AU  - Özcan, M.M.
AU  - Berić, T.
AU  - Dimkić, Ivica
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4005
AB  - Aims: A multidisciplinary approach was used to compare phenolic composition, radical scavenging and antimicrobial activity of propolis samples from different geographical localities, and plant resin against various microorganisms. Methods and Results: Using UHPLC-qqqMS quantitative analysis, 28 phenolic compounds were determined. Caffeic and p-coumaric acids were identified as main phenolic acids in poplar propolis samples, except samples from Russia (P6) and China (P7). Radical scavenging activity (applying DPPH spectrophotometric assay) showed the highest activity of Serbian (40·51%) and Chinese (53·21%) propolis samples. Broth microdilution method was used for the oral cavity, fungal phytopathogenic and human vaginal isolates which have been identified at a molecular level. The most sensitive bacterial isolates were Lactobacillus acidophilus (MIC of 0·03–0·13 mg ml−1) and the oral streptococci isolates (MIC values of 0·19–0·13 mg ml−1). The most sensitive fungal phytopathogenic isolate was Fusarium oxysporum (MIC 0·003 mg ml−1). All samples, except propolis from Serbia (P4) and Turkey (P5), showed a strong antifungal activity against Fusarium sporotrichioides, Fusarium subglutinans and Fusarium proliferatum. Conclusion: The results of various tests indicate good radical scavenging and antimicrobial activity against important human and plant pathogens. Significance and Impact of the Study: A detailed propolis analysis is important when proposing a preparation of new biological antimicrobial products which have a positive impact on human health and reduce antibacterial resistance.
T2  - Journal of Applied Microbiology
T1  - Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species
VL  - 129
IS  - 2
SP  - 296
EP  - 310
DO  - 10.1111/jam.14633
ER  - 

@article{
author = "Ristivojević, Petar and Stević, Tatjana and Starović, M. and Pavlović, Slađan Z. and Özcan, M.M. and Berić, T. and Dimkić, Ivica",
year = "2020",
abstract = "Aims: A multidisciplinary approach was used to compare phenolic composition, radical scavenging and antimicrobial activity of propolis samples from different geographical localities, and plant resin against various microorganisms. Methods and Results: Using UHPLC-qqqMS quantitative analysis, 28 phenolic compounds were determined. Caffeic and p-coumaric acids were identified as main phenolic acids in poplar propolis samples, except samples from Russia (P6) and China (P7). Radical scavenging activity (applying DPPH spectrophotometric assay) showed the highest activity of Serbian (40·51%) and Chinese (53·21%) propolis samples. Broth microdilution method was used for the oral cavity, fungal phytopathogenic and human vaginal isolates which have been identified at a molecular level. The most sensitive bacterial isolates were Lactobacillus acidophilus (MIC of 0·03–0·13 mg ml−1) and the oral streptococci isolates (MIC values of 0·19–0·13 mg ml−1). The most sensitive fungal phytopathogenic isolate was Fusarium oxysporum (MIC 0·003 mg ml−1). All samples, except propolis from Serbia (P4) and Turkey (P5), showed a strong antifungal activity against Fusarium sporotrichioides, Fusarium subglutinans and Fusarium proliferatum. Conclusion: The results of various tests indicate good radical scavenging and antimicrobial activity against important human and plant pathogens. Significance and Impact of the Study: A detailed propolis analysis is important when proposing a preparation of new biological antimicrobial products which have a positive impact on human health and reduce antibacterial resistance.",
journal = "Journal of Applied Microbiology",
title = "Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species",
volume = "129",
number = "2",
pages = "296-310",
doi = "10.1111/jam.14633"
}

Ristivojević, P., Stević, T., Starović, M., Pavlović, S. Z., Özcan, M.M., Berić, T.,& Dimkić, I.. (2020). Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species. in Journal of Applied Microbiology, 129(2), 296-310.
https://doi.org/10.1111/jam.14633

Ristivojević P, Stević T, Starović M, Pavlović SZ, Özcan M, Berić T, Dimkić I. Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species. in Journal of Applied Microbiology. 2020;129(2):296-310.
doi:10.1111/jam.14633 .

Ristivojević, Petar, Stević, Tatjana, Starović, M., Pavlović, Slađan Z., Özcan, M.M., Berić, T., Dimkić, Ivica, "Phenolic composition and biological activities of geographically different type of propolis and black cottonwood resins against oral streptococci, vaginal microbiota and phytopathogenic Fusarium species" in Journal of Applied Microbiology, 129, no. 2 (2020):296-310,
https://doi.org/10.1111/jam.14633 . .

TY  - JOUR
AU  - Vukotić, Goran N.
AU  - Polović, Natalija
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Stanisavljević, Nemanja S.
AU  - Fira, Đorđe
AU  - Kojić, Milan O.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3298
AB  - In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.
PB  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. Lactis BGMN1-501
VL  - 10
IS  - APR
DO  - 10.3389/fmicb.2019.00874
ER  - 

@article{
author = "Vukotić, Goran N. and Polović, Natalija and Mirković, Nemanja and Jovčić, Branko and Stanisavljević, Nemanja S. and Fira, Đorđe and Kojić, Milan O.",
year = "2019",
abstract = "In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.",
publisher = "Frontiers in Microbiology",
journal = "Frontiers in Microbiology",
title = "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. Lactis BGMN1-501",
volume = "10",
number = "APR",
doi = "10.3389/fmicb.2019.00874"
}

Vukotić, G. N., Polović, N., Mirković, N., Jovčić, B., Stanisavljević, N. S., Fira, Đ.,& Kojić, M. O.. (2019). Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. Lactis BGMN1-501. in Frontiers in Microbiology
Frontiers in Microbiology., 10(APR).
https://doi.org/10.3389/fmicb.2019.00874

Vukotić GN, Polović N, Mirković N, Jovčić B, Stanisavljević NS, Fira Đ, Kojić MO. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. Lactis BGMN1-501. in Frontiers in Microbiology. 2019;10(APR).
doi:10.3389/fmicb.2019.00874 .

Vukotić, Goran N., Polović, Natalija, Mirković, Nemanja, Jovčić, Branko, Stanisavljević, Nemanja S., Fira, Đorđe, Kojić, Milan O., "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. Lactis BGMN1-501" in Frontiers in Microbiology, 10, no. APR (2019),
https://doi.org/10.3389/fmicb.2019.00874 . .

TY  - DATA
AU  - Vukotić, Goran N.
AU  - Polović, Natalija
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Stanisavljević, Nemanja S.
AU  - Fira, Đorđe
AU  - Kojić, Milan O.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3299
PB  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Supplementary material for the article: Vukotic, G.; Polovic, N.; Mirkovic, N.; Jovcic, B.; Stanisavljevic, N.; Fira, D.; Kojic, M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus Lactis Subsp. Lactis BGMN1-501. Frontiers in Microbiology 2019, 10 (APR). https://doi.org/10.3389/fmicb.2019.00874
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3299
ER  - 

@misc{
author = "Vukotić, Goran N. and Polović, Natalija and Mirković, Nemanja and Jovčić, Branko and Stanisavljević, Nemanja S. and Fira, Đorđe and Kojić, Milan O.",
year = "2019",
publisher = "Frontiers in Microbiology",
journal = "Frontiers in Microbiology",
title = "Supplementary material for the article: Vukotic, G.; Polovic, N.; Mirkovic, N.; Jovcic, B.; Stanisavljevic, N.; Fira, D.; Kojic, M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus Lactis Subsp. Lactis BGMN1-501. Frontiers in Microbiology 2019, 10 (APR). https://doi.org/10.3389/fmicb.2019.00874",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3299"
}

Vukotić, G. N., Polović, N., Mirković, N., Jovčić, B., Stanisavljević, N. S., Fira, Đ.,& Kojić, M. O.. (2019). Supplementary material for the article: Vukotic, G.; Polovic, N.; Mirkovic, N.; Jovcic, B.; Stanisavljevic, N.; Fira, D.; Kojic, M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus Lactis Subsp. Lactis BGMN1-501. Frontiers in Microbiology 2019, 10 (APR). https://doi.org/10.3389/fmicb.2019.00874. in Frontiers in Microbiology
Frontiers in Microbiology..
https://hdl.handle.net/21.15107/rcub_cherry_3299

Vukotić GN, Polović N, Mirković N, Jovčić B, Stanisavljević NS, Fira Đ, Kojić MO. Supplementary material for the article: Vukotic, G.; Polovic, N.; Mirkovic, N.; Jovcic, B.; Stanisavljevic, N.; Fira, D.; Kojic, M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus Lactis Subsp. Lactis BGMN1-501. Frontiers in Microbiology 2019, 10 (APR). https://doi.org/10.3389/fmicb.2019.00874. in Frontiers in Microbiology. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3299 .

Vukotić, Goran N., Polović, Natalija, Mirković, Nemanja, Jovčić, Branko, Stanisavljević, Nemanja S., Fira, Đorđe, Kojić, Milan O., "Supplementary material for the article: Vukotic, G.; Polovic, N.; Mirkovic, N.; Jovcic, B.; Stanisavljevic, N.; Fira, D.; Kojic, M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus Lactis Subsp. Lactis BGMN1-501. Frontiers in Microbiology 2019, 10 (APR). https://doi.org/10.3389/fmicb.2019.00874" in Frontiers in Microbiology (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3299 .

TY  - JOUR
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Opsenica, Dejan M.
AU  - Gašić, Uroš M.
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3729
AB  - European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and is traditionally processed into slivovitz brandy. The brown rot disease caused by Monilinia laxa drastically reduces plum yield almost every year. Fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Čačanska Lepotica and Čačanska Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium, and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of eleven M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist P4/16_1, which significantly reduced mycelial growth of M. laxa, was identified as Pseudomonas synxantha. Further characterization of P4/16_1 revealed the production of volatile organic compounds and phenazine-1-carboxylic acid (PCA). Crude benzene extract of PCA exhibited 57–63% mycelial growth inhibition of M. laxa. LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is the first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for further investigation for potential use as a biocontrol agent against brown rot-causing fungi.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa
VL  - 10
DO  - 10.3389/fmicb.2019.02287
ER  - 

@article{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Opsenica, Dejan M. and Gašić, Uroš M. and Stanković, Slaviša and Berić, Tanja",
year = "2019",
abstract = "European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and is traditionally processed into slivovitz brandy. The brown rot disease caused by Monilinia laxa drastically reduces plum yield almost every year. Fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Čačanska Lepotica and Čačanska Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium, and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of eleven M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist P4/16_1, which significantly reduced mycelial growth of M. laxa, was identified as Pseudomonas synxantha. Further characterization of P4/16_1 revealed the production of volatile organic compounds and phenazine-1-carboxylic acid (PCA). Crude benzene extract of PCA exhibited 57–63% mycelial growth inhibition of M. laxa. LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is the first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for further investigation for potential use as a biocontrol agent against brown rot-causing fungi.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa",
volume = "10",
doi = "10.3389/fmicb.2019.02287"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Opsenica, D. M., Gašić, U. M., Stanković, S.,& Berić, T.. (2019). Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa. in Frontiers in Microbiology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fmicb.2019.02287

Janakiev T, Dimkić I, Unković N, Ljaljević-Grbić M, Opsenica DM, Gašić UM, Stanković S, Berić T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.02287 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Opsenica, Dejan M., Gašić, Uroš M., Stanković, Slaviša, Berić, Tanja, "Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas synxantha Against Monilinia laxa" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.02287 . .

TY  - DATA
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Opsenica, Dejan M.
AU  - Gašić, Uroš M.
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3730
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3730
ER  - 

@misc{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Opsenica, Dejan M. and Gašić, Uroš M. and Stanković, Slaviša and Berić, Tanja",
year = "2019",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3730"
}

Janakiev, T., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Opsenica, D. M., Gašić, U. M., Stanković, S.,& Berić, T.. (2019). Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287. in Frontiers in Microbiology
Frontiers Media S.A.., 10.
https://hdl.handle.net/21.15107/rcub_cherry_3730

Janakiev T, Dimkić I, Unković N, Ljaljević-Grbić M, Opsenica DM, Gašić UM, Stanković S, Berić T. Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287. in Frontiers in Microbiology. 2019;10.
https://hdl.handle.net/21.15107/rcub_cherry_3730 .

Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Opsenica, Dejan M., Gašić, Uroš M., Stanković, Slaviša, Berić, Tanja, "Supplementary data for the article: Janakiev, T.; Dimkić, I.; Unković, N.; Ljaljević Grbić, M.; Opsenica, D.; Gašić, U.; Stanković, S.; Berić, T. Phyllosphere Fungal Communities of Plum and Antifungal Activity of Indigenous Phenazine-Producing Pseudomonas Synxantha Against Monilinia Laxa. Frontiers in Microbiology 2019, 10. https://doi.org/10.3389/fmicb.2019.02287" in Frontiers in Microbiology, 10 (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3730 .

TY  - JOUR
AU  - Ilic, Bojan
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Vukojević, Jelena
AU  - Vujisić, Ljubodrag V.
AU  - Tešević, Vele
AU  - Stanković, Slaviša
AU  - Makarov, Slobodan E.
AU  - Lučić, Luka
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2198
AB  - In the light of growing interest in discovering new sources of natural antimicrobial agents, we extracted and tested the efficiency of julid defensive secretions in comparison with commercially used antibiotic and antimycotics. This work involved chemical characterization of the defensive secretions of Cylindroiulus boleti (C. L. Koch, 1847), Megaphyllum bosniense (Verhoeff, 1897) and M.unilineatum (C. L. Koch, 1838), as well as in vitro evaluation of their antimicrobial activity against 11 bacteria, one yeast and eight filamentous fungi. Compounds of the analysed defensive secretions included p-benzoquinones, alkyl esters of fatty acids and ketones. Ketones were recorded for the first time in the order Julida, and they were detected in secretions of both Megaphyllum species. All three analysed defensive secretions showed antibacterial and antifungal potential against all of the tested pathogens. Staphylococcus aureus proved to be the most sensitive bacterial strain to all analysed secretions. Also, defensive secretion of M.unilineatum showed significant antibacterial potential against Bacillus subtilis. The most resistant bacterial strains in this study were Escherichia coli, Erwinia persicina and Pseudomonas syringae pv. tomato. Analysed defensive secretions achieved the strongest antifungal activity against Aspergillus parasiticus (secretions of all three millipede species), Penicillium griseofulvum (secretions of C.boleti and M.bosniense) and Cladosporium oxysporum (secretion of M.unilineatum). The most resistant mycromycetes were A.niger (to all tested defensive secretions), A.flavus (to secretion of M.unilineatum) and P.lanosum (to secretions of C.boleti and M.bosniense). Our results showed a generally lower level of activity compared to antibiotic and a significantly higher level compared to antimycotics. The results of this study elucidate and open opportunities for further research in the field of millipede chemical ecology.
PB  - Wiley, Hoboken
T2  - Journal of Applied Entomology
T1  - Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents
VL  - 142
IS  - 8
SP  - 775
EP  - 791
DO  - 10.1111/jen.12526
ER  - 

@article{
author = "Ilic, Bojan and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Vukojević, Jelena and Vujisić, Ljubodrag V. and Tešević, Vele and Stanković, Slaviša and Makarov, Slobodan E. and Lučić, Luka",
year = "2018",
abstract = "In the light of growing interest in discovering new sources of natural antimicrobial agents, we extracted and tested the efficiency of julid defensive secretions in comparison with commercially used antibiotic and antimycotics. This work involved chemical characterization of the defensive secretions of Cylindroiulus boleti (C. L. Koch, 1847), Megaphyllum bosniense (Verhoeff, 1897) and M.unilineatum (C. L. Koch, 1838), as well as in vitro evaluation of their antimicrobial activity against 11 bacteria, one yeast and eight filamentous fungi. Compounds of the analysed defensive secretions included p-benzoquinones, alkyl esters of fatty acids and ketones. Ketones were recorded for the first time in the order Julida, and they were detected in secretions of both Megaphyllum species. All three analysed defensive secretions showed antibacterial and antifungal potential against all of the tested pathogens. Staphylococcus aureus proved to be the most sensitive bacterial strain to all analysed secretions. Also, defensive secretion of M.unilineatum showed significant antibacterial potential against Bacillus subtilis. The most resistant bacterial strains in this study were Escherichia coli, Erwinia persicina and Pseudomonas syringae pv. tomato. Analysed defensive secretions achieved the strongest antifungal activity against Aspergillus parasiticus (secretions of all three millipede species), Penicillium griseofulvum (secretions of C.boleti and M.bosniense) and Cladosporium oxysporum (secretion of M.unilineatum). The most resistant mycromycetes were A.niger (to all tested defensive secretions), A.flavus (to secretion of M.unilineatum) and P.lanosum (to secretions of C.boleti and M.bosniense). Our results showed a generally lower level of activity compared to antibiotic and a significantly higher level compared to antimycotics. The results of this study elucidate and open opportunities for further research in the field of millipede chemical ecology.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Entomology",
title = "Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents",
volume = "142",
number = "8",
pages = "775-791",
doi = "10.1111/jen.12526"
}

Ilic, B., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Vukojević, J., Vujisić, L. V., Tešević, V., Stanković, S., Makarov, S. E.,& Lučić, L.. (2018). Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents. in Journal of Applied Entomology
Wiley, Hoboken., 142(8), 775-791.
https://doi.org/10.1111/jen.12526

Ilic B, Dimkić I, Unković N, Ljaljević-Grbić M, Vukojević J, Vujisić LV, Tešević V, Stanković S, Makarov SE, Lučić L. Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents. in Journal of Applied Entomology. 2018;142(8):775-791.
doi:10.1111/jen.12526 .

Ilic, Bojan, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Vukojević, Jelena, Vujisić, Ljubodrag V., Tešević, Vele, Stanković, Slaviša, Makarov, Slobodan E., Lučić, Luka, "Millipedes vs. pathogens: Defensive secretions of some julids (Diplopoda: Julida) as potential antimicrobial agents" in Journal of Applied Entomology, 142, no. 8 (2018):775-791,
https://doi.org/10.1111/jen.12526 . .

TY  - DATA
AU  - Ilic, Bojan
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević-Grbić, Milica
AU  - Vukojević, Jelena
AU  - Vujisić, Ljubodrag V.
AU  - Tešević, Vele
AU  - Stanković, Slaviša
AU  - Makarov, Slobodan E.
AU  - Lučić, Luka
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3238
PB  - Wiley, Hoboken
T2  - Journal of Applied Entomology
T1  - Supplementary material for the article: Ilić, B.; Dimkić, I.; Unković, N.; Grbić, M. L.; Vukojević, J.; Vujisić, L.; Tešević, V.; Stanković, S.; Makarov, S.; Lučić, L. Millipedes vs. Pathogens: Defensive Secretions of Some Julids (Diplopoda: Julida) as Potential Antimicrobial Agents. Journal of Applied Entomology 2018, 142 (8), 775–791. https://doi.org/10.1111/jen.12526
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3238
ER  - 

@misc{
author = "Ilic, Bojan and Dimkić, Ivica and Unković, Nikola and Ljaljević-Grbić, Milica and Vukojević, Jelena and Vujisić, Ljubodrag V. and Tešević, Vele and Stanković, Slaviša and Makarov, Slobodan E. and Lučić, Luka",
year = "2018",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Entomology",
title = "Supplementary material for the article: Ilić, B.; Dimkić, I.; Unković, N.; Grbić, M. L.; Vukojević, J.; Vujisić, L.; Tešević, V.; Stanković, S.; Makarov, S.; Lučić, L. Millipedes vs. Pathogens: Defensive Secretions of Some Julids (Diplopoda: Julida) as Potential Antimicrobial Agents. Journal of Applied Entomology 2018, 142 (8), 775–791. https://doi.org/10.1111/jen.12526",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3238"
}

Ilic, B., Dimkić, I., Unković, N., Ljaljević-Grbić, M., Vukojević, J., Vujisić, L. V., Tešević, V., Stanković, S., Makarov, S. E.,& Lučić, L.. (2018). Supplementary material for the article: Ilić, B.; Dimkić, I.; Unković, N.; Grbić, M. L.; Vukojević, J.; Vujisić, L.; Tešević, V.; Stanković, S.; Makarov, S.; Lučić, L. Millipedes vs. Pathogens: Defensive Secretions of Some Julids (Diplopoda: Julida) as Potential Antimicrobial Agents. Journal of Applied Entomology 2018, 142 (8), 775–791. https://doi.org/10.1111/jen.12526. in Journal of Applied Entomology
Wiley, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3238

Ilic B, Dimkić I, Unković N, Ljaljević-Grbić M, Vukojević J, Vujisić LV, Tešević V, Stanković S, Makarov SE, Lučić L. Supplementary material for the article: Ilić, B.; Dimkić, I.; Unković, N.; Grbić, M. L.; Vukojević, J.; Vujisić, L.; Tešević, V.; Stanković, S.; Makarov, S.; Lučić, L. Millipedes vs. Pathogens: Defensive Secretions of Some Julids (Diplopoda: Julida) as Potential Antimicrobial Agents. Journal of Applied Entomology 2018, 142 (8), 775–791. https://doi.org/10.1111/jen.12526. in Journal of Applied Entomology. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3238 .

Ilic, Bojan, Dimkić, Ivica, Unković, Nikola, Ljaljević-Grbić, Milica, Vukojević, Jelena, Vujisić, Ljubodrag V., Tešević, Vele, Stanković, Slaviša, Makarov, Slobodan E., Lučić, Luka, "Supplementary material for the article: Ilić, B.; Dimkić, I.; Unković, N.; Grbić, M. L.; Vukojević, J.; Vujisić, L.; Tešević, V.; Stanković, S.; Makarov, S.; Lučić, L. Millipedes vs. Pathogens: Defensive Secretions of Some Julids (Diplopoda: Julida) as Potential Antimicrobial Agents. Journal of Applied Entomology 2018, 142 (8), 775–791. https://doi.org/10.1111/jen.12526" in Journal of Applied Entomology (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3238 .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Guzelmeric, Etil
AU  - Trifković, Jelena
AU  - Knežević, Magdalena
AU  - Berić, Tanja
AU  - Yesilada, Erdem
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2929
AB  - Comprehensive analysis of phenolic profiles of botanically different subtypes of Turkish propolis samples were performed using UHPLC-LTQ/Orbitrap/MS/MS method, and additionally total phenolic (TPC) and total flavonoid contents (TFC) as well as their antioxidative activities were evaluated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Extensive fingerprint analysis of Turkish propolis revealed the presence of fifty one phenolic compounds, with fifteen quantified which confirm their affiliation to the two subtypes of the European propolis. All analysed samples have shown antimicrobial potential against all tested bacteria, with S. pyogenes being the most sensitive one. Turkish propolis, especially its orange subtype, can be considered as the high-quality product due to its rich phenolic and flavonoid content, strong antioxidative and antimicrobial activities. Turkish propolis could be, therefore, a good raw material for food and pharmaceutical industry.
PB  - Elsevier Science Bv, Amsterdam
T2  - LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
T1  - Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities
VL  - 95
SP  - 367
EP  - 379
DO  - 10.1016/j.lwt.2018.04.063
ER  - 

@article{
author = "Ristivojević, Petar and Dimkić, Ivica and Guzelmeric, Etil and Trifković, Jelena and Knežević, Magdalena and Berić, Tanja and Yesilada, Erdem and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2018",
abstract = "Comprehensive analysis of phenolic profiles of botanically different subtypes of Turkish propolis samples were performed using UHPLC-LTQ/Orbitrap/MS/MS method, and additionally total phenolic (TPC) and total flavonoid contents (TFC) as well as their antioxidative activities were evaluated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Extensive fingerprint analysis of Turkish propolis revealed the presence of fifty one phenolic compounds, with fifteen quantified which confirm their affiliation to the two subtypes of the European propolis. All analysed samples have shown antimicrobial potential against all tested bacteria, with S. pyogenes being the most sensitive one. Turkish propolis, especially its orange subtype, can be considered as the high-quality product due to its rich phenolic and flavonoid content, strong antioxidative and antimicrobial activities. Turkish propolis could be, therefore, a good raw material for food and pharmaceutical industry.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)",
title = "Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities",
volume = "95",
pages = "367-379",
doi = "10.1016/j.lwt.2018.04.063"
}

Ristivojević, P., Dimkić, I., Guzelmeric, E., Trifković, J., Knežević, M., Berić, T., Yesilada, E., Milojković-Opsenica, D.,& Stanković, S.. (2018). Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
Elsevier Science Bv, Amsterdam., 95, 367-379.
https://doi.org/10.1016/j.lwt.2018.04.063

Ristivojević P, Dimkić I, Guzelmeric E, Trifković J, Knežević M, Berić T, Yesilada E, Milojković-Opsenica D, Stanković S. Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie). 2018;95:367-379.
doi:10.1016/j.lwt.2018.04.063 .

Ristivojević, Petar, Dimkić, Ivica, Guzelmeric, Etil, Trifković, Jelena, Knežević, Magdalena, Berić, Tanja, Yesilada, Erdem, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities" in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie), 95 (2018):367-379,
https://doi.org/10.1016/j.lwt.2018.04.063 . .

TY  - DATA
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Guzelmeric, Etil
AU  - Trifković, Jelena
AU  - Knežević, Magdalena
AU  - Berić, Tanja
AU  - Yesilada, Erdem
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2930
PB  - Elsevier Science Bv, Amsterdam
T2  - LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
T1  - Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Guzelmeric, E.; Trifković, J.; Knežević, M.; Berić, T.; Yesilada, E.; Milojković-Opsenica, D.; Stanković, S. Profiling of Turkish Propolis Subtypes: Comparative Evaluation of Their Phytochemical Compositions, Antioxidant and Antimicrobial Activities. LWT 2018, 95, 367–379. https://doi.org/10.1016/j.lwt.2018.04.063
DO  - 10.1016/j.lwt.2018.04.063
ER  - 

@misc{
author = "Ristivojević, Petar and Dimkić, Ivica and Guzelmeric, Etil and Trifković, Jelena and Knežević, Magdalena and Berić, Tanja and Yesilada, Erdem and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)",
title = "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Guzelmeric, E.; Trifković, J.; Knežević, M.; Berić, T.; Yesilada, E.; Milojković-Opsenica, D.; Stanković, S. Profiling of Turkish Propolis Subtypes: Comparative Evaluation of Their Phytochemical Compositions, Antioxidant and Antimicrobial Activities. LWT 2018, 95, 367–379. https://doi.org/10.1016/j.lwt.2018.04.063",
doi = "10.1016/j.lwt.2018.04.063"
}

Ristivojević, P., Dimkić, I., Guzelmeric, E., Trifković, J., Knežević, M., Berić, T., Yesilada, E., Milojković-Opsenica, D.,& Stanković, S.. (2018). Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Guzelmeric, E.; Trifković, J.; Knežević, M.; Berić, T.; Yesilada, E.; Milojković-Opsenica, D.; Stanković, S. Profiling of Turkish Propolis Subtypes: Comparative Evaluation of Their Phytochemical Compositions, Antioxidant and Antimicrobial Activities. LWT 2018, 95, 367–379. https://doi.org/10.1016/j.lwt.2018.04.063. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
Elsevier Science Bv, Amsterdam..
https://doi.org/10.1016/j.lwt.2018.04.063

Ristivojević P, Dimkić I, Guzelmeric E, Trifković J, Knežević M, Berić T, Yesilada E, Milojković-Opsenica D, Stanković S. Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Guzelmeric, E.; Trifković, J.; Knežević, M.; Berić, T.; Yesilada, E.; Milojković-Opsenica, D.; Stanković, S. Profiling of Turkish Propolis Subtypes: Comparative Evaluation of Their Phytochemical Compositions, Antioxidant and Antimicrobial Activities. LWT 2018, 95, 367–379. https://doi.org/10.1016/j.lwt.2018.04.063. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie). 2018;.
doi:10.1016/j.lwt.2018.04.063 .

Ristivojević, Petar, Dimkić, Ivica, Guzelmeric, Etil, Trifković, Jelena, Knežević, Magdalena, Berić, Tanja, Yesilada, Erdem, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Guzelmeric, E.; Trifković, J.; Knežević, M.; Berić, T.; Yesilada, E.; Milojković-Opsenica, D.; Stanković, S. Profiling of Turkish Propolis Subtypes: Comparative Evaluation of Their Phytochemical Compositions, Antioxidant and Antimicrobial Activities. LWT 2018, 95, 367–379. https://doi.org/10.1016/j.lwt.2018.04.063" in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie) (2018),
https://doi.org/10.1016/j.lwt.2018.04.063 . .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Guzelmeric, Etil
AU  - Trifković, Jelena
AU  - Knežević, Magdalena
AU  - Berić, Tanja
AU  - Yesilada, Erdem
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2165
AB  - Comprehensive analysis of phenolic profiles of botanically different subtypes of Turkish propolis samples were performed using UHPLC-LTQ/Orbitrap/MS/MS method, and additionally total phenolic (TPC) and total flavonoid contents (TFC) as well as their antioxidative activities were evaluated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Extensive fingerprint analysis of Turkish propolis revealed the presence of fifty one phenolic compounds, with fifteen quantified which confirm their affiliation to the two subtypes of the European propolis. All analysed samples have shown antimicrobial potential against all tested bacteria, with S. pyogenes being the most sensitive one. Turkish propolis, especially its orange subtype, can be considered as the high-quality product due to its rich phenolic and flavonoid content, strong antioxidative and antimicrobial activities. Turkish propolis could be, therefore, a good raw material for food and pharmaceutical industry.
PB  - Elsevier Science Bv, Amsterdam
T2  - LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
T1  - Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities
VL  - 95
SP  - 367
EP  - 379
DO  - 10.1016/j.lwt.2018.04.063
ER  - 

@article{
author = "Ristivojević, Petar and Dimkić, Ivica and Guzelmeric, Etil and Trifković, Jelena and Knežević, Magdalena and Berić, Tanja and Yesilada, Erdem and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2018",
abstract = "Comprehensive analysis of phenolic profiles of botanically different subtypes of Turkish propolis samples were performed using UHPLC-LTQ/Orbitrap/MS/MS method, and additionally total phenolic (TPC) and total flavonoid contents (TFC) as well as their antioxidative activities were evaluated by spectrophotometry. Antimicrobial activity of Turkish propolis against oral cavity bacteria from the genus Streptococcus (S. pyogenes, S. sanguinis, S. mutans) and Candida albicans ATCC 10231 was determined by diffusion and microdilution methods. Extensive fingerprint analysis of Turkish propolis revealed the presence of fifty one phenolic compounds, with fifteen quantified which confirm their affiliation to the two subtypes of the European propolis. All analysed samples have shown antimicrobial potential against all tested bacteria, with S. pyogenes being the most sensitive one. Turkish propolis, especially its orange subtype, can be considered as the high-quality product due to its rich phenolic and flavonoid content, strong antioxidative and antimicrobial activities. Turkish propolis could be, therefore, a good raw material for food and pharmaceutical industry.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)",
title = "Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities",
volume = "95",
pages = "367-379",
doi = "10.1016/j.lwt.2018.04.063"
}

Ristivojević, P., Dimkić, I., Guzelmeric, E., Trifković, J., Knežević, M., Berić, T., Yesilada, E., Milojković-Opsenica, D.,& Stanković, S.. (2018). Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
Elsevier Science Bv, Amsterdam., 95, 367-379.
https://doi.org/10.1016/j.lwt.2018.04.063

Ristivojević P, Dimkić I, Guzelmeric E, Trifković J, Knežević M, Berić T, Yesilada E, Milojković-Opsenica D, Stanković S. Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie). 2018;95:367-379.
doi:10.1016/j.lwt.2018.04.063 .

Ristivojević, Petar, Dimkić, Ivica, Guzelmeric, Etil, Trifković, Jelena, Knežević, Magdalena, Berić, Tanja, Yesilada, Erdem, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Profiling of Turkish propolis subtypes: Comparative evaluation of their phytochemical compositions, antioxidant and antimicrobial activities" in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie), 95 (2018):367-379,
https://doi.org/10.1016/j.lwt.2018.04.063 . .

TY  - DATA
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Nišavić, Marija
AU  - Petković, Marijana
AU  - Ristivojević, Petar
AU  - Fira, Đorđe
AU  - Berić, Tanja
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3113
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3113
ER  - 

@misc{
author = "Dimkić, Ivica and Stanković, Slaviša and Nišavić, Marija and Petković, Marijana and Ristivojević, Petar and Fira, Đorđe and Berić, Tanja",
year = "2017",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3113"
}

Dimkić, I., Stanković, S., Nišavić, M., Petković, M., Ristivojević, P., Fira, Đ.,& Berić, T.. (2017). Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne..
https://hdl.handle.net/21.15107/rcub_cherry_3113

Dimkić I, Stanković S, Nišavić M, Petković M, Ristivojević P, Fira Đ, Berić T. Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925. in Frontiers in Microbiology. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_3113 .

Dimkić, Ivica, Stanković, Slaviša, Nišavić, Marija, Petković, Marijana, Ristivojević, Petar, Fira, Đorđe, Berić, Tanja, "Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925" in Frontiers in Microbiology (2017),
https://hdl.handle.net/21.15107/rcub_cherry_3113 .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Nišavić, Marija
AU  - Petković, Marijana
AU  - Ristivojević, Petar
AU  - Fira, Đorđe
AU  - Berić, Tanja
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2463
AB  - In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains
VL  - 8
DO  - 10.3389/fmicb.2017.00925
ER  - 

@article{
author = "Dimkić, Ivica and Stanković, Slaviša and Nišavić, Marija and Petković, Marijana and Ristivojević, Petar and Fira, Đorđe and Berić, Tanja",
year = "2017",
abstract = "In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains",
volume = "8",
doi = "10.3389/fmicb.2017.00925"
}

Dimkić, I., Stanković, S., Nišavić, M., Petković, M., Ristivojević, P., Fira, Đ.,& Berić, T.. (2017). The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.00925

Dimkić I, Stanković S, Nišavić M, Petković M, Ristivojević P, Fira Đ, Berić T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00925 .

Dimkić, Ivica, Stanković, Slaviša, Nišavić, Marija, Petković, Marijana, Ristivojević, Petar, Fira, Đorđe, Berić, Tanja, "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00925 . .

TY  - JOUR
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
AU  - Vujisić, Ljubodrag V.
AU  - Pavković-Lučić, Sofija
AU  - Jovanović, Zvezdana
AU  - Stević, Tatjana
AU  - Sofrenić, Ivana V.
AU  - Mitić, Bojan M.
AU  - Tomić, Vladimir T.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2363
AB  - The chemical defence of the millipede Pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. In total, independently of sex, 44 compounds were identified. All compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. The relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. The two dominant quinones in both sexes were 2-methyl-1,4,-benzoquinone and 2-methoxy-3-methyl-1,4-benzoquinone. Antibacterial and antifungal activity of the defensive secretion was evaluated in vitro against seven bacterial strains and eight fungal species. With the aid of a dilution technique, the antimicrobial potential of the secretion and high sensitivity of all tested strains were confirmed. The lowest minimum concentrations of these compounds (0.20-0.25 mg/mL) were sufficient for inhibition of Aeromonas hydrophila, Listeria monocytogenes and Methicillin resistant Staphylococcus aureus (MRSA). The growth of eight tested fungal species was inhibited by slightly lower concentrations of the secretion, with Fusarium equisetias the most sensitive fungus and Aspergillus flavus as the most resistant. Values of MIC and MFC in the employed microdilution assay ranged from 0.10 to above 0.35 mg/m L. The given extract contains antimicrobial components potentially useful as therapeutic agents in the pharmaceutical and agricultural industries.
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167249
ER  - 

@article{
author = "Stanković, Slaviša and Dimkić, Ivica and Vujisić, Ljubodrag V. and Pavković-Lučić, Sofija and Jovanović, Zvezdana and Stević, Tatjana and Sofrenić, Ivana V. and Mitić, Bojan M. and Tomić, Vladimir T.",
year = "2016",
abstract = "The chemical defence of the millipede Pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. In total, independently of sex, 44 compounds were identified. All compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. The relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. The two dominant quinones in both sexes were 2-methyl-1,4,-benzoquinone and 2-methoxy-3-methyl-1,4-benzoquinone. Antibacterial and antifungal activity of the defensive secretion was evaluated in vitro against seven bacterial strains and eight fungal species. With the aid of a dilution technique, the antimicrobial potential of the secretion and high sensitivity of all tested strains were confirmed. The lowest minimum concentrations of these compounds (0.20-0.25 mg/mL) were sufficient for inhibition of Aeromonas hydrophila, Listeria monocytogenes and Methicillin resistant Staphylococcus aureus (MRSA). The growth of eight tested fungal species was inhibited by slightly lower concentrations of the secretion, with Fusarium equisetias the most sensitive fungus and Aspergillus flavus as the most resistant. Values of MIC and MFC in the employed microdilution assay ranged from 0.10 to above 0.35 mg/m L. The given extract contains antimicrobial components potentially useful as therapeutic agents in the pharmaceutical and agricultural industries.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167249"
}

Stanković, S., Dimkić, I., Vujisić, L. V., Pavković-Lučić, S., Jovanović, Z., Stević, T., Sofrenić, I. V., Mitić, B. M.,& Tomić, V. T.. (2016). Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae). in PLoS One / Public Library of Science
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167249

Stanković S, Dimkić I, Vujisić LV, Pavković-Lučić S, Jovanović Z, Stević T, Sofrenić IV, Mitić BM, Tomić VT. Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae). in PLoS One / Public Library of Science. 2016;11(12).
doi:10.1371/journal.pone.0167249 .

Stanković, Slaviša, Dimkić, Ivica, Vujisić, Ljubodrag V., Pavković-Lučić, Sofija, Jovanović, Zvezdana, Stević, Tatjana, Sofrenić, Ivana V., Mitić, Bojan M., Tomić, Vladimir T., "Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)" in PLoS One / Public Library of Science, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167249 . .

TY  - DATA
AU  - Dimkić, Ivica
AU  - Ristivojević, Petar
AU  - Janakiev, Tamara
AU  - Berić, Tanja
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3593
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3593
ER  - 

@misc{
author = "Dimkić, Ivica and Ristivojević, Petar and Janakiev, Tamara and Berić, Tanja and Trifković, Jelena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3593"
}

Dimkić, I., Ristivojević, P., Janakiev, T., Berić, T., Trifković, J., Milojković-Opsenica, D.,& Stanković, S.. (2016). Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3593

Dimkić I, Ristivojević P, Janakiev T, Berić T, Trifković J, Milojković-Opsenica D, Stanković S. Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065. in Industrial Crops and Products. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3593 .

Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065" in Industrial Crops and Products (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3593 .

TY  - DATA
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Trifković, Jelena
AU  - Berić, Tanja
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3345
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3345
ER  - 

@misc{
author = "Ristivojević, Petar and Dimkić, Ivica and Trifković, Jelena and Berić, Tanja and Vovk, Irena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3345"
}

Ristivojević, P., Dimkić, I., Trifković, J., Berić, T., Vovk, I., Milojković-Opsenica, D.,& Stanković, S.. (2016). Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097. in PLoS One / Public Library of Science
Public Library Science, San Francisco..
https://hdl.handle.net/21.15107/rcub_cherry_3345

Ristivojević P, Dimkić I, Trifković J, Berić T, Vovk I, Milojković-Opsenica D, Stanković S. Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097. in PLoS One / Public Library of Science. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3345 .

Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097" in PLoS One / Public Library of Science (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3345 .

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Trifković, Jelena
AU  - Berić, Tanja
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2261
AB  - New information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as High Performance Thin-Layer Chromatography (HPTLC) fingerprinting. This study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of Serbian origin against various bacterial species. A linear multivariate calibration technique, using Partial Least Squares, was used to extract the relevant information from the chromatographic fingerprints, i.e. to indicate peaks which represent phenolic compounds that are potentially responsible for the antimicrobial capacity of the samples. In addition, direct bioautography was performed to localize the antibacterial activity on chromatograms. The biological activity of the propolis samples against various bacterial species was determined by a minimum inhibitory concentration assay, confirming their affiliation with the European poplar type of propolis and revealing the existence of two types (blue and orange) according to botanical origin. The strongest antibacterial activity was exhibited by sample 26 against Staphylococcus aureus, with a MIC value of 0.5 mg/mL, and Listeria monocytogenes, with a MIC as low as 0.1 mg/mL, which was also the lowest effective concentration observed in our study. Generally, the orange type of propolis shows higher antimicrobial activity compared to the blue type. PLS modelling was performed on the HPTLC data set and the resulting models might qualitatively indicate compounds that play an important role in the activity exhibited by the propolis samples. The most relevant peaks influencing the antimicrobial activity of propolis against all bacterial strains were phenolic compounds at RF values of 0.37, 0.40, 0.45, 0.51, 0.60 and 0.70. The knowledge gained through this study could be important for attributing the antimicrobial activity of propolis to specific chemical compounds, as well as the verification of HPTLC fingerprinting as a reliable method for the identification of compounds that are potentially responsible for antimicrobial activity. This is the first report on the activity of Serbian propolis as determined by several combined methods, including the modelling of antimicrobial activity by HPTLC fingerprinting.
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics
VL  - 11
IS  - 6
DO  - 10.1371/journal.pone.0157097
ER  - 

@article{
author = "Ristivojević, Petar and Dimkić, Ivica and Trifković, Jelena and Berić, Tanja and Vovk, Irena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
abstract = "New information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as High Performance Thin-Layer Chromatography (HPTLC) fingerprinting. This study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of Serbian origin against various bacterial species. A linear multivariate calibration technique, using Partial Least Squares, was used to extract the relevant information from the chromatographic fingerprints, i.e. to indicate peaks which represent phenolic compounds that are potentially responsible for the antimicrobial capacity of the samples. In addition, direct bioautography was performed to localize the antibacterial activity on chromatograms. The biological activity of the propolis samples against various bacterial species was determined by a minimum inhibitory concentration assay, confirming their affiliation with the European poplar type of propolis and revealing the existence of two types (blue and orange) according to botanical origin. The strongest antibacterial activity was exhibited by sample 26 against Staphylococcus aureus, with a MIC value of 0.5 mg/mL, and Listeria monocytogenes, with a MIC as low as 0.1 mg/mL, which was also the lowest effective concentration observed in our study. Generally, the orange type of propolis shows higher antimicrobial activity compared to the blue type. PLS modelling was performed on the HPTLC data set and the resulting models might qualitatively indicate compounds that play an important role in the activity exhibited by the propolis samples. The most relevant peaks influencing the antimicrobial activity of propolis against all bacterial strains were phenolic compounds at RF values of 0.37, 0.40, 0.45, 0.51, 0.60 and 0.70. The knowledge gained through this study could be important for attributing the antimicrobial activity of propolis to specific chemical compounds, as well as the verification of HPTLC fingerprinting as a reliable method for the identification of compounds that are potentially responsible for antimicrobial activity. This is the first report on the activity of Serbian propolis as determined by several combined methods, including the modelling of antimicrobial activity by HPTLC fingerprinting.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics",
volume = "11",
number = "6",
doi = "10.1371/journal.pone.0157097"
}

Ristivojević, P., Dimkić, I., Trifković, J., Berić, T., Vovk, I., Milojković-Opsenica, D.,& Stanković, S.. (2016). Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. in PLoS One / Public Library of Science
Public Library Science, San Francisco., 11(6).
https://doi.org/10.1371/journal.pone.0157097

Ristivojević P, Dimkić I, Trifković J, Berić T, Vovk I, Milojković-Opsenica D, Stanković S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. in PLoS One / Public Library of Science. 2016;11(6).
doi:10.1371/journal.pone.0157097 .

Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics" in PLoS One / Public Library of Science, 11, no. 6 (2016),
https://doi.org/10.1371/journal.pone.0157097 . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Ristivojević, Petar
AU  - Janakiev, Tamara
AU  - Berić, Tanja
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2345
AB  - Extensive employment of plant resins and propolis, as an antiseptic agents dating from ancient times in numerous cultures indicating that it may have antimicrobial and other biological properties. Resins from deciduous trees from the Populus and Salix genera, several fruit trees from the genus Prunus and a few other species were analyzed for their phenolic composition and antibacterial activity. Phenolic profiling of the plant resins was performed by high-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry. Antimicrobial activity against seven bacterial species was determined by minimum inhibitory concentration (MIC) assays and bioautography. The synergism, additivism, and antagonism of phenolic compounds were used to define the nature and type of interactions. Populus spp. showed higher amounts of p-hydroxybenzoic acid, p-coumaric acid, caffeic acid, chrysin, apigenin, quercetin, pinocembrin, pinobanksin and galangin, which confirmed the botanical origin of the orange and blue types of propolis. In addition, Gram-positive bacteria exhibited high susceptibility to poplar samples while being resistant to samples from other origins. Cherry bud samples had high amounts of naringenin and showed strong activity against Bacillus subtilis and Listeria monocytogenes. The combinations of tested phenolics showed mainly additive or indifferent effects. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis
VL  - 94
SP  - 856
EP  - 871
DO  - 10.1016/j.indcrop.2016.09.065
ER  - 

@article{
author = "Dimkić, Ivica and Ristivojević, Petar and Janakiev, Tamara and Berić, Tanja and Trifković, Jelena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
abstract = "Extensive employment of plant resins and propolis, as an antiseptic agents dating from ancient times in numerous cultures indicating that it may have antimicrobial and other biological properties. Resins from deciduous trees from the Populus and Salix genera, several fruit trees from the genus Prunus and a few other species were analyzed for their phenolic composition and antibacterial activity. Phenolic profiling of the plant resins was performed by high-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry. Antimicrobial activity against seven bacterial species was determined by minimum inhibitory concentration (MIC) assays and bioautography. The synergism, additivism, and antagonism of phenolic compounds were used to define the nature and type of interactions. Populus spp. showed higher amounts of p-hydroxybenzoic acid, p-coumaric acid, caffeic acid, chrysin, apigenin, quercetin, pinocembrin, pinobanksin and galangin, which confirmed the botanical origin of the orange and blue types of propolis. In addition, Gram-positive bacteria exhibited high susceptibility to poplar samples while being resistant to samples from other origins. Cherry bud samples had high amounts of naringenin and showed strong activity against Bacillus subtilis and Listeria monocytogenes. The combinations of tested phenolics showed mainly additive or indifferent effects. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis",
volume = "94",
pages = "856-871",
doi = "10.1016/j.indcrop.2016.09.065"
}

Dimkić, I., Ristivojević, P., Janakiev, T., Berić, T., Trifković, J., Milojković-Opsenica, D.,& Stanković, S.. (2016). Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 94, 856-871.
https://doi.org/10.1016/j.indcrop.2016.09.065

Dimkić I, Ristivojević P, Janakiev T, Berić T, Trifković J, Milojković-Opsenica D, Stanković S. Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis. in Industrial Crops and Products. 2016;94:856-871.
doi:10.1016/j.indcrop.2016.09.065 .

Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis" in Industrial Crops and Products, 94 (2016):856-871,
https://doi.org/10.1016/j.indcrop.2016.09.065 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Vukotić, Goran N.
AU  - Pastor, Ferenc
AU  - Sužnjević, Desanka
AU  - Jovanović, Živko S.
AU  - Strahinic, Ivana D.
AU  - Fira, Dorde A.
AU  - Radovic, Svetlana S.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1961
AB  - Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria
VL  - 67
IS  - 3
SP  - 1033
EP  - 1042
DO  - 10.2298/ABS150130066S
ER  - 

@article{
author = "Stanisavljević, Nemanja S. and Vukotić, Goran N. and Pastor, Ferenc and Sužnjević, Desanka and Jovanović, Živko S. and Strahinic, Ivana D. and Fira, Dorde A. and Radovic, Svetlana S.",
year = "2015",
abstract = "Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria",
volume = "67",
number = "3",
pages = "1033-1042",
doi = "10.2298/ABS150130066S"
}

Stanisavljević, N. S., Vukotić, G. N., Pastor, F., Sužnjević, D., Jovanović, Ž. S., Strahinic, I. D., Fira, D. A.,& Radovic, S. S.. (2015). Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 67(3), 1033-1042.
https://doi.org/10.2298/ABS150130066S

Stanisavljević NS, Vukotić GN, Pastor F, Sužnjević D, Jovanović ŽS, Strahinic ID, Fira DA, Radovic SS. Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria. in Archives of biological sciences. 2015;67(3):1033-1042.
doi:10.2298/ABS150130066S .

Stanisavljević, Nemanja S., Vukotić, Goran N., Pastor, Ferenc, Sužnjević, Desanka, Jovanović, Živko S., Strahinic, Ivana D., Fira, Dorde A., Radovic, Svetlana S., "Antioxidant Activity of Pea Protein Hydrolysates Produced by Batch Fermentation with Lactic Acid Bacteria" in Archives of biological sciences, 67, no. 3 (2015):1033-1042,
https://doi.org/10.2298/ABS150130066S . .